| General information | |||
| TC010029 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 10046048..10058986 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC010029 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01643 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
Days post injection
larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
Days post injection
- >TC010029 RA
MYSMNYIGR FFSNFREFY NEINTATLT GAIDVIVVE QEDGTYKCS PFHVRFGKL GVLRSREKV VDIEINGEP ADIHMKLGE SGEAFFVEE LEDDENEIP DHLATSPIP VNEFENIFT SQGRRRSFG EPPTLENEI NDYTKRRNT ADNESSVKR ERDFLKRQI GLGNIGIGE NSTEDMSRS MHSTTKESV EDLSKDNDI SDTIFKMDS LEMDTSRSE SGPKPEEAS QGKDEHSSE SRSSKKKRR KTKKKNAPR KSSSVNQIS PEVAEKNDS ATTDPSSIE SNSSEPELK ELQNVEGSA QTPSNSRKI IDPDFHFFS DTELTSNVV DSRADSPIQ VEAVQSDSE FEVKNRKGD NKDNGQSWE WGGFPSVSP TNSPISEQA TKNDQFAIS NVFSFMKKS HGQTEGIYL SDLNSVDPE VAALYFSTN KNKTTDADM DCESGNGPS LAQSPNSAE GCKSIDSDF DEHIKGGRL YSNEVSLSL CGWDPEPDA TRFDEHMIT FSDFCNNPM LLEDPNLVV NIRGKYYNW KVAAPIISS IMVFNRPLL QSSIDQLCN IHMPSNQES KDNKQEAKT SWWHWRRSK TSREATPTV DTRKTVVEV KDTAIDIGD EHILQTDIS VAQKIPKTE ETEEKDKEC AISISPQVC QLSEKCRKT LRLSSKQIA SLNLRDGMN EIVFSVTTA YQGTTRCTC HLYKWKWDD KIVISDIDG TITKSDVLG HILPIVGKD WAQSGVAQL FNKIKGNGY KLLYLSARA IGQARITRE YLRSIKQGN LTMPDGPIL LNPTSLITA FHREVIEKK PEQFKISCM SDIKALFPS ESNPFYAGY GNRINDVWA YRAVGIPIV RIFTINPKG ELKHELTQT FQSTYSTMT YYVDQLFPP LIEAANDYS QFAYWRDPL PVVEELPEV A
- >TC010029 RB
MYSMNYIGR FFSNFREFY NEINTATLT GAIDVIVVE QEDGTYKCS PFHVRFGKL GVLRSREKV VDIEINGEP ADIHMKLGE SGEAFFVEE LEDDENEIP DHLATSPIP VNEFENIFT SQGRRRSFG EPPTLENEI NDYTKRRNT ADNESSVKR ERDFLKRQI GLGNIGIGE NSTEDMSRS MHSTTKESV EDLSKDNDI SDTIFKMDS LEMDTSRSE SGPKPEEAS QGKDEHSSE SRSSKKKRR KTKKKNAPR KSSSVNQIS PEVAEKNDS ATTDPSSIE SNSSEPELK ELQNVEGSA QTPSNSRKI IDPDFHFFS DTELTSNVV DSRADSPIQ VEAVQSDSE FEVKNRKGD NKDNGQSWE WGGFPSVSP TNSPISEQA TKNDQFAIS NVFSFMKKS HGQTEGIYL SDLNSVDPE VAALYFSTN KNKTTDADM DCESGNGPS LAQSPNSAE GCKSIDSDF DEHIKGGRL YSNEVSLSL CGWDPEPDA TRFDEHMIT FSDFCNNPM LLEDPNLVV NIRGKYYNW KVAAPIISS IMVFNRPLL QSSIDQLCN IHMPSNQES KDNKQEAKT SWWHWRRSK TSREATPTV DTRKTVVEV KDTAIDIGD EHILQTDIS VAQKIPKTE ETEEKDKEC AISISPQVC QLSEKCRKT LRLSSKQIA SLNLRDGMN EIVFSVTTA YQGTTRCTC HLYKWKWDD KIVISDIDG TITKSDVLG HILPIVGKD WAQSGVAQL FNKIKGNGY KLLYLSARA IGQARITRE YLRSIKQGN LTMPDGPIL LNPTSLITA FHREVIEKK PEQFKISCM SDIKALFPS ESNPFYAGY GNRINDVWA YRAVGIPIV RIFTINPKG ELKHELTQT FQSTYTGQS LVVNDVFPP LLHKLRDND TSSDEIESD MSDSD
- >TC010029 RA
atgtattct atgaactat atcggacgc ttcttcagc aactttcgc gagttttac aacgaaatc aacacagcg acgctgacc ggagccatc gacgtcatc gtcgtggag caggaggat gggacgtac aagtgctca ccgttccac gtccgattt ggaaaactg ggagtcctc agaagtaga gaaaaagtg gtggacatc gaaatcaac ggcgaaccc gccgacatc cacatgaaa ctgggagag agcggggag gctttcttc gtcgaggag ctcgaagat gatgagaac gaaatcccg gaccatttg gccacgagc ccgatcccc gtcaacgaa ttcgagaac atcttcacg tcgcaaggc cggaggcgg agtttcggg gaacccccg actctcgaa aacgaaatc aacgattac acaaaacgg cgcaatacc gccgataac gagagcagc gtcaaacgc gagcgcgat tttctcaaa cgacagatc ggtttgggg aacatcggg atcggggag aacagcacg gaggacatg tccaggtcc atgcactcc accaccaag gaaagcgtg gaggacttg agcaaggac aacgacatt tcggacact atcttcaaa atggacagt ctggagatg gacaccagt cggtcggag agcgggcca aagcccgag gaagcgagt caggggaag gacgagcac agcagcgag agtcggagt agtaagaaa aagcggcgc aaaactaaa aagaaaaac gcgccgagg aagtcgtcc agtgttaat cagatatcg cccgaagtg gcggagaaa aatgactcg gcgacgacc gatcccagc tcgattgag agcaattcg tcggagccc gagttgaag gagttgcag aatgttgaa gggagtgct cagacgccg tcgaatagt cgcaagatc atcgatccg gattttcat ttcttcagc gatacagag ttgaccagt aatgtggtg gattcgagg gccgattcg ccgattcag gtggaggca gtgcagtcg gattcggag tttgaagtg aaaaatcgc aaaggcgac aacaaagat aatggccaa agctgggaa tggggcggc ttccccagc gtctcgccg acaaactcc ccaatcagc gaacaagcc accaaaaac gaccagttc gccatcagc aacgtcttc tccttcatg aaaaaatct cacggccag acggaagga atctactta tcggacctc aactcggtc gatccggaa gtggccgcc ctttatttc tcgacaaac aaaaataaa actacagat gccgacatg gattgtgaa tcaggaaat ggtccgtca ctggcccaa agcccgaac agtgctgaa gggtgcaag tcgatcgat tccgacttc gacgaacac atcaaaggg ggtcgcttg tactctaac gaagtctcg ttgtcgctg tgcggctgg gacccggaa ccggatgcc acgcgtttc gacgagcac atgatcacc ttcagtgac ttctgcaac aaccccatg ctgctggaa gacccgaat ttggtggtg aacatcaga gggaaatac tacaactgg aaagtagca gcccctatt atttcaagc atcatggtt ttcaacaga cctctcctg cagtcatca attgatcaa ctgtgcaac atacatatg ccctctaat caggagagc aaggacaat aaacaagaa gctaagacg tcgtggtgg cattggcgc agatctaag acttcaaga gaagcgacc ccgacggtt gatacgaga aaaacggta gtcgaagtt aaggacaca gctatagat atcggagat gagcacatc ctccaaacc gatatttca gtagcccag aaaattccc aaaactgaa gagactgaa gagaaggac aaggaatgt gccatctcg atctcgccc caagtgtgc caactttcg gaaaagtgc aggaagacc ttgcgcctc agctccaag caaattgcg agtttgaat ttgcgcgac ggcatgaac gaaatcgtg ttcagtgta acgacagcc tatcagggg acaacccgg tgcacctgc cacttgtac aagtggaaa tgggacgat aagatcgtc atttccgat atcgacggg accattacg aagtcggac gtgttgggc cacatcctg cccattgtc gggaaagac tgggcccaa tcgggcgtc gcccaattg tttaacaaa attaagggc aacggttac aaactattg tacctcagc gctcgagcg ataggccag gccagaatc acccgagag taccttagg agtatcaaa caggggaac ttgaccatg cccgatggt ccgattttg ctcaatccc acttcgttg atcacggct ttccaccga gaagtgatt gagaagaag cctgaacag ttcaagatt tcctgcatg agcgacatt aaggcgctg tttccgtct gaatcaaat ccgttctat gccggatac gggaatagg attaatgat gtgtgggcg taccgggct gtgggaatc cctatagtt agaattttt acaattaat cccaaaggg gagctcaag cacgagctc acacaaacc ttccagtcg acatattcg accatgacg tactacgta gaccagctg tttcctcca ttgatcgaa gctgccaat gactactcg cagttcgcc tattggcgc gatccactt ccggtcgtt gaagaactc ccggaagta gcgtaa
- >TC010029 RB
atgtattct atgaactat atcggacgc ttcttcagc aactttcgc gagttttac aacgaaatc aacacagcg acgctgacc ggagccatc gacgtcatc gtcgtggag caggaggat gggacgtac aagtgctca ccgttccac gtccgattt ggaaaactg ggagtcctc agaagtaga gaaaaagtg gtggacatc gaaatcaac ggcgaaccc gccgacatc cacatgaaa ctgggagag agcggggag gctttcttc gtcgaggag ctcgaagat gatgagaac gaaatcccg gaccatttg gccacgagc ccgatcccc gtcaacgaa ttcgagaac atcttcacg tcgcaaggc cggaggcgg agtttcggg gaacccccg actctcgaa aacgaaatc aacgattac acaaaacgg cgcaatacc gccgataac gagagcagc gtcaaacgc gagcgcgat tttctcaaa cgacagatc ggtttgggg aacatcggg atcggggag aacagcacg gaggacatg tccaggtcc atgcactcc accaccaag gaaagcgtg gaggacttg agcaaggac aacgacatt tcggacact atcttcaaa atggacagt ctggagatg gacaccagt cggtcggag agcgggcca aagcccgag gaagcgagt caggggaag gacgagcac agcagcgag agtcggagt agtaagaaa aagcggcgc aaaactaaa aagaaaaac gcgccgagg aagtcgtcc agtgttaat cagatatcg cccgaagtg gcggagaaa aatgactcg gcgacgacc gatcccagc tcgattgag agcaattcg tcggagccc gagttgaag gagttgcag aatgttgaa gggagtgct cagacgccg tcgaatagt cgcaagatc atcgatccg gattttcat ttcttcagc gatacagag ttgaccagt aatgtggtg gattcgagg gccgattcg ccgattcag gtggaggca gtgcagtcg gattcggag tttgaagtg aaaaatcgc aaaggcgac aacaaagat aatggccaa agctgggaa tggggcggc ttccccagc gtctcgccg acaaactcc ccaatcagc gaacaagcc accaaaaac gaccagttc gccatcagc aacgtcttc tccttcatg aaaaaatct cacggccag acggaagga atctactta tcggacctc aactcggtc gatccggaa gtggccgcc ctttatttc tcgacaaac aaaaataaa actacagat gccgacatg gattgtgaa tcaggaaat ggtccgtca ctggcccaa agcccgaac agtgctgaa gggtgcaag tcgatcgat tccgacttc gacgaacac atcaaaggg ggtcgcttg tactctaac gaagtctcg ttgtcgctg tgcggctgg gacccggaa ccggatgcc acgcgtttc gacgagcac atgatcacc ttcagtgac ttctgcaac aaccccatg ctgctggaa gacccgaat ttggtggtg aacatcaga gggaaatac tacaactgg aaagtagca gcccctatt atttcaagc atcatggtt ttcaacaga cctctcctg cagtcatca attgatcaa ctgtgcaac atacatatg ccctctaat caggagagc aaggacaat aaacaagaa gctaagacg tcgtggtgg cattggcgc agatctaag acttcaaga gaagcgacc ccgacggtt gatacgaga aaaacggta gtcgaagtt aaggacaca gctatagat atcggagat gagcacatc ctccaaacc gatatttca gtagcccag aaaattccc aaaactgaa gagactgaa gagaaggac aaggaatgt gccatctcg atctcgccc caagtgtgc caactttcg gaaaagtgc aggaagacc ttgcgcctc agctccaag caaattgcg agtttgaat ttgcgcgac ggcatgaac gaaatcgtg ttcagtgta acgacagcc tatcagggg acaacccgg tgcacctgc cacttgtac aagtggaaa tgggacgat aagatcgtc atttccgat atcgacggg accattacg aagtcggac gtgttgggc cacatcctg cccattgtc gggaaagac tgggcccaa tcgggcgtc gcccaattg tttaacaaa attaagggc aacggttac aaactattg tacctcagc gctcgagcg ataggccag gccagaatc acccgagag taccttagg agtatcaaa caggggaac ttgaccatg cccgatggt ccgattttg ctcaatccc acttcgttg atcacggct ttccaccga gaagtgatt gagaagaag cctgaacag ttcaagatt tcctgcatg agcgacatt aaggcgctg tttccgtct gaatcaaat ccgttctat gccggatac gggaatagg attaatgat gtgtgggcg taccgggct gtgggaatc cctatagtt agaattttt acaattaat cccaaaggg gagctcaag cacgagctc acacaaacc ttccagtcg acttacacg ggtcagtca ctggtcgtc aatgacgtg tttccccca cttttgcat aaactcaga gacaatgac actagtagt gacgaaata gagtcagat atgagcgat agcgattaa
- >TC010029 RA
tcccacttg tcaataatt tatctgtca gttgctaga cgtagtcac aggtgaatg tagctgacc gttttgtga atttatggt gcttgataa aagaaataa atcgtcaag attcaatta gattagatt gctagcgca ggacgcgcg cgccaggat aacaacaga cactataaa aatacgcgt gttttgttt ttccactca ttatcgagt tggaatctg tgtgaagtt caagtgctt gtgattaaa taaccaaaa tgtattcta tgaactata tcggacgct tcttcagca actttcgcg agttttaca acgaaatca acacagcga cgctgaccg gagccatcg acgtcatcg tcgtggagc aggaggatg ggacgtaca agtgctcac cgttccacg tccgatttg gaaaactgg gagtcctca gaagtagag aaaaagtgg tggacatcg aaatcaacg gcgaacccg ccgacatcc acatgaaac tgggagaga gcggggagg ctttcttcg tcgaggagc tcgaagatg atgagaacg aaatcccgg accatttgg ccacgagcc cgatccccg tcaacgaat tcgagaaca tcttcacgt cgcaaggcc ggaggcgga gtttcgggg aacccccga ctctcgaaa acgaaatca acgattaca caaaacggc gcaataccg ccgataacg agagcagcg tcaaacgcg agcgcgatt ttctcaaac gacagatcg gtttgggga acatcggga tcggggaga acagcacgg aggacatgt ccaggtcca tgcactcca ccaccaagg aaagcgtgg aggacttga gcaaggaca acgacattt cggacacta tcttcaaaa tggacagtc tggagatgg acaccagtc ggtcggaga gcgggccaa agcccgagg aagcgagtc aggggaagg acgagcaca gcagcgaga gtcggagta gtaagaaaa agcggcgca aaactaaaa agaaaaacg cgccgagga agtcgtcca gtgttaatc agatatcgc ccgaagtgg cggagaaaa atgactcgg cgacgaccg atcccagct cgattgaga gcaattcgt cggagcccg agttgaagg agttgcaga atgttgaag ggagtgctc agacgccgt cgaatagtc gcaagatca tcgatccgg attttcatt tcttcagcg atacagagt tgaccagta atgtggtgg attcgaggg ccgattcgc cgattcagg tggaggcag tgcagtcgg attcggagt ttgaagtga aaaatcgca aaggcgaca acaaagata atggccaaa gctgggaat ggggcggct tccccagcg tctcgccga caaactccc caatcagcg aacaagcca ccaaaaacg accagttcg ccatcagca acgtcttct ccttcatga aaaaatctc acggccaga cggaaggaa tctacttat cggacctca actcggtcg atccggaag tggccgccc tttatttct cgacaaaca aaaataaaa ctacagatg ccgacatgg attgtgaat caggaaatg gtccgtcac tggcccaaa gcccgaaca gtgctgaag ggtgcaagt cgatcgatt ccgacttcg acgaacaca tcaaagggg gtcgcttgt actctaacg aagtctcgt tgtcgctgt gcggctggg acccggaac cggatgcca cgcgtttcg acgagcaca tgatcacct tcagtgact tctgcaaca accccatgc tgctggaag acccgaatt tggtggtga acatcagag