Status
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Gene information
General information
TC010260 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 28499637..28503381
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC010260
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_01662

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      beetle stock: bad (increased lethality),
      Pupal morphology
      beetle stock: bad (increased lethality),
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC010260 RA
        MAARSVFKL ITKTCSYTL NPTCPSTFS SDKCYRKGQ 
        RSYSQPPKH QFRRKTKQD FYQLRQLSK SLQNLRDSR 
        FKEDEFEEF EAEEKPRFS VTPVDTSFR ETAKEFKEK 
        YVPEEKNDE GNPIYELGE CSRSEENYY QVFHHFKKS 
        EDNKMKRLF MHLQPCNLR PYGSNDNLF YPNFSKKKI 
        KIVYPSAPR TGESEPSRR KFDGIHYKE FSSYRLEKK 
        ESTGHEDGK KLSLGEKSS SKMIINKKN YSTSCMKPR 
        KTLTKSGVD LKGPKLTVC KSLTRRSST ALVKKHKKE 
        TKKSQLDAI VKSNIATLE QLTKKINES RTKRLNREQ 
        SNTCGWSRS YNTYNKPAD INCDDYVTV KLSELKEEC 
        RKLKKCKPK KISFGRLPK LTSPDCPCI EDVPLKEGK 
        PLIRLKKRF DVKEPKNVC ESELVCTTP RADDNYKVV 
        PKTLPVIQP QECPCEERE MTDFVIKRL PKKHIPEPK 
        RICSSCDDE ICCAPRADA SYKHKVKPL PKLKPNNCP 
        CIEEIPLKN VPLHRLKAK PIKEPERKC SELHVCEIP 
        RADDGLKVE QKVLPTIKS ECVCVEPPP LEPGTPLPR 
        LPKFKYVEK PRESCDLPP CYDAVRADG DLKVTAKKL 
        PVFVPQNCP CKEEVIPDA PVKMKRLKM RNIPKEKKK 
        VCVEEVCGE RADDGMKIK PKQLPVLEP KECPCVPTE 
        MKDAPPLKK LVPKPVQER PRPCLVVTE CTTPRADEK 
        LKVQKKSLP VLPPGACPC IEPEPPLDV KPLKKLKKK 
        PVVFKRVCT VVEECTTER ADENLKVKV KKLPKLDVP 
        DCPCIEPSP PEDVKPLAR LKKVHIPEP KRICPVVDS 
        CEEATRADE DDWLYWKRV EVKPPECEP KKTNKRSFS 
        TVARRFYCT STKNNDLFQ LKYDVPQLS VSEFGAKPY 
        NVIIDIKPS APLILTQQP LKSHQVEGF DTSLARKRS 
        KRSRSEPVD CVTFVIYKS NSLGASKSV KEPSSDVVL 
        FSNNSRFIS TCSTLYEYS ERKDKLKDH VTPSCECPP 
        NDRHLQPRN PIIPEKETF ITQYPADEL VDHAAKKSR 
        MYYKTRKAD VDSHERKEE AEDECKEEE QERAKAICE 
        VGKDGTTKV EKQLTLWEK IVNYFKARP CPSPEEFRK 
        MRLREDAER AASKAGLCL YDPKEMLRK NEKELPKVI 
        TASSARDDC QK
      CDS sequence
      • >TC010260 RA
        atggcagca cgctcggtt ttcaaactc attactaaa 
        acgtgttcc tacaccttg aatccaaca tgtccatcc 
        acattttcc tccgacaag tgctacaga aagggacaa 
        cgatcctat tcacaacct cccaagcac caatttcgg 
        cgcaaaacc aaacaggac ttttaccaa ttgcgccaa 
        ttgtccaaa tccttgcag aacctcaga gatagtcgt 
        ttcaaagag gacgagttt gaagaattc gaagctgaa 
        gaaaaacca aggtttagt gtcacacca gttgataca 
        agttttcgc gaaactgcg aaagaattc aaggagaag 
        tacgtccct gaggagaaa aacgacgaa gggaatccg 
        atctatgaa ctgggcgag tgctcacgc agtgaggag 
        aactactac caagtgttc caccatttc aagaagtcg 
        gaagacaat aaaatgaag cggctcttc atgcacttg 
        caaccgtgc aatttgcga ccttatggc tcaaatgac 
        aatctcttc tatcccaac tttagcaaa aagaagatc 
        aaaattgtc taccccagt gctccgaga accggcgaa 
        agcgaacca agccggcga aagtttgac ggaatccac 
        tacaaggag tttagcagt tacagactg gaaaagaaa 
        gagtccact gggcatgaa gatggaaaa aagttgtcc 
        cttggtgag aaaagctcc tccaaaatg atcatcaat 
        aagaagaac tatagtacc tcctgtatg aaaccacgg 
        aagacttta accaaatct ggagtggac ttaaaaggg 
        cccaagtta acagtttgc aaaagcttg actcgaaga 
        tctagcaca gctttggtc aaaaaacac aaaaaggaa 
        acgaaaaaa agtcaattg gacgcaatc gtcaaatct 
        aacattgca acacttgaa caattgact aaaaaaatc 
        aacgaaagc cggaccaaa agactgaac cgtgaacaa 
        tccaacact tgtggctgg agtcgcagt tacaacact 
        tacaacaaa ccagctgac atcaactgt gacgattac 
        gtgactgtt aaactaagc gagttgaag gaggaatgc 
        cgtaagttg aaaaaatgc aaaccgaag aagatttct 
        tttggtcgc cttccaaaa ctaacctcg cctgattgc 
        ccctgcatt gaggatgtg ccactgaaa gaaggcaaa 
        cctttgata cgtctcaag aaacgtttc gatgtcaaa 
        gaacccaaa aacgtgtgt gagtctgaa ctggtttgt 
        acaacacca agagctgac gataactac aaagttgtt 
        ccaaaaaca cttccagtt atccaaccg caggaatgt 
        ccatgtgaa gaacgagaa atgaccgat tttgttatt 
        aaaagactc ccaaagaag catattcca gaaccgaaa 
        cgaatttgt tcttcatgt gatgatgaa atttgttgt 
        gctccaaga gccgacgct tcgtacaaa cacaaagtc 
        aaacctttg cccaaactt aaacctaac aattgtcct 
        tgtattgaa gaaatacca ctgaagaat gtccccttg 
        caccgattg aaggccaaa ccaatcaaa gaaccggaa 
        cggaaatgc tcagaacta catgtttgt gaaatacca 
        agagctgat gatggtctt aaagtggaa cagaaggta 
        ctaccaacc atcaagagt gagtgtgtt tgtgtggaa 
        ccaccacca cttgaaccc ggtactcca ttaccacgc 
        ctacctaaa tttaaatac gtggaaaag ccacgggaa 
        agctgtgac cttccgccg tgttacgac gccgttcga 
        gctgatggt gatttgaaa gttacagcg aaaaaactc 
        ccagttttc gtcccccag aactgtccg tgcaaagaa 
        gaagtgatt ccagacgcc ccagttaag atgaaacga 
        ctcaaaatg agaaatatc cccaaagaa aagaagaaa 
        gtttgtgtg gaggaagtt tgtggtgaa cgagccgat 
        gatgggatg aaaatcaaa ccaaaacaa cttccagtt 
        ttggaaccc aaggagtgt ccttgcgtt cccaccgaa 
        atgaaggac gcaccaccg ttgaaaaaa ttggtacca 
        aagccagta caagagcgt ccaagacct tgcctggtc 
        gtaacagag tgtacgaca ccacgagct gacgaaaaa 
        ctcaaagtc cagaagaaa tccttgccg gttttgccc 
        cccggagcg tgtccttgt atcgaaccg gagcctcca 
        ctcgatgtc aagccgttg aagaagttg aagaaaaaa 
        ccggtggtg tttaaacgt gtttgcact gttgtggaa 
        gagtgtacg actgaacgt gcggatgaa aatctcaaa 
        gttaaagtg aagaaattg ccaaagttg gacgttcct 
        gattgtcct tgcattgaa ccgtcgccg cctgaagac 
        gtcaagcct ttggcaagg ttgaaaaag gtccatatt 
        cccgagcct aagagaatt tgccctgtg gttgatagt 
        tgtgaggag gcgacaaga gccgatgaa gacgattgg 
        ttgtattgg aagagggtt gaggtgaaa ccgccagag 
        tgtgagccg aagaaaacc aacaagagg agttttagt 
        accgttgct agaagattt tattgtact agtaccaag 
        aacaacgat ttgttccaa ttgaagtat gacgttccc 
        cagttatcg gttagtgag tttggggcg aaaccttac 
        aacgttatc attgatatc aagccttcg gctcctttg 
        attttgacg caacagccg ttgaaaagc caccaagtc 
        gaaggtttt gatacgagt ttagcccgg aaacggtca 
        aaacgttca cggtctgaa ccagtcgat tgtgtcact 
        tttgtcatc tataagagc aacagtctt ggtgcttct 
        aaatcagtg aaggaaccg tcgagtgat gttgtgcta 
        ttttcaaac aacagtcgt tttatctcg acttgtagt 
        actctctac gagtactcg gaacgtaag gataagttg 
        aaggatcat gtaactcca tcctgtgaa tgtcctcca 
        aatgataga catctccag cctcgaaac cccataatt 
        ccagaaaag gaaactttt atcacccaa tatccggcc 
        gatgagctt gttgatcat gcggccaaa aaaagcagg 
        atgtactac aagaccagg aaagctgat gtggattca 
        cacgagagg aaggaagaa gcggaagat gagtgtaag 
        gaagaagaa caagaaagg gcaaaggca atttgtgag 
        gttgggaag gatgggacc acaaaagtg gaaaaacag 
        ttaaccctt tgggaaaaa atcgtcaac tacttcaag 
        gcaaggccg tgtccgagc ccagaagag tttaggaag 
        atgcggttg agggaagat gccgaaagg gcggcgtca 
        aaagctggt ttgtgtctg tatgaccct aaggagatg 
        ctgcggaag aatgagaag gagttgccg aaggtgata 
        acagcgtcg tcggcgcgg gacgactgt cagaaatga
      mRNA sequence
      • >TC010260 RA
        tttaactaa aaaattaga ttttttgac gtgtctaaa 
        tcctggaaa attatcgta ttatggcag cacgctcgg 
        ttttcaaac tcattacta aaacgtgtt cctacacct 
        tgaatccaa catgtccat ccacatttt cctccgaca 
        agtgctaca gaaagggac aacgatcct attcacaac 
        ctcccaagc accaatttc ggcgcaaaa ccaaacagg 
        acttttacc aattgcgcc aattgtcca aatccttgc 
        agaacctca gagatagtc gtttcaaag aggacgagt 
        ttgaagaat tcgaagctg aagaaaaac caaggttta 
        gtgtcacac cagttgata caagttttc gcgaaactg 
        cgaaagaat tcaaggaga agtacgtcc ctgaggaga 
        aaaacgacg aagggaatc cgatctatg aactgggcg 
        agtgctcac gcagtgagg agaactact accaagtgt 
        tccaccatt tcaagaagt cggaagaca ataaaatga 
        agcggctct tcatgcact tgcaaccgt gcaatttgc 
        gaccttatg gctcaaatg acaatctct tctatccca 
        actttagca aaaagaaga tcaaaattg tctacccca 
        gtgctccga gaaccggcg aaagcgaac caagccggc 
        gaaagtttg acggaatcc actacaagg agtttagca 
        gttacagac tggaaaaga aagagtcca ctgggcatg 
        aagatggaa aaaagttgt cccttggtg agaaaagct 
        cctccaaaa tgatcatca ataagaaga actatagta 
        cctcctgta tgaaaccac ggaagactt taaccaaat 
        ctggagtgg acttaaaag ggcccaagt taacagttt 
        gcaaaagct tgactcgaa gatctagca cagctttgg 
        tcaaaaaac acaaaaagg aaacgaaaa aaagtcaat 
        tggacgcaa tcgtcaaat ctaacattg caacacttg 
        aacaattga ctaaaaaaa tcaacgaaa gccggacca 
        aaagactga accgtgaac aatccaaca cttgtggct 
        ggagtcgca gttacaaca cttacaaca aaccagctg 
        acatcaact gtgacgatt acgtgactg ttaaactaa 
        gcgagttga aggaggaat gccgtaagt tgaaaaaat 
        gcaaaccga agaagattt cttttggtc gccttccaa 
        aactaacct cgcctgatt gcccctgca ttgaggatg 
        tgccactga aagaaggca aacctttga tacgtctca 
        agaaacgtt tcgatgtca aagaaccca aaaacgtgt 
        gtgagtctg aactggttt gtacaacac caagagctg 
        acgataact acaaagttg ttccaaaaa cacttccag 
        ttatccaac cgcaggaat gtccatgtg aagaacgag 
        aaatgaccg attttgtta ttaaaagac tcccaaaga 
        agcatattc cagaaccga aacgaattt gttcttcat 
        gtgatgatg aaatttgtt gtgctccaa gagccgacg 
        cttcgtaca aacacaaag tcaaacctt tgcccaaac 
        ttaaaccta acaattgtc cttgtattg aagaaatac 
        cactgaaga atgtcccct tgcaccgat tgaaggcca 
        aaccaatca aagaaccgg aacggaaat gctcagaac 
        tacatgttt gtgaaatac caagagctg atgatggtc 
        ttaaagtgg aacagaagg tactaccaa ccatcaaga 
        gtgagtgtg tttgtgtgg aaccaccac cacttgaac 
        ccggtactc cattaccac gcctaccta aatttaaat 
        acgtggaaa agccacggg aaagctgtg accttccgc 
        cgtgttacg acgccgttc gagctgatg gtgatttga 
        aagttacag cgaaaaaac tcccagttt tcgtccccc 
        agaactgtc cgtgcaaag aagaagtga ttccagacg 
        ccccagtta agatgaaac gactcaaaa tgagaaata 
        tccccaaag aaaagaaga aagtttgtg tggaggaag 
        tttgtggtg aacgagccg atgatggga tgaaaatca 
        aaccaaaac aacttccag ttttggaac ccaaggagt 
        gtccttgcg ttcccaccg aaatgaagg acgcaccac 
        cgttgaaaa aattggtac caaagccag tacaagagc 
        gtccaagac cttgcctgg tcgtaacag agtgtacga 
        caccacgag ctgacgaaa aactcaaag tccagaaga 
        aatccttgc cggttttgc cccccggag cgtgtcctt 
        gtatcgaac cggagcctc cactcgatg tcaagccgt 
        tgaagaagt tgaagaaaa aaccggtgg tgtttaaac 
        gtgtttgca ctgttgtgg aagagtgta cgactgaac 
        gtgcggatg aaaatctca aagttaaag tgaagaaat 
        tgccaaagt tggacgttc ctgattgtc cttgcattg 
        aaccgtcgc cgcctgaag acgtcaagc ctttggcaa 
        ggttgaaaa aggtccata ttcccgagc ctaagagaa 
        tttgccctg tggttgata gttgtgagg aggcgacaa 
        gagccgatg aagacgatt ggttgtatt ggaagaggg 
        ttgaggtga aaccgccag agtgtgagc cgaagaaaa 
        ccaacaaga ggagtttta gtaccgttg ctagaagat 
        tttattgta ctagtacca agaacaacg atttgttcc 
        aattgaagt atgacgttc cccagttat cggttagtg 
        agtttgggg cgaaacctt acaacgtta tcattgata 
        tcaagcctt cggctcctt tgattttga cgcaacagc 
        cgttgaaaa gccaccaag tcgaaggtt ttgatacga 
        gtttagccc ggaaacggt caaaacgtt cacggtctg 
        aaccagtcg attgtgtca cttttgtca tctataaga 
        gcaacagtc ttggtgctt ctaaatcag tgaaggaac 
        cgtcgagtg atgttgtgc tattttcaa acaacagtc 
        gttttatct cgacttgta gtactctct acgagtact 
        cggaacgta aggataagt tgaaggatc atgtaactc 
        catcctgtg aatgtcctc caaatgata gacatctcc 
        agcctcgaa accccataa ttccagaaa aggaaactt 
        ttatcaccc aatatccgg ccgatgagc ttgttgatc 
        atgcggcca aaaaaagca ggatgtact acaagacca 
        ggaaagctg atgtggatt cacacgaga ggaaggaag 
        aagcggaag atgagtgta aggaagaag aacaagaaa 
        gggcaaagg caatttgtg aggttggga aggatggga 
        ccacaaaag tggaaaaac agttaaccc tttgggaaa 
        aaatcgtca actacttca aggcaaggc cgtgtccga 
        gcccagaag agtttagga agatgcggt tgagggaag 
        atgccgaaa gggcggcgt caaaagctg gtttgtgtc 
        tgtatgacc ctaaggaga tgctgcgga agaatgaga 
        aggagttgc cgaaggtga taacagcgt cgtcggcgc 
        gggacgact gtcagaaat gaaatgtat tagtttttt 
        gttttgaat atatgctct gagtaaga
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY