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Gene information
General information
TC009816 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 1885188..1887004
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009816
    iBeetle screen iB_01614
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    tropharium irregular - Number of animals showing the phenotype: 4

    previtellogenic egg chamber not present - Number of animals showing the phenotype: 4
    vitellogenic egg chamber degenerated - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection


    Pupal morphology
    eyes sometimes shape irregular - Number of animals showing the phenotype: 4

    Comment: Omatidia affected, but defect not clearly visible. Gena affected.
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009816 RA
      MAEYKSEKI LISFMCQRC SQPIKLDDS LSSFSEHIS 
      AELNLPIHS NPDVDLESQ ATSFDQYIP ACRLVDSGN 
      GFMLISDEN EVDSLSHAY KKKAALFDT LSGNSNIDH 
      PLCDECTDY LLEILEQEL IDTQTDYDD YLRYYKMLQ 
      NDTNEPKLS ELEKELEDL KAEGNRFLE ELEALKKEE 
      IELQNAIKE QEAEARRLD KERNIYYKE YTKHQKNYT 
      QACEDAKSL ECQLNYTQI QLDKLKKTN VFNATFHIW 
      HKGHFGTIN NFCLGRLPS APVDWSEIN AAWGQTALL 
      LSALARKIN LTFERYRLV PYGNHSYIE VIGEQKEFP 
      LYGSGGFKF LWDTKFDNG MAAFLDCLQ QFQEKVEKL 
      EKGNKQFCF PYRTNKGKI EDNDASYSI KIQLNSEEQ 
      WTKAMKFML TNLKWGLAW ISSQLNNDI EENMQTEN
    CDS sequence
    • >TC009816 RA
      atggcagaa tacaaaagt gaaaaaatc ttaatatcc 
      tttatgtgc caaaggtgt tcacaaccg ataaaatta 
      gatgattct ttaagttca ttcagtgaa catatttca 
      gctgaactg aatttgccc atacattcc aatccagat 
      gtagattta gaatcccaa gctacgagt tttgatcag 
      tatatccct gcatgtcga ctggttgat tcaggaaat 
      ggtttcatg ctgatatca gatgaaaac gaagtagat 
      tcacttagt cacgcttat aagaaaaaa gcagcttta 
      tttgatact ctctcaggc aactcaaat attgatcac 
      cctttgtgt gacgaatgt acagattat ttattagaa 
      atactagaa caagaatta atagataca caaaccgat 
      tacgatgat tacctcaga tattacaaa atgctccag 
      aacgatacg aacgaacca aaactctca gaactggaa 
      aaggagttg gaagatttg aaagctgaa ggcaacagg 
      tttttggaa gaattggaa gccttgaaa aaggaagaa 
      attgagtta caaaacgca atcaaggaa caagaagca 
      gaagctagg agacttgac aaggaacgc aatatctac 
      tacaaagag tacaccaag catcaaaag aattatact 
      caagcttgc gaagatgct aagagtttg gagtgccag 
      ttgaattac actcaaatc caactcgac aaactcaag 
      aaaaccaac gtcttcaac gcaacgttc cacatctgg 
      cacaaaggc cacttcggc accatcaac aatttctgc 
      cttggaagg ctgccttcg gctcctgtc gactggtct 
      gaaatcaac gcagcttgg ggtcaaact gcactcttg 
      ctctctgca cttgcacgc aaaataaat ctgaccttt 
      gaaagatac agattagtg ccatacggc aatcactcg 
      tacattgag gtaattggc gaacaaaaa gagttccct 
      ttgtacggt tcgggcggt tttaagttt ttgtgggac 
      actaagttt gacaacgga atggccgcg tttttagat 
      tgcttacaa cagtttcag gaaaaggtg gagaaattg 
      gaaaagggg aataaacag ttttgtttt ccgtatcga 
      acaaataag gggaaaatt gaggacaat gacgcgagt 
      tactcaata aaaattcag ttaaattct gaggaacaa 
      tggaccaaa gcaatgaaa tttatgtta actaatttg 
      aagtggggt ctagcttgg atttcatca cagttaaat 
      aacgatata gaagagaat atgcaaacg gaaaattag
    mRNA sequence
    • >TC009816 RA
      tgtttaacg ccaaacaac gatcataaa aggaaagtc 
      tatttactt gtgataaat ctgcgtaaa atgtgtaaa 
      acaaagtgt taaaatggc agaatacaa aagtgaaaa 
      aatcttaat atcctttat gtgccaaag gtgttcaca 
      accgataaa attagatga ttctttaag ttcattcag 
      tgaacatat ttcagctga actgaattt gcccataca 
      ttccaatcc agatgtaga tttagaatc ccaagctac 
      gagttttga tcagtatat ccctgcatg tcgactggt 
      tgattcagg aaatggttt catgctgat atcagatga 
      aaacgaagt agattcact tagtcacgc ttataagaa 
      aaaagcagc tttatttga tactctctc aggcaactc 
      aaatattga tcacccttt gtgtgacga atgtacaga 
      ttatttatt agaaatact agaacaaga attaataga 
      tacacaaac cgattacga tgattacct cagatatta 
      caaaatgct ccagaacga tacgaacga accaaaact 
      ctcagaact ggaaaagga gttggaaga tttgaaagc 
      tgaaggcaa caggttttt ggaagaatt ggaagcctt 
      gaaaaagga agaaattga gttacaaaa cgcaatcaa 
      ggaacaaga agcagaagc taggagact tgacaagga 
      acgcaatat ctactacaa agagtacac caagcatca 
      aaagaatta tactcaagc ttgcgaaga tgctaagag 
      tttggagtg ccagttgaa ttacactca aatccaact 
      cgacaaact caagaaaac caacgtctt caacgcaac 
      gttccacat ctggcacaa aggccactt cggcaccat 
      caacaattt ctgccttgg aaggctgcc ttcggctcc 
      tgtcgactg gtctgaaat caacgcagc ttggggtca 
      aactgcact cttgctctc tgcacttgc acgcaaaat 
      aaatctgac ctttgaaag atacagatt agtgccata 
      cggcaatca ctcgtacat tgaggtaat tggcgaaca 
      aaaagagtt ccctttgta cggttcggg cggttttaa 
      gtttttgtg ggacactaa gtttgacaa cggaatggc 
      cgcgttttt agattgctt acaacagtt tcaggaaaa 
      ggtggagaa attggaaaa ggggaataa acagttttg 
      ttttccgta tcgaacaaa taaggggaa aattgagga 
      caatgacgc gagttactc aataaaaat tcagttaaa 
      ttctgagga acaatggac caaagcaat gaaatttat 
      gttaactaa tttgaagtg gggtctagc ttggatttc 
      atcacagtt aaataacga tatagaaga gaatatgca 
      aacggaaaa ttagtgtta ttgcatgtt tagtatttt 
      ttatattta agtaatttt agtacctac aagtaaatt 
      acgaaatct ataccatat ttttgttat gaaaatgac 
      tgatgttgt tgtaattat gttcaataa atttaaagt 
      agccaa