Status
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Gene information
General information
TC033822
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010052,TC010051
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9736211..9742361
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033822
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01646

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033822 RA
      MKVILLLII YFRSRVMAL NYHLFPKNF IFGTATAAY 
      QVEGAWNED GKGESIWDR VLHEYPDWV VDKTNGEIA 
      CDSYHKYKE DVQLLKSLG IHFYRFSIS WSRILPTGK 
      ADQINQAGI NYYNNLINE LLNNGIQPY VTMFHWDLP 
      QALQDEGGW VERKIVNYF VDYAKVLFD HFGDRVKSW 
      LTFNEVLQF CEGGYSLGE FAPFVQKPG VGGYLCGHH 
      VLLAHGATY RLYENYYKE KQNGKIGIA VDCAWYEPD 
      NPNWDKDQK ISEITMQMT CGWFLNPII FGNYPEIMI 
      EMVRQNSLA KSRLPEFTP NEQKLLGGA VDFIGLNHY 
      STFKVYPAT KKLYNQPSL FSDIGGILY HDASWPETA 
      FFYFKIVPW GIRKVLVWI KNHYNNPPV LITENGFGD 
      DGRLDDYDR ADYFRQYLY EILKAIQED SCNVTGYTA 
      WSLMDTFEW MSGYMVRFG LYHIYPHIN NYPQPFLLK 
      CKMKPIVLL LICASAFAL ADNPDYHFP DDFVFGAAT 
      AAYQVEGAW NEDGKGENI WDRGIHEHQ DWVADKSNG 
      DIACDSYHK YKEDVQLLK NLGVNFYRF SVSWSRILP 
      TGKTDQINQ AGIDYYNNL INELLANGI EPYVTMFHW 
      DLPQPLQDE GGWPERKTA DYFVDYAKV LFENFGDRV 
      KHWMTFNEI MQICEAGYS GGSFAPFIK NPGIGGYEC 
      THTVLLAHG RTYRLYDSD FRGQQQGQI GIAVDSYWH 
      EPNYPDRET DQQASEIDM EMNYGWFVN PFVNGNYPD 
      VMIERVKKT SIAEGYNKS RLPEFTPEE QEMMKGTYD 
      FIGLNHYSS DKVYLADDG AGDHPSHWA DTGVIGYQD 
      ASWPGSASD WLKVVPWGI NKLLVWIKN HYNNPPILV 
      TENGFSDYG QLDDYDRAN YYKDYLYEI LKAIHEENC 
      NVIGYTAWS LMDNFEWMA GYTQRFGLH YVDFEDPER 
      PRTRKLSSY VYNNIVTTR HIDWDYYPD WPPTQDKTQ
    CDS sequence
    • >TC033822 RA
      atgaaggta attttacta ctaattatt tacttcaga 
      agtagagtt atggcactt aattatcac ttatttccc 
      aaaaatttt atttttggc accgccact gcggcctat 
      caagtcgaa ggtgcttgg aatgaagat ggtaaaggc 
      gaaagcatc tgggaccga gtccttcac gagtatcca 
      gactgggtg gtagacaaa accaatgga gagatcgct 
      tgtgactcg taccacaaa tacaaggaa gacgtccaa 
      cttctcaaa agtctgggc atacacttc taccgtttt 
      tccatatct tggtctaga attctcccg acgggaaaa 
      gctgaccaa attaaccaa gcaggcatt aactactac 
      aacaacctg attaacgag ttgctaaat aatgggatt 
      cagccctat gtcacgatg ttccattgg gatttacct 
      caagccctc caagacgaa ggcggttgg gttgaacga 
      aaaattgtt aattatttt gttgactat gccaaagtt 
      ttgtttgac cattttggc gatagagtc aagtcttgg 
      ttaacattt aatgaggtt ttgcaattt tgcgaaggt 
      ggttactct ctaggggaa ttcgctcct tttgtccaa 
      aagccaggg gttggaggc tatttgtgt ggtcatcat 
      gtgctccta gcacatggg gcgacatat cgtttgtat 
      gaaaattat tacaaggaa aaacaaaat ggaaaaatt 
      gggattgcc gttgattgc gcttggtat gaacccgac 
      aatcctaac tgggacaaa gaccagaag atttcagaa 
      atcacaatg caaatgacc tgtggatgg tttctcaac 
      cccatcatt tttggcaat taccctgaa ataatgatt 
      gaaatggtc agacagaac agccttgcc aaatcgcgt 
      ttgccagaa ttcacaccc aacgaacaa aaattgcta 
      ggaggagcc gttgatttt atcggtttg aatcactac 
      tccactttt aaagtttac ccagctacg aaaaaactg 
      tataatcaa ccttctctg ttcagtgat attggtggt 
      atcttgtat cacgatgcc tcttggcca gagaccgcg 
      tttttttat ttcaaaatt gttccttgg ggaattcga 
      aaggtatta gtttggatc aaaaaccac tacaataac 
      ccaccggtt ctgatcaca gaaaatggg tttggtgac 
      gatggtcgg ctggatgat tacgacaga gcagattat 
      tttcgacaa tacctttac gaaatattg aaggccatt 
      caagaagat agttgtaat gtgactgga tatacggca 
      tggagtttg atggataca tttgaatgg atgagtggt 
      tacatggtt agattcggc ttgtatcat atatatccc 
      catataaat aactatccc caaccattt cttctcaaa 
      tgcaagatg aagccaatt gtgttgctg ctcatttgt 
      gcaagtgct ttcgcactt gcagacaac cctgactac 
      cacttcccc gatgacttc gtttttggc gctgcgact 
      gccgcctac caagtcgaa ggtgcatgg aacgaagac 
      gggaaaggt gagaacatc tgggacaga ggtatccac 
      gagcaccaa gactgggtg gctgacaag tccaatgga 
      gacatcgcc tgtgactcg taccacaag tacaaggaa 
      gacgttcaa ctcttgaaa aatctggga gttaacttt 
      taccgcttt tccgtatct tggtctaga attcttcca 
      acggggaaa acagatcag attaatcaa gcagggatt 
      gactattac aataacttg ataaatgag ttgttggct 
      aatgggatc gagccttac gttacgatg ttccattgg 
      gatttacca cagccgctg caagatgag ggcgggtgg 
      ccggagaga aaaacggcc gattatttt gtcgattat 
      gcgaaagtt ttgtttgag aattttggg gatagggtt 
      aaacattgg atgactttt aatgagatt atgcagatt 
      tgtgaagct gggtattct gggggtagt tttgctcct 
      tttatcaaa aatccagga attgggggc tacgagtgt 
      acccatacg gtacttcta gcccatggg agaacctac 
      cggttgtac gacagtgac ttcagggga caacagcaa 
      ggtcagatc gggattgcg gtcgattcc tattggcac 
      gaacccaat tatcctgac cgggagact gaccaacaa 
      gcttcagaa atcgatatg gagatgaat tatggatgg 
      ttcgtcaat cccttcgtc aatggcaat tacccggac 
      gttatgatc gaacgagtg aaaaagacc agcattgct 
      gaaggatat aacaagtcc cgcctgcca gagtttacc 
      ccagaagag caagaaatg atgaaggga acatacgat 
      ttcattggt ttgaaccac tactcttct gataaagtt 
      tacctagct gatgacggt gctggggac catccgtcc 
      cattgggca gacaccggt gtcataggg taccaagac 
      gcctcatgg ccagggtca gcctccgac tggcttaaa 
      gtcgttcct tggggcatt aataaacta ctagtgtgg 
      attaaaaac cactacaac aacccacca attctcgtc 
      acggagaac ggattttcc gattatggt caactcgat 
      gactatgac agagctaac tactacaaa gattatctc 
      tatgagata ctaaaagct atccacgag gaaaattgt 
      aacgttatt ggatacacc gcctggagt ttgatggac 
      aatttcgaa tggatggct ggatataca caaagattc 
      ggcttgcat tatgtcgat tttgaggat ccagagagg 
      ccgagaacg aggaagtta tcttcgtat gtttataat 
      aatattgtg acaacgagg catattgat tgggattat 
      taccccgac tggccacca actcaagat aagacacaa 
      taa
    mRNA sequence
    • >TC033822 RA
      aatttattt taaaatgaa ggtaatttt actactaat 
      tatttactt cagaagtag agttatggc acttaatta 
      tcacttatt tcccaaaaa ttttatttt tggcaccgc 
      cactgcggc ctatcaagt cgaaggtgc ttggaatga 
      agatggtaa aggcgaaag catctggga ccgagtcct 
      tcacgagta tccagactg ggtggtaga caaaaccaa 
      tggagagat cgcttgtga ctcgtacca caaatacaa 
      ggaagacgt ccaacttct caaaagtct gggcataca 
      cttctaccg tttttccat atcttggtc tagaattct 
      cccgacggg aaaagctga ccaaattaa ccaagcagg 
      cattaacta ctacaacaa cctgattaa cgagttgct 
      aaataatgg gattcagcc ctatgtcac gatgttcca 
      ttgggattt acctcaagc cctccaaga cgaaggcgg 
      ttgggttga acgaaaaat tgttaatta ttttgttga 
      ctatgccaa agttttgtt tgaccattt tggcgatag 
      agtcaagtc ttggttaac atttaatga ggttttgca 
      attttgcga aggtggtta ctctctagg ggaattcgc 
      tccttttgt ccaaaagcc aggggttgg aggctattt 
      gtgtggtca tcatgtgct cctagcaca tggggcgac 
      atatcgttt gtatgaaaa ttattacaa ggaaaaaca 
      aaatggaaa aattgggat tgccgttga ttgcgcttg 
      gtatgaacc cgacaatcc taactggga caaagacca 
      gaagatttc agaaatcac aatgcaaat gacctgtgg 
      atggtttct caaccccat catttttgg caattaccc 
      tgaaataat gattgaaat ggtcagaca gaacagcct 
      tgccaaatc gcgtttgcc agaattcac acccaacga 
      acaaaaatt gctaggagg agccgttga ttttatcgg 
      tttgaatca ctactccac ttttaaagt ttacccagc 
      tacgaaaaa actgtataa tcaaccttc tctgttcag 
      tgatattgg tggtatctt gtatcacga tgcctcttg 
      gccagagac cgcgttttt ttatttcaa aattgttcc 
      ttggggaat tcgaaaggt attagtttg gatcaaaaa 
      ccactacaa taacccacc ggttctgat cacagaaaa 
      tgggtttgg tgacgatgg tcggctgga tgattacga 
      cagagcaga ttattttcg acaatacct ttacgaaat 
      attgaaggc cattcaaga agatagttg taatgtgac 
      tggatatac ggcatggag tttgatgga tacatttga 
      atggatgag tggttacat ggttagatt cggcttgta 
      tcatatata tccccatat aaataacta tccccaacc 
      atttcttct caaatgcaa gatgaagcc aattgtgtt 
      gctgctcat ttgtgcaag tgctttcgc acttgcaga 
      caaccctga ctaccactt ccccgatga cttcgtttt 
      tggcgctgc gactgccgc ctaccaagt cgaaggtgc 
      atggaacga agacgggaa aggtgagaa catctggga 
      cagaggtat ccacgagca ccaagactg ggtggctga 
      caagtccaa tggagacat cgcctgtga ctcgtacca 
      caagtacaa ggaagacgt tcaactctt gaaaaatct 
      gggagttaa cttttaccg cttttccgt atcttggtc 
      tagaattct tccaacggg gaaaacaga tcagattaa 
      tcaagcagg gattgacta ttacaataa cttgataaa 
      tgagttgtt ggctaatgg gatcgagcc ttacgttac 
      gatgttcca ttgggattt accacagcc gctgcaaga 
      tgagggcgg gtggccgga gagaaaaac ggccgatta 
      ttttgtcga ttatgcgaa agttttgtt tgagaattt 
      tggggatag ggttaaaca ttggatgac ttttaatga 
      gattatgca gatttgtga agctgggta ttctggggg 
      tagttttgc tccttttat caaaaatcc aggaattgg 
      gggctacga gtgtaccca tacggtact tctagccca 
      tgggagaac ctaccggtt gtacgacag tgacttcag 
      gggacaaca gcaaggtca gatcgggat tgcggtcga 
      ttcctattg gcacgaacc caattatcc tgaccggga 
      gactgacca acaagcttc agaaatcga tatggagat 
      gaattatgg atggttcgt caatccctt cgtcaatgg 
      caattaccc ggacgttat gatcgaacg agtgaaaaa 
      gaccagcat tgctgaagg atataacaa gtcccgcct 
      gccagagtt taccccaga agagcaaga aatgatgaa 
      gggaacata cgatttcat tggtttgaa ccactactc 
      ttctgataa agtttacct agctgatga cggtgctgg 
      ggaccatcc gtcccattg ggcagacac cggtgtcat 
      agggtacca agacgcctc atggccagg gtcagcctc 
      cgactggct taaagtcgt tccttgggg cattaataa 
      actactagt gtggattaa aaaccacta caacaaccc 
      accaattct cgtcacgga gaacggatt ttccgatta 
      tggtcaact cgatgacta tgacagagc taactacta 
      caaagatta tctctatga gatactaaa agctatcca 
      cgaggaaaa ttgtaacgt tattggata caccgcctg 
      gagtttgat ggacaattt cgaatggat ggctggata 
      tacacaaag attcggctt gcattatgt cgattttga 
      ggatccaga gaggccgag aacgaggaa gttatcttc 
      gtatgttta taataatat tgtgacaac gaggcatat 
      tgattggga ttattaccc cgactggcc accaactca 
      agataagac acaataaat taatgtcca accatcaga 
      cgtatttat ttcataaaa attcagtat aaaacattg 
      gcacgaact gcatgagtt cttaagaag ttcaattac 
      attataaaa caaaagact atgtatcac ggcaccaca 
      aaaaatcat cagtcttca aaaccgtca agtctttac 
      aacatacta gcgttttct tgaacacaa attctatcg 
      tttccataa tttctgtgt aaacaccag tcagatctc 
      tgccttagc aactatcca accgataat aaggcaata 
      tggttaata agataaact gataaaaat aaaaactgt 
      ggtcgaaaa ataaaagtt ttacctgcg a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY