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Gene information
General information
TC009584 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 2944770..2952053
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009584
    iBeetle screen iB_01581

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    abdomen partiallyremaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009584 RA
      MAKRLTEEM VIARTKISD LGKIKRLNC WGSELVDVS 
      LLRKMPCVE VLSLSINKI NSLIDFQFC KRLEELYIR 
      QNDIRDLNQ VVYLQNLPN LKNLWLGEN PCANSDGYR 
      FAVIKALPQ LQKLDNVQV TQEELREAQ RKGRTLCHP 
      DDAQDSEEE YSSPPQPPQ YYSESEYSP VQRSPPPRQ 
      EPDYEIEES EDLYPRDEV SEESKEYSP PRQPSPPRR 
      AEPPVPPVR RSYSPEPPT YVERRRNSY ERESPKQPP 
      QYYDQRCPA DHAFEDNTT HHCDNNYRE VKLVNSQSA 
      NCIKEYTNG DRYAHSQYR ESDSSAHSH RCPVAHRPP 
      FVRRPVTRN SNILSAVLR LVKELDYPS LEVVEMEVR 
      SRMDELDE
    • >TC009584 RB
      MAKRLTEEM VIARTKISD LGKIKRLNC WGSELVDVS 
      LLRKMPCVE VLSLSINKI NSLIDFQFC KRLEELYIR 
      QNDIRDLNQ VVYLQNLPN LKNLWLGEN PCANSDGYR 
      FAVIKALPQ LQKLDNVQV TQEELREAQ RKGRTLCHP 
      DDAQDSEEE YSSPPQPPQ YYSESEYSP VQRSPPPRQ 
      EPDYEIEES EDLYPRDEV SEESKEYSP PRQPSPPRR 
      AEPPVPPVR RSYSPEPPT YVERRRNSY ERESPKQPP 
      QYYDEYTNG DRYAHSQYR ESDSSAHSH RCPVAHRPP 
      FVRRPVTRN SNILSAVLR LVKELDYPS LEVVEMEVR 
      SRMDELDE
    • >TC009584 RC
      MAKRLTEEM VIARTKISD LGKIKRLNC WGSELVDVS 
      LLRKMPCVE VLSLSINKI NSLIDFQFC KRLEELYIR 
      QNDIRDLNQ VVYLQNLPN LKNLWLGEN PCANSDGYR 
      FAVIKALPQ LQKLDNVQV TQEELREAQ RKGRTLCHP 
      DDAQDSEEE YSSPPQPPQ YYSESEYSP VQRSPPPRQ 
      EPSPPRRAE PPVPPVRRS YSPEPPTYV ERRRNSYER 
      ESPKQPPQY YDQRCPADH AFEDNTTHH CDNNYREVK 
      LVNSQSANC IKEYTNGDR YAHSQYRES DSSAHSHRC 
      PVAHRPPFV RRPVTRNSN ILSAVLRLV KELDYPSLE 
      VVEMEVRSR MDELDE
    CDS sequence
    • >TC009584 RA
      atggcaaaa cgattaacc gaagaaatg gtcatcgcg 
      aggaccaaa atctccgac cttggtaaa atcaaaaga 
      ctcaactgt tggggaagt gaacttgtg gacgtctcc 
      ctgttgcgt aaaatgccc tgcgttgaa gttttatct 
      ctcagcatc aacaaaatt aacagcttg atcgacttc 
      caattttgc aaacgcctt gaggagctc tacatccgc 
      caaaatgac attcgcgat ttgaatcaa gttgtctat 
      ttgcaaaat ctccccaat ttgaagaat ctctggttg 
      ggggaaaac ccttgcgcc aattcagac ggctaccga 
      tttgccgta atcaaagcc ttgcctcag ctccaaaaa 
      ttggacaat gttcaagta acgcaagaa gaattgaga 
      gaagctcaa agaaaaggt cgaacgtta tgtcatcca 
      gatgacgct caagatagt gaagaggag tattcctcc 
      ccaccacaa cctcctcaa tactactct gaatcggag 
      tacagtcct gtacaaagg agtcctcca ccgagacaa 
      gagcccgac tatgaaatc gaagaaagt gaagatctg 
      tatccgaga gatgaggtt tcggaagaa agtaaagaa 
      tactcacca ccgagacag cccagcccc cctcgtcgg 
      gcggagccg cctgtgccc ccggtgcgt cgtagctac 
      tcaccggag ccccccacg tacgtagaa cgtcgtcgt 
      aacagctac gaacgcgaa tcgcccaaa cagccccca 
      caatattac gatcaaagg tgtcccgcc gatcacgca 
      ttcgaagat aacaccacc catcactgt gataataac 
      tatagggaa gtcaaactc gttaacagc caatcagct 
      aactgcatc aaggaatat acgaatggt gaccggtac 
      gcgcactcg caataccgg gagagtgac tcgtcggct 
      cacagccac cgatgtccg gtggcccat aggccccca 
      tttgtgcgg cgccccgtc acaaggaat tcgaatatt 
      ttatcggcg gttttgcgt ttggtgaag gagttggat 
      tatccaagt ttggaggtg gtggagatg gaagttagg 
      tcacggatg gacgaattg gatgaatga
    • >TC009584 RB
      atggcaaaa cgattaacc gaagaaatg gtcatcgcg 
      aggaccaaa atctccgac cttggtaaa atcaaaaga 
      ctcaactgt tggggaagt gaacttgtg gacgtctcc 
      ctgttgcgt aaaatgccc tgcgttgaa gttttatct 
      ctcagcatc aacaaaatt aacagcttg atcgacttc 
      caattttgc aaacgcctt gaggagctc tacatccgc 
      caaaatgac attcgcgat ttgaatcaa gttgtctat 
      ttgcaaaat ctccccaat ttgaagaat ctctggttg 
      ggggaaaac ccttgcgcc aattcagac ggctaccga 
      tttgccgta atcaaagcc ttgcctcag ctccaaaaa 
      ttggacaat gttcaagta acgcaagaa gaattgaga 
      gaagctcaa agaaaaggt cgaacgtta tgtcatcca 
      gatgacgct caagatagt gaagaggag tattcctcc 
      ccaccacaa cctcctcaa tactactct gaatcggag 
      tacagtcct gtacaaagg agtcctcca ccgagacaa 
      gagcccgac tatgaaatc gaagaaagt gaagatctg 
      tatccgaga gatgaggtt tcggaagaa agtaaagaa 
      tactcacca ccgagacag cccagcccc cctcgtcgg 
      gcggagccg cctgtgccc ccggtgcgt cgtagctac 
      tcaccggag ccccccacg tacgtagaa cgtcgtcgt 
      aacagctac gaacgcgaa tcgcccaaa cagccccca 
      caatattac gatgaatat acgaatggt gaccggtac 
      gcgcactcg caataccgg gagagtgac tcgtcggct 
      cacagccac cgatgtccg gtggcccat aggccccca 
      tttgtgcgg cgccccgtc acaaggaat tcgaatatt 
      ttatcggcg gttttgcgt ttggtgaag gagttggat 
      tatccaagt ttggaggtg gtggagatg gaagttagg 
      tcacggatg gacgaattg gatgaatga
    • >TC009584 RC
      atggcaaaa cgattaacc gaagaaatg gtcatcgcg 
      aggaccaaa atctccgac cttggtaaa atcaaaaga 
      ctcaactgt tggggaagt gaacttgtg gacgtctcc 
      ctgttgcgt aaaatgccc tgcgttgaa gttttatct 
      ctcagcatc aacaaaatt aacagcttg atcgacttc 
      caattttgc aaacgcctt gaggagctc tacatccgc 
      caaaatgac attcgcgat ttgaatcaa gttgtctat 
      ttgcaaaat ctccccaat ttgaagaat ctctggttg 
      ggggaaaac ccttgcgcc aattcagac ggctaccga 
      tttgccgta atcaaagcc ttgcctcag ctccaaaaa 
      ttggacaat gttcaagta acgcaagaa gaattgaga 
      gaagctcaa agaaaaggt cgaacgtta tgtcatcca 
      gatgacgct caagatagt gaagaggag tattcctcc 
      ccaccacaa cctcctcaa tactactct gaatcggag 
      tacagtcct gtacaaagg agtcctcca ccgagacaa 
      gagcccagc ccccctcgt cgggcggag ccgcctgtg 
      cccccggtg cgtcgtagc tactcaccg gagcccccc 
      acgtacgta gaacgtcgt cgtaacagc tacgaacgc 
      gaatcgccc aaacagccc ccacaatat tacgatcaa 
      aggtgtccc gccgatcac gcattcgaa gataacacc 
      acccatcac tgtgataat aactatagg gaagtcaaa 
      ctcgttaac agccaatca gctaactgc atcaaggaa 
      tatacgaat ggtgaccgg tacgcgcac tcgcaatac 
      cgggagagt gactcgtcg gctcacagc caccgatgt 
      ccggtggcc cataggccc ccatttgtg cggcgcccc 
      gtcacaagg aattcgaat attttatcg gcggttttg 
      cgtttggtg aaggagttg gattatcca agtttggag 
      gtggtggag atggaagtt aggtcacgg atggacgaa 
      ttggatgaa tga
    mRNA sequence
    • >TC009584 RA
      ccaaatttg acagttctt ggagaacca ccaaagctc 
      aaacatgtg cagtttttc gcttgtaat cgcttgtta 
      acccactat gtaaataaa cacagtgta attcaaaat 
      ggcaaaacg attaaccga agaaatggt catcgcgag 
      gaccaaaat ctccgacct tggtaaaat caaaagact 
      caactgttg gggaagtga acttgtgga cgtctccct 
      gttgcgtaa aatgccctg cgttgaagt tttatctct 
      cagcatcaa caaaattaa cagcttgat cgacttcca 
      attttgcaa acgccttga ggagctcta catccgcca 
      aaatgacat tcgcgattt gaatcaagt tgtctattt 
      gcaaaatct ccccaattt gaagaatct ctggttggg 
      ggaaaaccc ttgcgccaa ttcagacgg ctaccgatt 
      tgccgtaat caaagcctt gcctcagct ccaaaaatt 
      ggacaatgt tcaagtaac gcaagaaga attgagaga 
      agctcaaag aaaaggtcg aacgttatg tcatccaga 
      tgacgctca agatagtga agaggagta ttcctcccc 
      accacaacc tcctcaata ctactctga atcggagta 
      cagtcctgt acaaaggag tcctccacc gagacaaga 
      gcccgacta tgaaatcga agaaagtga agatctgta 
      tccgagaga tgaggtttc ggaagaaag taaagaata 
      ctcaccacc gagacagcc cagcccccc tcgtcgggc 
      ggagccgcc tgtgccccc ggtgcgtcg tagctactc 
      accggagcc ccccacgta cgtagaacg tcgtcgtaa 
      cagctacga acgcgaatc gcccaaaca gcccccaca 
      atattacga tcaaaggtg tcccgccga tcacgcatt 
      cgaagataa caccaccca tcactgtga taataacta 
      tagggaagt caaactcgt taacagcca atcagctaa 
      ctgcatcaa ggaatatac gaatggtga ccggtacgc 
      gcactcgca ataccggga gagtgactc gtcggctca 
      cagccaccg atgtccggt ggcccatag gcccccatt 
      tgtgcggcg ccccgtcac aaggaattc gaatatttt 
      atcggcggt tttgcgttt ggtgaagga gttggatta 
      tccaagttt ggaggtggt ggagatgga agttaggtc 
      acggatgga cgaattgga tgaatgacg aaaggagga 
      gtcgtcaaa agacgatac agcacaaga ggttacaga 
      aatctgagc tacgcacag caagtattg acttattta 
      ttgctaagt ttctgatcg aattttacc ggtcggaga 
      tttttactt atttaggat taatttttt acgttattg 
      ggtatctac ttaatgcag ctaatttga gtattacgt 
      acggtcgga aatgatcta aacaactaa ataaaagtc 
      tgatttttt ttctaaacg cattatcgc ataagcttc 
      atgtacata aactgccac gaatttaat gtaacaaca 
      atacaaggt ttttacaaa cgaaactat tttcaatgg 
      ttgttttgg ggtttcgta acacacttg ttgttggtt 
      gcaaatttg aatcgtttc attccctcc attagactg 
      ttattgttt aatgttata ttttatact gttaatcta 
      gtctttagt tgagtaact ttttcattt gtccccgac 
      gttaagtat taaagtaag tgcaatttg taaaactat 
      taataaatt aatattatt atc
    • >TC009584 RB
      ccaaatttg acagttctt ggagaacca ccaaagctc 
      aaacatgtg cagtttttc gcttgtaat cgcttgtta 
      acccactat gtaaataaa cacagtgta attcaaaat 
      ggcaaaacg attaaccga agaaatggt catcgcgag 
      gaccaaaat ctccgacct tggtaaaat caaaagact 
      caactgttg gggaagtga acttgtgga cgtctccct 
      gttgcgtaa aatgccctg cgttgaagt tttatctct 
      cagcatcaa caaaattaa cagcttgat cgacttcca 
      attttgcaa acgccttga ggagctcta catccgcca 
      aaatgacat tcgcgattt gaatcaagt tgtctattt 
      gcaaaatct ccccaattt gaagaatct ctggttggg 
      ggaaaaccc ttgcgccaa ttcagacgg ctaccgatt 
      tgccgtaat caaagcctt gcctcagct ccaaaaatt 
      ggacaatgt tcaagtaac gcaagaaga attgagaga 
      agctcaaag aaaaggtcg aacgttatg tcatccaga 
      tgacgctca agatagtga agaggagta ttcctcccc 
      accacaacc tcctcaata ctactctga atcggagta 
      cagtcctgt acaaaggag tcctccacc gagacaaga 
      gcccgacta tgaaatcga agaaagtga agatctgta 
      tccgagaga tgaggtttc ggaagaaag taaagaata 
      ctcaccacc gagacagcc cagcccccc tcgtcgggc 
      ggagccgcc tgtgccccc ggtgcgtcg tagctactc 
      accggagcc ccccacgta cgtagaacg tcgtcgtaa 
      cagctacga acgcgaatc gcccaaaca gcccccaca 
      atattacga tgaatatac gaatggtga ccggtacgc 
      gcactcgca ataccggga gagtgactc gtcggctca 
      cagccaccg atgtccggt ggcccatag gcccccatt 
      tgtgcggcg ccccgtcac aaggaattc gaatatttt 
      atcggcggt tttgcgttt ggtgaagga gttggatta 
      tccaagttt ggaggtggt ggagatgga agttaggtc 
      acggatgga cgaattgga tgaatgacg aaaggagga 
      gtcgtcaaa agacgatac agcacaaga ggttacaga 
      aatctgagc tacgcacag caagtattg acttattta 
      ttgctaagt ttctgatcg aattttacc ggtcggaga 
      tttttactt atttaggat taatttttt acgttattg 
      ggtatctac ttaatgcag ctaatttga gtattacgt 
      acggtcgga aatgatcta aacaactaa ataaaagtc 
      tgatttttt ttctaaacg cattatcgc ataagcttc 
      atgtacata aactgccac gaatttaat gtaacaaca 
      atacaaggt ttttacaaa cgaaactat tttcaatgg 
      ttgttttgg ggtttcgta acacacttg ttgttggtt 
      gcaaatttg aatcgtttc attccctcc attagactg 
      ttattgttt aatgttata ttttatact gttaatcta 
      gtctttagt tgagtaact ttttcattt gtccccgac 
      gttaagtat taaagtaag tgcaatttg taaaactat 
      taataaatt aatattatt atc
    • >TC009584 RC
      ccaaatttg acagttctt ggagaacca ccaaagctc 
      aaacatgtg cagtttttc gcttgtaat cgcttgtta 
      acccactat gtaaataaa cacagtgta attcaaaat 
      ggcaaaacg attaaccga agaaatggt catcgcgag 
      gaccaaaat ctccgacct tggtaaaat caaaagact 
      caactgttg gggaagtga acttgtgga cgtctccct 
      gttgcgtaa aatgccctg cgttgaagt tttatctct 
      cagcatcaa caaaattaa cagcttgat cgacttcca 
      attttgcaa acgccttga ggagctcta catccgcca 
      aaatgacat tcgcgattt gaatcaagt tgtctattt 
      gcaaaatct ccccaattt gaagaatct ctggttggg 
      ggaaaaccc ttgcgccaa ttcagacgg ctaccgatt 
      tgccgtaat caaagcctt gcctcagct ccaaaaatt 
      ggacaatgt tcaagtaac gcaagaaga attgagaga 
      agctcaaag aaaaggtcg aacgttatg tcatccaga 
      tgacgctca agatagtga agaggagta ttcctcccc 
      accacaacc tcctcaata ctactctga atcggagta 
      cagtcctgt acaaaggag tcctccacc gagacaaga 
      gcccagccc ccctcgtcg ggcggagcc gcctgtgcc 
      cccggtgcg tcgtagcta ctcaccgga gccccccac 
      gtacgtaga acgtcgtcg taacagcta cgaacgcga 
      atcgcccaa acagccccc acaatatta cgatcaaag 
      gtgtcccgc cgatcacgc attcgaaga taacaccac 
      ccatcactg tgataataa ctataggga agtcaaact 
      cgttaacag ccaatcagc taactgcat caaggaata 
      tacgaatgg tgaccggta cgcgcactc gcaataccg 
      ggagagtga ctcgtcggc tcacagcca ccgatgtcc 
      ggtggccca taggccccc atttgtgcg gcgccccgt 
      cacaaggaa ttcgaatat tttatcggc ggttttgcg 
      tttggtgaa ggagttgga ttatccaag tttggaggt 
      ggtggagat ggaagttag gtcacggat ggacgaatt 
      ggatgaatg acgaaagga ggagtcgtc aaaagacga 
      tacagcaca agaggttac agaaatctg agctacgca 
      cagcaagta ttgacttat ttattgcta agtttctga 
      tcgaatttt accggtcgg agattttta cttatttag 
      gattaattt tttacgtta ttgggtatc tacttaatg 
      cagctaatt tgagtatta cgtacggtc ggaaatgat 
      ctaaacaac taaataaaa gtctgattt tttttctaa 
      acgcattat cgcataagc ttcatgtac ataaactgc 
      cacgaattt aatgtaaca acaatacaa ggtttttac 
      aaacgaaac tattttcaa tggttgttt tggggtttc 
      gtaacacac ttgttgttg gttgcaaat ttgaatcgt 
      ttcattccc tccattaga ctgttattg tttaatgtt 
      atattttat actgttaat ctagtcttt agttgagta 
      actttttca tttgtcccc gacgttaag tattaaagt 
      aagtgcaat ttgtaaaac tattaataa attaatatt 
      attatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY