Status
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Gene information
General information
TC033768
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009960 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11306364..11328738
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033768
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01635

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 4 (41 dpi)
    Days post injection

    Pupal morphology
    dorsal thoracic muscles mostlynot present - Number of animals showing the phenotype: 9

    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects -
    Comment:
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033768 RA
      MFVVLVFKS KVPLIPSLA WSFCRGLLS CPEAHEFRE 
VQCSAYNSI PYQGNLYEW SAHYNDSNP CALTCKGKP 
ISESSNEDP VLVVVKLAD KVHDGTRCR LGSLDMCID 
GECQRVGCD LRIGSTRQV DECGVCGGD GSSCSRPLY 
HWTLTYMSL CSVTCGGGY KMSRPLCQN RVTGEEVEE 
ELCNESQKP DSTIVECNT HNCPPKWHM GEWGPCSVS 
CGGGSRLRQ VYCVEEANS TKIRVNDQK CTGHKPWFQ 
EACNRMDCP AWIASSWSG CSVSCGEGT QVRVVECRD 
SADKPSTLC PQKLKPIDS KPCSTGIQC PHHIDASEE 
FLPGLYHTQ PLVQPYPPP PPAHAERLV GEQVVPSES 
TFVPDEWGP CSVTCGEGV RKRQVHCKI FLEFSRTIA 
KLPDKQCTG PKPIETEKC YMEPCSAER IGVDIKDDP 
YRSDIKVAG GFPGKSYSW KEQGYTHCS ATCLGGVQE 
LIVNCVRDD TQKVTSPYL CPIELKPEI LMRTCNDHP 
CPPRWNYSD FSTCSQSCG IGIQTREVN CIHEVTQGG 
GNTVIVPNN MCPQPPPPD RQYCNVLDC PVRWKVSEW 
SRCSKPCGG GEKIRKVEC KQVMAQNHT VDRSPELCP 
SPKPPDRKP CNTKSCIIE SDKPHIDVS NSSFIQHDF 
KKKKIALKV GGSATVFIG TTVKIKCPV KRFNRTKIQ 
WVKDKNFLP KSKKYKTSK KGALRVQNL TLRDSGRYT 
CVAGRSSAS IQISVRPKP GEFLTSEEI QKQGKPDRS 
ELISDVYVN REDTSPIFS SDDHSHEQR PDVVRKKPT 
PKTRILAPT LPALNSNNL NAYLPQSTT LHPDNVHQL 
WPFQTFSNS RGHRMVAFP EESFQENSF APPTTEDEF 
GSVVVLGKG TPENLKFEW VISDWSKCS QTCGGNGFQ 
MRAIRCIVR LHNTTHGVD HNFCEDAGM PIPATERKC 
GLNDCPRWV TSEWTPCDK SKCFDWHTA MQRRLVKCQ 
IEGNVTVAE SKCNEEDKP THRQECANE RCVGKWKLG 
AWSECSADC EMQGFKYRI LQCVWYGTK KPAGTACKD 
LPRPPVMKT CKGPPCSKS ESQCKDHSM FCSNVKTMN 
MCKITRYQQ QCCKTCKAG
    CDS sequence
    • >TC033768 RA
      atgtttgtg gtgctcgtt ttcaagagc aaggttccg 
ttgattccg tcactggcg tggtctttt tgccgtggg 
cttctttcg tgcccagag gctcacgaa ttccgcgag 
gtccagtgc tcagcatac aacagcatt ccctaccag 
ggaaatctt tacgagtgg tcagcacat tacaatgac 
tcgaacccg tgcgctctt acctgcaaa ggcaagccg 
attagcgag tcaagtaat gaggatcct gtgcttgtt 
gtggtaaag cttgcagat aaagttcat gacggaact 
agatgcaga ctgggaagt ctggacatg tgtatcgat 
ggagaatgt cagagagtg gggtgtgat ctgaggatt 
ggctcgacg agacaagtg gatgaatgt ggagtttgt 
ggcggagat ggatcgtca tgttcgagg cctttgtac 
cactggact ctgacgtat atgtcgttg tgctcggtg 
acatgtggc gggggctac aaaatgtca cgtccctta 
