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Gene information
General information
TC009813 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8508805..8513962
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC009813
      iBeetle screen iB_01613

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      Pupal morphology
      beetle stock: bad (increased lethality),
      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC009813 RA
        MLRYGLIYI ATFMFVCGE YSAIKDQAM RSYDDKFSA 
        EIFDNKQEP RENTDPSNQ KRPTCAQKN KNNGNKIDN 
        LDIDEKELA KIGKLFMHH LAKDGRKRV DTNDVPMEE 
        PDEEDHGQY VDQDFLDEM QKIENITNE GNKLYDELG 
        GPPTIKYDP DPDNLKSIC CTEDESLID DSNTNHHHL 
        KMHFYKFSP EKIDDKRAI SLYPDRNQF YKIPQKFNY 
        IAFLSNDDK FMDVPAVKL NVFSDDLKR GKRTETFVP 
        FFTTLYKRS AKNKLKFRP LRKRFTHTP VLFFNTISG 
        AKRYRRLAK TTSKPAVSN VMKDADALL NKVNFFLKL 
        GDEENEIRQ ISAHVFPKK QVKKEIKKN NGFKEWFED 
        DYEEPRTKK ESQNQLGTS SLKENFNLL LDFDGELKT 
        TEATTMRQL TTTACKKNA IDMSPIEIE NMMKDVVAK 
        IDLPSLARN DFASRGYQN KLLNSEESG SSKEDLVND 
        FIKDDNFKS EINPENNNL GETIVAEPP IFGNSLLYD 
        INENKIQSD IENTTDAQT TEKPGNLSC AYLLDLFKN 
        IEALKKTDP NLQKICPKL SELSHMGEN STTTEECLN 
        KTSVVTNET TVKMEVTTP ETTSTFVCV TTETSSVSS 
        SLTTQEITS PTSCTTEST IDTSSVPSS LATEEITSP 
        TSSTTESTT IDTSPVSSS LTTQEITSA TSWTTESAI 
        IDTSLASLI STTEEISSS LESSSTEST TQEDVSVSL 
        TPYNMTHET PPKTSPFPT KIKEATSTI LEHSQSVPP 
        QQSNELLTS FRFANKISR DLEGVSKEQ SSSTISTQK 
        TTETPCLPT KIDETMTSE ENTSLPMEE NDLSESFQL 
        LNKTFLQLE SAFENLTSG NAKTSGVQG GTAKEVRRT 
        TTEVEKTSA NYEVSETTS EIATFSAVS EIIVPIIEV 
        TETSSTSST PAETAELTL QTSSSPVAE VSSTAENTS 
        EETTVLMSE KTTNFAFET TLETSRETE MTSSVISSS 
        KLTPSTSST KEAYAEENL AQFTKPIPT SVLKMEGAT 
        NSTFSLIAI PETSAKLEE TSPQTTSFN IATEKQMLE 
        SKESPTVSS TIKEEFAHF TKPVPTSLL KQEGGTVTT 
        YSSIATPET SASTETETI IMTTGSTTY SPPQLEETS 
        PQTTSFNIA TENQMPESK ESPTVSSTT KEEYAEENF 
        VHFTKPVPA SVLNMEGEK QMPESEESP TVSSTTKEE 
        YAEENFEHF AKPVPTSVL KMEEITTNE YFSIANEVP 
        IISATPIED VKLTSLAKI LFPPYIHIP TYKTDEVED 
        NVPKEINPL VTFSTTELQ NGPTIYAPY AQIPTYRTN 
        QFQEVSSPP FPPQFTPLP STSNSLTTS SQNLEDVSN 
        EYAHYRDEE LSAILNLLG HDSSTVSTD NDTCGGMLG 
        KIKRKVFTV KNLLLKSKN RQKEHGIRR KKDVHLRND 
        LRPKRKRVP DSKEKRMKQ ETEVGEKSE EEEEMSIKM 
        NNRCGF
      • >TC009813 RB
        MLRYGLIYI ATFMFVCGE YSAIKDQAM RSYDDKFSA 
        EIFDNKQEP RENTDPSNQ KRPTCAQKN KNNGNKIDN 
        LDIDEKELA KIGKLFMHH LAKDGRKRV DTNDVPMEE 
        PDEEDHGQY VDQDFLDEM QKIENITNE GNKLYDELG 
        GPPTIKYDP DPDNLKSIC CTEDESLID DSNTNHHHL 
        KMHFYKFSP EKIDDKRAI SLYPDRNQF YKIPQKFNY 
        IAFLSNDDK FMDVPAVKL NVFSDDLKR GKRTETFVP 
        FFTTLYKRS AKNKLKFRP LRKRFTHTP VLFFNTISG 
        AKRYRRLAK TTSKPAVSN VMKDADALL NKVNFFLKL 
        GDEENEIRQ ISAHVFPKK QVKKEIKKN NGFKEWFED 
        DYEEPRTKK ESQNQLGTS SLKENFNLL LDFDGELKT 
        TEATTMRQL TTTACKKNA IDMSPIEIE NMMKDVVAK 
        IDLPSLARN DFASRGYQN KLLNSEESG SSKEDLVND 
        FIKDDNFKS EINPENNNL GETIVAEPP IFGNSLLYD 
        INENKIQSD IENTTDAQT TEKPGNLSC AYLLDLFKN 
        IEALKKTDP NLQKICPKL SELSHMGEN STTTEECLN 
        KTSVVTNET TVKMEVTTP ETTSTFVCV TTETSSVSS 
        SLTTQEITS PTSCTTEST IDTSSVPSS LATEEITSP 
        TSSTTESTT IDTSPVSSS LTTQEITSA TSWTTESAI 
        IDTSLASLI STTEEISSS LESSSTEST TQEDVSVSL 
        TPYNMTHET PPKTSPFPT KIKEATSTI LEHSQSVPP 
        QQSNELLTS FRFANKISR DLEGVSKEQ SSSTISTQK 
        TTETPCLPT KIDETMTSE ENTSLPMEE NDLSESFQL 
        LNKTFLQLE SAFENLTSG NAKTSGVQG GTAKEVRRT 
        TTEVEKTSA NYEVSETTS EIATFSAVS EIIVPIIEV 
        TETSSTSST PAETAELTL QTSSSPVAE VSSTAENTS 
        EETTVLMSE KTTNFAFET TLETSRETE MTSSVISSS 
        KLTPSTSST KEAYAEENL AQFTKPIPT SVLKMEGAT 
        NSTFSLIAI PETSAKLEE TSPQTTSFN IATEKQMLE 
        SKESPTVSS TIKEEFAHF TKPVPTSLL KQEGGTVTT 
        YSSIATPET SASTETETI IMTTGSTTY SPPQLEETS 
        PQTTSFNIA TENQMPESK ESPTVSSTT KEEYAEENF 
        VHFTKPVPA SVLNMEGEK QMPESEESP TVSSTTKEE 
        YAEENFEHF AKPVPTSVL KMEEITTNE YFSIANEVP 
        IISATPIED VKLTSLAKI LFPPYIHIP TYKTDEVED 
        NVPKEINPL VTFSTTELQ NGPTIYAPY AQIPTYRTN 
        QFQEVSSPP FPPQFTPLP STSNSLTTS SQNLEDVSN 
        EYAHYRDEE LSAILNLLG HDSSTVSTD NDTCGGMLG 
        KIKRKVFSK LHTAQKELK LVLAVKNLL LKSKNRQKE 
        HGIRRKKDV HLRNDLRPK RKRVPDSKE KRMKQETEV 
        GEKSEEEEE MSIKMNNRC GF
      CDS sequence
      • >TC009813 RA
        atgcttcgc tatggttta atttacatt gccactttt 
        atgtttgtt tgtggggaa tatagcgca ataaaagac 
        caagcgatg cggtcatac gatgataaa ttctcagca 
        gaaattttt gacaataag caagaacct agagaaaac 
        actgatccc tcaaaccaa aaacgtcca acttgtgca 
        caaaaaaac aaaaacaat gggaacaaa atagacaat 
        ttagatata gacgaaaaa gaattggcg aaaatcggt 
        aaactgttc atgcaccat ttggccaaa gatgggcgg 
        aaaagggtt gataccaat gatgtaccg atggaagaa 
        cctgacgaa gaagaccac gggcaatac gtcgatcaa 
        gattttctt gacgaaatg caaaaaatt gagaatatt 
        actaatgaa ggcaacaag ctttacgac gaactcgga 
        ggtccccca acaatcaaa tatgaccca gatcccgac 
        aatttgaaa agtatttgc tgcactgaa gacgaatca 
        cttatcgat gattcaaac acaaatcat caccattta 
        aaaatgcat ttttacaag tttagtccc gaaaaaatc 
        gatgataag agagcgatt agtttatac cccgacagg 
        aatcagttt tataagata ccccaaaaa tttaattac 
        atcgctttt ctatcaaac gacgacaaa ttcatggat 
        gttcctgcg gtcaaattg aatgttttt tcagacgat 
        ttgaaacgg ggcaagcga accgaaact tttgttcct 
        ttttttacc actctatac aagagaagc gccaaaaat 
        aaattaaaa ttcaggccc ctacgaaaa cgcttcaca 
        cacactcct gttctgttt ttcaacaca atttctggt 
        gctaaaaga tacagacgc cttgctaaa acaacttca 
        aaacccgcg gtttcgaat gttatgaaa gacgctgat 
        gcattgcta aataaagta aactttttt ctaaagttg 
        ggagacgaa gaaaacgaa atacgtcaa atttccgct 
        catgttttt ccaaaaaaa caagttaaa aaagaaatt 
        aaaaaaaac aatgggttt aaagaatgg ttcgaggat 
        gattatgaa gagccacgg acaaaaaaa gagagccaa 
        aatcagtta ggaacttct tcacttaaa gaaaatttc 
        aatttactt ctggatttt gatggtgaa ttaaaaaca 
        acagaagct acgacaatg agacaattg accacaacg 
        gcgtgcaag aaaaatgca atagacatg tcacccatt 
        gaaatcgaa aatatgatg aaggacgtt gttgcgaaa 
        atagatctt ccttctttg gccagaaat gactttgcg 
        agtcgggga tatcaaaac aaattgcta aattctgaa 
        gaatcagga tcgtccaaa gaagatttg gtaaatgat 
        tttattaaa gacgataat tttaaaagc gaaataaat 
        ccagaaaat aataatcta ggcgaaacg atagtcgca 
        gagccaccc attttcggt aattcttta ctgtatgat 
        atcaacgaa aataaaatt caaagtgac atagaaaat 
        acaaccgat gcacaaact acggaaaaa ccaggaaat 
        ttatcgtgc gcttattta ctcgatctt tttaaaaat 
        atcgaagca ttgaagaaa acagatcca aatttgcaa 
        aaaatatgt cctaaatta agtgaactc tcacacatg 
        ggtgaaaat tcaacaacc acagaagaa tgtctgaac 
        aaaacgtcg gtagtaacg aatgaaaca actgtaaaa 
        atggaagta acaacacca gaaactacg tcaactttc 
        gtttgtgta actacagaa acttcttcg gtgtcgtcg 
        tctttaaca acgcaagaa attacttca cctacttct 
        tgtactaca gaatcaaca atagacact tcttcggtt 
        ccgtcgtct ttagcaacg gaagaaatt acttcacct 
        acttcttct actacagaa tcaacaaca atagacact 
        tctccggtt tcctcatct ttaacaacg caagaaatt 
        acttcagct acttcttgg actacagaa tcagcaata 
        atagacact tctttggcc tcgttaatt tctaccaca 
        gaagaaatt tcttcatct ttagaatct tcatctaca 
        gaatcaacg acgcaagaa gatgtttca gtttcactt 
        acaccatac aacatgacg catgaaact ccaccaaaa 
        acttctcca tttccgacc aaaattaaa gaggcaact 
        tcaacgata ctagaacac agtcagtcc gtaccgccc 
        caacagagt aacgaacta ttaacatca tttcgtttc 
        gcgaataaa atatcccgt gacttggaa ggagtatca 
        aaagaacaa tcctcttca accataagc acacaaaaa 
        actacagaa actccatgt cttccaaca aaaattgat 
        gagacaatg acatcggaa gaaaatacg tctcttcca 
        atggaggaa aatgatctt tcggaatcg tttcagctg 
        ctcaataaa acgtttcta cagttagaa tcagccttt 
        gaaaatctg acttctgga aatgcaaaa acaagtggt 
        gttcaaggt ggtactgca aaagaagta cgcagaacc 
        actacagag gtggaaaag acatcagca aattacgaa 
        gtttcagaa actacctct gaaattgca actttttct 
        gcagtttcc gagatcata gttccaata atagaagta 
        acagaaact tcatcaacc tctagtact cctgcagaa 
        actgcagaa ttaacacta caaacatca tcatctcct 
        gtagctgaa gttagttct actgcagaa aatacttct 
        gaagaaacg acagtcttg atgtctgaa aagaccaca 
        aattttgcc tttgaaact acactcgaa actagtagg 
        gaaactgaa atgacgagt tccgttatt tcatcttca 
        aagttaacc ccaagtacg agcagtacc aaagaagca 
        tatgctgaa gaaaatttg gcgcaattt acaaaacct 
        attccaact agtgttttg aaaatggag ggggctaca 
        aacagcact ttttcattg atagcaata ccagaaact 
        agtgctaaa ttagaagaa acaagtccg caaacaaca 
        tcattcaac attgcaaca gaaaaacaa atgcttgaa 
        tcaaaagaa agcccaact gtgagcagt actatcaaa 
        gaagaattt gcgcacttt acaaaacct gttccaaca 
        agtcttttg aaacaggag gggggtaca gtcaccaca 
        tattcatcg atagcaacg ccagaaact agtgctagt 
        accgaaact gaaacaata attatgaca actggttct 
        actacttat tcacctcca caattagaa gaaacaagt 
        ccgcaaaca acatcattc aacattgct acagaaaac 
        caaatgcct gaatccaaa gaaagccca actgtgagc 
        agtactacc aaagaagaa tatgctgaa gaaaacttt 
        gtgcacttc acaaaacct gttccagca agtgttttg 
        aatatggag ggggaaaaa caaatgcct gaatccgag 
        gaaagccca actgtgagc agtactacc aaagaagaa 
        tatgctgaa gaaaacttt gagcacttc gcaaaacct 
        gttccaaca agtgttttg aaaatggaa gagattaca 
        accaacgaa tatttttca atcgcaaat gaagtgcct 
        ataatttct gctactcct atagaagat gtgaaattg 
        acttcattg gcaaagata cttttccct ccatatatc 
        cacatccca acatataaa acagacgag gttgaagac 
        aatgttcct aaagaaata aatccttta gtaactttt 
        tcaactact gaacttcaa aacggtcca acaatatat 
        gccccgtat gctcagatt ccaacctac agaacaaat 
        caatttcaa gaagtatca tcaccacct tttcctcct 
        cagtttact cctcttcct tccacatca aattcgcta 
        accacgtcg tcacaaaat ttggaagat gtgtctaac 
        gaatatgcc cactatcga gacgaagaa cttagtgct 
        attttgaat ttactaggg cacgatagc agtaccgtc 
        tccacggac aatgatact tgcggaggt atgcttgga 
        aaaatcaaa aggaaagtt tttaccgta aaaaattta 
        cttcttaaa tcgaaaaat cggcaaaag gagcatgga 
        atcagaaga aaaaaagat gtacatctt cgtaatgat 
        cttcgacct aaaaggaaa agagtacca gactctaag 
        gaaaaaaga atgaaacaa gaaacagaa gtcggcgaa 
        aaatcggaa gaagaggag gaaatgagc attaaaatg 
        aacaataga tgtggattc taa
      • >TC009813 RB
        atgcttcgc tatggttta atttacatt gccactttt 
        atgtttgtt tgtggggaa tatagcgca ataaaagac 
        caagcgatg cggtcatac gatgataaa ttctcagca 
        gaaattttt gacaataag caagaacct agagaaaac 
        actgatccc tcaaaccaa aaacgtcca acttgtgca 
        caaaaaaac aaaaacaat gggaacaaa atagacaat 
        ttagatata gacgaaaaa gaattggcg aaaatcggt 
        aaactgttc atgcaccat ttggccaaa gatgggcgg 
        aaaagggtt gataccaat gatgtaccg atggaagaa 
        cctgacgaa gaagaccac gggcaatac gtcgatcaa 
        gattttctt gacgaaatg caaaaaatt gagaatatt 
        actaatgaa ggcaacaag ctttacgac gaactcgga 
        ggtccccca acaatcaaa tatgaccca gatcccgac 
        aatttgaaa agtatttgc tgcactgaa gacgaatca 
        cttatcgat gattcaaac acaaatcat caccattta 
        aaaatgcat ttttacaag tttagtccc gaaaaaatc 
        gatgataag agagcgatt agtttatac cccgacagg 
        aatcagttt tataagata ccccaaaaa tttaattac 
        atcgctttt ctatcaaac gacgacaaa ttcatggat 
        gttcctgcg gtcaaattg aatgttttt tcagacgat 
        ttgaaacgg ggcaagcga accgaaact tttgttcct 
        ttttttacc actctatac aagagaagc gccaaaaat 
        aaattaaaa ttcaggccc ctacgaaaa cgcttcaca 
        cacactcct gttctgttt ttcaacaca atttctggt 
        gctaaaaga tacagacgc cttgctaaa acaacttca 
        aaacccgcg gtttcgaat gttatgaaa gacgctgat 
        gcattgcta aataaagta aactttttt ctaaagttg 
        ggagacgaa gaaaacgaa atacgtcaa atttccgct 
        catgttttt ccaaaaaaa caagttaaa aaagaaatt 
        aaaaaaaac aatgggttt aaagaatgg ttcgaggat 
        gattatgaa gagccacgg acaaaaaaa gagagccaa 
        aatcagtta ggaacttct tcacttaaa gaaaatttc 
        aatttactt ctggatttt gatggtgaa ttaaaaaca 
        acagaagct acgacaatg agacaattg accacaacg 
        gcgtgcaag aaaaatgca atagacatg tcacccatt 
        gaaatcgaa aatatgatg aaggacgtt gttgcgaaa 
        atagatctt ccttctttg gccagaaat gactttgcg 
        agtcgggga tatcaaaac aaattgcta aattctgaa 
        gaatcagga tcgtccaaa gaagatttg gtaaatgat 
        tttattaaa gacgataat tttaaaagc gaaataaat 
        ccagaaaat aataatcta ggcgaaacg atagtcgca 
        gagccaccc attttcggt aattcttta ctgtatgat 
        atcaacgaa aataaaatt caaagtgac atagaaaat 
        acaaccgat gcacaaact acggaaaaa ccaggaaat 
        ttatcgtgc gcttattta ctcgatctt tttaaaaat 
        atcgaagca ttgaagaaa acagatcca aatttgcaa 
        aaaatatgt cctaaatta agtgaactc tcacacatg 
        ggtgaaaat tcaacaacc acagaagaa tgtctgaac 
        aaaacgtcg gtagtaacg aatgaaaca actgtaaaa 
        atggaagta acaacacca gaaactacg tcaactttc 
        gtttgtgta actacagaa acttcttcg gtgtcgtcg 
        tctttaaca acgcaagaa attacttca cctacttct 
        tgtactaca gaatcaaca atagacact tcttcggtt 
        ccgtcgtct ttagcaacg gaagaaatt acttcacct 
        acttcttct actacagaa tcaacaaca atagacact 
        tctccggtt tcctcatct ttaacaacg caagaaatt 
        acttcagct acttcttgg actacagaa tcagcaata 
        atagacact tctttggcc tcgttaatt tctaccaca 
        gaagaaatt tcttcatct ttagaatct tcatctaca 
        gaatcaacg acgcaagaa gatgtttca gtttcactt 
        acaccatac aacatgacg catgaaact ccaccaaaa 
        acttctcca tttccgacc aaaattaaa gaggcaact 
        tcaacgata ctagaacac agtcagtcc gtaccgccc 
        caacagagt aacgaacta ttaacatca tttcgtttc 
        gcgaataaa atatcccgt gacttggaa ggagtatca 
        aaagaacaa tcctcttca accataagc acacaaaaa 
        actacagaa actccatgt cttccaaca aaaattgat 
        gagacaatg acatcggaa gaaaatacg tctcttcca 
        atggaggaa aatgatctt tcggaatcg tttcagctg 
        ctcaataaa acgtttcta cagttagaa tcagccttt 
        gaaaatctg acttctgga aatgcaaaa acaagtggt 
        gttcaaggt ggtactgca aaagaagta cgcagaacc 
        actacagag gtggaaaag acatcagca aattacgaa 
        gtttcagaa actacctct gaaattgca actttttct 
        gcagtttcc gagatcata gttccaata atagaagta 
        acagaaact tcatcaacc tctagtact cctgcagaa 
        actgcagaa ttaacacta caaacatca tcatctcct 
        gtagctgaa gttagttct actgcagaa aatacttct 
        gaagaaacg acagtcttg atgtctgaa aagaccaca 
        aattttgcc tttgaaact acactcgaa actagtagg 
        gaaactgaa atgacgagt tccgttatt tcatcttca 
        aagttaacc ccaagtacg agcagtacc aaagaagca 
        tatgctgaa gaaaatttg gcgcaattt acaaaacct 
        attccaact agtgttttg aaaatggag ggggctaca 
        aacagcact ttttcattg atagcaata ccagaaact 
        agtgctaaa ttagaagaa acaagtccg caaacaaca 
        tcattcaac attgcaaca gaaaaacaa atgcttgaa 
        tcaaaagaa agcccaact gtgagcagt actatcaaa 
        gaagaattt gcgcacttt acaaaacct gttccaaca 
        agtcttttg aaacaggag gggggtaca gtcaccaca 
        tattcatcg atagcaacg ccagaaact agtgctagt 
        accgaaact gaaacaata attatgaca actggttct 
        actacttat tcacctcca caattagaa gaaacaagt 
        ccgcaaaca acatcattc aacattgct acagaaaac 
        caaatgcct gaatccaaa gaaagccca actgtgagc 
        agtactacc aaagaagaa tatgctgaa gaaaacttt 
        gtgcacttc acaaaacct gttccagca agtgttttg 
        aatatggag ggggaaaaa caaatgcct gaatccgag 
        gaaagccca actgtgagc agtactacc aaagaagaa 
        tatgctgaa gaaaacttt gagcacttc gcaaaacct 
        gttccaaca agtgttttg aaaatggaa gagattaca 
        accaacgaa tatttttca atcgcaaat gaagtgcct 
        ataatttct gctactcct atagaagat gtgaaattg 
        acttcattg gcaaagata cttttccct ccatatatc 
        cacatccca acatataaa acagacgag gttgaagac 
        aatgttcct aaagaaata aatccttta gtaactttt 
        tcaactact gaacttcaa aacggtcca acaatatat 
        gccccgtat gctcagatt ccaacctac agaacaaat 
        caatttcaa gaagtatca tcaccacct tttcctcct 
        cagtttact cctcttcct tccacatca aattcgcta 
        accacgtcg tcacaaaat ttggaagat gtgtctaac 
        gaatatgcc cactatcga gacgaagaa cttagtgct 
        attttgaat ttactaggg cacgatagc agtaccgtc 
        tccacggac aatgatact tgcggaggt atgcttgga 
        aaaatcaaa aggaaagtt tttagtaag ttgcacact 
        gctcaaaaa gaattgaaa ctagtttta gccgtaaaa 
        aatttactt cttaaatcg aaaaatcgg caaaaggag 
        catggaatc agaagaaaa aaagatgta catcttcgt 
        aatgatctt cgacctaaa aggaaaaga gtaccagac 
        tctaaggaa aaaagaatg aaacaagaa acagaagtc 
        ggcgaaaaa tcggaagaa gaggaggaa atgagcatt 
        aaaatgaac aatagatgt ggattctaa
      mRNA sequence
      • >TC009813 RA
        agccatatt ttcaaatgc ttcgctatg gtttaattt 
        acattgcca cttttatgt ttgtttgtg gggaatata 
        gcgcaataa aagaccaag cgatgcggt catacgatg 
        ataaattct cagcagaaa tttttgaca ataagcaag 
        aacctagag aaaacactg atccctcaa accaaaaac 
        gtccaactt gtgcacaaa aaaacaaaa acaatggga 
        acaaaatag acaatttag atatagacg aaaaagaat 
        tggcgaaaa tcggtaaac tgttcatgc accatttgg 
        ccaaagatg ggcggaaaa gggttgata ccaatgatg 
        taccgatgg aagaacctg acgaagaag accacgggc 
        aatacgtcg atcaagatt ttcttgacg aaatgcaaa 
        aaattgaga atattacta atgaaggca acaagcttt 
        acgacgaac tcggaggtc ccccaacaa tcaaatatg 
        acccagatc ccgacaatt tgaaaagta tttgctgca 
        ctgaagacg aatcactta tcgatgatt caaacacaa 
        atcatcacc atttaaaaa tgcattttt acaagttta 
        gtcccgaaa aaatcgatg ataagagag cgattagtt 
        tataccccg acaggaatc agttttata agatacccc 
        aaaaattta attacatcg cttttctat caaacgacg 
        acaaattca tggatgttc ctgcggtca aattgaatg 
        ttttttcag acgatttga aacggggca agcgaaccg 
        aaacttttg ttccttttt ttaccactc tatacaaga 
        gaagcgcca aaaataaat taaaattca ggcccctac 
        gaaaacgct tcacacaca ctcctgttc tgtttttca 
        acacaattt ctggtgcta aaagataca gacgccttg 
        ctaaaacaa cttcaaaac ccgcggttt cgaatgtta 
        tgaaagacg ctgatgcat tgctaaata aagtaaact 
        tttttctaa agttgggag acgaagaaa acgaaatac 
        gtcaaattt ccgctcatg tttttccaa aaaaacaag 
        ttaaaaaag aaattaaaa aaaacaatg ggtttaaag 
        aatggttcg aggatgatt atgaagagc cacggacaa 
        aaaaagaga gccaaaatc agttaggaa cttcttcac 
        ttaaagaaa atttcaatt tacttctgg attttgatg 
        gtgaattaa aaacaacag aagctacga caatgagac 
        aattgacca caacggcgt gcaagaaaa atgcaatag 
        acatgtcac ccattgaaa tcgaaaata tgatgaagg 
        acgttgttg cgaaaatag atcttcctt ctttggcca 
        gaaatgact ttgcgagtc ggggatatc aaaacaaat 
        tgctaaatt ctgaagaat caggatcgt ccaaagaag 
        atttggtaa atgatttta ttaaagacg ataatttta 
        aaagcgaaa taaatccag aaaataata atctaggcg 
        aaacgatag tcgcagagc cacccattt tcggtaatt 
        ctttactgt atgatatca acgaaaata aaattcaaa 
        gtgacatag aaaatacaa ccgatgcac aaactacgg 
        aaaaaccag gaaatttat cgtgcgctt atttactcg 
        atcttttta aaaatatcg aagcattga agaaaacag 
        atccaaatt tgcaaaaaa tatgtccta aattaagtg 
        aactctcac acatgggtg aaaattcaa caaccacag 
        aagaatgtc tgaacaaaa cgtcggtag taacgaatg 
        aaacaactg taaaaatgg aagtaacaa caccagaaa 
        ctacgtcaa ctttcgttt gtgtaacta cagaaactt 
        cttcggtgt cgtcgtctt taacaacgc aagaaatta 
        cttcaccta cttcttgta ctacagaat caacaatag 
        acacttctt cggttccgt cgtctttag caacggaag 
        aaattactt cacctactt cttctacta cagaatcaa 
        caacaatag acacttctc cggtttcct catctttaa 
        caacgcaag aaattactt cagctactt cttggacta 
        cagaatcag caataatag acacttctt tggcctcgt 
        taatttcta ccacagaag aaatttctt catctttag 
        aatcttcat ctacagaat caacgacgc aagaagatg 
        tttcagttt cacttacac catacaaca tgacgcatg 
        aaactccac caaaaactt ctccatttc cgaccaaaa 
        ttaaagagg caacttcaa cgatactag aacacagtc 
        agtccgtac cgccccaac agagtaacg aactattaa 
        catcatttc gtttcgcga ataaaatat cccgtgact 
        tggaaggag tatcaaaag aacaatcct cttcaacca 
        taagcacac aaaaaacta cagaaactc catgtcttc 
        caacaaaaa ttgatgaga caatgacat cggaagaaa 
        atacgtctc ttccaatgg aggaaaatg atctttcgg 
        aatcgtttc agctgctca ataaaacgt ttctacagt 
        tagaatcag cctttgaaa atctgactt ctggaaatg 
        caaaaacaa gtggtgttc aaggtggta ctgcaaaag 
        aagtacgca gaaccacta cagaggtgg aaaagacat 
        cagcaaatt acgaagttt cagaaacta cctctgaaa 
        ttgcaactt tttctgcag tttccgaga tcatagttc 
        caataatag aagtaacag aaacttcat caacctcta 
        gtactcctg cagaaactg cagaattaa cactacaaa 
        catcatcat ctcctgtag ctgaagtta gttctactg 
        cagaaaata cttctgaag aaacgacag tcttgatgt 
        ctgaaaaga ccacaaatt ttgcctttg aaactacac 
        tcgaaacta gtagggaaa ctgaaatga cgagttccg 
        ttatttcat cttcaaagt taaccccaa gtacgagca 
        gtaccaaag aagcatatg ctgaagaaa atttggcgc 
        aatttacaa aacctattc caactagtg ttttgaaaa 
        tggaggggg ctacaaaca gcacttttt cattgatag 
        caataccag aaactagtg ctaaattag aagaaacaa 
        gtccgcaaa caacatcat tcaacattg caacagaaa 
        aacaaatgc ttgaatcaa aagaaagcc caactgtga 
        gcagtacta tcaaagaag aatttgcgc actttacaa 
        aacctgttc caacaagtc ttttgaaac aggaggggg 
        gtacagtca ccacatatt catcgatag caacgccag 
        aaactagtg ctagtaccg aaactgaaa caataatta 
        tgacaactg gttctacta cttattcac ctccacaat 
        tagaagaaa caagtccgc aaacaacat cattcaaca 
        ttgctacag aaaaccaaa tgcctgaat ccaaagaaa 
        gcccaactg tgagcagta ctaccaaag aagaatatg 
        ctgaagaaa actttgtgc acttcacaa aacctgttc 
        cagcaagtg ttttgaata tggaggggg aaaaacaaa 
        tgcctgaat ccgaggaaa gcccaactg tgagcagta 
        ctaccaaag aagaatatg ctgaagaaa actttgagc 
        acttcgcaa aacctgttc caacaagtg ttttgaaaa 
        tggaagaga ttacaacca acgaatatt tttcaatcg 
        caaatgaag tgcctataa tttctgcta ctcctatag 
        aagatgtga aattgactt cattggcaa agatacttt 
        tccctccat atatccaca tcccaacat ataaaacag 
        acgaggttg aagacaatg ttcctaaag aaataaatc 
        ctttagtaa ctttttcaa ctactgaac ttcaaaacg 
        gtccaacaa tatatgccc cgtatgctc agattccaa 
        cctacagaa caaatcaat ttcaagaag tatcatcac 
        caccttttc ctcctcagt ttactcctc ttccttcca 
        catcaaatt cgctaacca cgtcgtcac aaaatttgg 
        aagatgtgt ctaacgaat atgcccact atcgagacg 
        aagaactta gtgctattt tgaatttac tagggcacg 
        atagcagta ccgtctcca cggacaatg atacttgcg 
        gaggtatgc ttggaaaaa tcaaaagga aagttttta 
        ccgtaaaaa atttacttc ttaaatcga aaaatcggc 
        aaaaggagc atggaatca gaagaaaaa aagatgtac 
        atcttcgta atgatcttc gacctaaaa ggaaaagag 
        taccagact ctaaggaaa aaagaatga aacaagaaa 
        cagaagtcg gcgaaaaat cggaagaag aggaggaaa 
        tgagcatta aaatgaaca atagatgtg gattctaaa 
        cacaaagga gttcaaagg aagaccata caacaaagt 
        gtctaatca cttaatttt aagtcataa caattgaaa 
        atatgatga taggcgtga aaatgctat attaaatac 
        agattttaa ttgagctgg gttttgtat tgtttgtaa 
        ctttataag aaaacatat ggttgtggc actgtcacc 
        tttactttt tgaatttgg tgtaacaaa atcttcagt 
        cgaaatttt aaacatgaa gctgcttta aattattat 
        ttggagact aacttttat tttaataaa gaatcaaat 
        cagtt
      • >TC009813 RB
        tttttgtgt aagccatat tttcaaatg cttcgctat 
        ggtttaatt tacattgcc acttttatg tttgtttgt 
        ggggaatat agcgcaata aaagaccaa gcgatgcgg 
        tcatacgat gataaattc tcagcagaa atttttgac 
        aataagcaa gaacctaga gaaaacact gatccctca 
        aaccaaaaa cgtccaact tgtgcacaa aaaaacaaa 
        aacaatggg aacaaaata gacaattta gatatagac 
        gaaaaagaa ttggcgaaa atcggtaaa ctgttcatg 
        caccatttg gccaaagat gggcggaaa agggttgat 
        accaatgat gtaccgatg gaagaacct gacgaagaa 
        gaccacggg caatacgtc gatcaagat tttcttgac 
        gaaatgcaa aaaattgag aatattact aatgaaggc 
        aacaagctt tacgacgaa ctcggaggt cccccaaca 
        atcaaatat gacccagat cccgacaat ttgaaaagt 
        atttgctgc actgaagac gaatcactt atcgatgat 
        tcaaacaca aatcatcac catttaaaa atgcatttt 
        tacaagttt agtcccgaa aaaatcgat gataagaga 
        gcgattagt ttatacccc gacaggaat cagttttat 
        aagataccc caaaaattt aattacatc gcttttcta 
        tcaaacgac gacaaattc atggatgtt cctgcggtc 
        aaattgaat gttttttca gacgatttg aaacggggc 
        aagcgaacc gaaactttt gttcctttt tttaccact 
        ctatacaag agaagcgcc aaaaataaa ttaaaattc 
        aggccccta cgaaaacgc ttcacacac actcctgtt 
        ctgtttttc aacacaatt tctggtgct aaaagatac 
        agacgcctt gctaaaaca acttcaaaa cccgcggtt 
        tcgaatgtt atgaaagac gctgatgca ttgctaaat 
        aaagtaaac ttttttcta aagttggga gacgaagaa 
        aacgaaata cgtcaaatt tccgctcat gtttttcca 
        aaaaaacaa gttaaaaaa gaaattaaa aaaaacaat 
        gggtttaaa gaatggttc gaggatgat tatgaagag 
        ccacggaca aaaaaagag agccaaaat cagttagga 
        acttcttca cttaaagaa aatttcaat ttacttctg 
        gattttgat ggtgaatta aaaacaaca gaagctacg 
        acaatgaga caattgacc acaacggcg tgcaagaaa 
        aatgcaata gacatgtca cccattgaa atcgaaaat 
        atgatgaag gacgttgtt gcgaaaata gatcttcct 
        tctttggcc agaaatgac tttgcgagt cggggatat 
        caaaacaaa ttgctaaat tctgaagaa tcaggatcg 
        tccaaagaa gatttggta aatgatttt attaaagac 
        gataatttt aaaagcgaa ataaatcca gaaaataat 
        aatctaggc gaaacgata gtcgcagag ccacccatt 
        ttcggtaat tctttactg tatgatatc aacgaaaat 
        aaaattcaa agtgacata gaaaataca accgatgca 
        caaactacg gaaaaacca ggaaattta tcgtgcgct 
        tatttactc gatcttttt aaaaatatc gaagcattg 
        aagaaaaca gatccaaat ttgcaaaaa atatgtcct 
        aaattaagt gaactctca cacatgggt gaaaattca 
        acaaccaca gaagaatgt ctgaacaaa acgtcggta 
        gtaacgaat gaaacaact gtaaaaatg gaagtaaca 
        acaccagaa actacgtca actttcgtt tgtgtaact 
        acagaaact tcttcggtg tcgtcgtct ttaacaacg 
        caagaaatt acttcacct acttcttgt actacagaa 
        tcaacaata gacacttct tcggttccg tcgtcttta 
        gcaacggaa gaaattact tcacctact tcttctact 
        acagaatca acaacaata gacacttct ccggtttcc 
        tcatcttta acaacgcaa gaaattact tcagctact 
        tcttggact acagaatca gcaataata gacacttct 
        ttggcctcg ttaatttct accacagaa gaaatttct 
        tcatcttta gaatcttca tctacagaa tcaacgacg 
        caagaagat gtttcagtt tcacttaca ccatacaac 
        atgacgcat gaaactcca ccaaaaact tctccattt 
        ccgaccaaa attaaagag gcaacttca acgatacta 
        gaacacagt cagtccgta ccgccccaa cagagtaac 
        gaactatta acatcattt cgtttcgcg aataaaata 
        tcccgtgac ttggaagga gtatcaaaa gaacaatcc 
        tcttcaacc ataagcaca caaaaaact acagaaact 
        ccatgtctt ccaacaaaa attgatgag acaatgaca 
        tcggaagaa aatacgtct cttccaatg gaggaaaat 
        gatctttcg gaatcgttt cagctgctc aataaaacg 
        tttctacag ttagaatca gcctttgaa aatctgact 
        tctggaaat gcaaaaaca agtggtgtt caaggtggt 
        actgcaaaa gaagtacgc agaaccact acagaggtg 
        gaaaagaca tcagcaaat tacgaagtt tcagaaact 
        acctctgaa attgcaact ttttctgca gtttccgag 
        atcatagtt ccaataata gaagtaaca gaaacttca 
        tcaacctct agtactcct gcagaaact gcagaatta 
        acactacaa acatcatca tctcctgta gctgaagtt 
        agttctact gcagaaaat acttctgaa gaaacgaca 
        gtcttgatg tctgaaaag accacaaat tttgccttt 
        gaaactaca ctcgaaact agtagggaa actgaaatg 
        acgagttcc gttatttca tcttcaaag ttaacccca 
        agtacgagc agtaccaaa gaagcatat gctgaagaa 
        aatttggcg caatttaca aaacctatt ccaactagt 
        gttttgaaa atggagggg gctacaaac agcactttt 
        tcattgata gcaatacca gaaactagt gctaaatta 
        gaagaaaca agtccgcaa acaacatca ttcaacatt 
        gcaacagaa aaacaaatg cttgaatca aaagaaagc 
        ccaactgtg agcagtact atcaaagaa gaatttgcg 
        cactttaca aaacctgtt ccaacaagt cttttgaaa 
        caggagggg ggtacagtc accacatat tcatcgata 
        gcaacgcca gaaactagt gctagtacc gaaactgaa 
        acaataatt atgacaact ggttctact acttattca 
        cctccacaa ttagaagaa acaagtccg caaacaaca 
        tcattcaac attgctaca gaaaaccaa atgcctgaa 
        tccaaagaa agcccaact gtgagcagt actaccaaa 
        gaagaatat gctgaagaa aactttgtg cacttcaca 
        aaacctgtt ccagcaagt gttttgaat atggagggg 
        gaaaaacaa atgcctgaa tccgaggaa agcccaact 
        gtgagcagt actaccaaa gaagaatat gctgaagaa 
        aactttgag cacttcgca aaacctgtt ccaacaagt 
        gttttgaaa atggaagag attacaacc aacgaatat 
        ttttcaatc gcaaatgaa gtgcctata atttctgct 
        actcctata gaagatgtg aaattgact tcattggca 
        aagatactt ttccctcca tatatccac atcccaaca 
        tataaaaca gacgaggtt gaagacaat gttcctaaa 
        gaaataaat cctttagta actttttca actactgaa 
        cttcaaaac ggtccaaca atatatgcc ccgtatgct 
        cagattcca acctacaga acaaatcaa tttcaagaa 
        gtatcatca ccacctttt cctcctcag tttactcct 
        cttccttcc acatcaaat tcgctaacc acgtcgtca 
        caaaatttg gaagatgtg tctaacgaa tatgcccac 
        tatcgagac gaagaactt agtgctatt ttgaattta 
        ctagggcac gatagcagt accgtctcc acggacaat 
        gatacttgc ggaggtatg cttggaaaa atcaaaagg 
        aaagttttt agtaagttg cacactgct caaaaagaa 
        ttgaaacta gttttagcc gtaaaaaat ttacttctt 
        aaatcgaaa aatcggcaa aaggagcat ggaatcaga 
        agaaaaaaa gatgtacat cttcgtaat gatcttcga 
        cctaaaagg aaaagagta ccagactct aaggaaaaa 
        agaatgaaa caagaaaca gaagtcggc gaaaaatcg 
        gaagaagag gaggaaatg agcattaaa atgaacaat 
        agatgtgga ttctaaaca caaaggagt tcaaaggaa 
        gaccataca acaaagtgt ctaatcact taattttaa 
        gtcataaca attgaaaat atgatgata ggcgtgaaa 
        atgctatat taaatacag attttaatt gagctgggt 
        tttgtattg tttgtaact ttataagaa aacatatgg 
        ttgtgg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY