Status
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Gene information
General information
TC033596 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3564110..3568333
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033596
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01588

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    head mostlyremaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    metathorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033596 RA
      MHFQFLAEN RIGGRINTV KFGDGLVEL GAEYCHGEV 
GNIVKELVN GYDLLEPNF NYLNGEIYY SNGSKLDHG 
FVREMQDLI LSENKEENY DTRGKSIGE VFMHKYNST 
LVEKYKSEE NKLKLLKEG LHFAERSIL ISEGSFSWF 
DASADSDWL ECPGNQTLV WKGVGYKTV LEILMKSYP 
NPDEKLPLD DKLFLNSKV TKINWGEKP IKVHTSDKV 
YSADYVIFT PSIGVLKAG SDLFTPSLP PKKHKAIDS 
IGFAGVVKL FLRFPVKWW DDNDKYFVF FWSDDDLKS 
ENFPEGPQK NGKSWVTQL LDLSRVGHN TNVWMIWIS 
GEMVPEIEQ LPIETLKKG VNFTLEKFL GKDYNITEI 
GEVLRSGWV TNENFRGTY SFTRNGLYL KEVSYQNDL 
AEPLEGLFF AGEATNPVH FATVHGAIE IGAGASGIA 
ATAKLLDNN VTNVTILEA ENLMGGRVW TVSFGNGLV 
ELGAEYCTG QKGNFVYEV AQHLLEPSE ELFAHNVYY 
SNGTKLDVA LMNELGKSL EDLFYHRLN STVVQKYKN 
DEEKLRIVL KEFPRHAET YIAPTEGAF TWSDISVDK 
DYQECEGHS MVWKKVGYK TIFDFLLKK HSIEDKLHL 
NSKVTQINW NQSELVTVY TSDNKSYSA DFVIFTPSV 
GVLKHEKTL FNPPLPSSK QQSIKATGF AGVMKAFVQ 
FRTKWWLDN DTEFSFLWS ENDLKNTSF TSGPSKNGI 
PWVSQLTDF LKVPHNPKV WVWWISGDL IPELEKLPP 
ETMKAGFVY VLDKFLGKN YNVSEIEAI VTPKWYTTE 
HFRGVYSFT KTGFYEKGF SHQEKLGEP LVGVSGKPA 
VLFAGEATN RVHYATVHG AIETGFREA GRIIQTL
    CDS sequence
    • >TC033596 RA
      atgcatttt caattctta gccgaaaat cgcattgga 
gggcgaatt aacaccgtc aagttcgga gatgggttg 
gttgagcta ggggctgag tactgtcat ggggaggtg 
ggaaacatc gtcaaggag ctggtcaat ggttatgat 
ttgcttgag cccaatttt aattacctc aatggggag 
atttactac tcgaatggg tctaaactg gaccatggg 
ttcgtgcgg gagatgcag gacttgatt ttgagcgaa 
aacaaggag gagaattac gatactcgg gggaaatcg 
attggggag gttttcatg cacaaatac aattcaacc 
cttgtcgaa aaatacaaa agcgaagaa aacaaattg 
aaattgctc aaagaaggg ttgcatttt gccgaacgt 
tcaatcctg atttctgaa ggttccttc tcatggttt 
gacgcttcg gccgactcc gattggttg gagtgtccc 
gggaaccaa acattggtc tggaaaggg gtgggttac 
aaaaccgtc cttgaaata ctgatgaaa agttatccc 
aacccggac gaaaaactg cccttagac gataaattg 
ttcctcaat tctaaagta acgaaaatc aactggggg 
gaaaaaccc atcaaagtc cacacttct gacaaggtt 
tactccgcc gattatgtg attttcact ccttcaatt 
ggggttttg aaagccggg agtgactta ttcacccct 
tctctcccg cctaagaaa cacaaagcc atcgattcg 
atcggtttt gctggagtt gttaagctt tttttgcgg 
tttccggtc aagtggtgg gatgacaat gacaagtat 
ttcgtcttt ttctggagt gatgacgac ttgaaaagt 
gagaatttc ccggaaggt ccccagaaa aacggaaaa 
tcgtgggtt acccagctg ttggacttg tcccgagtt 
gggcacaac accaacgtt tggatgatc tggatttcg 
ggagagatg gtccccgag attgaacaa ttgcccatt 
gagacgttg aaaaagggg gtgaatttc acccttgag 
aagttcctc ggcaaggat tacaacatt actgaaata 
ggagaagtt ttgaggtcg ggttgggtc actaatgag 
aatttccgg gggacgtat tcgttcacc cggaacggg 
ttgtacctg aaagaggtg tcgtatcag aacgacttg 
