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Gene information
General information
TC010098 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 8581192..8585377
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010098
    iBeetle screen iB_01648
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010098 RA
      MAMNQENFN AEKFVQDLF EEGTKRACD ETVQRFNEN 
      TKLPEFYDE ELYKKGQKF FIDNIFALL YGKFLGLLS 
      VLSIPSIVR ILIYTKMSG SPLTAYRRY LSTVFHMVI 
      WYQSDFTPG SKLWQSVIE VKDKHNSVS KRCCNAGLG 
      RINQKDMAL TQFGFMGYA LAFRDKIGL HDAKEEELK 
      ALVHVWRVV GYVMGIEDR FNICRDSVE ETSEICRIL 
      ADQIFRPAI ERKDKDFIE MATHLSDGM WAMNPTLNA 
      KIYLNLVYR LLQSPNNNN QSNSFLIEL NFFETGLYY 
      FLILVIWSL QFNIFRIYH NYTQYVSLW LMDVFPFLA 
      YYKFGYANS HVKVLQGNL KES
    CDS sequence
    • >TC010098 RA
      atggcaatg aatcaagaa aattttaat gccgaaaag 
      tttgtgcaa gacttattc gaagagggg acaaagagg 
      gcgtgcgac gaaacggtg caaagattc aacgaaaac 
      acaaaattg cccgaattt tacgacgaa gaattgtac 
      aaaaagggt cagaaattt ttcatcgac aacattttt 
      gccctcctt tacggaaaa tttctgggc cttctctca 
      gttctctca attccttcc atcgttcgt attttaatt 
      tatacaaaa atgtcggga tcgccttta acagcctac 
      agaagatat ctttccact gtgtttcat atggttatt 
      tggtaccaa agcgacttt actccaggg tcaaaactt 
      tggcaatcg gtgattgag gtaaaagac aaacacaac 
      tcagtgagc aaacgctgc tgcaatgct ggtttgggc 
      cgaatcaat caaaaagac atggctttg acgcagttt 
      ggtttcatg ggctatgcg ttggctttc agggataaa 
      ataggcctc catgatgct aaagaggaa gagttgaag 
      gctcttgtg catgtgtgg agggtcgta ggatacgta 
      atggggatc gaagatcgg tttaacatt tgtcgagat 
      tcggttgaa gaaacgagc gaaatttgc cgcattttg 
      gctgaccaa atcttccga cctgctatt gagagaaaa 
      gataaagac tttattgag atggcgaca catctatcg 
      gacggcatg tgggcaatg aaccccact ttgaacgcc 
      aaaatttac ctaaattta gtttaccgc ttactgcaa 
      tcaccgaat aacaataat cagagcaac agctttcta 
      atcgagttg aattttttt gaaaccggt ctttactat 
      ttcctgatt ttggtaatt tggagcttg cagttcaat 
      atattcaga atttatcac aactacact cagtacgtg 
      tccttgtgg ttaatggac gtgttcccg tttttggct 
      tactataaa ttcggttat gccaactct cacgttaaa 
      gttttgcag gggaatttg aaagaaagt tga
    mRNA sequence
    • >TC010098 RA
      ataattgtc ttcggagtt ctagggcgg aacatctca 
      ttttaaggt aatcttgag tgaataggc attacaaat 
      tttatttga ataatctga tgaatcgtc tcaatactc 
      gtttcaatg tccgcaaaa cgcacaact taattattt 
      tcaccttta ttctcacta aaaccttgt cccgtttga 
      tatttttca tcacaccgt gtgcctgtc agctttcac 
      tttccttcc aacttgaag cagactgaa taatccact 
      gagaagaaa gttgcgagt tgaaaaata tcggagaaa 
      accatacat acgtagttt taacagcaa ttaaatcga 
      aattttcca aagaaaatc acgtttaac acgataaag 
      cgagtgcta aagtggtgg tacgcaatg ttttgtgaa 
      agagccatt gcacaagta ggtttacac cgactttcc 
      ttacatgtt tataagcac accgacttg gaaaacctg 
      taaaaagca tacgagcta gcagtctga acgcagcaa 
      aatggcaat gaatcaaga aaattttaa tgccgaaaa 
      gtttgtgca agacttatt cgaagaggg gacaaagag 
      ggcgtgcga cgaaacggt gcaaagatt caacgaaaa 
      cacaaaatt gcccgaatt ttacgacga agaattgta 
      caaaaaggg tcagaaatt tttcatcga caacatttt 
      tgccctcct ttacggaaa atttctggg ccttctctc 
      agttctctc aattccttc catcgttcg tattttaat 
      ttatacaaa aatgtcggg atcgccttt aacagccta 
      cagaagata tctttccac tgtgtttca tatggttat 
      ttggtacca aagcgactt tactccagg gtcaaaact 
      ttggcaatc ggtgattga ggtaaaaga caaacacaa 
      ctcagtgag caaacgctg ctgcaatgc tggtttggg 
      ccgaatcaa tcaaaaaga catggcttt gacgcagtt 
      tggtttcat gggctatgc gttggcttt cagggataa 
      aataggcct ccatgatgc taaagagga agagttgaa 
      ggctcttgt gcatgtgtg gagggtcgt aggatacgt 
      aatggggat cgaagatcg gtttaacat ttgtcgaga 
      ttcggttga agaaacgag cgaaatttg ccgcatttt 
      ggctgacca aatcttccg acctgctat tgagagaaa 
      agataaaga ctttattga gatggcgac acatctatc 
      ggacggcat gtgggcaat gaaccccac tttgaacgc 
      caaaattta cctaaattt agtttaccg cttactgca 
      atcaccgaa taacaataa tcagagcaa cagctttct 
      aatcgagtt gaatttttt tgaaaccgg tctttacta 
      tttcctgat tttggtaat ttggagctt gcagttcaa 
      tatattcag aatttatca caactacac tcagtacgt 
      gtccttgtg gttaatgga cgtgttccc gtttttggc 
      ttactataa attcggtta tgccaactc tcacgttaa 
      agttttgca ggggaattt gaaagaaag ttgaacttt 
      tcgagtttt tatttctta aaattaata tatacagct 
      ttaactacg ttagaatcg ttttaaaaa taaataatt 
      tgaataaat