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Gene information
General information
TC009901 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 5817466..5821504
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009901
    iBeetle screen iB_01625
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009901 RA
      MGAEGLSPR DPSSYSRPE DVAVTNIFL NLTVDFDKK 
      ILHGNVDLD VSKINKNAT ELILDAKDL KISKVIDDN 
      TKQELKFVI SQPQEEFGS KLSVELPKQ GNDTYKISI 
      FYENSPNAS GLQWLTPEQ TAGKKHPYL FSQFQAIHA 
      RSVLPCQDT PEVKTKYQA TISAPSNLT VLMSAIPSK 
      TEVSGAFKR SHFSQDVPM PAYLIAIAV GALESRKIG 
      PRSHVWAEK EIIEDCAYE FAETEHQLK TAEEICGPY 
      VWGIYDLLV LPPSFAFGG MENPCLTFV TPTLLAGDR 
      SLANVVAHE IAHSWTGNL ITNRNFEHF WLNEGFTVF 
      VERKIKARL TSPAHQDFD AYCRLSELK EAVKLLGEN 
      SPLTKLVVD LKGVHPDDA FSVVPYEKG QTFLRYLEE 
      VVGGPAQFE PFLREYFNT FKYKSIDTN DFKSFFEKY 
      FANKAAIKS VDWNTWLYG TGMPPVIPN YNTSLATNC 
      DKLMKQFVE WDEKAACPV ETTHIKNLG ADEIIYLLQ 
      EIWSGKPLS IPKLEQLDK LFEFSKVKN SEVKFRWLR 
      IGLKAHWMK SVEPTLKWI NEVGRMKFV RPLYRDLYA 
      WEEVRSKAI ENFKLNKNS MMHVVAYTV SKDLHLTS
    CDS sequence
    • >TC009901 RA
      atgggtgca gaaggctta agtccaaga gaccctagc 
      tcatactct agaccagag gatgttgct gtaacaaat 
      attttcctt aatttaact gtggatttt gataaaaaa 
      attctccac ggcaatgtg gacctggac gtctctaaa 
      attaataaa aatgcaacc gaactgatt ttagatgca 
      aaagacttg aaaatatca aaagtaatt gacgacaat 
      accaaacaa gaactgaaa tttgtaata tctcaacca 
      caggaggag tttggttcg aaactttcg gttgaatta 
      cccaagcag ggaaatgat acttataaa atttcgatt 
      ttttatgaa aatagccca aatgctagc ggtttgcag 
      tggctaacc cctgaacaa acagcaggg aaaaagcac 
      ccttatctt ttcagccaa ttccaggcc attcatgct 
      aggtctgtt ctcccctgt caggacact cccgaagtc 
      aagacaaaa taccaagct accatcagc gctccttca 
      aatttaaca gtcttaatg tcggccatc ccttccaag 
      actgaagtt tccggcgcc ttcaagcgt tcgcatttc 
      agccaagac gtcccaatg cccgcctac ctcatagcc 
      atagcagta ggtgctcta gaatcgcgc aaaatcggc 
      cccagatcg cacgtttgg gccgaaaaa gagatcatc 
      gaggactgc gcctacgaa tttgccgag actgagcac 
      cagctgaag actgccgaa gagatctgc gggccgtac 
      gtgtggggc atttacgac ttgcttgtc cttcccccc 
      agttttgca tttggagga atggaaaat ccgtgtttg 
      actttcgtt actcccact ttactcgcg ggcgatagg 
      tctttggct aatgtggtg gcgcatgaa atagcacac 
      agctggacg gggaatcta atcacgaat cgcaatttt 
      gagcatttc tggctgaat gaagggttt acggttttt 
      gttgaacgg aagattaag gcgcggttg acgtcaccg 
      gcgcatcag gattttgac gcctattgc aggctgagt 
      gagttaaaa gaggcggtg aagttgctt ggggaaaac 
      agccctttg acaaaactg gttgtcgac ttgaagggg 
      gttcacccc gatgatgcg ttttcggtg gttccctac 
      gaaaaaggc caaactttt ttgcgctac cttgaagaa 
      gtagttggc gggccagct caattcgaa ccattcctc 
      agagagtac ttcaacact tttaaatac aaatcgatc 
      gacaccaac gactttaaa tcatttttc gaaaaatat 
      ttcgcaaac aaggcggcc atcaaatcg gttgattgg 
      aacacctgg ctttacggc accggaatg cctccagtg 
      atccccaac tacaacacg tccctggct accaactgt 
      gacaaactt atgaaacag tttgtcgag tgggatgaa 
      aaagctgcc tgtcctgtt gaaaccacg cacattaag 
      aatcttggt gccgatgaa ataatttac cttctgcaa 
      gaaatatgg agcggaaaa ccgttgtct attcccaaa 
      cttgagcaa ctggacaag ttgttcgag tttagtaaa 
      gtgaaaaat tccgaagtg aagttcaga tggttgaga 
      attggttta aaagcgcac tggatgaag agtgttgaa 
      ccgactctg aagtggatc aatgaagtg ggaagaatg 
      aaatttgtg cgccccctg tacagggat ttgtacgct 
      tgggaggaa gtgagaagc aaggccatc gaaaacttc 
      aagttgaat aaaaattca atgatgcac gtggtggct 
      tatactgtt tctaaggac ttgcattta acgtcttaa
    mRNA sequence
    • >TC009901 RA
      acccaatta aaaataacc caaaaaaat atattaaat 
      tataagacc aataatacg caaccaaga tcttaaaat 
      gttcccaaa attgcaaaa caggaaaaa ttaagaaaa 
      atgaagaat gttcctgcg ttacgttgc gctacgggc 
      caccgtaac gtagaagtt ctcactttt tgacacatt 
      tttctgtca cagaattcc ggtaatgcc caatttggc 
      ataatttaa ttaaattgg taatttagc ttttttgcg 
      attttaaat tacttaact aaactcttg aaggtgctt 
      gagtattct tcaatgatt atttgcaac agataaaat 
      caaacttta aagtactga ttaatttag gttaggtgc 
      aagaattac aagtggatt tttcctata aaaggtgaa 
      caaggaaag agcaggagt gataatggg tgcagaagg 
      cttaagtcc aagagaccc tagctcata ctctagacc 
      agaggatgt tgctgtaac aaatatttt ccttaattt 
      aactgtgga ttttgataa aaaaattct ccacggcaa 
      tgtggacct ggacgtctc taaaattaa taaaaatgc 
      aaccgaact gattttaga tgcaaaaga cttgaaaat 
      atcaaaagt aattgacga caataccaa acaagaact 
      gaaatttgt aatatctca accacagga ggagtttgg 
      ttcgaaact ttcggttga attacccaa gcagggaaa 
      tgatactta taaaatttc gatttttta tgaaaatag 
      cccaaatgc tagcggttt gcagtggct aacccctga 
      acaaacagc agggaaaaa gcaccctta tcttttcag 
      ccaattcca ggccattca tgctaggtc tgttctccc 
      ctgtcagga cactcccga agtcaagac aaaatacca 
      agctaccat cagcgctcc ttcaaattt aacagtctt 
      aatgtcggc catcccttc caagactga agtttccgg 
      cgccttcaa gcgttcgca tttcagcca agacgtccc 
      aatgcccgc ctacctcat agccatagc agtaggtgc 
      tctagaatc gcgcaaaat cggccccag atcgcacgt 
      ttgggccga aaaagagat catcgagga ctgcgccta 
      cgaatttgc cgagactga gcaccagct gaagactgc 
      cgaagagat ctgcgggcc gtacgtgtg gggcattta 
      cgacttgct tgtccttcc ccccagttt tgcatttgg 
      aggaatgga aaatccgtg tttgacttt cgttactcc 
      cactttact cgcgggcga taggtcttt ggctaatgt 
      ggtggcgca tgaaatagc acacagctg gacggggaa 
      tctaatcac gaatcgcaa ttttgagca tttctggct 
      gaatgaagg gtttacggt ttttgttga acggaagat 
      taaggcgcg gttgacgtc accggcgca tcaggattt 
      tgacgccta ttgcaggct gagtgagtt aaaagaggc 
      ggtgaagtt gcttgggga aaacagccc tttgacaaa 
      actggttgt cgacttgaa gggggttca ccccgatga 
      tgcgttttc ggtggttcc ctacgaaaa aggccaaac 
      ttttttgcg ctaccttga agaagtagt tggcgggcc 
      agctcaatt cgaaccatt cctcagaga gtacttcaa 
      cacttttaa atacaaatc gatcgacac caacgactt 
      taaatcatt tttcgaaaa atatttcgc aaacaaggc 
      ggccatcaa atcggttga ttggaacac ctggcttta 
      cggcaccgg aatgcctcc agtgatccc caactacaa 
      cacgtccct ggctaccaa ctgtgacaa acttatgaa 
      acagtttgt cgagtggga tgaaaaagc tgcctgtcc 
      tgttgaaac cacgcacat taagaatct tggtgccga 
      tgaaataat ttaccttct gcaagaaat atggagcgg 
      aaaaccgtt gtctattcc caaacttga gcaactgga 
      caagttgtt cgagtttag taaagtgaa aaattccga 
      agtgaagtt cagatggtt gagaattgg tttaaaagc 
      gcactggat gaagagtgt tgaaccgac tctgaagtg 
      gatcaatga agtgggaag aatgaaatt tgtgcgccc 
      cctgtacag ggatttgta cgcttggga ggaagtgag 
      aagcaaggc catcgaaaa cttcaagtt gaataaaaa 
      ttcaatgat gcacgtggt ggcttatac tgtttctaa 
      ggacttgca tttaacgtc ttaaaactg tgttaaata 
      aatttttac gcaatat