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Gene information
General information
TC034766 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 2989293..2994087
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034766
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01582

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034766 RA
      MREIVHLQA GQCGNQVGA KFWEVISEE HGIDPSGIY 
      LGESDLQLE RINVYYNEA TVASRSNGG KYVPRAILL 
      DLEPGTMDA VRSGIYGRL FRPDNFVFG QSGAGNNWA 
      KGHYTEGAE LVDAVLDVV RKEAENCDC LQGFQLTHS 
      LGGGTGSGM GTLLISKIR EEYPDRIMN TYSVMPSPK 
      VSDTVVEPY NATLSVHQL VENTDETYC IDNEALYDI 
      CFRTLKVPN PSYGDLNHL VSLTMSGVT TCLRFPGQL 
      NADLRKLAV NMVPFPRLH FFMPGFAPL TSRGSQQYR 
      ALTVPELTQ QMFDAKNMM AACDPRHGR YLTVAAVFR 
      GRMSMKEVD EQMLAVQNK NSSYFVEWI PNNVKTAVC 
      DIPPVGLKM SSTFIGNTT AIQELFKRI SEQFAAMFR 
      RKAFLHWYT GEGMDEMEF TEAESNMND LVSEYQQYQ 
      EATADEEYE AEEEAAADD FNC
    CDS sequence
    • >TC034766 RA
      atgagggaa atcgtgcat cttcaagcc ggccaatgc 
      gggaaccaa gtcggggct aagttttgg gaagtgatt 
      tctgaagag cacggcatt gaccccagc ggaatctat 
      ttaggcgag agcgacttg caattagaa cgaatcaac 
      gtctactac aacgaagcc acagttgct tcgcgcagc 
      aatggtggc aagtacgtc ccccgtgcc atcctcctg 
      gacctggag cccggtacc atggacgcc gtccgctct 
      ggcatctac ggccgcctt ttccgtccc gacaatttc 
      gtctttggc caatccgga gccggaaac aactgggcc 
      aaaggccac tacaccgaa ggtgccgaa ctagtggac 
      gccgtccta gacgtggtg cgcaaagaa gccgaaaac 
      tgcgactgc ctccaaggc ttccagcta acacactca 
      cttggaggc ggcaccggc tccggcatg ggcacttta 
      ctcatctcc aaaatcagg gaggagtac cccgatagg 
      atcatgaac acgtattcg gtgatgcct tcaccgaaa 
      gtttccgac acggtcgtg gaaccgtac aatgccacc 
      ttatcagtg caccaactg gtcgaaaac actgatgag 
      acgtattgt atcgataac gaggcgctt tatgacatc 
      tgtttccgc actttgaaa gtcccgaac ccgagctac 
      ggcgacttg aaccatttg gtgtcgttg acaatgtcg 
      ggagtcacg acttgtctg cgattcccc ggtcaattg 
      aacgcagat ctgcgcaaa ttggcggtc aatatggtg 
      ccattcccg cgtttgcac ttcttcatg cctggtttt 
      gcgccctta acttccagg ggaagtcag caataccgt 
      gcgttgact gttcccgag ctcacgcag caaatgttc 
      gatgccaag aacatgatg gcggcgtgt gaccccaga 
      catgggagg tacttgaca gtggctgct gtttttaga 
      ggaagaatg tcgatgaaa gaagtcgac gaacaaatg 
      ctcgccgtt cagaataaa aatagcagt tatttcgtc 
      gagtggatt ccgaataat gtgaaaacg gccgtttgt 
      gacattcca ccagttggg ctaaaaatg tcatcgacg 
      ttcatcggg aacacgaca gcgatccag gagctgttc 
      aagcggatt tccgaacaa ttcgctgct atgtttaga 
      cgcaaagcg ttcttgcat tggtacacg ggggaagga 
      atggacgag atggaattc actgaggct gaatcaaac 
      atgaacgac ttggtctcg gagtaccaa cagtaccaa 
      gaggcaact gctgatgag gagtacgag gccgaggaa 
      gaggccgct gctgatgat ttcaattgt taa
    mRNA sequence
    • >TC034766 RA
      tttcaatca agatgaggg aaatcgtgc atcttcaag 
      ccggccaat gcgggaacc aagtcgggg ctaagtttt 
      gggaagtga tttctgaag agcacggca ttgacccca 
      gcggaatct atttaggcg agagcgact tgcaattag 
      aacgaatca acgtctact acaacgaag ccacagttg 
      cttcgcgca gcaatggtg gcaagtacg tcccccgtg 
      ccatcctcc tggacctgg agcccggta ccatggacg 
      ccgtccgct ctggcatct acggccgcc ttttccgtc 
      ccgacaatt tcgtctttg gccaatccg gagccggaa 
      acaactggg ccaaaggcc actacaccg aaggtgccg 
      aactagtgg acgccgtcc tagacgtgg tgcgcaaag 
      aagccgaaa actgcgact gcctccaag gcttccagc 
      taacacact cacttggag gcggcaccg gctccggca 
      tgggcactt tactcatct ccaaaatca gggaggagt 
      accccgata ggatcatga acacgtatt cggtgatgc 
      cttcaccga aagtttccg acacggtcg tggaaccgt 
      acaatgcca ccttatcag tgcaccaac tggtcgaaa 
      acactgatg agacgtatt gtatcgata acgaggcgc 
      tttatgaca tctgtttcc gcactttga aagtcccga 
      acccgagct acggcgact tgaaccatt tggtgtcgt 
      tgacaatgt cgggagtca cgacttgtc tgcgattcc 
      ccggtcaat tgaacgcag atctgcgca aattggcgg 
      tcaatatgg tgccattcc cgcgtttgc acttcttca 
      tgcctggtt ttgcgccct taacttcca ggggaagtc 
      agcaatacc gtgcgttga ctgttcccg agctcacgc 
      agcaaatgt tcgatgcca agaacatga tggcggcgt 
      gtgacccca gacatggga ggtacttga cagtggctg 
      ctgttttta gaggaagaa tgtcgatga aagaagtcg 
      acgaacaaa tgctcgccg ttcagaata aaaatagca 
      gttatttcg tcgagtgga ttccgaata atgtgaaaa 
      cggccgttt gtgacattc caccagttg ggctaaaaa 
      tgtcatcga cgttcatcg ggaacacga cagcgatcc 
      aggagctgt tcaagcgga tttccgaac aattcgctg 
      ctatgttta gacgcaaag cgttcttgc attggtaca 
      cgggggaag gaatggacg agatggaat tcactgagg 
      ctgaatcaa acatgaacg acttggtct cggagtacc 
      aacagtacc aagaggcaa ctgctgatg aggagtacg 
      aggccgagg aagaggccg ctgctgatg atttcaatt 
      gttaaaaaa taaccccca attgatatt tattgtagt 
      actaagact gaattttct a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY