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Gene information
General information
TC010029 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 3378701..3391639
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010029
    iBeetle screen iB_01643
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010029 RA
      MYSMNYIGR FFSNFREFY NEINTATLT GAIDVIVVE 
      QEDGTYKCS PFHVRFGKL GVLRSREKV VDIEINGEP 
      ADIHMKLGE SGEAFFVEE LEDDENEIP DHLATSPIP 
      VNEFENIFT SQGRRRSFG EPPTLENEI NDYTKRRNT 
      ADNESSVKR ERDFLKRQI GLGNIGIGE NSTEDMSRS 
      MHSTTKESV EDLSKDNDI SDTIFKMDS LEMDTSRSE 
      SGPKPEEAS QGKDEHSSE SRSSKKKRR KTKKKNAPR 
      KSSSVNQIS PEVAEKNDS ATTDPSSIE SNSSEPELK 
      ELQNVEGSA QTPSNSRKI IDPDFHFFS DTELTSNVV 
      DSRADSPIQ VEAVQSDSE FEVKNRKGD NKDNGQSWE 
      WGGFPSVSP TNSPISEQA TKNDQFAIS NVFSFMKKS 
      HGQTEGIYL SDLNSVDPE VAALYFSTN KNKTTDADM 
      DCESGNGPS LAQSPNSAE GCKSIDSDF DEHIKGGRL 
      YSNEVSLSL CGWDPEPDA TRFDEHMIT FSDFCNNPM 
      LLEDPNLVV NIRGKYYNW KVAAPIISS IMVFNRPLL 
      QSSIDQLCN IHMPSNQES KDNKQEAKT SWWHWRRSK 
      TSREATPTV DTRKTVVEV KDTAIDIGD EHILQTDIS 
      VAQKIPKTE ETEEKDKEC AISISPQVC QLSEKCRKT 
      LRLSSKQIA SLNLRDGMN EIVFSVTTA YQGTTRCTC 
      HLYKWKWDD KIVISDIDG TITKSDVLG HILPIVGKD 
      WAQSGVAQL FNKIKGNGY KLLYLSARA IGQARITRE 
      YLRSIKQGN LTMPDGPIL LNPTSLITA FHREVIEKK 
      PEQFKISCM SDIKALFPS ESNPFYAGY GNRINDVWA 
      YRAVGIPIV RIFTINPKG ELKHELTQT FQSTYSTMT 
      YYVDQLFPP LIEAANDYS QFAYWRDPL PVVEELPEV 
      A
    • >TC010029 RB
      MYSMNYIGR FFSNFREFY NEINTATLT GAIDVIVVE 
      QEDGTYKCS PFHVRFGKL GVLRSREKV VDIEINGEP 
      ADIHMKLGE SGEAFFVEE LEDDENEIP DHLATSPIP 
      VNEFENIFT SQGRRRSFG EPPTLENEI NDYTKRRNT 
      ADNESSVKR ERDFLKRQI GLGNIGIGE NSTEDMSRS 
      MHSTTKESV EDLSKDNDI SDTIFKMDS LEMDTSRSE 
      SGPKPEEAS QGKDEHSSE SRSSKKKRR KTKKKNAPR 
      KSSSVNQIS PEVAEKNDS ATTDPSSIE SNSSEPELK 
      ELQNVEGSA QTPSNSRKI IDPDFHFFS DTELTSNVV 
      DSRADSPIQ VEAVQSDSE FEVKNRKGD NKDNGQSWE 
      WGGFPSVSP TNSPISEQA TKNDQFAIS NVFSFMKKS 
      HGQTEGIYL SDLNSVDPE VAALYFSTN KNKTTDADM 
      DCESGNGPS LAQSPNSAE GCKSIDSDF DEHIKGGRL 
      YSNEVSLSL CGWDPEPDA TRFDEHMIT FSDFCNNPM 
      LLEDPNLVV NIRGKYYNW KVAAPIISS IMVFNRPLL 
      QSSIDQLCN IHMPSNQES KDNKQEAKT SWWHWRRSK 
      TSREATPTV DTRKTVVEV KDTAIDIGD EHILQTDIS 
      VAQKIPKTE ETEEKDKEC AISISPQVC QLSEKCRKT 
      LRLSSKQIA SLNLRDGMN EIVFSVTTA YQGTTRCTC 
      HLYKWKWDD KIVISDIDG TITKSDVLG HILPIVGKD 
      WAQSGVAQL FNKIKGNGY KLLYLSARA IGQARITRE 
      YLRSIKQGN LTMPDGPIL LNPTSLITA FHREVIEKK 
      PEQFKISCM SDIKALFPS ESNPFYAGY GNRINDVWA 
      YRAVGIPIV RIFTINPKG ELKHELTQT FQSTYTGQS 
      LVVNDVFPP LLHKLRDND TSSDEIESD MSDSD
    CDS sequence
    • >TC010029 RA
      atgtattct atgaactat atcggacgc ttcttcagc 
      aactttcgc gagttttac aacgaaatc aacacagcg 
      acgctgacc ggagccatc gacgtcatc gtcgtggag 
      caggaggat gggacgtac aagtgctca ccgttccac 
      gtccgattt ggaaaactg ggagtcctc agaagtaga 
      gaaaaagtg gtggacatc gaaatcaac ggcgaaccc 
      gccgacatc cacatgaaa ctgggagag agcggggag 
      gctttcttc gtcgaggag ctcgaagat gatgagaac 
      gaaatcccg gaccatttg gccacgagc ccgatcccc 
      gtcaacgaa ttcgagaac atcttcacg tcgcaaggc 
      cggaggcgg agtttcggg gaacccccg actctcgaa 
      aacgaaatc aacgattac acaaaacgg cgcaatacc 
      gccgataac gagagcagc gtcaaacgc gagcgcgat 
      tttctcaaa cgacagatc ggtttgggg aacatcggg 
      atcggggag aacagcacg gaggacatg tccaggtcc 
      atgcactcc accaccaag gaaagcgtg gaggacttg 
      agcaaggac aacgacatt tcggacact atcttcaaa 
      atggacagt ctggagatg gacaccagt cggtcggag 
      agcgggcca aagcccgag gaagcgagt caggggaag 
      gacgagcac agcagcgag agtcggagt agtaagaaa 
      aagcggcgc aaaactaaa aagaaaaac gcgccgagg 
      aagtcgtcc agtgttaat cagatatcg cccgaagtg 
      gcggagaaa aatgactcg gcgacgacc gatcccagc 
      tcgattgag agcaattcg tcggagccc gagttgaag 
      gagttgcag aatgttgaa gggagtgct cagacgccg 
      tcgaatagt cgcaagatc atcgatccg gattttcat 
      ttcttcagc gatacagag ttgaccagt aatgtggtg 
      gattcgagg gccgattcg ccgattcag gtggaggca 
      gtgcagtcg gattcggag tttgaagtg aaaaatcgc 
      aaaggcgac aacaaagat aatggccaa agctgggaa 
      tggggcggc ttccccagc gtctcgccg acaaactcc 
      ccaatcagc gaacaagcc accaaaaac gaccagttc 
      gccatcagc aacgtcttc tccttcatg aaaaaatct 
      cacggccag acggaagga atctactta tcggacctc 
      aactcggtc gatccggaa gtggccgcc ctttatttc 
      tcgacaaac aaaaataaa actacagat gccgacatg 
      gattgtgaa tcaggaaat ggtccgtca ctggcccaa 
      agcccgaac agtgctgaa gggtgcaag tcgatcgat 
      tccgacttc gacgaacac atcaaaggg ggtcgcttg 
      tactctaac gaagtctcg ttgtcgctg tgcggctgg 
      gacccggaa ccggatgcc acgcgtttc gacgagcac 
      atgatcacc ttcagtgac ttctgcaac aaccccatg 
      ctgctggaa gacccgaat ttggtggtg aacatcaga 
      gggaaatac tacaactgg aaagtagca gcccctatt 
      atttcaagc atcatggtt ttcaacaga cctctcctg 
      cagtcatca attgatcaa ctgtgcaac atacatatg 
      ccctctaat caggagagc aaggacaat aaacaagaa 
      gctaagacg tcgtggtgg cattggcgc agatctaag 
      acttcaaga gaagcgacc ccgacggtt gatacgaga 
      aaaacggta gtcgaagtt aaggacaca gctatagat 
      atcggagat gagcacatc ctccaaacc gatatttca 
      gtagcccag aaaattccc aaaactgaa gagactgaa 
      gagaaggac aaggaatgt gccatctcg atctcgccc 
      caagtgtgc caactttcg gaaaagtgc aggaagacc 
      ttgcgcctc agctccaag caaattgcg agtttgaat 
      ttgcgcgac ggcatgaac gaaatcgtg ttcagtgta 
      acgacagcc tatcagggg acaacccgg tgcacctgc 
      cacttgtac aagtggaaa tgggacgat aagatcgtc 
      atttccgat atcgacggg accattacg aagtcggac 
      gtgttgggc cacatcctg cccattgtc gggaaagac 
      tgggcccaa tcgggcgtc gcccaattg tttaacaaa 
      attaagggc aacggttac aaactattg tacctcagc 
      gctcgagcg ataggccag gccagaatc acccgagag 
      taccttagg agtatcaaa caggggaac ttgaccatg 
      cccgatggt ccgattttg ctcaatccc acttcgttg 
      atcacggct ttccaccga gaagtgatt gagaagaag 
      cctgaacag ttcaagatt tcctgcatg agcgacatt 
      aaggcgctg tttccgtct gaatcaaat ccgttctat 
      gccggatac gggaatagg attaatgat gtgtgggcg 
      taccgggct gtgggaatc cctatagtt agaattttt 
      acaattaat cccaaaggg gagctcaag cacgagctc 
      acacaaacc ttccagtcg acatattcg accatgacg 
      tactacgta gaccagctg tttcctcca ttgatcgaa 
      gctgccaat gactactcg cagttcgcc tattggcgc 
      gatccactt ccggtcgtt gaagaactc ccggaagta 
      gcgtaa
    • >TC010029 RB
      atgtattct atgaactat atcggacgc ttcttcagc 
      aactttcgc gagttttac aacgaaatc aacacagcg 
      acgctgacc ggagccatc gacgtcatc gtcgtggag 
      caggaggat gggacgtac aagtgctca ccgttccac 
      gtccgattt ggaaaactg ggagtcctc agaagtaga 
      gaaaaagtg gtggacatc gaaatcaac ggcgaaccc 
      gccgacatc cacatgaaa ctgggagag agcggggag 
      gctttcttc gtcgaggag ctcgaagat gatgagaac 
      gaaatcccg gaccatttg gccacgagc ccgatcccc 
      gtcaacgaa ttcgagaac atcttcacg tcgcaaggc 
      cggaggcgg agtttcggg gaacccccg actctcgaa 
      aacgaaatc aacgattac acaaaacgg cgcaatacc 
      gccgataac gagagcagc gtcaaacgc gagcgcgat 
      tttctcaaa cgacagatc ggtttgggg aacatcggg 
      atcggggag aacagcacg gaggacatg tccaggtcc 
      atgcactcc accaccaag gaaagcgtg gaggacttg 
      agcaaggac aacgacatt tcggacact atcttcaaa 
      atggacagt ctggagatg gacaccagt cggtcggag 
      agcgggcca aagcccgag gaagcgagt caggggaag 
      gacgagcac agcagcgag agtcggagt agtaagaaa 
      aagcggcgc aaaactaaa aagaaaaac gcgccgagg 
      aagtcgtcc agtgttaat cagatatcg cccgaagtg 
      gcggagaaa aatgactcg gcgacgacc gatcccagc 
      tcgattgag agcaattcg tcggagccc gagttgaag 
      gagttgcag aatgttgaa gggagtgct cagacgccg 
      tcgaatagt cgcaagatc atcgatccg gattttcat 
      ttcttcagc gatacagag ttgaccagt aatgtggtg 
      gattcgagg gccgattcg ccgattcag gtggaggca 
      gtgcagtcg gattcggag tttgaagtg aaaaatcgc 
      aaaggcgac aacaaagat aatggccaa agctgggaa 
      tggggcggc ttccccagc gtctcgccg acaaactcc 
      ccaatcagc gaacaagcc accaaaaac gaccagttc 
      gccatcagc aacgtcttc tccttcatg aaaaaatct 
      cacggccag acggaagga atctactta tcggacctc 
      aactcggtc gatccggaa gtggccgcc ctttatttc 
      tcgacaaac aaaaataaa actacagat gccgacatg 
      gattgtgaa tcaggaaat ggtccgtca ctggcccaa 
      agcccgaac agtgctgaa gggtgcaag tcgatcgat 
      tccgacttc gacgaacac atcaaaggg ggtcgcttg 
      tactctaac gaagtctcg ttgtcgctg tgcggctgg 
      gacccggaa ccggatgcc acgcgtttc gacgagcac 
      atgatcacc ttcagtgac ttctgcaac aaccccatg 
      ctgctggaa gacccgaat ttggtggtg aacatcaga 
      gggaaatac tacaactgg aaagtagca gcccctatt 
      atttcaagc atcatggtt ttcaacaga cctctcctg 
      cagtcatca attgatcaa ctgtgcaac atacatatg 
      ccctctaat caggagagc aaggacaat aaacaagaa 
      gctaagacg tcgtggtgg cattggcgc agatctaag 
      acttcaaga gaagcgacc ccgacggtt gatacgaga 
      aaaacggta gtcgaagtt aaggacaca gctatagat 
      atcggagat gagcacatc ctccaaacc gatatttca 
      gtagcccag aaaattccc aaaactgaa gagactgaa 
      gagaaggac aaggaatgt gccatctcg atctcgccc 
      caagtgtgc caactttcg gaaaagtgc aggaagacc 
      ttgcgcctc agctccaag caaattgcg agtttgaat 
      ttgcgcgac ggcatgaac gaaatcgtg ttcagtgta 
      acgacagcc tatcagggg acaacccgg tgcacctgc 
      cacttgtac aagtggaaa tgggacgat aagatcgtc 
      atttccgat atcgacggg accattacg aagtcggac 
      gtgttgggc cacatcctg cccattgtc gggaaagac 
      tgggcccaa tcgggcgtc gcccaattg tttaacaaa 
      attaagggc aacggttac aaactattg tacctcagc 
      gctcgagcg ataggccag gccagaatc acccgagag 
      taccttagg agtatcaaa caggggaac ttgaccatg 
      cccgatggt ccgattttg ctcaatccc acttcgttg 
      atcacggct ttccaccga gaagtgatt gagaagaag 
      cctgaacag ttcaagatt tcctgcatg agcgacatt 
      aaggcgctg tttccgtct gaatcaaat ccgttctat 
      gccggatac gggaatagg attaatgat gtgtgggcg 
      taccgggct gtgggaatc cctatagtt agaattttt 
      acaattaat cccaaaggg gagctcaag cacgagctc 
      acacaaacc ttccagtcg acttacacg ggtcagtca 
      ctggtcgtc aatgacgtg tttccccca cttttgcat 
      aaactcaga gacaatgac actagtagt gacgaaata 
      gagtcagat atgagcgat agcgattaa
    mRNA sequence
    • >TC010029 RA
      tcccacttg tcaataatt tatctgtca gttgctaga 
      cgtagtcac aggtgaatg tagctgacc gttttgtga 
      atttatggt gcttgataa aagaaataa atcgtcaag 
      attcaatta gattagatt gctagcgca ggacgcgcg 
      cgccaggat aacaacaga cactataaa aatacgcgt 
      gttttgttt ttccactca ttatcgagt tggaatctg 
      tgtgaagtt caagtgctt gtgattaaa taaccaaaa 
      tgtattcta tgaactata tcggacgct tcttcagca 
      actttcgcg agttttaca acgaaatca acacagcga 
      cgctgaccg gagccatcg acgtcatcg tcgtggagc 
      aggaggatg ggacgtaca agtgctcac cgttccacg 
      tccgatttg gaaaactgg gagtcctca gaagtagag 
      aaaaagtgg tggacatcg aaatcaacg gcgaacccg 
      ccgacatcc acatgaaac tgggagaga gcggggagg 
      ctttcttcg tcgaggagc tcgaagatg atgagaacg 
      aaatcccgg accatttgg ccacgagcc cgatccccg 
      tcaacgaat tcgagaaca tcttcacgt cgcaaggcc 
      ggaggcgga gtttcgggg aacccccga ctctcgaaa 
      acgaaatca acgattaca caaaacggc gcaataccg 
      ccgataacg agagcagcg tcaaacgcg agcgcgatt 
      ttctcaaac gacagatcg gtttgggga acatcggga 
      tcggggaga acagcacgg aggacatgt ccaggtcca 
      tgcactcca ccaccaagg aaagcgtgg aggacttga 
      gcaaggaca acgacattt cggacacta tcttcaaaa 
      tggacagtc tggagatgg acaccagtc ggtcggaga 
      gcgggccaa agcccgagg aagcgagtc aggggaagg 
      acgagcaca gcagcgaga gtcggagta gtaagaaaa 
      agcggcgca aaactaaaa agaaaaacg cgccgagga 
      agtcgtcca gtgttaatc agatatcgc ccgaagtgg 
      cggagaaaa atgactcgg cgacgaccg atcccagct 
      cgattgaga gcaattcgt cggagcccg agttgaagg 
      agttgcaga atgttgaag ggagtgctc agacgccgt 
      cgaatagtc gcaagatca tcgatccgg attttcatt 
      tcttcagcg atacagagt tgaccagta atgtggtgg 
      attcgaggg ccgattcgc cgattcagg tggaggcag 
      tgcagtcgg attcggagt ttgaagtga aaaatcgca 
      aaggcgaca acaaagata atggccaaa gctgggaat 
      ggggcggct tccccagcg tctcgccga caaactccc 
      caatcagcg aacaagcca ccaaaaacg accagttcg 
      ccatcagca acgtcttct ccttcatga aaaaatctc 
      acggccaga cggaaggaa tctacttat cggacctca 
      actcggtcg atccggaag tggccgccc tttatttct 
      cgacaaaca aaaataaaa ctacagatg ccgacatgg 
      attgtgaat caggaaatg gtccgtcac tggcccaaa 
      gcccgaaca gtgctgaag ggtgcaagt cgatcgatt 
      ccgacttcg acgaacaca tcaaagggg gtcgcttgt 
      actctaacg aagtctcgt tgtcgctgt gcggctggg 
      acccggaac cggatgcca cgcgtttcg acgagcaca 
      tgatcacct tcagtgact tctgcaaca accccatgc 
      tgctggaag acccgaatt tggtggtga acatcagag 
      ggaaatact acaactgga aagtagcag cccctatta 
      tttcaagca tcatggttt tcaacagac ctctcctgc 
      agtcatcaa ttgatcaac tgtgcaaca tacatatgc 
      cctctaatc aggagagca aggacaata aacaagaag 
      ctaagacgt cgtggtggc attggcgca gatctaaga 
      cttcaagag aagcgaccc cgacggttg atacgagaa 
      aaacggtag tcgaagtta aggacacag ctatagata 
      tcggagatg agcacatcc tccaaaccg atatttcag 
      tagcccaga aaattccca aaactgaag agactgaag 
      agaaggaca aggaatgtg ccatctcga tctcgcccc 
      aagtgtgcc aactttcgg aaaagtgca ggaagacct 
      tgcgcctca gctccaagc aaattgcga gtttgaatt 
      tgcgcgacg gcatgaacg aaatcgtgt tcagtgtaa 
      cgacagcct atcagggga caacccggt gcacctgcc 
      acttgtaca agtggaaat gggacgata agatcgtca 
      tttccgata tcgacggga ccattacga agtcggacg 
      tgttgggcc acatcctgc ccattgtcg ggaaagact 
      gggcccaat cgggcgtcg cccaattgt ttaacaaaa 
      ttaagggca acggttaca aactattgt acctcagcg 
      ctcgagcga taggccagg ccagaatca cccgagagt 
      accttagga gtatcaaac aggggaact tgaccatgc 
      ccgatggtc cgattttgc tcaatccca cttcgttga 
      tcacggctt tccaccgag aagtgattg agaagaagc 
      ctgaacagt tcaagattt cctgcatga gcgacatta 
      aggcgctgt ttccgtctg aatcaaatc cgttctatg 
      ccggatacg ggaatagga ttaatgatg tgtgggcgt 
      accgggctg tgggaatcc ctatagtta gaattttta 
      caattaatc ccaaagggg agctcaagc acgagctca 
      cacaaacct tccagtcga catattcga ccatgacgt 
      actacgtag accagctgt ttcctccat tgatcgaag 
      ctgccaatg actactcgc agttcgcct attggcgcg 
      atccacttc cggtcgttg aagaactcc cggaagtag 
      cgtaaaatt gaatttgtc tgcatttat cgattcagt 
      atttgttgt cgtgcgctt tcgatgtct ccattaccg 
      gacacagct tttagaaca aatcaagta gctgtcact 
      gttataaat tacaataat aacgataat gtgatatac 
      tgattacca acttattgt aaggctagg cttaaatac 
      tctacctgt taaacaaat tgtgcgttg ctttgttca 
      attttagta ctaaaaagt gattcacta ctttttata 
      ttgtgatat tttatttcg taagggata cactatttt 
      ttttgtttt ttttttaag ttattgcaa taaagttcc 
      aactgtata
    • >TC010029 RB
      tcccacttg tcaataatt tatctgtca gttgctaga 
      cgtagtcac aggtgaatg tagctgacc gttttgtga 
      atttatggt gcttgataa aagaaataa atcgtcaag 
      attcaatta gattagatt gctagcgca ggacgcgcg 
      cgccaggat aacaacaga cactataaa aatacgcgt 
      gttttgttt ttccactca ttatcgagt tggaatctg 
      tgtgaagtt caagtgctt gtgattaaa taaccaaaa 
      tgtattcta tgaactata tcggacgct tcttcagca 
      actttcgcg agttttaca acgaaatca acacagcga 
      cgctgaccg gagccatcg acgtcatcg tcgtggagc 
      aggaggatg ggacgtaca agtgctcac cgttccacg 
      tccgatttg gaaaactgg gagtcctca gaagtagag 
      aaaaagtgg tggacatcg aaatcaacg gcgaacccg 
      ccgacatcc acatgaaac tgggagaga gcggggagg 
      ctttcttcg tcgaggagc tcgaagatg atgagaacg 
      aaatcccgg accatttgg ccacgagcc cgatccccg 
      tcaacgaat tcgagaaca tcttcacgt cgcaaggcc 
      ggaggcgga gtttcgggg aacccccga ctctcgaaa 
      acgaaatca acgattaca caaaacggc gcaataccg 
      ccgataacg agagcagcg tcaaacgcg agcgcgatt 
      ttctcaaac gacagatcg gtttgggga acatcggga 
      tcggggaga acagcacgg aggacatgt ccaggtcca 
      tgcactcca ccaccaagg aaagcgtgg aggacttga 
      gcaaggaca acgacattt cggacacta tcttcaaaa 
      tggacagtc tggagatgg acaccagtc ggtcggaga 
      gcgggccaa agcccgagg aagcgagtc aggggaagg 
      acgagcaca gcagcgaga gtcggagta gtaagaaaa 
      agcggcgca aaactaaaa agaaaaacg cgccgagga 
      agtcgtcca gtgttaatc agatatcgc ccgaagtgg 
      cggagaaaa atgactcgg cgacgaccg atcccagct 
      cgattgaga gcaattcgt cggagcccg agttgaagg 
      agttgcaga atgttgaag ggagtgctc agacgccgt 
