Status
Please wait ...
Gene information
General information
TC034769 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3184753..3192268
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034769
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01583

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034769 RA
      MSLSCARIT VTCLIIVVC VVFSQCQDL FIVNRYKPD 
      EYYCITNSS NALSWVDED TSQDIHFKA SRFIPSPTE 
      KNLWSVSCN SKNDQSPLG WNCETALSG TVILDRRWE 
      DLQEYKTDY NEESNDNMS NVKLFINIP ITLEENFTL 
      PLSIRIKEN AHIYLCDGK DPPNSNCYW ISIGTGALK 
      KTILQKCSS SQGASDKKE NCFKQRAVF ANENLNSAY 
      WTHFRLFKT AKTLLFTYE NNGKSKEIF SCCNEDNKI 
      FSTTNLIIS STNFVGRWK FHQITYYKT TKAQNDTPF 
      GSPLKPIGD SVCISVFIN MCESCQITF KLTNQENRI 
      LIKKTYGYE KDWREIRFN TDEIKDIVT LLVSTHITT 
      KEENQNQFW ALDNMKQCH RDGFRMISL DKSASCQLL 
      SESGSISVT RNKINDVSA NCRINSVGK SCMPCSWFL 
      NDKCGQLKI CEKYNNAIS CSCSAGYTT SNSLKCDSE 
      CADGSYGHQ CKKKCDVRC KSNNCSHLN GRCTTWCAN 
      KNETCNIWF VEPPRLKNA SYVEAVVEV SNNELIGDE 
      CADCTYILQ YKEVEGNKR QWHTALENN TFLKTQSIK 
      IKELKPGTD YYVRYFIVK GKNTNAFDE NIPKTNFST 
      LCKEIQRED LSIDPSNTS ITLSTPTKK NPMTCNLKK 
      SSIEKLNSN FRIEARINE SDTPIIILG DLYPYTSYN 
      VQISPKLKL SLMTGEGVP DIVQELKVV NKSDTSVHL 
      LWKRPSRLN GPVENYVVY YQTGLRLGC EKDNPKGNL 
      SEKFERNTT KPTIILENL VPFTHYMFT VYAVNTKFE 
      SKRVTYWDS TTESHSIKE EECPKLKQI KPDNRSVQT 
      QFSSIDCDK IEGSISIEI SAICEEEWC MIKNVTNIF 
      NYTTENTAS TISSNIYPF SKYHLTVKC CRNDCIYIE 
      KHKEFKTTV EPPYKVKDF LVFSKNEST ISVRWRQPY 
      PPTGILDHY NISYYITKE SEGKSEIVT HSPCKLWIS 
      FQCLTLSNL EADTKYFIQ IRARNTGSK NYGQLIEEE 
      ATTKTEASK PPHNLIVDW TLQNDLQLM WFHPNESNG 
      YITHFNISV LSGNQNENK IVNIFKVVS HNLTYTHTI 
      NQRKFQPST YYNITIQAF NGVNGHPVS TQDLSPPAI 
      PFFENEPKH SITNTTITI EISKVKNNI KDFKLFLLI 
      ENDTTDTII YPKELKQFE EKFHNQQSN FRILYSSTN 
      IQSPLTFMI GKTNQTFQN KSTSHLDFP LKTENSYNI 
      SILLINTYK DKSSYKFYS FHIPKLLKP LVLPAVSTD 
      LSLLSLLFL LLIIPLGIV VFIKRDTIK QKLNSLIQR 
      NNTANMEVS EDQTPLQRL DNNQNIYTK KIKVSELQK 
      YIKESLKND ELERQHKFF PRGQTKPWD CGSSPSNKN 
      KNRYTNLLP YDHTRVVLK KIQGEEHSD YINANYIDG 
      YNMPKAYIA TQGPKKNTI GDFWRMVWQ ENVRHIVML 
      ANLYENEKK KVEKYWPEM GENLRYGTI TLQYFSNRV 
      YADYEHRVF KVTNGNSTR EVQQYHFQS WPDHGVPLY 
      AQTLIPFLE NLQKIPLST KSPIVVHCS AGVGRTGTI 
      ILCDSCLRM AVSEKCVDV LGTLQKIRN QRPNMVDNV 
      EQYKLAHLV ILDYLDGIR TGIPCSEIG EVEELLKSD 
      ELKKQMRYL ETTAWQDEI FMPTVLQNE TSFVVKKKN 
      RFQNIIPGN HRYIFLPRY PIDDESSIY INAVTVDGF 
      KHPDRFIVT QQPLPNTLG DFWRLVHDK DISVIISLN 
      EIDLNYTTC NFWPSEDNK ELNPVDFLT LKHAKTLIC 
      DTYDVITVH MHDQNNTDQ KVIEILALK NWPAMNSCP 
      DKIDSFLTF WEESYATSR QSGQVIVTC FDGALASGL 
      YVSMSFILE KIKLEQVCD VCQAVRIVR HNRKQFVEK 
      EEQFIFLYK AAMTYIGGF EIYANFN
    CDS sequence
    • >TC034769 RA
      atgagtctt agttgcgcc agaataact gtgacatgt 
      ttaattatt gttgtgtgt gttgtgttt tcacaatgc 
      caagatctt tttattgtt aatagatat aaaccagat 
      gaatattac tgcattacc aatagttca aatgcttta 
      tcttgggtg gatgaagat acatctcaa gatatacat 
      tttaaagca tctagattt ataccttca cccacagaa 
      aaaaacctt tggagtgtc agttgtaac tccaaaaat 
      gatcagtct cctttagga tggaattgt gaaacagca 
      ctttctggt acagtgatt ttagatcgc cgatgggaa 
      gatttacaa gagtacaaa actgattat aatgaagag 
      tcaaacgat aatatgtcg aacgtgaaa ttgtttatc 
      aacattcct attacatta gaagaaaac tttacacta 
      ccactgtcc attcgtatc aaagaaaac gctcatata 
      tatctctgt gatggcaaa gatccaccc aattcaaac 
      tgctattgg atttcaata ggaacagga gctttaaag 
      aaaacaatt ttgcaaaaa tgttccagc tctcaagga 
      gctagtgat aaaaaagag aactgtttt aaacaacgg 
      gctgttttt gccaatgag aatcttaat agtgcttac 
      tggactcac ttcagactc ttcaaaacg gcaaaaaca 
      ctgcttttt acttatgaa aacaacgga aaatccaaa 
      gaaatattt tcatgttgt aatgaagat aataagata 
      ttcagtaca acaaacttg atcattagc agcacaaat 
      ttcgtcggt cgttggaaa ttccatcaa ataacttac 
      tacaagacc acaaaagct caaaatgat acaccattt 
      ggaagtccc ctaaaaccc ataggagac tccgtgtgc 
      atttccgtg tttataaat atgtgtgaa tcttgtcaa 
      attactttt aagttaact aaccaagaa aatagaata 
      ttaattaaa aaaacttac ggatacgag aaagactgg 
      cgagaaatc agatttaat acagatgaa ataaaagac 
      attgtaacc ctattagta tctacacat ataacaaca 
      aaagaagaa aatcaaaat cagttttgg gcacttgat 
      aatatgaaa caatgtcac agagacggt tttcgaatg 
      atttcttta gataaatca gcaagttgt caacttctg 
      tccgaaagc ggtagcatt tcagtgaca agaaacaaa 
      attaatgat gtttcagca aactgcaga ataaattca 
      gtcggcaaa tcatgtatg ccgtgttct tggtttcta 
      aatgacaaa tgcggacaa ttaaaaata tgtgagaaa 
      tataataac gccatctct tgttcatgt tccgctgga 
      tatacaact tcaaactca ctcaaatgt gattctgag 
      tgtgctgat gggtcttat ggtcatcaa tgtaaaaag 
      aaatgtgat gtaagatgt aaaagtaat aattgttcc 
      caccttaac ggaagatgt acgacatgg tgtgccaat 
      aaaaatgag acatgcaat atatggttt gtagaacct 
      cctagattg aaaaatgct tcttatgta gaagctgtc 
      gtcgaagtc tccaacaat gaattaatt ggtgacgaa 
      tgtgcagat tgcacgtac attcttcaa tataaggaa 
      gtagaagga aataagcga caatggcat actgcgtta 
      gaaaacaac acgttttta aaaacacag tcaattaaa 
      ataaaagaa ctgaaacca ggcactgac tattatgtc 
      aggtatttc atcgttaaa ggcaaaaac acaaatgca 
      tttgatgaa aatatacca aaaacaaac ttttccact 
      ttatgcaaa gaaatccaa agagaagac ctgtcgatt 
      gatccttcc aatacaagt ataacctta tccactcct 
      actaagaaa aaccctatg acttgtaat ttgaaaaaa 
      tcctcgatc gagaaactc aattccaat tttagaatt 
      gaggctaga attaacgag tccgataca ccaataata 
      attttgggt gatttatat ccttataca agttacaat 
      gtacaaatt tctccaaaa ctaaaatta tccttgatg 
      actggagaa ggagttcca gatattgtt caagaacta 
      aaagtagtt aataaatca gacacaagt gtacactta 
      ttgtggaaa cgaccttct cgactcaat ggtcctgtg 
      gaaaactat gtcgtttac tatcagact ggactacgc 
      ttgggttgt gaaaaagac aacccgaaa ggaaatcta 
      tccgaaaaa tttgaacgt aacacaact aaaccaaca 
      ataatttta gaaaactta gttccattt acacattat 
      atgtttaca gtatatgcc gttaataca aaattcgaa 
      agcaagcgg gtaacctat tgggattcc actacagag 
      tctcattca attaaagag gaagaatgt ccaaaattg 
      aaacaaata aaaccagat aatcgttcc gttcaaaca 
      caattttct tccattgat tgtgataaa attgaagga 
      tcaatttct atcgaaatt tcagcaatt tgtgaagaa 
      gagtggtgc atgattaaa aacgtgact aatattttt 
      aattacacc accgagaac actgcgtcg accatatcg 
      tcaaacata tatcccttt tcaaaatat catttaaca 
      gttaaatgt tgccgaaac gactgcatt tacattgag 
      aaacataaa gaatttaaa acaacggta gagccgccg 
      tataaagtt aaagacttt ttagttttt tcgaagaat 
      gaaagtact atttcagtc aggtggaga caaccgtat 
      ccaccaact ggtatatta gaccactat aacatttca 
      tattacatt actaaagaa agcgaagga aaatctgaa 
      attgtaaca cattctccc tgtaaacta tggattagt 
      ttccagtgc ttgacctta agtaattta gaagctgat 
      acaaaatat tttatacaa attcgggcc agaaatacc 
      ggatcgaag aattatggg caacttata gaagaagaa 
      gcaacaacc aaaacggag gcttcgaaa cctccacat 
      aatctcatc gttgattgg actttacaa aacgacctt 
      caattaatg tggtttcac ccaaatgaa tccaatgga 
      tatataact catttcaat atttccgtt ctttctggt 
      aatcaaaat gagaacaaa atcgttaat atattcaaa 
      gtagtaagc cacaacctt acttatact cacaccatc 
      aaccaaaga aaatttcaa ccttcaacg tattacaac 
      atcacgatt caagcgttc aacggtgta aatggccat 
      cctgtgagc actcaagat ctaagtccc cctgctatc 
      ccatttttt gaaaatgag cctaaacat tcaataacg 
      aacactacc attactata gaaatatcc aaagttaag 
      aacaacatc aaggatttt aaattattt ttattaata 
      gaaaatgac accaccgac actatcata tatcccaaa 
      gagttaaaa caatttgaa gaaaagttt cataatcag 
      caatcaaat tttagaatt ttgtacagt agtaccaac 
      attcaaagt cctttaacg tttatgatt ggtaaaacg 
      aatcagact tttcaaaac aaaagcacg agtcatctg 
      gattttcct ctaaaaact gaaaacagc tacaacata 
      tccatttta ttaatcaac acatacaag gataaaagt 
      agctacaag ttttattct tttcatatt cccaaatta 
      ttaaaacct ttagtactg ccggccgta agcacagat 
      ttgagtctg ctatcactt ttatttcta ttactaata 
      attcctcta ggtatagta gtttttatc aaaagagat 
      acaattaag caaaagtta aactccctc atacaaaga 
      aataataca gctaatatg gaagtttca gaagatcaa 
      acaccacta caaagatta gacaataat caaaacatt 
      tatacaaaa aaaatcaaa gtgtccgaa ctgcaaaaa 
      tacattaaa gagtctttg aaaaatgac gaacttgag 
      cgccagcac aagtttttt ccacgagga caaacaaaa 
      ccgtgggat tgcggttct tcaccaagc aacaaaaat 
      aaaaaccga tacacgaat ttattacca tatgaccat 
      acaagagta gtacttaaa aaaattcaa ggtgaagaa 
      cactcagac tacattaac gctaactac attgacggt 
      tacaatatg ccaaaagcg tacattgcc acacaagga 
      ccaaaaaaa aacacaatt ggtgatttt tggcgaatg 
      gtttggcaa gaaaacgtg agacatatc gtcatgttg 
      gccaacctc tacgaaaac gaaaagaaa aaagtggag 
      aaatattgg cccgagatg ggcgagaat ttacgttat 
      ggtacaatc acacttcaa tatttttca aatcgagtc 
      tatgcggac tacgagcat cgagtattt aaagtcacc 
      aatggcaat agtaccagg gaggtacag cagtaccac 
      ttccagtca tggcccgac catggggta ccactttac 
      gcccaaaca cttatccca tttttggaa aacctccag 
      aaaatccca cttagtacc aaatcaccg attgtagtc 
      cactgcagt gctggagtt ggcagaact ggtactata 
      attttgtgt gattcttgc ttaagaatg gccgttagt 
      gagaaatgt gtagatgtg ttgggaaca cttcaaaaa 
      attcgcaac caaagaccc aacatggtc gataatgtc 
      gaacaatac aaactggcg catttggtc attttggac 
      tatttggat ggtatccgc actggaata ccatgtagc 
      gagattgga gaagtggaa gaattgtta aaaagtgac 
      gaattgaag aaacagatg cgttatctg gagactaca 
      gcttggcaa gacgagata ttcatgcca acagtgtta 
      caaaatgaa acttcattt gtggttaaa aaaaaaaat 
      cgcttccag aacattatt cccggcaat cacaggtac 
      atcttcttg cctcgttat ccaatcgat gacgaatct 
      tcaatttac ataaacgcg gttactgtc gatggtttc 
      aaacaccct gatcgtttt attgtgaca caacaaccg 
      ttaccaaac accctcgga gatttttgg cgcctggtc 
      cacgataag gacatttca gtgatcatt tccttaaac 
      gaaatcgac ttaaactac acgacttgc aacttttgg 
      ccgtctgaa gacaacaaa gagctgaat ccggttgat 
      ttccttact ctcaaacac gccaaaacc cttatatgt 
      gatacctat gacgttata accgttcac atgcatgat 
      caaaataat actgaccaa aaagtgatc gaaatactt 
      gcattaaaa aattggccg gcgatgaat agctgtccg 
      gacaaaatt gacagtttt ttgactttt tgggaagag 
      tcgtatgcg acttccagg cagtctggt caagttatt 
      gtcacttgc tttgatgga gcacttgcg tccggtctg 
      tacgtctcc atgtcgttc attttggaa aaaattaaa 
      ttagagcaa gtttgtgac gtttgccaa gctgtgaga 
      attgtgaga cataatagg aaacaattc gtcgagaaa 
      gaagaacaa tttattttt ttgtataaa gctgcgatg 
      acttatatt ggtggattt gagatttat gctaatttt 
      aattag
    mRNA sequence
    • >TC034769 RA
      tgtctgcaa atgtcgaaa tatttttgc catgagtct 
      tagttgcgc cagaataac tgtgacatg tttaattat 
      tgttgtgtg tgttgtgtt ttcacaatg ccaagatct 
      ttttattgt taatagata taaaccaga tgaatatta 
      ctgcattac caatagttc aaatgcttt atcttgggt 
      ggatgaaga tacatctca agatataca ttttaaagc 
      atctagatt tataccttc acccacaga aaaaaacct 
      ttggagtgt cagttgtaa ctccaaaaa tgatcagtc 
      tcctttagg atggaattg tgaaacagc actttctgg 
      tacagtgat tttagatcg ccgatggga agatttaca 
      agagtacaa aactgatta taatgaaga gtcaaacga 
      taatatgtc gaacgtgaa attgtttat caacattcc 
      tattacatt agaagaaaa ctttacact accactgtc 
      cattcgtat caaagaaaa cgctcatat atatctctg 
      tgatggcaa agatccacc caattcaaa ctgctattg 
      gatttcaat aggaacagg agctttaaa gaaaacaat 
      tttgcaaaa atgttccag ctctcaagg agctagtga 
      taaaaaaga gaactgttt taaacaacg ggctgtttt 
      tgccaatga gaatcttaa tagtgctta ctggactca 
      cttcagact cttcaaaac ggcaaaaac actgctttt 
      tacttatga aaacaacgg aaaatccaa agaaatatt 
      ttcatgttg taatgaaga taataagat attcagtac 
      aacaaactt gatcattag cagcacaaa tttcgtcgg 
      tcgttggaa attccatca aataactta ctacaagac 
      cacaaaagc tcaaaatga tacaccatt tggaagtcc 
      cctaaaacc cataggaga ctccgtgtg catttccgt 
      gtttataaa tatgtgtga atcttgtca aattacttt 
      taagttaac taaccaaga aaatagaat attaattaa 
      aaaaactta cggatacga gaaagactg gcgagaaat 
      cagatttaa tacagatga aataaaaga cattgtaac 
      cctattagt atctacaca tataacaac aaaagaaga 
      aaatcaaaa tcagttttg ggcacttga taatatgaa 
      acaatgtca cagagacgg ttttcgaat gatttcttt 
      agataaatc agcaagttg tcaacttct gtccgaaag 
      cggtagcat ttcagtgac aagaaacaa aattaatga 
      tgtttcagc aaactgcag aataaattc agtcggcaa 
      atcatgtat gccgtgttc ttggtttct aaatgacaa 
      atgcggaca attaaaaat atgtgagaa atataataa 
      cgccatctc ttgttcatg ttccgctgg atatacaac 
      ttcaaactc actcaaatg tgattctga gtgtgctga 
      tgggtctta tggtcatca atgtaaaaa gaaatgtga 
      tgtaagatg taaaagtaa taattgttc ccaccttaa 
      cggaagatg tacgacatg gtgtgccaa taaaaatga 
      gacatgcaa tatatggtt tgtagaacc tcctagatt 
      gaaaaatgc ttcttatgt agaagctgt cgtcgaagt 
      ctccaacaa tgaattaat tggtgacga atgtgcaga 
      ttgcacgta cattcttca atataagga agtagaagg 
      aaataagcg acaatggca tactgcgtt agaaaacaa 
      cacgttttt aaaaacaca gtcaattaa aataaaaga 
      actgaaacc aggcactga ctattatgt caggtattt 
      catcgttaa aggcaaaaa cacaaatgc atttgatga 
      aaatatacc aaaaacaaa cttttccac tttatgcaa 
      agaaatcca aagagaaga cctgtcgat tgatccttc 
      caatacaag tataacctt atccactcc tactaagaa 
      aaaccctat gacttgtaa tttgaaaaa atcctcgat 
      cgagaaact caattccaa ttttagaat tgaggctag 
      aattaacga gtccgatac accaataat aattttggg 
      tgatttata tccttatac aagttacaa tgtacaaat 
      ttctccaaa actaaaatt atccttgat gactggaga 
      aggagttcc agatattgt tcaagaact aaaagtagt 
      taataaatc agacacaag tgtacactt attgtggaa 
      acgaccttc tcgactcaa tggtcctgt ggaaaacta 
      tgtcgttta ctatcagac tggactacg cttgggttg 
      tgaaaaaga caacccgaa aggaaatct atccgaaaa 
      atttgaacg taacacaac taaaccaac aataatttt 
      agaaaactt agttccatt tacacatta tatgtttac 
      agtatatgc cgttaatac aaaattcga aagcaagcg 
      ggtaaccta ttgggattc cactacaga gtctcattc 
      aattaaaga ggaagaatg tccaaaatt gaaacaaat 
      aaaaccaga taatcgttc cgttcaaac acaattttc 
      ttccattga ttgtgataa aattgaagg atcaatttc 
      tatcgaaat ttcagcaat ttgtgaaga agagtggtg 
      catgattaa aaacgtgac taatatttt taattacac 
      caccgagaa cactgcgtc gaccatatc gtcaaacat 
      atatccctt ttcaaaata tcatttaac agttaaatg 
      ttgccgaaa cgactgcat ttacattga gaaacataa 
      agaatttaa aacaacggt agagccgcc gtataaagt 
      taaagactt tttagtttt ttcgaagaa tgaaagtac 
      tatttcagt caggtggag acaaccgta tccaccaac 
      tggtatatt agaccacta taacatttc atattacat 
      tactaaaga aagcgaagg aaaatctga aattgtaac 
      acattctcc ctgtaaact atggattag tttccagtg 
      cttgacctt aagtaattt agaagctga tacaaaata 
      ttttataca aattcgggc cagaaatac cggatcgaa 
      gaattatgg gcaacttat agaagaaga agcaacaac 
      caaaacgga ggcttcgaa acctccaca taatctcat 
      cgttgattg gactttaca aaacgacct tcaattaat 
      gtggtttca cccaaatga atccaatgg atatataac 
      tcatttcaa tatttccgt tctttctgg taatcaaaa 
      tgagaacaa aatcgttaa tatattcaa agtagtaag 
      ccacaacct tacttatac tcacaccat caaccaaag 
      aaaatttca accttcaac gtattacaa catcacgat 
      tcaagcgtt caacggtgt aaatggcca tcctgtgag 
      cactcaaga tctaagtcc ccctgctat cccattttt 
      tgaaaatga gcctaaaca ttcaataac gaacactac 
      cattactat agaaatatc caaagttaa gaacaacat 
      caaggattt taaattatt tttattaat agaaaatga 
      caccaccga cactatcat atatcccaa agagttaaa 
      acaatttga agaaaagtt tcataatca gcaatcaaa 
      ttttagaat tttgtacag tagtaccaa cattcaaag 
      tcctttaac gtttatgat tggtaaaac gaatcagac 
      ttttcaaaa caaaagcac gagtcatct ggattttcc 
      tctaaaaac tgaaaacag ctacaacat atccatttt 
      attaatcaa cacatacaa ggataaaag tagctacaa 
      gttttattc ttttcatat tcccaaatt attaaaacc 
      tttagtact gccggccgt aagcacaga tttgagtct 
      gctatcact tttatttct attactaat aattcctct 
      aggtatagt agtttttat caaaagaga tacaattaa 
      gcaaaagtt aaactccct catacaaag aaataatac 
      agctaatat ggaagtttc agaagatca aacaccact 
      acaaagatt agacaataa tcaaaacat ttatacaaa 
      aaaaatcaa agtgtccga actgcaaaa atacattaa 
      agagtcttt gaaaaatga cgaacttga gcgccagca 
      caagttttt tccacgagg acaaacaaa accgtggga 
      ttgcggttc ttcaccaag caacaaaaa taaaaaccg 
      atacacgaa tttattacc atatgacca tacaagagt 
      agtacttaa aaaaattca aggtgaaga acactcaga 
      ctacattaa cgctaacta cattgacgg ttacaatat 
      gccaaaagc gtacattgc cacacaagg accaaaaaa 
      aaacacaat tggtgattt ttggcgaat ggtttggca 
      agaaaacgt gagacatat cgtcatgtt ggccaacct 
      ctacgaaaa cgaaaagaa aaaagtgga gaaatattg 
      gcccgagat gggcgagaa tttacgtta tggtacaat 
      cacacttca atatttttc aaatcgagt ctatgcgga 
      ctacgagca tcgagtatt taaagtcac caatggcaa 
      tagtaccag ggaggtaca gcagtacca cttccagtc 
      atggcccga ccatggggt accacttta cgcccaaac 
      acttatccc atttttgga aaacctcca gaaaatccc 
      acttagtac caaatcacc gattgtagt ccactgcag 
      tgctggagt tggcagaac tggtactat aattttgtg 
      tgattcttg cttaagaat ggccgttag tgagaaatg 
      tgtagatgt gttgggaac acttcaaaa aattcgcaa 
      ccaaagacc caacatggt cgataatgt cgaacaata 
      caaactggc gcatttggt cattttgga ctatttgga 
      tggtatccg cactggaat accatgtag cgagattgg 
      agaagtgga agaattgtt aaaaagtga cgaattgaa 
      gaaacagat gcgttatct ggagactac agcttggca 
      agacgagat attcatgcc aacagtgtt acaaaatga 
      aacttcatt tgtggttaa aaaaaaaaa tcgcttcca 
      gaacattat tcccggcaa tcacaggta catcttctt 
      gcctcgtta tccaatcga tgacgaatc ttcaattta 
      cataaacgc ggttactgt cgatggttt caaacaccc 
      tgatcgttt tattgtgac acaacaacc gttaccaaa 
      caccctcgg agatttttg gcgcctggt ccacgataa 
      ggacatttc agtgatcat ttccttaaa cgaaatcga 
      cttaaacta cacgacttg caacttttg gccgtctga 
      agacaacaa agagctgaa tccggttga tttccttac 
      tctcaaaca cgccaaaac ccttatatg tgataccta 
      tgacgttat aaccgttca catgcatga tcaaaataa 
      tactgacca aaaagtgat cgaaatact tgcattaaa 
      aaattggcc ggcgatgaa tagctgtcc ggacaaaat 
      tgacagttt tttgacttt ttgggaaga gtcgtatgc 
      gacttccag gcagtctgg tcaagttat tgtcacttg 
      ctttgatgg agcacttgc gtccggtct gtacgtctc 
      catgtcgtt cattttgga aaaaattaa attagagca 
      agtttgtga cgtttgcca agctgtgag aattgtgag 
      acataatag gaaacaatt cgtcgagaa agaagaaca 
      atttatttt tttgtataa agctgcgat gacttatat 
      tggtggatt tgagattta tgctaattt taattagac 
      atactttaa ttatcgaac tgtacctaa attaattga 
      gtgaataaa ttttgtttg aaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY