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Gene information
General information
TC010245 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid584: 60779..68033
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010245
    iBeetle screen iB_01660
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010245 RA
      MMSVFTEFW PQILVFLAL FLLVLVNYF NYCYRYWSK 
      KGVATIKPR FPLGNTNII LPQGLSIGI LSKQFYDEF 
      KAKGHKFGG VYLVTEPNL LIVDPEYVK DIMSKDFQH 
      FVDRGFYYN KEKDVLSAH LFAIDGSDW RNLRIKLTP 
      TFTSGKMKM MFEAVVQCS KHMIENLDK NLGSDIEIK 
      EVLGRFTTD VIGTCAFGI ECNSFKYPD AEFRMMGKK 
      IFEYDLWKS VKAFFAVNV PKVALQLGL TSTPDDVID 
      FFTRIVADA VKMRESNNI RRNDFLQIL IDLKNTTSL 
      TLDEMSAQV FLFFVAGFE TSSTTMTFA LYELARNEK 
      MQEKLRFEI CQVLDKTGG QITYESLIE MKYLQQVID 
      ETLRMYPPL PTLNRRCTK DYVLRDTNI IIEKDTPIL 
      ISALGLHMD PEFFPKPEK FDPERFTEE KKKERHPFV 
      HLPFGDGPR NCIGLRFGV MQSKIGIIT IIKNFKLTV 
      SSNMKPVQF NPYVFLLNI NDKVLLQVG KL
    CDS sequence
    • >TC010245 RA
      atgatgagt gtttttacc gaattttgg ccacaaatt 
      ttggtcttc cttgcattg tttttgcta gttttggtc 
      aactacttc aattattgt taccgatat tggtcaaaa 
      aaaggcgtt gcaacaatt aaaccccga tttccactg 
      ggcaacacc aacattatc ctgcctcaa ggtttgtca 
      attgggatt ttgagcaag caattttac gatgaattc 
      aaagcgaaa ggacacaag tttggaggt gtttatcta 
      gtgaccgaa ccaaatctc ctgattgtt gatccagaa 
      tatgttaag gatattatg tctaaagat ttccaacat 
      tttgttgat cgtgggttt tactacaac aaggaaaag 
      gatgtttta agtgctcac ttattcgct attgatggt 
      tctgattgg cgaaacctc cgaattaag ctaacacca 
      acatttact tcgggaaaa atgaaaatg atgtttgag 
      gcagttgtc caatgttcc aaacacatg attgaaaat 
      ttagacaag aaccttggc tctgacata gaaatcaaa 
      gaagtttta ggccgattt acaaccgat gtaatcggc 
      acatgtgct tttggtatt gaatgtaac agttttaag 
      tacccagat gctgaattc agaatgatg ggtaaaaaa 
      atttttgaa tacgattta tggaagtct gttaaagca 
      ttttttgct gtcaatgta ccaaaagtc gccttacaa 
      cttggatta acgtcaaca ccagacgat gttatagat 
      ttttttaca agaattgtt gccgatgca gtaaaaatg 
      cgtgaaagt aacaatatc agaagaaat gattttctt 
      caaattttg atcgatctg aaaaacacc acaagtttg 
      actcttgac gaaatgtca gctcaagtg tttttgttt 
      ttcgtagcc ggttttgag accagctca acaaccatg 
      acgtttgct ttgtacgaa cttgccaga aatgaaaaa 
      atgcaggag aaactaaga tttgagatt tgccaagtt 
      ttggacaag actggcggc caaatcacg tatgagagt 
      ctaattgaa atgaaatac ttgcagcaa gttattgat 
      gaaactttg cgaatgtac ccaccactg ccaacactt 
      aataggcgc tgtaccaaa gactatgtt ctacgtgac 
      accaatatc atcattgag aaggacacc ccgatttta 
      atttccgct ttagggctt catatggat ccggagttt 
      ttcccgaaa ccagaaaaa ttcgacccg gaaaggttc 
      acagaagag aaaaagaaa gaaagacat ccctttgtg 
      catttgcca tttggtgat ggtcccaga aactgtatt 
      ggcttgcgc tttggggtc atgcaatca aaaattgga 
      atcattaca attattaaa aattttaaa ttgacggtt 
      agttctaat atgaaacca gtacagttc aatccttat 
      gtttttctt ttgaatatt aatgacaag gttttactg 
      caagttggg aaattgtga
    mRNA sequence
    • >TC010245 RA
      aatctctgt aattataag agatacgag tttcaacaa 
      ccgcgtcat gatgagtgt ttttaccga attttggcc 
      acaaatttt ggtcttcct tgcattgtt tttgctagt 
      tttggtcaa ctacttcaa ttattgtta ccgatattg 
      gtcaaaaaa aggcgttgc aacaattaa accccgatt 
      tccactggg caacaccaa cattatcct gcctcaagg 
      tttgtcaat tgggatttt gagcaagca attttacga 
      tgaattcaa agcgaaagg acacaagtt tggaggtgt 
      ttatctagt gaccgaacc aaatctcct gattgttga 
      tccagaata tgttaagga tattatgtc taaagattt 
      ccaacattt tgttgatcg tgggtttta ctacaacaa 
      ggaaaagga tgttttaag tgctcactt attcgctat 
      tgatggttc tgattggcg aaacctccg aattaagct 
      aacaccaac atttacttc gggaaaaat gaaaatgat 
      gtttgaggc agttgtcca atgttccaa acacatgat 
      tgaaaattt agacaagaa ccttggctc tgacataga 
      aatcaaaga agttttagg ccgatttac aaccgatgt 
      aatcggcac atgtgcttt tggtattga atgtaacag 
      ttttaagta cccagatgc tgaattcag aatgatggg 
      taaaaaaat ttttgaata cgatttatg gaagtctgt 
      taaagcatt ttttgctgt caatgtacc aaaagtcgc 
      cttacaact tggattaac gtcaacacc agacgatgt 
      tatagattt ttttacaag aattgttgc cgatgcagt 
      aaaaatgcg tgaaagtaa caatatcag aagaaatga 
      ttttcttca aattttgat cgatctgaa aaacaccac 
      aagtttgac tcttgacga aatgtcagc tcaagtgtt 
      tttgttttt cgtagccgg ttttgagac cagctcaac 
      aaccatgac gtttgcttt gtacgaact tgccagaaa 
      tgaaaaaat gcaggagaa actaagatt tgagatttg 
      ccaagtttt ggacaagac tggcggcca aatcacgta 
      tgagagtct aattgaaat gaaatactt gcagcaagt 
      tattgatga aactttgcg aatgtaccc accactgcc 
      aacacttaa taggcgctg taccaaaga ctatgttct 
      acgtgacac caatatcat cattgagaa ggacacccc 
      gattttaat ttccgcttt agggcttca tatggatcc 
      ggagttttt cccgaaacc agaaaaatt cgacccgga 
      aaggttcac agaagagaa aaagaaaga aagacatcc 
      ctttgtgca tttgccatt tggtgatgg tcccagaaa 
      ctgtattgg cttgcgctt tggggtcat gcaatcaaa 
      aattggaat cattacaat tattaaaaa ttttaaatt 
      gacggttag ttctaatat gaaaccagt acagttcaa 
      tccttatgt ttttctttt gaatattaa tgacaaggt 
      tttactgca agttgggaa attgtgaca tttgcaaaa 
      attgaagat tttgtttga tttctttat gtaacatca 
      tcgccggct ttatacata catttcatt aaggaggcg 
      gtgtgtaac ataatgaag gaataagtt tctgattat 
      aataggtac tgtcaataa aaatatatt agaaaa