| General information | |||
| TC009844 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 8973617..8979501 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC009844 | |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01617 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
labrum bent - Penetrance (percentage of animals/eggs on slide): less than 30%
metathorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
abdomen partially constricted - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen complete not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC009844 RA
MATLWYMFV CFCWWSLVN SDVLQENLS NKSESHSSE ISAVLNHLN SSPFKVYNY NKATLLNAQ TKADIIELI LDIDVDCIN KYPNVPCGE KVVTCHAFV RNTPEEGSK VLTDVNCKP KPEIIVDEP VYDSPTNPT EVNTNEPTP IVLSNEAVS SEVGEHFVA VRKDSVPCL GCPFDLNTN AEGVDELID VALEHIQTE RAKKHALVK VLRLQQQVV TGVKYILTA EFAPTLCEK SANLDASSC PRDTNAETT ICEITYLHK PWISKAKHV IKNNCTVSQ EYVRPDIRK SDISNEIDV SGKRTKLID SPNFDKMAE NGAIDEMTP SRLADLESQ IIVDVEPEI GAPSTAESH PQVESKLEF DLFQTPESL TVSEDPAFT AGVTVEEPA PTEEHVSRL KRDESSEET KKDSSSESV ETAANQTKA TKTSSSEEN SSEEVLRPK RAIRQLEKI SPDEKGLVK NLADFAANA LDSIDDDNN KRIILQILG AKKMVGDDG VYYHIIMRM GVSRCLEDS PINPYENCK DKLFENYTK ICKVQVYVN DDFGSKKVV KSQCQNIKR DKNDNNRTN YSRYKRDGL GAPNRIDKN SEKIRQFVK EGINGFNAN YNSKNNKVK PVEVVSATT QVVAGTLYK ITTKISESD CSKNDNKDL DDCNILEGA SPKTCELEV WEKLWENFR QFTIKCEGD EQSIKFQTS PKNRAKRQA PGGESPVTK DNEYVVKYL EAALNQLDS ESPHENKFK VHEFISATS QTVSGHIYR IKTKVVLSD CKKTASTER GQCGTLKDA KPKTCKFEV FEQTWVPNS RRIKTDCDK ERRKRKTLD GGITEVDKD DDEVKTFVR EGLLNLNTH LTTSNKVKP VEVVSASVQ VVAGSLHRI KVKISESDC SRNDQKDFE QCNVLEGAS PKLCEMEVW DKPWEDFRR YTIKCEGEN KKHQFTLNP NKRTKRQVP RGESPVAKD NEYVIKYLE AALNQLDAE SEHENKFKV HEFISATSQ IVSGHIYRI NAKVILSDC KKTVSTERG QCGTLKDAK PKTCKFEVF EQLWVPNSR QIKTDCHKE RRKRQTLPG GIKEVDKNN DEVKTFVHE GLLNLNTHL TTSNKVKPV EVVSASVQV VAGSLHRIK VKISESDCS RNDQKDFEQ CNVLEGASP KLCEMEVWD KPWEDFRRY TIKCEGENK KHQFTLNPN KRTKRQVRG GESPVAKDN EYVIKYLEA ALNQLDAES EHENKFKVH EFISATSQI VSGHIYRIK AKVILSDCK KSISTERGQ CGTLKDAKP KTCKFEVFE QLWVPNSRQ IKTECDGKR VKRQRSEKP HSVRSKRQS IPGGATEID KKSDKVKQY VRESLTHLN TQLTSSNKV KPVEVLSAT SQVVAGTIH RIKVKISES DCSKDDEKD FDECNIREG ASPKICEVK VWDKPWQNF RQYNITCEG EDHHYTFET KPGKTKRSA ELDNLLGCD DREYHLSLF TDFLKKYNK KYHKKEYKY RFNVFVQNL MQIRVLNTF EQGTATYGI TRFADMTQK EFSRSLGLR TDLRNENET PFAQAKIPN IELPKEFDW RKKNVVTEV KNQEQCGSC WAFSVTGNV EGQYALRHG KLLEFSEQE LVDCDTDDQ GCNGGLMDT AYRSIEKIG GLETEQDYP YDAEDEKCH FNRTLARVQ VTGALNISH NETDMAKWL VANGPISIA INANAMQFY MGGVSHPFK FLCSPKNLD HGVLIVGYG VHNYPLFKK SLPYWIVKN SWGTGWGEQ GYYRVYRGD GTCGLNQTP SSAIVA
- >TC009844 RA
atggcgacc ttgtggtac atgtttgtt tgtttctgt tggtggtct ttagtaaat agtgatgtt ttacaagaa aatttatcg aacaaatca gaatctcat tcttccgaa atctcggca gtgttaaat cacctgaat tcgtctcct ttcaaggtg tacaactac aacaaagca accctccta aatgcgcag acaaaggct gatatcatc gaattaatt ctcgacata gatgtggat tgcatcaac aagtacccc aatgtgccc tgcggcgaa aaagtcgtc acttgtcac gccttcgtc aggaatacg cctgaagaa ggcagcaag gtgctcact gatgtcaat tgcaaacct aaacctgag attatcgtt gatgagcca gtgtatgac tcaccaaca aacccaacc gaagtgaac acaaatgaa ccaacacct attgtgtta agcaacgaa gctgtttcg tctgaagtg ggcgagcat ttcgtcgcg gtcaggaaa gactcagtt ccttgttta ggctgcccc tttgacctc aacacaaat gcggaaggc gtggatgaa ctaattgac gtagcttta gagcacatt cagacggaa cgtgccaaa aaacatgcg ctggtcaaa gtactcaga ttacaacaa caagttgta actggagtt aaatatatc ctgacggcg gaattcgcg cccacactt tgcgaaaaa agtgcaaac cttgacgcg tcttcatgc ccaagagac acaaatgca gaaacaaca atttgcgag atcacctac ttgcataaa ccttggata agtaaagcc aaacacgtg atcaaaaat aattgtacc gtttcgcaa gaatacgtc aggcccgat attaggaag tctgatata agtaatgaa attgatgtt agcggtaaa cgtactaaa ctaattgac tctcccaat tttgataaa atggcagaa aacggggct attgacgaa atgacaccc tcgcggtta gcggatttg gagtcacaa ataatcgtt gatgtggag cctgaaatc ggagcgccc tccactgcg gagtcacac cctcaggta gagagcaaa cttgagttt gatttattt caaacacct gaaagtctg acggtgtct gaagatccc gcatttacg gcgggtgtt accgtggag gagcctgcg cccactgaa gaacatgtt tcgaggttg aaacgtgac gaaagttca gaagaaact aagaaagat tcttcaagt gaatcagtc gaaacggca gcaaaccaa accaaagct acgaaaacc agcagctct gaggaaaat tcaagtgag gaggtttta agaccaaag agagcaatc cgtcagttg gagaaaatc tcgcctgat gagaaaggc ttggtgaag aatttggcc gactttgcc gccaatgcg ctagacagc attgacgat gataacaac aaacgaata attttgcaa attttgggg gcgaaaaaa atggtcggg gacgatggg gtttattat cacattatc atgcggatg ggagtctcg cgttgcttg gaggatagc ccaatcaat ccgtacgaa aattgcaag gacaagctt ttcgaaaat tatacgaaa atttgcaaa gtccaggtt tacgtgaac gatgatttt ggtagcaaa aaagtggta aagtcccaa tgtcagaac ataaaacgc gataaaaat gacaacaat cgcacgaat tacagtcgc tataagcga gatggctta ggggccccg aatcgcatt gataaaaat agcgagaaa attcgacaa tttgtcaaa gaagggatt aacggtttt aatgccaat tacaatagt aaaaataac aaagtgaag ccagttgaa gtggtttca gcaacaacc caagtcgtt gcagggaca ctttacaaa atcacgacg aaaatttcc gaatctgat tgttctaaa aacgataat aaagattta gatgattgt aatattctt gaaggggct tcgccaaaa acatgcgag ttggaggtg tgggagaaa ctgtgggag aatttcaga caattcaca ataaagtgt gaaggggac gaacaaagt attaaattt caaacaagc cctaaaaat agagctaag cgtcaagct cccggcgga gagtcaccg gtgaccaaa gacaacgag tacgttgtt aagtatttg gaagcggct ttaaaccaa ctcgattct gaatctcca cacgagaat aagtttaaa gtccacgag tttatttct gccacctcg caaaccgtc tcgggtcac atttatcga atcaaaacg aaagtagta ctttccgat tgtaaaaaa actgcttcg accgagaga ggtcaatgt gggaccctc aaagatgcg aaacccaaa acttgcaaa tttgaggtt tttgaacaa acttgggtt cctaacagc cgtcgaatt aaaactgac tgtgacaag gagaggaga aaaaggaaa acgttggac ggtggaata acggaagtc gataaagac gacgatgaa gtgaaaaca tttgttcgt gaaggttta ctcaatttg aatactcat ttgactaca agtaataaa gtcaagcct gtagaagtg gtttctgct tcagttcaa gttgtggca gggtctttg cataggatt aaagtgaaa atatcagag tcggattgt tccaggaac gatcagaaa gacttcgaa cagtgtaat gtacttgag ggggcttcc ccgaaattg tgtgagatg gaggtttgg gataaacca tgggaggac ttcaggagg tacactata aagtgtgaa ggcgagaat aagaaacac cagtttacg ttaaatcca aacaagcga actaagcgt caagttccc aggggagaa tcaccagtg gcgaaagac aatgaatac gtcattaaa tatctggag gctgcttta aatcaactt gatgctgaa tctgaacac gaaaataag tttaaagtc catgagttt atttcagca acatcgcag attgtctcc ggtcacatt tatcgcatc aatgcgaaa gtgatactt tccgattgt aaaaaaaca gtttcgacc gagagaggc cagtgtggt actctcaaa gacgccaaa cccaaaact tgcaaattt gaagtgttt gaacaactt tgggtacct aatagtcgt caaattaaa accgattgc cacaaagag agaagaaaa aggcaaaca ttgccaggt ggaataaag gaagtcgat aaaaacaac gatgaagtg aaaacattt gtacatgaa ggtttactc aatttgaat actcatttg actacaagt aacaaagtc aagcctgta gaagtggtt tctgcttca gttcaagtt gtggcaggg tctttgcat aggattaaa gtgaaaata tcagagtcg gattgttcc aggaacgat cagaaagac ttcgaacag tgtaatgta ctcgaagga gcttccccg aagttgtgt gagatggag gtttgggat aaaccatgg gaggacttc aggaggtac actataaag tgtgaaggc gaaaataag aaacatcag tttacgtta aatccaaac aagcgaact aagcgtcaa gttcggggg ggagaatca ccagtggcg aaagacaac gaatacgtc attaaatat ctggaggct gctttaaat caacttgat gctgaatct gaacacgaa aataagttt aaagtccat gagtttatt tcagcaaca tcgcagatt gtctccggc cacatttat cgcatcaaa gcgaaagta atactttcc gattgtaaa aaatcaatt tcgactgaa agaggccag tgtggtact ctcaaagac gccaaaccc aaaacttgc aaatttgaa gtgtttgaa cagctttgg gtacctaat agtcgtcaa attaaaacc gagtgtgac ggtaaaaga gtaaaaagg caaaggagc gaaaaacca cactccgta aggagtaag aggcagtca atacctggt ggagcaacc gaaattgat aaaaaaagc gataaagtc aagcaatac gtccgtgaa agcttaact cacttaaac actcaactg acttccagc aataaggtc aagcctgtg gaagtactt tctgccacg agtcaagtc gttgcagga acaatccat agaatcaaa gtaaaaata tccgaatct gattgttct aaagacgat gaaaaagac ttcgatgag tgtaacatc cgcgaaggt gcttccccg aaaatatgc gaagtaaag gtttgggat aagccgtgg cagaatttc cgacagtac aatataacc tgcgagggt gaggatcac cactacaca ttcgaaaca aaacccgga aaaacaaaa cgcagtgcc gagttggac aatttattg ggttgcgac gaccgagaa taccattta tccctcttc actgacttc ctcaaaaaa tacaacaag aaatatcac aagaaagaa tacaaatac agatttaat gtatttgta caaaacttg atgcaaatc agggtattg aataccttt gaacagggg acggcaacg tatggaatt acgaggttt gcggatatg actcaaaaa gagttttcg cggagtttg ggtttgcgt acggatttg aggaacgag aatgagact ccgttcgca caagctaaa attcccaat attgaactt ccgaaggag tttgattgg cgaaagaaa aatgtcgtg acggaggtc aagaatcag gaacagtgt ggaagttgc tgggcgttt agcgtcacg ggaaatgtg gaagggcag tatgctttg agacacggg aagttgttg gagttttcc gaacaggag ttggtggac tgtgatacg gatgaccaa gggtgtaat ggagggttg atggacacc gcttacaga tcgattgag aaaatcggt ggcttggag acagagcag gactacccc tacgacgcc gaggacgaa aaatgccat ttcaatcga accttagcc cgcgtccag gtcactggg gccctcaac atcagccac aacgagacc gatatggcc aagtggttg gtggccaat ggccccatt tccatcgcc atcaatgcc aacgctatg cagttttac atgggcggg gtgtcccat ccattcaag tttttgtgt agtccgaaa aaccttgac cacggggtt ttaatagtg ggttacggt gtacacaat tatccactc ttcaagaag agtcttccg tattggatc gtcaaaaac tcgtggggg acaggatgg ggcgaacaa ggttactat agagtttac aggggggac ggtacgtgt ggtttgaac cagactccc tccagtgcc atagtagct taa
- >TC009844 RA
ctgattttg acacttttc agctttgac atcaatcag aaacaaaca tggcgacct tgtggtaca tgtttgttt gtttctgtt ggtggtctt tagtaaata gtgatgttt tacaagaaa atttatcga acaaatcag aatctcatt cttccgaaa tctcggcag tgttaaatc acctgaatt cgtctcctt tcaaggtgt acaactaca acaaagcaa ccctcctaa atgcgcaga caaaggctg atatcatcg aattaattc tcgacatag atgtggatt gcatcaaca agtacccca atgtgccct gcggcgaaa aagtcgtca cttgtcacg ccttcgtca ggaatacgc ctgaagaag gcagcaagg tgctcactg atgtcaatt gcaaaccta aacctgaga ttatcgttg atgagccag tgtatgact caccaacaa acccaaccg aagtgaaca caaatgaac caacaccta ttgtgttaa gcaacgaag ctgtttcgt ctgaagtgg gcgagcatt tcgtcgcgg tcaggaaag actcagttc cttgtttag gctgcccct ttgacctca acacaaatg cggaaggcg tggatgaac taattgacg tagctttag agcacattc agacggaac gtgccaaaa aacatgcgc tggtcaaag tactcagat tacaacaac aagttgtaa ctggagtta aatatatcc tgacggcgg aattcgcgc ccacacttt gcgaaaaaa gtgcaaacc ttgacgcgt cttcatgcc caagagaca caaatgcag aaacaacaa tttgcgaga tcacctact tgcataaac cttggataa gtaaagcca aacacgtga tcaaaaata attgtaccg tttcgcaag aatacgtca ggcccgata ttaggaagt ctgatataa gtaatgaaa ttgatgtta gcggtaaac gtactaaac taattgact ctcccaatt ttgataaaa tggcagaaa acggggcta ttgacgaaa tgacaccct cgcggttag cggatttgg agtcacaaa taatcgttg atgtggagc ctgaaatcg gagcgccct ccactgcgg agtcacacc ctcaggtag agagcaaac ttgagtttg atttatttc aaacacctg aaagtctga cggtgtctg aagatcccg catttacgg cgggtgtta ccgtggagg agcctgcgc ccactgaag aacatgttt cgaggttga aacgtgacg aaagttcag aagaaacta agaaagatt cttcaagtg aatcagtcg aaacggcag caaaccaaa ccaaagcta cgaaaacca gcagctctg aggaaaatt caagtgagg aggttttaa gaccaaaga gagcaatcc gtcagttgg agaaaatct cgcctgatg agaaaggct tggtgaaga atttggccg actttgccg ccaatgcgc tagacagca ttgacgatg ataacaaca aacgaataa ttttgcaaa ttttggggg cgaaaaaaa tggtcgggg acgatgggg tttattatc acattatca tgcggatgg gagtctcgc gttgcttgg aggatagcc caatcaatc cgtacgaaa attgcaagg acaagcttt tcgaaaatt atacgaaaa tttgcaaag tccaggttt acgtgaacg atgattttg gtagcaaaa aagtggtaa agtcccaat gtcagaaca taaaacgcg ataaaaatg acaacaatc gcacgaatt acagtcgct ataagcgag atggcttag gggccccga atcgcattg ataaaaata gcgagaaaa ttcgacaat ttgtcaaag aagggatta acggtttta atgccaatt acaatagta aaaataaca aagtgaagc cagttgaag tggtttcag caacaaccc aagtcgttg cagggacac tttacaaaa tcacgacga aaatttccg aatctgatt gttctaaaa acgataata aagatttag atgattgta atattcttg aaggggctt cgccaaaaa catgcgagt tggaggtgt gggagaaac tgtgggaga atttcagac aattcacaa taaagtgtg aaggggacg aacaaagta ttaaatttc aaacaagcc ctaaaaata gagctaagc gtcaagctc ccggcggag agtcaccgg tgaccaaag acaacgagt acgttgtta agtatttgg aagcggctt taaaccaac tcgattctg aatctccac acgagaata agtttaaag tccacgagt ttatttctg ccacctcgc aaaccgtct cgggtcaca tttatcgaa tcaaaacga aagtagtac tttccgatt gtaaaaaaa ctgcttcga ccgagagag gtcaatgtg ggaccctca aagatgcga aacccaaaa cttgcaaat ttgaggttt ttgaacaaa cttgggttc ctaacagcc gtcgaatta aaactgact gtgacaagg agaggagaa aaaggaaaa cgttggacg gtggaataa cggaagtcg ataaagacg acgatgaag tgaaaacat ttgttcgtg aaggtttac tcaatttga atactcatt tgactacaa gtaataaag tcaagcctg tagaagtgg tttctgctt cagttcaag ttgtggcag ggtctttgc ataggatta aagtgaaaa tatcagagt cggattgtt ccaggaacg atcagaaag acttcgaac agtgtaatg tacttgagg gggcttccc cgaaattgt gtgagatgg aggtttggg ataaaccat gggaggact tcaggaggt acactataa agtgtgaag gcgagaata agaaacacc agtttacgt taaatccaa acaagcgaa ctaagcgtc aagttccca ggggagaat caccagtgg cgaaagaca atgaatacg tcattaaat atctggagg ctgctttaa atcaacttg atgctgaat ctgaacacg aaaataagt ttaaagtcc atgagttta tttcagcaa catcgcaga ttgtctccg gtcacattt atcgcatca atgcgaaag tgatacttt ccgattgta aaaaaacag tttcgaccg agagaggcc agtgtggta ctctcaaag acgccaaac ccaaaactt gcaaatttg aagtgtttg aacaacttt gggtaccta atagtcgtc aaattaaaa ccgattgcc acaaagaga gaagaaaaa ggcaaacat tgccaggtg gaataaagg aagtcgata aaaacaacg atgaagtga aaacatttg tacatgaag gtttactca atttgaata ctcatttga ctacaagta acaaagtca agcctgtag aagtggttt ctgcttcag ttcaagttg tggcagggt ctttgcata ggattaaag tgaaaatat cagagtcgg attgttcca ggaacgatc agaaagact tcgaacagt gtaatgtac tcgaaggag cttccccga agttgtgtg agatggagg tttgggata aaccatggg aggacttca ggaggtaca ctataaagt gtgaaggcg aaaataaga aacatcagt ttacgttaa atccaaaca agcgaacta agcgtcaag ttcgggggg gagaatcac cagtggcga aagacaacg aatacgtca ttaaatatc tggaggctg ctttaaatc aacttgatg ctgaatctg aacacgaaa ataagttta aagtccatg agtttattt cagcaacat cgcagattg tctccggcc acatttatc gcatcaaag cgaaagtaa tactttccg attgtaaaa aatcaattt cgactgaaa gaggccagt gtggtactc tcaaagacg ccaaaccca aaacttgca aatttgaag tgtttgaac agctttggg tacctaata gtcgtcaaa ttaaaaccg agtgtgacg gtaaaagag taaaaaggc aaaggagcg aaaaaccac actccgtaa ggagtaaga ggcagtcaa tacctggtg gagcaaccg aaattgata aaaaaagcg ataaagtca agcaatacg tccgtgaaa gcttaactc acttaaaca ctcaactga cttccagca ataaggtca agcctgtgg aagtacttt ctgccacga gtcaagtcg ttgcaggaa caatccata gaatcaaag taaaaatat ccgaatctg attgttcta aagacgatg aaaaagact tcgatgagt gtaacatcc gcgaaggtg cttccccga aaatatgcg aagtaaagg tttgggata agccgtggc agaatttcc gacagtaca atataacct gcgagggtg aggatcacc actacacat tcgaaacaa aacccggaa aaacaaaac gcagtgccg agttggaca atttattgg gttgcgacg accgagaat accatttat ccctcttca ctgacttcc tcaaaaaat acaacaaga aatatcaca agaaagaat acaaataca gatttaatg tatttgtac aaaacttga tgcaaatca gggtattga atacctttg aacagggga cggcaacgt atggaatta cgaggtttg cggatatga ctcaaaaag agttttcgc ggagtttgg gtttgcgta cggatttga ggaacgaga atgagactc cgttcgcac aagctaaaa ttcccaata ttgaacttc cgaaggagt ttgattggc gaaagaaaa atgtcgtga cggaggtca agaatcagg aacagtgtg gaagttgct gggcgttta gcgtcacgg gaaatgtgg aagggcagt atgctttga gacacggga agttgttgg agttttccg aacaggagt tggtggact gtgatacgg atgaccaag ggtgtaatg gagggttga tggacaccg cttacagat cgattgaga aaatcggtg gcttggaga cagagcagg actacccct acgacgccg aggacgaaa aatgccatt tcaatcgaa ccttagccc gcgtccagg tcactgggg ccctcaaca tcagccaca acgagaccg atatggcca agtggttgg tggccaatg gccccattt ccatcgcca tcaatgcca acgctatgc agttttaca tgggcgggg tgtcccatc cattcaagt ttttgtgta gtccgaaaa accttgacc acggggttt taatagtgg gttacggtg tacacaatt atccactct tcaagaaga gtcttccgt attggatcg tcaaaaact cgtggggga caggatggg gcgaacaag gttactata gagtttaca ggggggacg gtacgtgtg gtttgaacc agactccct ccagtgcca tagtagctt aaagctcaa acatatcgc aactgaaca aatgtgata tttttcaat gtctgtact tgcaacaac ttaattatg gcggacgtg taaataaac tac
