| General information | |||
| TC033822
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 9736211..9742361 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC033822 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01646 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 3 (16 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
- >TC033822 RA
MKVILLLII YFRSRVMAL NYHLFPKNF IFGTATAAY QVEGAWNED GKGESIWDR VLHEYPDWV VDKTNGEIA CDSYHKYKE DVQLLKSLG IHFYRFSIS WSRILPTGK ADQINQAGI NYYNNLINE LLNNGIQPY VTMFHWDLP QALQDEGGW VERKIVNYF VDYAKVLFD HFGDRVKSW LTFNEVLQF CEGGYSLGE FAPFVQKPG VGGYLCGHH VLLAHGATY RLYENYYKE KQNGKIGIA VDCAWYEPD NPNWDKDQK ISEITMQMT CGWFLNPII FGNYPEIMI EMVRQNSLA KSRLPEFTP NEQKLLGGA VDFIGLNHY STFKVYPAT KKLYNQPSL FSDIGGILY HDASWPETA FFYFKIVPW GIRKVLVWI KNHYNNPPV LITENGFGD DGRLDDYDR ADYFRQYLY EILKAIQED SCNVTGYTA WSLMDTFEW MSGYMVRFG LYHIYPHIN NYPQPFLLK CKMKPIVLL LICASAFAL ADNPDYHFP DDFVFGAAT AAYQVEGAW NEDGKGENI WDRGIHEHQ DWVADKSNG DIACDSYHK YKEDVQLLK NLGVNFYRF SVSWSRILP TGKTDQINQ AGIDYYNNL INELLANGI EPYVTMFHW DLPQPLQDE GGWPERKTA DYFVDYAKV LFENFGDRV KHWMTFNEI MQICEAGYS GGSFAPFIK NPGIGGYEC THTVLLAHG RTYRLYDSD FRGQQQGQI GIAVDSYWH EPNYPDRET DQQASEIDM EMNYGWFVN PFVNGNYPD VMIERVKKT SIAEGYNKS RLPEFTPEE QEMMKGTYD FIGLNHYSS DKVYLADDG AGDHPSHWA DTGVIGYQD ASWPGSASD WLKVVPWGI NKLLVWIKN HYNNPPILV TENGFSDYG QLDDYDRAN YYKDYLYEI LKAIHEENC NVIGYTAWS LMDNFEWMA GYTQRFGLH YVDFEDPER PRTRKLSSY VYNNIVTTR HIDWDYYPD WPPTQDKTQ
- >TC033822 RA
atgaaggta attttacta ctaattatt tacttcaga agtagagtt atggcactt aattatcac ttatttccc aaaaatttt atttttggc accgccact gcggcctat caagtcgaa ggtgcttgg aatgaagat ggtaaaggc gaaagcatc tgggaccga gtccttcac gagtatcca gactgggtg gtagacaaa accaatgga gagatcgct tgtgactcg taccacaaa tacaaggaa gacgtccaa cttctcaaa agtctgggc atacacttc taccgtttt tccatatct tggtctaga attctcccg acgggaaaa gctgaccaa attaaccaa gcaggcatt aactactac aacaacctg attaacgag ttgctaaat aatgggatt cagccctat gtcacgatg ttccattgg gatttacct caagccctc caagacgaa ggcggttgg gttgaacga aaaattgtt aattatttt gttgactat gccaaagtt ttgtttgac cattttggc gatagagtc aagtcttgg ttaacattt aatgaggtt ttgcaattt tgcgaaggt ggttactct ctaggggaa ttcgctcct tttgtccaa aagccaggg gttggaggc tatttgtgt ggtcatcat gtgctccta gcacatggg gcgacatat cgtttgtat gaaaattat tacaaggaa aaacaaaat ggaaaaatt gggattgcc gttgattgc gcttggtat gaacccgac aatcctaac tgggacaaa gaccagaag atttcagaa atcacaatg caaatgacc tgtggatgg tttctcaac cccatcatt tttggcaat taccctgaa ataatgatt gaaatggtc agacagaac agccttgcc aaatcgcgt ttgccagaa ttcacaccc aacgaacaa aaattgcta ggaggagcc gttgatttt atcggtttg aatcactac tccactttt aaagtttac ccagctacg aaaaaactg tataatcaa ccttctctg ttcagtgat attggtggt atcttgtat cacgatgcc tcttggcca gagaccgcg tttttttat ttcaaaatt gttccttgg ggaattcga aaggtatta gtttggatc aaaaaccac tacaataac ccaccggtt ctgatcaca gaaaatggg tttggtgac gatggtcgg ctggatgat tacgacaga gcagattat tttcgacaa tacctttac gaaatattg aaggccatt caagaagat agttgtaat gtgactgga tatacggca tggagtttg atggataca tttgaatgg atgagtggt tacatggtt agattcggc ttgtatcat atatatccc catataaat aactatccc caaccattt cttctcaaa tgcaagatg aagccaatt gtgttgctg ctcatttgt gcaagtgct ttcgcactt gcagacaac cctgactac cacttcccc gatgacttc gtttttggc gctgcgact gccgcctac caagtcgaa ggtgcatgg aacgaagac gggaaaggt gagaacatc tgggacaga ggtatccac gagcaccaa gactgggtg gctgacaag tccaatgga gacatcgcc tgtgactcg taccacaag tacaaggaa gacgttcaa ctcttgaaa aatctggga gttaacttt taccgcttt tccgtatct tggtctaga attcttcca acggggaaa acagatcag attaatcaa gcagggatt gactattac aataacttg ataaatgag ttgttggct aatgggatc gagccttac gttacgatg ttccattgg gatttacca cagccgctg caagatgag ggcgggtgg ccggagaga aaaacggcc gattatttt gtcgattat gcgaaagtt ttgtttgag aattttggg gatagggtt aaacattgg atgactttt aatgagatt atgcagatt tgtgaagct gggtattct gggggtagt tttgctcct tttatcaaa aatccagga attgggggc tacgagtgt acccatacg gtacttcta gcccatggg agaacctac cggttgtac gacagtgac ttcagggga caacagcaa ggtcagatc gggattgcg gtcgattcc tattggcac gaacccaat tatcctgac cgggagact gaccaacaa gcttcagaa atcgatatg gagatgaat tatggatgg ttcgtcaat cccttcgtc aatggcaat tacccggac gttatgatc gaacgagtg aaaaagacc agcattgct gaaggatat aacaagtcc cgcctgcca gagtttacc ccagaagag caagaaatg atgaaggga acatacgat ttcattggt ttgaaccac tactcttct gataaagtt tacctagct gatgacggt gctggggac catccgtcc cattgggca gacaccggt gtcataggg taccaagac gcctcatgg ccagggtca gcctccgac tggcttaaa gtcgttcct tggggcatt aataaacta ctagtgtgg attaaaaac cactacaac aacccacca attctcgtc acggagaac ggattttcc gattatggt caactcgat gactatgac agagctaac tactacaaa gattatctc tatgagata ctaaaagct atccacgag gaaaattgt aacgttatt ggatacacc gcctggagt ttgatggac aatttcgaa tggatggct ggatataca caaagattc ggcttgcat tatgtcgat tttgaggat ccagagagg ccgagaacg aggaagtta tcttcgtat gtttataat aatattgtg acaacgagg catattgat tgggattat taccccgac tggccacca actcaagat aagacacaa taa
- >TC033822 RA
aatttattt taaaatgaa ggtaatttt actactaat tatttactt cagaagtag agttatggc acttaatta tcacttatt tcccaaaaa ttttatttt tggcaccgc cactgcggc ctatcaagt cgaaggtgc ttggaatga agatggtaa aggcgaaag catctggga ccgagtcct tcacgagta tccagactg ggtggtaga caaaaccaa tggagagat cgcttgtga ctcgtacca caaatacaa ggaagacgt ccaacttct caaaagtct gggcataca cttctaccg tttttccat atcttggtc tagaattct cccgacggg aaaagctga ccaaattaa ccaagcagg cattaacta ctacaacaa cctgattaa cgagttgct aaataatgg gattcagcc ctatgtcac gatgttcca ttgggattt acctcaagc cctccaaga cgaaggcgg ttgggttga acgaaaaat tgttaatta ttttgttga ctatgccaa agttttgtt tgaccattt tggcgatag agtcaagtc ttggttaac atttaatga ggttttgca attttgcga aggtggtta ctctctagg ggaattcgc tccttttgt ccaaaagcc aggggttgg aggctattt gtgtggtca tcatgtgct cctagcaca tggggcgac atatcgttt gtatgaaaa ttattacaa ggaaaaaca aaatggaaa aattgggat tgccgttga ttgcgcttg gtatgaacc cgacaatcc taactggga caaagacca gaagatttc agaaatcac aatgcaaat gacctgtgg atggtttct caaccccat catttttgg caattaccc tgaaataat gattgaaat ggtcagaca gaacagcct tgccaaatc gcgtttgcc agaattcac acccaacga acaaaaatt gctaggagg agccgttga ttttatcgg tttgaatca ctactccac ttttaaagt ttacccagc tacgaaaaa actgtataa tcaaccttc tctgttcag tgatattgg tggtatctt gtatcacga tgcctcttg gccagagac cgcgttttt ttatttcaa aattgttcc ttggggaat tcgaaaggt attagtttg gatcaaaaa ccactacaa taacccacc ggttctgat cacagaaaa tgggtttgg tgacgatgg tcggctgga tgattacga cagagcaga ttattttcg acaatacct ttacgaaat attgaaggc cattcaaga agatagttg taatgtgac tggatatac ggcatggag tttgatgga tacatttga atggatgag tggttacat ggttagatt cggcttgta tcatatata tccccatat aaataacta tccccaacc atttcttct caaatgcaa gatgaagcc aattgtgtt gctgctcat ttgtgcaag tgctttcgc acttgcaga caaccctga ctaccactt ccccgatga cttcgtttt tggcgctgc gactgccgc ctaccaagt cgaaggtgc atggaacga agacgggaa aggtgagaa catctggga cagaggtat ccacgagca ccaagactg ggtggctga caagtccaa tggagacat cgcctgtga ctcgtacca caagtacaa ggaagacgt tcaactctt gaaaaatct gggagttaa cttttaccg cttttccgt atcttggtc tagaattct tccaacggg gaaaacaga tcagattaa tcaagcagg gattgacta ttacaataa cttgataaa tgagttgtt ggctaatgg gatcgagcc ttacgttac gatgttcca ttgggattt accacagcc gctgcaaga tgagggcgg gtggccgga gagaaaaac ggccgatta ttttgtcga ttatgcgaa agttttgtt tgagaattt tggggatag ggttaaaca ttggatgac ttttaatga gattatgca gatttgtga agctgggta ttctggggg tagttttgc tccttttat caaaaatcc aggaattgg gggctacga gtgtaccca tacggtact tctagccca tgggagaac ctaccggtt gtacgacag tgacttcag gggacaaca gcaaggtca gatcgggat tgcggtcga ttcctattg gcacgaacc caattatcc tgaccggga gactgacca acaagcttc agaaatcga tatggagat gaattatgg atggttcgt caatccctt cgtcaatgg caattaccc ggacgttat gatcgaacg agtgaaaaa gaccagcat tgctgaagg atataacaa gtcccgcct gccagagtt taccccaga agagcaaga aatgatgaa gggaacata cgatttcat tggtttgaa ccactactc ttctgataa agtttacct agctgatga cggtgctgg ggaccatcc gtcccattg ggcagacac cggtgtcat agggtacca agacgcctc atggccagg gtcagcctc cgactggct taaagtcgt tccttgggg cattaataa actactagt gtggattaa aaaccacta caacaaccc accaattct cgtcacgga gaacggatt ttccgatta tggtcaact cgatgacta tgacagagc taactacta caaagatta tctctatga gatactaaa agctatcca cgaggaaaa ttgtaacgt tattggata caccgcctg gagtttgat ggacaattt cgaatggat ggctggata tacacaaag attcggctt gcattatgt cgattttga ggatccaga gaggccgag aacgaggaa gttatcttc gtatgttta taataatat tgtgacaac gaggcatat tgattggga ttattaccc cgactggcc accaactca agataagac acaataaat taatgtcca accatcaga cgtatttat ttcataaaa attcagtat aaaacattg gcacgaact gcatgagtt cttaagaag ttcaattac attataaaa caaaagact atgtatcac ggcaccaca aaaaatcat cagtcttca aaaccgtca agtctttac aacatacta gcgttttct tgaacacaa attctatcg tttccataa tttctgtgt aaacaccag tcagatctc tgccttagc aactatcca accgataat aaggcaata tggttaata agataaact gataaaaat aaaaactgt ggtcgaaaa ataaaagtt ttacctgcg a