tgtcagaac cgggtgact ggagaggaa gttgaggaa 
gaactttgt aacgagtca caaaagccc gattcgacg 
atagtcgag tgtaacacc cataactgc cctcccaag 
tggcacatg ggtgagtgg ggcccgtgc tcagtgagc 
tgtggtgga gggtcaaga cttcggcaa gtttattgc 
gtggaagaa gccaacagt actaaaatt agggttaat 
gatcagaag tgcaccgga cacaagccg tggtttcag 
gaagcctgc aatcgaatg gattgtccg gcctggatt 
gcatcgtcc tggtcagga tgctcggtg tcttgcggg 
gaaggcacg caagtgagg gtggttgaa tgccgagat 
tccgcagac aagcccagc acattgtgc cctcagaaa 
cttaagcct attgacagc aagccctgt tcgacgggc 
attcagtgt ccgcatcac atagatgcc agcgaggag 
tttttgccc ggcctctat cacactcaa cctttggtc 
cagccgtac cctccgccg cccccagct catgccgaa 
cgactcgtc ggggagcag gttgtgccc tcggaaagc 
acatttgtt cccgacgag tggggccct tgcagcgtg 
acttgcggc gaaggcgtc cgcaaacgt caagtccac 
tgcaagatc ttcctcgaa ttttcaaga actatcgcc 
aaactccca gacaaacag tgtaccggt cctaaacca 
atagagaca gaaaaatgc tacatggaa ccatgctca 
gccgaacgc atcggggtc gatattaaa gacgacccc 
tatcgatcg gacatcaaa gtcgccggg ggcttcccc 
ggaaagtcc tactcgtgg aaggagcaa ggctacacc 
cactgcagc gccacgtgt ctcggaggc gtccaagag 
ctgatagtc aactgtgtc cgcgacgac acgcagaaa 
gtcacctcc ccgtacttg tgccccatc gagctcaag 
cccgaaata ctcatgagg acgtgcaac gaccacccg 
tgcccccct cggtggaac tactccgat ttttcgact 
tgttcgcaa agctgcggc atcgggatt cagaccagg 
gaggtcaac tgcattcac gaggtgacc caaggcggg 
ggcaacact gtaatagtc ccgaataac atgtgcccg 
caaccgccg cccccagac cggcagtat tgcaacgtc 
ctggactgc ccagtcagg tggaaagtg tcggaatgg 
tccaggtgt agcaagcca tgtggcggt ggtgaaaaa 
atcaggaaa gtggagtgt aagcaagtc atggcccaa 
aaccacaca gtggacaga tcacccgag ctgtgcccc 
agtcccaag ccacccgac cggaagcct tgtaacacc 
aaaagttgc atcattgaa agtgacaag ccccacatt 
gacgtttca aacagttcg ttcattcag cacgatttc 
aagaagaag aaaattgcg ttgaaagtc ggaggctca 
gctactgtt tttattgga accacggtt aaaatcaaa 
tgcccggtc aagagattc aaccgcacg aaaatccag 
tgggtcaaa gacaagaac ttcctgccc aagtccaag 
aaatacaag acgtccaag aagggggcc ttgcgggtg 
caaaatctg acgctccgc gacagcggc cgctacact 
tgcgtggca ggccgttcc tccgccagt atccaaatt 
tccgttcgc cccaaaccc ggcgaattc ctcacatcc 
gaggagatc cagaagcag ggcaagccc gaccgcagc 
gagctaata tccgacgtg tacgtcaac agagaggac 
acgagtcca attttcagc agcgacgat cactcgcac 
gagcaaagg cccgacgtc gtgagaaag aagccgacg 
cccaagaca cggatattg gcgcccacg ctgcccgcc 
ctcaactcg aacaacctg aacgcttac ttgccgcag 
agcacgacg ctgcacccg gacaacgtg catcaattg 
tggcccttc cagacattc agcaactcg cgaggccat 
cgcatggtg gccttcccg gaggagtcc ttccaggag 
aactcgttc gcgccgcca acgaccgaa gacgaattc 
ggttcggtc gtagttctg ggtaaagga actccggaa 
aacttaaaa ttcgaatgg gtcatttcc gattggtcg 
aaatgctcg caaacctgc ggcggaaat ggctttcag 
atgagggca attcgttgc attgttcga ctacataat 
acgacacac ggcgtcgat cataacttt tgcgaggac 
gctggcatg ccaattcca gcgacggaa cgaaaatgc 
ggactgaac gactgtccc agatgggtg acttccgaa 
tggacgccc tgcgacaag tccaagtgc ttcgattgg 
cacacagca atgcagagg agattggtc aagtgccag 
attgaaggg aatgtcacg gtggcggag tccaagtgc 
aacgaagag gataagccg acgcacagg caggagtgc 
gccaacgag aggtgtgtg ggcaagtgg aagctcgga 
gcttggtca gaatgctct gcggattgc gaaatgcaa 
ggattcaag tacagaatc ctgcaatgt gtttggtat 
ggcacgaaa aaacctgca gggactgcc tgtaaagac 
cttccccgt cctccagtc atgaagact tgtaaagga 
cctccgtgc tccaaatca gagtcgcaa tgcaaagac 
cactctatg ttttgttcg aacgtgaaa acgatgaac 
atgtgcaaa ataacgagg taccaacag caatgctgc 
aagacgtgc aaggccggt tag
    mRNA sequence
    • >TC033768 RA
      tgaaagttc cgcacatgt ttgtggtgc tcgttttca 
agagcaagg ttccgttga ttccgtcac tggcgtggt 
ctttttgcc gtgggcttc tttcgtgcc cagaggctc 
acgaattcc gcgaggtcc agtgctcag catacaaca 
gcattccct accagggaa atctttacg agtggtcag 
cacattaca atgactcga acccgtgcg ctcttacct 
gcaaaggca agccgatta gcgagtcaa gtaatgagg 
atcctgtgc ttgttgtgg taaagcttg cagataaag 
ttcatgacg gaactagat gcagactgg gaagtctgg 
acatgtgta tcgatggag aatgtcaga gagtggggt 
gtgatctga ggattggct cgacgagac aagtggatg 
aatgtggag tttgtggcg gagatggat cgtcatgtt 
cgaggcctt tgtaccact ggactctga cgtatatgt 
cgttgtgct cggtgacat gtggcgggg gctacaaaa 
tgtcacgtc ccttatgtc agaaccggg tgactggag 
aggaagttg aggaagaac tttgtaacg agtcacaaa 
agcccgatt cgacgatag tcgagtgta acacccata 
actgccctc ccaagtggc acatgggtg agtggggcc 
cgtgctcag tgagctgtg gtggagggt caagacttc 
ggcaagttt attgcgtgg aagaagcca acagtacta 
aaattaggg ttaatgatc agaagtgca ccggacaca 
agccgtggt ttcaggaag cctgcaatc gaatggatt 
gtccggcct ggattgcat cgtcctggt caggatgct 
cggtgtctt gcggggaag gcacgcaag tgagggtgg 
ttgaatgcc gagattccg cagacaagc ccagcacat 
tgtgccctc agaaactta agcctattg acagcaagc 
cctgttcga cgggcattc agtgtccgc atcacatag 
atgccagcg aggagtttt tgcccggcc tctatcaca 
ctcaacctt tggtccagc cgtaccctc cgccgcccc 
cagctcatg ccgaacgac tcgtcgggg agcaggttg 
tgccctcgg aaagcacat ttgttcccg acgagtggg 
gcccttgca gcgtgactt gcggcgaag gcgtccgca 
aacgtcaag tccactgca agatcttcc tcgaatttt 
caagaacta tcgccaaac tcccagaca aacagtgta 
ccggtccta aaccaatag agacagaaa aatgctaca 
tggaaccat gctcagccg aacgcatcg gggtcgata 
ttaaagacg acccctatc gatcggaca tcaaagtcg 
ccgggggct tccccggaa agtcctact cgtggaagg 
agcaaggct acacccact gcagcgcca cgtgtctcg 
gaggcgtcc aagagctga tagtcaact gtgtccgcg 
acgacacgc agaaagtca cctccccgt acttgtgcc 
ccatcgagc tcaagcccg aaatactca tgaggacgt 
gcaacgacc acccgtgcc cccctcggt ggaactact 
ccgattttt cgacttgtt cgcaaagct gcggcatcg 
ggattcaga ccagggagg tcaactgca ttcacgagg 
tgacccaag gcgggggca acactgtaa tagtcccga 
ataacatgt gcccgcaac cgccgcccc cagaccggc 
agtattgca acgtcctgg actgcccag tcaggtgga 
aagtgtcgg aatggtcca ggtgtagca agccatgtg 
gcggtggtg aaaaaatca ggaaagtgg agtgtaagc 
aagtcatgg cccaaaacc acacagtgg acagatcac 
ccgagctgt gccccagtc ccaagccac ccgaccgga 
agccttgta acaccaaaa gttgcatca ttgaaagtg 
acaagcccc acattgacg tttcaaaca gttcgttca 
ttcagcacg atttcaaga agaagaaaa ttgcgttga 
aagtcggag gctcagcta ctgttttta ttggaacca 
cggttaaaa tcaaatgcc cggtcaaga gattcaacc 
gcacgaaaa tccagtggg tcaaagaca agaacttcc 
tgcccaagt ccaagaaat acaagacgt ccaagaagg 
gggccttgc gggtgcaaa atctgacgc tccgcgaca 
gcggccgct acacttgcg tggcaggcc gttcctccg 
ccagtatcc aaatttccg ttcgcccca aacccggcg 
aattcctca catccgagg agatccaga agcagggca 
agcccgacc gcagcgagc taatatccg acgtgtacg 
tcaacagag aggacacga gtccaattt tcagcagcg 
acgatcact cgcacgagc aaaggcccg acgtcgtga 
gaaagaagc cgacgccca agacacgga tattggcgc 
ccacgctgc ccgccctca actcgaaca acctgaacg 
cttacttgc cgcagagca cgacgctgc acccggaca 
acgtgcatc aattgtggc ccttccaga cattcagca 
actcgcgag gccatcgca tggtggcct tcccggagg 
agtccttcc aggagaact cgttcgcgc cgccaacga 
ccgaagacg aattcggtt cggtcgtag ttctgggta 
aaggaactc cggaaaact taaaattcg aatgggtca 
tttccgatt ggtcgaaat gctcgcaaa cctgcggcg 
gaaatggct ttcagatga gggcaattc gttgcattg 
ttcgactac ataatacga cacacggcg tcgatcata 
acttttgcg aggacgctg gcatgccaa ttccagcga 
cggaacgaa aatgcggac tgaacgact gtcccagat 
gggtgactt ccgaatgga cgccctgcg acaagtcca 
agtgcttcg attggcaca cagcaatgc agaggagat 
tggtcaagt gccagattg aagggaatg tcacggtgg 
cggagtcca agtgcaacg aagaggata agccgacgc 
acaggcagg agtgcgcca acgagaggt gtgtgggca 
agtggaagc tcggagctt ggtcagaat gctctgcgg 
attgcgaaa tgcaaggat tcaagtaca gaatcctgc 
aatgtgttt ggtatggca cgaaaaaac ctgcaggga 
ctgcctgta aagaccttc cccgtcctc cagtcatga 
agacttgta aaggacctc cgtgctcca aatcagagt 
cgcaatgca aagaccact ctatgtttt gttcgaacg 
tgaaaacga tgaacatgt gcaaaataa cgaggtacc 
aacagcaat gctgcaaga cgtgcaagg ccggttaga 
gttcgaaga gtctccgaa aaggcttaa attaatatt 
ttgagacgg tggagttat gttgtgtgg gcaaagcca 
agtgtgaag tgtgttcaa aagtgactt atatcatct 
attaaatct cttggacaa gtgctgcac ttagcaata 
gtagaagat aggaaatac gtttgaatc tcagcttgt 
gctgatctg atgtatatt aatgtactc gtcgtgttc 
agtggcgaa tttttgccc tttgatgga tgtttttgt 
gagagggta aaaatcgcg agtgagaac gacacattc 
aagactgat gttttttgt tacaattta tttgttgga 
tactatata catcccatg acattccat tattaccgg 
tattaaata ttaggtccg tcgtaacat gcaacggtt 
tcaaaggta gttaagtat aaaattttg tttttcgtg 
taaccgacc agaaatttt tgggttatt gcatactta 
aacctcaac acgtacctg cttttatta ttctgtaat 
aatacatgt atttttgta tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY
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