General information | |||
TC033768
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 11306364..11328738 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
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TC033768 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01635 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 3 (22 dpi)
adult lethality - Number of animals showing the phenotype: 4 (41 dpi)
- >TC033768 RA
MFVVLVFKS KVPLIPSLA WSFCRGLLS CPEAHEFRE VQCSAYNSI PYQGNLYEW SAHYNDSNP CALTCKGKP ISESSNEDP VLVVVKLAD KVHDGTRCR LGSLDMCID GECQRVGCD LRIGSTRQV DECGVCGGD GSSCSRPLY HWTLTYMSL CSVTCGGGY KMSRPLCQN RVTGEEVEE ELCNESQKP DSTIVECNT HNCPPKWHM GEWGPCSVS CGGGSRLRQ VYCVEEANS TKIRVNDQK CTGHKPWFQ EACNRMDCP AWIASSWSG CSVSCGEGT QVRVVECRD SADKPSTLC PQKLKPIDS KPCSTGIQC PHHIDASEE FLPGLYHTQ PLVQPYPPP PPAHAERLV GEQVVPSES TFVPDEWGP CSVTCGEGV RKRQVHCKI FLEFSRTIA KLPDKQCTG PKPIETEKC YMEPCSAER IGVDIKDDP YRSDIKVAG GFPGKSYSW KEQGYTHCS ATCLGGVQE LIVNCVRDD TQKVTSPYL CPIELKPEI LMRTCNDHP CPPRWNYSD FSTCSQSCG IGIQTREVN CIHEVTQGG GNTVIVPNN MCPQPPPPD RQYCNVLDC PVRWKVSEW SRCSKPCGG GEKIRKVEC KQVMAQNHT VDRSPELCP SPKPPDRKP CNTKSCIIE SDKPHIDVS NSSFIQHDF KKKKIALKV GGSATVFIG TTVKIKCPV KRFNRTKIQ WVKDKNFLP KSKKYKTSK KGALRVQNL TLRDSGRYT CVAGRSSAS IQISVRPKP GEFLTSEEI QKQGKPDRS ELISDVYVN REDTSPIFS SDDHSHEQR PDVVRKKPT PKTRILAPT LPALNSNNL NAYLPQSTT LHPDNVHQL WPFQTFSNS RGHRMVAFP EESFQENSF APPTTEDEF GSVVVLGKG TPENLKFEW VISDWSKCS QTCGGNGFQ MRAIRCIVR LHNTTHGVD HNFCEDAGM PIPATERKC GLNDCPRWV TSEWTPCDK SKCFDWHTA MQRRLVKCQ IEGNVTVAE SKCNEEDKP THRQECANE RCVGKWKLG AWSECSADC EMQGFKYRI LQCVWYGTK KPAGTACKD LPRPPVMKT CKGPPCSKS ESQCKDHSM FCSNVKTMN MCKITRYQQ QCCKTCKAG
- >TC033768 RA
atgtttgtg gtgctcgtt ttcaagagc aaggttccg ttgattccg tcactggcg tggtctttt tgccgtggg cttctttcg tgcccagag gctcacgaa ttccgcgag gtccagtgc tcagcatac aacagcatt ccctaccag ggaaatctt tacgagtgg tcagcacat tacaatgac tcgaacccg tgcgctctt acctgcaaa ggcaagccg attagcgag tcaagtaat gaggatcct gtgcttgtt gtggtaaag cttgcagat aaagttcat gacggaact agatgcaga ctgggaagt ctggacatg tgtatcgat ggagaatgt cagagagtg gggtgtgat ctgaggatt ggctcgacg agacaagtg gatgaatgt ggagtttgt ggcggagat ggatcgtca tgttcgagg cctttgtac cactggact ctgacgtat atgtcgttg tgctcggtg acatgtggc gggggctac aaaatgtca cgtccctta tgtcagaac cgggtgact ggagaggaa gttgaggaa gaactttgt aacgagtca caaaagccc gattcgacg atagtcgag tgtaacacc cataactgc cctcccaag tggcacatg ggtgagtgg ggcccgtgc tcagtgagc tgtggtgga gggtcaaga cttcggcaa gtttattgc gtggaagaa gccaacagt actaaaatt agggttaat gatcagaag tgcaccgga cacaagccg tggtttcag gaagcctgc aatcgaatg gattgtccg gcctggatt gcatcgtcc tggtcagga tgctcggtg tcttgcggg gaaggcacg caagtgagg gtggttgaa tgccgagat tccgcagac aagcccagc acattgtgc cctcagaaa cttaagcct attgacagc aagccctgt tcgacgggc attcagtgt ccgcatcac atagatgcc agcgaggag tttttgccc ggcctctat cacactcaa cctttggtc cagccgtac cctccgccg cccccagct catgccgaa cgactcgtc ggggagcag gttgtgccc tcggaaagc acatttgtt cccgacgag tggggccct tgcagcgtg acttgcggc gaaggcgtc cgcaaacgt caagtccac tgcaagatc ttcctcgaa ttttcaaga actatcgcc aaactccca gacaaacag tgtaccggt cctaaacca atagagaca gaaaaatgc tacatggaa ccatgctca gccgaacgc atcggggtc gatattaaa gacgacccc tatcgatcg gacatcaaa gtcgccggg ggcttcccc ggaaagtcc tactcgtgg aaggagcaa ggctacacc cactgcagc gccacgtgt ctcggaggc gtccaagag ctgatagtc aactgtgtc cgcgacgac acgcagaaa gtcacctcc ccgtacttg tgccccatc gagctcaag cccgaaata ctcatgagg acgtgcaac gaccacccg tgcccccct cggtggaac tactccgat ttttcgact tgttcgcaa agctgcggc atcgggatt cagaccagg gaggtcaac tgcattcac gaggtgacc caaggcggg ggcaacact gtaatagtc ccgaataac atgtgcccg caaccgccg cccccagac cggcagtat tgcaacgtc ctggactgc ccagtcagg tggaaagtg tcggaatgg tccaggtgt agcaagcca tgtggcggt ggtgaaaaa atcaggaaa gtggagtgt aagcaagtc atggcccaa aaccacaca gtggacaga tcacccgag ctgtgcccc agtcccaag ccacccgac cggaagcct tgtaacacc aaaagttgc atcattgaa agtgacaag ccccacatt gacgtttca aacagttcg ttcattcag cacgatttc aagaagaag aaaattgcg ttgaaagtc ggaggctca gctactgtt tttattgga accacggtt aaaatcaaa tgcccggtc aagagattc aaccgcacg aaaatccag tgggtcaaa gacaagaac ttcctgccc aagtccaag aaatacaag acgtccaag aagggggcc ttgcgggtg caaaatctg acgctccgc gacagcggc cgctacact tgcgtggca ggccgttcc tccgccagt atccaaatt tccgttcgc cccaaaccc ggcgaattc ctcacatcc gaggagatc cagaagcag ggcaagccc gaccgcagc gagctaata tccgacgtg tacgtcaac agagaggac acgagtcca attttcagc agcgacgat cactcgcac gagcaaagg cccgacgtc gtgagaaag aagccgacg cccaagaca cggatattg gcgcccacg ctgcccgcc ctcaactcg aacaacctg aacgcttac ttgccgcag agcacgacg ctgcacccg gacaacgtg catcaattg tggcccttc cagacattc agcaactcg cgaggccat cgcatggtg gccttcccg gaggagtcc ttccaggag aactcgttc gcgccgcca acgaccgaa gacgaattc ggttcggtc gtagttctg ggtaaagga actccggaa aacttaaaa ttcgaatgg gtcatttcc gattggtcg aaatgctcg caaacctgc ggcggaaat ggctttcag atgagggca attcgttgc attgttcga ctacataat acgacacac ggcgtcgat cataacttt tgcgaggac gctggcatg ccaattcca gcgacggaa cgaaaatgc ggactgaac gactgtccc agatgggtg acttccgaa tggacgccc tgcgacaag tccaagtgc ttcgattgg cacacagca atgcagagg agattggtc aagtgccag attgaaggg aatgtcacg gtggcggag tccaagtgc aacgaagag gataagccg acgcacagg caggagtgc gccaacgag aggtgtgtg ggcaagtgg aagctcgga gcttggtca gaatgctct gcggattgc gaaatgcaa ggattcaag tacagaatc ctgcaatgt gtttggtat ggcacgaaa aaacctgca gggactgcc tgtaaagac cttccccgt cctccagtc atgaagact tgtaaagga cctccgtgc tccaaatca gagtcgcaa tgcaaagac cactctatg ttttgttcg aacgtgaaa acgatgaac atgtgcaaa ataacgagg taccaacag caatgctgc aagacgtgc aaggccggt tag
- >TC033768 RA
tgaaagttc cgcacatgt ttgtggtgc tcgttttca agagcaagg ttccgttga ttccgtcac tggcgtggt ctttttgcc gtgggcttc tttcgtgcc cagaggctc acgaattcc gcgaggtcc agtgctcag catacaaca gcattccct accagggaa atctttacg agtggtcag cacattaca atgactcga acccgtgcg ctcttacct gcaaaggca agccgatta gcgagtcaa gtaatgagg atcctgtgc ttgttgtgg taaagcttg cagataaag ttcatgacg gaactagat gcagactgg gaagtctgg acatgtgta tcgatggag aatgtcaga gagtggggt gtgatctga ggattggct cgacgagac aagtggatg aatgtggag tttgtggcg gagatggat cgtcatgtt cgaggcctt tgtaccact ggactctga cgtatatgt cgttgtgct cggtgacat gtggcgggg gctacaaaa tgtcacgtc ccttatgtc agaaccggg tgactggag aggaagttg aggaagaac tttgtaacg agtcacaaa agcccgatt cgacgatag tcgagtgta acacccata actgccctc ccaagtggc acatgggtg agtggggcc cgtgctcag tgagctgtg gtggagggt caagacttc ggcaagttt attgcgtgg aagaagcca acagtacta aaattaggg ttaatgatc agaagtgca ccggacaca agccgtggt ttcaggaag cctgcaatc gaatggatt gtccggcct ggattgcat cgtcctggt caggatgct cggtgtctt gcggggaag gcacgcaag tgagggtgg ttgaatgcc gagattccg cagacaagc ccagcacat tgtgccctc agaaactta agcctattg acagcaagc cctgttcga cgggcattc agtgtccgc atcacatag atgccagcg aggagtttt tgcccggcc tctatcaca ctcaacctt tggtccagc cgtaccctc cgccgcccc cagctcatg ccgaacgac tcgtcgggg agcaggttg tgccctcgg aaagcacat ttgttcccg acgagtggg gcccttgca gcgtgactt gcggcgaag gcgtccgca aacgtcaag tccactgca agatcttcc tcgaatttt caagaacta tcgccaaac tcccagaca aacagtgta ccggtccta aaccaatag agacagaaa aatgctaca tggaaccat gctcagccg aacgcatcg gggtcgata ttaaagacg acccctatc gatcggaca tcaaagtcg ccgggggct tccccggaa agtcctact cgtggaagg agcaaggct acacccact gcagcgcca cgtgtctcg gaggcgtcc aagagctga tagtcaact gtgtccgcg acgacacgc agaaagtca cctccccgt acttgtgcc ccatcgagc tcaagcccg aaatactca tgaggacgt gcaacgacc acccgtgcc cccctcggt ggaactact ccgattttt cgacttgtt cgcaaagct gcggcatcg ggattcaga ccagggagg tcaactgca ttcacgagg tgacccaag gcgggggca acactgtaa tagtcccga ataacatgt gcccgcaac cgccgcccc cagaccggc agtattgca acgtcctgg actgcccag tcaggtgga aagtgtcgg aatggtcca ggtgtagca agccatgtg gcggtggtg aaaaaatca ggaaagtgg agtgtaagc aagtcatgg cccaaaacc acacagtgg acagatcac ccgagctgt gccccagtc ccaagccac ccgaccgga agccttgta acaccaaaa gttgcatca ttgaaagtg acaagcccc acattgacg tttcaaaca gttcgttca ttcagcacg atttcaaga agaagaaaa ttgcgttga aagtcggag gctcagcta ctgttttta ttggaacca cggttaaaa tcaaatgcc cggtcaaga gattcaacc gcacgaaaa tccagtggg tcaaagaca agaacttcc tgcccaagt ccaagaaat acaagacgt ccaagaagg gggccttgc gggtgcaaa atctgacgc tccgcgaca gcggccgct acacttgcg tggcaggcc gttcctccg ccagtatcc aaatttccg ttcgcccca aacccggcg aattcctca catccgagg agatccaga agcagggca agcccgacc gcagcgagc taatatccg acgtgtacg tcaacagag aggacacga gtccaattt tcagcagcg acgatcact cgcacgagc aaaggcccg acgtcgtga gaaagaagc cgacgccca agacacgga tattggcgc ccacgctgc ccgccctca actcgaaca acctgaacg cttacttgc cgcagagca cgacgctgc acccggaca acgtgcatc aattgtggc ccttccaga cattcagca actcgcgag gccatcgca tggtggcct tcccggagg agtccttcc aggagaact cgttcgcgc cgccaacga ccgaagacg aattcggtt cggtcgtag ttctgggta aaggaactc cggaaaact taaaattcg aatgggtca tttccgatt ggtcgaaat gctcgcaaa cctgcggcg gaaatggct ttcagatga gggcaattc gttgcattg ttcgactac ataatacga cacacggcg tcgatcata acttttgcg aggacgctg gcatgccaa ttccagcga cggaacgaa aatgcggac tgaacgact gtcccagat gggtgactt ccgaatgga cgccctgcg acaagtcca agtgcttcg attggcaca cagcaatgc agaggagat tggtcaagt gccagattg aagggaatg tcacggtgg cggagtcca agtgcaacg aagaggata agccgacgc acaggcagg agtgcgcca acgagaggt gtgtgggca agtggaagc tcggagctt ggtcagaat gctctgcgg attgcgaaa tgcaaggat tcaagtaca gaatcctgc aatgtgttt ggtatggca cgaaaaaac ctgcaggga ctgcctgta aagaccttc cccgtcctc cagtcatga agacttgta aaggacctc cgtgctcca aatcagagt cgcaatgca aagaccact ctatgtttt gttcgaacg tgaaaacga tgaacatgt gcaaaataa cgaggtacc aacagcaat gctgcaaga cgtgcaagg ccggttaga gttcgaaga gtctccgaa aaggcttaa attaatatt ttgagacgg tggagttat gttgtgtgg gcaaagcca agtgtgaag tgtgttcaa aagtgactt atatcatct attaaatct cttggacaa gtgctgcac ttagcaata gtagaagat aggaaatac gtttgaatc tcagcttgt gctgatctg atgtatatt aatgtactc gtcgtgttc agtggcgaa tttttgccc tttgatgga tgtttttgt gagagggta aaaatcgcg agtgagaac gacacattc aagactgat gttttttgt tacaattta tttgttgga tactatata catcccatg acattccat tattaccgg tattaaata ttaggtccg tcgtaacat gcaacggtt tcaaaggta gttaagtat aaaattttg tttttcgtg taaccgacc agaaatttt tgggttatt gcatactta aacctcaac acgtacctg cttttatta ttctgtaat aatacatgt atttttgta tt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
