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Gene information
General information
TC009648 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3598708..3600717
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009648
    iBeetle screen iB_01591

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009648 RA
      MVQEDDRFV IHGPPPLQP LPRLSLGKL VYDSLLANI 
      KKGDAFVDA ASGETISYL DLLKKSCFL AESLLKSGY 
      GRDTIVSIS SENNVQFYI PVIACLYIG AVVAPINHN 
      YTEYETTHS LNICKPRIV FCSKAVAQK FVQLKNRLG 
      FTEKIVIID DDFCDIYGT ETLAHFIKT GLRGFAPLC 
      RFPGVDFDP DKQIAFIMC SSGTTGLPK GVMQTHTNL 
      MVRYMHTID PRYVQKADT FLGILPFFH GFGLVTNFF 
      ALVQGEKIV VIKRFEEKL FLKAVQDYK IPSLWLAPP 
      LVVLLAKSP LVDQYDLSC IREVTSGAA PLSKETEEL 
      VMKRLKIKG IRQGYGLTE ATLGVIMMS VGDIKHGSS 
      GKVATYMKC KIRDPETGK SLGPGKVGE LCFKGPMVM 
      PGYYNNEEA TRNSFTSDG WLLTGDLGY YDQDEYFYI 
      VDRLKELIK YKGFQVAPA ELEAVILSH PKVQDVGVV 
      GLPDESSGE LPVAFVVKK PGANLTEKE IIDFVAGKV 
      SSQKRLRGG VIFVPAIPK NPSGKILRR ELRKMLSEF 
      KSKL
    CDS sequence
    • >TC009648 RA
      atggtacaa gaagacgac cgatttgtg atccatggc 
      ccaccaccc ctccaaccc ttgccacga ctctccctc 
      gggaaacta gtgtacgac agcctgctc gcaaacata 
      aaaaaaggc gacgctttc gtggatgcg gccagtggc 
      gaaaccatt tcgtatctc gatcttttg aaaaaatcg 
      tgttttttg gcagagagc ctcctaaaa agtggatac 
      ggccgcgat acgatcgtc tccatcagc agcgagaat 
      aacgtccag ttttacatc ccggtcatt gcgtgtctt 
      tacatcggg gctgtggtg gcccccatc aaccacaac 
      tacaccgaa tacgagacc acgcactct ctgaatatt 
      tgcaaacct cgaattgtt ttttgctcg aaagcggtg 
      gcgcaaaaa ttcgtccaa cttaaaaat cggttaggt 
      ttcaccgaa aaaattgta ataattgac gatgatttc 
      tgcgatatc tacgggacc gagactttg gcccacttc 
      atcaaaacg gggttgagg ggcttcgcc cctctgtgc 
      cgtttcccg ggggttgat ttcgatcca gacaaacaa 
      atcgccttc attatgtgt tcgtctgga accacgggc 
      cttccgaaa ggcgtcatg caaacgcac accaaccta 
      atggtccga tacatgcac acaatagac ccaagatat 
      gtgcaaaag gcggacact ttcctcgga attttgccg 
      tttttccac ggcttcggt ctcgtgacg aattttttc 
      gccctagtc cagggcgaa aaaatcgtc gttattaaa 
      cggttcgaa gagaaattg ttcctgaag gccgtccaa 
      gactacaaa atcccgagt ttgtggctt gcgccccct 
      ttggtcgtc ttactagcc aagagcccc cttgtcgac 
      cagtacgac ctgtcttgc attagggaa gtgacaagt 
      ggggcggcc ccccttagc aaggaaacc gaagaactg 
      gtcatgaaa cgattgaag ataaagggg atacggcag 
      ggttatggc cttactgag gccactctg ggcgttatc 
      atgatgagt gtcggggac atcaagcac ggatcttcc 
      ggaaaagtc gccacgtac atgaaatgc aaaatccgg 
      gacccggaa accggaaaa tcgctcggg cccggaaaa 
      gtaggagag ttatgtttc aaaggaccc atggtgatg 
      cctggctat tacaacaac gaggaggct accaggaat 
      tcgtttacc agcgatggg tggcttttg acgggagat 
      ttgggttat tatgaccaa gacgagtat ttctatatc 
      gtcgatcgg ttgaaagaa ttgatcaag tacaagggt 
      tttcaggtg gcgccagct gaactcgaa gctgtgatt 
      ttgagtcat ccgaaggtg caagatgtg ggcgttgtg 
      ggcctccct gacgaaagt tccggggaa ttaccggtg 
      gctttcgtc gtcaaaaag ccgggggcg aatttgact 
      gagaaggaa atcatcgat tttgttgcc ggaaaagtc 
      tcatcgcag aagcgcctt cgaggagga gttattttc 
      gtgcctgcg atccccaag aacccctcc ggcaagata 
      ttgcgccgc gaactgaga aaaatgctg tctgaattc 
      aaatcaaaa ttgtaa
    mRNA sequence
    • >TC009648 RA
      taataccat ttaaaaaca tatcgcgcg ctgtacccg 
      gcagaaccc cgcgatttg tcgtcagta accagctag 
      tggtaaaaa tggtacaag aagacgacc gatttgtga 
      tccatggcc caccacccc tccaaccct tgccacgac 
      tctccctcg ggaaactag tgtacgaca gcctgctcg 
      caaacataa aaaaaggcg acgctttcg tggatgcgg 
      ccagtggcg aaaccattt cgtatctcg atcttttga 
      aaaaatcgt gttttttgg cagagagcc tcctaaaaa 
      gtggatacg gccgcgata cgatcgtct ccatcagca 
      gcgagaata acgtccagt tttacatcc cggtcattg 
      cgtgtcttt acatcgggg ctgtggtgg cccccatca 
      accacaact acaccgaat acgagacca cgcactctc 
      tgaatattt gcaaacctc gaattgttt tttgctcga 
      aagcggtgg cgcaaaaat tcgtccaac ttaaaaatc 
      ggttaggtt tcaccgaaa aaattgtaa taattgacg 
      atgatttct gcgatatct acgggaccg agactttgg 
      cccacttca tcaaaacgg ggttgaggg gcttcgccc 
      ctctgtgcc gtttcccgg gggttgatt tcgatccag 
      acaaacaaa tcgccttca ttatgtgtt cgtctggaa 
      ccacgggcc ttccgaaag gcgtcatgc aaacgcaca 
      ccaacctaa tggtccgat acatgcaca caatagacc 
      caagatatg tgcaaaagg cggacactt tcctcggaa 
      ttttgccgt ttttccacg gcttcggtc tcgtgacga 
      attttttcg ccctagtcc agggcgaaa aaatcgtcg 
      ttattaaac ggttcgaag agaaattgt tcctgaagg 
      ccgtccaag actacaaaa tcccgagtt tgtggcttg 
      cgccccctt tggtcgtct tactagcca agagccccc 
      ttgtcgacc agtacgacc tgtcttgca ttagggaag 
      tgacaagtg gggcggccc cccttagca aggaaaccg 
      aagaactgg tcatgaaac gattgaaga taaagggga 
      tacggcagg gttatggcc ttactgagg ccactctgg 
      gcgttatca tgatgagtg tcggggaca tcaagcacg 
      gatcttccg gaaaagtcg ccacgtaca tgaaatgca 
      aaatccggg acccggaaa ccggaaaat cgctcgggc 
      ccggaaaag taggagagt tatgtttca aaggaccca 
      tggtgatgc ctggctatt acaacaacg aggaggcta 
      ccaggaatt cgtttacca gcgatgggt ggcttttga 
      cgggagatt tgggttatt atgaccaag acgagtatt 
      tctatatcg tcgatcggt tgaaagaat tgatcaagt 
      acaagggtt ttcaggtgg cgccagctg aactcgaag 
      ctgtgattt tgagtcatc cgaaggtgc aagatgtgg 
      gcgttgtgg gcctccctg acgaaagtt ccggggaat 
      taccggtgg ctttcgtcg tcaaaaagc cgggggcga 
      atttgactg agaaggaaa tcatcgatt ttgttgccg 
      gaaaagtct catcgcaga agcgccttc gaggaggag 
      ttattttcg tgcctgcga tccccaaga acccctccg 
      gcaagatat tgcgccgcg aactgagaa aaatgctgt 
      ctgaattca aatcaaaat tgtaatttt ctaccaata 
      aattaaaat tctcacc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY