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Gene information
General information
TC010101 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 8514172..8529798
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010101
    iBeetle screen iB_01649
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010101 RA
      MASHQPYTG DILHLNVGG KRFSTSKQT LTLIPDTFF 
      TALLSGRIS SLRDEKGAI FIDRDPEIF SIILNYLRT 
      REIDLHGTN IRTLRHEAE YYNIAPLVK RLILCEELT 
      QSTCGDVLF YGYLPPPYV PIQDAPNPQ YVEATAVHS 
      VRPGSSIRL PDGENSGEN GGPHWPHPP QIPPNQVIT 
      TPYSRTQGH SRNSSMELR VSATYSRSS QDLRGLASR 
      GHSRAASLD LRHVRNSSA DLNKLFRND IGLVFGNSG 
      STWADPLRV QIIKAHHNW IVVGYAHFI VSYRLKDSS 
      GWQQVFTSP HIEQNIERI AINAKMGSG ENSKMVAIS 
      YGSQVRLWG ISDNGEKTN IGIFNLHVP VEYLFFIGS 
      QLVALSSSG KLGVWHAMT QHWQTQDVM RIASFDTAG 
      SILLLGCNN GCIYYIDMQ KFPLRMKDN DLLVTELYR 
      DPNNDPVTA ISVYLTPKT NLCGNWIEI AYGTTSGAV 
      RVIVQHPET VGHGPQLFQ TFTVHQSPV TKVTLSEKY 
      LVSVCCEYN HVRSWRVTR FRGMLSTQP GSTPEASFK 
      IVSLEAVEP SISHATSND CGPFGEQDD EQVFIQKIV 
      PDADQLFVR LASNGRRVC VIKAVDGST VSAFCVHEC 
      EGSSRMGSR PRRFIFTGH SNGSIQMWD LTTALDLAY 
      KTDLADKTN GGPSPEELL QMLDQCDLS NSLCSTPCM 
      SPLPSIAAA ARLKSSNVA FLNQQDDRA SSSS
    CDS sequence
    • >TC010101 RA
      atggcatcg caccagcct tacacaggt gacatcctc 
      cacttgaac gtcggcggt aaaagattt tccacatca 
      aaacaaacc ctaactctc atccctgac actttcttt 
      accgcacta ctcagcgga cgaatctcg agcttacgt 
      gatgaaaaa ggtgcgatt ttcatcgac cgcgatcct 
      gaaattttc tcaatcatt ttaaattat ctgcggacg 
      cgtgaaatc gatttacat ggcacaaac atacggacg 
      ttgcgccac gaagccgag tactacaat atagccccc 
      ttggtgaag cggttgata ctttgcgag gaactgaca 
      cagtcaact tgtggagat gtgctgttt tacggttat 
      ctgccaccc ccttacgtt ccaattcag gatgcgcct 
      aatccgcag tatgtggaa gccacagct gttcattcc 
      gtacgtcct ggctccagt attcgattg ccggatggt 
      gaaaacagt ggggaaaat gggggcccg cattggcca 
      catcctcct caaattccc ccgaatcaa gtaatcacg 
      acgccctat agcaggact caaggacat tcgcgtaat 
      tcttcaatg gaattgaga gtttcggca acttacagt 
      cgaagttct caggattta aggggactg gcgagtcgg 
      ggacattct cgggccgct tctttagat ttaagacac 
      gttaggaat tcgtcagcc gatttgaac aagttgttt 
      aggaatgat attggtttg gttttcgga aattcgggg 
      tctacgtgg gctgaccca ttacgtgtc caaatcatt 
      aaagcccat cacaattgg attgttgta ggttacgct 
      cattttata gttagttac agactgaaa gactccagt 
      ggttggcaa caagttttc actagcccc cacatagag 
      caaaacatt gaacgaatc gcaatcaat gcgaaaatg 
      ggtagcggt gaaaactct aaaatggtt gcgatttcg 
      tacggaagt caagttcgt ttgtgggga atttcagat 
      aatggcgaa aaaacaaac attggaata tttaatttg 
      catgttcct gtcgagtat ttatttttt atcggtagt 
      caattagtt gcgttgtct tcatccggg aagttggga 
      gtttggcat gcaatgact caacattgg caaacgcaa 
      gatgtcatg agaatcgct tcctttgat acagcagga 
      tcgattctt ctccttggt tgtaataac ggttgtatt 
      tactacata gatatgcaa aaatttcct ttaagaatg 
      aaagataac gatttgtta gttactgaa ctttatcgg 
      gatccgaat aatgatcct gttacggca atttccgtt 
      tatctaaca ccgaaaaca aatttgtgt ggcaattgg 
      attgaaatc gcatacgga acgacttct ggcgcagtg 
      cgcgtcatc gtccaacat ccggaaaca gtaggtcac 
      ggccctcaa ctcttccag acgttcaca gtgcaccaa 
      agtcccgtc actaaagtg accttatct gagaaatat 
      ctcgtttcg gtctgttgc gagtacaat catgtgcgt 
      tcttggcga gtgactcgc ttcagaggc atgttgtcg 
      acgcagccg ggctccacc ccagaagcc tccttcaag 
      atcgtctcg ttggaagca gtggagccg tcaataagt 
      cacgccacc tccaacgat tgtggcccg ttcggcgaa 
      caagacgac gaacaggtt ttcatccag aaaattgtt 
      cctgatgcg gatcaactg tttgtccga ttggcgagc 
      aacgggaga agagtttgt gtgattaaa gccgtagat 
      ggcagcact gtcagtgcg ttctgtgtg catgagtgt 
      gaaggctcg agtcgaatg ggttcgagg ccgaggcgg 
      tttattttt acgggacat tcgaatgga tcgatacaa 
      atgtgggat ttgacgacg gcgttggat ttggcgtat 
      aaaacggat ttagctgat aagacgaac ggaggaccc 
      tcacctgag gaattgttg caaatgttg gaccaatgt 
      gatttgagt aatagtttg tgttcgacg ccttgtatg 
      tcgcctctt ccttccatt gcagcagct gcgagactc 
      aaatccagt aatgtggcg tttttgaat caacaggac 
      gatagagcc tcaagttcc agttaa
    mRNA sequence
    • >TC010101 RA
      ttacgtcat ttatttatc gagtcaacc tgtcaaaat 
      atttcacac gattccatt tgaacaata ataaaaact 
      ttctacagc atgtcgtgc ctcaattag tgctctaaa 
      tcatggcat cgcaccagc cttacacag gtgacatcc 
      tccacttga acgtcggcg gtaaaagat tttccacat 
      caaaacaaa ccctaactc tcatccctg acactttct 
      ttaccgcac tactcagcg gacgaatct cgagcttac 
      gtgatgaaa aaggtgcga ttttcatcg accgcgatc 
      ctgaaattt tctcaatca ttttaaatt atctgcgga 
      cgcgtgaaa tcgatttac atggcacaa acatacgga 
      cgttgcgcc acgaagccg agtactaca atatagccc 
      ccttggtga agcggttga tactttgcg aggaactga 
      cacagtcaa cttgtggag atgtgctgt tttacggtt 
      atctgccac ccccttacg ttccaattc aggatgcgc 
      ctaatccgc agtatgtgg aagccacag ctgttcatt 
      ccgtacgtc ctggctcca gtattcgat tgccggatg 
      gtgaaaaca gtggggaaa atgggggcc cgcattggc 
      cacatcctc ctcaaattc ccccgaatc aagtaatca 
      cgacgccct atagcagga ctcaaggac attcgcgta 
      attcttcaa tggaattga gagtttcgg caacttaca 
      gtcgaagtt ctcaggatt taaggggac tggcgagtc 
      ggggacatt ctcgggccg cttctttag atttaagac 
      acgttagga attcgtcag ccgatttga acaagttgt 
      ttaggaatg atattggtt tggttttcg gaaattcgg 
      ggtctacgt gggctgacc cattacgtg tccaaatca 
      ttaaagccc atcacaatt ggattgttg taggttacg 
      ctcatttta tagttagtt acagactga aagactcca 
      gtggttggc aacaagttt tcactagcc cccacatag 
      agcaaaaca ttgaacgaa tcgcaatca atgcgaaaa 
      tgggtagcg gtgaaaact ctaaaatgg ttgcgattt 
      cgtacggaa gtcaagttc gtttgtggg gaatttcag 
      ataatggcg aaaaaacaa acattggaa tatttaatt 
      tgcatgttc ctgtcgagt atttatttt ttatcggta 
      gtcaattag ttgcgttgt cttcatccg ggaagttgg 
      gagtttggc atgcaatga ctcaacatt ggcaaacgc 
      aagatgtca tgagaatcg cttcctttg atacagcag 
      gatcgattc ttctccttg gttgtaata acggttgta 
      tttactaca tagatatgc aaaaatttc ctttaagaa 
      tgaaagata acgatttgt tagttactg aactttatc 
      gggatccga ataatgatc ctgttacgg caatttccg 
      tttatctaa caccgaaaa caaatttgt gtggcaatt 
      ggattgaaa tcgcatacg gaacgactt ctggcgcag 
      tgcgcgtca tcgtccaac atccggaaa cagtaggtc 
      acggccctc aactcttcc agacgttca cagtgcacc 
      aaagtcccg tcactaaag tgaccttat ctgagaaat 
      atctcgttt cggtctgtt gcgagtaca atcatgtgc 
      gttcttggc gagtgactc gcttcagag gcatgttgt 
      cgacgcagc cgggctcca ccccagaag cctccttca 
      agatcgtct cgttggaag cagtggagc cgtcaataa 
      gtcacgcca cctccaacg attgtggcc cgttcggcg 
      aacaagacg acgaacagg ttttcatcc agaaaattg 
      ttcctgatg cggatcaac tgtttgtcc gattggcga 
      gcaacggga gaagagttt gtgtgatta aagccgtag 
      atggcagca ctgtcagtg cgttctgtg tgcatgagt 
      gtgaaggct cgagtcgaa tgggttcga ggccgaggc 
      ggtttattt ttacgggac attcgaatg gatcgatac 
      aaatgtggg atttgacga cggcgttgg atttggcgt 
      ataaaacgg atttagctg ataagacga acggaggac 
      cctcacctg aggaattgt tgcaaatgt tggaccaat 
      gtgatttga gtaatagtt tgtgttcga cgccttgta 
      tgtcgcctc ttccttcca ttgcagcag ctgcgagac 
      tcaaatcca gtaatgtgg cgtttttga atcaacagg 
      acgatagag cctcaagtt ccagttaaa aattggggc 
      acgagtgtg caataaaaa tcttgcctt ggttatttt 
      tatccttat tgttacttt tcagtggta ttagttttt 
      aattaaatg aaatatcgg tgttattaa atggactct 
      attgttgac tgaagcttt gctattttt tacaattgg 
      caaatgctc ttgaaattg aattgtgat aaattttca 
      tgtttcttg atgtagatc gtcctcttc atttattaa 
      ataaaatac gaaaaa