| General information | |||
| TC033720
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
scaffold_seqid49: 2490399..2541163 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC033720 | ||
| General information | ||||
| iB_01620 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC033720 RA
MASDEDCST TFTYPIMDD ATSSPTTAN HYSNCLSPS SSPSPLLPG QYQLTPRYE YQDQDMIPA SQVLPQVLP KSQEMWGDM SQGIYADYR YETNRFNND YIRQSYAET MPVFTQTRT NFVPKIGAQ GQKVPKEAR IRRPMNAFM VWAKVERKK LADENPDLH NADLSKMLG KKWRSLTPQ DRRPFVEEA ERLRVIHMT EHPNYKYRP RRRKHNKQR ATSGAGGPR VGTSLPSPS LPNMSPRYA GYIPNASLS PNIQQQNSY GMDFPSPGT ADFAAQDKR YSPDTFKYH NQYNYVGYQ NYQKSPYSI QTPDASPSH SPEPKKGPN SPQDCSKDS PREDASLPT PELSPMEHE QFEEKRVSS IQHTSNLTN PAQQSYNTR VQNYRPNST NYTNIQPIT SVPMANGMY VMSANKSSV EQGHVVTGT FYPPVATSQ DQQLLGTNQ NNLNTVANN TNLHYYNAS MQQYYPSTN TGYDQDQTK PDTFLTYQN PLKSDVVNE NINSYKSGG LDESYQPQY SSSTLVQSY MGQEERSDV DSDVDTREF DKYLKFGST ESNMIDSNH NYHRNDNIN SNVTYNFQA QHTSVILPN TSVKPEPLL GYPEVYDIG QNGVVAKND DDFSEILAD VRKTCYSN
- >TC033720 RA
atggcctcc gacgaagac tgtagtact accttcacg taccctata atggacgac gcgacttcg tctcccaca actgccaac cactacagc aattgcttg tccccgtcc agctccccg tcgcccctt ttgccgggg cagtaccag ctcaccccc aggtacgag taccaagac caagacatg atccctgcg tcgcaggtg cttccccaa gtcctgcca aaaagccag gagatgtgg ggtgacatg agtcagggg atttacgcc gactaccgg tacgaaacg aaccgattt aacaacgat tacattcgg caaagttac gcggaaact atgcccgtg tttacgcaa actaggact aattttgtg ccgaaaata ggtgcacaa ggacaaaaa gtgcccaaa gaagcgagg attaggcgg ccgatgaac gcgttcatg gtgtgggcc aaagtggaa aggaagaaa ttggccgac gaaaatccg gatttgcat aatgccgat ttgagcaaa atgttgggc aagaagtgg cgctctttg acgccgcaa gacagacgg cctttcgtc gaagaagcc gaacgactg agagtgatc cacatgact gaacacccc aactacaaa tacagaccg cgacggcgg aagcacaac aagcagagg gccacttcc ggtgccgga ggcccccgg gtcggaacc tccttaccc agccccagt ctgcccaac atgtcgcca aggtacgcg ggctacatt cccaacgcg agtctttcg cccaacatt cagcagcag aacagctac ggaatggac ttccctagt cccggaact gccgacttc gcagcgcag gacaagaga tacagtccg gatactttc aagtaccac aaccagtac aactatgtg gggtaccag aactaccag aagagtccc tattcgatt caaacgcca gatgcgtct ccctcacac agtcccgag ccgaagaaa gggccgaat agtccgcag gattgtagc aaagacagt ccgcgggaa gatgcgtcc ctccctaca ccggaactg agtccaatg gagcacgaa cagttcgag gagaaaaga gtgtcgagt atacaacat acaagtaac ttgactaac ccggcgcag cagagttac aacacaaga gtacaaaac tacagacct aatagtacc aactacacc aacatccaa cccatcact tcggtacca atggcgaac gggatgtac gtgatgagt gctaataag agctcagtg gagcagggg catgtggtc actgggacg ttttaccca cccgtggct acgtcccag gaccaacaa ctgctgggt acgaaccaa aacaatctc aacactgtt gccaacaac acgaatttg cattactac aacgcgtcc atgcaacag tactacccc agtaccaac accgggtac gaccaagac caaaccaag ccagacacg ttcctaact tatcaaaat cctctcaaa tcggacgtc gtgaacgaa aatatcaac agttacaag tccgggggc ttggacgag tcgtaccaa ccgcagtac tccagcagc accctcgtg cagtcgtac atgggtcag gaggagcga agcgacgtc gacagcgat gtggacact cgagagttc gacaagtac ctcaagttt ggcagtacc gagtcgaat atgatcgac tcgaatcac aactaccac aggaacgac aatatcaat tcgaacgtg acttacaac ttccaagcc cagcatacg tctgtgatt ttgcccaac acgagtgtc aagcccgag ccgctgttg ggctacccc gaggtgtac gatatcggt cagaacggc gtcgtcgcg aagaacgat gacgatttt agtgagatt ctggcggac gtccggaag acgtgctac agtaactga
- >TC033720 RA
gccgtttcg gttcttcaa aaattgctt ccgtttaca tatatgata aaagtcttt gtgcctttg tttctatta actaaattt ctaaagaca agaaaaatt tgagtagat agatcgaat agtgcttag ttaagctga aaaggtgct ttcctgaaa gagtgtttt tcgagctta gaggagagc tttggtgtg agtactcct tggaagtgg cataccttt cagcaaacg tgatcggca atagtaaat gctagcaca agccaataa aaatgtaac ctatgcatt agttattgt tccagcggc tgtcataaa acgtccgtg gtatatttt tagtgtagt atgaaacct gaaactgaa aacgacacc gaactagcg tccatgtag tacaagcaa cggccacgc gaggctcga aacacgcgc gctgcacgc aggtaaacc ttaccttta ccctaccta ccaggtatg gcgggtaca cagcccact gttcagtac cacggaacg cgagtacga gtttgcacg cgtgtctgt ccctccagc gaggcccgt cggaagcaa caagtgtgc atagatgtg aattcgaca gtcggcatc gtcgacgat cgctctgtt ggtgtcaaa tgcggcaaa agcgacggc cgtcggcga tgtagcctc gagtgatga cgggtccaa gtgatcgat tggtgctgc ggtgacact ccgggttaa gtgtcttta atgtaaaca ggaggattt tgtttgctt gttaaatag gcgagcaaa cagcagttt cttaatcaa accgtacca cgtcggaga gcaaatcgt agaatgtag caatcgaaa ctgacatat caaatgaag tggtgataa agtttcgtt tcaaagaca ggtgacttc gttacgtga tcaattaac gataaaaaa atggcctcc gacgaagac tgtagtact accttcacg taccctata atggacgac gcgacttcg tctcccaca actgccaac cactacagc aattgcttg tccccgtcc agctccccg tcgcccctt ttgccgggg cagtaccag ctcaccccc aggtacgag taccaagac caagacatg atccctgcg tcgcaggtg cttccccaa gtcctgcca aaaagccag gagatgtgg ggtgacatg agtcagggg atttacgcc gactaccgg tacgaaacg aaccgattt aacaacgat tacattcgg caaagttac gcggaaact atgcccgtg tttacgcaa actaggact aattttgtg ccgaaaata ggtgcacaa ggacaaaaa gtgcccaaa gaagcgagg attaggcgg ccgatgaac gcgttcatg gtgtgggcc aaagtggaa aggaagaaa ttggccgac gaaaatccg gatttgcat aatgccgat ttgagcaaa atgttgggc aagaagtgg cgctctttg acgccgcaa gacagacgg cctttcgtc gaagaagcc gaacgactg agagtgatc cacatgact gaacacccc aactacaaa tacagaccg cgacggcgg aagcacaac aagcagagg gccacttcc ggtgccgga ggcccccgg gtcggaacc tccttaccc agccccagt ctgcccaac atgtcgcca aggtacgcg ggctacatt cccaacgcg agtctttcg cccaacatt cagcagcag aacagctac ggaatggac ttccctagt cccggaact gccgacttc gcagcgcag gacaagaga tacagtccg gatactttc aagtaccac aaccagtac aactatgtg gggtaccag aactaccag aagagtccc tattcgatt caaacgcca gatgcgtct ccctcacac agtcccgag ccgaagaaa gggccgaat agtccgcag gattgtagc aaagacagt ccgcgggaa gatgcgtcc ctccctaca ccggaactg agtccaatg gagcacgaa cagttcgag gagaaaaga gtgtcgagt atacaacat acaagtaac ttgactaac ccggcgcag cagagttac aacacaaga gtacaaaac tacagacct aatagtacc aactacacc aacatccaa cccatcact tcggtacca atggcgaac gggatgtac gtgatgagt gctaataag agctcagtg gagcagggg catgtggtc actgggacg ttttaccca cccgtggct acgtcccag gaccaacaa ctgctgggt acgaaccaa aacaatctc aacactgtt gccaacaac acgaatttg cattactac aacgcgtcc atgcaacag tactacccc agtaccaac accgggtac gaccaagac caaaccaag ccagacacg ttcctaact tatcaaaat cctctcaaa tcggacgtc gtgaacgaa aatatcaac agttacaag tccgggggc ttggacgag tcgtaccaa ccgcagtac tccagcagc accctcgtg cagtcgtac atgggtcag gaggagcga agcgacgtc gacagcgat gtggacact cgagagttc gacaagtac ctcaagttt ggcagtacc gagtcgaat atgatcgac tcgaatcac aactaccac aggaacgac aatatcaat tcgaacgtg acttacaac ttccaagcc cagcatacg tctgtgatt ttgcccaac acgagtgtc aagcccgag ccgctgttg ggctacccc gaggtgtac gatatcggt cagaacggc gtcgtcgcg aagaacgat gacgatttt agtgagatt ctggcggac gtccggaag acgtgctac agtaactga cggtgcaat ttgagagtt tagttttat tcgaattgt tgttttatg gccagttgt tgtcgagtt gttgacgtc aaggagatg atttaaccc aaaagtttc ggattagag caaattcca ctgtacaca agtgtggtg tttaatttt ccatttcgg attaggttg ttaaattta cggttttgt aataatttt agtggtgcg caaacagat gctttaaat actccgacg agatctttg ttctctttg tgttaattg ttactttcg cagagtgcc ttatcatga tattatata caaagttgg tgtaaattt tcgcctaat tttagtgta aattattct atgcataga gatgattat atgttttgt acaatatta tttaagact ggctatttt tgtatcaag tcgagacaa taaataaac aatgacaat cagcgcgag acacgtcac ttaatatta ttatgaaaa cttttatct cagtgtttt accaatatt tgttcacac aaacttaaa taggatcta aggttaagc tgtttatat acctattta attatgtga aagagattt cttacaaaa aaccaaagt ttgtaaatt atgtatgat agtactcta gttatcggc tattcaatt gtaaatacc tagttatta cgtcatcat tgggaaata tatcattat ttaatta