ggaaatact acaactgga aagtagcag cccctatta tttcaagca tcatggttt tcaacagac ctctcctgc agtcatcaa ttgatcaac tgtgcaaca tacatatgc cctctaatc aggagagca aggacaata aacaagaag ctaagacgt cgtggtggc attggcgca gatctaaga cttcaagag aagcgaccc cgacggttg atacgagaa aaacggtag tcgaagtta aggacacag ctatagata tcggagatg agcacatcc tccaaaccg atatttcag tagcccaga aaattccca aaactgaag agactgaag agaaggaca aggaatgtg ccatctcga tctcgcccc aagtgtgcc aactttcgg aaaagtgca ggaagacct tgcgcctca gctccaagc aaattgcga gtttgaatt tgcgcgacg gcatgaacg aaatcgtgt tcagtgtaa cgacagcct atcagggga caacccggt gcacctgcc acttgtaca agtggaaat gggacgata agatcgtca tttccgata tcgacggga ccattacga agtcggacg tgttgggcc acatcctgc ccattgtcg ggaaagact gggcccaat cgggcgtcg cccaattgt ttaacaaaa ttaagggca acggttaca aactattgt acctcagcg ctcgagcga taggccagg ccagaatca cccgagagt accttagga gtatcaaac aggggaact tgaccatgc ccgatggtc cgattttgc tcaatccca cttcgttga tcacggctt tccaccgag aagtgattg agaagaagc ctgaacagt tcaagattt cctgcatga gcgacatta aggcgctgt ttccgtctg aatcaaatc cgttctatg ccggatacg ggaatagga ttaatgatg tgtgggcgt accgggctg tgggaatcc ctatagtta gaattttta caattaatc ccaaagggg agctcaagc acgagctca cacaaacct tccagtcga catattcga ccatgacgt actacgtag accagctgt ttcctccat tgatcgaag ctgccaatg actactcgc agttcgcct attggcgcg atccacttc cggtcgttg aagaactcc cggaagtag cgtaaaatt gaatttgtc tgcatttat cgattcagt atttgttgt cgtgcgctt tcgatgtct ccattaccg gacacagct tttagaaca aatcaagta gctgtcact gttataaat tacaataat aacgataat gtgatatac tgattacca acttattgt aaggctagg cttaaatac tctacctgt taaacaaat tgtgcgttg ctttgttca attttagta ctaaaaagt gattcacta ctttttata ttgtgatat tttatttcg taagggata cactatttt ttttgtttt ttttttaag ttattgcaa taaagttcc aactgtata
- >TC010029 RB
tcccacttg tcaataatt tatctgtca gttgctaga cgtagtcac aggtgaatg tagctgacc gttttgtga atttatggt gcttgataa aagaaataa atcgtcaag attcaatta gattagatt gctagcgca ggacgcgcg cgccaggat aacaacaga cactataaa aatacgcgt gttttgttt ttccactca ttatcgagt tggaatctg tgtgaagtt caagtgctt gtgattaaa taaccaaaa tgtattcta tgaactata tcggacgct tcttcagca actttcgcg agttttaca acgaaatca acacagcga cgctgaccg gagccatcg acgtcatcg tcgtggagc aggaggatg ggacgtaca agtgctcac cgttccacg tccgatttg gaaaactgg gagtcctca gaagtagag aaaaagtgg tggacatcg aaatcaacg gcgaacccg ccgacatcc acatgaaac tgggagaga gcggggagg ctttcttcg tcgaggagc tcgaagatg atgagaacg aaatcccgg accatttgg ccacgagcc cgatccccg tcaacgaat tcgagaaca tcttcacgt cgcaaggcc ggaggcgga gtttcgggg aacccccga ctctcgaaa acgaaatca acgattaca caaaacggc gcaataccg ccgataacg agagcagcg tcaaacgcg agcgcgatt ttctcaaac gacagatcg gtttgggga acatcggga tcggggaga acagcacgg aggacatgt ccaggtcca tgcactcca ccaccaagg aaagcgtgg aggacttga gcaaggaca acgacattt cggacacta tcttcaaaa tggacagtc tggagatgg acaccagtc ggtcggaga gcgggccaa agcccgagg aagcgagtc aggggaagg acgagcaca gcagcgaga gtcggagta gtaagaaaa agcggcgca aaactaaaa agaaaaacg cgccgagga agtcgtcca gtgttaatc agatatcgc ccgaagtgg cggagaaaa atgactcgg cgacgaccg atcccagct cgattgaga gcaattcgt cggagcccg agttgaagg agttgcaga atgttgaag ggagtgctc agacgccgt cgaatagtc gcaagatca tcgatccgg attttcatt tcttcagcg atacagagt tgaccagta atgtggtgg attcgaggg ccgattcgc cgattcagg tggaggcag tgcagtcgg attcggagt ttgaagtga aaaatcgca aaggcgaca acaaagata atggccaaa gctgggaat ggggcggct tccccagcg tctcgccga caaactccc caatcagcg aacaagcca ccaaaaacg accagttcg ccatcagca acgtcttct ccttcatga aaaaatctc acggccaga cggaaggaa tctacttat cggacctca actcggtcg atccggaag tggccgccc tttatttct cgacaaaca aaaataaaa ctacagatg ccgacatgg attgtgaat caggaaatg gtccgtcac tggcccaaa gcccgaaca gtgctgaag ggtgcaagt cgatcgatt ccgacttcg acgaacaca tcaaagggg gtcgcttgt actctaacg aagtctcgt tgtcgctgt gcggctggg acccggaac cggatgcca cgcgtttcg acgagcaca tgatcacct tcagtgact tctgcaaca accccatgc tgctggaag acccgaatt tggtggtga acatcagag ggaaatact acaactgga aagtagcag cccctatta tttcaagca tcatggttt tcaacagac ctctcctgc agtcatcaa ttgatcaac tgtgcaaca tacatatgc cctctaatc aggagagca aggacaata aacaagaag ctaagacgt cgtggtggc attggcgca gatctaaga cttcaagag aagcgaccc cgacggttg atacgagaa aaacggtag tcgaagtta aggacacag ctatagata tcggagatg agcacatcc tccaaaccg atatttcag tagcccaga aaattccca aaactgaag agactgaag agaaggaca aggaatgtg ccatctcga tctcgcccc aagtgtgcc aactttcgg aaaagtgca ggaagacct tgcgcctca gctccaagc aaattgcga gtttgaatt tgcgcgacg gcatgaacg aaatcgtgt tcagtgtaa cgacagcct atcagggga caacccggt gcacctgcc acttgtaca agtggaaat gggacgata agatcgtca tttccgata tcgacggga ccattacga agtcggacg tgttgggcc acatcctgc ccattgtcg ggaaagact gggcccaat cgggcgtcg cccaattgt ttaacaaaa ttaagggca acggttaca aactattgt acctcagcg ctcgagcga taggccagg ccagaatca cccgagagt accttagga gtatcaaac aggggaact tgaccatgc ccgatggtc cgattttgc tcaatccca cttcgttga tcacggctt tccaccgag aagtgattg agaagaagc ctgaacagt tcaagattt cctgcatga gcgacatta aggcgctgt ttccgtctg aatcaaatc cgttctatg ccggatacg ggaatagga ttaatgatg tgtgggcgt accgggctg tgggaatcc ctatagtta gaattttta caattaatc ccaaagggg agctcaagc acgagctca cacaaacct tccagtcga cttacacgg gtcagtcac tggtcgtca atgacgtgt ttcccccac ttttgcata aactcagag acaatgaca ctagtagtg acgaaatag agtcagata tgagcgata gcgattaat ttgtatttt atcagtaca aacactctc atctagttc atgcaacta acggtggta gcgcttatc attcatcct catgatcat caggggctt atcgcgaac gcatccaat gataaaata caaatgagt tcgcgttta atttattta tttattgtt taaatgtta ttgtcttaa actccggcc ctctccgtg gtttattat aacggtaag cttaagtca gtat
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