gcggagccg ctcgaagga ctgtttttc gctggggag 
gctacaaat ccggtgcat tttgcgaca gtgcatggg 
gctatcgag attggagcg ggggcttcc ggaattgcc 
gcaactgcg aaacttttg gataataac gttacaaat 
gtgacaatt ctggaggcg gaaaattta atgggggga 
agggtctgg actgttagt tttggaaat ggtttggtg 
gaactaggg gctgaatat tgtaccgga caaaagggg 
aattttgtt tacgaagtt gcccaacat ttattagaa 
ccaagtgaa gaattattt gcacataac gtctactat 
tcaaacggt actaagctc gatgttgcc ctaatgaat 
gaattggga aagtctttg gaagattta ttctaccac 
agactgaac tcgactgtg gttcaaaaa tacaaaaat 
gatgaggag aaattaagg attgtgctg aaagagttt 
ccacgacat gctgaaacg tacattgcg ccaactgaa 
ggtgccttc acttggtcg gatatatct gtagataag 
gactaccaa gagtgtgaa ggacactca atggtttgg 
aaaaaagtg ggctacaaa acgattttt gattttctc 
ttgaaaaaa cactcaatc gaagacaaa ctccacttg 
aattcaaaa gtgactcaa atcaattgg aaccaaagc 
gaacttgtc acagtctac acttctgat aacaaatcg 
tactctgcc gattttgtc atcttcacc ccatccgtt 
ggagtctta aaacacgag aaaacttta tttaatccg 
cctctcccg tcttcaaaa cagcaaagt atcaaagcc 
accggtttt gctggagtc atgaaagca ttcgtccag 
ttccgaaca aaatggtgg cttgacaac gacaccgaa 
ttttccttc ctctggagc gagaacgac ttgaaaaac 
acttcgttc acatcagga cccagcaaa aacgggatt 
ccttgggtg tcccaattg accgatttc ctgaaagtt 
ccgcataat cccaaagtg tgggtttgg tggatcagc 
ggagactta atccccgaa cttgagaaa cttcctcca 
gaaacaatg aaagcgggt tttgtctac gtcttggac 
aagtttctc gggaaaaat tacaacgtt tctgagatt 
gaggcaatt gtgacgccg aaatggtac acaactgaa 
catttccgg ggggtttac tcgttcacc aaaaccgga 
ttttacgaa aaagggttc tcgcaccag gaaaaactg 
ggagagcct ttggtggga gtttcggga aagccggcg 
gtgttgttt gcaggagaa gccacaaat cgggttcat 
tatgcgacc gttcatggg gccattgag acgggattt 
cgggaagct ggacgaatt attcaaact ctttga
    mRNA sequence
    • >TC033596 RA
      gctaacttt gggtgtcgt ttgtggctt accacgaca 
tgcattttc aattcttag ccgaaaatc gcattggag 
ggcgaatta acaccgtca agttcggag atgggttgg 
ttgagctag gggctgagt actgtcatg gggaggtgg 
gaaacatcg tcaaggagc tggtcaatg gttatgatt 
tgcttgagc ccaatttta attacctca atggggaga 
tttactact cgaatgggt ctaaactgg accatgggt 
tcgtgcggg agatgcagg acttgattt tgagcgaaa 
acaaggagg agaattacg atactcggg ggaaatcga 
ttggggagg ttttcatgc acaaataca attcaaccc 
ttgtcgaaa aatacaaaa gcgaagaaa acaaattga 
aattgctca aagaagggt tgcattttg ccgaacgtt 
caatcctga tttctgaag gttccttct catggtttg 
acgcttcgg ccgactccg attggttgg agtgtcccg 
ggaaccaaa cattggtct ggaaagggg tgggttaca 
aaaccgtcc ttgaaatac tgatgaaaa gttatccca 
acccggacg aaaaactgc ccttagacg ataaattgt 
tcctcaatt ctaaagtaa cgaaaatca actgggggg 
aaaaaccca tcaaagtcc acacttctg acaaggttt 
actccgccg attatgtga ttttcactc cttcaattg 
gggttttga aagccggga gtgacttat tcacccctt 
ctctcccgc ctaagaaac acaaagcca tcgattcga 
tcggttttg ctggagttg ttaagcttt ttttgcggt 
ttccggtca agtggtggg atgacaatg acaagtatt 
tcgtctttt tctggagtg atgacgact tgaaaagtg 
agaatttcc cggaaggtc cccagaaaa acggaaaat 
cgtgggtta cccagctgt tggacttgt cccgagttg 
ggcacaaca ccaacgttt ggatgatct ggatttcgg 
gagagatgg tccccgaga ttgaacaat tgcccattg 
agacgttga aaaaggggg tgaatttca cccttgaga 
agttcctcg gcaaggatt acaacatta ctgaaatag 
gagaagttt tgaggtcgg gttgggtca ctaatgaga 
atttccggg ggacgtatt cgttcaccc ggaacgggt 
tgtacctga aagaggtgt cgtatcaga acgacttgg 
cggagccgc tcgaaggac tgtttttcg ctggggagg 
ctacaaatc cggtgcatt ttgcgacag tgcatgggg 
ctatcgaga ttggagcgg gggcttccg gaattgccg 
caactgcga aacttttgg ataataacg ttacaaatg 
tgacaattc tggaggcgg aaaatttaa tggggggaa 
gggtctgga ctgttagtt ttggaaatg gtttggtgg 
aactagggg ctgaatatt gtaccggac aaaagggga 
attttgttt acgaagttg cccaacatt tattagaac 
caagtgaag aattatttg cacataacg tctactatt 
caaacggta ctaagctcg atgttgccc taatgaatg 
aattgggaa agtctttgg aagatttat tctaccaca 
gactgaact cgactgtgg ttcaaaaat acaaaaatg 
atgaggaga aattaagga ttgtgctga aagagtttc 
cacgacatg ctgaaacgt acattgcgc caactgaag 
gtgccttca cttggtcgg atatatctg tagataagg 
actaccaag agtgtgaag gacactcaa tggtttgga 
aaaaagtgg gctacaaaa cgatttttg attttctct 
tgaaaaaac actcaatcg aagacaaac tccacttga 
attcaaaag tgactcaaa tcaattgga accaaagcg 
aacttgtca cagtctaca cttctgata acaaatcgt 
actctgccg attttgtca tcttcaccc catccgttg 
gagtcttaa aacacgaga aaactttat ttaatccgc 
ctctcccgt cttcaaaac agcaaagta tcaaagcca 
ccggttttg ctggagtca tgaaagcat tcgtccagt 
tccgaacaa aatggtggc ttgacaacg acaccgaat 
tttccttcc tctggagcg agaacgact tgaaaaaca 
cttcgttca catcaggac ccagcaaaa acgggattc 
cttgggtgt cccaattga ccgatttcc tgaaagttc 
cgcataatc ccaaagtgt gggtttggt ggatcagcg 
gagacttaa tccccgaac ttgagaaac ttcctccag 
aaacaatga aagcgggtt ttgtctacg tcttggaca 
agtttctcg ggaaaaatt acaacgttt ctgagattg 
aggcaattg tgacgccga aatggtaca caactgaac 
atttccggg gggtttact cgttcacca aaaccggat 
tttacgaaa aagggttct cgcaccagg aaaaactgg 
gagagcctt tggtgggag tttcgggaa agccggcgg 
tgttgtttg caggagaag ccacaaatc gggttcatt 
atgcgaccg ttcatgggg ccattgaga cgggatttc 
gggaagctg gacgaatta ttcaaactc tttgacttt 
gttttttat taatcagca aaaactaca tgacatgac 
ctttattac atcacactg aaattttgt aacaaattc 
ataaacatt tcatcagtt ttgataaat ccttgagcg 
gaagatttg attatttta tatttttta ggagaacct 
atatttaga atggaattt tctaaattt acttgacat 
ataattggt aaaacacaa attaatgct gaataattg 
aaaaagttg tttatcaga caaatcttt tttttttct 
cattgatga gcaaatacc gcagattca tggaggccc 
ggagactaa atctgccta agaaataaa aagtattac 
tgtctagtt ataaataat tagatattt tttgtgtct 
ttttcgcaa aaaaatgcc tattttaat gtctccttc 
cataatatt ccttcaata atttttaac acagtttaa 
cactaaaaa aaaacatca gctaatgca ttcgcgccc 
cgaatctag tacaccaaa tttccgcaa ttttttttt 
gattcccta atgattttt tctgatttt tctatatct 
ttcagtttt agtatttat tgaacagac ggtataaaa 
aaaattttg ttcagtcta cgtcaagga tttctccac 
tttgtgatt tgtttgagt ttgctttct tgatgtaat 
gtaaaattt gctatttga ataataaat ttctggtcc 
atatagaga ggagaaact ccagactta agtaaattg 
ctatcagat caatacgtt atttattta gttccaatt 
tttgataat aacacctaa aataaaaga tctagctca 
aaattatct ttaatttca cgatcttag caaagacga 
aagcagtgt tttttagat agcagttag tttggaaat 
tattttttc taattagtt ttttgtctt gcagcgtaa 
aaatttaaa aagaaacta aagcatcaa attgaaa

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY