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Gene information
General information
TC009661 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3660202..3661374
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009661
    iBeetle screen iB_01595

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009661 RA
      MANMVHCQL WMGSLEPYM TETFIISAF RKMGENPLN 
      VKVMRNKFT GEAAGYCFV HFANDEEAI DAMHKLNGK 
      PIPGTTPVV RFRLNNASN TGRTLLDRE FSVWVGDLS 
      PDVDDYNLY RVFSSKYNT IKTAKVILD SSGFSKGYG 
      FVRFGSEDE MRDSLTTMN GYIGLGTKA LKICNAVPK 
      PKGALTTGT PSATSTTST YTAADYSQF YDPSTYWQN 
      YANWQTGYY EQGDGTGHE AYDMSNVMM EKKEEDLLE 
      LIDHSLPLD VDKINRERI EQDCNLWDA LESSKWLPC 
      EALEVN
    CDS sequence
    • >TC009661 RA
      atggcaaat atggtgcat tgccagttg tggatgggc 
      agtctggag ccctacatg accgaaacg ttcataatt 
      tcggcgttt cgcaaaatg ggggaaaac cccctcaat 
      gtgaaagta atgcgcaac aaattcacc ggcgaagcc 
      gccggttac tgcttcgtg cacttcgcc aacgacgag 
      gaggcaatc gacgccatg cacaagctg aacggcaaa 
      cccattcca gggaccacc cccgttgtg aggttccga 
      ctcaataac gcaagtaac acagggcgg acgcttcta 
      gatcgtgag ttttcagtc tgggtgggc gatttgagc 
      cccgatgtt gacgattat aacttatac agagtgttt 
      tcatccaaa tacaacacg attaaaacg gctaaagtg 
      attttagac agcagtggc ttcagcaaa ggctacggt 
      ttcgtgcgg tttggcagc gaagacgag atgagggac 
      agcttgacc acgatgaac gggtacata gggctgggg 
      accaaggcc ctcaaaatc tgtaacgca gtcccgaag 
      cccaagggc gcgctcacg accgggacc ccgtccgcc 
      accagcacc acctccaca tacacagca gctgattat 
      agccagttt tacgatccc tcgacttat tggcaaaat 
      tacgccaat tggcaaacg ggttactac gaacaaggg 
      gacgggacg ggtcatgaa gcctatgac atgtcgaac 
      gttatgatg gagaagaag gaggaagat ttgcttgaa 
      ttaattgat cattcgctt ccgcttgat gtcgacaaa 
      atcaaccgc gaacgtatc gagcaagac tgcaatctc 
      tgggatgcc ctcgagagt tctaaatgg ttaccgtgt 
      gaggcgcta gaggtcaac tga
    mRNA sequence
    • >TC009661 RA
      tacgttggc actaacttt gtggttaag ttctcattt 
      attaacctt aaagctttg tttgttttt gtgtaaaac 
      agccccaaa atggcaaat atggtgcat tgccagttg 
      tggatgggc agtctggag ccctacatg accgaaacg 
      ttcataatt tcggcgttt cgcaaaatg ggggaaaac 
      cccctcaat gtgaaagta atgcgcaac aaattcacc 
      ggcgaagcc gccggttac tgcttcgtg cacttcgcc 
      aacgacgag gaggcaatc gacgccatg cacaagctg 
      aacggcaaa cccattcca gggaccacc cccgttgtg 
      aggttccga ctcaataac gcaagtaac acagggcgg 
      acgcttcta gatcgtgag ttttcagtc tgggtgggc 
      gatttgagc cccgatgtt gacgattat aacttatac 
      agagtgttt tcatccaaa tacaacacg attaaaacg 
      gctaaagtg attttagac agcagtggc ttcagcaaa 
      ggctacggt ttcgtgcgg tttggcagc gaagacgag 
      atgagggac agcttgacc acgatgaac gggtacata 
      gggctgggg accaaggcc ctcaaaatc tgtaacgca 
      gtcccgaag cccaagggc gcgctcacg accgggacc 
      ccgtccgcc accagcacc acctccaca tacacagca 
      gctgattat agccagttt tacgatccc tcgacttat 
      tggcaaaat tacgccaat tggcaaacg ggttactac 
      gaacaaggg gacgggacg ggtcatgaa gcctatgac 
      atgtcgaac gttatgatg gagaagaag gaggaagat 
      ttgcttgaa ttaattgat cattcgctt ccgcttgat 
      gtcgacaaa atcaaccgc gaacgtatc gagcaagac 
      tgcaatctc tgggatgcc ctcgagagt tctaaatgg 
      ttaccgtgt gaggcgcta gaggtcaac tgacgttaa 
      tttaattgt ttaattaca aacttacaa aaacactat 
      ctaatataa taaaattac ttaaggaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY