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Gene information
General information
TC009846 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8986427..8993713
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009846
    iBeetle screen iB_01618

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009846 RA
      MGVPAFFRW LSRKYPSVI VHCIEQKAV DVNGVRVPT 
      NSAEPNPNG VEFDNLYLD MNGIIHPCT HPEDRPAPR 
      NEDEMMVAI FECIDRIFN IVRPRKLLY MAIDGVAPR 
      AKMNQQRSR RFRAAKETV EKINEIKRI RAELLLRGA 
      KLPDEKPKE EHFDSNCIT PGTPFMARL SNCLHYYIH 
      ERLNNDPGW QGIKVILSD ANVPGEGEH KIMDFIRRQ 
      RAQPDHDPN TQHCLCGAD ADLIMLGLA THEPNFTII 
      REEFKPNKP RPCDICGQL GHEMKDCIG GAASAESDT 
      QKVFAETDY IFVRLNVLK EYLERELQM PNLPFKYEF 
      ERVLDDWVF MCFFVGNDF LPHLPSLEI REGAIDRLI 
      RLYKDCVYK TGGWLTNSG DVNLERVQL IMSELGKAE 
      DEIFKTRQQ KELQFKARE KAKRRRSEG YNQYKPNWD 
      LVKNTQFAP TALGQAKPV VNARHEAFE IRKAGMHTQ 
      KAGLESMLT PVDSQSSNR GQKRHHEDD DESDDDQAH 
      DEVRLWEDG FKDRYYESK FDVNSDNLD FRNNVALHY 
      VRGLCWVLR YYYQGCASW KWYYPYHYA PFASDFVHI 
      AGLSTEFEE DSDPFRPLE QLMGVFPAA SSKHVPQPW 
      AKLMSDPES PIIDFYPED FKIDLNGKK FAWQGVALL 
      PFVNEDRLF KALAPYYKE LSPEEIRRN VRGDDRLYV 
      GAKNAGYNQ IKQLYVQKI SVKMEVHVS IEGMQGTVL 
      LSDECVDYG GYLDSPIRG LDDIMNNMV SCVKYRDPK 
      YASGFVFPA KKLKGAIDP PRVLKPTDL DPVASRNWR 
      PIIGMVPST TRATLGEAG HRMVGHHFP KSGPGPYSH 
      VPPPSNMDR RNNYHQNRG SNYGHSQGG YSSYNQGHN 
      SRGYQSGYD TGYNQRYNR NNYQNRNYN SDRNDPSRR 
      PPGPYRDGY QTSSSNPNA NRYSYRR
    CDS sequence
    • >TC009846 RA
      atgggagtc ccggcattt ttccgttgg ctgagccgc 
      aaataccct tcagtgatc gtccactgc atcgaacaa 
      aaagcagtt gatgtaaat ggggtccgg gttccaacc 
      aattccgcc gagcccaac cccaacggg gtggagttt 
      gacaatttg tacctggac atgaacgga attatccac 
      ccgtgcacg catccagag gaccgacca gcgccgcgc 
      aatgaagac gagatgatg gtcgcgatt ttcgaatgc 
      atcgatcgc attttcaac atcgtccgt cccaggaaa 
      ctcctgtac atggcgatt gacggagtt gccccacgc 
      gcgaaaatg aaccagcag agatcaagg cgtttccgc 
      gccgctaaa gaaaccgtg gagaaaatt aacgaaatc 
      aagcgaatt cgggccgag ctgttgctg cgaggggcg 
      aagttgccg gacgagaaa ccgaaggag gagcacttt 
      gattccaac tgcatcacg ccggggacc ccgttcatg 
      gcgcggctt tctaactgc ctgcattat tacatccac 
      gagaggctg aataatgac ccagggtgg caggggatt 
      aaagttatc ctctcggat gcgaacgtg ccgggtgaa 
      ggcgaacac aaaatcatg gattttatt aggcgccag 
      agggcgcaa cctgaccac gatccaaac actcaacac 
      tgcctttgt ggtgccgac gctgactta atcatgttg 
      ggtttggcc acgcatgag cccaatttt actataatt 
      agggaagag ttcaagcct aacaagccc agaccttgt 
      gatatttgt ggtcagttg ggtcacgaa atgaaagat 
      tgtataggt ggcgctgct agtgctgag agcgacacg 
      cagaaagtg tttgcagaa acggattat atttttgtt 
      cgtttgaac gttttaaag gagtattta gaacgcgaa 
      ttgcaaatg ccgaattta cccttcaaa tacgagttt 
      gagcgggtt ttagacgat tgggttttt atgtgcttt 
      tttgttggt aacgatttt ttaccacat ttgccaagc 
      ttggaaatt cgggaaggg gcaattgac cgactaatc 
      agactatat aaagattgc gtctataaa actgggggc 
      tggttgaca aacagcggt gatgtcaac ttggagcgc 
      gttcagttg attatgagc gaattgggc aaagctgag 
      gatgaaata tttaaaaca cgacaacag aaagaatta 
      cagtttaag gcgagagaa aaggcgaaa agacggagg 
      agcgaaggc tacaaccag tataaaccc aactgggac 
      ttggtcaag aacacgcag tttgctcct accgcttta 
      ggccaggca aagccggta gtaaacgcc cgtcatgag 
      gcttttgaa atacgaaag gccggaatg cacactcaa 
      aaagcgggg ttagagtcg atgttaaca cccgtggat 
      agtcagtca agtaaccgg ggccagaag cgccaccat 
      gaggacgac gatgaaagt gatgacgac caggctcat 
      gatgaagtg agattatgg gaagatggg ttcaaggat 
      cgttactat gaatcaaaa ttcgacgtg aattctgat 
      aatctagat tttcgaaat aacgtagcg ctgcattac 
      gtgaggggt ttgtgttgg gttttgcgg tactattac 
      caagggtgt gcctcgtgg aagtggtac tatccctac 
      cattatgcc ccatttgct tcagatttt gttcacatc 
      gcaggcttg agtactgaa tttgaggaa gactctgat 
      cctttccga cctctagaa caattaatg ggtgttttc 
      ccggctgct agtagtaaa catgttcct caaccatgg 
      gcaaagtta atgtcagac cctgaatca ccaattatt 
      gatttttat cccgaagat tttaaaatt gacttaaac 
      gggaagaaa tttgcctgg caaggagtt gcgttattg 
      ccttttgtt aatgaggat aggttattt aaggcctta 
      gctccatat tataaagag ttatcacct gaagaaatt 
      cggagaaat gtgcgagga gatgatcgg ttgtatgtt 
      ggggccaaa aacgccggt tataaccaa ataaagcaa 
      ctgtatgta caaaaaatc agtgtgaaa atggaagtg 
      cacgtttcc attgaggga atgcagggg actgtgttg 
      ttgtccgat gaatgcgtc gattatgga ggttatttg 
      gattctccg attcgcggt ttggacgac atcatgaac 
      aacatggtg tcttgtgtg aaataccgc gatccaaaa 
      tacgcttcc ggcttcgta tttccagcc aagaaacta 
      aaaggcgcg atcgatccg cctcgagtt ttaaaacca 
      acagattta gatcctgta gcaagcaga aactggcga 
      cctatcatt ggaatggtg ccatcaaca acacgagcc 
      actttaggc gaggccggc cacaggatg gtgggtcat 
      cattttccc aagtcgggt ccaggtccg tactcgcac 
      gttcctccg ccgtctaat atggacaga agaaataac 
      tatcaccaa aatcgcggc agtaattac gggcacagt 
      caaggaggg tactcgagt tacaaccag gggcacaac 
      tcgaggggg taccagagt gggtatgac acagggtac 
      aatcaaagg tacaaccgg aacaactac caaaatcgg 
      aactacaac tccgacagg aacgatccc agcagaagg 
      cctccgggt ccttacagg gacggatat cagacctcc 
      agctcgaac cctaacgca aatagatac agctacagg 
      aggtag
    mRNA sequence
    • >TC009846 RA
      ttacagtag tgactcaag atggctgtc atgcattta 
      acttttgaa taaattcaa aattcttaa tttcctggc 
      gactatcta cgttcttat tagcattcc gaccttttg 
      caatgggag tcccggcat ttttccgtt ggctgagcc 
      gcaaatacc cttcagtga tcgtccact gcatcgaac 
      aaaaagcag ttgatgtaa atggggtcc gggttccaa 
      ccaattccg ccgagccca accccaacg gggtggagt 
      ttgacaatt tgtacctgg acatgaacg gaattatcc 
      acccgtgca cgcatccag aggaccgac cagcgccgc 
      gcaatgaag acgagatga tggtcgcga ttttcgaat 
      gcatcgatc gcattttca acatcgtcc gtcccagga 
      aactcctgt acatggcga ttgacggag ttgccccac 
      gcgcgaaaa tgaaccagc agagatcaa ggcgtttcc 
      gcgccgcta aagaaaccg tggagaaaa ttaacgaaa 
      tcaagcgaa ttcgggccg agctgttgc tgcgagggg 
      cgaagttgc cggacgaga aaccgaagg aggagcact 
      ttgattcca actgcatca cgccgggga ccccgttca 
      tggcgcggc tttctaact gcctgcatt attacatcc 
      acgagaggc tgaataatg acccagggt ggcagggga 
      ttaaagtta tcctctcgg atgcgaacg tgccgggtg 
      aaggcgaac acaaaatca tggatttta ttaggcgcc 
      agagggcgc aacctgacc acgatccaa acactcaac 
      actgccttt gtggtgccg acgctgact taatcatgt 
      tgggtttgg ccacgcatg agcccaatt ttactataa 
      ttagggaag agttcaagc ctaacaagc ccagacctt 
      gtgatattt gtggtcagt tgggtcacg aaatgaaag 
      attgtatag gtggcgctg ctagtgctg agagcgaca 
      cgcagaaag tgtttgcag aaacggatt atatttttg 
      ttcgtttga acgttttaa aggagtatt tagaacgcg 
      aattgcaaa tgccgaatt tacccttca aatacgagt 
      ttgagcggg ttttagacg attgggttt ttatgtgct 
      tttttgttg gtaacgatt ttttaccac atttgccaa 
      gcttggaaa ttcgggaag gggcaattg accgactaa 
      tcagactat ataaagatt gcgtctata aaactgggg 
      gctggttga caaacagcg gtgatgtca acttggagc 
      gcgttcagt tgattatga gcgaattgg gcaaagctg 
      aggatgaaa tatttaaaa cacgacaac agaaagaat 
      tacagttta aggcgagag aaaaggcga aaagacgga 
      ggagcgaag gctacaacc agtataaac ccaactggg 
      acttggtca agaacacgc agtttgctc ctaccgctt 
      taggccagg caaagccgg tagtaaacg cccgtcatg 
      aggcttttg aaatacgaa aggccggaa tgcacactc 
      aaaaagcgg ggttagagt cgatgttaa cacccgtgg 
      atagtcagt caagtaacc ggggccaga agcgccacc 
      atgaggacg acgatgaaa gtgatgacg accaggctc 
      atgatgaag tgagattat gggaagatg ggttcaagg 
      atcgttact atgaatcaa aattcgacg tgaattctg 
      ataatctag attttcgaa ataacgtag cgctgcatt 
      acgtgaggg gtttgtgtt gggttttgc ggtactatt 
      accaagggt gtgcctcgt ggaagtggt actatccct 
      accattatg ccccatttg cttcagatt ttgttcaca 
      tcgcaggct tgagtactg aatttgagg aagactctg 
      atcctttcc gacctctag aacaattaa tgggtgttt 
      tcccggctg ctagtagta aacatgttc ctcaaccat 
      gggcaaagt taatgtcag accctgaat caccaatta 
      ttgattttt atcccgaag attttaaaa ttgacttaa 
      acgggaaga aatttgcct ggcaaggag ttgcgttat 
      tgccttttg ttaatgagg ataggttat ttaaggcct 
      tagctccat attataaag agttatcac ctgaagaaa 
      ttcggagaa atgtgcgag gagatgatc ggttgtatg 
      ttggggcca aaaacgccg gttataacc aaataaagc 
      aactgtatg tacaaaaaa tcagtgtga aaatggaag 
      tgcacgttt ccattgagg gaatgcagg ggactgtgt 
      tgttgtccg atgaatgcg tcgattatg gaggttatt 
      tggattctc cgattcgcg gtttggacg acatcatga 
      acaacatgg tgtcttgtg tgaaatacc gcgatccaa 
      aatacgctt ccggcttcg tatttccag ccaagaaac 
      taaaaggcg cgatcgatc cgcctcgag ttttaaaac 
      caacagatt tagatcctg tagcaagca gaaactggc 
      gacctatca ttggaatgg tgccatcaa caacacgag 
      ccactttag gcgaggccg gccacagga tggtgggtc 
      atcattttc ccaagtcgg gtccaggtc cgtactcgc 
      acgttcctc cgccgtcta atatggaca gaagaaata 
      actatcacc aaaatcgcg gcagtaatt acgggcaca 
      gtcaaggag ggtactcga gttacaacc aggggcaca 
      actcgaggg ggtaccaga gtgggtatg acacagggt 
      acaatcaaa ggtacaacc ggaacaact accaaaatc 
      ggaactaca actccgaca ggaacgatc ccagcagaa 
      ggcctccgg gtccttaca gggacggat atcagacct 
      ccagctcga accctaacg caaatagat acagctaca 
      ggaggtaga aattttcat ttctacata cttctaatt 
      ttatccgac tttgtaact tttttaatc acttaaaaa 
      aataactat cccttcact atttatttg agttaaaat 
      gtatagtgt aggttcatt gagagaaca gtattctct 
      tgttttgta tgattatgg ttttgtacc tttctagca 
      aaggcttaa ggaaaagaa ttgttgtag tttaaaaag 
      tatggaata aacaatatg attttag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY