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Gene information
General information
TC009844 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 2306270..2312154
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009844
    iBeetle screen iB_01617
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    labrum bent - Penetrance (percentage of animals/eggs on slide): less than 30%

    metathorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen partially constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen complete not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009844 RA
      MATLWYMFV CFCWWSLVN SDVLQENLS NKSESHSSE 
      ISAVLNHLN SSPFKVYNY NKATLLNAQ TKADIIELI 
      LDIDVDCIN KYPNVPCGE KVVTCHAFV RNTPEEGSK 
      VLTDVNCKP KPEIIVDEP VYDSPTNPT EVNTNEPTP 
      IVLSNEAVS SEVGEHFVA VRKDSVPCL GCPFDLNTN 
      AEGVDELID VALEHIQTE RAKKHALVK VLRLQQQVV 
      TGVKYILTA EFAPTLCEK SANLDASSC PRDTNAETT 
      ICEITYLHK PWISKAKHV IKNNCTVSQ EYVRPDIRK 
      SDISNEIDV SGKRTKLID SPNFDKMAE NGAIDEMTP 
      SRLADLESQ IIVDVEPEI GAPSTAESH PQVESKLEF 
      DLFQTPESL TVSEDPAFT AGVTVEEPA PTEEHVSRL 
      KRDESSEET KKDSSSESV ETAANQTKA TKTSSSEEN 
      SSEEVLRPK RAIRQLEKI SPDEKGLVK NLADFAANA 
      LDSIDDDNN KRIILQILG AKKMVGDDG VYYHIIMRM 
      GVSRCLEDS PINPYENCK DKLFENYTK ICKVQVYVN 
      DDFGSKKVV KSQCQNIKR DKNDNNRTN YSRYKRDGL 
      GAPNRIDKN SEKIRQFVK EGINGFNAN YNSKNNKVK 
      PVEVVSATT QVVAGTLYK ITTKISESD CSKNDNKDL 
      DDCNILEGA SPKTCELEV WEKLWENFR QFTIKCEGD 
      EQSIKFQTS PKNRAKRQA PGGESPVTK DNEYVVKYL 
      EAALNQLDS ESPHENKFK VHEFISATS QTVSGHIYR 
      IKTKVVLSD CKKTASTER GQCGTLKDA KPKTCKFEV 
      FEQTWVPNS RRIKTDCDK ERRKRKTLD GGITEVDKD 
      DDEVKTFVR EGLLNLNTH LTTSNKVKP VEVVSASVQ 
      VVAGSLHRI KVKISESDC SRNDQKDFE QCNVLEGAS 
      PKLCEMEVW DKPWEDFRR YTIKCEGEN KKHQFTLNP 
      NKRTKRQVP RGESPVAKD NEYVIKYLE AALNQLDAE 
      SEHENKFKV HEFISATSQ IVSGHIYRI NAKVILSDC 
      KKTVSTERG QCGTLKDAK PKTCKFEVF EQLWVPNSR 
      QIKTDCHKE RRKRQTLPG GIKEVDKNN DEVKTFVHE 
      GLLNLNTHL TTSNKVKPV EVVSASVQV VAGSLHRIK 
      VKISESDCS RNDQKDFEQ CNVLEGASP KLCEMEVWD 
      KPWEDFRRY TIKCEGENK KHQFTLNPN KRTKRQVRG 
      GESPVAKDN EYVIKYLEA ALNQLDAES EHENKFKVH 
      EFISATSQI VSGHIYRIK AKVILSDCK KSISTERGQ 
      CGTLKDAKP KTCKFEVFE QLWVPNSRQ IKTECDGKR 
      VKRQRSEKP HSVRSKRQS IPGGATEID KKSDKVKQY 
      VRESLTHLN TQLTSSNKV KPVEVLSAT SQVVAGTIH 
      RIKVKISES DCSKDDEKD FDECNIREG ASPKICEVK 
      VWDKPWQNF RQYNITCEG EDHHYTFET KPGKTKRSA 
      ELDNLLGCD DREYHLSLF TDFLKKYNK KYHKKEYKY 
      RFNVFVQNL MQIRVLNTF EQGTATYGI TRFADMTQK 
      EFSRSLGLR TDLRNENET PFAQAKIPN IELPKEFDW 
      RKKNVVTEV KNQEQCGSC WAFSVTGNV EGQYALRHG 
      KLLEFSEQE LVDCDTDDQ GCNGGLMDT AYRSIEKIG 
      GLETEQDYP YDAEDEKCH FNRTLARVQ VTGALNISH 
      NETDMAKWL VANGPISIA INANAMQFY MGGVSHPFK 
      FLCSPKNLD HGVLIVGYG VHNYPLFKK SLPYWIVKN 
      SWGTGWGEQ GYYRVYRGD GTCGLNQTP SSAIVA
    CDS sequence
    • >TC009844 RA
      atggcgacc ttgtggtac atgtttgtt tgtttctgt 
      tggtggtct ttagtaaat agtgatgtt ttacaagaa 
      aatttatcg aacaaatca gaatctcat tcttccgaa 
      atctcggca gtgttaaat cacctgaat tcgtctcct 
      ttcaaggtg tacaactac aacaaagca accctccta 
      aatgcgcag acaaaggct gatatcatc gaattaatt 
      ctcgacata gatgtggat tgcatcaac aagtacccc 
      aatgtgccc tgcggcgaa aaagtcgtc acttgtcac 
      gccttcgtc aggaatacg cctgaagaa ggcagcaag 
      gtgctcact gatgtcaat tgcaaacct aaacctgag 
      attatcgtt gatgagcca gtgtatgac tcaccaaca 
      aacccaacc gaagtgaac acaaatgaa ccaacacct 
      attgtgtta agcaacgaa gctgtttcg tctgaagtg 
      ggcgagcat ttcgtcgcg gtcaggaaa gactcagtt 
      ccttgttta ggctgcccc tttgacctc aacacaaat 
      gcggaaggc gtggatgaa ctaattgac gtagcttta 
      gagcacatt cagacggaa cgtgccaaa aaacatgcg 
      ctggtcaaa gtactcaga ttacaacaa caagttgta 
      actggagtt aaatatatc ctgacggcg gaattcgcg 
      cccacactt tgcgaaaaa agtgcaaac cttgacgcg 
      tcttcatgc ccaagagac acaaatgca gaaacaaca 
      atttgcgag atcacctac ttgcataaa ccttggata 
      agtaaagcc aaacacgtg atcaaaaat aattgtacc 
      gtttcgcaa gaatacgtc aggcccgat attaggaag 
      tctgatata agtaatgaa attgatgtt agcggtaaa 
      cgtactaaa ctaattgac tctcccaat tttgataaa 
      atggcagaa aacggggct attgacgaa atgacaccc 
      tcgcggtta gcggatttg gagtcacaa ataatcgtt 
      gatgtggag cctgaaatc ggagcgccc tccactgcg 
      gagtcacac cctcaggta gagagcaaa cttgagttt 
      gatttattt caaacacct gaaagtctg acggtgtct 
      gaagatccc gcatttacg gcgggtgtt accgtggag 
      gagcctgcg cccactgaa gaacatgtt tcgaggttg 
      aaacgtgac gaaagttca gaagaaact aagaaagat 
      tcttcaagt gaatcagtc gaaacggca gcaaaccaa 
      accaaagct acgaaaacc agcagctct gaggaaaat 
      tcaagtgag gaggtttta agaccaaag agagcaatc 
      cgtcagttg gagaaaatc tcgcctgat gagaaaggc 
      ttggtgaag aatttggcc gactttgcc gccaatgcg 
      ctagacagc attgacgat gataacaac aaacgaata 
      attttgcaa attttgggg gcgaaaaaa atggtcggg 
      gacgatggg gtttattat cacattatc atgcggatg 
      ggagtctcg cgttgcttg gaggatagc ccaatcaat 
      ccgtacgaa aattgcaag gacaagctt ttcgaaaat 
      tatacgaaa atttgcaaa gtccaggtt tacgtgaac 
      gatgatttt ggtagcaaa aaagtggta aagtcccaa 
      tgtcagaac ataaaacgc gataaaaat gacaacaat 
      cgcacgaat tacagtcgc tataagcga gatggctta 
      ggggccccg aatcgcatt gataaaaat agcgagaaa 
      attcgacaa tttgtcaaa gaagggatt aacggtttt 
      aatgccaat tacaatagt aaaaataac aaagtgaag 
      ccagttgaa gtggtttca gcaacaacc caagtcgtt 
      gcagggaca ctttacaaa atcacgacg aaaatttcc 
      gaatctgat tgttctaaa aacgataat aaagattta 
      gatgattgt aatattctt gaaggggct tcgccaaaa 
      acatgcgag ttggaggtg tgggagaaa ctgtgggag 
      aatttcaga caattcaca ataaagtgt gaaggggac 
      gaacaaagt attaaattt caaacaagc cctaaaaat 
      agagctaag cgtcaagct cccggcgga gagtcaccg 
      gtgaccaaa gacaacgag tacgttgtt aagtatttg 
      gaagcggct ttaaaccaa ctcgattct gaatctcca 
      cacgagaat aagtttaaa gtccacgag tttatttct 
      gccacctcg caaaccgtc tcgggtcac atttatcga 
      atcaaaacg aaagtagta ctttccgat tgtaaaaaa 
      actgcttcg accgagaga ggtcaatgt gggaccctc 
      aaagatgcg aaacccaaa acttgcaaa tttgaggtt 
      tttgaacaa acttgggtt cctaacagc cgtcgaatt 
      aaaactgac tgtgacaag gagaggaga aaaaggaaa 
      acgttggac ggtggaata acggaagtc gataaagac 
      gacgatgaa gtgaaaaca tttgttcgt gaaggttta 
      ctcaatttg aatactcat ttgactaca agtaataaa 
      gtcaagcct gtagaagtg gtttctgct tcagttcaa 
      gttgtggca gggtctttg cataggatt aaagtgaaa 
      atatcagag tcggattgt tccaggaac gatcagaaa 
      gacttcgaa cagtgtaat gtacttgag ggggcttcc 
      ccgaaattg tgtgagatg gaggtttgg gataaacca 
      tgggaggac ttcaggagg tacactata aagtgtgaa 
      ggcgagaat aagaaacac cagtttacg ttaaatcca 
      aacaagcga actaagcgt caagttccc aggggagaa 
      tcaccagtg gcgaaagac aatgaatac gtcattaaa 
      tatctggag gctgcttta aatcaactt gatgctgaa 
      tctgaacac gaaaataag tttaaagtc catgagttt 
      atttcagca acatcgcag attgtctcc ggtcacatt 
      tatcgcatc aatgcgaaa gtgatactt tccgattgt 
      aaaaaaaca gtttcgacc gagagaggc cagtgtggt 
      actctcaaa gacgccaaa cccaaaact tgcaaattt 
      gaagtgttt gaacaactt tgggtacct aatagtcgt 
      caaattaaa accgattgc cacaaagag agaagaaaa 
      aggcaaaca ttgccaggt ggaataaag gaagtcgat 
      aaaaacaac gatgaagtg aaaacattt gtacatgaa 
      ggtttactc aatttgaat actcatttg actacaagt 
      aacaaagtc aagcctgta gaagtggtt tctgcttca 
      gttcaagtt gtggcaggg tctttgcat aggattaaa 
      gtgaaaata tcagagtcg gattgttcc aggaacgat 
      cagaaagac ttcgaacag tgtaatgta ctcgaagga 
      gcttccccg aagttgtgt gagatggag gtttgggat 
      aaaccatgg gaggacttc aggaggtac actataaag 
      tgtgaaggc gaaaataag aaacatcag tttacgtta 
      aatccaaac aagcgaact aagcgtcaa gttcggggg 
      ggagaatca ccagtggcg aaagacaac gaatacgtc 
      attaaatat ctggaggct gctttaaat caacttgat 
      gctgaatct gaacacgaa aataagttt aaagtccat 
      gagtttatt tcagcaaca tcgcagatt gtctccggc 
      cacatttat cgcatcaaa gcgaaagta atactttcc 
      gattgtaaa aaatcaatt tcgactgaa agaggccag 
      tgtggtact ctcaaagac gccaaaccc aaaacttgc 
      aaatttgaa gtgtttgaa cagctttgg gtacctaat 
      agtcgtcaa attaaaacc gagtgtgac ggtaaaaga 
      gtaaaaagg caaaggagc gaaaaacca cactccgta 
      aggagtaag aggcagtca atacctggt ggagcaacc 
      gaaattgat aaaaaaagc gataaagtc aagcaatac 
      gtccgtgaa agcttaact cacttaaac actcaactg 
      acttccagc aataaggtc aagcctgtg gaagtactt 
      tctgccacg agtcaagtc gttgcagga acaatccat 
      agaatcaaa gtaaaaata tccgaatct gattgttct 
      aaagacgat gaaaaagac ttcgatgag tgtaacatc 
      cgcgaaggt gcttccccg aaaatatgc gaagtaaag 
      gtttgggat aagccgtgg cagaatttc cgacagtac 
      aatataacc tgcgagggt gaggatcac cactacaca 
      ttcgaaaca aaacccgga aaaacaaaa cgcagtgcc 
      gagttggac aatttattg ggttgcgac gaccgagaa 
      taccattta tccctcttc actgacttc ctcaaaaaa 
      tacaacaag aaatatcac aagaaagaa tacaaatac 
      agatttaat gtatttgta caaaacttg atgcaaatc 
      agggtattg aataccttt gaacagggg acggcaacg 
      tatggaatt acgaggttt gcggatatg actcaaaaa 
      gagttttcg cggagtttg ggtttgcgt acggatttg 
      aggaacgag aatgagact ccgttcgca caagctaaa 
      attcccaat attgaactt ccgaaggag tttgattgg 
      cgaaagaaa aatgtcgtg acggaggtc aagaatcag 
      gaacagtgt ggaagttgc tgggcgttt agcgtcacg 
      ggaaatgtg gaagggcag tatgctttg agacacggg 
      aagttgttg gagttttcc gaacaggag ttggtggac 
      tgtgatacg gatgaccaa gggtgtaat ggagggttg 
      atggacacc gcttacaga tcgattgag aaaatcggt 
      ggcttggag acagagcag gactacccc tacgacgcc 
      gaggacgaa aaatgccat ttcaatcga accttagcc 
      cgcgtccag gtcactggg gccctcaac atcagccac 
      aacgagacc gatatggcc aagtggttg gtggccaat 
      ggccccatt tccatcgcc atcaatgcc aacgctatg 
      cagttttac atgggcggg gtgtcccat ccattcaag 
      tttttgtgt agtccgaaa aaccttgac cacggggtt 
      ttaatagtg ggttacggt gtacacaat tatccactc 
      ttcaagaag agtcttccg tattggatc gtcaaaaac 
      tcgtggggg acaggatgg ggcgaacaa ggttactat 
      agagtttac aggggggac ggtacgtgt ggtttgaac 
      cagactccc tccagtgcc atagtagct taa
    mRNA sequence
    • >TC009844 RA
      ctgattttg acacttttc agctttgac atcaatcag 
      aaacaaaca tggcgacct tgtggtaca tgtttgttt 
      gtttctgtt ggtggtctt tagtaaata gtgatgttt 
      tacaagaaa atttatcga acaaatcag aatctcatt 
      cttccgaaa tctcggcag tgttaaatc acctgaatt 
      cgtctcctt tcaaggtgt acaactaca acaaagcaa 
      ccctcctaa atgcgcaga caaaggctg atatcatcg 
      aattaattc tcgacatag atgtggatt gcatcaaca 
      agtacccca atgtgccct gcggcgaaa aagtcgtca 
      cttgtcacg ccttcgtca ggaatacgc ctgaagaag 
      gcagcaagg tgctcactg atgtcaatt gcaaaccta 
      aacctgaga ttatcgttg atgagccag tgtatgact 
      caccaacaa acccaaccg aagtgaaca caaatgaac 
      caacaccta ttgtgttaa gcaacgaag ctgtttcgt 
      ctgaagtgg gcgagcatt tcgtcgcgg tcaggaaag 
      actcagttc cttgtttag gctgcccct ttgacctca 
      acacaaatg cggaaggcg tggatgaac taattgacg 
      tagctttag agcacattc agacggaac gtgccaaaa 
      aacatgcgc tggtcaaag tactcagat tacaacaac 
      aagttgtaa ctggagtta aatatatcc tgacggcgg 
      aattcgcgc ccacacttt gcgaaaaaa gtgcaaacc 
      ttgacgcgt cttcatgcc caagagaca caaatgcag 
      aaacaacaa tttgcgaga tcacctact tgcataaac 
      cttggataa gtaaagcca aacacgtga tcaaaaata 
      attgtaccg tttcgcaag aatacgtca ggcccgata 
      ttaggaagt ctgatataa gtaatgaaa ttgatgtta 
      gcggtaaac gtactaaac taattgact ctcccaatt 
      ttgataaaa tggcagaaa acggggcta ttgacgaaa 
      tgacaccct cgcggttag cggatttgg agtcacaaa 
      taatcgttg atgtggagc ctgaaatcg gagcgccct 
      ccactgcgg agtcacacc ctcaggtag agagcaaac 
      ttgagtttg atttatttc aaacacctg aaagtctga 
      cggtgtctg aagatcccg catttacgg cgggtgtta 
      ccgtggagg agcctgcgc ccactgaag aacatgttt 
      cgaggttga aacgtgacg aaagttcag aagaaacta 
      agaaagatt cttcaagtg aatcagtcg aaacggcag 
      caaaccaaa ccaaagcta cgaaaacca gcagctctg 
      aggaaaatt caagtgagg aggttttaa gaccaaaga 
      gagcaatcc gtcagttgg agaaaatct cgcctgatg 
      agaaaggct tggtgaaga atttggccg actttgccg 
      ccaatgcgc tagacagca ttgacgatg ataacaaca 
      aacgaataa ttttgcaaa ttttggggg cgaaaaaaa 
      tggtcgggg acgatgggg tttattatc acattatca 
      tgcggatgg gagtctcgc gttgcttgg aggatagcc 
      caatcaatc cgtacgaaa attgcaagg acaagcttt 
      tcgaaaatt atacgaaaa tttgcaaag tccaggttt 
      acgtgaacg atgattttg gtagcaaaa aagtggtaa 
      agtcccaat gtcagaaca taaaacgcg ataaaaatg 
      acaacaatc gcacgaatt acagtcgct ataagcgag 
      atggcttag gggccccga atcgcattg ataaaaata 
      gcgagaaaa ttcgacaat ttgtcaaag aagggatta 
      acggtttta atgccaatt acaatagta aaaataaca 
      aagtgaagc cagttgaag tggtttcag caacaaccc 
      aagtcgttg cagggacac tttacaaaa tcacgacga 
      aaatttccg aatctgatt gttctaaaa acgataata 
      aagatttag atgattgta atattcttg aaggggctt 
      cgccaaaaa catgcgagt tggaggtgt gggagaaac 
      tgtgggaga atttcagac aattcacaa taaagtgtg 
      aaggggacg aacaaagta ttaaatttc aaacaagcc 
      ctaaaaata gagctaagc gtcaagctc ccggcggag 
      agtcaccgg tgaccaaag acaacgagt acgttgtta 
      agtatttgg aagcggctt taaaccaac tcgattctg 
      aatctccac acgagaata agtttaaag tccacgagt 
      ttatttctg ccacctcgc aaaccgtct cgggtcaca 
      tttatcgaa tcaaaacga aagtagtac tttccgatt 
      gtaaaaaaa ctgcttcga ccgagagag gtcaatgtg 
      ggaccctca aagatgcga aacccaaaa cttgcaaat 
      ttgaggttt ttgaacaaa cttgggttc ctaacagcc 
      gtcgaatta aaactgact gtgacaagg agaggagaa 
      aaaggaaaa cgttggacg gtggaataa cggaagtcg 
      ataaagacg acgatgaag tgaaaacat ttgttcgtg 
      aaggtttac tcaatttga atactcatt tgactacaa 
      gtaataaag tcaagcctg tagaagtgg tttctgctt 
      cagttcaag ttgtggcag ggtctttgc ataggatta 
      aagtgaaaa tatcagagt cggattgtt ccaggaacg 
      atcagaaag acttcgaac agtgtaatg tacttgagg 
      gggcttccc cgaaattgt gtgagatgg aggtttggg 
      ataaaccat gggaggact tcaggaggt acactataa 
      agtgtgaag gcgagaata agaaacacc agtttacgt 
      taaatccaa acaagcgaa ctaagcgtc aagttccca 
      ggggagaat caccagtgg cgaaagaca atgaatacg 
      tcattaaat atctggagg ctgctttaa atcaacttg 
      atgctgaat ctgaacacg aaaataagt ttaaagtcc 
      atgagttta tttcagcaa catcgcaga ttgtctccg 
      gtcacattt atcgcatca atgcgaaag tgatacttt 
      ccgattgta aaaaaacag tttcgaccg agagaggcc 
      agtgtggta ctctcaaag acgccaaac ccaaaactt 
      gcaaatttg aagtgtttg aacaacttt gggtaccta 
      atagtcgtc aaattaaaa ccgattgcc acaaagaga 
      gaagaaaaa ggcaaacat tgccaggtg gaataaagg 
      aagtcgata aaaacaacg atgaagtga aaacatttg 
      tacatgaag gtttactca atttgaata ctcatttga 
      ctacaagta acaaagtca agcctgtag aagtggttt 
      ctgcttcag ttcaagttg tggcagggt ctttgcata 
      ggattaaag tgaaaatat cagagtcgg attgttcca 
      ggaacgatc agaaagact tcgaacagt gtaatgtac 
      tcgaaggag cttccccga agttgtgtg agatggagg 
      tttgggata aaccatggg aggacttca ggaggtaca 
      ctataaagt gtgaaggcg aaaataaga aacatcagt 
      ttacgttaa atccaaaca agcgaacta agcgtcaag 
      ttcgggggg gagaatcac cagtggcga aagacaacg 
      aatacgtca ttaaatatc tggaggctg ctttaaatc 
      aacttgatg ctgaatctg aacacgaaa ataagttta 
      aagtccatg agtttattt cagcaacat cgcagattg 
      tctccggcc acatttatc gcatcaaag cgaaagtaa 
      tactttccg attgtaaaa aatcaattt cgactgaaa 
      gaggccagt gtggtactc tcaaagacg ccaaaccca 
      aaacttgca aatttgaag tgtttgaac agctttggg 
      tacctaata gtcgtcaaa ttaaaaccg agtgtgacg 
      gtaaaagag taaaaaggc aaaggagcg aaaaaccac 
      actccgtaa ggagtaaga ggcagtcaa tacctggtg 
      gagcaaccg aaattgata aaaaaagcg ataaagtca 
      agcaatacg tccgtgaaa gcttaactc acttaaaca 
      ctcaactga cttccagca ataaggtca agcctgtgg 
      aagtacttt ctgccacga gtcaagtcg ttgcaggaa 
      caatccata gaatcaaag taaaaatat ccgaatctg 
      attgttcta aagacgatg aaaaagact tcgatgagt 
      gtaacatcc gcgaaggtg cttccccga aaatatgcg 
      aagtaaagg tttgggata agccgtggc agaatttcc 
      gacagtaca atataacct gcgagggtg aggatcacc 
      actacacat tcgaaacaa aacccggaa aaacaaaac 
      gcagtgccg agttggaca atttattgg gttgcgacg 
      accgagaat accatttat ccctcttca ctgacttcc 
      tcaaaaaat acaacaaga aatatcaca agaaagaat 
      acaaataca gatttaatg tatttgtac aaaacttga 
      tgcaaatca gggtattga atacctttg aacagggga 
      cggcaacgt atggaatta cgaggtttg cggatatga 
      ctcaaaaag agttttcgc ggagtttgg gtttgcgta 
      cggatttga ggaacgaga atgagactc cgttcgcac 
      aagctaaaa ttcccaata ttgaacttc cgaaggagt 
      ttgattggc gaaagaaaa atgtcgtga cggaggtca 
      agaatcagg aacagtgtg gaagttgct gggcgttta 
      gcgtcacgg gaaatgtgg aagggcagt atgctttga 
      gacacggga agttgttgg agttttccg aacaggagt 
      tggtggact gtgatacgg atgaccaag ggtgtaatg 
      gagggttga tggacaccg cttacagat cgattgaga 
      aaatcggtg gcttggaga cagagcagg actacccct 
      acgacgccg aggacgaaa aatgccatt tcaatcgaa 
      ccttagccc gcgtccagg tcactgggg ccctcaaca 
      tcagccaca acgagaccg atatggcca agtggttgg 
      tggccaatg gccccattt ccatcgcca tcaatgcca 
      acgctatgc agttttaca tgggcgggg tgtcccatc 
      cattcaagt ttttgtgta gtccgaaaa accttgacc 
      acggggttt taatagtgg gttacggtg tacacaatt 
      atccactct tcaagaaga gtcttccgt attggatcg 
      tcaaaaact cgtggggga caggatggg gcgaacaag 
      gttactata gagtttaca ggggggacg gtacgtgtg 
      gtttgaacc agactccct ccagtgcca tagtagctt 
      aaagctcaa acatatcgc aactgaaca aatgtgata 
      tttttcaat gtctgtact tgcaacaac ttaattatg 
      gcggacgtg taaataaac tac