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Gene information
General information
TC010101 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 8514172..8529798
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010101
    iBeetle screen iB_01649
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010101 RA
      MASHQPYTG DILHLNVGG KRFSTSKQT LTLIPDTFF 
TALLSGRIS SLRDEKGAI FIDRDPEIF SIILNYLRT 
REIDLHGTN IRTLRHEAE YYNIAPLVK RLILCEELT 
QSTCGDVLF YGYLPPPYV PIQDAPNPQ YVEATAVHS 
VRPGSSIRL PDGENSGEN GGPHWPHPP QIPPNQVIT 
TPYSRTQGH SRNSSMELR VSATYSRSS QDLRGLASR 
GHSRAASLD LRHVRNSSA DLNKLFRND IGLVFGNSG 
STWADPLRV QIIKAHHNW IVVGYAHFI VSYRLKDSS 
GWQQVFTSP HIEQNIERI AINAKMGSG ENSKMVAIS 
YGSQVRLWG ISDNGEKTN IGIFNLHVP VEYLFFIGS 
QLVALSSSG KLGVWHAMT QHWQTQDVM RIASFDTAG 
SILLLGCNN GCIYYIDMQ KFPLRMKDN DLLVTELYR 
DPNNDPVTA ISVYLTPKT NLCGNWIEI AYGTTSGAV 
RVIVQHPET VGHGPQLFQ TFTVHQSPV TKVTLSEKY 
LVSVCCEYN HVRSWRVTR FRGMLSTQP GSTPEASFK 
IVSLEAVEP SISHATSND CGPFGEQDD EQVFIQKIV 
PDADQLFVR LASNGRRVC VIKAVDGST VSAFCVHEC 
EGSSRMGSR PRRFIFTGH SNGSIQMWD LTTALDLAY 
KTDLADKTN GGPSPEELL QMLDQCDLS NSLCSTPCM 
SPLPSIAAA ARLKSSNVA FLNQQDDRA SSSS
    CDS sequence
    • >TC010101 RA
      atggcatcg caccagcct tacacaggt gacatcctc 
cacttgaac gtcggcggt aaaagattt tccacatca 
aaacaaacc ctaactctc atccctgac actttcttt 
accgcacta ctcagcgga cgaatctcg agcttacgt 
gatgaaaaa ggtgcgatt ttcatcgac cgcgatcct 
gaaattttc tcaatcatt ttaaattat ctgcggacg 
cgtgaaatc gatttacat ggcacaaac atacggacg 
ttgcgccac gaagccgag tactacaat atagccccc 
ttggtgaag cggttgata ctttgcgag gaactgaca 
cagtcaact tgtggagat gtgctgttt tacggttat 
ctgccaccc ccttacgtt ccaattcag gatgcgcct 
aatccgcag tatgtggaa gccacagct gttcattcc 
gtacgtcct ggctccagt attcgattg ccggatggt 
gaaaacagt ggggaaaat gggggcccg cattggcca 
catcctcct caaattccc ccgaatcaa gtaatcacg 
acgccctat agcaggact caaggacat tcgcgtaat 
tcttcaatg gaattgaga gtttcggca acttacagt 
cgaagttct caggattta aggggactg gcgagtcgg 
ggacattct cgggccgct tctttagat ttaagacac 
gttaggaat tcgtcagcc gatttgaac aagttgttt 
aggaatgat attggtttg gttttcgga aattcgggg 
tctacgtgg gctgaccca ttacgtgtc caaatcatt 
aaagcccat cacaattgg attgttgta ggttacgct 
cattttata gttagttac agactgaaa gactccagt 
ggttggcaa caagttttc actagcccc cacatagag 
caaaacatt gaacgaatc gcaatcaat gcgaaaatg 
ggtagcggt gaaaactct aaaatggtt gcgatttcg 
tacggaagt caagttcgt ttgtgggga atttcagat 
aatggcgaa aaaacaaac attggaata tttaatttg 
catgttcct gtcgagtat ttatttttt atcggtagt 
caattagtt gcgttgtct tcatccggg aagttggga 
gtttggcat gcaatgact caacattgg caaacgcaa 
gatgtcatg agaatcgct tcctttgat acagcagga 
tcgattctt ctccttggt tgtaataac ggttgtatt 
tactacata gatatgcaa aaatttcct ttaagaatg 
aaagataac gatttgtta gttactgaa ctttatcgg 
gatccgaat aatgatcct gttacggca atttccgtt 
tatctaaca ccgaaaaca aatttgtgt ggcaattgg 
attgaaatc gcatacgga acgacttct ggcgcagtg 
cgcgtcatc gtccaacat ccggaaaca gtaggtcac 
ggccctcaa ctcttccag acgttcaca gtgcaccaa 
agtcccgtc actaaagtg accttatct gagaaatat 
ctcgtttcg gtctgttgc gagtacaat catgtgcgt 
tcttggcga gtgactcgc ttcagaggc atgttgtcg 
acgcagccg ggctccacc ccagaagcc tccttcaag 
atcgtctcg ttggaagca gtggagccg tcaataagt 
cacgccacc tccaacgat tgtggcccg ttcggcgaa 
caagacgac gaacaggtt ttcatccag aaaattgtt 
cctgatgcg gatcaactg tttgtccga ttggcgagc 
aacgggaga agagtttgt gtgattaaa gccgtagat 
ggcagcact gtcagtgcg ttctgtgtg catgagtgt 
gaaggctcg agtcgaatg ggttcgagg ccgaggcgg 
tttattttt acgggacat tcgaatgga tcgatacaa 
atgtgggat ttgacgacg gcgttggat ttggcgtat 
aaaacggat ttagctgat aagacgaac ggaggaccc 
tcacctgag gaattgttg caaatgttg gaccaatgt 
gatttgagt aatagtttg tgttcgacg ccttgtatg 
tcgcctctt ccttccatt gcagcagct gcgagactc 
aaatccagt aatgtggcg tttttgaat caacaggac 
gatagagcc tcaagttcc agttaa
    mRNA sequence
    • >TC010101 RA
      ttacgtcat ttatttatc gagtcaacc tgtcaaaat 
atttcacac gattccatt tgaacaata ataaaaact 
ttctacagc atgtcgtgc ctcaattag tgctctaaa 
tcatggcat cgcaccagc cttacacag gtgacatcc 
tccacttga acgtcggcg gtaaaagat tttccacat 
caaaacaaa ccctaactc tcatccctg acactttct 
ttaccgcac tactcagcg gacgaatct cgagcttac 
gtgatgaaa aaggtgcga ttttcatcg accgcgatc 
ctgaaattt tctcaatca ttttaaatt atctgcgga 
cgcgtgaaa tcgatttac atggcacaa acatacgga 
cgttgcgcc acgaagccg agtactaca atatagccc 
ccttggtga agcggttga tactttgcg aggaactga 
cacagtcaa cttgtggag atgtgctgt tttacggtt 
atctgccac ccccttacg ttccaattc aggatgcgc 
ctaatccgc agtatgtgg aagccacag ctgttcatt 
ccgtacgtc ctggctcca gtattcgat tgccggatg 
gtgaaaaca gtggggaaa atgggggcc cgcattggc 
cacatcctc ctcaaattc ccccgaatc aagtaatca 
cgacgccct atagcagga ctcaaggac attcgcgta 
attcttcaa tggaattga gagtttcgg caacttaca 
gtcgaagtt ctcaggatt taaggggac tggcgagtc 
ggggacatt ctcgggccg cttctttag atttaagac 
acgttagga attcgtcag ccgatttga acaagttgt 
ttaggaatg atattggtt tggttttcg gaaattcgg 
ggtctacgt gggctgacc cattacgtg tccaaatca 
ttaaagccc atcacaatt ggattgttg taggttacg 
ctcatttta tagttagtt acagactga aagactcca 
gtggttggc aacaagttt tcactagcc cccacatag 
agcaaaaca ttgaacgaa tcgcaatca atgcgaaaa 
tgggtagcg gtgaaaact ctaaaatgg ttgcgattt 
cgtacggaa gtcaagttc gtttgtggg gaatttcag 
ataatggcg aaaaaacaa acattggaa tatttaatt 
tgcatgttc ctgtcgagt atttatttt ttatcggta 
gtcaattag ttgcgttgt cttcatccg ggaagttgg 
gagtttggc atgcaatga ctcaacatt ggcaaacgc 
aagatgtca tgagaatcg cttcctttg atacagcag 
gatcgattc ttctccttg gttgtaata acggttgta 
tttactaca tagatatgc aaaaatttc ctttaagaa 
tgaaagata acgatttgt tagttactg aactttatc 
gggatccga ataatgatc ctgttacgg caatttccg 
tttatctaa caccgaaaa caaatttgt gtggcaatt 
ggattgaaa tcgcatacg gaacgactt ctggcgcag 
tgcgcgtca tcgtccaac atccggaaa cagtaggtc 
acggccctc aactcttcc agacgttca cagtgcacc 
aaagtcccg tcactaaag tgaccttat ctgagaaat 
atctcgttt cggtctgtt gcgagtaca atcatgtgc 
gttcttggc gagtgactc gcttcagag gcatgttgt 
cgacgcagc cgggctcca ccccagaag cctccttca 
agatcgtct cgttggaag cagtggagc cgtcaataa 
gtcacgcca cctccaacg attgtggcc cgttcggcg 
aacaagacg acgaacagg ttttcatcc agaaaattg 
ttcctgatg cggatcaac tgtttgtcc gattggcga 
gcaacggga gaagagttt gtgtgatta aagccgtag 
atggcagca ctgtcagtg cgttctgtg tgcatgagt 
gtgaaggct cgagtcgaa tgggttcga ggccgaggc 
ggtttattt ttacgggac attcgaatg gatcgatac 
aaatgtggg atttgacga cggcgttgg atttggcgt 
ataaaacgg atttagctg ataagacga acggaggac 
cctcacctg aggaattgt tgcaaatgt tggaccaat 
gtgatttga gtaatagtt tgtgttcga cgccttgta 
tgtcgcctc ttccttcca ttgcagcag ctgcgagac 
tcaaatcca gtaatgtgg cgtttttga atcaacagg 
acgatagag cctcaagtt ccagttaaa aattggggc 
acgagtgtg caataaaaa tcttgcctt ggttatttt 
tatccttat tgttacttt tcagtggta ttagttttt 
aattaaatg aaatatcgg tgttattaa atggactct 
attgttgac tgaagcttt gctattttt tacaattgg 
caaatgctc ttgaaattg aattgtgat aaattttca 
tgtttcttg atgtagatc gtcctcttc atttattaa 
ataaaatac gaaaaa