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Gene information
General information
TC010125 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 14344891..14347068
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010125
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01653

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages sometimes shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    head irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs segment shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: effect is clearly stronger in the T3 legs than in the others
    legs segment potentiallyfused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs pretarsus bent - Penetrance (percentage of animals/eggs on slide): less than 30%
    prothoracic leg not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010125 RA
      MSTPQKTNS PVITTQVVT GQPIIKEGK KQTVLESHG 
      YILGKTIGS GSYATVRMA HSERHNMNV AVKIVSKFS 
      APADYLKKF LPREIEVVK GLKHPNLIR FLQAIETTH 
      RVYIIMEYA ENGSLLEII RKDSYIDEI RSRKWFRQL 
      VDAVDYCHE RGVVHRDIK CENLLLNTE YEIKLSDFG 
      FARGHMKIK NGQAPLSET FCGSYAYAS PEILKGIPY 
      QPQFSDIWS MGVVLFAMV YGRLPFDDT NYRDLVKQV 
      TNKVVFPKE PKVSLMCKA LINKILAPL KARIRIPGI 
      KTDPWYTYN TNDGSHGSR ERNDPERSG NVSTASIPK 
      ET
    CDS sequence
    • >TC010125 RA
      atgtctact ccccaaaaa accaacagt ccagtaatc 
      accactcaa gtggtaaca ggccagccg ataatcaaa 
      gagggcaaa aagcaaacc gttcttgag tctcatggc 
      tacattctg ggcaaaacg atcggctcc ggctcgtac 
      gccacggtg cggatggcc cacagcgag cggcacaac 
      atgaacgtc gccgtcaaa atcgtaagc aaattttca 
      gcaccggca gactacttg aagaaattt ctaccgaga 
      gagattgag gtggtgaaa ggactcaaa caccccaac 
      ttgatccgg ttcttgcaa gccatcgaa acgacccac 
      agagtgtat atcataatg gaatatgcc gaaaacggg 
      agtcttttg gaaataatc cgcaaggat tcgtacatt 
      gacgaaatc cggtcgcgg aagtggttc cggcagttg 
      gtcgatgct gtggactac tgccacgag cgaggcgtg 
      gtccacaga gacatcaag tgcgaaaat ctgctcctc 
      aatactgaa tatgagata aaactttcg gattttggc 
      ttcgccagg ggccatatg aagatcaaa aacgggcaa 
      gcgcccttg agcgagacc ttctgcggg agctacgct 
      tacgcctcc cccgaaatt ctgaagggc atcccctac 
      cagccccag ttttccgac atctggtcg atgggggtc 
      gtgctcttc gccatggtt tacggccgt ttgcccttc 
      gatgacacc aattaccgc gatttggtc aagcaagtc 
      acgaacaag gtggttttc ccgaaagaa ccgaaagtg 
      tcgctgatg tgcaaggcg ttgataaat aaaatttta 
      gcgccgctt aaagccagg attaggatt ccggggatc 
      aagaccgat ccttggtac acgtacaat accaacgac 
      gggagccat ggaagcagg gagcgaaat gatcccgag 
      cgaagcgga aacgtcagc actgcgtct attccgaag 
      gagacttga
    mRNA sequence
    • >TC010125 RA
      tcactatat ccgtgtcat tcaaaattt cagtgaaaa 
      tgaaataaa ccattgtag cttatagtg acatgattt 
      ggtcttaca agttatgtc tactcccca aaaaaccaa 
      cagtccagt aatcaccac tcaagtggt aacaggcca 
      gccgataat caaagaggg caaaaagca aaccgttct 
      tgagtctca tggctacat tctgggcaa aacgatcgg 
      ctccggctc gtacgccac ggtgcggat ggcccacag 
      cgagcggca caacatgaa cgtcgccgt caaaatcgt 
      aagcaaatt ttcagcacc ggcagacta cttgaagaa 
      atttctacc gagagagat tgaggtggt gaaaggact 
      caaacaccc caacttgat ccggttctt gcaagccat 
      cgaaacgac ccacagagt gtatatcat aatggaata 
      tgccgaaaa cgggagtct tttggaaat aatccgcaa 
      ggattcgta cattgacga aatccggtc gcggaagtg 
      gttccggca gttggtcga tgctgtgga ctactgcca 
      cgagcgagg cgtggtcca cagagacat caagtgcga 
      aaatctgct cctcaatac tgaatatga gataaaact 
      ttcggattt tggcttcgc caggggcca tatgaagat 
      caaaaacgg gcaagcgcc cttgagcga gaccttctg 
      cgggagcta cgcttacgc ctcccccga aattctgaa 
      gggcatccc ctaccagcc ccagttttc cgacatctg 
      gtcgatggg ggtcgtgct cttcgccat ggtttacgg 
      ccgtttgcc cttcgatga caccaatta ccgcgattt 
      ggtcaagca agtcacgaa caaggtggt tttcccgaa 
      agaaccgaa agtgtcgct gatgtgcaa ggcgttgat 
      aaataaaat tttagcgcc gcttaaagc caggattag 
      gattccggg gatcaagac cgatccttg gtacacgta 
      caataccaa cgacgggag ccatggaag cagggagcg 
      aaatgatcc cgagcgaag cggaaacgt cagcactgc 
      gtctattcc gaaggagac ttgagtcgg aggtgataa 
      accagggaa attgaatta agtgtctgg tataaagtc 
      ggttttatt atcgtttta taagactgg ctgattata 
      aaatataca agaatactt acattactg acgttattg 
      ttgtttgct tgagtgtgc ataactgtg aagcgaaat 
      gaaaatttt tcagaacca tatgcgact agaaattgc 
      tgcatcgga tcgagtgac aaagcaagg tacaaaaat 
      atcaatttg ggcgcacgg tttatttac aaaattgca 
      gcgcttaaa gtggccgct tgggccacg tcgttagat 
      ctttggtta aaaatttct agtatccaa tggcactat 
      taaaaagat aaaaaatta aaatagttt atcacat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY