Status
Please wait ...
Gene information
General information
TC033768
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009960
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11306364..11328738
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033768
    iBeetle screen iB_01635

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (41 dpi)
    Days post injection


    Pupal morphology
    dorsal thoracic muscles mostlynot present - Number of animals showing the phenotype: 9

    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects -
    Comment:
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033768 RA
      MFVVLVFKS KVPLIPSLA WSFCRGLLS CPEAHEFRE 
      VQCSAYNSI PYQGNLYEW SAHYNDSNP CALTCKGKP 
      ISESSNEDP VLVVVKLAD KVHDGTRCR LGSLDMCID 
      GECQRVGCD LRIGSTRQV DECGVCGGD GSSCSRPLY 
      HWTLTYMSL CSVTCGGGY KMSRPLCQN RVTGEEVEE 
      ELCNESQKP DSTIVECNT HNCPPKWHM GEWGPCSVS 
      CGGGSRLRQ VYCVEEANS TKIRVNDQK CTGHKPWFQ 
      EACNRMDCP AWIASSWSG CSVSCGEGT QVRVVECRD 
      SADKPSTLC PQKLKPIDS KPCSTGIQC PHHIDASEE 
      FLPGLYHTQ PLVQPYPPP PPAHAERLV GEQVVPSES 
      TFVPDEWGP CSVTCGEGV RKRQVHCKI FLEFSRTIA 
      KLPDKQCTG PKPIETEKC YMEPCSAER IGVDIKDDP 
      YRSDIKVAG GFPGKSYSW KEQGYTHCS ATCLGGVQE 
      LIVNCVRDD TQKVTSPYL CPIELKPEI LMRTCNDHP 
      CPPRWNYSD FSTCSQSCG IGIQTREVN CIHEVTQGG 
      GNTVIVPNN MCPQPPPPD RQYCNVLDC PVRWKVSEW 
      SRCSKPCGG GEKIRKVEC KQVMAQNHT VDRSPELCP 
      SPKPPDRKP CNTKSCIIE SDKPHIDVS NSSFIQHDF 
      KKKKIALKV GGSATVFIG TTVKIKCPV KRFNRTKIQ 
      WVKDKNFLP KSKKYKTSK KGALRVQNL TLRDSGRYT 
      CVAGRSSAS IQISVRPKP GEFLTSEEI QKQGKPDRS 
      ELISDVYVN REDTSPIFS SDDHSHEQR PDVVRKKPT 
      PKTRILAPT LPALNSNNL NAYLPQSTT LHPDNVHQL 
      WPFQTFSNS RGHRMVAFP EESFQENSF APPTTEDEF 
      GSVVVLGKG TPENLKFEW VISDWSKCS QTCGGNGFQ 
      MRAIRCIVR LHNTTHGVD HNFCEDAGM PIPATERKC 
      GLNDCPRWV TSEWTPCDK SKCFDWHTA MQRRLVKCQ 
      IEGNVTVAE SKCNEEDKP THRQECANE RCVGKWKLG 
      AWSECSADC EMQGFKYRI LQCVWYGTK KPAGTACKD 
      LPRPPVMKT CKGPPCSKS ESQCKDHSM FCSNVKTMN 
      MCKITRYQQ QCCKTCKAG
    CDS sequence
    • >TC033768 RA
      atgtttgtg gtgctcgtt ttcaagagc aaggttccg 
      ttgattccg tcactggcg tggtctttt tgccgtggg 
      cttctttcg tgcccagag gctcacgaa ttccgcgag 
      gtccagtgc tcagcatac aacagcatt ccctaccag 
      ggaaatctt tacgagtgg tcagcacat tacaatgac 
      tcgaacccg tgcgctctt acctgcaaa ggcaagccg 
      attagcgag tcaagtaat gaggatcct gtgcttgtt 
      gtggtaaag cttgcagat aaagttcat gacggaact 
      agatgcaga ctgggaagt ctggacatg tgtatcgat 
      ggagaatgt cagagagtg gggtgtgat ctgaggatt 
      ggctcgacg agacaagtg gatgaatgt ggagtttgt 
      ggcggagat ggatcgtca tgttcgagg cctttgtac 
      cactggact ctgacgtat atgtcgttg tgctcggtg 
      acatgtggc gggggctac aaaatgtca cgtccctta 
      tgtcagaac cgggtgact ggagaggaa gttgaggaa 
      gaactttgt aacgagtca caaaagccc gattcgacg 
      atagtcgag tgtaacacc cataactgc cctcccaag 
      tggcacatg ggtgagtgg ggcccgtgc tcagtgagc 
      tgtggtgga gggtcaaga cttcggcaa gtttattgc 
      gtggaagaa gccaacagt actaaaatt agggttaat 
      gatcagaag tgcaccgga cacaagccg tggtttcag 
      gaagcctgc aatcgaatg gattgtccg gcctggatt 
      gcatcgtcc tggtcagga tgctcggtg tcttgcggg 
      gaaggcacg caagtgagg gtggttgaa tgccgagat 
      tccgcagac aagcccagc acattgtgc cctcagaaa 
      cttaagcct attgacagc aagccctgt tcgacgggc 
      attcagtgt ccgcatcac atagatgcc agcgaggag 
      tttttgccc ggcctctat cacactcaa cctttggtc 
      cagccgtac cctccgccg cccccagct catgccgaa 
      cgactcgtc ggggagcag gttgtgccc tcggaaagc 
      acatttgtt cccgacgag tggggccct tgcagcgtg 
      acttgcggc gaaggcgtc cgcaaacgt caagtccac 
      tgcaagatc ttcctcgaa ttttcaaga actatcgcc 
      aaactccca gacaaacag tgtaccggt cctaaacca 
      atagagaca gaaaaatgc tacatggaa ccatgctca 
      gccgaacgc atcggggtc gatattaaa gacgacccc 
      tatcgatcg gacatcaaa gtcgccggg ggcttcccc 
      ggaaagtcc tactcgtgg aaggagcaa ggctacacc 
      cactgcagc gccacgtgt ctcggaggc gtccaagag 
      ctgatagtc aactgtgtc cgcgacgac acgcagaaa 
      gtcacctcc ccgtacttg tgccccatc gagctcaag 
      cccgaaata ctcatgagg acgtgcaac gaccacccg 
      tgcccccct cggtggaac tactccgat ttttcgact 
      tgttcgcaa agctgcggc atcgggatt cagaccagg 
      gaggtcaac tgcattcac gaggtgacc caaggcggg 
      ggcaacact gtaatagtc ccgaataac atgtgcccg 
      caaccgccg cccccagac cggcagtat tgcaacgtc 
      ctggactgc ccagtcagg tggaaagtg tcggaatgg 
      tccaggtgt agcaagcca tgtggcggt ggtgaaaaa 
      atcaggaaa gtggagtgt aagcaagtc atggcccaa 
      aaccacaca gtggacaga tcacccgag ctgtgcccc 
      agtcccaag ccacccgac cggaagcct tgtaacacc 
      aaaagttgc atcattgaa agtgacaag ccccacatt 
      gacgtttca aacagttcg ttcattcag cacgatttc 
      aagaagaag aaaattgcg ttgaaagtc ggaggctca 
      gctactgtt tttattgga accacggtt aaaatcaaa 
      tgcccggtc aagagattc aaccgcacg aaaatccag 
      tgggtcaaa gacaagaac ttcctgccc aagtccaag 
      aaatacaag acgtccaag aagggggcc ttgcgggtg 
      caaaatctg acgctccgc gacagcggc cgctacact 
      tgcgtggca ggccgttcc tccgccagt atccaaatt 
      tccgttcgc cccaaaccc ggcgaattc ctcacatcc 
      gaggagatc cagaagcag ggcaagccc gaccgcagc 
      gagctaata tccgacgtg tacgtcaac agagaggac 
      acgagtcca attttcagc agcgacgat cactcgcac 
      gagcaaagg cccgacgtc gtgagaaag aagccgacg 
      cccaagaca cggatattg gcgcccacg ctgcccgcc 
      ctcaactcg aacaacctg aacgcttac ttgccgcag 
      agcacgacg ctgcacccg gacaacgtg catcaattg 
      tggcccttc cagacattc agcaactcg cgaggccat 
      cgcatggtg gccttcccg gaggagtcc ttccaggag 
      aactcgttc gcgccgcca acgaccgaa gacgaattc 
      ggttcggtc gtagttctg ggtaaagga actccggaa 
      aacttaaaa ttcgaatgg gtcatttcc gattggtcg 
      aaatgctcg caaacctgc ggcggaaat ggctttcag 
      atgagggca attcgttgc attgttcga ctacataat 
      acgacacac ggcgtcgat cataacttt tgcgaggac 
      gctggcatg ccaattcca gcgacggaa cgaaaatgc 
      ggactgaac gactgtccc agatgggtg acttccgaa 
      tggacgccc tgcgacaag tccaagtgc ttcgattgg 
      cacacagca atgcagagg agattggtc aagtgccag 
      attgaaggg aatgtcacg gtggcggag tccaagtgc 
      aacgaagag gataagccg acgcacagg caggagtgc 
      gccaacgag aggtgtgtg ggcaagtgg aagctcgga 
      gcttggtca gaatgctct gcggattgc gaaatgcaa 
      ggattcaag tacagaatc ctgcaatgt gtttggtat 
      ggcacgaaa aaacctgca gggactgcc tgtaaagac 
      cttccccgt cctccagtc atgaagact tgtaaagga 
      cctccgtgc tccaaatca gagtcgcaa tgcaaagac 
      cactctatg ttttgttcg aacgtgaaa acgatgaac 
      atgtgcaaa ataacgagg taccaacag caatgctgc 
      aagacgtgc aaggccggt tag
    mRNA sequence
    • >TC033768 RA
      tgaaagttc cgcacatgt ttgtggtgc tcgttttca 
      agagcaagg ttccgttga ttccgtcac tggcgtggt 
      ctttttgcc gtgggcttc tttcgtgcc cagaggctc 
      acgaattcc gcgaggtcc agtgctcag catacaaca 
      gcattccct accagggaa atctttacg agtggtcag 
      cacattaca atgactcga acccgtgcg ctcttacct 
      gcaaaggca agccgatta gcgagtcaa gtaatgagg 
      atcctgtgc ttgttgtgg taaagcttg cagataaag 
      ttcatgacg gaactagat gcagactgg gaagtctgg 
      acatgtgta tcgatggag aatgtcaga gagtggggt 
      gtgatctga ggattggct cgacgagac aagtggatg 
      aatgtggag tttgtggcg gagatggat cgtcatgtt 
      cgaggcctt tgtaccact ggactctga cgtatatgt 
      cgttgtgct cggtgacat gtggcgggg gctacaaaa 
      tgtcacgtc ccttatgtc agaaccggg tgactggag 
      aggaagttg aggaagaac tttgtaacg agtcacaaa 
      agcccgatt cgacgatag tcgagtgta acacccata 
      actgccctc ccaagtggc acatgggtg agtggggcc 
      cgtgctcag tgagctgtg gtggagggt caagacttc 
      ggcaagttt attgcgtgg aagaagcca acagtacta 
      aaattaggg ttaatgatc agaagtgca ccggacaca 
      agccgtggt ttcaggaag cctgcaatc gaatggatt 
      gtccggcct ggattgcat cgtcctggt caggatgct 
      cggtgtctt gcggggaag gcacgcaag tgagggtgg 
      ttgaatgcc gagattccg cagacaagc ccagcacat 
      tgtgccctc agaaactta agcctattg acagcaagc 
      cctgttcga cgggcattc agtgtccgc atcacatag 
      atgccagcg aggagtttt tgcccggcc tctatcaca 
      ctcaacctt tggtccagc cgtaccctc cgccgcccc 
      cagctcatg ccgaacgac tcgtcgggg agcaggttg 
      tgccctcgg aaagcacat ttgttcccg acgagtggg 
      gcccttgca gcgtgactt gcggcgaag gcgtccgca 
      aacgtcaag tccactgca agatcttcc tcgaatttt 
      caagaacta tcgccaaac tcccagaca aacagtgta 
      ccggtccta aaccaatag agacagaaa aatgctaca 
      tggaaccat gctcagccg aacgcatcg gggtcgata 
      ttaaagacg acccctatc gatcggaca tcaaagtcg 
      ccgggggct tccccggaa agtcctact cgtggaagg 
      agcaaggct acacccact gcagcgcca cgtgtctcg 
      gaggcgtcc aagagctga tagtcaact gtgtccgcg 
      acgacacgc agaaagtca cctccccgt acttgtgcc 
      ccatcgagc tcaagcccg aaatactca tgaggacgt 
      gcaacgacc acccgtgcc cccctcggt ggaactact 
      ccgattttt cgacttgtt cgcaaagct gcggcatcg 
      ggattcaga ccagggagg tcaactgca ttcacgagg 
      tgacccaag gcgggggca acactgtaa tagtcccga 
      ataacatgt gcccgcaac cgccgcccc cagaccggc 
      agtattgca acgtcctgg actgcccag tcaggtgga 
      aagtgtcgg aatggtcca ggtgtagca agccatgtg 
      gcggtggtg aaaaaatca ggaaagtgg agtgtaagc 
      aagtcatgg cccaaaacc acacagtgg acagatcac 
      ccgagctgt gccccagtc ccaagccac ccgaccgga 
      agccttgta acaccaaaa gttgcatca ttgaaagtg 
      acaagcccc acattgacg tttcaaaca gttcgttca 
      ttcagcacg atttcaaga agaagaaaa ttgcgttga 
      aagtcggag gctcagcta ctgttttta ttggaacca 
      cggttaaaa tcaaatgcc cggtcaaga gattcaacc 
      gcacgaaaa tccagtggg tcaaagaca agaacttcc 
      tgcccaagt ccaagaaat acaagacgt ccaagaagg 
      gggccttgc gggtgcaaa atctgacgc tccgcgaca 
      gcggccgct acacttgcg tggcaggcc gttcctccg 
      ccagtatcc aaatttccg ttcgcccca aacccggcg 
      aattcctca catccgagg agatccaga agcagggca 
      agcccgacc gcagcgagc taatatccg acgtgtacg 
      tcaacagag aggacacga gtccaattt tcagcagcg 
      acgatcact cgcacgagc aaaggcccg acgtcgtga 
      gaaagaagc cgacgccca agacacgga tattggcgc 
      ccacgctgc ccgccctca actcgaaca acctgaacg 
      cttacttgc cgcagagca cgacgctgc acccggaca 
      acgtgcatc aattgtggc ccttccaga cattcagca 
      actcgcgag gccatcgca tggtggcct tcccggagg 
      agtccttcc aggagaact cgttcgcgc cgccaacga 
      ccgaagacg aattcggtt cggtcgtag ttctgggta 
      aaggaactc cggaaaact taaaattcg aatgggtca 
      tttccgatt ggtcgaaat gctcgcaaa cctgcggcg 
      gaaatggct ttcagatga gggcaattc gttgcattg 
      ttcgactac ataatacga cacacggcg tcgatcata 
      acttttgcg aggacgctg gcatgccaa ttccagcga 
      cggaacgaa aatgcggac tgaacgact gtcccagat 
      gggtgactt ccgaatgga cgccctgcg acaagtcca 
      agtgcttcg attggcaca cagcaatgc agaggagat 
      tggtcaagt gccagattg aagggaatg tcacggtgg 
      cggagtcca agtgcaacg aagaggata agccgacgc 
      acaggcagg agtgcgcca acgagaggt gtgtgggca 
      agtggaagc tcggagctt ggtcagaat gctctgcgg 
      attgcgaaa tgcaaggat tcaagtaca gaatcctgc 
      aatgtgttt ggtatggca cgaaaaaac ctgcaggga 
      ctgcctgta aagaccttc cccgtcctc cagtcatga 
      agacttgta aaggacctc cgtgctcca aatcagagt 
      cgcaatgca aagaccact ctatgtttt gttcgaacg 
      tgaaaacga tgaacatgt gcaaaataa cgaggtacc 
      aacagcaat gctgcaaga cgtgcaagg ccggttaga 
      gttcgaaga gtctccgaa aaggcttaa attaatatt 
      ttgagacgg tggagttat gttgtgtgg gcaaagcca 
      agtgtgaag tgtgttcaa aagtgactt atatcatct 
      attaaatct cttggacaa gtgctgcac ttagcaata 
      gtagaagat aggaaatac gtttgaatc tcagcttgt 
      gctgatctg atgtatatt aatgtactc gtcgtgttc 
      agtggcgaa tttttgccc tttgatgga tgtttttgt 
      gagagggta aaaatcgcg agtgagaac gacacattc 
      aagactgat gttttttgt tacaattta tttgttgga 
      tactatata catcccatg acattccat tattaccgg 
      tattaaata ttaggtccg tcgtaacat gcaacggtt 
      tcaaaggta gttaagtat aaaattttg tttttcgtg 
      taaccgacc agaaatttt tgggttatt gcatactta 
      aacctcaac acgtacctg cttttatta ttctgtaat 
      aatacatgt atttttgta tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY