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Gene information
General information
TC009800 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 1579223..1584418
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009800
    iBeetle screen iB_01610
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    iBeetle screen iB_06751
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 10 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009800 RA
      MLDRDLARS QPKSSVVSS TPMPFSRHK NFLPNASNS 
      VSPITVVKP GNATKPAKA STSANSRTN LFKAEISTP 
      ITVSSTYDD DANSVSELD TSIRSQRLE NLVLSLHRS 
      NENIAASLR AEPVVNNQR LNKNASTMS RKNANIFET 
      TDKMVQTSN NNSLAGAKK LSRSRRSNC DLSFSRSEE 
      LDFEKMVTE KRLADLIDK LEDNFLKRP PVESRMENF 
      SRIDTGVPW RATIVPHEN VALNANSLL AFGSSDEES 
      FDDTFLPKM RPKPKVFVN KKKLKRGSG RFLKNATKN 
      AKQSKLKLD ERSMTSSVA SEVPQTADQ GRMKSRAKG 
      KEKERVETL IKEPEENPG VKNKKRQSR SPTKSQTKH 
      NVLDANHFQ EVTSNSELL TERFLNEEN KKVESTRKG 
      QKKRDRTES PLKKLRRSD INDSKSGKQ NMTQDDGEM 
      VSYGTNTDN WRNDLFGES SMKEMEHLE KGKKSGQNL 
      KTEKKKQNA SPSKKQKAI VKCLANKNK EHSVTDDSG 
      SDESQRVSV KAVVHASAT QKNSKKPAG TQKQQLRNV 
      DNIHILESG LDVMTNSDE ETSREVEAF FESNNQDTN 
      FGAPNLDNL QQKPSLNNS VNKSAEKST ARKTQNKKG 
      KKQTKRSTT KSKAQSTTG ITETTTDDT TGCMQRRSG 
      RQRKQTKIN SAGYILCTE RDRYYKVAT AVRHISQVT 
      PECSFRLSG LQKRRTRIT QMPPIMESE VEMEGNTQK 
      SKRKTKKPA KKQSSKKLK KDETPKRQE TRTVSESET 
      NLTRSDYIV GENVITSDY VSAENVAQN TQIPFVRLE 
      EITSRNIGS EKLHNTVFS SPPLQAQES IKKNSVISG 
      RISKRSSTK TSAKKVRDS LKKVVETQR DSSGNFMIC 
      PATNELIRY SFKNPVYKP IANLPGVTY AVTEAVKLG 
      WLKISGGMS KPLFKTKNH TMSFVVVEG KAEVGIQDK 
      CDLYKKHDI FIVPQGAEY YIDNKNKTK PLLLVFFKD 
      AYADKPT
    CDS sequence
    • >TC009800 RA
      atgttggac cgagacctg gctcgctcg caaccaaaa 
      tcttcagtt gtgagcagt accccaatg ccatttagt 
      cggcacaaa aactttctt ccgaatgcc agtaattca 
      gtgtcaccc attaccgtc gtcaaaccg ggcaacgca 
      accaaaccg gcgaaagcg agcacatca gcgaactca 
      cgtaccaat ctcttcaaa gcagaaata tcgacaccg 
      atcacggtg tcgtcaacc tacgacgac gatgcgaat 
      tcggtgtct gagctcgac acttcgatc agaagccag 
      cgcttggaa aaccttgtg ttgtcgtta caccgaagc 
      aacgagaat attgcggcg tctcttcgc gccgaacct 
      gtggtcaac aaccagcgc ttgaataaa aatgcctcg 
      acaatgtct cggaaaaac gcaaatatt ttcgaaacc 
      actgataaa atggtgcaa acttccaat aataattca 
      ctagcgggg gcgaaaaag ctgtcaagg agcaggaga 
      tccaactgt gatctttca ttctctagg agtgaagaa 
      ctcgatttt gagaaaatg gtcacggag aagaggctc 
      gcagatttg atcgacaag ttagaagac aattttttg 
      aagagaccg cctgtggaa tcccgaatg gagaatttt 
      tcgagaatt gatactgga gtaccatgg cgagcgacg 
      attgtacct cacgaaaat gttgcgtta aatgcaaat 
      tctttacta gcgtttgga agttccgat gaggagtct 
      tttgatgac actttccta ccgaaaatg cgcccgaaa 
      cccaaagtt ttcgttaat aaaaagaag ttgaaaagg 
      ggttcggga agatttttg aagaatgca acgaaaaac 
      gcaaagcag agtaaacta aagctggat gaaagaagt 
      atgactagt tcagttgca agtgaagta cctcaaacg 
      gcggaccaa ggacggatg aaaagtagg gcaaaggga 
      aaggaaaaa gaaagggtt gaaacttta ataaaggaa 
      ccagaagag aatccagga gttaaaaat aaaaagaga 
      caaagccga agtccaact aaatcacag acgaaacat 
      aacgtacta gacgctaat cattttcaa gaagtgacg 
      tctaatagt gaactatta actgaacgt tttttgaac 
      gaggaaaat aaaaaggtt gagagtaca cggaaagga 
      cagaagaaa cgggatagg actgaaagt ccgctcaaa 
      aaattaaga agatcagat ataaacgat tccaagagt 
      ggcaagcaa aatatgaca caagacgat ggagaaatg 
      gtgtcatat ggtacaaat acagataac tggagaaat 
      gacttattt ggtgaaagt tcgatgaaa gaaatggaa 
      catttggag aagggaaag aaatcaggg caaaattta 
      aagacagaa aaaaagaaa cagaacgca agtccatca 
      aaaaaacaa aaagcaata gtaaagtgt cttgcaaat 
      aaaaataag gaacacagt gtcactgat gacagtggt 
      tccgatgaa tcgcaaaga gtgtcagtt aaggcggtc 
      gtacatgca agtgctaca caaaaaaat agtaaaaag 
      ccagcagga acacaaaaa caacaacta agaaatgta 
      gacaatata catattttg gaatcagga ttagacgta 
      atgactaat agtgatgaa gaaacgtca cgcgaagtt 
      gaagcattt tttgaatca aataatcag gatactaat 
      ttcggtgca ccgaatctg gacaatttg caacagaaa 
      ccatcacta aataatagc gttaataaa tcagctgaa 
      aaaagtaca gcgaggaaa acacaaaat aaaaaggga 
      aaaaagcag acaaaacgt tctacaacg aagtctaaa 
      gcccaaagt acaacagga attactgaa acgacaact 
      gacgacaca acaggatgt atgcaaagg agatcggga 
      aggcaacga aaacaaaca aagattaat tcagctggt 
      tacatccta tgcactgaa agggacaga tattataaa 
      gtcgcaacg gcggtaagg catatatca caagtaacg 
      cctgaatgt tcatttcga ctttcggga ctacaaaaa 
      cggcgtact cgtataact caaatgcca ccaattatg 
      gagtcggaa gttgaaatg gaaggtaat actcaaaag 
      tcgaaaagg aaaactaag aaaccggct aaaaaacaa 
      tcaagtaag aaacttaag aaagatgag accccaaaa 
      cgtcaagaa actaggact gtttctgaa tcggagaca 
      aatctaacc agaagtgat tacattgtt ggagaaaat 
      gtaattacc tccgattat gttagtgca gaaaatgtt 
      gcacaaaac actcaaata ccatttgta agactagaa 
      gaaattact tcaaggaac attggttct gaaaaactt 
      cataacacc gtcttttct tcaccaccg ttgcaagca 
      caagagagt atcaagaaa aattctgtt atatctgga 
      agaatatct aaacggtct agtaccaaa accagcgcg 
      aaaaaagtt agggattcc ttgaaaaag gtagtcgag 
      actcaacgc gactcaagc ggtaatttc atgatctgt 
      ccagcaact aatgagctt attagatac agtttcaaa 
      aatccggtg tacaaaccg atcgctaac ttgcctggt 
      gtcacgtac gctgtgacg gaagctgta aaactggga 
      tggttgaag ataagcgga ggaatgtca aagccatta 
      tttaaaact aagaatcac actatgtcg ttcgtagtt 
      gtagaagga aaagcggag gtggggata caggacaag 
      tgtgatttg tataaaaaa catgacata tttattgtt 
      ccacaagga gcggaatat tacatagat aacaaaaat 
      aaaactaag cccttgcta ttggttttc ttcaaagac 
      gcatatgcg gacaaaccg acgtaa
    mRNA sequence
    • >TC009800 RA
      atggaaatc attcgatta aaatgtttg gtagctttg 
      tacaacgaa atctaatcc acgaactaa agaaatcgt 
      ccaacggtc caaatcttt tttcaaaaa atgaggcaa 
      acagacaat gcaaattac gcccctcga tgttggacc 
      gagacctgg ctcgctcgc aaccaaaat cttcagttg 
      tgagcagta ccccaatgc catttagtc ggcacaaaa 
      actttcttc cgaatgcca gtaattcag tgtcaccca 
      ttaccgtcg tcaaaccgg gcaacgcaa ccaaaccgg 
      cgaaagcga gcacatcag cgaactcac gtaccaatc 
      tcttcaaag cagaaatat cgacaccga tcacggtgt 
      cgtcaacct acgacgacg atgcgaatt cggtgtctg 
      agctcgaca cttcgatca gaagccagc gcttggaaa 
      accttgtgt tgtcgttac accgaagca acgagaata 
      ttgcggcgt ctcttcgcg ccgaacctg tggtcaaca 
      accagcgct tgaataaaa atgcctcga caatgtctc 
      ggaaaaacg caaatattt tcgaaacca ctgataaaa 
      tggtgcaaa cttccaata ataattcac tagcggggg 
      cgaaaaagc tgtcaagga gcaggagat ccaactgtg 
      atctttcat tctctagga gtgaagaac tcgattttg 
      agaaaatgg tcacggaga agaggctcg cagatttga 
      tcgacaagt tagaagaca attttttga agagaccgc 
      ctgtggaat cccgaatgg agaattttt cgagaattg 
      atactggag taccatggc gagcgacga ttgtacctc 
      acgaaaatg ttgcgttaa atgcaaatt ctttactag 
      cgtttggaa gttccgatg aggagtctt ttgatgaca 
      ctttcctac cgaaaatgc gcccgaaac ccaaagttt 
      tcgttaata aaaagaagt tgaaaaggg gttcgggaa 
      gatttttga agaatgcaa cgaaaaacg caaagcaga 
      gtaaactaa agctggatg aaagaagta tgactagtt 
      cagttgcaa gtgaagtac ctcaaacgg cggaccaag 
      gacggatga aaagtaggg caaagggaa aggaaaaag 
      aaagggttg aaactttaa taaaggaac cagaagaga 
      atccaggag ttaaaaata aaaagagac aaagccgaa 
      gtccaacta aatcacaga cgaaacata acgtactag 
      acgctaatc attttcaag aagtgacgt ctaatagtg 
      aactattaa ctgaacgtt ttttgaacg aggaaaata 
      aaaaggttg agagtacac ggaaaggac agaagaaac 
      gggatagga ctgaaagtc cgctcaaaa aattaagaa 
      gatcagata taaacgatt ccaagagtg gcaagcaaa 
      atatgacac aagacgatg gagaaatgg tgtcatatg 
      gtacaaata cagataact ggagaaatg acttatttg 
      gtgaaagtt cgatgaaag aaatggaac atttggaga 
      agggaaaga aatcagggc aaaatttaa agacagaaa 
      aaaagaaac agaacgcaa gtccatcaa aaaaacaaa 
      aagcaatag taaagtgtc ttgcaaata aaaataagg 
      aacacagtg tcactgatg acagtggtt ccgatgaat 
      cgcaaagag tgtcagtta aggcggtcg tacatgcaa 
      gtgctacac aaaaaaata gtaaaaagc cagcaggaa 
      cacaaaaac aacaactaa gaaatgtag acaatatac 
      atattttgg aatcaggat tagacgtaa tgactaata 
      gtgatgaag aaacgtcac gcgaagttg aagcatttt 
      ttgaatcaa ataatcagg atactaatt tcggtgcac 
      cgaatctgg acaatttgc aacagaaac catcactaa 
      ataatagcg ttaataaat cagctgaaa aaagtacag 
      cgaggaaaa cacaaaata aaaagggaa aaaagcaga 
      caaaacgtt ctacaacga agtctaaag cccaaagta 
      caacaggaa ttactgaaa cgacaactg acgacacaa 
      caggatgta tgcaaagga gatcgggaa ggcaacgaa 
      aacaaacaa agattaatt cagctggtt acatcctat 
      gcactgaaa gggacagat attataaag tcgcaacgg 
      cggtaaggc atatatcac aagtaacgc ctgaatgtt 
      catttcgac tttcgggac tacaaaaac ggcgtactc 
      gtataactc aaatgccac caattatgg agtcggaag 
      ttgaaatgg aaggtaata ctcaaaagt cgaaaagga 
      aaactaaga aaccggcta aaaaacaat caagtaaga 
      aacttaaga aagatgaga ccccaaaac gtcaagaaa 
      ctaggactg tttctgaat cggagacaa atctaacca 
      gaagtgatt acattgttg gagaaaatg taattacct 
      ccgattatg ttagtgcag aaaatgttg cacaaaaca 
      ctcaaatac catttgtaa gactagaag aaattactt 
      caaggaaca ttggttctg aaaaacttc ataacaccg 
      tcttttctt caccaccgt tgcaagcac aagagagta 
      tcaagaaaa attctgtta tatctggaa gaatatcta 
      aacggtcta gtaccaaaa ccagcgcga aaaaagtta 
      gggattcct tgaaaaagg tagtcgaga ctcaacgcg 
      actcaagcg gtaatttca tgatctgtc cagcaacta 
      atgagctta ttagataca gtttcaaaa atccggtgt 
      acaaaccga tcgctaact tgcctggtg tcacgtacg 
      ctgtgacgg aagctgtaa aactgggat ggttgaaga 
      taagcggag gaatgtcaa agccattat ttaaaacta 
      agaatcaca ctatgtcgt tcgtagttg tagaaggaa 
      aagcggagg tggggatac aggacaagt gtgatttgt 
      ataaaaaac atgacatat ttattgttc cacaaggag 
      cggaatatt acatagata acaaaaata aaactaagc 
      ccttgctat tggttttct tcaaagacg catatgcgg 
      acaaaccga cgtaataat gatactctt ggcgacaaa 
      tttagttag tagtgtttc ttacattat cgagatcat 
      ttgtttttt agttatact tttcattaa gaatatgaa 
      tattatatc tttaataaa aagtttaca gaact