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Gene information
General information
TC009984 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10706516..10708311
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009984
    iBeetle screen iB_01639

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009984 RA
      MADNEDLGL GPEDHLESL DETNGMHDD 
ALLNDTDLD GTGGDDPEL EAIKARVRE 
MEEEAEKLK QLQSEVDKQ MNMGSPPGL 
TSPLNMSLE EKMEVDNRS IYVGNVDYG 
ATAEELEQH FHGCGSINR VTILCNKYD 
GHPKGFAYI EFGDRDSVQ TAMAMDESL 
FRGRPIKVM PKRTNRPGL STTNRPPRG 
RGAFRGARG VGRGFYAGY RPMRRPRSY 
RRGGYFSPY
    CDS sequence
    • >TC009984 RA
      atggctgat aatgaagat ttgggatta ggtccagag 
gatcacttg gaatcttta gacgaaaca aatgggatg 
cacgacgac gctcttttg aacgacacc gatttggac 
ggcaccggg ggcgacgac cctgagctc gaagccatc 
aaagcccgg gtgcgtgag atggaagag gaagccgaa 
aagctgaaa cagctccag agcgaggtc gataaacaa 
atgaacatg ggcagtcct cccggacta accagtcct 
ttgaatatg tcgcttgag gagaagatg gaggttgat 
aatcgcagt atttacgtc ggcaatgtc gactacggc 
gcaacagcc gaagaactg gaacagcac ttccatggt 
tgtggatca ataaaccgg gtcactatt ctttgtaat 
aaatacgat ggacacccc aaaggcttc gcctacatt 
gagtttggg gacagggac tccgtgcag acggcgatg 
gcgatggac gagtcgttg tttagagga agaccaatc 
aaggtcatg cctaaaagg accaataga ccgggcctt 
tccaccaca aaccggccg ccaaggggc agaggggcg 
ttccggggg gcacgagga gtcgggcgg ggattttac 
gctggctat aggcccatg cgtagacca aggagttac 
agacgaggt ggatatttt tcaccatac tga
    mRNA sequence
    • >TC009984 RA
      ggcaacgca agtcaccta cgccatttt tcttcacaa 
gccgactct tccacaatt tgccggtaa taaaaaagt 
ggcttggat ggtgtgttt tagtgctct tttgacaaa 
aaattatct ccagtacgg cagcgttta catgtaaca 
gattgaaca aactatggc tgataatga agatttggg 
attaggtcc agaggatca cttggaatc tttagacga 
aacaaatgg gatgcacga cgacgctct tttgaacga 
caccgattt ggacggcac cgggggcga cgaccctga 
gctcgaagc catcaaagc ccgggtgcg tgagatgga 
agaggaagc cgaaaagct gaaacagct ccagagcga 
ggtcgataa acaaatgaa catgggcag tcctcccgg 
actaaccag tcctttgaa tatgtcgct tgaggagaa 
gatggaggt tgataatcg cagtattta cgtcggcaa 
tgtcgacta cggcgcaac agccgaaga actggaaca 
gcacttcca tggttgtgg atcaataaa ccgggtcac 
tattctttg taataaata cgatggaca ccccaaagg 
cttcgccta cattgagtt tggggacag ggactccgt 
gcagacggc gatggcgat ggacgagtc gttgtttag 
aggaagacc aatcaaggt catgcctaa aaggaccaa 
tagaccggg cctttccac cacaaaccg gccgccaag 
gggcagagg ggcgttccg gggggcacg aggagtcgg 
gcggggatt ttacgctgg ctataggcc catgcgtag 
accaaggag ttacagacg aggtggata tttttcacc 
atactgacg ggcctggta aatataagg aacatcatt 
attacattt ttgtttaag taacacctc tcataattc 
tatcacact gatattttg gactaagaa aaaaaagtt 
acaaaagaa aaaagaaaa aaagtgaag ttaaaaact 
gtaaattaa aaacatttc ccctcgtaa aatttaaat 
gttaggcta acatgtatg agttgagcg agtgttgcg 
tgcatgtgt ggaaattaa tttttcttc atagtcaga 
agaaaacaa agaaagtca taagataat tatgagttt 
ctttcgttc attgggtga gaaattggt agttgtaac 
ggttatgag aatagtttt gttacggtc tgaacagtt 
tcgttttta ttttataat ttttaatgt tgcaacatt 
acaggtacc ttcagcctg tttttttca ggagttcca 
tttttttaa aagttagat tagaaaata acaaaaatg 
taaaaaaaa ttcatgtac atgtaaaga tgaataata 
aattggtga aatataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY