| General information | |||
| TC034769 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 3184753..3192268 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC034769 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01583 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
- >TC034769 RA
MSLSCARIT VTCLIIVVC VVFSQCQDL FIVNRYKPD EYYCITNSS NALSWVDED TSQDIHFKA SRFIPSPTE KNLWSVSCN SKNDQSPLG WNCETALSG TVILDRRWE DLQEYKTDY NEESNDNMS NVKLFINIP ITLEENFTL PLSIRIKEN AHIYLCDGK DPPNSNCYW ISIGTGALK KTILQKCSS SQGASDKKE NCFKQRAVF ANENLNSAY WTHFRLFKT AKTLLFTYE NNGKSKEIF SCCNEDNKI FSTTNLIIS STNFVGRWK FHQITYYKT TKAQNDTPF GSPLKPIGD SVCISVFIN MCESCQITF KLTNQENRI LIKKTYGYE KDWREIRFN TDEIKDIVT LLVSTHITT KEENQNQFW ALDNMKQCH RDGFRMISL DKSASCQLL SESGSISVT RNKINDVSA NCRINSVGK SCMPCSWFL NDKCGQLKI CEKYNNAIS CSCSAGYTT SNSLKCDSE CADGSYGHQ CKKKCDVRC KSNNCSHLN GRCTTWCAN KNETCNIWF VEPPRLKNA SYVEAVVEV SNNELIGDE CADCTYILQ YKEVEGNKR QWHTALENN TFLKTQSIK IKELKPGTD YYVRYFIVK GKNTNAFDE NIPKTNFST LCKEIQRED LSIDPSNTS ITLSTPTKK NPMTCNLKK SSIEKLNSN FRIEARINE SDTPIIILG DLYPYTSYN VQISPKLKL SLMTGEGVP DIVQELKVV NKSDTSVHL LWKRPSRLN GPVENYVVY YQTGLRLGC EKDNPKGNL SEKFERNTT KPTIILENL VPFTHYMFT VYAVNTKFE SKRVTYWDS TTESHSIKE EECPKLKQI KPDNRSVQT QFSSIDCDK IEGSISIEI SAICEEEWC MIKNVTNIF NYTTENTAS TISSNIYPF SKYHLTVKC CRNDCIYIE KHKEFKTTV EPPYKVKDF LVFSKNEST ISVRWRQPY PPTGILDHY NISYYITKE SEGKSEIVT HSPCKLWIS FQCLTLSNL EADTKYFIQ IRARNTGSK NYGQLIEEE ATTKTEASK PPHNLIVDW TLQNDLQLM WFHPNESNG YITHFNISV LSGNQNENK IVNIFKVVS HNLTYTHTI NQRKFQPST YYNITIQAF NGVNGHPVS TQDLSPPAI PFFENEPKH SITNTTITI EISKVKNNI KDFKLFLLI ENDTTDTII YPKELKQFE EKFHNQQSN FRILYSSTN IQSPLTFMI GKTNQTFQN KSTSHLDFP LKTENSYNI SILLINTYK DKSSYKFYS FHIPKLLKP LVLPAVSTD LSLLSLLFL LLIIPLGIV VFIKRDTIK QKLNSLIQR NNTANMEVS EDQTPLQRL DNNQNIYTK KIKVSELQK YIKESLKND ELERQHKFF PRGQTKPWD CGSSPSNKN KNRYTNLLP YDHTRVVLK KIQGEEHSD YINANYIDG YNMPKAYIA TQGPKKNTI GDFWRMVWQ ENVRHIVML ANLYENEKK KVEKYWPEM GENLRYGTI TLQYFSNRV YADYEHRVF KVTNGNSTR EVQQYHFQS WPDHGVPLY AQTLIPFLE NLQKIPLST KSPIVVHCS AGVGRTGTI ILCDSCLRM AVSEKCVDV LGTLQKIRN QRPNMVDNV EQYKLAHLV ILDYLDGIR TGIPCSEIG EVEELLKSD ELKKQMRYL ETTAWQDEI FMPTVLQNE TSFVVKKKN RFQNIIPGN HRYIFLPRY PIDDESSIY INAVTVDGF KHPDRFIVT QQPLPNTLG DFWRLVHDK DISVIISLN EIDLNYTTC NFWPSEDNK ELNPVDFLT LKHAKTLIC DTYDVITVH MHDQNNTDQ KVIEILALK NWPAMNSCP DKIDSFLTF WEESYATSR QSGQVIVTC FDGALASGL YVSMSFILE KIKLEQVCD VCQAVRIVR HNRKQFVEK EEQFIFLYK AAMTYIGGF EIYANFN
- >TC034769 RA
atgagtctt agttgcgcc agaataact gtgacatgt ttaattatt gttgtgtgt gttgtgttt tcacaatgc caagatctt tttattgtt aatagatat aaaccagat gaatattac tgcattacc aatagttca aatgcttta tcttgggtg gatgaagat acatctcaa gatatacat tttaaagca tctagattt ataccttca cccacagaa aaaaacctt tggagtgtc agttgtaac tccaaaaat gatcagtct cctttagga tggaattgt gaaacagca ctttctggt acagtgatt ttagatcgc cgatgggaa gatttacaa gagtacaaa actgattat aatgaagag tcaaacgat aatatgtcg aacgtgaaa ttgtttatc aacattcct attacatta gaagaaaac tttacacta ccactgtcc attcgtatc aaagaaaac gctcatata tatctctgt gatggcaaa gatccaccc aattcaaac tgctattgg atttcaata ggaacagga gctttaaag aaaacaatt ttgcaaaaa tgttccagc tctcaagga gctagtgat aaaaaagag aactgtttt aaacaacgg gctgttttt gccaatgag aatcttaat agtgcttac tggactcac ttcagactc ttcaaaacg gcaaaaaca ctgcttttt acttatgaa aacaacgga aaatccaaa gaaatattt tcatgttgt aatgaagat aataagata ttcagtaca acaaacttg atcattagc agcacaaat ttcgtcggt cgttggaaa ttccatcaa ataacttac tacaagacc acaaaagct caaaatgat acaccattt ggaagtccc ctaaaaccc ataggagac tccgtgtgc atttccgtg tttataaat atgtgtgaa tcttgtcaa attactttt aagttaact aaccaagaa aatagaata ttaattaaa aaaacttac ggatacgag aaagactgg cgagaaatc agatttaat acagatgaa ataaaagac attgtaacc ctattagta tctacacat ataacaaca aaagaagaa aatcaaaat cagttttgg gcacttgat aatatgaaa caatgtcac agagacggt tttcgaatg atttcttta gataaatca gcaagttgt caacttctg tccgaaagc ggtagcatt tcagtgaca agaaacaaa attaatgat gtttcagca aactgcaga ataaattca gtcggcaaa tcatgtatg ccgtgttct tggtttcta aatgacaaa tgcggacaa ttaaaaata tgtgagaaa tataataac gccatctct tgttcatgt tccgctgga tatacaact tcaaactca ctcaaatgt gattctgag tgtgctgat gggtcttat ggtcatcaa tgtaaaaag aaatgtgat gtaagatgt aaaagtaat aattgttcc caccttaac ggaagatgt acgacatgg tgtgccaat aaaaatgag acatgcaat atatggttt gtagaacct cctagattg aaaaatgct tcttatgta gaagctgtc gtcgaagtc tccaacaat gaattaatt ggtgacgaa tgtgcagat tgcacgtac attcttcaa tataaggaa gtagaagga aataagcga caatggcat actgcgtta gaaaacaac acgttttta aaaacacag tcaattaaa ataaaagaa ctgaaacca ggcactgac tattatgtc aggtatttc atcgttaaa ggcaaaaac acaaatgca tttgatgaa aatatacca aaaacaaac ttttccact ttatgcaaa gaaatccaa agagaagac ctgtcgatt gatccttcc aatacaagt ataacctta tccactcct actaagaaa aaccctatg acttgtaat ttgaaaaaa tcctcgatc gagaaactc aattccaat tttagaatt gaggctaga attaacgag tccgataca ccaataata attttgggt gatttatat ccttataca agttacaat gtacaaatt tctccaaaa ctaaaatta tccttgatg actggagaa ggagttcca gatattgtt caagaacta aaagtagtt aataaatca gacacaagt gtacactta ttgtggaaa cgaccttct cgactcaat ggtcctgtg gaaaactat gtcgtttac tatcagact ggactacgc ttgggttgt gaaaaagac aacccgaaa ggaaatcta tccgaaaaa tttgaacgt aacacaact aaaccaaca ataatttta gaaaactta gttccattt acacattat atgtttaca gtatatgcc gttaataca aaattcgaa agcaagcgg gtaacctat tgggattcc actacagag tctcattca attaaagag gaagaatgt ccaaaattg aaacaaata aaaccagat aatcgttcc gttcaaaca caattttct tccattgat tgtgataaa attgaagga tcaatttct atcgaaatt tcagcaatt tgtgaagaa gagtggtgc atgattaaa aacgtgact aatattttt aattacacc accgagaac actgcgtcg accatatcg tcaaacata tatcccttt tcaaaatat catttaaca gttaaatgt tgccgaaac gactgcatt tacattgag aaacataaa gaatttaaa acaacggta gagccgccg tataaagtt aaagacttt ttagttttt tcgaagaat gaaagtact atttcagtc aggtggaga caaccgtat ccaccaact ggtatatta gaccactat aacatttca tattacatt actaaagaa agcgaagga aaatctgaa attgtaaca cattctccc tgtaaacta tggattagt ttccagtgc ttgacctta agtaattta gaagctgat acaaaatat tttatacaa attcgggcc agaaatacc ggatcgaag aattatggg caacttata gaagaagaa gcaacaacc aaaacggag gcttcgaaa cctccacat aatctcatc gttgattgg actttacaa aacgacctt caattaatg tggtttcac ccaaatgaa tccaatgga tatataact catttcaat atttccgtt ctttctggt aatcaaaat gagaacaaa atcgttaat atattcaaa gtagtaagc cacaacctt acttatact cacaccatc aaccaaaga aaatttcaa ccttcaacg tattacaac atcacgatt caagcgttc aacggtgta aatggccat cctgtgagc actcaagat ctaagtccc cctgctatc ccatttttt gaaaatgag cctaaacat tcaataacg aacactacc attactata gaaatatcc aaagttaag aacaacatc aaggatttt aaattattt ttattaata gaaaatgac accaccgac actatcata tatcccaaa gagttaaaa caatttgaa gaaaagttt cataatcag caatcaaat tttagaatt ttgtacagt agtaccaac attcaaagt cctttaacg tttatgatt ggtaaaacg aatcagact tttcaaaac aaaagcacg agtcatctg gattttcct ctaaaaact gaaaacagc tacaacata tccatttta ttaatcaac acatacaag gataaaagt agctacaag ttttattct tttcatatt cccaaatta ttaaaacct ttagtactg ccggccgta agcacagat ttgagtctg ctatcactt ttatttcta ttactaata attcctcta ggtatagta gtttttatc aaaagagat acaattaag caaaagtta aactccctc atacaaaga aataataca gctaatatg gaagtttca gaagatcaa acaccacta caaagatta gacaataat caaaacatt tatacaaaa aaaatcaaa gtgtccgaa ctgcaaaaa tacattaaa gagtctttg aaaaatgac gaacttgag cgccagcac aagtttttt ccacgagga caaacaaaa ccgtgggat tgcggttct tcaccaagc aacaaaaat aaaaaccga tacacgaat ttattacca tatgaccat acaagagta gtacttaaa aaaattcaa ggtgaagaa cactcagac tacattaac gctaactac attgacggt tacaatatg ccaaaagcg tacattgcc acacaagga ccaaaaaaa aacacaatt ggtgatttt tggcgaatg gtttggcaa gaaaacgtg agacatatc gtcatgttg gccaacctc tacgaaaac gaaaagaaa aaagtggag aaatattgg cccgagatg ggcgagaat ttacgttat ggtacaatc acacttcaa tatttttca aatcgagtc tatgcggac tacgagcat cgagtattt aaagtcacc aatggcaat agtaccagg gaggtacag cagtaccac ttccagtca tggcccgac catggggta ccactttac gcccaaaca cttatccca tttttggaa aacctccag aaaatccca cttagtacc aaatcaccg attgtagtc cactgcagt gctggagtt ggcagaact ggtactata attttgtgt gattcttgc ttaagaatg gccgttagt gagaaatgt gtagatgtg ttgggaaca cttcaaaaa attcgcaac caaagaccc aacatggtc gataatgtc gaacaatac aaactggcg catttggtc attttggac tatttggat ggtatccgc actggaata ccatgtagc gagattgga gaagtggaa gaattgtta aaaagtgac gaattgaag aaacagatg cgttatctg gagactaca gcttggcaa gacgagata ttcatgcca acagtgtta caaaatgaa acttcattt gtggttaaa aaaaaaaat cgcttccag aacattatt cccggcaat cacaggtac atcttcttg cctcgttat ccaatcgat gacgaatct tcaatttac ataaacgcg gttactgtc gatggtttc aaacaccct gatcgtttt attgtgaca caacaaccg ttaccaaac accctcgga gatttttgg cgcctggtc cacgataag gacatttca gtgatcatt tccttaaac gaaatcgac ttaaactac acgacttgc aacttttgg ccgtctgaa gacaacaaa gagctgaat ccggttgat ttccttact ctcaaacac gccaaaacc cttatatgt gatacctat gacgttata accgttcac atgcatgat caaaataat actgaccaa aaagtgatc gaaatactt gcattaaaa aattggccg gcgatgaat agctgtccg gacaaaatt gacagtttt ttgactttt tgggaagag tcgtatgcg acttccagg cagtctggt caagttatt gtcacttgc tttgatgga gcacttgcg tccggtctg tacgtctcc atgtcgttc attttggaa aaaattaaa ttagagcaa gtttgtgac gtttgccaa gctgtgaga attgtgaga cataatagg aaacaattc gtcgagaaa gaagaacaa tttattttt ttgtataaa gctgcgatg acttatatt ggtggattt gagatttat gctaatttt aattag
- >TC034769 RA
tgtctgcaa atgtcgaaa tatttttgc catgagtct tagttgcgc cagaataac tgtgacatg tttaattat tgttgtgtg tgttgtgtt ttcacaatg ccaagatct ttttattgt taatagata taaaccaga tgaatatta ctgcattac caatagttc aaatgcttt atcttgggt ggatgaaga tacatctca agatataca ttttaaagc atctagatt tataccttc acccacaga aaaaaacct ttggagtgt cagttgtaa ctccaaaaa tgatcagtc tcctttagg atggaattg tgaaacagc actttctgg tacagtgat tttagatcg ccgatggga agatttaca agagtacaa aactgatta taatgaaga gtcaaacga taatatgtc gaacgtgaa attgtttat caacattcc tattacatt agaagaaaa ctttacact accactgtc cattcgtat caaagaaaa cgctcatat atatctctg tgatggcaa agatccacc caattcaaa ctgctattg gatttcaat aggaacagg agctttaaa gaaaacaat tttgcaaaa atgttccag ctctcaagg agctagtga taaaaaaga gaactgttt taaacaacg ggctgtttt tgccaatga gaatcttaa tagtgctta ctggactca cttcagact cttcaaaac ggcaaaaac actgctttt tacttatga aaacaacgg aaaatccaa agaaatatt ttcatgttg taatgaaga taataagat attcagtac aacaaactt gatcattag cagcacaaa tttcgtcgg tcgttggaa attccatca aataactta ctacaagac cacaaaagc tcaaaatga tacaccatt tggaagtcc cctaaaacc cataggaga ctccgtgtg catttccgt gtttataaa tatgtgtga atcttgtca aattacttt taagttaac taaccaaga aaatagaat attaattaa aaaaactta cggatacga gaaagactg gcgagaaat cagatttaa tacagatga aataaaaga cattgtaac cctattagt atctacaca tataacaac aaaagaaga aaatcaaaa tcagttttg ggcacttga taatatgaa acaatgtca cagagacgg ttttcgaat gatttcttt agataaatc agcaagttg tcaacttct gtccgaaag cggtagcat ttcagtgac aagaaacaa aattaatga tgtttcagc aaactgcag aataaattc agtcggcaa atcatgtat gccgtgttc ttggtttct aaatgacaa atgcggaca attaaaaat atgtgagaa atataataa cgccatctc ttgttcatg ttccgctgg atatacaac ttcaaactc actcaaatg tgattctga gtgtgctga tgggtctta tggtcatca atgtaaaaa gaaatgtga tgtaagatg taaaagtaa taattgttc ccaccttaa cggaagatg tacgacatg gtgtgccaa taaaaatga gacatgcaa tatatggtt tgtagaacc tcctagatt gaaaaatgc ttcttatgt agaagctgt cgtcgaagt ctccaacaa tgaattaat tggtgacga atgtgcaga ttgcacgta cattcttca atataagga agtagaagg aaataagcg acaatggca tactgcgtt agaaaacaa cacgttttt aaaaacaca gtcaattaa aataaaaga actgaaacc aggcactga ctattatgt caggtattt catcgttaa aggcaaaaa cacaaatgc atttgatga aaatatacc aaaaacaaa cttttccac tttatgcaa agaaatcca aagagaaga cctgtcgat tgatccttc caatacaag tataacctt atccactcc tactaagaa aaaccctat gacttgtaa tttgaaaaa atcctcgat cgagaaact caattccaa ttttagaat tgaggctag aattaacga gtccgatac accaataat aattttggg tgatttata tccttatac aagttacaa tgtacaaat ttctccaaa actaaaatt atccttgat gactggaga aggagttcc agatattgt tcaagaact aaaagtagt taataaatc agacacaag tgtacactt attgtggaa acgaccttc tcgactcaa tggtcctgt ggaaaacta tgtcgttta ctatcagac tggactacg cttgggttg tgaaaaaga caacccgaa aggaaatct atccgaaaa atttgaacg taacacaac taaaccaac aataatttt agaaaactt agttccatt tacacatta tatgtttac agtatatgc cgttaatac aaaattcga aagcaagcg ggtaaccta ttgggattc cactacaga gtctcattc aattaaaga ggaagaatg tccaaaatt gaaacaaat aaaaccaga taatcgttc cgttcaaac acaattttc ttccattga ttgtgataa aattgaagg atcaatttc tatcgaaat ttcagcaat ttgtgaaga agagtggtg catgattaa aaacgtgac taatatttt taattacac caccgagaa cactgcgtc gaccatatc gtcaaacat atatccctt ttcaaaata tcatttaac agttaaatg ttgccgaaa cgactgcat ttacattga gaaacataa agaatttaa aacaacggt agagccgcc gtataaagt taaagactt tttagtttt ttcgaagaa tgaaagtac tatttcagt caggtggag acaaccgta tccaccaac tggtatatt agaccacta taacatttc atattacat tactaaaga aagcgaagg aaaatctga aattgtaac acattctcc ctgtaaact atggattag tttccagtg cttgacctt aagtaattt agaagctga tacaaaata ttttataca aattcgggc cagaaatac cggatcgaa gaattatgg gcaacttat agaagaaga agcaacaac caaaacgga ggcttcgaa acctccaca taatctcat cgttgattg gactttaca aaacgacct tcaattaat gtggtttca cccaaatga atccaatgg atatataac tcatttcaa tatttccgt tctttctgg taatcaaaa tgagaacaa aatcgttaa tatattcaa agtagtaag ccacaacct tacttatac tcacaccat caaccaaag aaaatttca accttcaac gtattacaa catcacgat tcaagcgtt caacggtgt aaatggcca tcctgtgag cactcaaga tctaagtcc ccctgctat cccattttt tgaaaatga gcctaaaca ttcaataac gaacactac cattactat agaaatatc caaagttaa gaacaacat caaggattt taaattatt tttattaat agaaaatga caccaccga cactatcat atatcccaa agagttaaa acaatttga agaaaagtt tcataatca gcaatcaaa ttttagaat tttgtacag tagtaccaa cattcaaag tcctttaac gtttatgat tggtaaaac gaatcagac ttttcaaaa caaaagcac gagtcatct ggattttcc tctaaaaac tgaaaacag ctacaacat atccatttt attaatcaa cacatacaa ggataaaag tagctacaa gttttattc ttttcatat tcccaaatt attaaaacc tttagtact gccggccgt aagcacaga tttgagtct gctatcact tttatttct attactaat aattcctct aggtatagt agtttttat caaaagaga tacaattaa gcaaaagtt aaactccct catacaaag aaataatac agctaatat ggaagtttc agaagatca aacaccact acaaagatt agacaataa tcaaaacat ttatacaaa aaaaatcaa agtgtccga actgcaaaa atacattaa agagtcttt gaaaaatga cgaacttga gcgccagca caagttttt tccacgagg acaaacaaa accgtggga ttgcggttc ttcaccaag caacaaaaa taaaaaccg atacacgaa tttattacc atatgacca tacaagagt agtacttaa aaaaattca aggtgaaga acactcaga ctacattaa cgctaacta cattgacgg ttacaatat gccaaaagc gtacattgc cacacaagg accaaaaaa aaacacaat tggtgattt ttggcgaat ggtttggca agaaaacgt gagacatat cgtcatgtt ggccaacct ctacgaaaa cgaaaagaa aaaagtgga gaaatattg gcccgagat gggcgagaa tttacgtta tggtacaat cacacttca atatttttc aaatcgagt ctatgcgga ctacgagca tcgagtatt taaagtcac caatggcaa tagtaccag ggaggtaca gcagtacca cttccagtc atggcccga ccatggggt accacttta cgcccaaac acttatccc atttttgga aaacctcca gaaaatccc acttagtac caaatcacc gattgtagt ccactgcag tgctggagt tggcagaac tggtactat aattttgtg tgattcttg cttaagaat ggccgttag tgagaaatg tgtagatgt gttgggaac acttcaaaa aattcgcaa ccaaagacc caacatggt cgataatgt cgaacaata caaactggc gcatttggt cattttgga ctatttgga tggtatccg cactggaat accatgtag cgagattgg agaagtgga agaattgtt aaaaagtga cgaattgaa gaaacagat gcgttatct ggagactac agcttggca agacgagat attcatgcc aacagtgtt acaaaatga aacttcatt tgtggttaa aaaaaaaaa tcgcttcca gaacattat tcccggcaa tcacaggta catcttctt gcctcgtta tccaatcga tgacgaatc ttcaattta cataaacgc ggttactgt cgatggttt caaacaccc tgatcgttt tattgtgac acaacaacc gttaccaaa caccctcgg agatttttg gcgcctggt ccacgataa ggacatttc agtgatcat ttccttaaa cgaaatcga cttaaacta cacgacttg caacttttg gccgtctga agacaacaa agagctgaa tccggttga tttccttac tctcaaaca cgccaaaac ccttatatg tgataccta tgacgttat aaccgttca catgcatga tcaaaataa tactgacca aaaagtgat cgaaatact tgcattaaa aaattggcc ggcgatgaa tagctgtcc ggacaaaat tgacagttt tttgacttt ttgggaaga gtcgtatgc gacttccag gcagtctgg tcaagttat tgtcacttg ctttgatgg agcacttgc gtccggtct gtacgtctc catgtcgtt cattttgga aaaaattaa attagagca agtttgtga cgtttgcca agctgtgag aattgtgag acataatag gaaacaatt cgtcgagaa agaagaaca atttatttt tttgtataa agctgcgat gacttatat tggtggatt tgagattta tgctaattt taattagac atactttaa ttatcgaac tgtacctaa attaattga gtgaataaa ttttgtttg aaaat
