| General information | |||
| TC010101 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 15181519..15197145 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC010101 | |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01649 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC010101 RA
MASHQPYTG DILHLNVGG KRFSTSKQT LTLIPDTFF TALLSGRIS SLRDEKGAI FIDRDPEIF SIILNYLRT REIDLHGTN IRTLRHEAE YYNIAPLVK RLILCEELT QSTCGDVLF YGYLPPPYV PIQDAPNPQ YVEATAVHS VRPGSSIRL PDGENSGEN GGPHWPHPP QIPPNQVIT TPYSRTQGH SRNSSMELR VSATYSRSS QDLRGLASR GHSRAASLD LRHVRNSSA DLNKLFRND IGLVFGNSG STWADPLRV QIIKAHHNW IVVGYAHFI VSYRLKDSS GWQQVFTSP HIEQNIERI AINAKMGSG ENSKMVAIS YGSQVRLWG ISDNGEKTN IGIFNLHVP VEYLFFIGS QLVALSSSG KLGVWHAMT QHWQTQDVM RIASFDTAG SILLLGCNN GCIYYIDMQ KFPLRMKDN DLLVTELYR DPNNDPVTA ISVYLTPKT NLCGNWIEI AYGTTSGAV RVIVQHPET VGHGPQLFQ TFTVHQSPV TKVTLSEKY LVSVCCEYN HVRSWRVTR FRGMLSTQP GSTPEASFK IVSLEAVEP SISHATSND CGPFGEQDD EQVFIQKIV PDADQLFVR LASNGRRVC VIKAVDGST VSAFCVHEC EGSSRMGSR PRRFIFTGH SNGSIQMWD LTTALDLAY KTDLADKTN GGPSPEELL QMLDQCDLS NSLCSTPCM SPLPSIAAA ARLKSSNVA FLNQQDDRA SSSS
- >TC010101 RA
atggcatcg caccagcct tacacaggt gacatcctc cacttgaac gtcggcggt aaaagattt tccacatca aaacaaacc ctaactctc atccctgac actttcttt accgcacta ctcagcgga cgaatctcg agcttacgt gatgaaaaa ggtgcgatt ttcatcgac cgcgatcct gaaattttc tcaatcatt ttaaattat ctgcggacg cgtgaaatc gatttacat ggcacaaac atacggacg ttgcgccac gaagccgag tactacaat atagccccc ttggtgaag cggttgata ctttgcgag gaactgaca cagtcaact tgtggagat gtgctgttt tacggttat ctgccaccc ccttacgtt ccaattcag gatgcgcct aatccgcag tatgtggaa gccacagct gttcattcc gtacgtcct ggctccagt attcgattg ccggatggt gaaaacagt ggggaaaat gggggcccg cattggcca catcctcct caaattccc ccgaatcaa gtaatcacg acgccctat agcaggact caaggacat tcgcgtaat tcttcaatg gaattgaga gtttcggca acttacagt cgaagttct caggattta aggggactg gcgagtcgg ggacattct cgggccgct tctttagat ttaagacac gttaggaat tcgtcagcc gatttgaac aagttgttt aggaatgat attggtttg gttttcgga aattcgggg tctacgtgg gctgaccca ttacgtgtc caaatcatt aaagcccat cacaattgg attgttgta ggttacgct cattttata gttagttac agactgaaa gactccagt ggttggcaa caagttttc actagcccc cacatagag caaaacatt gaacgaatc gcaatcaat gcgaaaatg ggtagcggt gaaaactct aaaatggtt gcgatttcg tacggaagt caagttcgt ttgtgggga atttcagat aatggcgaa aaaacaaac attggaata tttaatttg catgttcct gtcgagtat ttatttttt atcggtagt caattagtt gcgttgtct tcatccggg aagttggga gtttggcat gcaatgact caacattgg caaacgcaa gatgtcatg agaatcgct tcctttgat acagcagga tcgattctt ctccttggt tgtaataac ggttgtatt tactacata gatatgcaa aaatttcct ttaagaatg aaagataac gatttgtta gttactgaa ctttatcgg gatccgaat aatgatcct gttacggca atttccgtt tatctaaca ccgaaaaca aatttgtgt ggcaattgg attgaaatc gcatacgga acgacttct ggcgcagtg cgcgtcatc gtccaacat ccggaaaca gtaggtcac ggccctcaa ctcttccag acgttcaca gtgcaccaa agtcccgtc actaaagtg accttatct gagaaatat ctcgtttcg gtctgttgc gagtacaat catgtgcgt tcttggcga gtgactcgc ttcagaggc atgttgtcg acgcagccg ggctccacc ccagaagcc tccttcaag atcgtctcg ttggaagca gtggagccg tcaataagt cacgccacc tccaacgat tgtggcccg ttcggcgaa caagacgac gaacaggtt ttcatccag aaaattgtt cctgatgcg gatcaactg tttgtccga ttggcgagc aacgggaga agagtttgt gtgattaaa gccgtagat ggcagcact gtcagtgcg ttctgtgtg catgagtgt gaaggctcg agtcgaatg ggttcgagg ccgaggcgg tttattttt acgggacat tcgaatgga tcgatacaa atgtgggat ttgacgacg gcgttggat ttggcgtat aaaacggat ttagctgat aagacgaac ggaggaccc tcacctgag gaattgttg caaatgttg gaccaatgt gatttgagt aatagtttg tgttcgacg ccttgtatg tcgcctctt ccttccatt gcagcagct gcgagactc aaatccagt aatgtggcg tttttgaat caacaggac gatagagcc tcaagttcc agttaa
- >TC010101 RA
ttacgtcat ttatttatc gagtcaacc tgtcaaaat atttcacac gattccatt tgaacaata ataaaaact ttctacagc atgtcgtgc ctcaattag tgctctaaa tcatggcat cgcaccagc cttacacag gtgacatcc tccacttga acgtcggcg gtaaaagat tttccacat caaaacaaa ccctaactc tcatccctg acactttct ttaccgcac tactcagcg gacgaatct cgagcttac gtgatgaaa aaggtgcga ttttcatcg accgcgatc ctgaaattt tctcaatca ttttaaatt atctgcgga cgcgtgaaa tcgatttac atggcacaa acatacgga cgttgcgcc acgaagccg agtactaca atatagccc ccttggtga agcggttga tactttgcg aggaactga cacagtcaa cttgtggag atgtgctgt tttacggtt atctgccac ccccttacg ttccaattc aggatgcgc ctaatccgc agtatgtgg aagccacag ctgttcatt ccgtacgtc ctggctcca gtattcgat tgccggatg gtgaaaaca gtggggaaa atgggggcc cgcattggc cacatcctc ctcaaattc ccccgaatc aagtaatca cgacgccct atagcagga ctcaaggac attcgcgta attcttcaa tggaattga gagtttcgg caacttaca gtcgaagtt ctcaggatt taaggggac tggcgagtc ggggacatt ctcgggccg cttctttag atttaagac acgttagga attcgtcag ccgatttga acaagttgt ttaggaatg atattggtt tggttttcg gaaattcgg ggtctacgt gggctgacc cattacgtg tccaaatca ttaaagccc atcacaatt ggattgttg taggttacg ctcatttta tagttagtt acagactga aagactcca gtggttggc aacaagttt tcactagcc cccacatag agcaaaaca ttgaacgaa tcgcaatca atgcgaaaa tgggtagcg gtgaaaact ctaaaatgg ttgcgattt cgtacggaa gtcaagttc gtttgtggg gaatttcag ataatggcg aaaaaacaa acattggaa tatttaatt tgcatgttc ctgtcgagt atttatttt ttatcggta gtcaattag ttgcgttgt cttcatccg ggaagttgg gagtttggc atgcaatga ctcaacatt ggcaaacgc aagatgtca tgagaatcg cttcctttg atacagcag gatcgattc ttctccttg gttgtaata acggttgta tttactaca tagatatgc aaaaatttc ctttaagaa tgaaagata acgatttgt tagttactg aactttatc gggatccga ataatgatc ctgttacgg caatttccg tttatctaa caccgaaaa caaatttgt gtggcaatt ggattgaaa tcgcatacg gaacgactt ctggcgcag tgcgcgtca tcgtccaac atccggaaa cagtaggtc acggccctc aactcttcc agacgttca cagtgcacc aaagtcccg tcactaaag tgaccttat ctgagaaat atctcgttt cggtctgtt gcgagtaca atcatgtgc gttcttggc gagtgactc gcttcagag gcatgttgt cgacgcagc cgggctcca ccccagaag cctccttca agatcgtct cgttggaag cagtggagc cgtcaataa gtcacgcca cctccaacg attgtggcc cgttcggcg aacaagacg acgaacagg ttttcatcc agaaaattg ttcctgatg cggatcaac tgtttgtcc gattggcga gcaacggga gaagagttt gtgtgatta aagccgtag atggcagca ctgtcagtg cgttctgtg tgcatgagt gtgaaggct cgagtcgaa tgggttcga ggccgaggc ggtttattt ttacgggac attcgaatg gatcgatac aaatgtggg atttgacga cggcgttgg atttggcgt ataaaacgg atttagctg ataagacga acggaggac cctcacctg aggaattgt tgcaaatgt tggaccaat gtgatttga gtaatagtt tgtgttcga cgccttgta tgtcgcctc ttccttcca ttgcagcag ctgcgagac tcaaatcca gtaatgtgg cgtttttga atcaacagg acgatagag cctcaagtt ccagttaaa aattggggc acgagtgtg caataaaaa tcttgcctt ggttatttt tatccttat tgttacttt tcagtggta ttagttttt aattaaatg aaatatcgg tgttattaa atggactct attgttgac tgaagcttt gctattttt tacaattgg caaatgctc ttgaaattg aattgtgat aaattttca tgtttcttg atgtagatc gtcctcttc atttattaa ataaaatac gaaaaa
