General information | |||
TC033596 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 3564110..3568333 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
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TC033596 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01588 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
head mostlyremaining - Penetrance (percentage of animals/eggs on slide): less than 30%
metathorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC033596 RA
MHFQFLAEN RIGGRINTV KFGDGLVEL GAEYCHGEV GNIVKELVN GYDLLEPNF NYLNGEIYY SNGSKLDHG FVREMQDLI LSENKEENY DTRGKSIGE VFMHKYNST LVEKYKSEE NKLKLLKEG LHFAERSIL ISEGSFSWF DASADSDWL ECPGNQTLV WKGVGYKTV LEILMKSYP NPDEKLPLD DKLFLNSKV TKINWGEKP IKVHTSDKV YSADYVIFT PSIGVLKAG SDLFTPSLP PKKHKAIDS IGFAGVVKL FLRFPVKWW DDNDKYFVF FWSDDDLKS ENFPEGPQK NGKSWVTQL LDLSRVGHN TNVWMIWIS GEMVPEIEQ LPIETLKKG VNFTLEKFL GKDYNITEI GEVLRSGWV TNENFRGTY SFTRNGLYL KEVSYQNDL AEPLEGLFF AGEATNPVH FATVHGAIE IGAGASGIA ATAKLLDNN VTNVTILEA ENLMGGRVW TVSFGNGLV ELGAEYCTG QKGNFVYEV AQHLLEPSE ELFAHNVYY SNGTKLDVA LMNELGKSL EDLFYHRLN STVVQKYKN DEEKLRIVL KEFPRHAET YIAPTEGAF TWSDISVDK DYQECEGHS MVWKKVGYK TIFDFLLKK HSIEDKLHL NSKVTQINW NQSELVTVY TSDNKSYSA DFVIFTPSV GVLKHEKTL FNPPLPSSK QQSIKATGF AGVMKAFVQ FRTKWWLDN DTEFSFLWS ENDLKNTSF TSGPSKNGI PWVSQLTDF LKVPHNPKV WVWWISGDL IPELEKLPP ETMKAGFVY VLDKFLGKN YNVSEIEAI VTPKWYTTE HFRGVYSFT KTGFYEKGF SHQEKLGEP LVGVSGKPA VLFAGEATN RVHYATVHG AIETGFREA GRIIQTL
- >TC033596 RA
atgcatttt caattctta gccgaaaat cgcattgga gggcgaatt aacaccgtc aagttcgga gatgggttg gttgagcta ggggctgag tactgtcat ggggaggtg ggaaacatc gtcaaggag ctggtcaat ggttatgat ttgcttgag cccaatttt aattacctc aatggggag atttactac tcgaatggg tctaaactg gaccatggg ttcgtgcgg gagatgcag gacttgatt ttgagcgaa aacaaggag gagaattac gatactcgg gggaaatcg attggggag gttttcatg cacaaatac aattcaacc cttgtcgaa aaatacaaa agcgaagaa aacaaattg aaattgctc aaagaaggg ttgcatttt gccgaacgt tcaatcctg atttctgaa ggttccttc tcatggttt gacgcttcg gccgactcc gattggttg gagtgtccc gggaaccaa acattggtc tggaaaggg gtgggttac aaaaccgtc cttgaaata ctgatgaaa agttatccc aacccggac gaaaaactg cccttagac gataaattg ttcctcaat tctaaagta acgaaaatc aactggggg gaaaaaccc atcaaagtc cacacttct gacaaggtt tactccgcc gattatgtg attttcact ccttcaatt ggggttttg aaagccggg agtgactta ttcacccct tctctcccg cctaagaaa cacaaagcc atcgattcg atcggtttt gctggagtt gttaagctt tttttgcgg tttccggtc aagtggtgg gatgacaat gacaagtat ttcgtcttt ttctggagt gatgacgac ttgaaaagt gagaatttc ccggaaggt ccccagaaa aacggaaaa tcgtgggtt acccagctg ttggacttg tcccgagtt gggcacaac accaacgtt tggatgatc tggatttcg ggagagatg gtccccgag attgaacaa ttgcccatt gagacgttg aaaaagggg gtgaatttc acccttgag aagttcctc ggcaaggat tacaacatt actgaaata ggagaagtt ttgaggtcg ggttgggtc actaatgag aatttccgg gggacgtat tcgttcacc cggaacggg ttgtacctg aaagaggtg tcgtatcag aacgacttg gcggagccg ctcgaagga ctgtttttc gctggggag gctacaaat ccggtgcat tttgcgaca gtgcatggg gctatcgag attggagcg ggggcttcc ggaattgcc gcaactgcg aaacttttg gataataac gttacaaat gtgacaatt ctggaggcg gaaaattta atgggggga agggtctgg actgttagt tttggaaat ggtttggtg gaactaggg gctgaatat tgtaccgga caaaagggg aattttgtt tacgaagtt gcccaacat ttattagaa ccaagtgaa gaattattt gcacataac gtctactat tcaaacggt actaagctc gatgttgcc ctaatgaat gaattggga aagtctttg gaagattta ttctaccac agactgaac tcgactgtg gttcaaaaa tacaaaaat gatgaggag aaattaagg attgtgctg aaagagttt ccacgacat gctgaaacg tacattgcg ccaactgaa ggtgccttc acttggtcg gatatatct gtagataag gactaccaa gagtgtgaa ggacactca atggtttgg aaaaaagtg ggctacaaa acgattttt gattttctc ttgaaaaaa cactcaatc gaagacaaa ctccacttg aattcaaaa gtgactcaa atcaattgg aaccaaagc gaacttgtc acagtctac acttctgat aacaaatcg tactctgcc gattttgtc atcttcacc ccatccgtt ggagtctta aaacacgag aaaacttta tttaatccg cctctcccg tcttcaaaa cagcaaagt atcaaagcc accggtttt gctggagtc atgaaagca ttcgtccag ttccgaaca aaatggtgg cttgacaac gacaccgaa ttttccttc ctctggagc gagaacgac ttgaaaaac acttcgttc acatcagga cccagcaaa aacgggatt ccttgggtg tcccaattg accgatttc ctgaaagtt ccgcataat cccaaagtg tgggtttgg tggatcagc ggagactta atccccgaa cttgagaaa cttcctcca gaaacaatg aaagcgggt tttgtctac gtcttggac aagtttctc gggaaaaat tacaacgtt tctgagatt gaggcaatt gtgacgccg aaatggtac acaactgaa catttccgg ggggtttac tcgttcacc aaaaccgga ttttacgaa aaagggttc tcgcaccag gaaaaactg ggagagcct ttggtggga gtttcggga aagccggcg gtgttgttt gcaggagaa gccacaaat cgggttcat tatgcgacc gttcatggg gccattgag acgggattt cgggaagct ggacgaatt attcaaact ctttga
- >TC033596 RA
gctaacttt gggtgtcgt ttgtggctt accacgaca tgcattttc aattcttag ccgaaaatc gcattggag ggcgaatta acaccgtca agttcggag atgggttgg ttgagctag gggctgagt actgtcatg gggaggtgg gaaacatcg tcaaggagc tggtcaatg gttatgatt tgcttgagc ccaatttta attacctca atggggaga tttactact cgaatgggt ctaaactgg accatgggt tcgtgcggg agatgcagg acttgattt tgagcgaaa acaaggagg agaattacg atactcggg ggaaatcga ttggggagg ttttcatgc acaaataca attcaaccc ttgtcgaaa aatacaaaa gcgaagaaa acaaattga aattgctca aagaagggt tgcattttg ccgaacgtt caatcctga tttctgaag gttccttct catggtttg acgcttcgg ccgactccg attggttgg agtgtcccg ggaaccaaa cattggtct ggaaagggg tgggttaca aaaccgtcc ttgaaatac tgatgaaaa gttatccca acccggacg aaaaactgc ccttagacg ataaattgt tcctcaatt ctaaagtaa cgaaaatca actgggggg aaaaaccca tcaaagtcc acacttctg acaaggttt actccgccg attatgtga ttttcactc cttcaattg gggttttga aagccggga gtgacttat tcacccctt ctctcccgc ctaagaaac acaaagcca tcgattcga tcggttttg ctggagttg ttaagcttt ttttgcggt ttccggtca agtggtggg atgacaatg acaagtatt tcgtctttt tctggagtg atgacgact tgaaaagtg agaatttcc cggaaggtc cccagaaaa acggaaaat cgtgggtta cccagctgt tggacttgt cccgagttg ggcacaaca ccaacgttt ggatgatct ggatttcgg gagagatgg tccccgaga ttgaacaat tgcccattg agacgttga aaaaggggg tgaatttca cccttgaga agttcctcg gcaaggatt acaacatta ctgaaatag gagaagttt tgaggtcgg gttgggtca ctaatgaga atttccggg ggacgtatt cgttcaccc ggaacgggt tgtacctga aagaggtgt cgtatcaga acgacttgg cggagccgc tcgaaggac tgtttttcg ctggggagg ctacaaatc cggtgcatt ttgcgacag tgcatgggg ctatcgaga ttggagcgg gggcttccg gaattgccg caactgcga aacttttgg ataataacg ttacaaatg tgacaattc tggaggcgg aaaatttaa tggggggaa gggtctgga ctgttagtt ttggaaatg gtttggtgg aactagggg ctgaatatt gtaccggac aaaagggga attttgttt acgaagttg cccaacatt tattagaac caagtgaag aattatttg cacataacg tctactatt caaacggta ctaagctcg atgttgccc taatgaatg aattgggaa agtctttgg aagatttat tctaccaca gactgaact cgactgtgg ttcaaaaat acaaaaatg atgaggaga aattaagga ttgtgctga aagagtttc cacgacatg ctgaaacgt acattgcgc caactgaag gtgccttca cttggtcgg atatatctg tagataagg actaccaag agtgtgaag gacactcaa tggtttgga aaaaagtgg gctacaaaa cgatttttg attttctct tgaaaaaac actcaatcg aagacaaac tccacttga attcaaaag tgactcaaa tcaattgga accaaagcg aacttgtca cagtctaca cttctgata acaaatcgt actctgccg attttgtca tcttcaccc catccgttg gagtcttaa aacacgaga aaactttat ttaatccgc ctctcccgt cttcaaaac agcaaagta tcaaagcca ccggttttg ctggagtca tgaaagcat tcgtccagt tccgaacaa aatggtggc ttgacaacg acaccgaat tttccttcc tctggagcg agaacgact tgaaaaaca cttcgttca catcaggac ccagcaaaa acgggattc cttgggtgt cccaattga ccgatttcc tgaaagttc cgcataatc ccaaagtgt gggtttggt ggatcagcg gagacttaa tccccgaac ttgagaaac ttcctccag aaacaatga aagcgggtt ttgtctacg tcttggaca agtttctcg ggaaaaatt acaacgttt ctgagattg aggcaattg tgacgccga aatggtaca caactgaac atttccggg gggtttact cgttcacca aaaccggat tttacgaaa aagggttct cgcaccagg aaaaactgg gagagcctt tggtgggag tttcgggaa agccggcgg tgttgtttg caggagaag ccacaaatc gggttcatt atgcgaccg ttcatgggg ccattgaga cgggatttc gggaagctg gacgaatta ttcaaactc tttgacttt gttttttat taatcagca aaaactaca tgacatgac ctttattac atcacactg aaattttgt aacaaattc ataaacatt tcatcagtt ttgataaat ccttgagcg gaagatttg attatttta tatttttta ggagaacct atatttaga atggaattt tctaaattt acttgacat ataattggt aaaacacaa attaatgct gaataattg aaaaagttg tttatcaga caaatcttt tttttttct cattgatga gcaaatacc gcagattca tggaggccc ggagactaa atctgccta agaaataaa aagtattac tgtctagtt ataaataat tagatattt tttgtgtct ttttcgcaa aaaaatgcc tattttaat gtctccttc cataatatt ccttcaata atttttaac acagtttaa cactaaaaa aaaacatca gctaatgca ttcgcgccc cgaatctag tacaccaaa tttccgcaa ttttttttt gattcccta atgattttt tctgatttt tctatatct ttcagtttt agtatttat tgaacagac ggtataaaa aaaattttg ttcagtcta cgtcaagga tttctccac tttgtgatt tgtttgagt ttgctttct tgatgtaat gtaaaattt gctatttga ataataaat ttctggtcc atatagaga ggagaaact ccagactta agtaaattg ctatcagat caatacgtt atttattta gttccaatt tttgataat aacacctaa aataaaaga tctagctca aaattatct ttaatttca cgatcttag caaagacga aagcagtgt tttttagat agcagttag tttggaaat tattttttc taattagtt ttttgtctt gcagcgtaa aaatttaaa aagaaacta aagcatcaa attgaaa
