Status
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Gene information
General information
TC033720
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009856,TC009854 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9157746..9208510
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033720
    iBeetle screen iB_01620

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033720 RA
      MASDEDCST TFTYPIMDD ATSSPTTAN HYSNCLSPS 
SSPSPLLPG QYQLTPRYE YQDQDMIPA SQVLPQVLP 
KSQEMWGDM SQGIYADYR YETNRFNND YIRQSYAET 
MPVFTQTRT NFVPKIGAQ GQKVPKEAR IRRPMNAFM 
VWAKVERKK LADENPDLH NADLSKMLG KKWRSLTPQ 
DRRPFVEEA ERLRVIHMT EHPNYKYRP RRRKHNKQR 
ATSGAGGPR VGTSLPSPS LPNMSPRYA GYIPNASLS 
PNIQQQNSY GMDFPSPGT ADFAAQDKR YSPDTFKYH 
NQYNYVGYQ NYQKSPYSI QTPDASPSH SPEPKKGPN 
SPQDCSKDS PREDASLPT PELSPMEHE QFEEKRVSS 
IQHTSNLTN PAQQSYNTR VQNYRPNST NYTNIQPIT 
SVPMANGMY VMSANKSSV EQGHVVTGT FYPPVATSQ 
DQQLLGTNQ NNLNTVANN TNLHYYNAS MQQYYPSTN 
TGYDQDQTK PDTFLTYQN PLKSDVVNE NINSYKSGG 
LDESYQPQY SSSTLVQSY MGQEERSDV DSDVDTREF 
DKYLKFGST ESNMIDSNH NYHRNDNIN SNVTYNFQA 
QHTSVILPN TSVKPEPLL GYPEVYDIG QNGVVAKND 
DDFSEILAD VRKTCYSN
    CDS sequence
    • >TC033720 RA
      atggcctcc gacgaagac tgtagtact accttcacg 
taccctata atggacgac gcgacttcg tctcccaca 
actgccaac cactacagc aattgcttg tccccgtcc 
agctccccg tcgcccctt ttgccgggg cagtaccag 
ctcaccccc aggtacgag taccaagac caagacatg 
atccctgcg tcgcaggtg cttccccaa gtcctgcca 
aaaagccag gagatgtgg ggtgacatg agtcagggg 
atttacgcc gactaccgg tacgaaacg aaccgattt 
aacaacgat tacattcgg caaagttac gcggaaact 
atgcccgtg tttacgcaa actaggact aattttgtg 
ccgaaaata ggtgcacaa ggacaaaaa gtgcccaaa 
gaagcgagg attaggcgg ccgatgaac gcgttcatg 
gtgtgggcc aaagtggaa aggaagaaa ttggccgac 
gaaaatccg gatttgcat aatgccgat ttgagcaaa 
atgttgggc aagaagtgg cgctctttg acgccgcaa 
gacagacgg cctttcgtc gaagaagcc gaacgactg 
agagtgatc cacatgact gaacacccc aactacaaa 
tacagaccg cgacggcgg aagcacaac aagcagagg 
gccacttcc ggtgccgga ggcccccgg gtcggaacc 
tccttaccc agccccagt ctgcccaac atgtcgcca 
aggtacgcg ggctacatt cccaacgcg agtctttcg 
cccaacatt cagcagcag aacagctac ggaatggac 
ttccctagt cccggaact gccgacttc gcagcgcag 
gacaagaga tacagtccg gatactttc aagtaccac 
aaccagtac aactatgtg gggtaccag aactaccag 
aagagtccc tattcgatt caaacgcca gatgcgtct 
ccctcacac agtcccgag ccgaagaaa gggccgaat 
agtccgcag gattgtagc aaagacagt ccgcgggaa 
gatgcgtcc ctccctaca ccggaactg agtccaatg 
gagcacgaa cagttcgag gagaaaaga gtgtcgagt 
atacaacat acaagtaac ttgactaac ccggcgcag 
cagagttac aacacaaga gtacaaaac tacagacct 
aatagtacc aactacacc aacatccaa cccatcact 
tcggtacca atggcgaac gggatgtac gtgatgagt 
gctaataag agctcagtg gagcagggg catgtggtc 
actgggacg ttttaccca cccgtggct acgtcccag 
gaccaacaa ctgctgggt acgaaccaa aacaatctc 
aacactgtt gccaacaac acgaatttg cattactac 
aacgcgtcc atgcaacag tactacccc agtaccaac 
accgggtac gaccaagac caaaccaag ccagacacg 
ttcctaact tatcaaaat cctctcaaa tcggacgtc 
gtgaacgaa aatatcaac agttacaag tccgggggc 
ttggacgag tcgtaccaa ccgcagtac tccagcagc 
accctcgtg cagtcgtac atgggtcag gaggagcga 
agcgacgtc gacagcgat gtggacact cgagagttc 
gacaagtac ctcaagttt ggcagtacc gagtcgaat 
atgatcgac tcgaatcac aactaccac aggaacgac 
aatatcaat tcgaacgtg acttacaac ttccaagcc 
cagcatacg tctgtgatt ttgcccaac acgagtgtc 
aagcccgag ccgctgttg ggctacccc gaggtgtac 
gatatcggt cagaacggc gtcgtcgcg aagaacgat 
gacgatttt agtgagatt ctggcggac gtccggaag 
acgtgctac agtaactga
    mRNA sequence
    • >TC033720 RA
      gccgtttcg gttcttcaa aaattgctt ccgtttaca 
tatatgata aaagtcttt gtgcctttg tttctatta 
actaaattt ctaaagaca agaaaaatt tgagtagat 
agatcgaat agtgcttag ttaagctga aaaggtgct 
ttcctgaaa gagtgtttt tcgagctta gaggagagc 
tttggtgtg agtactcct tggaagtgg cataccttt 
cagcaaacg tgatcggca atagtaaat gctagcaca 
agccaataa aaatgtaac ctatgcatt agttattgt 
tccagcggc tgtcataaa acgtccgtg gtatatttt 
tagtgtagt atgaaacct gaaactgaa aacgacacc 
gaactagcg tccatgtag tacaagcaa cggccacgc 
gaggctcga aacacgcgc gctgcacgc aggtaaacc 
ttaccttta ccctaccta ccaggtatg gcgggtaca 
cagcccact gttcagtac cacggaacg cgagtacga 
gtttgcacg cgtgtctgt ccctccagc gaggcccgt 
cggaagcaa caagtgtgc atagatgtg aattcgaca 
gtcggcatc gtcgacgat cgctctgtt ggtgtcaaa 
tgcggcaaa agcgacggc cgtcggcga tgtagcctc 
gagtgatga cgggtccaa gtgatcgat tggtgctgc 
ggtgacact ccgggttaa gtgtcttta atgtaaaca 
ggaggattt tgtttgctt gttaaatag gcgagcaaa 
cagcagttt cttaatcaa accgtacca cgtcggaga 
gcaaatcgt agaatgtag caatcgaaa ctgacatat 
caaatgaag tggtgataa agtttcgtt tcaaagaca 
ggtgacttc gttacgtga tcaattaac gataaaaaa 
atggcctcc gacgaagac tgtagtact accttcacg 
taccctata atggacgac gcgacttcg tctcccaca 
actgccaac cactacagc aattgcttg tccccgtcc 
agctccccg tcgcccctt ttgccgggg cagtaccag 
ctcaccccc aggtacgag taccaagac caagacatg 
atccctgcg tcgcaggtg cttccccaa gtcctgcca 
aaaagccag gagatgtgg ggtgacatg agtcagggg 
atttacgcc gactaccgg tacgaaacg aaccgattt 
aacaacgat tacattcgg caaagttac gcggaaact 
atgcccgtg tttacgcaa actaggact aattttgtg 
ccgaaaata ggtgcacaa ggacaaaaa gtgcccaaa 
gaagcgagg attaggcgg ccgatgaac gcgttcatg 
gtgtgggcc aaagtggaa aggaagaaa ttggccgac 
gaaaatccg gatttgcat aatgccgat ttgagcaaa 
atgttgggc aagaagtgg cgctctttg acgccgcaa 
gacagacgg cctttcgtc gaagaagcc gaacgactg 
agagtgatc cacatgact gaacacccc aactacaaa 
tacagaccg cgacggcgg aagcacaac aagcagagg 
gccacttcc ggtgccgga ggcccccgg gtcggaacc 
tccttaccc agccccagt ctgcccaac atgtcgcca 
aggtacgcg ggctacatt cccaacgcg agtctttcg 
cccaacatt cagcagcag aacagctac ggaatggac 
ttccctagt cccggaact gccgacttc gcagcgcag 
gacaagaga tacagtccg gatactttc aagtaccac 
aaccagtac aactatgtg gggtaccag aactaccag 
aagagtccc tattcgatt caaacgcca gatgcgtct 
ccctcacac agtcccgag ccgaagaaa gggccgaat 
agtccgcag gattgtagc aaagacagt ccgcgggaa 
gatgcgtcc ctccctaca ccggaactg agtccaatg 
gagcacgaa cagttcgag gagaaaaga gtgtcgagt 
atacaacat acaagtaac ttgactaac ccggcgcag 
cagagttac aacacaaga gtacaaaac tacagacct 
aatagtacc aactacacc aacatccaa cccatcact 
tcggtacca atggcgaac gggatgtac gtgatgagt 
gctaataag agctcagtg gagcagggg catgtggtc 
actgggacg ttttaccca cccgtggct acgtcccag 
gaccaacaa ctgctgggt acgaaccaa aacaatctc 
aacactgtt gccaacaac acgaatttg cattactac 
aacgcgtcc atgcaacag tactacccc agtaccaac 
accgggtac gaccaagac caaaccaag ccagacacg 
ttcctaact tatcaaaat cctctcaaa tcggacgtc 
gtgaacgaa aatatcaac agttacaag tccgggggc 
ttggacgag tcgtaccaa ccgcagtac tccagcagc 
accctcgtg cagtcgtac atgggtcag gaggagcga 
agcgacgtc gacagcgat gtggacact cgagagttc 
gacaagtac ctcaagttt ggcagtacc gagtcgaat 
atgatcgac tcgaatcac aactaccac aggaacgac 
aatatcaat tcgaacgtg acttacaac ttccaagcc 
cagcatacg tctgtgatt ttgcccaac acgagtgtc 
aagcccgag ccgctgttg ggctacccc gaggtgtac 
gatatcggt cagaacggc gtcgtcgcg aagaacgat 
gacgatttt agtgagatt ctggcggac gtccggaag 
acgtgctac agtaactga cggtgcaat ttgagagtt 
tagttttat tcgaattgt tgttttatg gccagttgt 
tgtcgagtt gttgacgtc aaggagatg atttaaccc 
aaaagtttc ggattagag caaattcca ctgtacaca 
agtgtggtg tttaatttt ccatttcgg attaggttg 
ttaaattta cggttttgt aataatttt agtggtgcg 
caaacagat gctttaaat actccgacg agatctttg 
ttctctttg tgttaattg ttactttcg cagagtgcc 
ttatcatga tattatata caaagttgg tgtaaattt 
tcgcctaat tttagtgta aattattct atgcataga 
gatgattat atgttttgt acaatatta tttaagact 
ggctatttt tgtatcaag tcgagacaa taaataaac 
aatgacaat cagcgcgag acacgtcac ttaatatta 
ttatgaaaa cttttatct cagtgtttt accaatatt 
tgttcacac aaacttaaa taggatcta aggttaagc 
tgtttatat acctattta attatgtga aagagattt 
cttacaaaa aaccaaagt ttgtaaatt atgtatgat 
agtactcta gttatcggc tattcaatt gtaaatacc 
tagttatta cgtcatcat tgggaaata tatcattat 
ttaatta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY