Status
Please wait ...
Gene information
General information
TC010109 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 14791773..14797942
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010109
    iBeetle screen iB_01650

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010109 RA
      MRVAIIGAG AAGLASARH VSAQGIECE VIEMGSEVG 
      GTWVYTDEV GTDRFGYPV HTAMYKGLR ANLPKEIMG 
      FPDFPIPEP NGSYLDQAT ILRFLNLYA EHFNLKPLI 
      KFNHIVTEV RPNADKWSI KAKNKITKT EFASIYDVV 
      MICTGHYNT PISPSLSGQ EKFKGHVMH SHQYRSNKP 
      FQNQRVLVI GAGPSGLDV AFQVAEIAQ QVVLSYDMT 
      KKEVKGEYP SNLVKKPQV LRVKDKEHV EFVDGSCCS 
      FDTIIYCTG YRYNFPFLH HDCGVSVGD FHVRPLYKH 
      LIHIEKPTM CFIGIPYYV GAFQMFDIQ ARFYCQYLN 
      GSMSLPTKE MMYKDTEED VVKRKNKGY SEKQMHLLG 
      HDQQTYFEE LASTAKITP ILPVICKIW SDSDNSFYK 
      DLKNFRKYK YRILNNNTF VSERYIE
    CDS sequence
    • >TC010109 RA
      atgcgcgtt gctattatt ggtgctggt gctgccggt 
      ttggcctcc gcaagacac gtctcagct caagggatc 
      gaatgtgag gtgattgaa atgggctct gaggtgggg 
      ggcacctgg gtgtacacc gacgaggtg ggaacggac 
      aggtttgga tatccggta catactgca atgtacaag 
      ggcctaagg gcaaatctg ccgaaagag attatgggt 
      ttccccgac tttcccatt ccggagcca aatggctcg 
      tatttagac caagcgacc attttgcgt tttttgaat 
      ctctacgcc gaacatttc aacttaaaa ccactaatt 
      aaattcaat cacattgtg accgaagtt cgcccaaat 
      gcggacaaa tggtcaatt aaggccaaa aataaaatt 
      acaaaaacc gagtttgca agtatttac gacgtggtg 
      atgatctgt accggacat tataacact ccaatcagt 
      ccaagtttg tcgggtcaa gaaaaattc aaaggccac 
      gttatgcac agtcatcag tacagatcg aacaaaccg 
      tttcaaaat caaagagtt ttggtgatc ggtgctggg 
      ccttcaggc ctagacgtt gctttccaa gttgctgaa 
      attgctcaa caagtcgtt ctcagctat gacatgact 
      aagaaagaa gtcaagggg gaatatccc tccaacctg 
      gtcaaaaaa ccccaagtc cttcgagtc aaagacaag 
      gaacatgtt gagtttgtt gacggttct tgttgttcc 
      ttcgacacg attatatac tgcacaggg tacagatat 
      aacttcccg tttctccac catgactgt ggagtgtcc 
      gtaggagat ttccatgtt agaccactt tacaaacac 
      ttgattcat atcgaaaaa cccacaatg tgtttcatt 
      ggtattcca tattatgtt ggtgctttc caaatgttt 
      gacattcag gctcgcttc tactgccag tacctcaac 
      ggttcaatg tcgctgcct accaaagaa atgatgtac 
      aaagacacc gaagaagat gtagttaaa cgaaaaaat 
      aaaggatat tccgaaaag cagatgcac ttgttgggt 
      cacgatcaa caaacgtat ttcgaagag ttggcaagc 
      actgcaaaa attactcca atattgccc gttatttgc 
      aagatatgg agcgatagt gacaatagt ttttacaaa 
      gatctcaaa aattttaga aagtacaag tacagaatc 
      ctcaataat aacacattt gtttccgaa cgctacatc 
      gaataa
    mRNA sequence
    • >TC010109 RA
      cgcttgccg acattgaga tgtattctt cattactct 
      tattatttc cacacaacg agataattt aagtaattt 
      aacctttat tctagtatg tagtatgca cagggtaat 
      tcaagattg tagtcaagc tcactttag cactctttt 
      tatgatatt gccagaaca gaaacaatg gacacaata 
      attttaaag ataaagatg tttagttcg taaaccaca 
      gttttgtgt ctctaatcg ctcgcttta aacgtactt 
      ttgacttgt acaaaactt ttaaaaata ccacaaaaa 
      tgcgcgttg ctattattg gtgctggtg ctgccggtt 
      tggcctccg caagacacg tctcagctc aagggatcg 
      aatgtgagg tgattgaaa tgggctctg aggtggggg 
      gcacctggg tgtacaccg acgaggtgg gaacggaca 
      ggtttggat atccggtac atactgcaa tgtacaagg 
      gcctaaggg caaatctgc cgaaagaga ttatgggtt 
      tccccgact ttcccattc cggagccaa atggctcgt 
      atttagacc aagcgacca ttttgcgtt ttttgaatc 
      tctacgccg aacatttca acttaaaac cactaatta 
      aattcaatc acattgtga ccgaagttc gcccaaatg 
      cggacaaat ggtcaatta aggccaaaa ataaaatta 
      caaaaaccg agtttgcaa gtatttacg acgtggtga 
      tgatctgta ccggacatt ataacactc caatcagtc 
      caagtttgt cgggtcaag aaaaattca aaggccacg 
      ttatgcaca gtcatcagt acagatcga acaaaccgt 
      ttcaaaatc aaagagttt tggtgatcg gtgctgggc 
      cttcaggcc tagacgttg ctttccaag ttgctgaaa 
      ttgctcaac aagtcgttc tcagctatg acatgacta 
      agaaagaag tcaaggggg aatatccct ccaacctgg 
      tcaaaaaac cccaagtcc ttcgagtca aagacaagg 
      aacatgttg agtttgttg acggttctt gttgttcct 
      tcgacacga ttatatact gcacagggt acagatata 
      acttcccgt ttctccacc atgactgtg gagtgtccg 
      taggagatt tccatgtta gaccacttt acaaacact 
      tgattcata tcgaaaaac ccacaatgt gtttcattg 
      gtattccat attatgttg gtgctttcc aaatgtttg 
      acattcagg ctcgcttct actgccagt acctcaacg 
      gttcaatgt cgctgccta ccaaagaaa tgatgtaca 
      aagacaccg aagaagatg tagttaaac gaaaaaata 
      aaggatatt ccgaaaagc agatgcact tgttgggtc 
      acgatcaac aaacgtatt tcgaagagt tggcaagca 
      ctgcaaaaa ttactccaa tattgcccg ttatttgca 
      agatatgga gcgatagtg acaatagtt tttacaaag 
      atctcaaaa attttagaa agtacaagt acagaatcc 
      tcaataata acacatttg tttccgaac gctacatcg 
      aataatgga gaaataatg aaaatgtag taaaaacaa 
      gtaatatga ataaaagtg tctatttct t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY