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Gene information
General information
TC009874 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 12818361..12819955
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009874
    iBeetle screen iB_01622

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: Fluorescence strongly decreased, but larvae were still alive
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009874 RA
      MKCHYEILN VPRDAELPE IKTAYRKAA LKWHPDKNL 
NDTEFAKEQ FQMVQQAYD VLSDPQERA WYDKHREQI 
LRGSNSEFQ DNCLDLFQY FTTTCFRGY GDDEKGFYT 
VYRNVFDQI IKEDLEFID DKEEFCDIP RFGDSKSDY 
DEVVGPFYS YWSSYCTKK SYVWLDPYN INETRDRRV 
LKVIEKENK KVRQKAKKE RNDEIRNLV AFVRKRDKR 
VQEHTKLLE AKILENRQK QEELSKQKR LERKKQLNE 
STQAEWSKF DNVKSELEE IEKNLAEQF GEEFSNSED 
EEDEEVNDL YCVACNKVF KTPKALENH ESSKKHREN 
VELLKEIML EEEDGECLD EELDNSQIE DLTENEDSE 
DSAEREKKA KKKNKKAKN ILTVNNDEE VDVTKIEES 
DDNFDFDST KKQKKKKKQ NKRANEPKN EENQNTEPI 
TKSIKKKQS KKEKQACSI EEIDTNHCC VSCKSAFPS 
KNKLFEHLK STGHGVFLP KNVKKKKNA RED
    CDS sequence
    • >TC009874 RA
      atgaagtgt cattacgaa attctcaat gttcctcga 
gacgccgag ctgccagaa attaaaact gcgtacaga 
aaggccgct ctgaagtgg caccccgac aaaaacctc 
aacgacact gagtttgct aaagagcag ttccagatg 
gtacagcag gcttacgac gtgctcagc gatccccaa 
gagcgggct tggtacgac aagcaccgc gagcaaatt 
ttgcgcggc tccaattcc gagtttcaa gacaactgt 
cttgacctg ttccagtat ttcaccact acgtgtttc 
agagggtat ggggatgat gaaaaaggc ttttacacg 
gtttatcgc aatgtgttt gaccaaatc atcaaggag 
gatcttgag tttattgac gacaaggaa gagttctgt 
gacatacca aggtttggt gattcaaag agtgactac 
gatgaagtt gtcggtccg ttttactcc tactggtcg 
agttattgc actaagaaa agctacgtt tggctggat 
ccgtacaat attaacgaa acgcgtgat agaagggta 
ttgaaagta atagagaaa gaaaataaa aaagttaga 
cagaaagcc aagaaagaa aggaatgat gaaatcaga 
aatcttgtt gcttttgtg aggaaaagg gacaaacgg 
gttcaagaa cacactaag cttttggag gccaaaata 
ttggagaat cgtcagaaa caggaagag ttgagtaag 
caaaaaaga cttgagagg aaaaaacag ttgaatgaa 
tcgactcaa gccgaatgg tccaaattt gataacgta 
aaatctgag ttggaagaa atcgagaag aatctagct 
gaacagttc ggtgaagaa ttttcaaat tctgaagac 
gaggaagat gaagaagtt aatgatctc tattgtgta 
gcgtgcaat aaggtgttc aaaacccct aaagcttta 
gaaaatcac gaatcaagc aaaaagcac cgagaaaac 
gtagaatta cttaaagaa attatgtta gaagaagag 
gatggagag tgtctagat gaagaatta gataattca 
caaattgaa gatctcact gaaaatgag gactcggaa 
gattccgca gaaagagag aagaaagca aaaaagaaa 
aacaaaaag gctaagaac attctcact gttaataat 
gacgaagaa gttgacgta acgaaaatt gaggaatca 
gacgataat ttcgacttt gactctact aaaaaacag 
aagaagaaa aagaagcaa aataagcgt gcgaatgaa 
ccgaaaaat gaagaaaat caaaacaca gaacctatt 
actaagagc atcaaaaag aagcaaagt aagaaggaa 
aaacaagct tgcagtatt gaagaaata gacaccaac 
cactgttgt gtcagctgt aaatccgcc ttcccatca 
aaaaataaa ttatttgaa catctaaaa agcaccgga 
catggagtt tttctgccc aaaaacgta aagaaaaag 
aaaaacgcg cgagaagat tag
    mRNA sequence
    • >TC009874 RA
      gttaggtta cggcgacca cattttttg cgaaaataa 
aaaacttca attaaaaat gaagtgtca ttacgaaat 
tctcaatgt tcctcgaga cgccgagct gccagaaat 
taaaactgc gtacagaaa ggccgctct gaagtggca 
ccccgacaa aaacctcaa cgacactga gtttgctaa 
agagcagtt ccagatggt acagcaggc ttacgacgt 
gctcagcga tccccaaga gcgggcttg gtacgacaa 
gcaccgcga gcaaatttt gcgcggctc caattccga 
gtttcaaga caactgtct tgacctgtt ccagtattt 
caccactac gtgtttcag agggtatgg ggatgatga 
aaaaggctt ttacacggt ttatcgcaa tgtgtttga 
ccaaatcat caaggagga tcttgagtt tattgacga 
caaggaaga gttctgtga cataccaag gtttggtga 
ttcaaagag tgactacga tgaagttgt cggtccgtt 
ttactccta ctggtcgag ttattgcac taagaaaag 
ctacgtttg gctggatcc gtacaatat taacgaaac 
gcgtgatag aagggtatt gaaagtaat agagaaaga 
aaataaaaa agttagaca gaaagccaa gaaagaaag 
gaatgatga aatcagaaa tcttgttgc ttttgtgag 
gaaaaggga caaacgggt tcaagaaca cactaagct 
tttggaggc caaaatatt ggagaatcg tcagaaaca 
ggaagagtt gagtaagca aaaaagact tgagaggaa 
aaaacagtt gaatgaatc gactcaagc cgaatggtc 
caaatttga taacgtaaa atctgagtt ggaagaaat 
cgagaagaa tctagctga acagttcgg tgaagaatt 
ttcaaattc tgaagacga ggaagatga agaagttaa 
tgatctcta ttgtgtagc gtgcaataa ggtgttcaa 
aacccctaa agctttaga aaatcacga atcaagcaa 
aaagcaccg agaaaacgt agaattact taaagaaat 
tatgttaga agaagagga tggagagtg tctagatga 
agaattaga taattcaca aattgaaga tctcactga 
aaatgagga ctcggaaga ttccgcaga aagagagaa 
gaaagcaaa aaagaaaaa caaaaaggc taagaacat 
tctcactgt taataatga cgaagaagt tgacgtaac 
gaaaattga ggaatcaga cgataattt cgactttga 
ctctactaa aaaacagaa gaagaaaaa gaagcaaaa 
taagcgtgc gaatgaacc gaaaaatga agaaaatca 
aaacacaga acctattac taagagcat caaaaagaa 
gcaaagtaa gaaggaaaa acaagcttg cagtattga 
agaaataga caccaacca ctgttgtgt cagctgtaa 
atccgcctt cccatcaaa aaataaatt atttgaaca 
tctaaaaag caccggaca tggagtttt tctgcccaa 
aaacgtaaa gaaaaagaa aaacgcgcg agaagatta 
gtctaattt aaattattg ttctatagt attaaactg 
tgaaataaa ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY