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Gene information
General information
TC009770 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 1319145..1321306
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009770
    iBeetle screen iB_01607
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: Almost all the empty eggs show 2 or 3 large holelike structures (black).
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009770 RA
      MKRTRSFKE GDKVFAKIK GYPAWPAVI LAKSGKKFN 
      VQFYGTGET GFIKPEDIF YYLKNKEQM VKGSKKKEF 
      KDAVEQIEK AIAEDGTDG DESGVDAGA PAPKRKRSN 
      SEKSSDSVP SKVSSNARN SESEGESNM SESAVDGPN 
      ESRIESENK TFGSADENN AVDEDLPFL PNIKVVSEL 
      NLKNWIIYA DAVKEKPEL YKSRPVDQR HYFENQVFP 
      VLLPSGKYA GLKLHKKWP LHFENEYEC AIYDSDTAS 
      RVLLLKDAV TSGEITPES NPESFLDDF DLTETEIKR 
      DAEMKLINS KYKRVERLR AESSLVELD AKIKNCLGL 
      NKAEPKKAL EFLDSLLEI NMDEIMLKK HSHVVEMIR 
      RLRKYVGNL EEWNLPPEE LETFNNDAQ KVRTKADEI 
      YKKFKSVCK LPAGSVNFW DGFNDIVRK FREDCIHLN 
      NREVFVLCA EPYSRQAII DRLEQEEEL QEADIHRPV 
      DSEIEKVDV Q
    CDS sequence
    • >TC009770 RA
      atgaagcga acccgctca tttaaagaa ggtgacaaa 
      gtgtttgct aaaatcaaa ggctatcca gcttggccg 
      gcagttatt ttagcaaaa agcggtaaa aaattcaat 
      gtgcagttt tacggaact ggagagacg ggctttatt 
      aaacctgaa gacatattc tactatctt aagaataag 
      gaacagatg gtcaaaggg tcgaaaaag aaggagttc 
      aaagacgct gttgagcag attgaaaag gccattgct 
      gaggacgga acagatggt gatgagtct ggtgttgat 
      gcaggtgcc ccagctcct aaaaggaag agaagtaac 
      tcagagaaa tcctcagat tctgttccg tcgaaagtt 
      agcagtaat gctagaaac tctgaaagt gaaggagaa 
      tcaaatatg agtgaaagt gctgtagac ggtcctaac 
      gagtctaga attgaatct gagaacaag acattcggg 
      agtgctgac gaaaataat gcagtggac gaagatttg 
      ccatttttg ccaaacatt aaagtagtt tcggagctt 
      aacttaaaa aattggatt atttatgca gatgcagtt 
      aaggaaaaa ccagagtta tacaaatcc cgccctgtt 
      gaccaacgc cattacttc gaaaaccaa gtatttccg 
      gtattgctc ccttccggc aaatatgca ggccttaaa 
      ctccacaag aagtggccg ctgcacttc gaaaatgaa 
      tacgagtgt gctatttat gacagtgat acagcctcc 
      agagtgctt ctcctcaaa gatgcagtg actagtggc 
      gaaataacc ccagagagc aatcctgaa tcgttcctc 
      gatgatttt gatctcact gaaactgaa ataaagcga 
      gatgctgaa atgaaactg atcaacagc aaatacaaa 
      agggtagag agattgaga gcagagtcc agcttggtt 
      gaacttgac gcaaaaatt aagaattgt ctcggctta 
      aataaagcc gagccaaag aaagcgttg gaattcttg 
      gatagttta cttgagata aatatggac gaaattatg 
      ctaaagaaa cactctcac gttgttgag atgatcagg 
      aggttgaga aaatacgta ggaaattta gaagagtgg 
      aatttacca cctgaggag cttgaaact ttcaacaac 
      gacgcacag aaagtacgg acaaaggca gatgagatt 
      tataagaaa ttcaaatcg gtttgtaaa ttaccggcg 
      gggtctgtt aacttttgg gacggtttc aatgacatt 
      gttaggaaa tttagggag gattgcatc catttgaat 
      aatagggaa gtttttgtt ttgtgtgcc gagccatat 
      tcgcgtcaa gctattatt gaccgactt gagcaggaa 
      gaagaatta caagaggct gatattcac cgtccggtt 
      gatagtgaa attgagaaa gttgacgtt cagtag
    mRNA sequence
    • >TC009770 RA
      ccgctttaa tgttgtgca catgcgttg ttagggggc 
      gctacgttt cccgaagcg tgtgacgcc atttttatt 
      gttgaaaaa aaatccgcc gaaaataca aaattgaaa 
      atcgtcttt ttgcataaa ttcgtgtaa aataacaca 
      aattcacac aacaatgaa gcgaacccg ctcatttaa 
      agaaggtga caaagtgtt tgctaaaat caaaggcta 
      tccagcttg gccggcagt tattttagc aaaaagcgg 
      taaaaaatt caatgtgca gttttacgg aactggaga 
      gacgggctt tattaaacc tgaagacat attctacta 
      tcttaagaa taaggaaca gatggtcaa agggtcgaa 
      aaagaagga gttcaaaga cgctgttga gcagattga 
      aaaggccat tgctgagga cggaacaga tggtgatga 
      gtctggtgt tgatgcagg tgccccagc tcctaaaag 
      gaagagaag taactcaga gaaatcctc agattctgt 
      tccgtcgaa agttagcag taatgctag aaactctga 
      aagtgaagg agaatcaaa tatgagtga aagtgctgt 
      agacggtcc taacgagtc tagaattga atctgagaa 
      caagacatt cgggagtgc tgacgaaaa taatgcagt 
      ggacgaaga tttgccatt tttgccaaa cattaaagt 
      agtttcgga gcttaactt aaaaaattg gattattta 
      tgcagatgc agttaagga aaaaccaga gttatacaa 
      atcccgccc tgttgacca acgccatta cttcgaaaa 
      ccaagtatt tccggtatt gctcccttc cggcaaata 
      tgcaggcct taaactcca caagaagtg gccgctgca 
      cttcgaaaa tgaatacga gtgtgctat ttatgacag 
      tgatacagc ctccagagt gcttctcct caaagatgc 
      agtgactag tggcgaaat aaccccaga gagcaatcc 
      tgaatcgtt cctcgatga ttttgatct cactgaaac 
      tgaaataaa gcgagatgc tgaaatgaa actgatcaa 
      cagcaaata caaaagggt agagagatt gagagcaga 
      gtccagctt ggttgaact tgacgcaaa aattaagaa 
      ttgtctcgg cttaaataa agccgagcc aaagaaagc 
      gttggaatt cttggatag tttacttga gataaatat 
      ggacgaaat tatgctaaa gaaacactc tcacgttgt 
      tgagatgat caggaggtt gagaaaata cgtaggaaa 
      tttagaaga gtggaattt accacctga ggagcttga 
      aactttcaa caacgacgc acagaaagt acggacaaa 
      ggcagatga gatttataa gaaattcaa atcggtttg 
      taaattacc ggcggggtc tgttaactt ttgggacgg 
      tttcaatga cattgttag gaaatttag ggaggattg 
      catccattt gaataatag ggaagtttt tgttttgtg 
      tgccgagcc atattcgcg tcaagctat tattgaccg 
      acttgagca ggaagaaga attacaaga ggctgatat 
      tcaccgtcc ggttgatag tgaaattga gaaagttga 
      cgttcagta gtgttgcaa gtaatacat aaagaagag 
      tttt