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Gene information
General information
TC009633 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid47: 1376636..1383855
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009633
    iBeetle screen iB_01587
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009633 RA
      MNIYRGRSI SEIYLASRL YDAIEDCDL SSVKTLLSR 
      GADPNLILP KVRISPLHL IIGNDSVEF AVTVVTLLL 
      QNGGNPNNQ ADDGLTPIH IAAAWGRYD ILKLLLDCG 
      GDPEIRDTN NKTAFHYAL EEGFVKCYN LLKLYVADK 
      ADVLFKEKE DCKNYDFKL DKIVVDNGS TIGEYEIIN 
      ESKTDSISK NSPKLDELP QSNSNEYII NWFNSHVQT 
      SPTATAHTA GSTSGFDDT DSFKESDEE RYTHLNNHN 
      VCFRKVYRK RSPKKIKIK EISGLTGLD STSDYATCT 
      SGASPDALE RNIFDFPDE TKSEGVSSS DLSFVSVSE 
      VYKYVDEAE GIVLYERRF LKTPTVSEG SVKSFVSSR 
      DSLPETIDF EDYDPETIR RELTSLGYN PGPITVSTR 
      RVYLKKLKQ LKKVQPVVK TVEKKAEPR VYSSELEKT 
      LCNPDWLKN ISLYKTLDE AFTKQFSTP DPSKKWREG 
      TNKSSFTYL LLDPRITNN LPFRSESLK PREVWETFL 
      SSVFYVGKG KRTRPYDHL YDAVNLWNR GLRSSECRK 
      LDKILDIWK DNCGVVCLH LFQNSIPVE AYTREAAII 
      DAINIENLC NVNRGNFYG VAATWGQRD KDKFGVFLL 
      YKAMVIFLN EGERQLCPA DIN
    CDS sequence
    • >TC009633 RA
      atgaacata tacagaggc aggagcatt agcgagatt 
      tatttggct tcacgactc tacgacgca attgaagac 
      tgcgatttg agttcggtg aaaaccctc ttatcgcga 
      ggcgctgat cccaatttg attctcccc aaagttcga 
      atttccccc cttcaccta atcatcgga aacgattcc 
      gtggagttc gcagtgact gtcgtcact cttctactt 
      caaaacggg ggaaacccc aataatcaa gccgatgat 
      ggcttaaca ccgattcac atcgccgcc gcttggggg 
      cgctatgac atcctcaag ctccttctg gattgcggg 
      ggagacccc gaaatcagg gacaccaac aacaagact 
      gcttttcat tacgcccta gaagagggc ttcgtcaaa 
      tgctacaat ttgctcaag ctttatgtg gctgataag 
      gccgacgtt ttgtttaag gagaaagaa gactgcaag 
      aattacgat tttaaattg gataagata gtggtagac 
      aatggtagt acaatcggc gaatacgaa atcatcaat 
      gagtccaaa accgacagt attagtaaa aattcgccc 
      aaattggac gaactgccc cagtccaac tcaaacgag 
      tacatcatc aactggttc aacagccac gtccaaacc 
      tcccccacc gccaccgcc cacacagcc ggaagcacc 
      tccggcttc gacgacacc gattcgttc aaagaatcg 
      gacgaggag cgatacact cacttaaac aaccacaat 
      gtctgtttc cgcaaggtt taccgcaaa agatcgccg 
      aaaaaaatc aaaatcaag gaaatcagc gggctcaca 
      ggcctggac tccaccagt gactacgcc acgtgcacg 
      tccggggct tcccccgat gctctcgaa cgcaacatt 
      tttgatttt ccggatgag accaagtcg gaaggggtc 
      agcagctcg gatttgagt tttgtgagc gtttcggag 
      gtttacaag tatgtggat gaggccgaa gggattgtt 
      ttgtacgag aggaggttt ttgaagacg cccacagtc 
      agtgagggc agtgtcaag tcgtttgtt tcctcgagg 
      gattcactc ccggaaaca attgatttc gaggattat 
      gatcctgag actatcagg agggaactg acgagtttg 
      gggtacaat ccggggccg attacggtc tcgacaagg 
      agggtttat ttgaagaag ttgaagcag ttgaagaaa 
      gtacagccg gtggtcaag acggtggag aaaaaggcg 
      gaacctaga gtgtactcc tccgagttg gagaaaacc 
      ctgtgcaac cccgactgg ttgaaaaac atctcgctg 
      tacaaaacc ctcgacgag gctttcacc aagcagttc 
      tccacgcca gacccttcg aaaaagtgg cgcgagggc 
      accaacaag tcctccttc acctacctc ctcctggac 
      ccccgaatc accaacaac ctgccgttc aggtccgag 
      tcgctcaaa cctagggag gtttgggag acgttcctg 
      tcgtcggtg ttttacgtg gggaaggga aagaggaca 
      cggccctat gaccacctc tacgacgcc gtcaatctc 
      tggaatcgg ggactgagg agctccgag tgtcgcaag 
      ttggacaaa atcctggat atttggaag gataattgc 
      ggggttgtg tgtttgcac ctcttccag aacagtatc 
      ccggtggag gcgtacacg cgagaggcg gcgattatt 
      gacgcgatc aacatcgag aatttgtgt aatgtgaac 
      agggggaat ttttacggg gtggcggcg acctggggg 
      cagagggat aaggacaag ttcggggtg tttctgctc 
      tacaaggcc atggtgatt tttttgaac gaaggggag 
      aggcagctg tgtcccgcg gatattaac tag
    mRNA sequence
    • >TC009633 RA
      aaaactaac ctacaaaaa atgtttact ttttgaatc 
      gttaaagag ctttatcgc tcggatttg tcgataaat 
      tcacgcgac tatacataa tttctcaga gaattttcg 
      aatttggtg ctacaagtg tgtttctgt tttttcctc 
      taattgcaa gacaatctt ctactgggg gtgggttat 
      gtgaaaatg aacatatac agaggcagg agcattagc 
      gagatttat ttggcttca cgactctac gacgcaatt 
      gaagactgc gatttgagt tcggtgaaa accctctta 
      tcgcgaggc gctgatccc aatttgatt ctccccaaa 
      gttcgaatt tcccccctt cacctaatc atcggaaac 
      gattccgtg gagttcgca gtgactgtc gtcactctt 
      ctacttcaa aacggggga aaccccaat aatcaagcc 
      gatgatggc ttaacaccg attcacatc gccgccgct 
      tgggggcgc tatgacatc ctcaagctc cttctggat 
      tgcggggga gaccccgaa atcagggac accaacaac 
      aagactgct tttcattac gccctagaa gagggcttc 
      gtcaaatgc tacaatttg ctcaagctt tatgtggct 
      gataaggcc gacgttttg tttaaggag aaagaagac 
      tgcaagaat tacgatttt aaattggat aagatagtg 
      gtagacaat ggtagtaca atcggcgaa tacgaaatc 
      atcaatgag tccaaaacc gacagtatt agtaaaaat 
      tcgcccaaa ttggacgaa ctgccccag tccaactca 
      aacgagtac atcatcaac tggttcaac agccacgtc 
      caaacctcc cccaccgcc accgcccac acagccgga 
      agcacctcc ggcttcgac gacaccgat tcgttcaaa 
      gaatcggac gaggagcga tacactcac ttaaacaac 
      cacaatgtc tgtttccgc aaggtttac cgcaaaaga 
      tcgccgaaa aaaatcaaa atcaaggaa atcagcggg 
      ctcacaggc ctggactcc accagtgac tacgccacg 
      tgcacgtcc ggggcttcc cccgatgct ctcgaacgc 
      aacattttt gattttccg gatgagacc aagtcggaa 
      ggggtcagc agctcggat ttgagtttt gtgagcgtt 
      tcggaggtt tacaagtat gtggatgag gccgaaggg 
      attgttttg tacgagagg aggtttttg aagacgccc 
      acagtcagt gagggcagt gtcaagtcg tttgtttcc 
      tcgagggat tcactcccg gaaacaatt gatttcgag 
      gattatgat cctgagact atcaggagg gaactgacg 
      agtttgggg tacaatccg gggccgatt acggtctcg 
      acaaggagg gtttatttg aagaagttg aagcagttg 
      aagaaagta cagccggtg gtcaagacg gtggagaaa 
      aaggcggaa cctagagtg tactcctcc gagttggag 
      aaaaccctg tgcaacccc gactggttg aaaaacatc 
      tcgctgtac aaaaccctc gacgaggct ttcaccaag 
      cagttctcc acgccagac ccttcgaaa aagtggcgc 
      gagggcacc aacaagtcc tccttcacc tacctcctc 
      ctggacccc cgaatcacc aacaacctg ccgttcagg 
      tccgagtcg ctcaaacct agggaggtt tgggagacg 
      ttcctgtcg tcggtgttt tacgtgggg aagggaaag 
      aggacacgg ccctatgac cacctctac gacgccgtc 
      aatctctgg aatcgggga ctgaggagc tccgagtgt 
      cgcaagttg gacaaaatc ctggatatt tggaaggat 
      aattgcggg gttgtgtgt ttgcacctc ttccagaac 
      agtatcccg gtggaggcg tacacgcga gaggcggcg 
      attattgac gcgatcaac atcgagaat ttgtgtaat 
      gtgaacagg gggaatttt tacggggtg gcggcgacc 
      tgggggcag agggataag gacaagttc ggggtgttt 
      ctgctctac aaggccatg gtgattttt ttgaacgaa 
      ggggagagg cagctgtgt cccgcggat attaactag 
      ggttctgca gtttgtatt tacttcaca gtattaggc 
      ggacattat ttgatgaaa ttgtacact gttttattt 
      atacgatta tgtctattc tatgaataa aaaataact 
      tgctcaaat aatgctagt tttataatt agatttgtt 
      aacattcaa atatggcaa taaactttt tacattgat