General information | |||
TC009632 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 3453346..3457465 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC009632 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01586 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
- >TC009632 RA
MGQDEAPDC TDRCKSTQH GDIITAAIS RRCVSDFDL CSYISYICT CCESVKDSL GRTALHVAA SCGRLELVR WLIHKRHAN INIRDDESG FTPLHRSIF YGKIHVAVE LIKLGANTS LLDANSLTV LEHAMLDGY TPEAKCGEL YAWGSNCNN SLGPQQSRS TPELLDVFH KKYPDESVR KVVCEKFHS VIVTSGGLV YTCGHGQGG RLGLGTQRT VVAPEMVTF PQTHEPITC VDVAVSRDH SVFLCSDGN LYSCGLNTH RVLGLHPPP AQILVPTLV KHLEQVRHV CAGRYHSVA WGPKCLYTW GLNAGQLGH KMRPDDNYV TSPKAVRIL NFTETEIVA LDSSDGAIS IYTKKGDIY VLHEYQCRK IASRQLNVV QVCIFGGKL NTNLSKELS GEPNRELKV AALTNTGNL LIWQESVPQ LSRCIYSLN RPIVVRQMT INNNGLLFV TKDGEAFQG TIKPKKKKP VLPTIEKSA FHKFLDRED CISIKLSKF PRIHRAIFI TSDVKGHDF CVIQVPPYK RFEAPLIME SQMKTNLGV LLQECHEND DLHDVVLHV DHRYFPAHR FILATKSSY FQKLLIDGD KTIKLDGYN SLIFEQFLT YVYVGDCDL LKCGECPEK LKFLCEKHK ENSDEASEI IDGKMAAFE VYNNKLKNQ NSSNNKQNR IKNPVRMLH EMAKKFGCF DLCTILADY EMQKYTIRR KSHKTITKP LQFDKTSFV EFSDVTIKC TDGKQLKAH KCILAAQSD YFSNLFSTR WRGTETTVI TLPCSRSVA EALLEYLYT DTLSLKNRE QDHLFKLII LADQLFITR LKEQCELLL FECLTLKNC VQFLAFASI YNAEKLKLS CLRFIAANL APLLELRVF DDLDELLMR ELSDFYFEE KREIWCRVI TPYSTAPLE EEIVSVSSA FPVSTEEVV KTTQKRRSR HKTEPSLSK SLKENICDE SIIQFPDSP PAVVTDNLE IPSRLKAIK LAEEKLETD DYQPQYTKL VSKNSFEEF PLLNSPPTS YNYSKSPHK FAHKIVKVS QKQRKRLSS ESAPESPKN PWKIVSEVG SPPSMETSM SEIISSEKK QKENLVKIT SKSLIYTQI EDKAIEDLC KFYNTENIN DEFISVERV NMGAVAAPI WVPGVKK
- >TC009632 RA
atgggtcag gacgaagcc cccgattgc accgaccgt tgcaaatca acccaacac ggtgacata atcacggct gcaatttcc cggcgttgt gtctccgat tttgacctc tgttcctac ataagctac atttgcacc tgttgcgag tccgtgaaa gactcctta ggacgcaca gccctccat gtcgccgcg tcgtgcgga agactcgag ctcgtaagg tggttaata cacaaacga cacgcaaac attaacatt agggacgac gaatcgggc ttcaccccc ttacacagg agcattttt tacggcaaa atccacgtc gccgtcgag ctcatcaaa ttaggggcc aacacgagc ctcctagac gccaacagt ctcacagtc ctcgagcat gcaatgctc gacggctac acgcccgag gcaaaatgc ggcgagttg tacgcctgg ggctccaat tgcaacaac tctctgggg ccccagcag tcgcgctcc acccccgaa ctcctcgac gtcttccac aagaaatat ccagacgag agcgtgcga aaagtcgtc tgtgagaag tttcacagt gttattgtg acgtcaggg gggctggtg tacacctgt gggcatggg caaggcggc aggctgggg ctaggcacc caacgcacg gtcgtggcg cccgaaatg gtcaccttc ccacaaacc catgaaccc atcacgtgc gtggatgtg gccgtttcg agggaccat agcgtcttt ttgtgctcg gacggcaat ctctattcc tgcgggttg aacacgcac cgagtgctc gggctgcac cccccgccg gcgcaaatc ctcgtacca acccttgtc aagcattta gagcaagtg cggcacgtg tgcgctggc cgttaccac tcagtggca tgggggccc aagtgtctc tacacatgg gggctcaat gccgggcaa ttaggccac aaaatgcgt cccgatgac aattacgtt acgtcacca aaggcggtc agaattctc aattttact gagaccgaa atcgtagca ctggattcc agcgatggt gcgatttcg atctataca aaaaagggc gacatctat gtcttacac gagtaccaa tgtcgtaaa atcgcctca aggcaatta aacgtcgtc caagtttgt attttcggc gggaaatta aacacgaat ttgagcaag gaattgtca ggcgagcca aatcgtgaa ttaaaagtg gccgcattg accaacacc gggaatttg ttgatatgg caggaatct gtaccgcaa ctaagccgt tgcatttat tcgctaaat cggccaatt gttgttaga caaatgacg atcaataat aacggactt ttgtttgtc acgaaagac ggtgaggcg ttccaaggc accatcaag ccgaagaaa aagaaacct gtgttaccg acgattgag aaaagcgcg tttcataag ttcctggac cgggaggat tgtatttcg atcaaactt tcgaaattt ccgcgcatt catcgcgcc attttcatc acgagtgac gtcaaaggg cacgatttt tgcgtcatt caagtgccc ccctataag cggtttgaa gccccactt ataatggag tcacagatg aaaaccaat ttgggcgtt ttattgcaa gagtgccat gaaaacgac gacttgcat gacgttgta ttgcacgtg gatcaccgg tacttcccc gcacatcgt tttattcta gccaccaaa agttcctac tttcaaaaa ttactaata gatggcgac aaaacgatc aaattggac ggctacaat tcgctcatt tttgagcaa tttttaaca tatgtgtat gtcggtgat tgcgatttg ctgaaatgt ggggaatgt cccgaaaaa cttaaattt ttgtgtgaa aaacacaaa gaaaacagc gacgaagcg agtgagata atcgacggt aaaatggcc gcgtttgaa gtatataac aacaaattg aaaaatcaa aatagtagc aataataaa cagaatagg attaaaaac ccagtcagg atgctacac gaaatggcc aaaaaattc ggttgtttc gacctttgt acgatttta gccgattac gaaatgcaa aaatacaca attcggcgc aaaagtcac aaaactatt acgaaacct ttacaattt gataaaaca tcattcgtc gaatttagc gatgtcaca atcaagtgc accgatgga aaacaattg aaagctcac aaatgcatt ttggcggcg cagagcgac tatttcagc aatttgttt tcgacgagg tggcgtggg acggaaact accgttatc acgctcccg tgttcgcgg agcgttgca gaggcattg ttggagtat ttgtacacc gacactctc tccttgaaa aatagggaa caggaccat ttgtttaag ttgattatt ttggcggac caattgttc atcactcgc ttgaaggaa caatgcgag ttattattg ttcgagtgt ttaactttg aaaaactgc gtccagttt ttggcattt gcgagcatt tacaacgct gagaagttg aaattgagt tgtttgagg tttattgct gcgaatttg gctccactg ttggagttg cgcgttttt gacgatttg gacgagttg ttgatgagg gaattgagt gatttttat ttcgaggag aagagggag atttggtgt cgggttata acgccctac tccacagcc cccttggag gaggagatt gtctcagtg agttctgca tttcccgtt tcgacggaa gaggtggtg aaaacgacg caaaaaagg cgttcgaga cacaaaact gagccgagt ttgagcaaa tctttgaag gaaaatatc tgtgatgaa agtattatt caattccct gattctcct cctgctgtg gtcactgat aatttagaa attcccagt cgattaaaa gccataaaa ttggccgaa gaaaaactg gaaactgac gattatcag ccacaatac acgaaacta gtttcgaaa aattccttc gaagagttt cccctttta aatagtcct ccaacttcc tacaattac tccaaatcg cctcataaa ttcgcgcac aaaatcgtg aaagtctcg caaaagcag aggaaaaga ctttcatcg gaaagcgct ccagaaagt ccgaaaaat ccctggaaa atcgttagc gaggtcggg agtccgccc tctatggaa accagcatg agcgaaatt atttcgtcc gagaaaaaa cagaaggaa aatctagtc aagattacg agcaaatca ttgatttat acacaaata gaagataag gcaattgag gatctctgt aagttttac aacacggaa aatatcaac gacgagttt atttcagtc gaacgggtc aacatgggg gctgtggcg gcccccatt tgggtccca ggtgttaaa aagtga
- >TC009632 RA
cgtcatcga gttgtcaaa ctttttgtc caattttat tatgcattt tttgcattt aattcgcat caattagag ggacaggga tgggtcagg acgaagccc ccgattgca ccgaccgtt gcaaatcaa cccaacacg gtgacataa tcacggctg caatttccc ggcgttgtg tctccgatt ttgacctct gttcctaca taagctaca tttgcacct gttgcgagt ccgtgaaag actccttag gacgcacag ccctccatg tcgccgcgt cgtgcggaa gactcgagc tcgtaaggt ggttaatac acaaacgac acgcaaaca ttaacatta gggacgacg aatcgggct tcaccccct tacacagga gcatttttt acggcaaaa tccacgtcg ccgtcgagc tcatcaaat taggggcca acacgagcc tcctagacg ccaacagtc tcacagtcc tcgagcatg caatgctcg acggctaca cgcccgagg caaaatgcg gcgagttgt acgcctggg gctccaatt gcaacaact ctctggggc cccagcagt cgcgctcca cccccgaac tcctcgacg tcttccaca agaaatatc cagacgaga gcgtgcgaa aagtcgtct gtgagaagt ttcacagtg ttattgtga cgtcagggg ggctggtgt acacctgtg ggcatgggc aaggcggca ggctggggc taggcaccc aacgcacgg tcgtggcgc ccgaaatgg tcaccttcc cacaaaccc atgaaccca tcacgtgcg tggatgtgg ccgtttcga gggaccata gcgtctttt tgtgctcgg acggcaatc tctattcct gcgggttga acacgcacc gagtgctcg ggctgcacc ccccgccgg cgcaaatcc tcgtaccaa cccttgtca agcatttag agcaagtgc ggcacgtgt gcgctggcc gttaccact cagtggcat gggggccca agtgtctct acacatggg ggctcaatg ccgggcaat taggccaca aaatgcgtc ccgatgaca attacgtta cgtcaccaa aggcggtca gaattctca attttactg agaccgaaa tcgtagcac tggattcca gcgatggtg cgatttcga tctatacaa aaaagggcg acatctatg tcttacacg agtaccaat gtcgtaaaa tcgcctcaa ggcaattaa acgtcgtcc aagtttgta ttttcggcg ggaaattaa acacgaatt tgagcaagg aattgtcag gcgagccaa atcgtgaat taaaagtgg ccgcattga ccaacaccg ggaatttgt tgatatggc aggaatctg taccgcaac taagccgtt gcatttatt cgctaaatc ggccaattg ttgttagac aaatgacga tcaataata acggacttt tgtttgtca cgaaagacg gtgaggcgt tccaaggca ccatcaagc cgaagaaaa agaaacctg tgttaccga cgattgaga aaagcgcgt ttcataagt tcctggacc gggaggatt gtatttcga tcaaacttt cgaaatttc cgcgcattc atcgcgcca ttttcatca cgagtgacg tcaaagggc acgattttt gcgtcattc aagtgcccc cctataagc ggtttgaag ccccactta taatggagt cacagatga aaaccaatt tgggcgttt tattgcaag agtgccatg aaaacgacg acttgcatg acgttgtat tgcacgtgg atcaccggt acttccccg cacatcgtt ttattctag ccaccaaaa gttcctact ttcaaaaat tactaatag atggcgaca aaacgatca aattggacg gctacaatt cgctcattt ttgagcaat ttttaacat atgtgtatg tcggtgatt gcgatttgc tgaaatgtg gggaatgtc ccgaaaaac ttaaatttt tgtgtgaaa aacacaaag aaaacagcg acgaagcga gtgagataa tcgacggta aaatggccg cgtttgaag tatataaca acaaattga aaaatcaaa atagtagca ataataaac agaatagga ttaaaaacc cagtcagga tgctacacg aaatggcca aaaaattcg gttgtttcg acctttgta cgattttag ccgattacg aaatgcaaa aatacacaa ttcggcgca aaagtcaca aaactatta cgaaacctt tacaatttg ataaaacat cattcgtcg aatttagcg atgtcacaa tcaagtgca ccgatggaa aacaattga aagctcaca aatgcattt tggcggcgc agagcgact atttcagca atttgtttt cgacgaggt ggcgtggga cggaaacta ccgttatca cgctcccgt gttcgcgga gcgttgcag aggcattgt tggagtatt tgtacaccg acactctct ccttgaaaa atagggaac aggaccatt tgtttaagt tgattattt tggcggacc aattgttca tcactcgct tgaaggaac aatgcgagt tattattgt tcgagtgtt taactttga aaaactgcg tccagtttt tggcatttg cgagcattt acaacgctg agaagttga aattgagtt gtttgaggt ttattgctg cgaatttgg ctccactgt tggagttgc gcgtttttg acgatttgg acgagttgt tgatgaggg aattgagtg atttttatt tcgaggaga agagggaga tttggtgtc gggttataa cgccctact ccacagccc ccttggagg aggagattg tctcagtga gttctgcat ttcccgttt cgacggaag aggtggtga aaacgacgc aaaaaaggc gttcgagac acaaaactg agccgagtt tgagcaaat ctttgaagg aaaatatct gtgatgaaa gtattattc aattccctg attctcctc ctgctgtgg tcactgata atttagaaa ttcccagtc gattaaaag ccataaaat tggccgaag aaaaactgg aaactgacg attatcagc cacaataca cgaaactag tttcgaaaa attccttcg aagagtttc cccttttaa atagtcctc caacttcct acaattact ccaaatcgc ctcataaat tcgcgcaca aaatcgtga aagtctcgc aaaagcaga ggaaaagac tttcatcgg aaagcgctc cagaaagtc cgaaaaatc cctggaaaa tcgttagcg aggtcggga gtccgccct ctatggaaa ccagcatga gcgaaatta tttcgtccg agaaaaaac agaaggaaa atctagtca agattacga gcaaatcat tgatttata cacaaatag aagataagg caattgagg atctctgta agttttaca acacggaaa atatcaacg acgagttta tttcagtcg aacgggtca acatggggg ctgtggcgg cccccattt gggtcccag gtgttaaaa agtgatttt agttttgtt gacggtgta aattttatt ttaataaag ttgttttat ttat
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
