Status
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Gene information
General information
TC033846
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010171
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13650039..13653391
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033846
    iBeetle screen iB_01656

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033846 RA
      MGFRLVLLL VFLYGGKCE VKQDLGGEW SLKDETEEY 
      KDLSATVPG GIYTDLMNP SNKILGNIY AGFNDIEYK 
      WVARYNWTY STTFQVQED LTKRDVINL VLEGVDTFA 
      TIFINDVKV GTTENMFVR YIFEITNTL KAGVNTITL 
      KFDSPITVS EELAKKQAE DYAVPPDCV PDSYKGECH 
      VNFIRKIPA SFSWDWGPA FPSVGIWKD IYLEAFYVT 
      TIRYVVAEV IDSGDNWLL SIDTYFAPK TTGITGKLD 
      VSLQTKREP IQTSSEVIL GEPNEHGEI FVTTNVTVP 
      KSSVDLWWP NGYGKQPLY TLNVTFTND KESSTSQKK 
      IGFRKIEIM QDELDSGLA FYFKVNNVP IYAMGVNVI 
      PLDILPEES YKRETVDRL LAAVAEYTE MNMLRVWGG 
      GVYESDYFY EMADKYGLM IWQDFVFAS AMYPANAEF 
      LSNVQREVN HQVKRIQSH ASLAVWGGN NENEGNLAS 
      NHFNTADNF DVYKADYIK LYIDTVRDE MRRATKNAN 
      FVNSSPTNG IQTDKEGYV ASDPGSNFY GDKHYYNNK 
      KDSLDESIF PTPRFSSEY GYQSLPSVT SWLTVANSS 
      VLNLNSDFM KHRQHHPDG NDPLKNLIE FQMELPSED 
      NEYYDEAVI YYTQIIQAM SVKTETEHY RRFMGRFDD 
      QGQGNTMGA LFWQLNDVW VAPSWASID YTGRWKMLH 
      NFVPNFFSH ELVTGHYNK DSKKLEIYH MLNKPNVDG 
      EKWINLTVY SWESFTPLN VTQLIGEFE PLVARLVAS 
      YDVDQFLSD SGCGAADVA KYNCFVHIV GGKDGTVLT 
      PSNYFFPSK FKEAKLKNS NVEIISVDM IDAKGLRFH 
      VWVKADAPS LFVWLDPHD IKGHFLENG FIQHSLVTT 
      VTFIADSPT TSEDLKQAL TVTHLRDPK YFISRK
    CDS sequence
    • >TC033846 RA
      atgggattt cgactagtt ctgcttttg gttttcctc 
      tacggggga aagtgtgaa gttaagcag gatttgggg 
      ggcgagtgg tcgctcaaa gatgagact gaagaatac 
      aaagatttg tcggcaacg gtcccgggt gggatttac 
      accgattta atgaaccca tcaaacaaa attctgggc 
      aatatttac gccggtttt aatgatatt gagtacaaa 
      tgggtggcc agatacaac tggacttat tccaccaca 
      ttccaagtg caagaagat ctaaccaaa cgtgacgtt 
      atcaatttg gttcttgag ggtgtggac acgttcgcc 
      acaattttt atcaatgac gttaaagtg ggcacgacc 
      gaaaatatg ttcgtgcgt tacattttt gaaatcacc 
      aatacactc aaggccggt gttaacacc atcacttta 
      aagttcgat tctccgatt acggttagt gaggaattg 
      gccaagaaa caggccgaa gactacgct gtgcccccc 
      gattgtgtc cccgattcg tacaagggt gaatgtcat 
      gttaatttt attcggaaa attccggct tcgttttcc 
      tgggactgg gggccagct ttcccctca gttgggatt 
      tggaaagac atttacttg gaggctttt tacgtaact 
      acgatcaga tacgtagtt gctgaagtc atagacagt 
      ggagacaac tggcttttg tcaattgat acgtatttt 
      gcccccaag acaactggg attacaggc aagctggac 
      gtttcgtta caaaccaaa cgtgaacca atccaaacc 
      tccagtgag gtaatactg ggtgaacca aacgaacat 
      ggggaaatt ttcgtaaca actaatgta acagtacct 
      aaatcgtcg gttgactta tggtggccc aatggttac 
      ggtaaacaa cccctttac accctcaat gtgactttt 
      accaatgac aaagaatcg agcacgagt cagaaaaaa 
      attgggttt aggaaaatt gagataatg caagatgag 
      cttgactcg ggccttgcg ttttatttc aaagtgaac 
      aatgtcccg atttatgca atgggtgtt aacgtcatc 
      cctcttgat atcctcccg gaagagagc tacaaaagg 
      gaaactgtt gatcgttta ctcgccgct gtggccgaa 
      tacactgaa atgaacatg ttgcgagtg tggggcggg 
      ggcgtttac gagtcggat tatttttac gaaatggcc 
      gataaatac ggactgatg atttggcaa gacttcgtt 
      tttgcctcg gccatgtac cccgcaaat gccgaattt 
      ctgagcaat gtgcagcgg gaagtaaat caccaagtt 
      aagaggatc caaagtcac gcgagtctg gccgtttgg 
      ggcggcaat aacgagaac gagggcaat ttagcatcg 
      aaccatttc aataccgct gataatttc gatgtttac 
      aaggcggat tacataaag ctgtacatc gacactgtt 
      agggacgaa atgaggcga gcaacgaaa aatgctaat 
      tttgtcaat tcgagtccc acgaatggg attcaaacg 
      gacaaggag ggatacgtt gcgagtgat cctggcagc 
      aatttttac ggcgacaag cattattat aacaataag 
      aaagacagt ctggacgaa agtattttt cccacaccg 
      agattttcg tccgagtac ggctaccag tcgttacca 
      agtgttact tcctggctc accgttgcc aattcaagt 
      gtcttaaac cttaacagc gactttatg aaacacaga 
      caacaccac cctgatgga aacgatccg ctgaaaaac 
      cttatcgaa ttccagatg gagctccca agcgaggat 
      aacgaatat tacgatgaa gctgtcatt tattacaca 
      caaattatt caagcaatg tctgtaaaa accgaaacg 
      gagcattat cgccgattt atggggcga ttcgatgac 
      caaggccag ggcaacacg atgggggcc ctcttctgg 
      caactcaac gacgtttgg gtggcccct agttgggcc 
      tcgattgat tacacgggc aggtggaaa atgctgcac 
      aattttgtc cccaatttc ttttcgcac gaattagtc 
      acgggccat tacaataaa gatagcaaa aaattggaa 
      atttatcac atgttgaat aaaccaaat gtggatggc 
      gaaaagtgg atcaatttg acggtttat tcgtgggag 
      tcgttcacc cctctcaac gttacccaa cttatcgga 
      gaatttgag cctttggtg gcaagacta gttgcatca 
      tacgatgtg gaccaattt ttgtccgat tccgggtgt 
      ggcgctgca gatgtagca aaatacaac tgctttgtg 
      catattgtt ggtgggaaa gacggcaca gttttgacc 
      ccgtctaat tactttttc ccttcaaaa tttaaggaa 
      gctaaactt aaaaactcc aatgttgag attatttca 
      gttgatatg attgacgcg aaaggcttg cgcttccac 
      gtgtgggtg aaagccgac gcccctagt cttttcgtt 
      tggttggac ccgcacgat attaaaggc cactttctt 
      gaaaacggt tttattcaa cactctctt gttacaaca 
      gtcactttt atagcagat tcaccaaca acaagcgaa 
      gatttgaaa caagcgctt acagtgact catctaaga 
      gaccctaag tattttatt tcacggaaa taa
    mRNA sequence
    • >TC033846 RA
      caactaggt gtttgaaga tgggatttc gactagttc 
      tgcttttgg ttttcctct acgggggaa agtgtgaag 
      ttaagcagg atttggggg gcgagtggt cgctcaaag 
      atgagactg aagaataca aagatttgt cggcaacgg 
      tcccgggtg ggatttaca ccgatttaa tgaacccat 
      caaacaaaa ttctgggca atatttacg ccggtttta 
      atgatattg agtacaaat gggtggcca gatacaact 
      ggacttatt ccaccacat tccaagtgc aagaagatc 
      taaccaaac gtgacgtta tcaatttgg ttcttgagg 
      gtgtggaca cgttcgcca caattttta tcaatgacg 
      ttaaagtgg gcacgaccg aaaatatgt tcgtgcgtt 
      acatttttg aaatcacca atacactca aggccggtg 
      ttaacacca tcactttaa agttcgatt ctccgatta 
      cggttagtg aggaattgg ccaagaaac aggccgaag 
      actacgctg tgccccccg attgtgtcc ccgattcgt 
      acaagggtg aatgtcatg ttaatttta ttcggaaaa 
      ttccggctt cgttttcct gggactggg ggccagctt 
      tcccctcag ttgggattt ggaaagaca tttacttgg 
      aggcttttt acgtaacta cgatcagat acgtagttg 
      ctgaagtca tagacagtg gagacaact ggcttttgt 
      caattgata cgtattttg cccccaaga caactggga 
      ttacaggca agctggacg tttcgttac aaaccaaac 
      gtgaaccaa tccaaacct ccagtgagg taatactgg 
      gtgaaccaa acgaacatg gggaaattt tcgtaacaa 
      ctaatgtaa cagtaccta aatcgtcgg ttgacttat 
      ggtggccca atggttacg gtaaacaac ccctttaca 
      ccctcaatg tgactttta ccaatgaca aagaatcga 
      gcacgagtc agaaaaaaa ttgggttta ggaaaattg 
      agataatgc aagatgagc ttgactcgg gccttgcgt 
      tttatttca aagtgaaca atgtcccga tttatgcaa 
      tgggtgtta acgtcatcc ctcttgata tcctcccgg 
      aagagagct acaaaaggg aaactgttg atcgtttac 
      tcgccgctg tggccgaat acactgaaa tgaacatgt 
      tgcgagtgt ggggcgggg gcgtttacg agtcggatt 
      atttttacg aaatggccg ataaatacg gactgatga 
      tttggcaag acttcgttt ttgcctcgg ccatgtacc 
      ccgcaaatg ccgaatttc tgagcaatg tgcagcggg 
      aagtaaatc accaagtta agaggatcc aaagtcacg 
      cgagtctgg ccgtttggg gcggcaata acgagaacg 
      agggcaatt tagcatcga accatttca ataccgctg 
      ataatttcg atgtttaca aggcggatt acataaagc 
      tgtacatcg acactgtta gggacgaaa tgaggcgag 
      caacgaaaa atgctaatt ttgtcaatt cgagtccca 
      cgaatggga ttcaaacgg acaaggagg gatacgttg 
      cgagtgatc ctggcagca atttttacg gcgacaagc 
      attattata acaataaga aagacagtc tggacgaaa 
      gtatttttc ccacaccga gattttcgt ccgagtacg 
      gctaccagt cgttaccaa gtgttactt cctggctca 
      ccgttgcca attcaagtg tcttaaacc ttaacagcg 
      actttatga aacacagac aacaccacc ctgatggaa 
      acgatccgc tgaaaaacc ttatcgaat tccagatgg 
      agctcccaa gcgaggata acgaatatt acgatgaag 
      ctgtcattt attacacac aaattattc aagcaatgt 
      ctgtaaaaa ccgaaacgg agcattatc gccgattta 
      tggggcgat tcgatgacc aaggccagg gcaacacga 
      tgggggccc tcttctggc aactcaacg acgtttggg 
      tggccccta gttgggcct cgattgatt acacgggca 
      ggtggaaaa tgctgcaca attttgtcc ccaatttct 
      tttcgcacg aattagtca cgggccatt acaataaag 
      atagcaaaa aattggaaa tttatcaca tgttgaata 
      aaccaaatg tggatggcg aaaagtgga tcaatttga 
      cggtttatt cgtgggagt cgttcaccc ctctcaacg 
      ttacccaac ttatcggag aatttgagc ctttggtgg 
      caagactag ttgcatcat acgatgtgg accaatttt 
      tgtccgatt ccgggtgtg gcgctgcag atgtagcaa 
      aatacaact gctttgtgc atattgttg gtgggaaag 
      acggcacag ttttgaccc cgtctaatt actttttcc 
      cttcaaaat ttaaggaag ctaaactta aaaactcca 
      atgttgaga ttatttcag ttgatatga ttgacgcga 
      aaggcttgc gcttccacg tgtgggtga aagccgacg 
      cccctagtc ttttcgttt ggttggacc cgcacgata 
      ttaaaggcc actttcttg aaaacggtt ttattcaac 
      actctcttg ttacaacag tcactttta tagcagatt 
      caccaacaa caagcgaag atttgaaac aagcgctta 
      cagtgactc atctaagag accctaagt attttattt 
      cacggaaat aagtaatac atgaattaa taattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY