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Gene information
General information
TC034790 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 1308866..1317108
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034790
    iBeetle screen iB_01606
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    abdomen cuticle anterior constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034790 RA
      MSTALLAAI AVILCILFR ILNVNSQPQ KPVIWCGDK 
      NFLETILKI SPLIQEPYI PTRLWGFSG HVQTILHSV 
      IGRVKCPWP IGERVFLSL DDGTTLTYD MYQPLDTNF 
      QDDVSIAIC PGIGNTSES VYIRTFVHW AQCHGYRCA 
      VLNHVGALQ SVPVTAPRI FSYGNTNDY HEMVSSLIS 
      RHPNTRIVC VGFSLGGNL VTKYMGELD RERPLHIIG 
      GISICQGYC AIEGTKWLL NWQNFRRFY LYIMTENMK 
      NIILKHRQV LLSEEVKNR YKLNEHEII SAATLPELD 
      EAYTRKVHE FNSITELYS WSSSINYLN NINKPMIFI 
      NAKDDPIVP EPLLEPIKS ITSEKNNML YLELAHGGH 
      LGFYEGGLI YPNPVTWLD RTLVALVGS LALNYNDSI 
      MKSAQI
    CDS sequence
    • >TC034790 RA
      atgtcgaca gccctgtta gccgcaatc gctgttatc 
      ctgtgtatc ctgttcagg atactcaac gtcaacagc 
      caaccacaa aagccagtg atatggtgc ggtgataag 
      aactttctg gaaaccatc ctcaaaatc tcacccctc 
      atccaagaa ccatacatt ccgacgcgg ctatggggc 
      ttcagcggc catgtccag accatcctc cacagcgtc 
      atcggcagg gtaaagtgc ccatggccg ataggcgaa 
      cgagtgttt ctatcactc gacgacggg accacatta 
      acttacgac atgtaccaa cctctagat acaaatttc 
      caagacgac gtatcgatc gccatatgt ccaggcatt 
      ggaaatacc tccgagagt gtttacata cgcactttc 
      gttcattgg gctcaatgt cacggctac agatgcgct 
      gtcttgaac cacgtgggg gcgttgcag agcgtaccg 
      gtcacggcc ccacgcata tttagctac ggtaataca 
      aacgactac catgaaatg gtctcaagt ctgattagc 
      agacaccca aacacccga attgtttgc gtaggcttt 
      agtctaggt ggtaattta gtaaccaaa tatatgggg 
      gaactggat agggagagg cctctgcac ataatcggc 
      ggaatttcg atatgtcaa ggctactgt gctatcgag 
      ggaacaaaa tggttactc aactggcaa aatttccgt 
      cgtttctat ctttacatc atgactgaa aacatgaag 
      aacattatt ttaaagcat cgccaagtc ctactgtct 
      gaggaagtg aagaatcgg tacaagctc aacgagcac 
      gagattatt tccgctgcg actcttccc gaactagac 
      gaagcctac actagaaaa gttcacgaa tttaattcg 
      atcaccgaa ttatattcg tggagttct agcatcaat 
      tacctcaat aacataaac aaaccaatg atttttatt 
      aatgctaaa gacgatccg attgttcca gagccgctg 
      ctagagccc atcaagtcg ataacttct gagaaaaac 
      aatatgctt tatttggag ttggcccac ggcgggcac 
      ttgggcttt tacgagggc ggtttgatc taccccaac 
      cctgtcacc tggttggac cgcacactt gtcgcactg 
      gtcggaagt ctggcgctc aattataac gattcgatc 
      atgaagtcg gcccaaatt tag
    mRNA sequence
    • >TC034790 RA
      tacaacata aaaaaagta gcgaaagag catgtgcac 
      actttcctt cattccttc gagatctcc tagatgatc 
      tcatcgtca agctttaaa aattcgtgt tcccactcc 
      taaaacgtt ccattcttc ttatcattc agtttcggt 
      gcaaatatt gtacgaggt gttcgtttt agaagctca 
      attagggcg ttccttcgc catcatgtc gacagccct 
      gttagccgc aatcgctgt tatcctgtg tatcctgtt 
      caggatact caacgtcaa cagccaacc acaaaagcc 
      agtgatatg gtgcggtga taagaactt tctggaaac 
      catcctcaa aatctcacc cctcatcca agaaccata 
      cattccgac gcggctatg gggcttcag cggccatgt 
      ccagaccat cctccacag cgtcatcgg cagggtaaa 
      gtgcccatg gccgatagg cgaacgagt gtttctatc 
      actcgacga cgggaccac attaactta cgacatgta 
      ccaacctct agatacaaa tttccaaga cgacgtatc 
      gatcgccat atgtccagg cattggaaa tacctccga 
      gagtgttta catacgcac tttcgttca ttgggctca 
      atgtcacgg ctacagatg cgctgtctt gaaccacgt 
      gggggcgtt gcagagcgt accggtcac ggccccacg 
      catatttag ctacggtaa tacaaacga ctaccatga 
      aatggtctc aagtctgat tagcagaca cccaaacac 
      ccgaattgt ttgcgtagg ctttagtct aggtggtaa 
      tttagtaac caaatatat gggggaact ggataggga 
      gaggcctct gcacataat cggcggaat ttcgatatg 
      tcaaggcta ctgtgctat cgagggaac aaaatggtt 
      actcaactg gcaaaattt ccgtcgttt ctatcttta 
      catcatgac tgaaaacat gaagaacat tattttaaa 
      gcatcgcca agtcctact gtctgagga agtgaagaa 
      tcggtacaa gctcaacga gcacgagat tatttccgc 
      tgcgactct tcccgaact agacgaagc ctacactag 
      aaaagttca cgaatttaa ttcgatcac cgaattata 
      ttcgtggag ttctagcat caattacct caataacat 
      aaacaaacc aatgatttt tattaatgc taaagacga 
      tccgattgt tccagagcc gctgctaga gcccatcaa 
      gtcgataac ttctgagaa aaacaatat gctttattt 
      ggagttggc ccacggcgg gcacttggg cttttacga 
      gggcggttt gatctaccc caaccctgt cacctggtt 
      ggaccgcac acttgtcgc actggtcgg aagtctggc 
      gctcaatta taacgattc gatcatgaa gtcggccca 
      aatttaggc accgtatgt gattctttt atcgcccac 
      caaattatt gcatttatg tgtgtgtgt ttaaacgtg 
      tggaattga atacacggc gcatttatt tattgtatt 
      tattgttag atgaacgtg acttaaata caaagttga 
      agcgatggc ttgaatgtt ttatctttt gatagtatt 
      attagttta tgtgacagg tagtgagag gaggtttga 
      gcggggctc aatctcgtt tttggcatt tttggtgct 
      cacgcaccg atcattctc acctgtact taatgtgat 
      acataggat agttttact taattactg atattttta 
      taactttgt acattgatt aacggacac ttgtgattt 
      attgagcgg ctttgttta aatttgttt tatcatttt 
      aatcaattt aaagtctaa aaaattgta ttttttaaa 
      ttttattaa actacacca aaaatggaa ttttatctg 
      taacaactg tattgtaat ttatgtaaa aaatgaata 
      aaacgtatt tcacaaa