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Gene information
General information
TC009632 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid47: 1371446..1375565
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009632
    iBeetle screen iB_01586
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009632 RA
      MGQDEAPDC TDRCKSTQH GDIITAAIS RRCVSDFDL 
      CSYISYICT CCESVKDSL GRTALHVAA SCGRLELVR 
      WLIHKRHAN INIRDDESG FTPLHRSIF YGKIHVAVE 
      LIKLGANTS LLDANSLTV LEHAMLDGY TPEAKCGEL 
      YAWGSNCNN SLGPQQSRS TPELLDVFH KKYPDESVR 
      KVVCEKFHS VIVTSGGLV YTCGHGQGG RLGLGTQRT 
      VVAPEMVTF PQTHEPITC VDVAVSRDH SVFLCSDGN 
      LYSCGLNTH RVLGLHPPP AQILVPTLV KHLEQVRHV 
      CAGRYHSVA WGPKCLYTW GLNAGQLGH KMRPDDNYV 
      TSPKAVRIL NFTETEIVA LDSSDGAIS IYTKKGDIY 
      VLHEYQCRK IASRQLNVV QVCIFGGKL NTNLSKELS 
      GEPNRELKV AALTNTGNL LIWQESVPQ LSRCIYSLN 
      RPIVVRQMT INNNGLLFV TKDGEAFQG TIKPKKKKP 
      VLPTIEKSA FHKFLDRED CISIKLSKF PRIHRAIFI 
      TSDVKGHDF CVIQVPPYK RFEAPLIME SQMKTNLGV 
      LLQECHEND DLHDVVLHV DHRYFPAHR FILATKSSY 
      FQKLLIDGD KTIKLDGYN SLIFEQFLT YVYVGDCDL 
      LKCGECPEK LKFLCEKHK ENSDEASEI IDGKMAAFE 
      VYNNKLKNQ NSSNNKQNR IKNPVRMLH EMAKKFGCF 
      DLCTILADY EMQKYTIRR KSHKTITKP LQFDKTSFV 
      EFSDVTIKC TDGKQLKAH KCILAAQSD YFSNLFSTR 
      WRGTETTVI TLPCSRSVA EALLEYLYT DTLSLKNRE 
      QDHLFKLII LADQLFITR LKEQCELLL FECLTLKNC 
      VQFLAFASI YNAEKLKLS CLRFIAANL APLLELRVF 
      DDLDELLMR ELSDFYFEE KREIWCRVI TPYSTAPLE 
      EEIVSVSSA FPVSTEEVV KTTQKRRSR HKTEPSLSK 
      SLKENICDE SIIQFPDSP PAVVTDNLE IPSRLKAIK 
      LAEEKLETD DYQPQYTKL VSKNSFEEF PLLNSPPTS 
      YNYSKSPHK FAHKIVKVS QKQRKRLSS ESAPESPKN 
      PWKIVSEVG SPPSMETSM SEIISSEKK QKENLVKIT 
      SKSLIYTQI EDKAIEDLC KFYNTENIN DEFISVERV 
      NMGAVAAPI WVPGVKK
    CDS sequence
    • >TC009632 RA
      atgggtcag gacgaagcc cccgattgc accgaccgt 
      tgcaaatca acccaacac ggtgacata atcacggct 
      gcaatttcc cggcgttgt gtctccgat tttgacctc 
      tgttcctac ataagctac atttgcacc tgttgcgag 
      tccgtgaaa gactcctta ggacgcaca gccctccat 
      gtcgccgcg tcgtgcgga agactcgag ctcgtaagg 
      tggttaata cacaaacga cacgcaaac attaacatt 
      agggacgac gaatcgggc ttcaccccc ttacacagg 
      agcattttt tacggcaaa atccacgtc gccgtcgag 
      ctcatcaaa ttaggggcc aacacgagc ctcctagac 
      gccaacagt ctcacagtc ctcgagcat gcaatgctc 
      gacggctac acgcccgag gcaaaatgc ggcgagttg 
      tacgcctgg ggctccaat tgcaacaac tctctgggg 
      ccccagcag tcgcgctcc acccccgaa ctcctcgac 
      gtcttccac aagaaatat ccagacgag agcgtgcga 
      aaagtcgtc tgtgagaag tttcacagt gttattgtg 
      acgtcaggg gggctggtg tacacctgt gggcatggg 
      caaggcggc aggctgggg ctaggcacc caacgcacg 
      gtcgtggcg cccgaaatg gtcaccttc ccacaaacc 
      catgaaccc atcacgtgc gtggatgtg gccgtttcg 
      agggaccat agcgtcttt ttgtgctcg gacggcaat 
      ctctattcc tgcgggttg aacacgcac cgagtgctc 
      gggctgcac cccccgccg gcgcaaatc ctcgtacca 
      acccttgtc aagcattta gagcaagtg cggcacgtg 
      tgcgctggc cgttaccac tcagtggca tgggggccc 
      aagtgtctc tacacatgg gggctcaat gccgggcaa 
      ttaggccac aaaatgcgt cccgatgac aattacgtt 
      acgtcacca aaggcggtc agaattctc aattttact 
      gagaccgaa atcgtagca ctggattcc agcgatggt 
      gcgatttcg atctataca aaaaagggc gacatctat 
      gtcttacac gagtaccaa tgtcgtaaa atcgcctca 
      aggcaatta aacgtcgtc caagtttgt attttcggc 
      gggaaatta aacacgaat ttgagcaag gaattgtca 
      ggcgagcca aatcgtgaa ttaaaagtg gccgcattg 
      accaacacc gggaatttg ttgatatgg caggaatct 
      gtaccgcaa ctaagccgt tgcatttat tcgctaaat 
      cggccaatt gttgttaga caaatgacg atcaataat 
      aacggactt ttgtttgtc acgaaagac ggtgaggcg 
      ttccaaggc accatcaag ccgaagaaa aagaaacct 
      gtgttaccg acgattgag aaaagcgcg tttcataag 
      ttcctggac cgggaggat tgtatttcg atcaaactt 
      tcgaaattt ccgcgcatt catcgcgcc attttcatc 
      acgagtgac gtcaaaggg cacgatttt tgcgtcatt 
      caagtgccc ccctataag cggtttgaa gccccactt 
      ataatggag tcacagatg aaaaccaat ttgggcgtt 
      ttattgcaa gagtgccat gaaaacgac gacttgcat 
      gacgttgta ttgcacgtg gatcaccgg tacttcccc 
      gcacatcgt tttattcta gccaccaaa agttcctac 
      tttcaaaaa ttactaata gatggcgac aaaacgatc 
      aaattggac ggctacaat tcgctcatt tttgagcaa 
      tttttaaca tatgtgtat gtcggtgat tgcgatttg 
      ctgaaatgt ggggaatgt cccgaaaaa cttaaattt 
      ttgtgtgaa aaacacaaa gaaaacagc gacgaagcg 
      agtgagata atcgacggt aaaatggcc gcgtttgaa 
      gtatataac aacaaattg aaaaatcaa aatagtagc 
      aataataaa cagaatagg attaaaaac ccagtcagg 
      atgctacac gaaatggcc aaaaaattc ggttgtttc 
      gacctttgt acgatttta gccgattac gaaatgcaa 
      aaatacaca attcggcgc aaaagtcac aaaactatt 
      acgaaacct ttacaattt gataaaaca tcattcgtc 
      gaatttagc gatgtcaca atcaagtgc accgatgga 
      aaacaattg aaagctcac aaatgcatt ttggcggcg 
      cagagcgac tatttcagc aatttgttt tcgacgagg 
      tggcgtggg acggaaact accgttatc acgctcccg 
      tgttcgcgg agcgttgca gaggcattg ttggagtat 
      ttgtacacc gacactctc tccttgaaa aatagggaa 
      caggaccat ttgtttaag ttgattatt ttggcggac 
      caattgttc atcactcgc ttgaaggaa caatgcgag 
      ttattattg ttcgagtgt ttaactttg aaaaactgc 
      gtccagttt ttggcattt gcgagcatt tacaacgct 
      gagaagttg aaattgagt tgtttgagg tttattgct 
      gcgaatttg gctccactg ttggagttg cgcgttttt 
      gacgatttg gacgagttg ttgatgagg gaattgagt 
      gatttttat ttcgaggag aagagggag atttggtgt 
      cgggttata acgccctac tccacagcc cccttggag 
      gaggagatt gtctcagtg agttctgca tttcccgtt 
      tcgacggaa gaggtggtg aaaacgacg caaaaaagg 
      cgttcgaga cacaaaact gagccgagt ttgagcaaa 
      tctttgaag gaaaatatc tgtgatgaa agtattatt 
      caattccct gattctcct cctgctgtg gtcactgat 
      aatttagaa attcccagt cgattaaaa gccataaaa 
      ttggccgaa gaaaaactg gaaactgac gattatcag 
      ccacaatac acgaaacta gtttcgaaa aattccttc 
      gaagagttt cccctttta aatagtcct ccaacttcc 
      tacaattac tccaaatcg cctcataaa ttcgcgcac 
      aaaatcgtg aaagtctcg caaaagcag aggaaaaga 
      ctttcatcg gaaagcgct ccagaaagt ccgaaaaat 
      ccctggaaa atcgttagc gaggtcggg agtccgccc 
      tctatggaa accagcatg agcgaaatt atttcgtcc 
      gagaaaaaa cagaaggaa aatctagtc aagattacg 
      agcaaatca ttgatttat acacaaata gaagataag 
      gcaattgag gatctctgt aagttttac aacacggaa 
      aatatcaac gacgagttt atttcagtc gaacgggtc 
      aacatgggg gctgtggcg gcccccatt tgggtccca 
      ggtgttaaa aagtga
    mRNA sequence
    • >TC009632 RA
      cgtcatcga gttgtcaaa ctttttgtc caattttat 
      tatgcattt tttgcattt aattcgcat caattagag 
      ggacaggga tgggtcagg acgaagccc ccgattgca 
      ccgaccgtt gcaaatcaa cccaacacg gtgacataa 
      tcacggctg caatttccc ggcgttgtg tctccgatt 
      ttgacctct gttcctaca taagctaca tttgcacct 
      gttgcgagt ccgtgaaag actccttag gacgcacag 
      ccctccatg tcgccgcgt cgtgcggaa gactcgagc 
      tcgtaaggt ggttaatac acaaacgac acgcaaaca 
      ttaacatta gggacgacg aatcgggct tcaccccct 
      tacacagga gcatttttt acggcaaaa tccacgtcg 
      ccgtcgagc tcatcaaat taggggcca acacgagcc 
      tcctagacg ccaacagtc tcacagtcc tcgagcatg 
      caatgctcg acggctaca cgcccgagg caaaatgcg 
      gcgagttgt acgcctggg gctccaatt gcaacaact 
      ctctggggc cccagcagt cgcgctcca cccccgaac 
      tcctcgacg tcttccaca agaaatatc cagacgaga 
      gcgtgcgaa aagtcgtct gtgagaagt ttcacagtg 
      ttattgtga cgtcagggg ggctggtgt acacctgtg 
      ggcatgggc aaggcggca ggctggggc taggcaccc 
      aacgcacgg tcgtggcgc ccgaaatgg tcaccttcc 
      cacaaaccc atgaaccca tcacgtgcg tggatgtgg 
      ccgtttcga gggaccata gcgtctttt tgtgctcgg 
      acggcaatc tctattcct gcgggttga acacgcacc 
      gagtgctcg ggctgcacc ccccgccgg cgcaaatcc 
      tcgtaccaa cccttgtca agcatttag agcaagtgc 
      ggcacgtgt gcgctggcc gttaccact cagtggcat 
      gggggccca agtgtctct acacatggg ggctcaatg 
      ccgggcaat taggccaca aaatgcgtc ccgatgaca 
      attacgtta cgtcaccaa aggcggtca gaattctca 
      attttactg agaccgaaa tcgtagcac tggattcca 
      gcgatggtg cgatttcga tctatacaa aaaagggcg 
      acatctatg tcttacacg agtaccaat gtcgtaaaa 
      tcgcctcaa ggcaattaa acgtcgtcc aagtttgta 
      ttttcggcg ggaaattaa acacgaatt tgagcaagg 
      aattgtcag gcgagccaa atcgtgaat taaaagtgg 
      ccgcattga ccaacaccg ggaatttgt tgatatggc 
      aggaatctg taccgcaac taagccgtt gcatttatt 
      cgctaaatc ggccaattg ttgttagac aaatgacga 
      tcaataata acggacttt tgtttgtca cgaaagacg 
      gtgaggcgt tccaaggca ccatcaagc cgaagaaaa 
      agaaacctg tgttaccga cgattgaga aaagcgcgt 
      ttcataagt tcctggacc gggaggatt gtatttcga 
      tcaaacttt cgaaatttc cgcgcattc atcgcgcca 
      ttttcatca cgagtgacg tcaaagggc acgattttt 
      gcgtcattc aagtgcccc cctataagc ggtttgaag 
      ccccactta taatggagt cacagatga aaaccaatt 
      tgggcgttt tattgcaag agtgccatg aaaacgacg 
      acttgcatg acgttgtat tgcacgtgg atcaccggt 
      acttccccg cacatcgtt ttattctag ccaccaaaa 
      gttcctact ttcaaaaat tactaatag atggcgaca 
      aaacgatca aattggacg gctacaatt cgctcattt 
      ttgagcaat ttttaacat atgtgtatg tcggtgatt 
      gcgatttgc tgaaatgtg gggaatgtc ccgaaaaac 
      ttaaatttt tgtgtgaaa aacacaaag aaaacagcg 
      acgaagcga gtgagataa tcgacggta aaatggccg 
      cgtttgaag tatataaca acaaattga aaaatcaaa 
      atagtagca ataataaac agaatagga ttaaaaacc 
      cagtcagga tgctacacg aaatggcca aaaaattcg 
      gttgtttcg acctttgta cgattttag ccgattacg 
      aaatgcaaa aatacacaa ttcggcgca aaagtcaca 
      aaactatta cgaaacctt tacaatttg ataaaacat 
      cattcgtcg aatttagcg atgtcacaa tcaagtgca 
      ccgatggaa aacaattga aagctcaca aatgcattt 
      tggcggcgc agagcgact atttcagca atttgtttt 
      cgacgaggt ggcgtggga cggaaacta ccgttatca 
      cgctcccgt gttcgcgga gcgttgcag aggcattgt 
      tggagtatt tgtacaccg acactctct ccttgaaaa 
      atagggaac aggaccatt tgtttaagt tgattattt 
      tggcggacc aattgttca tcactcgct tgaaggaac 
      aatgcgagt tattattgt tcgagtgtt taactttga 
      aaaactgcg tccagtttt tggcatttg cgagcattt 
      acaacgctg agaagttga aattgagtt gtttgaggt 
      ttattgctg cgaatttgg ctccactgt tggagttgc 
      gcgtttttg acgatttgg acgagttgt tgatgaggg 
      aattgagtg atttttatt tcgaggaga agagggaga 
      tttggtgtc gggttataa cgccctact ccacagccc 
      ccttggagg aggagattg tctcagtga gttctgcat 
      ttcccgttt cgacggaag aggtggtga aaacgacgc 
      aaaaaaggc gttcgagac acaaaactg agccgagtt 
      tgagcaaat ctttgaagg aaaatatct gtgatgaaa 
      gtattattc aattccctg attctcctc ctgctgtgg 
      tcactgata atttagaaa ttcccagtc gattaaaag 
      ccataaaat tggccgaag aaaaactgg aaactgacg 
      attatcagc cacaataca cgaaactag tttcgaaaa 
      attccttcg aagagtttc cccttttaa atagtcctc 
      caacttcct acaattact ccaaatcgc ctcataaat 
      tcgcgcaca aaatcgtga aagtctcgc aaaagcaga 
      ggaaaagac tttcatcgg aaagcgctc cagaaagtc 
      cgaaaaatc cctggaaaa tcgttagcg aggtcggga 
      gtccgccct ctatggaaa ccagcatga gcgaaatta 
      tttcgtccg agaaaaaac agaaggaaa atctagtca 
      agattacga gcaaatcat tgatttata cacaaatag 
      aagataagg caattgagg atctctgta agttttaca 
      acacggaaa atatcaacg acgagttta tttcagtcg 
      aacgggtca acatggggg ctgtggcgg cccccattt 
      gggtcccag gtgttaaaa agtgatttt agttttgtt 
      gacggtgta aattttatt ttaataaag ttgttttat 
      ttat