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Gene information
General information
TC009968 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 4584337..4585231
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009968
    iBeetle screen iB_01636
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009968 RA
      MSKNRELLS KIGLRQDGR RADELRRIR CKLGVFTEP 
      DGSAYLEQG LTKVLAAVY GPHQVRGSR SKAQHDSAV 
      VNCQFSMAV FSTGERKKR PRGDRKSTE ISIHLRQAL 
      TAAIKVELY PWTQIDVYV EVLHADGGI YPACVNAAT 
      LALIDAGIP LKEYVCACT ASLANNDVP LLDVSHQEE 
      IIGGPTLTV AALPMSGKI VLMEMSQRF HVDHLQKVL 
      DKALQGCKD IKSILDEAV KSHIQDVGS STGWASTT
    CDS sequence
    • >TC009968 RA
      atgagcaaa aatcgcgaa ttattatca aaaatcggc 
      ttaagacag gacggaaga cgcgccgac gagttacgg 
      aggatccgc tgcaagctg ggcgtgttc actgaaccc 
      gacggtagc gcctatttg gagcagggc ttgactaaa 
      gttctggcg gctgtgtat gggccccac caggtccgc 
      ggcagtcgc agcaaagcg caacatgac tcggctgtg 
      gtcaactgc cagtttagc atggctgtg ttttcaaca 
      ggggagagg aagaagcgg ccccggggg gaccgcaag 
      tcgactgaa atctccatt cacttgagg caggcttta 
      actgcggcg atcaaagtc gagttgtat ccctggacc 
      cagattgat gtttatgtt gaggtgctg catgcagat 
      gggggcatt tatccggct tgtgtgaac gccgccact 
      ttggcactg attgatgcg gggattccg ttaaaggaa 
      tatgtctgt gcgtgtact gcaagtttg gcaaataat 
      gatgtgccg ctacttgat gtttcgcac caggaagag 
      ataataggg gggcccacg ttgacagtg gcagcgtta 
      ccaatgtcg gggaagatt gtcttgatg gaaatgtca 
      cagaggttt catgtggac cacttacag aaggtttta 
      gacaaggct ttacaaggg tgtaaagac attaaaagt 
      attttggat gaggcagtc aagagtcac attcaggat 
      gttggtagt tcgacaggt tgggcaagc acaacgtga
    mRNA sequence
    • >TC009968 RA
      caggatcgc caacgttac atttaaata attttttaa 
      cctcacttt taaacatca gaatgagca aaaatcgcg 
      aattattat caaaaatcg gcttaagac aggacggaa 
      gacgcgccg acgagttac ggaggatcc gctgcaagc 
      tgggcgtgt tcactgaac ccgacggta gcgcctatt 
      tggagcagg gcttgacta aagttctgg cggctgtgt 
      atgggcccc accaggtcc gcggcagtc gcagcaaag 
      cgcaacatg actcggctg tggtcaact gccagttta 
      gcatggctg tgttttcaa caggggaga ggaagaagc 
      ggccccggg gggaccgca agtcgactg aaatctcca 
      ttcacttga ggcaggctt taactgcgg cgatcaaag 
      tcgagttgt atccctgga cccagattg atgtttatg 
      ttgaggtgc tgcatgcag atgggggca tttatccgg 
      cttgtgtga acgccgcca ctttggcac tgattgatg 
      cggggattc cgttaaagg aatatgtct gtgcgtgta 
      ctgcaagtt tggcaaata atgatgtgc cgctacttg 
      atgtttcgc accaggaag agataatag gggggccca 
      cgttgacag tggcagcgt taccaatgt cggggaaga 
      ttgtcttga tggaaatgt cacagaggt ttcatgtgg 
      accacttac agaaggttt tagacaagg ctttacaag 
      ggtgtaaag acattaaaa gtattttgg atgaggcag 
      tcaagagtc acattcagg atgttggta gttcgacag 
      gttgggcaa gcacaacgt gaggagagc atgtataga 
      atatatttc tgacaatta t