Status
Please wait ...
Gene information
General information
TC033596 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid47: 1482210..1486433
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033596
    iBeetle screen iB_01588
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    head mostlyremaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    metathorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033596 RA
      MHFQFLAEN RIGGRINTV KFGDGLVEL GAEYCHGEV 
      GNIVKELVN GYDLLEPNF NYLNGEIYY SNGSKLDHG 
      FVREMQDLI LSENKEENY DTRGKSIGE VFMHKYNST 
      LVEKYKSEE NKLKLLKEG LHFAERSIL ISEGSFSWF 
      DASADSDWL ECPGNQTLV WKGVGYKTV LEILMKSYP 
      NPDEKLPLD DKLFLNSKV TKINWGEKP IKVHTSDKV 
      YSADYVIFT PSIGVLKAG SDLFTPSLP PKKHKAIDS 
      IGFAGVVKL FLRFPVKWW DDNDKYFVF FWSDDDLKS 
      ENFPEGPQK NGKSWVTQL LDLSRVGHN TNVWMIWIS 
      GEMVPEIEQ LPIETLKKG VNFTLEKFL GKDYNITEI 
      GEVLRSGWV TNENFRGTY SFTRNGLYL KEVSYQNDL 
      AEPLEGLFF AGEATNPVH FATVHGAIE IGAGASGIA 
      ATAKLLDNN VTNVTILEA ENLMGGRVW TVSFGNGLV 
      ELGAEYCTG QKGNFVYEV AQHLLEPSE ELFAHNVYY 
      SNGTKLDVA LMNELGKSL EDLFYHRLN STVVQKYKN 
      DEEKLRIVL KEFPRHAET YIAPTEGAF TWSDISVDK 
      DYQECEGHS MVWKKVGYK TIFDFLLKK HSIEDKLHL 
      NSKVTQINW NQSELVTVY TSDNKSYSA DFVIFTPSV 
      GVLKHEKTL FNPPLPSSK QQSIKATGF AGVMKAFVQ 
      FRTKWWLDN DTEFSFLWS ENDLKNTSF TSGPSKNGI 
      PWVSQLTDF LKVPHNPKV WVWWISGDL IPELEKLPP 
      ETMKAGFVY VLDKFLGKN YNVSEIEAI VTPKWYTTE 
      HFRGVYSFT KTGFYEKGF SHQEKLGEP LVGVSGKPA 
      VLFAGEATN RVHYATVHG AIETGFREA GRIIQTL
    CDS sequence
    • >TC033596 RA
      atgcatttt caattctta gccgaaaat cgcattgga 
      gggcgaatt aacaccgtc aagttcgga gatgggttg 
      gttgagcta ggggctgag tactgtcat ggggaggtg 
      ggaaacatc gtcaaggag ctggtcaat ggttatgat 
      ttgcttgag cccaatttt aattacctc aatggggag 
      atttactac tcgaatggg tctaaactg gaccatggg 
      ttcgtgcgg gagatgcag gacttgatt ttgagcgaa 
      aacaaggag gagaattac gatactcgg gggaaatcg 
      attggggag gttttcatg cacaaatac aattcaacc 
      cttgtcgaa aaatacaaa agcgaagaa aacaaattg 
      aaattgctc aaagaaggg ttgcatttt gccgaacgt 
      tcaatcctg atttctgaa ggttccttc tcatggttt 
      gacgcttcg gccgactcc gattggttg gagtgtccc 
      gggaaccaa acattggtc tggaaaggg gtgggttac 
      aaaaccgtc cttgaaata ctgatgaaa agttatccc 
      aacccggac gaaaaactg cccttagac gataaattg 
      ttcctcaat tctaaagta acgaaaatc aactggggg 
      gaaaaaccc atcaaagtc cacacttct gacaaggtt 
      tactccgcc gattatgtg attttcact ccttcaatt 
      ggggttttg aaagccggg agtgactta ttcacccct 
      tctctcccg cctaagaaa cacaaagcc atcgattcg 
      atcggtttt gctggagtt gttaagctt tttttgcgg 
      tttccggtc aagtggtgg gatgacaat gacaagtat 
      ttcgtcttt ttctggagt gatgacgac ttgaaaagt 
      gagaatttc ccggaaggt ccccagaaa aacggaaaa 
      tcgtgggtt acccagctg ttggacttg tcccgagtt 
      gggcacaac accaacgtt tggatgatc tggatttcg 
      ggagagatg gtccccgag attgaacaa ttgcccatt 
      gagacgttg aaaaagggg gtgaatttc acccttgag 
      aagttcctc ggcaaggat tacaacatt actgaaata 
      ggagaagtt ttgaggtcg ggttgggtc actaatgag 
      aatttccgg gggacgtat tcgttcacc cggaacggg 
      ttgtacctg aaagaggtg tcgtatcag aacgacttg 
      gcggagccg ctcgaagga ctgtttttc gctggggag 
      gctacaaat ccggtgcat tttgcgaca gtgcatggg 
      gctatcgag attggagcg ggggcttcc ggaattgcc 
      gcaactgcg aaacttttg gataataac gttacaaat 
      gtgacaatt ctggaggcg gaaaattta atgggggga 
      agggtctgg actgttagt tttggaaat ggtttggtg 
      gaactaggg gctgaatat tgtaccgga caaaagggg 
      aattttgtt tacgaagtt gcccaacat ttattagaa 
      ccaagtgaa gaattattt gcacataac gtctactat 
      tcaaacggt actaagctc gatgttgcc ctaatgaat 
      gaattggga aagtctttg gaagattta ttctaccac 
      agactgaac tcgactgtg gttcaaaaa tacaaaaat 
      gatgaggag aaattaagg attgtgctg aaagagttt 
      ccacgacat gctgaaacg tacattgcg ccaactgaa 
      ggtgccttc acttggtcg gatatatct gtagataag 
      gactaccaa gagtgtgaa ggacactca atggtttgg 
      aaaaaagtg ggctacaaa acgattttt gattttctc 
      ttgaaaaaa cactcaatc gaagacaaa ctccacttg 
      aattcaaaa gtgactcaa atcaattgg aaccaaagc 
      gaacttgtc acagtctac acttctgat aacaaatcg 
      tactctgcc gattttgtc atcttcacc ccatccgtt 
      ggagtctta aaacacgag aaaacttta tttaatccg 
      cctctcccg tcttcaaaa cagcaaagt atcaaagcc 
      accggtttt gctggagtc atgaaagca ttcgtccag 
      ttccgaaca aaatggtgg cttgacaac gacaccgaa 
      ttttccttc ctctggagc gagaacgac ttgaaaaac 
      acttcgttc acatcagga cccagcaaa aacgggatt 
      ccttgggtg tcccaattg accgatttc ctgaaagtt 
      ccgcataat cccaaagtg tgggtttgg tggatcagc 
      ggagactta atccccgaa cttgagaaa cttcctcca 
      gaaacaatg aaagcgggt tttgtctac gtcttggac 
      aagtttctc gggaaaaat tacaacgtt tctgagatt 
      gaggcaatt gtgacgccg aaatggtac acaactgaa 
      catttccgg ggggtttac tcgttcacc aaaaccgga 
      ttttacgaa aaagggttc tcgcaccag gaaaaactg 
      ggagagcct ttggtggga gtttcggga aagccggcg 
      gtgttgttt gcaggagaa gccacaaat cgggttcat 
      tatgcgacc gttcatggg gccattgag acgggattt 
      cgggaagct ggacgaatt attcaaact ctttga
    mRNA sequence
    • >TC033596 RA
      gctaacttt gggtgtcgt ttgtggctt accacgaca 
      tgcattttc aattcttag ccgaaaatc gcattggag 
      ggcgaatta acaccgtca agttcggag atgggttgg 
      ttgagctag gggctgagt actgtcatg gggaggtgg 
      gaaacatcg tcaaggagc tggtcaatg gttatgatt 
      tgcttgagc ccaatttta attacctca atggggaga 
      tttactact cgaatgggt ctaaactgg accatgggt 
      tcgtgcggg agatgcagg acttgattt tgagcgaaa 
      acaaggagg agaattacg atactcggg ggaaatcga 
      ttggggagg ttttcatgc acaaataca attcaaccc 
      ttgtcgaaa aatacaaaa gcgaagaaa acaaattga 
      aattgctca aagaagggt tgcattttg ccgaacgtt 
      caatcctga tttctgaag gttccttct catggtttg 
      acgcttcgg ccgactccg attggttgg agtgtcccg 
      ggaaccaaa cattggtct ggaaagggg tgggttaca 
      aaaccgtcc ttgaaatac tgatgaaaa gttatccca 
      acccggacg aaaaactgc ccttagacg ataaattgt 
      tcctcaatt ctaaagtaa cgaaaatca actgggggg 
      aaaaaccca tcaaagtcc acacttctg acaaggttt 
      actccgccg attatgtga ttttcactc cttcaattg 
      gggttttga aagccggga gtgacttat tcacccctt 
      ctctcccgc ctaagaaac acaaagcca tcgattcga 
      tcggttttg ctggagttg ttaagcttt ttttgcggt 
      ttccggtca agtggtggg atgacaatg acaagtatt 
      tcgtctttt tctggagtg atgacgact tgaaaagtg 
      agaatttcc cggaaggtc cccagaaaa acggaaaat 
      cgtgggtta cccagctgt tggacttgt cccgagttg 
      ggcacaaca ccaacgttt ggatgatct ggatttcgg 
      gagagatgg tccccgaga ttgaacaat tgcccattg 
      agacgttga aaaaggggg tgaatttca cccttgaga 
      agttcctcg gcaaggatt acaacatta ctgaaatag 
      gagaagttt tgaggtcgg gttgggtca ctaatgaga 
      atttccggg ggacgtatt cgttcaccc ggaacgggt 
      tgtacctga aagaggtgt cgtatcaga acgacttgg 
      cggagccgc tcgaaggac tgtttttcg ctggggagg 
      ctacaaatc cggtgcatt ttgcgacag tgcatgggg 
      ctatcgaga ttggagcgg gggcttccg gaattgccg 
      caactgcga aacttttgg ataataacg ttacaaatg 
      tgacaattc tggaggcgg aaaatttaa tggggggaa 
      gggtctgga ctgttagtt ttggaaatg gtttggtgg 
      aactagggg ctgaatatt gtaccggac aaaagggga 
      attttgttt acgaagttg cccaacatt tattagaac 
      caagtgaag aattatttg cacataacg tctactatt 
      caaacggta ctaagctcg atgttgccc taatgaatg 
      aattgggaa agtctttgg aagatttat tctaccaca 
      gactgaact cgactgtgg ttcaaaaat acaaaaatg 
      atgaggaga aattaagga ttgtgctga aagagtttc 
      cacgacatg ctgaaacgt acattgcgc caactgaag 
      gtgccttca cttggtcgg atatatctg tagataagg 
      actaccaag agtgtgaag gacactcaa tggtttgga 
      aaaaagtgg gctacaaaa cgatttttg attttctct 
      tgaaaaaac actcaatcg aagacaaac tccacttga 
      attcaaaag tgactcaaa tcaattgga accaaagcg 
      aacttgtca cagtctaca cttctgata acaaatcgt 
      actctgccg attttgtca tcttcaccc catccgttg 
      gagtcttaa aacacgaga aaactttat ttaatccgc 
      ctctcccgt cttcaaaac agcaaagta tcaaagcca 
      ccggttttg ctggagtca tgaaagcat tcgtccagt 
      tccgaacaa aatggtggc ttgacaacg acaccgaat 
      tttccttcc tctggagcg agaacgact tgaaaaaca 
      cttcgttca catcaggac ccagcaaaa acgggattc 
      cttgggtgt cccaattga ccgatttcc tgaaagttc 
      cgcataatc ccaaagtgt gggtttggt ggatcagcg 
      gagacttaa tccccgaac ttgagaaac ttcctccag 
      aaacaatga aagcgggtt ttgtctacg tcttggaca 
      agtttctcg ggaaaaatt acaacgttt ctgagattg 
      aggcaattg tgacgccga aatggtaca caactgaac 
      atttccggg gggtttact cgttcacca aaaccggat 
      tttacgaaa aagggttct cgcaccagg aaaaactgg 
      gagagcctt tggtgggag tttcgggaa agccggcgg 
      tgttgtttg caggagaag ccacaaatc gggttcatt 
      atgcgaccg ttcatgggg ccattgaga cgggatttc 
      gggaagctg gacgaatta ttcaaactc tttgacttt 
      gttttttat taatcagca aaaactaca tgacatgac 
      ctttattac atcacactg aaattttgt aacaaattc 
      ataaacatt tcatcagtt ttgataaat ccttgagcg 
      gaagatttg attatttta tatttttta ggagaacct 
      atatttaga atggaattt tctaaattt acttgacat 
      ataattggt aaaacacaa attaatgct gaataattg 
      aaaaagttg tttatcaga caaatcttt tttttttct 
      cattgatga gcaaatacc gcagattca tggaggccc 
      ggagactaa atctgccta agaaataaa aagtattac 
      tgtctagtt ataaataat tagatattt tttgtgtct 
      ttttcgcaa aaaaatgcc tattttaat gtctccttc 
      cataatatt ccttcaata atttttaac acagtttaa 
      cactaaaaa aaaacatca gctaatgca ttcgcgccc 
      cgaatctag tacaccaaa tttccgcaa ttttttttt 
      gattcccta atgattttt tctgatttt tctatatct 
      ttcagtttt agtatttat tgaacagac ggtataaaa 
      aaaattttg ttcagtcta cgtcaagga tttctccac 
      tttgtgatt tgtttgagt ttgctttct tgatgtaat 
      gtaaaattt gctatttga ataataaat ttctggtcc 
      atatagaga ggagaaact ccagactta agtaaattg 
      ctatcagat caatacgtt atttattta gttccaatt 
      tttgataat aacacctaa aataaaaga tctagctca 
      aaattatct ttaatttca cgatcttag caaagacga 
      aagcagtgt tttttagat agcagttag tttggaaat 
      tattttttc taattagtt ttttgtctt gcagcgtaa 
      aaatttaaa aagaaacta aagcatcaa attgaaa