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Gene information
General information
TC009644 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3574128..3575739
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009644
    iBeetle screen iB_01589

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009644 RA
      MSRLCVLFF LAFFNFGIL ANPSVVIIG AGPSGIAAA 
      TKLLQNGIH DIKILEAED RIGGRINSV HFSDGIVDL 
      GAQYCHGEK GNVAYNLAK DLDVLEPGL RSLQNNVYY 
      SNGSRLDPD LIEELRQVY LKYDQNENY DTKGKSLGD 
      VFIQKYNTT LFPKHLNSE TESLLSEGL RFLEGYVLI 
      HEGAFSWFN VSADCDYVQ CEGNQALTW KGGYKTVLK 
      IMMDGLPID EKIRLKTRV EQINWDKNT VTVLASNNR 
      TYSADYAIF TPSVGVLKR HKLFTPNLP IAKQKSVEA 
      TGFEGVMKV FLHFPQKWW GDSDQAFAF FWSQKDLKL 
      IWDKPWVTQ VRYILKVPH NSNVWVAWI TGDLVPEIE 
      KLPLEVFKI GVKFVLERF LNGKYHVTE IGDVLRSNW 
      CTNPNFGGT YSFTRVGFY NKGFSHQEK LAEPLLNEG 
      KPVVLFAGE AKCTSCALS DRSWGY
    CDS sequence
    • >TC009644 RA
      atgtcgcgt ttgtgtgtt ttatttttt cttgctttc 
      ttcaatttt ggtattttg gcaaatcct tccgttgtt 
      ataatcggc gctgggcct tcaggaata gcggcagca 
      acaaaactt ctacaaaac ggaatccac gacattaaa 
      atcctcgaa gctgaggat cgaattggt ggccgaata 
      aattccgtc cactttagt gatggaatt gttgatttg 
      ggggcacaa tactgccat ggagagaaa ggcaatgtt 
      gcctataat ctagcaaag gatttggac gtccttgag 
      ccaggctta aggtccctt caaaacaat gtctactac 
      tcgaacggc tcacgactc gaccccgac ctgatcgaa 
      gaattgaga caagtgtat ttgaaatat gaccaaaac 
      gaaaactat gacactaaa ggaaaatct ttgggagac 
      gtgtttatc caaaaatac aatacgaca ctgttcccc 
      aaacattta aacagcgaa actgagtca ttattgagc 
      gaagggttg cggttcctg gaaggttac gttctaatc 
      cacgaaggt gccttttcc tggtttaat gtgtcggct 
      gactgtgac tatgtccaa tgcgagggg aaccaagcc 
      ctgacctgg aaagggggc tacaagacc gtcctcaaa 
      atcatgatg gacggctta ccaatcgac gaaaaaatt 
      cgtctcaaa accagagtc gaacaaatc aattgggac 
      aaaaacaca gtcacagtt ttagcatca aataaccgg 
      acgtattcg gccgattat gcaattttc accccatct 
      gttggtgtt ttaaaacgc cacaaatta ttcactcca 
      aatctacca atcgccaag caaaaatca gtcgaagcc 
      accggtttt gaaggcgta atgaaagtc ttcctgcac 
      ttccctcaa aaatggtgg ggggactcc gaccaagcg 
      tttgcattt ttctggagc cagaaggac ctgaagctg 
      atatgggac aaaccatgg gtgacccaa gtccgctac 
      atcctcaaa gtcccccat aattccaac gtatgggtg 
      gcctggatc acgggcgat ttggttcca gaaattgaa 
      aaactacca ttggaggtt ttcaagatt ggggttaaa 
      ttcgtcttg gagcggttt ttgaacggg aaataccac 
      gtgaccgag attggggat gttcttaga tcgaattgg 
      tgcacaaac cccaatttt ggcggcact tattcattc 
      acgagggtt gggttttac aataagggg ttttcgcat 
      caggagaaa ctagctgag cctttgcta aatgaggga 
      aagccggtg gttttgttt gcaggggag gcaaagtgc 
      acatcctgt gcactttca gaccgttca tggggctat 
      tga
    mRNA sequence
    • >TC009644 RA
      aaataaaca tttcaccag ttttgataa atcattaga 
      ttaaaaatg tcgcgtttg tgtgtttta ttttttctt 
      gctttcttc aattttggt attttggca aatccttcc 
      gttgttata atcggcgct gggccttca ggaatagcg 
      gcagcaaca aaacttcta caaaacgga atccacgac 
      attaaaatc ctcgaagct gaggatcga attggtggc 
      cgaataaat tccgtccac tttagtgat ggaattgtt 
      gatttgggg gcacaatac tgccatgga gagaaaggc 
      aatgttgcc tataatcta gcaaaggat ttggacgtc 
      cttgagcca ggcttaagg tcccttcaa aacaatgtc 
      tactactcg aacggctca cgactcgac cccgacctg 
      atcgaagaa ttgagacaa gtgtatttg aaatatgac 
      caaaacgaa aactatgac actaaagga aaatctttg 
      ggagacgtg tttatccaa aaatacaat acgacactg 
      ttccccaaa catttaaac agcgaaact gagtcatta 
      ttgagcgaa gggttgcgg ttcctggaa ggttacgtt 
      ctaatccac gaaggtgcc ttttcctgg tttaatgtg 
      tcggctgac tgtgactat gtccaatgc gaggggaac 
      caagccctg acctggaaa gggggctac aagaccgtc 
      ctcaaaatc atgatggac ggcttacca atcgacgaa 
      aaaattcgt ctcaaaacc agagtcgaa caaatcaat 
      tgggacaaa aacacagtc acagtttta gcatcaaat 
      aaccggacg tattcggcc gattatgca attttcacc 
      ccatctgtt ggtgtttta aaacgccac aaattattc 
      actccaaat ctaccaatc gccaagcaa aaatcagtc 
      gaagccacc ggttttgaa ggcgtaatg aaagtcttc 
      ctgcacttc cctcaaaaa tggtggggg gactccgac 
      caagcgttt gcatttttc tggagccag aaggacctg 
      aagctgata tgggacaaa ccatgggtg acccaagtc 
      cgctacatc ctcaaagtc ccccataat tccaacgta 
      tgggtggcc tggatcacg ggcgatttg gttccagaa 
      attgaaaaa ctaccattg gaggttttc aagattggg 
      gttaaattc gtcttggag cggtttttg aacgggaaa 
      taccacgtg accgagatt ggggatgtt cttagatcg 
      aattggtgc acaaacccc aattttggc ggcacttat 
      tcattcacg agggttggg ttttacaat aaggggttt 
      tcgcatcag gagaaacta gctgagcct ttgctaaat 
      gagggaaag ccggtggtt ttgtttgca ggggaggca 
      aagtgcaca tcctgtgca ctttcagac cgttcatgg 
      ggctattga gacggggta tcgggaggc ggaaaggat 
      aattcgcat tcacaacca tacaaataa aatttaatt 
      gttcaaata aaaaactat atttcctaa aacattaaa 
      ttattttgt aagtttttt ttagaaatc ttaaataaa 
      tctatttct agagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY