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Gene information
General information
TC009690 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4611949..4616282
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009690
    iBeetle screen iB_01598

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009690 RA
      MTAPIVTIE EGQVKGKVA ENYQGGKFY SFLGIPYAK 
      PPVGDRRFK APVPADPWD GVLDATQEG PECPSRHMF 
      FAYQIGSEE NCLNLNVYT RDVENKNGS LKPVMVWIH 
      GGAFLYGSN KSEFFGPDY LMTEDIVLV AINYRLGAF 
      GFLSLEDPS LGVPGNAGM KDMILALKW VQRNIKNFN 
      GDPNNVTIF GESAGSASV HFLYLSPMS KGLFHKAIC 
      QSGSALNCW AQGTSNGRV LANALGYKE TDEKKILEY 
      LKTLPPHRL IRAQHKPLD NFTAHLTRP FCPVVEKPS 
      DEPAFLSQH PIEIIKAGT FNHVPLMMG YVSKEGIFF 
      EAVRKLQPG AKITTDFET EIHHDLKLE KGSDKSKEV 
      ANKIQKFYF GEETPSEKT TDNMYLLKT DTQFLHGIH 
      RSIFHQKQH STEPIYLYR MSLDTSLNY YKTICTAKA 
      LPLLFLCYF LSYKSGEGM KKNFFMKIA KSLPSHRPT 
      GVCHADDLG YLFPSFLSP KLQPDSIEE VSVRRFVKL 
      WTNFAKTGN PTPEDDQLL MNVRWSPVT KEENHFVDI 
      GKELKTGTN PDFERMQFW DTIYSS
    CDS sequence
    • >TC009690 RA
      atgacagct ccaattgtg acaatagag gagggtcaa 
      gttaaaggc aaagtcgcc gaaaactat caagggggc 
      aagttttac agttttttg gggataccg tacgccaag 
      ccgccggtt ggagaccgg agattcaag gcgccggtc 
      ccggccgat ccttgggac ggagtcctt gatgcaaca 
      caagaagga cccgaatgt ccctcccgc cacatgttt 
      ttcgcttac caaatcggg tcggaagaa aactgcctg 
      aatttaaat gtttacacc cgtgatgtt gaaaataaa 
      aatgggtcc cttaaacca gtcatggtt tggatccac 
      ggcggtgcc tttctttat ggctcgaat aagagtgaa 
      tttttcggc cccgattat ctgatgact gaagacatt 
      gtcttggtg gccatcaac tatcgctta ggagccttt 
      gggtttctc agtttggaa gatccgtct ttgggagtt 
      ccgggcaat gccgggatg aaggacatg atcctggcc 
      ctaaagtgg gtgcagaga aacatcaaa aacttcaac 
      ggggacccg aacaatgtc acgattttt ggcgaaagc 
      gccgggtca gcgtccgtc cacttcttg tatctgtcc 
      cccatgtcc aagggcttg ttccacaaa gccatttgt 
      cagagtgga agtgcgctc aattgttgg gcccaaggg 
      accagcaac gggcgcgtt ttggccaac gcgctgggg 
      tacaaagaa accgacgag aaaaaaatt ttggagtac 
      ttaaaaaca ctccctccg catcggtta atccgagcg 
      cagcacaaa cccctcgat aactttacg gcccacctg 
      acgcggcct ttttgccca gttgtggaa aaaccatca 
      gacgaaccg gccttcctc tcccaacac cccatcgaa 
      atcatcaaa gctggcacc ttcaaccat gtaccacta 
      atgatgggt tacgtgtca aaagaaggc atttttttc 
      gaagcggtc cggaagtta caaccgggc gccaaaatc 
      acaaccgat ttcgaaacc gaaatccac cacgacttg 
      aaactcgaa aaagggtcc gacaaatcg aaggaagtt 
      gccaataaa atacaaaaa ttctatttt ggggaagaa 
      accccgtct gagaaaacc acagacaat atgtatctg 
      cttaaaacc gacacccag ttcctgcac ggaatccac 
      aggagtatt ttccaccag aagcagcac agcaccgaa 
      cccatttat ttgtacaga atgtcgctc gacacgtct 
      ttgaattat tacaagacg atttgcacc gccaaagct 
      ttgccgctc ctcttcctt tgctatttc ctgtcgtac 
      aaatcgggc gaaggcatg aagaagaat ttcttcatg 
      aaaatcgcg aaatcgcta ccgagtcat cggccgact 
      ggggtgtgc catgccgat gatcttggc tacctcttc 
      ccttcgttt ttgagtccg aagttgcaa ccagacagt 
      atcgaggaa gtttcagtt cggagattt gttaagttg 
      tggacgaat tttgccaaa actgggaac ccaacgccc 
      gaggacgac caactgctg atgaacgtg cggtggagt 
      ccggtgacc aaagaagaa aaccacttt gtagatata 
      gggaaagag ctgaagact ggaaccaat cccgatttt 
      gaacgcatg cagttctgg gacacgatc tactctagt 
      tga
    mRNA sequence
    • >TC009690 RA
      acagtctga tttcgcgca ccaaatcaa gcaaaatgc 
      tatcgaatg ttgcaaaag gatttcctc cggttctga 
      aatggtgca atagctcca aattttcca ccacgtcca 
      cagtcagtt ccaaactga aacgggcaa ctttgcgag 
      taagtttta taaatagat aacgcacat aatcgccaa 
      ccaatgtta tccacatcc gattccacg tggtttcga 
      cctctgttt cggccaacc agccaagaa tcaccagca 
      acagttata tgttactgc gtgcacttg gtggatctg 
      taagctacc acagtaaaa tgacagctc caattgtga 
      caatagagg agggtcaag ttaaaggca aagtcgccg 
      aaaactatc aagggggca agttttaca gttttttgg 
      ggataccgt acgccaagc cgccggttg gagaccgga 
      gattcaagg cgccggtcc cggccgatc cttgggacg 
      gagtccttg atgcaacac aagaaggac ccgaatgtc 
      cctcccgcc acatgtttt tcgcttacc aaatcgggt 
      cggaagaaa actgcctga atttaaatg tttacaccc 
      gtgatgttg aaaataaaa atgggtccc ttaaaccag 
      tcatggttt ggatccacg gcggtgcct ttctttatg 
      gctcgaata agagtgaat ttttcggcc ccgattatc 
      tgatgactg aagacattg tcttggtgg ccatcaact 
      atcgcttag gagcctttg ggtttctca gtttggaag 
      atccgtctt tgggagttc cgggcaatg ccgggatga 
      aggacatga tcctggccc taaagtggg tgcagagaa 
      acatcaaaa acttcaacg gggacccga acaatgtca 
      cgatttttg gcgaaagcg ccgggtcag cgtccgtcc 
      acttcttgt atctgtccc ccatgtcca agggcttgt 
      tccacaaag ccatttgtc agagtggaa gtgcgctca 
      attgttggg cccaaggga ccagcaacg ggcgcgttt 
      tggccaacg cgctggggt acaaagaaa ccgacgaga 
      aaaaaattt tggagtact taaaaacac tccctccgc 
      atcggttaa tccgagcgc agcacaaac ccctcgata 
      actttacgg cccacctga cgcggcctt tttgcccag 
      ttgtggaaa aaccatcag acgaaccgg ccttcctct 
      cccaacacc ccatcgaaa tcatcaaag ctggcacct 
      tcaaccatg taccactaa tgatgggtt acgtgtcaa 
      aagaaggca tttttttcg aagcggtcc ggaagttac 
      aaccgggcg ccaaaatca caaccgatt tcgaaaccg 
      aaatccacc acgacttga aactcgaaa aagggtccg 
      acaaatcga aggaagttg ccaataaaa tacaaaaat 
      tctattttg gggaagaaa ccccgtctg agaaaacca 
      cagacaata tgtatctgc ttaaaaccg acacccagt 
      tcctgcacg gaatccaca ggagtattt tccaccaga 
      agcagcaca gcaccgaac ccatttatt tgtacagaa 
      tgtcgctcg acacgtctt tgaattatt acaagacga 
      tttgcaccg ccaaagctt tgccgctcc tcttccttt 
      gctatttcc tgtcgtaca aatcgggcg aaggcatga 
      agaagaatt tcttcatga aaatcgcga aatcgctac 
      cgagtcatc ggccgactg gggtgtgcc atgccgatg 
      atcttggct acctcttcc cttcgtttt tgagtccga 
      agttgcaac cagacagta tcgaggaag tttcagttc 
      ggagatttg ttaagttgt ggacgaatt ttgccaaaa 
      ctgggaacc caacgcccg aggacgacc aactgctga 
      tgaacgtgc ggtggagtc cggtgacca aagaagaaa 
      accactttg tagatatag ggaaagagc tgaagactg 
      gaaccaatc ccgattttg aacgcatgc agttctggg 
      acacgatct actctagtt gattttata gtatttaac 
      ttaagttta ttgtaagga aaacgtttt ttaataaat 
      taagtgcac cttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY