Status
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Gene information
General information
TC033720
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009856,TC009854
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9157746..9208510
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033720
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01620

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033720 RA
      MASDEDCST TFTYPIMDD ATSSPTTAN HYSNCLSPS 
      SSPSPLLPG QYQLTPRYE YQDQDMIPA SQVLPQVLP 
      KSQEMWGDM SQGIYADYR YETNRFNND YIRQSYAET 
      MPVFTQTRT NFVPKIGAQ GQKVPKEAR IRRPMNAFM 
      VWAKVERKK LADENPDLH NADLSKMLG KKWRSLTPQ 
      DRRPFVEEA ERLRVIHMT EHPNYKYRP RRRKHNKQR 
      ATSGAGGPR VGTSLPSPS LPNMSPRYA GYIPNASLS 
      PNIQQQNSY GMDFPSPGT ADFAAQDKR YSPDTFKYH 
      NQYNYVGYQ NYQKSPYSI QTPDASPSH SPEPKKGPN 
      SPQDCSKDS PREDASLPT PELSPMEHE QFEEKRVSS 
      IQHTSNLTN PAQQSYNTR VQNYRPNST NYTNIQPIT 
      SVPMANGMY VMSANKSSV EQGHVVTGT FYPPVATSQ 
      DQQLLGTNQ NNLNTVANN TNLHYYNAS MQQYYPSTN 
      TGYDQDQTK PDTFLTYQN PLKSDVVNE NINSYKSGG 
      LDESYQPQY SSSTLVQSY MGQEERSDV DSDVDTREF 
      DKYLKFGST ESNMIDSNH NYHRNDNIN SNVTYNFQA 
      QHTSVILPN TSVKPEPLL GYPEVYDIG QNGVVAKND 
      DDFSEILAD VRKTCYSN
    CDS sequence
    • >TC033720 RA
      atggcctcc gacgaagac tgtagtact accttcacg 
      taccctata atggacgac gcgacttcg tctcccaca 
      actgccaac cactacagc aattgcttg tccccgtcc 
      agctccccg tcgcccctt ttgccgggg cagtaccag 
      ctcaccccc aggtacgag taccaagac caagacatg 
      atccctgcg tcgcaggtg cttccccaa gtcctgcca 
      aaaagccag gagatgtgg ggtgacatg agtcagggg 
      atttacgcc gactaccgg tacgaaacg aaccgattt 
      aacaacgat tacattcgg caaagttac gcggaaact 
      atgcccgtg tttacgcaa actaggact aattttgtg 
      ccgaaaata ggtgcacaa ggacaaaaa gtgcccaaa 
      gaagcgagg attaggcgg ccgatgaac gcgttcatg 
      gtgtgggcc aaagtggaa aggaagaaa ttggccgac 
      gaaaatccg gatttgcat aatgccgat ttgagcaaa 
      atgttgggc aagaagtgg cgctctttg acgccgcaa 
      gacagacgg cctttcgtc gaagaagcc gaacgactg 
      agagtgatc cacatgact gaacacccc aactacaaa 
      tacagaccg cgacggcgg aagcacaac aagcagagg 
      gccacttcc ggtgccgga ggcccccgg gtcggaacc 
      tccttaccc agccccagt ctgcccaac atgtcgcca 
      aggtacgcg ggctacatt cccaacgcg agtctttcg 
      cccaacatt cagcagcag aacagctac ggaatggac 
      ttccctagt cccggaact gccgacttc gcagcgcag 
      gacaagaga tacagtccg gatactttc aagtaccac 
      aaccagtac aactatgtg gggtaccag aactaccag 
      aagagtccc tattcgatt caaacgcca gatgcgtct 
      ccctcacac agtcccgag ccgaagaaa gggccgaat 
      agtccgcag gattgtagc aaagacagt ccgcgggaa 
      gatgcgtcc ctccctaca ccggaactg agtccaatg 
      gagcacgaa cagttcgag gagaaaaga gtgtcgagt 
      atacaacat acaagtaac ttgactaac ccggcgcag 
      cagagttac aacacaaga gtacaaaac tacagacct 
      aatagtacc aactacacc aacatccaa cccatcact 
      tcggtacca atggcgaac gggatgtac gtgatgagt 
      gctaataag agctcagtg gagcagggg catgtggtc 
      actgggacg ttttaccca cccgtggct acgtcccag 
      gaccaacaa ctgctgggt acgaaccaa aacaatctc 
      aacactgtt gccaacaac acgaatttg cattactac 
      aacgcgtcc atgcaacag tactacccc agtaccaac 
      accgggtac gaccaagac caaaccaag ccagacacg 
      ttcctaact tatcaaaat cctctcaaa tcggacgtc 
      gtgaacgaa aatatcaac agttacaag tccgggggc 
      ttggacgag tcgtaccaa ccgcagtac tccagcagc 
      accctcgtg cagtcgtac atgggtcag gaggagcga 
      agcgacgtc gacagcgat gtggacact cgagagttc 
      gacaagtac ctcaagttt ggcagtacc gagtcgaat 
      atgatcgac tcgaatcac aactaccac aggaacgac 
      aatatcaat tcgaacgtg acttacaac ttccaagcc 
      cagcatacg tctgtgatt ttgcccaac acgagtgtc 
      aagcccgag ccgctgttg ggctacccc gaggtgtac 
      gatatcggt cagaacggc gtcgtcgcg aagaacgat 
      gacgatttt agtgagatt ctggcggac gtccggaag 
      acgtgctac agtaactga
    mRNA sequence
    • >TC033720 RA
      gccgtttcg gttcttcaa aaattgctt ccgtttaca 
      tatatgata aaagtcttt gtgcctttg tttctatta 
      actaaattt ctaaagaca agaaaaatt tgagtagat 
      agatcgaat agtgcttag ttaagctga aaaggtgct 
      ttcctgaaa gagtgtttt tcgagctta gaggagagc 
      tttggtgtg agtactcct tggaagtgg cataccttt 
      cagcaaacg tgatcggca atagtaaat gctagcaca 
      agccaataa aaatgtaac ctatgcatt agttattgt 
      tccagcggc tgtcataaa acgtccgtg gtatatttt 
      tagtgtagt atgaaacct gaaactgaa aacgacacc 
      gaactagcg tccatgtag tacaagcaa cggccacgc 
      gaggctcga aacacgcgc gctgcacgc aggtaaacc 
      ttaccttta ccctaccta ccaggtatg gcgggtaca 
      cagcccact gttcagtac cacggaacg cgagtacga 
      gtttgcacg cgtgtctgt ccctccagc gaggcccgt 
      cggaagcaa caagtgtgc atagatgtg aattcgaca 
      gtcggcatc gtcgacgat cgctctgtt ggtgtcaaa 
      tgcggcaaa agcgacggc cgtcggcga tgtagcctc 
      gagtgatga cgggtccaa gtgatcgat tggtgctgc 
      ggtgacact ccgggttaa gtgtcttta atgtaaaca 
      ggaggattt tgtttgctt gttaaatag gcgagcaaa 
      cagcagttt cttaatcaa accgtacca cgtcggaga 
      gcaaatcgt agaatgtag caatcgaaa ctgacatat 
      caaatgaag tggtgataa agtttcgtt tcaaagaca 
      ggtgacttc gttacgtga tcaattaac gataaaaaa 
      atggcctcc gacgaagac tgtagtact accttcacg 
      taccctata atggacgac gcgacttcg tctcccaca 
      actgccaac cactacagc aattgcttg tccccgtcc 
      agctccccg tcgcccctt ttgccgggg cagtaccag 
      ctcaccccc aggtacgag taccaagac caagacatg 
      atccctgcg tcgcaggtg cttccccaa gtcctgcca 
      aaaagccag gagatgtgg ggtgacatg agtcagggg 
      atttacgcc gactaccgg tacgaaacg aaccgattt 
      aacaacgat tacattcgg caaagttac gcggaaact 
      atgcccgtg tttacgcaa actaggact aattttgtg 
      ccgaaaata ggtgcacaa ggacaaaaa gtgcccaaa 
      gaagcgagg attaggcgg ccgatgaac gcgttcatg 
      gtgtgggcc aaagtggaa aggaagaaa ttggccgac 
      gaaaatccg gatttgcat aatgccgat ttgagcaaa 
      atgttgggc aagaagtgg cgctctttg acgccgcaa 
      gacagacgg cctttcgtc gaagaagcc gaacgactg 
      agagtgatc cacatgact gaacacccc aactacaaa 
      tacagaccg cgacggcgg aagcacaac aagcagagg 
      gccacttcc ggtgccgga ggcccccgg gtcggaacc 
      tccttaccc agccccagt ctgcccaac atgtcgcca 
      aggtacgcg ggctacatt cccaacgcg agtctttcg 
      cccaacatt cagcagcag aacagctac ggaatggac 
      ttccctagt cccggaact gccgacttc gcagcgcag 
      gacaagaga tacagtccg gatactttc aagtaccac 
      aaccagtac aactatgtg gggtaccag aactaccag 
      aagagtccc tattcgatt caaacgcca gatgcgtct 
      ccctcacac agtcccgag ccgaagaaa gggccgaat 
      agtccgcag gattgtagc aaagacagt ccgcgggaa 
      gatgcgtcc ctccctaca ccggaactg agtccaatg 
      gagcacgaa cagttcgag gagaaaaga gtgtcgagt 
      atacaacat acaagtaac ttgactaac ccggcgcag 
      cagagttac aacacaaga gtacaaaac tacagacct 
      aatagtacc aactacacc aacatccaa cccatcact 
      tcggtacca atggcgaac gggatgtac gtgatgagt 
      gctaataag agctcagtg gagcagggg catgtggtc 
      actgggacg ttttaccca cccgtggct acgtcccag 
      gaccaacaa ctgctgggt acgaaccaa aacaatctc 
      aacactgtt gccaacaac acgaatttg cattactac 
      aacgcgtcc atgcaacag tactacccc agtaccaac 
      accgggtac gaccaagac caaaccaag ccagacacg 
      ttcctaact tatcaaaat cctctcaaa tcggacgtc 
      gtgaacgaa aatatcaac agttacaag tccgggggc 
      ttggacgag tcgtaccaa ccgcagtac tccagcagc 
      accctcgtg cagtcgtac atgggtcag gaggagcga 
      agcgacgtc gacagcgat gtggacact cgagagttc 
      gacaagtac ctcaagttt ggcagtacc gagtcgaat 
      atgatcgac tcgaatcac aactaccac aggaacgac 
      aatatcaat tcgaacgtg acttacaac ttccaagcc 
      cagcatacg tctgtgatt ttgcccaac acgagtgtc 
      aagcccgag ccgctgttg ggctacccc gaggtgtac 
      gatatcggt cagaacggc gtcgtcgcg aagaacgat 
      gacgatttt agtgagatt ctggcggac gtccggaag 
      acgtgctac agtaactga cggtgcaat ttgagagtt 
      tagttttat tcgaattgt tgttttatg gccagttgt 
      tgtcgagtt gttgacgtc aaggagatg atttaaccc 
      aaaagtttc ggattagag caaattcca ctgtacaca 
      agtgtggtg tttaatttt ccatttcgg attaggttg 
      ttaaattta cggttttgt aataatttt agtggtgcg 
      caaacagat gctttaaat actccgacg agatctttg 
      ttctctttg tgttaattg ttactttcg cagagtgcc 
      ttatcatga tattatata caaagttgg tgtaaattt 
      tcgcctaat tttagtgta aattattct atgcataga 
      gatgattat atgttttgt acaatatta tttaagact 
      ggctatttt tgtatcaag tcgagacaa taaataaac 
      aatgacaat cagcgcgag acacgtcac ttaatatta 
      ttatgaaaa cttttatct cagtgtttt accaatatt 
      tgttcacac aaacttaaa taggatcta aggttaagc 
      tgtttatat acctattta attatgtga aagagattt 
      cttacaaaa aaccaaagt ttgtaaatt atgtatgat 
      agtactcta gttatcggc tattcaatt gtaaatacc 
      tagttatta cgtcatcat tgggaaata tatcattat 
      ttaatta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY