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Gene information
General information
TC009651 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid47: 1528268..1529858
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009651
    iBeetle screen iB_01592
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009651 RA
      MNYNEREIK AWLESCFKN LTQIVVTLV GHSEKGDGY 
      MGDIFFVHI DGKNGGTTE SYDLAVKSS KDSDILREF 
      SPMKVFFNN EKYFYESVF PNFVQFQTN RGLKNPFKG 
      VPKCYGTLS GVKDVLVFE NLTKSGYCL WDRKKPMTR 
      DHVELVIRD YAKFHAVSV ALKDQEPQN FEKLVKHYD 
      IFNDLGRDS KNPMLETLQ NAVEEMHDL LQNDLDQLV 
      LQKWRNLKN SIQTFFREM LSYEGFKVI THGDCWNNN 
      FLFFYDEND NPIKVSIID WQISRLASP ILDLSHFIF 
      SCTSENDLD DLTKILEHY HKCFTSFLA QLGSSESLY 
      PKNQFFDDW ANLSKFGIM FSSIVMKVC TAEKEEAPD 
      LAKPPENGQ FTAFQFEVA NKKLLKQRM RAIVSCAAK 
      NKLI
    CDS sequence
    • >TC009651 RA
      atgaattac aacgagcgt gaaatcaaa gcgtggctc 
      gaatcgtgt ttcaaaaac ttgacccaa atcgtagtg 
      acacttgtg gggcactcc gaaaagggc gatggatac 
      atgggcgat attttcttc gtccatatt gatggtaaa 
      aacggcggt accaccgaa agctacgac cttgcagtg 
      aagtccagc aaagacagc gacattctt cgtgaattc 
      tcccccatg aaggttttt ttcaacaac gaaaagtac 
      ttctatgag agtgtgttc ccaaacttt gtccaattc 
      caaacaaat cgggggtta aagaaccca tttaagggt 
      gtccccaaa tgttatggg actttatca ggggttaag 
      gatgtccta gtgtttgaa aatctgacc aaaagtggg 
      tactgttta tgggacagg aagaaaccc atgaccagg 
      gaccatgtc gagctggtg attcgcgac tacgcaaag 
      ttccatgcg gtttcagtg gctttgaaa gaccaggaa 
      ccccaaaac tttgagaag ttggtcaaa cattacgat 
      attttcaat gatctgggg cgtgacagt aaaaacccg 
      atgcttgag actttgcaa aatgctgtt gaagagatg 
      cacgatttg ctccagaat gacttggat cagcttgtt 
      ttgcaaaaa tggaggaac ttgaaaaat agcattcag 
      acgtttttt cgggaaatg ttgagttat gaaggcttc 
      aaagtcatc acacatggc gactgttgg aataataat 
      ttcttgttt ttttacgac gaaaatgat aatccgatc 
      aaagtttca ataatcgac tggcagata agccgatta 
      gcgtcgcca attttagat ttatcccac tttattttt 
      tcatgtact tccgaaaat gatttggac gatttgacc 
      aaaattttg gaacattac cacaaatgt tttacaagc 
      tttctagcc cagttgggc tccagcgaa agtttatat 
      cccaaaaat caatttttt gacgattgg gcgaatttg 
      agcaaattc ggaattatg tttagttcg atcgttatg 
      aaagtttgc acggccgaa aaggaggaa gcaccagat 
      ttggccaaa ccgccagaa aacggccaa ttcacagct 
      ttccagttc gaagtcgcg aataaaaaa ctactgaag 
      cagaggatg agagcgatc gtgagctgt gcagcaaag 
      aacaaactt atttaa
    mRNA sequence
    • >TC009651 RA
      ctggataaa ttcatcaat ttgtgctta tgattcgat 
      ttgtaaaac ttttctgtt tcacttgtt actctgtat 
      cttttttag cattttttg aagcactgt agatagttc 
      gtagcaatt ttgtctgct ttcaagtca gtgacttga 
      attttgttt caagataat cactggtac atttttcca 
      tttttgatc aaactattc aaataattg cttaatgac 
      agtaatttt cttttgttc tctgccttg aataaacac 
      ttgatatta ttatcgcct cgttgatac ttataacct 
      gactttgga aagttttat cccaatctc aaaatgaat 
      tacaacgag cgtgaaatc aaagcgtgg ctcgaatcg 
      tgtttcaaa aacttgacc caaatcgta gtgacactt 
      gtggggcac tccgaaaag ggcgatgga tacatgggc 
      gatattttc ttcgtccat attgatggt aaaaacggc 
      ggtaccacc gaaagctac gaccttgca gtgaagtcc 
      agcaaagac agcgacatt cttcgtgaa ttctccccc 
      atgaaggtt tttttcaac aacgaaaag tacttctat 
      gagagtgtg ttcccaaac tttgtccaa ttccaaaca 
      aatcggggg ttaaagaac ccatttaag ggtgtcccc 
      aaatgttat gggacttta tcaggggtt aaggatgtc 
      ctagtgttt gaaaatctg accaaaagt gggtactgt 
      ttatgggac aggaagaaa cccatgacc agggaccat 
      gtcgagctg gtgattcgc gactacgca aagttccat 
      gcggtttca gtggctttg aaagaccag gaaccccaa 
      aactttgag aagttggtc aaacattac gatattttc 
      aatgatctg gggcgtgac agtaaaaac ccgatgctt 
      gagactttg caaaatgct gttgaagag atgcacgat 
      ttgctccag aatgacttg gatcagctt gttttgcaa 
      aaatggagg aacttgaaa aatagcatt cagacgttt 
      tttcgggaa atgttgagt tatgaaggc ttcaaagtc 
      atcacacat ggcgactgt tggaataat aatttcttg 
      tttttttac gacgaaaat gataatccg atcaaagtt 
      tcaataatc gactggcag ataagccga ttagcgtcg 
      ccaatttta gatttatcc cactttatt ttttcatgt 
      acttccgaa aatgatttg gacgatttg accaaaatt 
      ttggaacat taccacaaa tgttttaca agctttcta 
      gcccagttg ggctccagc gaaagttta tatcccaaa 
      aatcaattt tttgacgat tgggcgaat ttgagcaaa 
      ttcggaatt atgtttagt tcgatcgtt atgaaagtt 
      tgcacggcc gaaaaggag gaagcacca gatttggcc 
      aaaccgcca gaaaacggc caattcaca gctttccag 
      ttcgaagtc gcgaataaa aaactactg aagcagagg 
      atgagagcg atcgtgagc tgtgcagca aagaacaaa 
      cttatttaa aaaataaaa aaattaaaa atta