Status
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Gene information
General information
TC009820 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8621706..8626583
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009820
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01615

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009820 RA
      MSNLIPPLL SSSPPPIVG PLDEDDDDE FGDFRAAAD 
      SFDCDDFSL PPSPQKSSE LKTDLSKVS LDINSNKSQ 
      KNTQEMPTE NVEDSVVCE EVSKDFQDR RKSLDAEIK 
      VNSSEQIDE INGFEADFN DQKIEDDDF ADLQCTLPN 
      ETQVENGVN SLSNDENLF EAFSEKTND ISESNEIKS 
      EDYFSEEIN DKCENDLSE SSVSLKVSE SEQKSTEDD 
      FGDFENHFS NDKEASDDE FDDFVEPPQ QSKEDDDFG 
      DFESSEFGD FSQSCEKST LPEIDVKNA LEKSEAILK 
      EFFPDIEQD SDDCVAADY TTENRIFDE LKDVTETNA 
      LVYQWSKSN SQKMFLKSL NIDTRNILY GPAWNGSMP 
      RFAGLTPLE PVKTEILTP TPVQNVSSP SSNSSQKEI 
      SDIPTVQFD WNGSGLINP LDSTSTELS ANVPEETTQ 
      SQNSDFLPP VSSRPDLFE PELQVSKEN EKTDFSPFH 
      SPKLIPLRE THISNEQTD LSKPGWLQP TILTPDLPR 
      KEPVIAEPE DEFDDFQMV LPEENKNLP DLSEFETTK 
      TNSQHISAS ETSGPAAEE VIEEDEFTE FHASLPVES 
      LQSVQPEPL KPVQPELPK SVLPLESLK PLQPEPLKP 
      VLPAEPFKP VQPEPLKPV LPAEPFKPV QPEPLKPVL 
      PMEPFKPVQ PEPLKPVLP LEPLKPLPV EPLKPVPVE 
      PLKPSSTLM TQINWPEPG VTDEDIRNI ELTYSRKEE 
      KPEISKGKS LDDDEWSDF VSVTNPVMK PVMPDRTAT 
      PDLPLSVLN LNSVQAPKQ PIPVITPSG LVQTKLSSN 
      MPKSVVQTS VQPMFRPPM NQFQPSIIS NQFAANFPG 
      ASQDDDDDW SDFVSSQPP PQVNGYQKA APTNIIMNP 
      THFETLNYT APKNKISTI PDLDFIAPK NRTKK
    CDS sequence
    • >TC009820 RA
      atgtcgaat ttgattccg ccgcttttg agcagttca 
      ccacctcca attgtaggg ccacttgac gaggatgac 
      gacgacgaa tttggggat tttcgggcc gctgctgat 
      tcgtttgac tgtgatgat ttctcttta ccaccttca 
      ccacaaaaa tcctcagaa ctgaaaacc gacctttcc 
      aaagtcagt ttagatata aatagcaac aagagccaa 
      aaaaacaca caagaaatg ccaactgag aacgtagaa 
      gactccgta gtgtgtgag gaagtcagt aaagatttt 
      caagacagg agaaaaagc ttagatgct gagatcaaa 
      gtcaattct agtgaacaa attgatgaa ataaatggc 
      tttgaggcc gactttaat gaccaaaaa attgaagat 
      gatgatttc gctgatctt caatgtact ctaccaaat 
      gaaactcaa gtagaaaat ggagtaaat agcctcagc 
      aatgacgaa aatctattt gaggctttt agtgaaaag 
      acaaatgac atttctgaa agtaatgag attaaatct 
      gaggattac ttctctgaa gagattaac gacaaatgt 
      gagaacgat ctgagcgag tcctcagtt agtctcaaa 
      gtatctgaa agtgaacaa aaatcaact gaagacgat 
      ttcggcgac tttgaaaac cattttagt aacgataag 
      gaggcatca gacgacgaa ttcgacgat tttgttgaa 
      cctccgcaa cagagtaaa gaagatgac gatttcggc 
      gattttgaa tcgtcagaa ttcggcgat ttttcgcaa 
      agttgcgaa aagtcgact cttcccgag attgacgtg 
      aaaaatgct cttgaaaaa agcgaagca attttgaag 
      gaatttttc cccgatatc gaacaagac agtgacgat 
      tgcgtcgct gctgattac acaacagaa aatcgcatt 
      tttgacgaa ctgaaggat gttacggaa actaacgct 
      ctggtgtat cagtggtcg aagtcaaac agccagaaa 
      atgttcctt aaatcattg aatatcgat acaagaaat 
      atcttgtat ggccccgct tggaacggt tcgatgccg 
      cgattcgcc ggtttgacg ccactggag ccggttaaa 
      accgaaata ctcactcca acgccggtt caaaacgtt 
      tcgagtccg tccagtaat tctagtcag aaggaaatt 
      agtgatatt ccaacggtt cagttcgat tggaacggc 
      tcgggattg ataaatcct cttgattcc acgtcaacc 
      gaactgtca gccaacgta cctgaagaa acaacccag 
      tcacaaaat tccgatttc ttgcctccg gtgtcatcc 
      agaccggat ttgttcgaa ccggaactt caagtatcc 
      aaagaaaat gaaaaaacc gacttttcg ccattccat 
      tcgcctaaa ctaataccc cttcgtgag acccacatt 
      tcgaacgaa caaaccgat ttgagcaaa ccgggatgg 
      ttacaaccg acgattctg acaccagat ttaccgcga 
      aaagaacca gttatagcc gaacccgaa gatgaattt 
      gacgatttc caaatggtt ttacccgaa gaaaacaaa 
      aatttgccc gacttgagc gaattcgaa acgaccaaa 
      actaatagt caacatatc tctgcaagt gaaactagc 
      ggaccagca gcagaggaa gtgattgaa gaagacgag 
      tttacggag tttcatgcg tcacttcct gtggaatct 
      cttcagtcg gtccaacca gagccgcta aaaccggtc 
      cagcctgag ctgccaaaa tcggttctt ccgctggaa 
      tcgcttaaa ccgctccag cctgagccg ctaaaaccg 
      gttcttcca gcagaaccg tttaaaccg gtccagcct 
      gagccgtta aaaccggtt cttccagca gaaccgttt 
      aaaccggtc cagcctgag ccgttaaaa ccggttctt 
      ccaatggag ccgtttaaa ccggtccaa cccgagccg 
      ttaaagccg gttcttccg ctcgaaccg ttaaaaccc 
      ctacccgtc gagccgtta aaaccggtt ccggtcgag 
      ccgttaaaa ccaagttcg actctaatg acgcagata 
      aattggccg gagcccgga gtcaccgat gaagacatc 
      agaaacatc gagttgact tactcccgc aaagaggag 
      aaaccggaa attagtaaa ggaaagtcg cttgatgac 
      gacgaatgg agtgatttt gtttcggtt acaaatccg 
      gttatgaag ccggttatg cccgaccgg actgcgact 
      ccggacttg cctttgtcg gttcttaat ttgaatagt 
      gttcaagcc ccgaaacaa ccgattccg gttataacc 
      cctagcggt ttggttcag acgaagttg tcgtcgaat 
      atgccaaaa tcagtggtt caaactagt gttcaacca 
      atgtttcgg cccccaatg aatcagttt cagccctct 
      attattagt aatcagttc gcggcgaat tttccgggg 
      gctagtcaa gacgatgac gatgattgg agcgatttt 
      gtgtcaagt cagccgccc cctcaagtc aacgggtat 
      cagaaggca gcccccacg aacattatc atgaatccg 
      acgcatttc gagacgttg aattatacc gcaccgaaa 
      aataaaatt tcaacaatc cctgatttg gatttcata 
      gctcccaaa aatcggact aaaaaatga
    mRNA sequence
    • >TC009820 RA
      tgacaatga catgtaatt agtaacgtc aaaatggct 
      ggttgtcat ttgtgttaa aaaaataaa tttgcgtct 
      taaagtgaa taactaaca gccaggacg ttccaactg 
      attgcaaat tgtgcggtg cttggtggt tggtcgttg 
      ttaatttaa gagtgtgcg agaaacata aatttggtt 
      gttttatta ttcaacaca ggtgacata ttttccatc 
      atttctcat tcagatgtc gaatttgat tccgccgct 
      tttgagcag ttcaccacc tccaattgt agggccact 
      tgacgagga tgacgacga cgaatttgg ggattttcg 
      ggccgctgc tgattcgtt tgactgtga tgatttctc 
      tttaccacc ttcaccaca aaaatcctc agaactgaa 
      aaccgacct ttccaaagt cagtttaga tataaatag 
      caacaagag ccaaaaaaa cacacaaga aatgccaac 
      tgagaacgt agaagactc cgtagtgtg tgaggaagt 
      cagtaaaga ttttcaaga caggagaaa aagcttaga 
      tgctgagat caaagtcaa ttctagtga acaaattga 
      tgaaataaa tggctttga ggccgactt taatgacca 
      aaaaattga agatgatga tttcgctga tcttcaatg 
      tactctacc aaatgaaac tcaagtaga aaatggagt 
      aaatagcct cagcaatga cgaaaatct atttgaggc 
      ttttagtga aaagacaaa tgacatttc tgaaagtaa 
      tgagattaa atctgagga ttacttctc tgaagagat 
      taacgacaa atgtgagaa cgatctgag cgagtcctc 
      agttagtct caaagtatc tgaaagtga acaaaaatc 
      aactgaaga cgatttcgg cgactttga aaaccattt 
      tagtaacga taaggaggc atcagacga cgaattcga 
      cgattttgt tgaacctcc gcaacagag taaagaaga 
      tgacgattt cggcgattt tgaatcgtc agaattcgg 
      cgatttttc gcaaagttg cgaaaagtc gactcttcc 
      cgagattga cgtgaaaaa tgctcttga aaaaagcga 
      agcaatttt gaaggaatt tttccccga tatcgaaca 
      agacagtga cgattgcgt cgctgctga ttacacaac 
      agaaaatcg catttttga cgaactgaa ggatgttac 
      ggaaactaa cgctctggt gtatcagtg gtcgaagtc 
      aaacagcca gaaaatgtt ccttaaatc attgaatat 
      cgatacaag aaatatctt gtatggccc cgcttggaa 
      cggttcgat gccgcgatt cgccggttt gacgccact 
      ggagccggt taaaaccga aatactcac tccaacgcc 
      ggttcaaaa cgtttcgag tccgtccag taattctag 
      tcagaagga aattagtga tattccaac ggttcagtt 
      cgattggaa cggctcggg attgataaa tcctcttga 
      ttccacgtc aaccgaact gtcagccaa cgtacctga 
      agaaacaac ccagtcaca aaattccga tttcttgcc 
      tccggtgtc atccagacc ggatttgtt cgaaccgga 
      acttcaagt atccaaaga aaatgaaaa aaccgactt 
      ttcgccatt ccattcgcc taaactaat accccttcg 
      tgagaccca catttcgaa cgaacaaac cgatttgag 
      caaaccggg atggttaca accgacgat tctgacacc 
      agatttacc gcgaaaaga accagttat agccgaacc 
      cgaagatga atttgacga tttccaaat ggttttacc 
      cgaagaaaa caaaaattt gcccgactt gagcgaatt 
      cgaaacgac caaaactaa tagtcaaca tatctctgc 
      aagtgaaac tagcggacc agcagcaga ggaagtgat 
      tgaagaaga cgagtttac ggagtttca tgcgtcact 
      tcctgtgga atctcttca gtcggtcca accagagcc 
      gctaaaacc ggtccagcc tgagctgcc aaaatcggt 
      tcttccgct ggaatcgct taaaccgct ccagcctga 
      gccgctaaa accggttct tccagcaga accgtttaa 
      accggtcca gcctgagcc gttaaaacc ggttcttcc 
      agcagaacc gtttaaacc ggtccagcc tgagccgtt 
      aaaaccggt tcttccaat ggagccgtt taaaccggt 
      ccaacccga gccgttaaa gccggttct tccgctcga 
      accgttaaa acccctacc cgtcgagcc gttaaaacc 
      ggttccggt cgagccgtt aaaaccaag ttcgactct 
      aatgacgca gataaattg gccggagcc cggagtcac 
      cgatgaaga catcagaaa catcgagtt gacttactc 
      ccgcaaaga ggagaaacc ggaaattag taaaggaaa 
      gtcgcttga tgacgacga atggagtga ttttgtttc 
      ggttacaaa tccggttat gaagccggt tatgcccga 
      ccggactgc gactccgga cttgccttt gtcggttct 
      taatttgaa tagtgttca agccccgaa acaaccgat 
      tccggttat aacccctag cggtttggt tcagacgaa 
      gttgtcgtc gaatatgcc aaaatcagt ggttcaaac 
      tagtgttca accaatgtt tcggccccc aatgaatca 
      gtttcagcc ctctattat tagtaatca gttcgcggc 
      gaattttcc gggggctag tcaagacga tgacgatga 
      ttggagcga ttttgtgtc aagtcagcc gccccctca 
      agtcaacgg gtatcagaa ggcagcccc cacgaacat 
      tatcatgaa tccgacgca tttcgagac gttgaatta 
      taccgcacc gaaaaataa aatttcaac aatccctga 
      tttggattt catagctcc caaaaatcg gactaaaaa 
      atgactaag ggagcaagt tatgcttta agcagtatt 
      tcgaaaggg gcatgagtg atatttatt tctgtctag 
      ggaagaaaa agtattttc gttacgtac ttaattatg 
      tttattgtt cacgtccat gtatataaa gtattgtta 
      tttatcatt ccagattgt atataaata aaaatcatg 
      tttaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY