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Gene information
General information
TC009770 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 7986492..7988653
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009770
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    iBeetle screen iB_01607

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: Almost all the empty eggs show 2 or 3 large holelike structures (black).
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009770 RA
      MKRTRSFKE GDKVFAKIK GYPAWPAVI LAKSGKKFN 
VQFYGTGET GFIKPEDIF YYLKNKEQM VKGSKKKEF 
KDAVEQIEK AIAEDGTDG DESGVDAGA PAPKRKRSN 
SEKSSDSVP SKVSSNARN SESEGESNM SESAVDGPN 
ESRIESENK TFGSADENN AVDEDLPFL PNIKVVSEL 
NLKNWIIYA DAVKEKPEL YKSRPVDQR HYFENQVFP 
VLLPSGKYA GLKLHKKWP LHFENEYEC AIYDSDTAS 
RVLLLKDAV TSGEITPES NPESFLDDF DLTETEIKR 
DAEMKLINS KYKRVERLR AESSLVELD AKIKNCLGL 
NKAEPKKAL EFLDSLLEI NMDEIMLKK HSHVVEMIR 
RLRKYVGNL EEWNLPPEE LETFNNDAQ KVRTKADEI 
YKKFKSVCK LPAGSVNFW DGFNDIVRK FREDCIHLN 
NREVFVLCA EPYSRQAII DRLEQEEEL QEADIHRPV 
DSEIEKVDV Q
    CDS sequence
    • >TC009770 RA
      atgaagcga acccgctca tttaaagaa ggtgacaaa 
gtgtttgct aaaatcaaa ggctatcca gcttggccg 
gcagttatt ttagcaaaa agcggtaaa aaattcaat 
gtgcagttt tacggaact ggagagacg ggctttatt 
aaacctgaa gacatattc tactatctt aagaataag 
gaacagatg gtcaaaggg tcgaaaaag aaggagttc 
aaagacgct gttgagcag attgaaaag gccattgct 
gaggacgga acagatggt gatgagtct ggtgttgat 
gcaggtgcc ccagctcct aaaaggaag agaagtaac 
tcagagaaa tcctcagat tctgttccg tcgaaagtt 
agcagtaat gctagaaac tctgaaagt gaaggagaa 
tcaaatatg agtgaaagt gctgtagac ggtcctaac 
gagtctaga attgaatct gagaacaag acattcggg 
agtgctgac gaaaataat gcagtggac gaagatttg 
ccatttttg ccaaacatt aaagtagtt tcggagctt 
aacttaaaa aattggatt atttatgca gatgcagtt 
aaggaaaaa ccagagtta tacaaatcc cgccctgtt 
gaccaacgc cattacttc gaaaaccaa gtatttccg 
gtattgctc ccttccggc aaatatgca ggccttaaa 
ctccacaag aagtggccg ctgcacttc gaaaatgaa 
tacgagtgt gctatttat gacagtgat acagcctcc 
agagtgctt ctcctcaaa gatgcagtg actagtggc 
gaaataacc ccagagagc aatcctgaa tcgttcctc 
gatgatttt gatctcact gaaactgaa ataaagcga 
gatgctgaa atgaaactg atcaacagc aaatacaaa 
agggtagag agattgaga gcagagtcc agcttggtt 
gaacttgac gcaaaaatt aagaattgt ctcggctta 
aataaagcc gagccaaag aaagcgttg gaattcttg 
gatagttta cttgagata aatatggac gaaattatg 
ctaaagaaa cactctcac gttgttgag atgatcagg 
aggttgaga aaatacgta ggaaattta gaagagtgg 
aatttacca cctgaggag cttgaaact ttcaacaac 
gacgcacag aaagtacgg acaaaggca gatgagatt 
tataagaaa ttcaaatcg gtttgtaaa ttaccggcg 
gggtctgtt aacttttgg gacggtttc aatgacatt 
gttaggaaa tttagggag gattgcatc catttgaat 
aatagggaa gtttttgtt ttgtgtgcc gagccatat 
tcgcgtcaa gctattatt gaccgactt gagcaggaa 
gaagaatta caagaggct gatattcac cgtccggtt 
gatagtgaa attgagaaa gttgacgtt cagtag
    mRNA sequence
    • >TC009770 RA
      ccgctttaa tgttgtgca catgcgttg ttagggggc 
gctacgttt cccgaagcg tgtgacgcc atttttatt 
gttgaaaaa aaatccgcc gaaaataca aaattgaaa 
atcgtcttt ttgcataaa ttcgtgtaa aataacaca 
aattcacac aacaatgaa gcgaacccg ctcatttaa 
agaaggtga caaagtgtt tgctaaaat caaaggcta 
tccagcttg gccggcagt tattttagc aaaaagcgg 
taaaaaatt caatgtgca gttttacgg aactggaga 
gacgggctt tattaaacc tgaagacat attctacta 
tcttaagaa taaggaaca gatggtcaa agggtcgaa 
aaagaagga gttcaaaga cgctgttga gcagattga 
aaaggccat tgctgagga cggaacaga tggtgatga 
gtctggtgt tgatgcagg tgccccagc tcctaaaag 
gaagagaag taactcaga gaaatcctc agattctgt 
tccgtcgaa agttagcag taatgctag aaactctga 
aagtgaagg agaatcaaa tatgagtga aagtgctgt 
agacggtcc taacgagtc tagaattga atctgagaa 
caagacatt cgggagtgc tgacgaaaa taatgcagt 
ggacgaaga tttgccatt tttgccaaa cattaaagt 
agtttcgga gcttaactt aaaaaattg gattattta 
tgcagatgc agttaagga aaaaccaga gttatacaa 
atcccgccc tgttgacca acgccatta cttcgaaaa 
ccaagtatt tccggtatt gctcccttc cggcaaata 
tgcaggcct taaactcca caagaagtg gccgctgca 
cttcgaaaa tgaatacga gtgtgctat ttatgacag 
tgatacagc ctccagagt gcttctcct caaagatgc 
agtgactag tggcgaaat aaccccaga gagcaatcc 
tgaatcgtt cctcgatga ttttgatct cactgaaac 
tgaaataaa gcgagatgc tgaaatgaa actgatcaa 
cagcaaata caaaagggt agagagatt gagagcaga 
gtccagctt ggttgaact tgacgcaaa aattaagaa 
ttgtctcgg cttaaataa agccgagcc aaagaaagc 
gttggaatt cttggatag tttacttga gataaatat 
ggacgaaat tatgctaaa gaaacactc tcacgttgt 
tgagatgat caggaggtt gagaaaata cgtaggaaa 
tttagaaga gtggaattt accacctga ggagcttga 
aactttcaa caacgacgc acagaaagt acggacaaa 
ggcagatga gatttataa gaaattcaa atcggtttg 
taaattacc ggcggggtc tgttaactt ttgggacgg 
tttcaatga cattgttag gaaatttag ggaggattg 
catccattt gaataatag ggaagtttt tgttttgtg 
tgccgagcc atattcgcg tcaagctat tattgaccg 
acttgagca ggaagaaga attacaaga ggctgatat 
tcaccgtcc ggttgatag tgaaattga gaaagttga 
cgttcagta gtgttgcaa gtaatacat aaagaagag 
tttt
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