Status
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Gene information
General information
TC009802 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8301530..8322500
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009802
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01611

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009802 RA
      METIAEDQE TEYELDKVK KKYASLGHT ILMVQLERS 
      SQGLGLSLA GHKDRNCMA VFVCGLNPN GAAYKTGGI 
      QIGDEILEV NGVVLHGRC HLNASAIIK GLSGPIFKV 
      IILRRKTAI DDIAVKPIT QFPVSLAEE ASEEQFSAS 
      YPNVRTVAI KKGNQSLGI MIIEGKHAE VGQGIFISD 
      IQEGSSAEK AGLEIGEMI LAVNKDSLV GSNYDTAAN 
      LLKRTEGLV NLVVSNPGK KDSAVTQAN AVDNTKQNA 
      VSKPTLKPS SGPPSRPST PVPEPAVDL TTCPITPGK 
      DVAIEIPTD NKGLGVFFV GGKDTAMPN GIVIVEVYP 
      GGAADRDSR LQAGDQILE VNGTQLKDV THTTAAQAL 
      RQTLPKMKL VVYRPERVD FTKLDVELT KKPGKGMGL 
      SVIARKSGK GVYIGDIIN GGTADVDGR IMKGDLLVS 
      VNGQSVENS SRDEAGAIL KTVTGRVSL KLHRYKPVA 
      R
    CDS sequence
    • >TC009802 RA
      atggaaacc atagccgag gatcaggaa acagagtat 
      gaactcgac aaggtgaag aagaaatac gccagcctg 
      ggtcacaca atccttatg gtccaactg gagcgcagc 
      agtcaaggt ttgggcctg agtttggcc ggtcacaag 
      gaccgcaat tgcatggcc gttttcgtg tgcggcctg 
      aatccgaac ggcgcggct tacaagacg ggaggcatc 
      caaatcggc gacgagata ctcgaggtg aatggtgtg 
      gttttacat ggacgctgt catttgaac gcttccgct 
      atcattaaa ggcctttct ggaccgatt tttaaagtc 
      attatatta aggcggaaa actgccatc gatgacatc 
      gcagttaag cccatcaca caatttcca gtttccttg 
      gcggaggag gcctccgag gagcagttt agcgctagc 
      tatcccaat gtacgaacc gtcgctatc aaaaagggc 
      aaccagagc ttaggtata atgataata gaaggcaag 
      catgcggaa gtgggccaa ggtatattc atctcggac 
      atccaagag ggcagttca gccgaaaag gccggccta 
      gaaatcggc gaaatgatc ctggccgtt aataaagac 
      tccctagtc ggctccaat tacgacacc gccgccaac 
      ttgctgaag aggaccgag ggtttggtg aacctcgtt 
      gtgtccaat ccggggaaa aaagattcg gcggtaacg 
      caagccaac gccgttgac aacaccaaa caaaacgcc 
      gtttccaaa cccactctt aaaccttct tccgggccc 
      ccgtcgaga cccagcacg ccagtacct gaacccgcc 
      gtagatctc acgacatgt ccgataacg ccgggaaaa 
      gacgtcgcg atcgaaatc ccaacggac aacaaaggc 
      ttgggcgta ttcttcgta ggcggcaaa gacaccgcg 
      atgccgaac ggaatcgtg atcgtggaa gtgtatcct 
      ggcggagcg gccgacagg gatagccgc ctccaagcc 
      ggcgaccag atcctggag gtcaacggg acccaactc 
      aaggacgta acacacacc accgccgct caggcgctg 
      agacaaaca ctgccaaag atgaaactg gtcgtctat 
      cgaccggag agggtcgac ttcaccaag ctggacgtg 
      gagctgacc aagaagccc ggcaagggt atggggctg 
      agcgtcatc gcccggaaa tccgggaag ggcgtctac 
      atcggcgac attataaat ggagggacc gccgacgtg 
      gacgggagg ataatgaaa ggggaccta ttagtgtct 
      gtgaacggg caaagtgtc gaaaattcg tcacgtgat 
      gaagccggg gccattctt aagactgtc acgggcaga 
      gtttcactc aaattacac agatataaa ccggtcgcg 
      agataa
    mRNA sequence
    • >TC009802 RA
      actcatcca attatttat ctgtatgta catcttgca 
      aacatatct ttttatagt gttctctaa aaattatag 
      tttgacaaa agacgaaat atgttttcg tccacatca 
      ggtgttaag tgtgcgaaa aactattgt tcaaagtca 
      gtttccgtg taagaaatg taaatgtca catctggac 
      agttttctg atttcagca cacgtgtga gagacagtg 
      gataaaata tcgcgtcgt ttttactct ttcgaacat 
      tattaaatt ctcttattc ctttcgtcc ctatcgatt 
      gcaatcgtt ctgcgttcg atgacctcc ttttggtca 
      tctgcaaag aaaaaatcg accgctctg aagcaggaa 
      ctctaattg tttaaaata tgcctgaac tgtttagat 
      tacgggaga atctgtcga tttgataat agcctgttt 
      attacacta aaagttgtt taaaattct attataaaa 
      caatttggt tttcacttt taagaatag caaatagtg 
      tatcttctg gtcatcaaa aacaaatta tccttcatg 
      gccgtcggc gtcttcacc aattttttc gtcgattcg 
      aaataattc acttttctt atctacact ctggtccat 
      tatttttaa cttagcgtt ttagaagcg ctctaaacg 
      tcccgataa aataataaa gttatttgt ttcgatacg 
      aaggctttt agctttgac acaattacg tttcttatt 
      tttaattcc acaaagaaa cttttttcg tctcgactc 
      gcgcacacc tacttgaaa taaagtagc aataacgag 
      attgagggg agaaacttt tccgagttt ttgtttgaa 
      cgatattta ataacaaag ggtggacgt gggaagaaa 
      aatgtcatt tttttgaaa cgacgagat ttgtcctcg 
      tctaggatt aaataaaag ttcgttgaa cgcatcatt 
      aaataccgg ggaaacttc cagggttct atttatttc 
      acctttaca caatttacg gcttcgtaa tggatactt 
      aaaggtcct gagacctgg gtgtaaatt tgaatgggt 
      ttcattatt tgctgtttg ctgggtatt agcaaactc 
      tgaaaattt cataaatga gttacgttt aaaccgatt 
      ttagggctc ctatagctt gggtctttt aacacattg 
      cgatcgttg ggaattatt tattatgta aaaactgtg 
      ccaggggag cgcgaaaac aacaagaat tatgtcaga 
      ctattcttt tcaaaacac ctgtgagag actttttcc 
      tttggtttc ggttttatg cttatgacg atgatttcc 
      tgcaaactg caaatcgtc aaattctta cgacagttt 
      tagtgaaat gtttacgaa tgcaaattt acttgtacg 
      ctgtccata cgggcaaag tttgcattt tctgtttta 
      tttctttgt tgttccgat ttgattgca acaataaag 
      acaatgccg acatagcaa acatgtctg gcacttata 
      catacatga ataaaatga atcggtcat tatttattt 
      acctttgta attgtagtt ttcaatctt tgcaagaat 
      cctcttttt catcccgaa ccccgcatt atttatgca 
      ttttaatta cgtcgattc tacgtcaat aaattcgtt 
      ccgtgggtc atcgcaatt ggacccgac cgtcaagta 
      tcgttgcgt gtgtatcgc taaaatgtc ccattaaaa 
      tattcactc gcttttctc cgaatcaat ttttttcgt 
      tatttatat cccgacctt cctcctgtc gataacgcg 
      acgttcctc atcgtcgtc ggcggtttt caggagtgc 
      gactgaatt tcccggaaa gcttttccc tgttacaca 
      cttagacga tgatcgacg cgaattgac gccagcagg 
      tgttcgatc gtttgtacg cgcgatggc ctccaacac 
      catcatcag tgactcgaa acgtgctaa aacgaccaa 
      aagtcgaaa agttttaaa gaaaattgt ggcgaaacg 
      gtagtgatg caatcacga tcatggaac catggaaac 
      catagccga ggatcagga aacagagta tgaactcga 
      caaggtgaa gaagaaata cgccagcct gggtcacac 
      aatccttat ggtccaact ggagcgcag cagtcaagg 
      tttgggcct gagtttggc cggtcacaa ggaccgcaa 
      ttgcatggc cgttttcgt gtgcggcct gaatccgaa 
      cggcgcggc ttacaagac gggaggcat ccaaatcgg 
      cgacgagat actcgaggt gaatggtgt ggttttaca 
      tggacgctg tcatttgaa cgcttccgc tatcattaa 
      aggcctttc tggaccgat ttttaaagt cattatatt 
      aaggcggaa aactgccat cgatgacat cgcagttaa 
      gcccatcac acaatttcc agtttcctt ggcggagga 
      ggcctccga ggagcagtt tagcgctag ctatcccaa 
      tgtacgaac cgtcgctat caaaaaggg caaccagag 
      cttaggtat aatgataat agaaggcaa gcatgcgga 
      agtgggcca aggtatatt catctcgga catccaaga 
      gggcagttc agccgaaaa ggccggcct agaaatcgg 
      cgaaatgat cctggccgt taataaaga ctccctagt 
      cggctccaa ttacgacac cgccgccaa cttgctgaa 
      gaggaccga gggtttggt gaacctcgt tgtgtccaa 
      tccggggaa aaaagattc ggcggtaac gcaagccaa 
      cgccgttga caacaccaa acaaaacgc cgtttccaa 
      acccactct taaaccttc ttccgggcc cccgtcgag 
      acccagcac gccagtacc tgaacccgc cgtagatct 
      cacgacatg tccgataac gccgggaaa agacgtcgc 
      gatcgaaat cccaacgga caacaaagg cttgggcgt 
      attcttcgt aggcggcaa agacaccgc gatgccgaa 
      cggaatcgt gatcgtgga agtgtatcc tggcggagc 
      ggccgacag ggatagccg cctccaagc cggcgacca 
      gatcctgga ggtcaacgg gacccaact caaggacgt 
      aacacacac caccgccgc tcaggcgct gagacaaac 
      actgccaaa gatgaaact ggtcgtcta tcgaccgga 
      gagggtcga cttcaccaa gctggacgt ggagctgac 
      caagaagcc cggcaaggg tatggggct gagcgtcat 
      cgcccggaa atccgggaa gggcgtcta catcggcga 
      cattataaa tggagggac cgccgacgt ggacgggag 
      gataatgaa aggggacct attagtgtc tgtgaacgg 
      gcaaagtgt cgaaaattc gtcacgtga tgaagccgg 
      ggccattct taagactgt cacgggcag agtttcact 
      caaattaca cagatataa accggtcgc gagataatt 
      ccacggact gtttatagt gtgctaagc caaattttt 
      caagttgat attatttcc atcgagagg tgtctttgc 
      caaaaattc gattgcgtt tgcatttaa cggtttatt 
      gtcgcattt aattcgatg atatctgtg tattattaa 
      cattcatct agtcactta agttattcg gtacttaat 
      ttattatct aagtatatc tattttgta aaaatagta 
      tacctgctt cgattgtga taaaaacta aaaatttag 
      aaaaataaa ttatattta actaagtca tttatggaa 
      gatttttag ggtaatatg tcgagttcc aatgtattt 
      ttcaatgat tttttgtta tccagaaaa tcgtaggat 
      caagtattt cattaaaat cgcgtaaaa gtaatattt 
      atatagatg attacaata aagagatgt gcatgtatt 
      tatcagttt agaacgcat tactatttt ttaggtata 
      ttcattagt tcgttatat aaattttaa aagaaatta 
      aataatgta ttgaacaga ctgtaataa tttgtaaat 
      actgtattg tcttgaaaa agttcacta cttgtacta 
      aatctagaa ctattttgt aaatagggt tatgcaatg 
      gtgcatgct ggaatttta ataaaattc actttatct 
      c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY