General information | |||
TC033846
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 13650039..13653391 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
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TC033846 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01656 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC033846 RA
MGFRLVLLL VFLYGGKCE VKQDLGGEW SLKDETEEY KDLSATVPG GIYTDLMNP SNKILGNIY AGFNDIEYK WVARYNWTY STTFQVQED LTKRDVINL VLEGVDTFA TIFINDVKV GTTENMFVR YIFEITNTL KAGVNTITL KFDSPITVS EELAKKQAE DYAVPPDCV PDSYKGECH VNFIRKIPA SFSWDWGPA FPSVGIWKD IYLEAFYVT TIRYVVAEV IDSGDNWLL SIDTYFAPK TTGITGKLD VSLQTKREP IQTSSEVIL GEPNEHGEI FVTTNVTVP KSSVDLWWP NGYGKQPLY TLNVTFTND KESSTSQKK IGFRKIEIM QDELDSGLA FYFKVNNVP IYAMGVNVI PLDILPEES YKRETVDRL LAAVAEYTE MNMLRVWGG GVYESDYFY EMADKYGLM IWQDFVFAS AMYPANAEF LSNVQREVN HQVKRIQSH ASLAVWGGN NENEGNLAS NHFNTADNF DVYKADYIK LYIDTVRDE MRRATKNAN FVNSSPTNG IQTDKEGYV ASDPGSNFY GDKHYYNNK KDSLDESIF PTPRFSSEY GYQSLPSVT SWLTVANSS VLNLNSDFM KHRQHHPDG NDPLKNLIE FQMELPSED NEYYDEAVI YYTQIIQAM SVKTETEHY RRFMGRFDD QGQGNTMGA LFWQLNDVW VAPSWASID YTGRWKMLH NFVPNFFSH ELVTGHYNK DSKKLEIYH MLNKPNVDG EKWINLTVY SWESFTPLN VTQLIGEFE PLVARLVAS YDVDQFLSD SGCGAADVA KYNCFVHIV GGKDGTVLT PSNYFFPSK FKEAKLKNS NVEIISVDM IDAKGLRFH VWVKADAPS LFVWLDPHD IKGHFLENG FIQHSLVTT VTFIADSPT TSEDLKQAL TVTHLRDPK YFISRK
- >TC033846 RA
atgggattt cgactagtt ctgcttttg gttttcctc tacggggga aagtgtgaa gttaagcag gatttgggg ggcgagtgg tcgctcaaa gatgagact gaagaatac aaagatttg tcggcaacg gtcccgggt gggatttac accgattta atgaaccca tcaaacaaa attctgggc aatatttac gccggtttt aatgatatt gagtacaaa tgggtggcc agatacaac tggacttat tccaccaca ttccaagtg caagaagat ctaaccaaa cgtgacgtt atcaatttg gttcttgag ggtgtggac acgttcgcc acaattttt atcaatgac gttaaagtg ggcacgacc gaaaatatg ttcgtgcgt tacattttt gaaatcacc aatacactc aaggccggt gttaacacc atcacttta aagttcgat tctccgatt acggttagt gaggaattg gccaagaaa caggccgaa gactacgct gtgcccccc gattgtgtc cccgattcg tacaagggt gaatgtcat gttaatttt attcggaaa attccggct tcgttttcc tgggactgg gggccagct ttcccctca gttgggatt tggaaagac atttacttg gaggctttt tacgtaact acgatcaga tacgtagtt gctgaagtc atagacagt ggagacaac tggcttttg tcaattgat acgtatttt gcccccaag acaactggg attacaggc aagctggac gtttcgtta caaaccaaa cgtgaacca atccaaacc tccagtgag gtaatactg ggtgaacca aacgaacat ggggaaatt ttcgtaaca actaatgta acagtacct aaatcgtcg gttgactta tggtggccc aatggttac ggtaaacaa cccctttac accctcaat gtgactttt accaatgac aaagaatcg agcacgagt cagaaaaaa attgggttt aggaaaatt gagataatg caagatgag cttgactcg ggccttgcg ttttatttc aaagtgaac aatgtcccg atttatgca atgggtgtt aacgtcatc cctcttgat atcctcccg gaagagagc tacaaaagg gaaactgtt gatcgttta ctcgccgct gtggccgaa tacactgaa atgaacatg ttgcgagtg tggggcggg ggcgtttac gagtcggat tatttttac gaaatggcc gataaatac ggactgatg atttggcaa gacttcgtt tttgcctcg gccatgtac cccgcaaat gccgaattt ctgagcaat gtgcagcgg gaagtaaat caccaagtt aagaggatc caaagtcac gcgagtctg gccgtttgg ggcggcaat aacgagaac gagggcaat ttagcatcg aaccatttc aataccgct gataatttc gatgtttac aaggcggat tacataaag ctgtacatc gacactgtt agggacgaa atgaggcga gcaacgaaa aatgctaat tttgtcaat tcgagtccc acgaatggg attcaaacg gacaaggag ggatacgtt gcgagtgat cctggcagc aatttttac ggcgacaag cattattat aacaataag aaagacagt ctggacgaa agtattttt cccacaccg agattttcg tccgagtac ggctaccag tcgttacca agtgttact tcctggctc accgttgcc aattcaagt gtcttaaac cttaacagc gactttatg aaacacaga caacaccac cctgatgga aacgatccg ctgaaaaac cttatcgaa ttccagatg gagctccca agcgaggat aacgaatat tacgatgaa gctgtcatt tattacaca caaattatt caagcaatg tctgtaaaa accgaaacg gagcattat cgccgattt atggggcga ttcgatgac caaggccag ggcaacacg atgggggcc ctcttctgg caactcaac gacgtttgg gtggcccct agttgggcc tcgattgat tacacgggc aggtggaaa atgctgcac aattttgtc cccaatttc ttttcgcac gaattagtc acgggccat tacaataaa gatagcaaa aaattggaa atttatcac atgttgaat aaaccaaat gtggatggc gaaaagtgg atcaatttg acggtttat tcgtgggag tcgttcacc cctctcaac gttacccaa cttatcgga gaatttgag cctttggtg gcaagacta gttgcatca tacgatgtg gaccaattt ttgtccgat tccgggtgt ggcgctgca gatgtagca aaatacaac tgctttgtg catattgtt ggtgggaaa gacggcaca gttttgacc ccgtctaat tactttttc ccttcaaaa tttaaggaa gctaaactt aaaaactcc aatgttgag attatttca gttgatatg attgacgcg aaaggcttg cgcttccac gtgtgggtg aaagccgac gcccctagt cttttcgtt tggttggac ccgcacgat attaaaggc cactttctt gaaaacggt tttattcaa cactctctt gttacaaca gtcactttt atagcagat tcaccaaca acaagcgaa gatttgaaa caagcgctt acagtgact catctaaga gaccctaag tattttatt tcacggaaa taa
- >TC033846 RA
caactaggt gtttgaaga tgggatttc gactagttc tgcttttgg ttttcctct acgggggaa agtgtgaag ttaagcagg atttggggg gcgagtggt cgctcaaag atgagactg aagaataca aagatttgt cggcaacgg tcccgggtg ggatttaca ccgatttaa tgaacccat caaacaaaa ttctgggca atatttacg ccggtttta atgatattg agtacaaat gggtggcca gatacaact ggacttatt ccaccacat tccaagtgc aagaagatc taaccaaac gtgacgtta tcaatttgg ttcttgagg gtgtggaca cgttcgcca caattttta tcaatgacg ttaaagtgg gcacgaccg aaaatatgt tcgtgcgtt acatttttg aaatcacca atacactca aggccggtg ttaacacca tcactttaa agttcgatt ctccgatta cggttagtg aggaattgg ccaagaaac aggccgaag actacgctg tgccccccg attgtgtcc ccgattcgt acaagggtg aatgtcatg ttaatttta ttcggaaaa ttccggctt cgttttcct gggactggg ggccagctt tcccctcag ttgggattt ggaaagaca tttacttgg aggcttttt acgtaacta cgatcagat acgtagttg ctgaagtca tagacagtg gagacaact ggcttttgt caattgata cgtattttg cccccaaga caactggga ttacaggca agctggacg tttcgttac aaaccaaac gtgaaccaa tccaaacct ccagtgagg taatactgg gtgaaccaa acgaacatg gggaaattt tcgtaacaa ctaatgtaa cagtaccta aatcgtcgg ttgacttat ggtggccca atggttacg gtaaacaac ccctttaca ccctcaatg tgactttta ccaatgaca aagaatcga gcacgagtc agaaaaaaa ttgggttta ggaaaattg agataatgc aagatgagc ttgactcgg gccttgcgt tttatttca aagtgaaca atgtcccga tttatgcaa tgggtgtta acgtcatcc ctcttgata tcctcccgg aagagagct acaaaaggg aaactgttg atcgtttac tcgccgctg tggccgaat acactgaaa tgaacatgt tgcgagtgt ggggcgggg gcgtttacg agtcggatt atttttacg aaatggccg ataaatacg gactgatga tttggcaag acttcgttt ttgcctcgg ccatgtacc ccgcaaatg ccgaatttc tgagcaatg tgcagcggg aagtaaatc accaagtta agaggatcc aaagtcacg cgagtctgg ccgtttggg gcggcaata acgagaacg agggcaatt tagcatcga accatttca ataccgctg ataatttcg atgtttaca aggcggatt acataaagc tgtacatcg acactgtta gggacgaaa tgaggcgag caacgaaaa atgctaatt ttgtcaatt cgagtccca cgaatggga ttcaaacgg acaaggagg gatacgttg cgagtgatc ctggcagca atttttacg gcgacaagc attattata acaataaga aagacagtc tggacgaaa gtatttttc ccacaccga gattttcgt ccgagtacg gctaccagt cgttaccaa gtgttactt cctggctca ccgttgcca attcaagtg tcttaaacc ttaacagcg actttatga aacacagac aacaccacc ctgatggaa acgatccgc tgaaaaacc ttatcgaat tccagatgg agctcccaa gcgaggata acgaatatt acgatgaag ctgtcattt attacacac aaattattc aagcaatgt ctgtaaaaa ccgaaacgg agcattatc gccgattta tggggcgat tcgatgacc aaggccagg gcaacacga tgggggccc tcttctggc aactcaacg acgtttggg tggccccta gttgggcct cgattgatt acacgggca ggtggaaaa tgctgcaca attttgtcc ccaatttct tttcgcacg aattagtca cgggccatt acaataaag atagcaaaa aattggaaa tttatcaca tgttgaata aaccaaatg tggatggcg aaaagtgga tcaatttga cggtttatt cgtgggagt cgttcaccc ctctcaacg ttacccaac ttatcggag aatttgagc ctttggtgg caagactag ttgcatcat acgatgtgg accaatttt tgtccgatt ccgggtgtg gcgctgcag atgtagcaa aatacaact gctttgtgc atattgttg gtgggaaag acggcacag ttttgaccc cgtctaatt actttttcc cttcaaaat ttaaggaag ctaaactta aaaactcca atgttgaga ttatttcag ttgatatga ttgacgcga aaggcttgc gcttccacg tgtgggtga aagccgacg cccctagtc ttttcgttt ggttggacc cgcacgata ttaaaggcc actttcttg aaaacggtt ttattcaac actctcttg ttacaacag tcactttta tagcagatt caccaacaa caagcgaag atttgaaac aagcgctta cagtgactc atctaagag accctaagt attttattt cacggaaat aagtaatac atgaattaa taattt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