      cgaatagtc gcaagatca tcgatccgg attttcatt 
      tcttcagcg atacagagt tgaccagta atgtggtgg 
      attcgaggg ccgattcgc cgattcagg tggaggcag 
      tgcagtcgg attcggagt ttgaagtga aaaatcgca 
      aaggcgaca acaaagata atggccaaa gctgggaat 
      ggggcggct tccccagcg tctcgccga caaactccc 
      caatcagcg aacaagcca ccaaaaacg accagttcg 
      ccatcagca acgtcttct ccttcatga aaaaatctc 
      acggccaga cggaaggaa tctacttat cggacctca 
      actcggtcg atccggaag tggccgccc tttatttct 
      cgacaaaca aaaataaaa ctacagatg ccgacatgg 
      attgtgaat caggaaatg gtccgtcac tggcccaaa 
      gcccgaaca gtgctgaag ggtgcaagt cgatcgatt 
      ccgacttcg acgaacaca tcaaagggg gtcgcttgt 
      actctaacg aagtctcgt tgtcgctgt gcggctggg 
      acccggaac cggatgcca cgcgtttcg acgagcaca 
      tgatcacct tcagtgact tctgcaaca accccatgc 
      tgctggaag acccgaatt tggtggtga acatcagag 
      ggaaatact acaactgga aagtagcag cccctatta 
      tttcaagca tcatggttt tcaacagac ctctcctgc 
      agtcatcaa ttgatcaac tgtgcaaca tacatatgc 
      cctctaatc aggagagca aggacaata aacaagaag 
      ctaagacgt cgtggtggc attggcgca gatctaaga 
      cttcaagag aagcgaccc cgacggttg atacgagaa 
      aaacggtag tcgaagtta aggacacag ctatagata 
      tcggagatg agcacatcc tccaaaccg atatttcag 
      tagcccaga aaattccca aaactgaag agactgaag 
      agaaggaca aggaatgtg ccatctcga tctcgcccc 
      aagtgtgcc aactttcgg aaaagtgca ggaagacct 
      tgcgcctca gctccaagc aaattgcga gtttgaatt 
      tgcgcgacg gcatgaacg aaatcgtgt tcagtgtaa 
      cgacagcct atcagggga caacccggt gcacctgcc 
      acttgtaca agtggaaat gggacgata agatcgtca 
      tttccgata tcgacggga ccattacga agtcggacg 
      tgttgggcc acatcctgc ccattgtcg ggaaagact 
      gggcccaat cgggcgtcg cccaattgt ttaacaaaa 
      ttaagggca acggttaca aactattgt acctcagcg 
      ctcgagcga taggccagg ccagaatca cccgagagt 
      accttagga gtatcaaac aggggaact tgaccatgc 
      ccgatggtc cgattttgc tcaatccca cttcgttga 
      tcacggctt tccaccgag aagtgattg agaagaagc 
      ctgaacagt tcaagattt cctgcatga gcgacatta 
      aggcgctgt ttccgtctg aatcaaatc cgttctatg 
      ccggatacg ggaatagga ttaatgatg tgtgggcgt 
      accgggctg tgggaatcc ctatagtta gaattttta 
      caattaatc ccaaagggg agctcaagc acgagctca 
      cacaaacct tccagtcga cttacacgg gtcagtcac 
      tggtcgtca atgacgtgt ttcccccac ttttgcata 
      aactcagag acaatgaca ctagtagtg acgaaatag 
      agtcagata tgagcgata gcgattaat ttgtatttt 
      atcagtaca aacactctc atctagttc atgcaacta 
      acggtggta gcgcttatc attcatcct catgatcat 
      caggggctt atcgcgaac gcatccaat gataaaata 
      caaatgagt tcgcgttta atttattta tttattgtt 
      taaatgtta ttgtcttaa actccggcc ctctccgtg 
      gtttattat aacggtaag cttaagtca gtat