Status
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Gene information
General information
TC033846
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010171 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13650039..13653391
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033846
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01656

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033846 RA
      MGFRLVLLL VFLYGGKCE VKQDLGGEW SLKDETEEY 
KDLSATVPG GIYTDLMNP SNKILGNIY AGFNDIEYK 
WVARYNWTY STTFQVQED LTKRDVINL VLEGVDTFA 
TIFINDVKV GTTENMFVR YIFEITNTL KAGVNTITL 
KFDSPITVS EELAKKQAE DYAVPPDCV PDSYKGECH 
VNFIRKIPA SFSWDWGPA FPSVGIWKD IYLEAFYVT 
TIRYVVAEV IDSGDNWLL SIDTYFAPK TTGITGKLD 
VSLQTKREP IQTSSEVIL GEPNEHGEI FVTTNVTVP 
KSSVDLWWP NGYGKQPLY TLNVTFTND KESSTSQKK 
IGFRKIEIM QDELDSGLA FYFKVNNVP IYAMGVNVI 
PLDILPEES YKRETVDRL LAAVAEYTE MNMLRVWGG 
GVYESDYFY EMADKYGLM IWQDFVFAS AMYPANAEF 
LSNVQREVN HQVKRIQSH ASLAVWGGN NENEGNLAS 
NHFNTADNF DVYKADYIK LYIDTVRDE MRRATKNAN 
FVNSSPTNG IQTDKEGYV ASDPGSNFY GDKHYYNNK 
KDSLDESIF PTPRFSSEY GYQSLPSVT SWLTVANSS 
VLNLNSDFM KHRQHHPDG NDPLKNLIE FQMELPSED 
NEYYDEAVI YYTQIIQAM SVKTETEHY RRFMGRFDD 
QGQGNTMGA LFWQLNDVW VAPSWASID YTGRWKMLH 
NFVPNFFSH ELVTGHYNK DSKKLEIYH MLNKPNVDG 
EKWINLTVY SWESFTPLN VTQLIGEFE PLVARLVAS 
YDVDQFLSD SGCGAADVA KYNCFVHIV GGKDGTVLT 
PSNYFFPSK FKEAKLKNS NVEIISVDM IDAKGLRFH 
VWVKADAPS LFVWLDPHD IKGHFLENG FIQHSLVTT 
VTFIADSPT TSEDLKQAL TVTHLRDPK YFISRK
    CDS sequence
    • >TC033846 RA
      atgggattt cgactagtt ctgcttttg gttttcctc 
tacggggga aagtgtgaa gttaagcag gatttgggg 
ggcgagtgg tcgctcaaa gatgagact gaagaatac 
aaagatttg tcggcaacg gtcccgggt gggatttac 
accgattta atgaaccca tcaaacaaa attctgggc 
aatatttac gccggtttt aatgatatt gagtacaaa 
tgggtggcc agatacaac tggacttat tccaccaca 
ttccaagtg caagaagat ctaaccaaa cgtgacgtt 
atcaatttg gttcttgag ggtgtggac acgttcgcc 
acaattttt atcaatgac gttaaagtg ggcacgacc 
gaaaatatg ttcgtgcgt tacattttt gaaatcacc 
aatacactc aaggccggt gttaacacc atcacttta 
aagttcgat tctccgatt acggttagt gaggaattg 
gccaagaaa caggccgaa gactacgct gtgcccccc 
gattgtgtc cccgattcg tacaagggt gaatgtcat 
gttaatttt attcggaaa attccggct tcgttttcc 
tgggactgg gggccagct ttcccctca gttgggatt 
tggaaagac atttacttg gaggctttt tacgtaact 
acgatcaga tacgtagtt gctgaagtc atagacagt 
ggagacaac tggcttttg tcaattgat acgtatttt 
gcccccaag acaactggg attacaggc aagctggac 
gtttcgtta caaaccaaa cgtgaacca atccaaacc 
tccagtgag gtaatactg ggtgaacca aacgaacat 
ggggaaatt ttcgtaaca actaatgta acagtacct 
aaatcgtcg gttgactta tggtggccc aatggttac 
ggtaaacaa cccctttac accctcaat gtgactttt 
accaatgac aaagaatcg agcacgagt cagaaaaaa 
attgggttt aggaaaatt gagataatg caagatgag 
cttgactcg ggccttgcg ttttatttc aaagtgaac 
aatgtcccg atttatgca atgggtgtt aacgtcatc 
cctcttgat atcctcccg gaagagagc tacaaaagg 
gaaactgtt gatcgttta ctcgccgct gtggccgaa 
tacactgaa atgaacatg ttgcgagtg tggggcggg 
ggcgtttac gagtcggat tatttttac gaaatggcc 
gataaatac ggactgatg atttggcaa gacttcgtt 
tttgcctcg gccatgtac cccgcaaat gccgaattt 
ctgagcaat gtgcagcgg gaagtaaat caccaagtt 
aagaggatc caaagtcac gcgagtctg gccgtttgg 
ggcggcaat aacgagaac gagggcaat ttagcatcg 
aaccatttc aataccgct gataatttc gatgtttac 
aaggcggat tacataaag ctgtacatc gacactgtt 
agggacgaa atgaggcga gcaacgaaa aatgctaat 
tttgtcaat tcgagtccc acgaatggg attcaaacg 
gacaaggag ggatacgtt gcgagtgat cctggcagc 
aatttttac ggcgacaag cattattat aacaataag 
aaagacagt ctggacgaa agtattttt cccacaccg 
agattttcg tccgagtac ggctaccag tcgttacca 
agtgttact tcctggctc accgttgcc aattcaagt 
gtcttaaac cttaacagc gactttatg aaacacaga 
caacaccac cctgatgga aacgatccg ctgaaaaac 
cttatcgaa ttccagatg gagctccca agcgaggat 
aacgaatat tacgatgaa gctgtcatt tattacaca 
caaattatt caagcaatg tctgtaaaa accgaaacg 
gagcattat cgccgattt atggggcga ttcgatgac 
caaggccag ggcaacacg atgggggcc ctcttctgg 
caactcaac gacgtttgg gtggcccct agttgggcc 
tcgattgat tacacgggc aggtggaaa atgctgcac 
aattttgtc cccaatttc ttttcgcac gaattagtc 
acgggccat tacaataaa gatagcaaa aaattggaa 
atttatcac atgttgaat aaaccaaat gtggatggc 
gaaaagtgg atcaatttg acggtttat tcgtgggag 
tcgttcacc cctctcaac gttacccaa cttatcgga 
gaatttgag cctttggtg gcaagacta gttgcatca 
tacgatgtg gaccaattt ttgtccgat tccgggtgt 
ggcgctgca gatgtagca aaatacaac tgctttgtg 
catattgtt ggtgggaaa gacggcaca gttttgacc 
ccgtctaat tactttttc ccttcaaaa tttaaggaa 
gctaaactt aaaaactcc aatgttgag attatttca 
gttgatatg attgacgcg aaaggcttg cgcttccac 
gtgtgggtg aaagccgac gcccctagt cttttcgtt 
tggttggac ccgcacgat attaaaggc cactttctt 
gaaaacggt tttattcaa cactctctt gttacaaca 
gtcactttt atagcagat tcaccaaca acaagcgaa 
gatttgaaa caagcgctt acagtgact catctaaga 
gaccctaag tattttatt tcacggaaa taa
    mRNA sequence
    • >TC033846 RA
      caactaggt gtttgaaga tgggatttc gactagttc 
tgcttttgg ttttcctct acgggggaa agtgtgaag 
ttaagcagg atttggggg gcgagtggt cgctcaaag 
atgagactg aagaataca aagatttgt cggcaacgg 
tcccgggtg ggatttaca ccgatttaa tgaacccat 
caaacaaaa ttctgggca atatttacg ccggtttta 
atgatattg agtacaaat gggtggcca gatacaact 
ggacttatt ccaccacat tccaagtgc aagaagatc 
taaccaaac gtgacgtta tcaatttgg ttcttgagg 
gtgtggaca cgttcgcca caattttta tcaatgacg 
ttaaagtgg gcacgaccg aaaatatgt tcgtgcgtt 
acatttttg aaatcacca atacactca aggccggtg 
ttaacacca tcactttaa agttcgatt ctccgatta 
cggttagtg aggaattgg ccaagaaac aggccgaag 
actacgctg tgccccccg attgtgtcc ccgattcgt 
acaagggtg aatgtcatg ttaatttta ttcggaaaa 
ttccggctt cgttttcct gggactggg ggccagctt 
tcccctcag ttgggattt ggaaagaca tttacttgg 
aggcttttt acgtaacta cgatcagat acgtagttg 
ctgaagtca tagacagtg gagacaact ggcttttgt 
caattgata cgtattttg cccccaaga caactggga 
ttacaggca agctggacg tttcgttac aaaccaaac 
gtgaaccaa tccaaacct ccagtgagg taatactgg 
gtgaaccaa acgaacatg gggaaattt tcgtaacaa 
ctaatgtaa cagtaccta aatcgtcgg ttgacttat 
ggtggccca atggttacg gtaaacaac ccctttaca 
ccctcaatg tgactttta ccaatgaca aagaatcga 
gcacgagtc agaaaaaaa ttgggttta ggaaaattg 
agataatgc aagatgagc ttgactcgg gccttgcgt 
tttatttca aagtgaaca atgtcccga tttatgcaa 
tgggtgtta acgtcatcc ctcttgata tcctcccgg 
aagagagct acaaaaggg aaactgttg atcgtttac 
tcgccgctg tggccgaat acactgaaa tgaacatgt 
tgcgagtgt ggggcgggg gcgtttacg agtcggatt 
atttttacg aaatggccg ataaatacg gactgatga 
tttggcaag acttcgttt ttgcctcgg ccatgtacc 
ccgcaaatg ccgaatttc tgagcaatg tgcagcggg 
aagtaaatc accaagtta agaggatcc aaagtcacg 
cgagtctgg ccgtttggg gcggcaata acgagaacg 
agggcaatt tagcatcga accatttca ataccgctg 
ataatttcg atgtttaca aggcggatt acataaagc 
tgtacatcg acactgtta gggacgaaa tgaggcgag 
caacgaaaa atgctaatt ttgtcaatt cgagtccca 
cgaatggga ttcaaacgg acaaggagg gatacgttg 
cgagtgatc ctggcagca atttttacg gcgacaagc 
attattata acaataaga aagacagtc tggacgaaa 
gtatttttc ccacaccga gattttcgt ccgagtacg 
gctaccagt cgttaccaa gtgttactt cctggctca 
ccgttgcca attcaagtg tcttaaacc ttaacagcg 
actttatga aacacagac aacaccacc ctgatggaa 
acgatccgc tgaaaaacc ttatcgaat tccagatgg 
agctcccaa gcgaggata acgaatatt acgatgaag 
ctgtcattt attacacac aaattattc aagcaatgt 
ctgtaaaaa ccgaaacgg agcattatc gccgattta 
tggggcgat tcgatgacc aaggccagg gcaacacga 
tgggggccc tcttctggc aactcaacg acgtttggg 
tggccccta gttgggcct cgattgatt acacgggca 
ggtggaaaa tgctgcaca attttgtcc ccaatttct 
tttcgcacg aattagtca cgggccatt acaataaag 
atagcaaaa aattggaaa tttatcaca tgttgaata 
aaccaaatg tggatggcg aaaagtgga tcaatttga 
cggtttatt cgtgggagt cgttcaccc ctctcaacg 
ttacccaac ttatcggag aatttgagc ctttggtgg 
caagactag ttgcatcat acgatgtgg accaatttt 
tgtccgatt ccgggtgtg gcgctgcag atgtagcaa 
aatacaact gctttgtgc atattgttg gtgggaaag 
acggcacag ttttgaccc cgtctaatt actttttcc 
cttcaaaat ttaaggaag ctaaactta aaaactcca 
atgttgaga ttatttcag ttgatatga ttgacgcga 
aaggcttgc gcttccacg tgtgggtga aagccgacg 
cccctagtc ttttcgttt ggttggacc cgcacgata 
ttaaaggcc actttcttg aaaacggtt ttattcaac 
actctcttg ttacaacag tcactttta tagcagatt 
caccaacaa caagcgaag atttgaaac aagcgctta 
cagtgactc atctaagag accctaagt attttattt 
cacggaaat aagtaatac atgaattaa taattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY