| General information | |||
| TC009813 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 8508805..8513962 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC009813 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01613 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Days post injection
beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
Days post injection
Pupal morphology
beetle stock: bad (increased lethality),
Analysis of embryonic musculature and early development
number of eggs/embryos/larvae on slide: > 50
embryo/egg not developed/not fertilized -
Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: > 50
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
- >TC009813 RA
MLRYGLIYI ATFMFVCGE YSAIKDQAM RSYDDKFSA EIFDNKQEP RENTDPSNQ KRPTCAQKN KNNGNKIDN LDIDEKELA KIGKLFMHH LAKDGRKRV DTNDVPMEE PDEEDHGQY VDQDFLDEM QKIENITNE GNKLYDELG GPPTIKYDP DPDNLKSIC CTEDESLID DSNTNHHHL KMHFYKFSP EKIDDKRAI SLYPDRNQF YKIPQKFNY IAFLSNDDK FMDVPAVKL NVFSDDLKR GKRTETFVP FFTTLYKRS AKNKLKFRP LRKRFTHTP VLFFNTISG AKRYRRLAK TTSKPAVSN VMKDADALL NKVNFFLKL GDEENEIRQ ISAHVFPKK QVKKEIKKN NGFKEWFED DYEEPRTKK ESQNQLGTS SLKENFNLL LDFDGELKT TEATTMRQL TTTACKKNA IDMSPIEIE NMMKDVVAK IDLPSLARN DFASRGYQN KLLNSEESG SSKEDLVND FIKDDNFKS EINPENNNL GETIVAEPP IFGNSLLYD INENKIQSD IENTTDAQT TEKPGNLSC AYLLDLFKN IEALKKTDP NLQKICPKL SELSHMGEN STTTEECLN KTSVVTNET TVKMEVTTP ETTSTFVCV TTETSSVSS SLTTQEITS PTSCTTEST IDTSSVPSS LATEEITSP TSSTTESTT IDTSPVSSS LTTQEITSA TSWTTESAI IDTSLASLI STTEEISSS LESSSTEST TQEDVSVSL TPYNMTHET PPKTSPFPT KIKEATSTI LEHSQSVPP QQSNELLTS FRFANKISR DLEGVSKEQ SSSTISTQK TTETPCLPT KIDETMTSE ENTSLPMEE NDLSESFQL LNKTFLQLE SAFENLTSG NAKTSGVQG GTAKEVRRT TTEVEKTSA NYEVSETTS EIATFSAVS EIIVPIIEV TETSSTSST PAETAELTL QTSSSPVAE VSSTAENTS EETTVLMSE KTTNFAFET TLETSRETE MTSSVISSS KLTPSTSST KEAYAEENL AQFTKPIPT SVLKMEGAT NSTFSLIAI PETSAKLEE TSPQTTSFN IATEKQMLE SKESPTVSS TIKEEFAHF TKPVPTSLL KQEGGTVTT YSSIATPET SASTETETI IMTTGSTTY SPPQLEETS PQTTSFNIA TENQMPESK ESPTVSSTT KEEYAEENF VHFTKPVPA SVLNMEGEK QMPESEESP TVSSTTKEE YAEENFEHF AKPVPTSVL KMEEITTNE YFSIANEVP IISATPIED VKLTSLAKI LFPPYIHIP TYKTDEVED NVPKEINPL VTFSTTELQ NGPTIYAPY AQIPTYRTN QFQEVSSPP FPPQFTPLP STSNSLTTS SQNLEDVSN EYAHYRDEE LSAILNLLG HDSSTVSTD NDTCGGMLG KIKRKVFTV KNLLLKSKN RQKEHGIRR KKDVHLRND LRPKRKRVP DSKEKRMKQ ETEVGEKSE EEEEMSIKM NNRCGF
- >TC009813 RB
MLRYGLIYI ATFMFVCGE YSAIKDQAM RSYDDKFSA EIFDNKQEP RENTDPSNQ KRPTCAQKN KNNGNKIDN LDIDEKELA KIGKLFMHH LAKDGRKRV DTNDVPMEE PDEEDHGQY VDQDFLDEM QKIENITNE GNKLYDELG GPPTIKYDP DPDNLKSIC CTEDESLID DSNTNHHHL KMHFYKFSP EKIDDKRAI SLYPDRNQF YKIPQKFNY IAFLSNDDK FMDVPAVKL NVFSDDLKR GKRTETFVP FFTTLYKRS AKNKLKFRP LRKRFTHTP VLFFNTISG AKRYRRLAK TTSKPAVSN VMKDADALL NKVNFFLKL GDEENEIRQ ISAHVFPKK QVKKEIKKN NGFKEWFED DYEEPRTKK ESQNQLGTS SLKENFNLL LDFDGELKT TEATTMRQL TTTACKKNA IDMSPIEIE NMMKDVVAK IDLPSLARN DFASRGYQN KLLNSEESG SSKEDLVND FIKDDNFKS EINPENNNL GETIVAEPP IFGNSLLYD INENKIQSD IENTTDAQT TEKPGNLSC AYLLDLFKN IEALKKTDP NLQKICPKL SELSHMGEN STTTEECLN KTSVVTNET TVKMEVTTP ETTSTFVCV TTETSSVSS SLTTQEITS PTSCTTEST IDTSSVPSS LATEEITSP TSSTTESTT IDTSPVSSS LTTQEITSA TSWTTESAI IDTSLASLI STTEEISSS LESSSTEST TQEDVSVSL TPYNMTHET PPKTSPFPT KIKEATSTI LEHSQSVPP QQSNELLTS FRFANKISR DLEGVSKEQ SSSTISTQK TTETPCLPT KIDETMTSE ENTSLPMEE NDLSESFQL LNKTFLQLE SAFENLTSG NAKTSGVQG GTAKEVRRT TTEVEKTSA NYEVSETTS EIATFSAVS EIIVPIIEV TETSSTSST PAETAELTL QTSSSPVAE VSSTAENTS EETTVLMSE KTTNFAFET TLETSRETE MTSSVISSS KLTPSTSST KEAYAEENL AQFTKPIPT SVLKMEGAT NSTFSLIAI PETSAKLEE TSPQTTSFN IATEKQMLE SKESPTVSS TIKEEFAHF TKPVPTSLL KQEGGTVTT YSSIATPET SASTETETI IMTTGSTTY SPPQLEETS PQTTSFNIA TENQMPESK ESPTVSSTT KEEYAEENF VHFTKPVPA SVLNMEGEK QMPESEESP TVSSTTKEE YAEENFEHF AKPVPTSVL KMEEITTNE YFSIANEVP IISATPIED VKLTSLAKI LFPPYIHIP TYKTDEVED NVPKEINPL VTFSTTELQ NGPTIYAPY AQIPTYRTN QFQEVSSPP FPPQFTPLP STSNSLTTS SQNLEDVSN EYAHYRDEE LSAILNLLG HDSSTVSTD NDTCGGMLG KIKRKVFSK LHTAQKELK LVLAVKNLL LKSKNRQKE HGIRRKKDV HLRNDLRPK RKRVPDSKE KRMKQETEV GEKSEEEEE MSIKMNNRC GF
- >TC009813 RA
atgcttcgc tatggttta atttacatt gccactttt atgtttgtt tgtggggaa tatagcgca ataaaagac caagcgatg cggtcatac gatgataaa ttctcagca gaaattttt gacaataag caagaacct agagaaaac actgatccc tcaaaccaa aaacgtcca acttgtgca caaaaaaac aaaaacaat gggaacaaa atagacaat ttagatata gacgaaaaa gaattggcg aaaatcggt aaactgttc atgcaccat ttggccaaa gatgggcgg aaaagggtt gataccaat gatgtaccg atggaagaa cctgacgaa gaagaccac gggcaatac gtcgatcaa gattttctt gacgaaatg caaaaaatt gagaatatt actaatgaa ggcaacaag ctttacgac gaactcgga ggtccccca acaatcaaa tatgaccca gatcccgac aatttgaaa agtatttgc tgcactgaa gacgaatca cttatcgat gattcaaac acaaatcat caccattta aaaatgcat ttttacaag tttagtccc gaaaaaatc gatgataag agagcgatt agtttatac cccgacagg aatcagttt tataagata ccccaaaaa tttaattac atcgctttt ctatcaaac gacgacaaa ttcatggat gttcctgcg gtcaaattg aatgttttt tcagacgat ttgaaacgg ggcaagcga accgaaact tttgttcct ttttttacc actctatac aagagaagc gccaaaaat aaattaaaa ttcaggccc ctacgaaaa cgcttcaca cacactcct gttctgttt ttcaacaca atttctggt gctaaaaga tacagacgc cttgctaaa acaacttca aaacccgcg gtttcgaat gttatgaaa gacgctgat gcattgcta aataaagta aactttttt ctaaagttg ggagacgaa gaaaacgaa atacgtcaa atttccgct catgttttt ccaaaaaaa caagttaaa aaagaaatt aaaaaaaac aatgggttt aaagaatgg ttcgaggat gattatgaa gagccacgg acaaaaaaa gagagccaa aatcagtta ggaacttct tcacttaaa gaaaatttc aatttactt ctggatttt gatggtgaa ttaaaaaca acagaagct acgacaatg agacaattg accacaacg gcgtgcaag aaaaatgca atagacatg tcacccatt gaaatcgaa aatatgatg aaggacgtt gttgcgaaa atagatctt ccttctttg gccagaaat gactttgcg agtcgggga tatcaaaac aaattgcta aattctgaa gaatcagga tcgtccaaa gaagatttg gtaaatgat tttattaaa gacgataat tttaaaagc gaaataaat ccagaaaat aataatcta ggcgaaacg atagtcgca gagccaccc attttcggt aattcttta ctgtatgat atcaacgaa aataaaatt caaagtgac atagaaaat acaaccgat gcacaaact acggaaaaa ccaggaaat ttatcgtgc gcttattta ctcgatctt tttaaaaat atcgaagca ttgaagaaa acagatcca aatttgcaa aaaatatgt cctaaatta agtgaactc tcacacatg ggtgaaaat tcaacaacc acagaagaa tgtctgaac aaaacgtcg gtagtaacg aatgaaaca actgtaaaa atggaagta acaacacca gaaactacg tcaactttc gtttgtgta actacagaa acttcttcg gtgtcgtcg tctttaaca acgcaagaa attacttca cctacttct tgtactaca gaatcaaca atagacact tcttcggtt ccgtcgtct ttagcaacg gaagaaatt acttcacct acttcttct actacagaa tcaacaaca atagacact tctccggtt tcctcatct ttaacaacg caagaaatt acttcagct acttcttgg actacagaa tcagcaata atagacact tctttggcc tcgttaatt tctaccaca gaagaaatt tcttcatct ttagaatct tcatctaca gaatcaacg acgcaagaa gatgtttca gtttcactt acaccatac aacatgacg catgaaact ccaccaaaa acttctcca tttccgacc aaaattaaa gaggcaact tcaacgata ctagaacac agtcagtcc gtaccgccc caacagagt aacgaacta ttaacatca tttcgtttc gcgaataaa atatcccgt gacttggaa ggagtatca aaagaacaa tcctcttca accataagc acacaaaaa actacagaa actccatgt cttccaaca aaaattgat gagacaatg acatcggaa gaaaatacg tctcttcca atggaggaa aatgatctt tcggaatcg tttcagctg ctcaataaa acgtttcta cagttagaa tcagccttt gaaaatctg acttctgga aatgcaaaa acaagtggt gttcaaggt ggtactgca aaagaagta cgcagaacc actacagag gtggaaaag acatcagca aattacgaa gtttcagaa actacctct gaaattgca actttttct gcagtttcc gagatcata gttccaata atagaagta acagaaact tcatcaacc tctagtact cctgcagaa actgcagaa ttaacacta caaacatca tcatctcct gtagctgaa gttagttct actgcagaa aatacttct gaagaaacg acagtcttg atgtctgaa aagaccaca aattttgcc tttgaaact acactcgaa actagtagg gaaactgaa atgacgagt tccgttatt tcatcttca aagttaacc ccaagtacg agcagtacc aaagaagca tatgctgaa gaaaatttg gcgcaattt acaaaacct attccaact agtgttttg aaaatggag ggggctaca aacagcact ttttcattg atagcaata ccagaaact agtgctaaa ttagaagaa acaagtccg caaacaaca tcattcaac attgcaaca gaaaaacaa atgcttgaa tcaaaagaa agcccaact gtgagcagt actatcaaa gaagaattt gcgcacttt acaaaacct gttccaaca agtcttttg aaacaggag gggggtaca gtcaccaca tattcatcg atagcaacg ccagaaact agtgctagt accgaaact gaaacaata attatgaca actggttct actacttat tcacctcca caattagaa gaaacaagt ccgcaaaca acatcattc aacattgct acagaaaac caaatgcct gaatccaaa gaaagccca actgtgagc agtactacc aaagaagaa tatgctgaa gaaaacttt gtgcacttc acaaaacct gttccagca agtgttttg aatatggag ggggaaaaa caaatgcct gaatccgag gaaagccca actgtgagc agtactacc aaagaagaa tatgctgaa gaaaacttt gagcacttc gcaaaacct gttccaaca agtgttttg aaaatggaa gagattaca accaacgaa tatttttca atcgcaaat gaagtgcct ataatttct gctactcct atagaagat gtgaaattg acttcattg gcaaagata cttttccct ccatatatc cacatccca acatataaa acagacgag gttgaagac aatgttcct aaagaaata aatccttta gtaactttt tcaactact gaacttcaa aacggtcca acaatatat gccccgtat gctcagatt ccaacctac agaacaaat caatttcaa gaagtatca tcaccacct tttcctcct cagtttact cctcttcct tccacatca aattcgcta accacgtcg tcacaaaat ttggaagat gtgtctaac gaatatgcc cactatcga gacgaagaa cttagtgct attttgaat ttactaggg cacgatagc agtaccgtc tccacggac aatgatact tgcggaggt atgcttgga aaaatcaaa aggaaagtt tttaccgta aaaaattta cttcttaaa tcgaaaaat cggcaaaag gagcatgga atcagaaga aaaaaagat gtacatctt cgtaatgat cttcgacct aaaaggaaa agagtacca gactctaag gaaaaaaga atgaaacaa gaaacagaa gtcggcgaa aaatcggaa gaagaggag gaaatgagc attaaaatg aacaataga tgtggattc taa
- >TC009813 RB
atgcttcgc tatggttta atttacatt gccactttt atgtttgtt tgtggggaa tatagcgca ataaaagac caagcgatg cggtcatac gatgataaa ttctcagca gaaattttt gacaataag caagaacct agagaaaac actgatccc tcaaaccaa aaacgtcca acttgtgca caaaaaaac aaaaacaat gggaacaaa atagacaat ttagatata gacgaaaaa gaattggcg aaaatcggt aaactgttc atgcaccat ttggccaaa gatgggcgg aaaagggtt gataccaat gatgtaccg atggaagaa cctgacgaa gaagaccac gggcaatac gtcgatcaa gattttctt gacgaaatg caaaaaatt gagaatatt actaatgaa ggcaacaag ctttacgac gaactcgga ggtccccca acaatcaaa tatgaccca gatcccgac aatttgaaa agtatttgc tgcactgaa gacgaatca cttatcgat gattcaaac acaaatcat caccattta aaaatgcat ttttacaag tttagtccc gaaaaaatc gatgataag agagcgatt agtttatac cccgacagg aatcagttt tataagata ccccaaaaa tttaattac atcgctttt ctatcaaac gacgacaaa ttcatggat gttcctgcg gtcaaattg aatgttttt tcagacgat ttgaaacgg ggcaagcga accgaaact tttgttcct ttttttacc actctatac aagagaagc gccaaaaat aaattaaaa ttcaggccc ctacgaaaa cgcttcaca cacactcct gttctgttt ttcaacaca atttctggt gctaaaaga tacagacgc cttgctaaa acaacttca aaacccgcg gtttcgaat gttatgaaa gacgctgat gcattgcta aataaagta aactttttt ctaaagttg ggagacgaa gaaaacgaa atacgtcaa atttccgct catgttttt ccaaaaaaa caagttaaa aaagaaatt aaaaaaaac aatgggttt aaagaatgg ttcgaggat gattatgaa gagccacgg acaaaaaaa gagagccaa aatcagtta ggaacttct tcacttaaa gaaaatttc aatttactt ctggatttt gatggtgaa ttaaaaaca acagaagct acgacaatg agacaattg accacaacg gcgtgcaag aaaaatgca atagacatg tcacccatt gaaatcgaa aatatgatg aaggacgtt gttgcgaaa atagatctt ccttctttg gccagaaat gactttgcg agtcgggga tatcaaaac aaattgcta aattctgaa gaatcagga tcgtccaaa gaagatttg gtaaatgat tttattaaa gacgataat tttaaaagc gaaataaat ccagaaaat aataatcta ggcgaaacg atagtcgca gagccaccc attttcggt aattcttta ctgtatgat atcaacgaa aataaaatt caaagtgac atagaaaat acaaccgat gcacaaact acggaaaaa ccaggaaat ttatcgtgc gcttattta ctcgatctt tttaaaaat atcgaagca ttgaagaaa acagatcca aatttgcaa aaaatatgt cctaaatta agtgaactc tcacacatg ggtgaaaat tcaacaacc acagaagaa tgtctgaac aaaacgtcg gtagtaacg aatgaaaca actgtaaaa atggaagta acaacacca gaaactacg tcaactttc gtttgtgta actacagaa acttcttcg gtgtcgtcg tctttaaca acgcaagaa attacttca cctacttct tgtactaca gaatcaaca atagacact tcttcggtt ccgtcgtct ttagcaacg gaagaaatt acttcacct acttcttct actacagaa tcaacaaca atagacact tctccggtt tcctcatct ttaacaacg caagaaatt acttcagct acttcttgg actacagaa tcagcaata atagacact tctttggcc tcgttaatt tctaccaca gaagaaatt tcttcatct ttagaatct tcatctaca gaatcaacg acgcaagaa gatgtttca gtttcactt acaccatac aacatgacg catgaaact ccaccaaaa acttctcca tttccgacc aaaattaaa gaggcaact tcaacgata ctagaacac agtcagtcc gtaccgccc caacagagt aacgaacta ttaacatca tttcgtttc gcgaataaa atatcccgt gacttggaa ggagtatca aaagaacaa tcctcttca accataagc acacaaaaa actacagaa actccatgt cttccaaca aaaattgat gagacaatg acatcggaa gaaaatacg tctcttcca atggaggaa aatgatctt tcggaatcg tttcagctg ctcaataaa acgtttcta cagttagaa tcagccttt gaaaatctg acttctgga aatgcaaaa acaagtggt gttcaaggt ggtactgca aaagaagta cgcagaacc actacagag gtggaaaag acatcagca aattacgaa gtttcagaa actacctct gaaattgca actttttct gcagtttcc gagatcata gttccaata atagaagta acagaaact tcatcaacc tctagtact cctgcagaa actgcagaa ttaacacta caaacatca tcatctcct gtagctgaa gttagttct actgcagaa aatacttct gaagaaacg acagtcttg atgtctgaa aagaccaca aattttgcc tttgaaact acactcgaa actagtagg gaaactgaa atgacgagt tccgttatt tcatcttca aagttaacc ccaagtacg agcagtacc aaagaagca tatgctgaa gaaaatttg gcgcaattt acaaaacct attccaact agtgttttg aaaatggag ggggctaca aacagcact ttttcattg atagcaata ccagaaact agtgctaaa ttagaagaa acaagtccg caaacaaca tcattcaac attgcaaca gaaaaacaa atgcttgaa tcaaaagaa agcccaact gtgagcagt actatcaaa gaagaattt gcgcacttt acaaaacct gttccaaca agtcttttg aaacaggag gggggtaca gtcaccaca tattcatcg atagcaacg ccagaaact agtgctagt accgaaact gaaacaata attatgaca actggttct actacttat tcacctcca caattagaa gaaacaagt ccgcaaaca acatcattc aacattgct acagaaaac caaatgcct gaatccaaa gaaagccca actgtgagc agtactacc aaagaagaa tatgctgaa gaaaacttt gtgcacttc acaaaacct gttccagca agtgttttg aatatggag ggggaaaaa caaatgcct gaatccgag gaaagccca actgtgagc agtactacc aaagaagaa tatgctgaa gaaaacttt gagcacttc gcaaaacct gttccaaca agtgttttg aaaatggaa gagattaca accaacgaa tatttttca atcgcaaat gaagtgcct ataatttct gctactcct atagaagat gtgaaattg acttcattg gcaaagata cttttccct ccatatatc cacatccca acatataaa acagacgag gttgaagac aatgttcct aaagaaata aatccttta gtaactttt tcaactact gaacttcaa aacggtcca acaatatat gccccgtat gctcagatt ccaacctac agaacaaat caatttcaa gaagtatca tcaccacct tttcctcct cagtttact cctcttcct tccacatca aattcgcta accacgtcg tcacaaaat ttggaagat gtgtctaac gaatatgcc cactatcga gacgaagaa cttagtgct attttgaat ttactaggg cacgatagc agtaccgtc tccacggac aatgatact tgcggaggt atgcttgga aaaatcaaa aggaaagtt tttagtaag ttgcacact gctcaaaaa gaattgaaa ctagtttta gccgtaaaa aatttactt cttaaatcg aaaaatcgg caaaaggag catggaatc agaagaaaa aaagatgta catcttcgt aatgatctt cgacctaaa aggaaaaga gtaccagac tctaaggaa aaaagaatg aaacaagaa acagaagtc ggcgaaaaa tcggaagaa gaggaggaa atgagcatt aaaatgaac aatagatgt ggattctaa
- >TC009813 RA
agccatatt ttcaaatgc ttcgctatg gtttaattt acattgcca cttttatgt ttgtttgtg gggaatata gcgcaataa aagaccaag cgatgcggt catacgatg ataaattct cagcagaaa tttttgaca ataagcaag aacctagag aaaacactg atccctcaa accaaaaac gtccaactt gtgcacaaa aaaacaaaa acaatggga acaaaatag acaatttag atatagacg aaaaagaat tggcgaaaa tcggtaaac tgttcatgc accatttgg ccaaagatg ggcggaaaa gggttgata ccaatgatg taccgatgg aagaacctg acgaagaag accacgggc aatacgtcg atcaagatt ttcttgacg aaatgcaaa aaattgaga atattacta atgaaggca acaagcttt acgacgaac tcggaggtc ccccaacaa tcaaatatg acccagatc ccgacaatt tgaaaagta tttgctgca ctgaagacg aatcactta tcgatgatt caaacacaa atcatcacc atttaaaaa tgcattttt acaagttta gtcccgaaa aaatcgatg ataagagag cgattagtt tataccccg acaggaatc agttttata agatacccc aaaaattta attacatcg cttttctat caaacgacg acaaattca tggatgttc ctgcggtca aattgaatg ttttttcag acgatttga aacggggca agcgaaccg aaacttttg ttccttttt ttaccactc tatacaaga gaagcgcca aaaataaat taaaattca ggcccctac gaaaacgct tcacacaca ctcctgttc tgtttttca acacaattt ctggtgcta aaagataca gacgccttg ctaaaacaa cttcaaaac ccgcggttt cgaatgtta tgaaagacg ctgatgcat tgctaaata aagtaaact tttttctaa agttgggag acgaagaaa acgaaatac gtcaaattt ccgctcatg tttttccaa aaaaacaag ttaaaaaag aaattaaaa aaaacaatg ggtttaaag aatggttcg aggatgatt atgaagagc cacggacaa aaaaagaga gccaaaatc agttaggaa cttcttcac ttaaagaaa atttcaatt tacttctgg attttgatg gtgaattaa aaacaacag aagctacga caatgagac aattgacca caacggcgt gcaagaaaa atgcaatag acatgtcac ccattgaaa tcgaaaata tgatgaagg acgttgttg cgaaaatag atcttcctt ctttggcca gaaatgact ttgcgagtc ggggatatc aaaacaaat tgctaaatt ctgaagaat caggatcgt ccaaagaag atttggtaa atgatttta ttaaagacg ataatttta aaagcgaaa taaatccag aaaataata atctaggcg aaacgatag tcgcagagc cacccattt tcggtaatt ctttactgt atgatatca acgaaaata aaattcaaa gtgacatag aaaatacaa ccgatgcac aaactacgg aaaaaccag gaaatttat cgtgcgctt atttactcg atcttttta aaaatatcg aagcattga agaaaacag atccaaatt tgcaaaaaa tatgtccta aattaagtg aactctcac acatgggtg aaaattcaa caaccacag aagaatgtc tgaacaaaa cgtcggtag taacgaatg aaacaactg taaaaatgg aagtaacaa caccagaaa ctacgtcaa ctttcgttt gtgtaacta cagaaactt cttcggtgt cgtcgtctt taacaacgc aagaaatta cttcaccta cttcttgta ctacagaat caacaatag acacttctt cggttccgt cgtctttag caacggaag aaattactt cacctactt cttctacta cagaatcaa caacaatag acacttctc cggtttcct catctttaa caacgcaag aaattactt cagctactt cttggacta cagaatcag caataatag acacttctt tggcctcgt taatttcta ccacagaag aaatttctt catctttag aatcttcat ctacagaat caacgacgc aagaagatg tttcagttt cacttacac catacaaca tgacgcatg aaactccac caaaaactt ctccatttc cgaccaaaa ttaaagagg caacttcaa cgatactag aacacagtc agtccgtac cgccccaac agagtaacg aactattaa catcatttc gtttcgcga ataaaatat cccgtgact tggaaggag tatcaaaag aacaatcct cttcaacca taagcacac aaaaaacta cagaaactc catgtcttc caacaaaaa ttgatgaga caatgacat cggaagaaa atacgtctc ttccaatgg aggaaaatg atctttcgg aatcgtttc agctgctca ataaaacgt ttctacagt tagaatcag cctttgaaa atctgactt ctggaaatg caaaaacaa gtggtgttc aaggtggta ctgcaaaag aagtacgca gaaccacta cagaggtgg aaaagacat cagcaaatt acgaagttt cagaaacta cctctgaaa ttgcaactt tttctgcag tttccgaga tcatagttc caataatag aagtaacag aaacttcat caacctcta gtactcctg cagaaactg cagaattaa cactacaaa catcatcat ctcctgtag ctgaagtta gttctactg cagaaaata cttctgaag aaacgacag tcttgatgt ctgaaaaga ccacaaatt ttgcctttg aaactacac tcgaaacta gtagggaaa ctgaaatga cgagttccg ttatttcat cttcaaagt taaccccaa gtacgagca gtaccaaag aagcatatg ctgaagaaa atttggcgc aatttacaa aacctattc caactagtg ttttgaaaa tggaggggg ctacaaaca gcacttttt cattgatag caataccag aaactagtg ctaaattag aagaaacaa gtccgcaaa caacatcat tcaacattg caacagaaa aacaaatgc ttgaatcaa aagaaagcc caactgtga gcagtacta tcaaagaag aatttgcgc actttacaa aacctgttc caacaagtc ttttgaaac aggaggggg gtacagtca ccacatatt catcgatag caacgccag aaactagtg ctagtaccg aaactgaaa caataatta tgacaactg gttctacta cttattcac ctccacaat tagaagaaa caagtccgc aaacaacat cattcaaca ttgctacag aaaaccaaa tgcctgaat ccaaagaaa gcccaactg tgagcagta ctaccaaag aagaatatg ctgaagaaa actttgtgc acttcacaa aacctgttc cagcaagtg ttttgaata tggaggggg aaaaacaaa tgcctgaat ccgaggaaa gcccaactg tgagcagta ctaccaaag aagaatatg ctgaagaaa actttgagc acttcgcaa aacctgttc caacaagtg ttttgaaaa tggaagaga ttacaacca acgaatatt tttcaatcg caaatgaag tgcctataa tttctgcta ctcctatag aagatgtga aattgactt cattggcaa agatacttt tccctccat atatccaca tcccaacat ataaaacag acgaggttg aagacaatg ttcctaaag aaataaatc ctttagtaa ctttttcaa ctactgaac ttcaaaacg gtccaacaa tatatgccc cgtatgctc agattccaa cctacagaa caaatcaat ttcaagaag tatcatcac caccttttc ctcctcagt ttactcctc ttccttcca catcaaatt cgctaacca cgtcgtcac aaaatttgg aagatgtgt ctaacgaat atgcccact atcgagacg aagaactta gtgctattt tgaatttac tagggcacg atagcagta ccgtctcca cggacaatg atacttgcg gaggtatgc ttggaaaaa tcaaaagga aagttttta ccgtaaaaa atttacttc ttaaatcga aaaatcggc aaaaggagc atggaatca gaagaaaaa aagatgtac atcttcgta atgatcttc gacctaaaa ggaaaagag taccagact ctaaggaaa aaagaatga aacaagaaa cagaagtcg gcgaaaaat cggaagaag aggaggaaa tgagcatta aaatgaaca atagatgtg gattctaaa cacaaagga gttcaaagg aagaccata caacaaagt gtctaatca cttaatttt aagtcataa caattgaaa atatgatga taggcgtga aaatgctat attaaatac agattttaa ttgagctgg gttttgtat tgtttgtaa ctttataag aaaacatat ggttgtggc actgtcacc tttactttt tgaatttgg tgtaacaaa atcttcagt cgaaatttt aaacatgaa gctgcttta aattattat ttggagact aacttttat tttaataaa gaatcaaat cagtt
- >TC009813 RB
tttttgtgt aagccatat tttcaaatg cttcgctat ggtttaatt tacattgcc acttttatg tttgtttgt ggggaatat agcgcaata aaagaccaa gcgatgcgg tcatacgat gataaattc tcagcagaa atttttgac aataagcaa gaacctaga gaaaacact gatccctca aaccaaaaa cgtccaact tgtgcacaa aaaaacaaa aacaatggg aacaaaata gacaattta gatatagac gaaaaagaa ttggcgaaa atcggtaaa ctgttcatg caccatttg gccaaagat gggcggaaa agggttgat accaatgat gtaccgatg gaagaacct gacgaagaa gaccacggg caatacgtc gatcaagat tttcttgac gaaatgcaa aaaattgag aatattact aatgaaggc aacaagctt tacgacgaa ctcggaggt cccccaaca atcaaatat gacccagat cccgacaat ttgaaaagt atttgctgc actgaagac gaatcactt atcgatgat tcaaacaca aatcatcac catttaaaa atgcatttt tacaagttt agtcccgaa aaaatcgat gataagaga gcgattagt ttatacccc gacaggaat cagttttat aagataccc caaaaattt aattacatc gcttttcta tcaaacgac gacaaattc atggatgtt cctgcggtc aaattgaat gttttttca gacgatttg aaacggggc aagcgaacc gaaactttt gttcctttt tttaccact ctatacaag agaagcgcc aaaaataaa ttaaaattc aggccccta cgaaaacgc ttcacacac actcctgtt ctgtttttc aacacaatt tctggtgct aaaagatac agacgcctt gctaaaaca acttcaaaa cccgcggtt tcgaatgtt atgaaagac gctgatgca ttgctaaat aaagtaaac ttttttcta aagttggga gacgaagaa aacgaaata cgtcaaatt tccgctcat gtttttcca aaaaaacaa gttaaaaaa gaaattaaa aaaaacaat gggtttaaa gaatggttc gaggatgat tatgaagag ccacggaca aaaaaagag agccaaaat cagttagga acttcttca cttaaagaa aatttcaat ttacttctg gattttgat ggtgaatta aaaacaaca gaagctacg acaatgaga caattgacc acaacggcg tgcaagaaa aatgcaata gacatgtca cccattgaa atcgaaaat atgatgaag gacgttgtt gcgaaaata gatcttcct tctttggcc agaaatgac tttgcgagt cggggatat caaaacaaa ttgctaaat tctgaagaa tcaggatcg tccaaagaa gatttggta aatgatttt attaaagac gataatttt aaaagcgaa ataaatcca gaaaataat aatctaggc gaaacgata gtcgcagag ccacccatt ttcggtaat tctttactg tatgatatc aacgaaaat aaaattcaa agtgacata gaaaataca accgatgca caaactacg gaaaaacca ggaaattta tcgtgcgct tatttactc gatcttttt aaaaatatc gaagcattg aagaaaaca gatccaaat ttgcaaaaa atatgtcct aaattaagt gaactctca cacatgggt gaaaattca acaaccaca gaagaatgt ctgaacaaa acgtcggta gtaacgaat gaaacaact gtaaaaatg gaagtaaca acaccagaa actacgtca actttcgtt tgtgtaact acagaaact tcttcggtg tcgtcgtct ttaacaacg caagaaatt acttcacct acttcttgt actacagaa tcaacaata gacacttct tcggttccg tcgtcttta gcaacggaa gaaattact tcacctact tcttctact acagaatca acaacaata gacacttct ccggtttcc tcatcttta acaacgcaa gaaattact tcagctact tcttggact acagaatca gcaataata gacacttct ttggcctcg ttaatttct accacagaa gaaatttct tcatcttta gaatcttca tctacagaa tcaacgacg caagaagat gtttcagtt tcacttaca ccatacaac atgacgcat gaaactcca ccaaaaact tctccattt ccgaccaaa attaaagag gcaacttca acgatacta gaacacagt cagtccgta ccgccccaa cagagtaac gaactatta acatcattt cgtttcgcg aataaaata tcccgtgac ttggaagga gtatcaaaa gaacaatcc tcttcaacc ataagcaca caaaaaact acagaaact ccatgtctt ccaacaaaa attgatgag acaatgaca tcggaagaa aatacgtct cttccaatg gaggaaaat gatctttcg gaatcgttt cagctgctc aataaaacg tttctacag ttagaatca gcctttgaa aatctgact tctggaaat gcaaaaaca agtggtgtt caaggtggt actgcaaaa gaagtacgc agaaccact acagaggtg gaaaagaca tcagcaaat tacgaagtt tcagaaact acctctgaa attgcaact ttttctgca gtttccgag atcatagtt ccaataata gaagtaaca gaaacttca tcaacctct agtactcct gcagaaact gcagaatta acactacaa acatcatca tctcctgta gctgaagtt agttctact gcagaaaat acttctgaa gaaacgaca gtcttgatg tctgaaaag accacaaat tttgccttt gaaactaca ctcgaaact agtagggaa actgaaatg acgagttcc gttatttca tcttcaaag ttaacccca agtacgagc agtaccaaa gaagcatat gctgaagaa aatttggcg caatttaca aaacctatt ccaactagt gttttgaaa atggagggg gctacaaac agcactttt tcattgata gcaatacca gaaactagt gctaaatta gaagaaaca agtccgcaa acaacatca ttcaacatt gcaacagaa aaacaaatg cttgaatca aaagaaagc ccaactgtg agcagtact atcaaagaa gaatttgcg cactttaca aaacctgtt ccaacaagt cttttgaaa caggagggg ggtacagtc accacatat tcatcgata gcaacgcca gaaactagt gctagtacc gaaactgaa acaataatt atgacaact ggttctact acttattca cctccacaa ttagaagaa acaagtccg caaacaaca tcattcaac attgctaca gaaaaccaa atgcctgaa tccaaagaa agcccaact gtgagcagt actaccaaa gaagaatat gctgaagaa aactttgtg cacttcaca aaacctgtt ccagcaagt gttttgaat atggagggg gaaaaacaa atgcctgaa tccgaggaa agcccaact gtgagcagt actaccaaa gaagaatat gctgaagaa aactttgag cacttcgca aaacctgtt ccaacaagt gttttgaaa atggaagag attacaacc aacgaatat ttttcaatc gcaaatgaa gtgcctata atttctgct actcctata gaagatgtg aaattgact tcattggca aagatactt ttccctcca tatatccac atcccaaca tataaaaca gacgaggtt gaagacaat gttcctaaa gaaataaat cctttagta actttttca actactgaa cttcaaaac ggtccaaca atatatgcc ccgtatgct cagattcca acctacaga acaaatcaa tttcaagaa gtatcatca ccacctttt cctcctcag tttactcct cttccttcc acatcaaat tcgctaacc acgtcgtca caaaatttg gaagatgtg tctaacgaa tatgcccac tatcgagac gaagaactt agtgctatt ttgaattta ctagggcac gatagcagt accgtctcc acggacaat gatacttgc ggaggtatg cttggaaaa atcaaaagg aaagttttt agtaagttg cacactgct caaaaagaa ttgaaacta gttttagcc gtaaaaaat ttacttctt aaatcgaaa aatcggcaa aaggagcat ggaatcaga agaaaaaaa gatgtacat cttcgtaat gatcttcga cctaaaagg aaaagagta ccagactct aaggaaaaa agaatgaaa caagaaaca gaagtcggc gaaaaatcg gaagaagag gaggaaatg agcattaaa atgaacaat agatgtgga ttctaaaca caaaggagt tcaaaggaa gaccataca acaaagtgt ctaatcact taattttaa gtcataaca attgaaaat atgatgata ggcgtgaaa atgctatat taaatacag attttaatt gagctgggt tttgtattg tttgtaact ttataagaa aacatatgg ttgtgg
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