Status
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Gene information
General information
TC009773 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8017682..8022191
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009773
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01608

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009773 RA
      MTANVFMHS RVSHRNTHC ISFNQSLVE TGVKMSASL 
      SDEQNKLVK KVAESQGFK DYKIDVQAG SLKGDGYLG 
      IINIVKIWD NSKELNLVI KSALSGKQL REQAPVRKA 
      YEREIYMYT TIFPEFEKL SRERSIKNS FREIAKCYG 
      SFLKEYEEC LILENLKEI DYKLWNRKI PMNADHVAL 
      GFATYGKFH GTGLALKYL KPELYQEMS KNLKNIFYS 
      EEETPEEKA KFAESSKLM FGNGFKAVA GNAQATQAL 
      TRFTQQCEK FFGEDVKRI DKYSTILHG DCWCNNMMY 
      KYMDKTNPG KPTEMCFLD WQIAHIGSP IFDLAYFFC 
      ANSGKEVLY DIENFLKIY HENLSATIQ QAGLNPEEV 
      FSFEELQCQ WKHYARFGL FMALFVMRV ILSEVDEVP 
      DLSKCAEEG KDILQSISS SKASNNEEI NKRITDIAI 
      FMDEFGYL
    CDS sequence
    • >TC009773 RA
      atgacggcg aatgtgttc atgcactca cgagttagc 
      caccgaaat acgcattgc atcagtttt aatcaatct 
      ttagtagag acaggcgta aaaatgagt gcttcgtta 
      tctgacgaa caaaataaa ctagtcaag aaagtggca 
      gaaagtcaa ggttttaaa gattataaa atcgatgtc 
      caggcggga tctttaaaa ggcgatggc tatttggga 
      attatcaac atcgtcaaa atctgggat aacagcaag 
      gagcttaat ctggtgatt aaatcagca cttagtggg 
      aaacaacta agagaacaa gctcccgtt aggaaagcc 
      tacgagagg gaaatttat atgtacaca acgattttc 
      cccgaattt gaaaaactt tcacgcgaa aggagcatc 
      aagaacagc ttcagggaa attgcaaag tgctacgga 
      agttttctt aaggaatac gaagaatgc ctaatttta 
      gaaaatctt aaagaaatt gattacaag ctatggaat 
      cgcaaaata ccaatgaat gcagatcat gtcgcttta 
      ggatttgcc acttatggc aaatttcat ggaactgga 
      ctggctttg aaatacctc aaaccagag ttataccaa 
      gaaatgagc aaaaacctg aaaaacatt ttctacagc 
      gaagaggaa acgccagaa gagaaagcg aaatttgcc 
      gagagctcg aaactcatg tttggaaac ggcttcaaa 
      gcagttgcc ggaaacgca caagccacg caagccctc 
      actcggttc acccaacaa tgcgaaaaa ttctttgga 
      gaagacgtc aaaaggatc gataaatat tcgacaatt 
      ctccatggc gattgctgg tgcaacaac atgatgtac 
      aaatatatg gataaaaca aatccgggg aaacccacg 
      gagatgtgt ttcctcgac tggcaaata gcccacatc 
      gggtcccca atttttgac ctcgcgtac ttcttctgt 
      gccaacagt ggaaaggaa gttttatac gacattgag 
      aattttttg aaaatctat cacgaaaat ttatcagcc 
      acaattcaa caggcgggc ttaaacccc gaagaagtt 
      ttttcattt gaagaatta cagtgccag tggaaacat 
      tatgcgaga ttcggtctg tttatggca ctgttcgta 
      atgagagtt attttgagt gaagtcgat gaagttcca 
      gatttgagc aagtgtgcc gaggaggga aaagatatc 
      ttgcagtcg atcagctca tcaaaagct tcgaacaat 
      gaagagatt aataaacgt attacggat attgcgatt 
      tttatggac gaatttggt tatctgtga
    mRNA sequence
    • >TC009773 RA
      aatggtctg tgaaaaatt ccacatgcc gaaaagttg 
      agtaaaacg aattaacac ttcttttga catcgaaaa 
      tgaatagct aggtcccgg aaagaatag attcggaga 
      gtcaagtaa gtattgctc gaaaaggag acgtggtct 
      gtttagtgt cttggaaaa agccggcga aggaaaaag 
      tggcaacaa caatgcatc gtcagagag atatttgtt 
      tgagctgcg gggagtcat ttgaatttc ccgttgata 
      acaaaggca tatgtcctg taaatagta aatgacggc 
      gaatgtgtt catgcactc acgagttag ccaccgaaa 
      tacgcattg catcagttt taatcaatc tttagtaga 
      gacaggcgt aaaaatgag tgcttcgtt atctgacga 
      acaaaataa actagtcaa gaaagtggc agaaagtca 
      aggttttaa agattataa aatcgatgt ccaggcggg 
      atctttaaa aggcgatgg ctatttggg aattatcaa 
      catcgtcaa aatctggga taacagcaa ggagcttaa 
      tctggtgat taaatcagc acttagtgg gaaacaact 
      aagagaaca agctcccgt taggaaagc ctacgagag 
      ggaaattta tatgtacac aacgatttt ccccgaatt 
      tgaaaaact ttcacgcga aaggagcat caagaacag 
      cttcaggga aattgcaaa gtgctacgg aagttttct 
      taaggaata cgaagaatg cctaatttt agaaaatct 
      taaagaaat tgattacaa gctatggaa tcgcaaaat 
      accaatgaa tgcagatca tgtcgcttt aggatttgc 
      cacttatgg caaatttca tggaactgg actggcttt 
      gaaatacct caaaccaga gttatacca agaaatgag 
      caaaaacct gaaaaacat tttctacag cgaagagga 
      aacgccaga agagaaagc gaaatttgc cgagagctc 
      gaaactcat gtttggaaa cggcttcaa agcagttgc 
      cggaaacgc acaagccac gcaagccct cactcggtt 
      cacccaaca atgcgaaaa attctttgg agaagacgt 
      caaaaggat cgataaata ttcgacaat tctccatgg 
      cgattgctg gtgcaacaa catgatgta caaatatat 
      ggataaaac aaatccggg gaaacccac ggagatgtg 
      tttcctcga ctggcaaat agcccacat cgggtcccc 
      aatttttga cctcgcgta cttcttctg tgccaacag 
      tggaaagga agttttata cgacattga gaatttttt 
      gaaaatcta tcacgaaaa tttatcagc cacaattca 
      acaggcggg cttaaaccc cgaagaagt tttttcatt 
      tgaagaatt acagtgcca gtggaaaca ttatgcgag 
      attcggtct gtttatggc actgttcgt aatgagagt 
      tattttgag tgaagtcga tgaagttcc agatttgag 
      caagtgtgc cgaggaggg aaaagatat cttgcagtc 
      gatcagctc atcaaaagc ttcgaacaa tgaagagat 
      taataaacg tattacgga tattgcgat ttttatgga 
      cgaatttgg ttatctgtg attatttta taatgtaac 
      tcaataata atgtgatat acagtattt cgtaaatgt 
      gtgtatttc tttaaaagg attataaat tatgcaacg 
      tttgatgat tatattgta tctacgtat taataaacc 
      gttatgcga ttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY