Status
Please wait ...
Gene information
General information
TC009561 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 2564359..2568621
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009561
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01579

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009561 RA
      MARISYNLL ELDVDKLFE EHKIEEIVE IEKLLDAEI 
      ERKRVELRS MVGDRYKDV LAASDAIKN MKTISQQIV 
      DNIQNITDI CEDLIESKD DKAAKTLPE IDTKCNEER 
      VLIVQVRLA IFMNEQIWT ALDGGDNLT AAQFYLLAQ 
      HIHMGLSLT KKEYLDKLP LLKQIRANL ATLRDQIFE 
      RIKQKLEFV EITAEETSS NLNALLLLR NQSATDLLN 
      IFIEHRKIA LNTVINTSH ASVRLQISA MVRCLITTI 
      HLLHDCFMC YGNSKKGLI WQQLENIVE DESIPTLSK 
      VDLPVTPLI AYIPDVIKQ FRPKYKSFV GESAPENVK 
      LGEWLASTK ESVCKGLES SLKLITSVK GLHIIREEA 
      LKIELPSDW ERICSESNL PENFNVWYY FFQSLITKR 
      ASELISKKV SCIIKDLES EIREVLSGN LGSEHGETD 
      LRWYTWVED PTDVSRIEN KHTGLSMKT LGYSQNVVN 
      LCEKLDGKY LELLIDVSQ YLYGKEFTD DVIIYPHLK 
      SDRKKFVDR EELENHLRL ESTKNSTSL TLFLHDFIK 
      SESHKVAKS LLCARFLQA VTNLCPHFN KCCTFNAST 
      EDWSRICGH FTKSSVQFW ENWIESCVQ ETDKQARKL 
      FSDVSVCSM ISVLLRWDS IEIQEQTDE KIFKSQIKV 
      PLKPSLTLH RLLSKLNDD LSHVLPHTL PKQVHLQFI 
      EKTTETILN HYKLALDHN QLNQNQSLQ LLFDVKFVT 
      TFSIQRENS KLVALSQEI CDKLRARID PFDLDVFYS 
      HLQNNVKRA VLQSQAILG CLLPSSAQL ANLGVGEKF 
      KEEKEPSVM ALSVPSVSF PLFCTFAHQ IPAQKAPVV 
      DVQEVKVSS PKLTKTTPK RSQDPTAAI RSGAAALFG 
      GLTTDWFS
    CDS sequence
    • >TC009561 RA
      atggcacgt atcagttac aatttactg gaattagac 
      gtcgacaaa ttgttcgaa gagcacaaa attgaagaa 
      atcgttgaa atagagaag ttacttgat gccgaaatt 
      gaacggaag cgggtggag ctccgctcc atggtcggc 
      gaccgctat aaagatgtc ctggccgcg tccgacgcc 
      atcaaaaac atgaaaaca atctcgcaa cagatcgta 
      gataacatt caaaacatc accgatatt tgcgaggat 
      ttgatcgaa agtaaggac gataaagcg gcaaaaaca 
      ctaccagag atagacacg aaatgtaac gaggagaga 
      gttttaatt gtacaagtc cggttagcg atttttatg 
      aatgagcaa atatggact gcactagat gggggcgat 
      aatttaaca gcggcacaa ttctattta ttggcacaa 
      cacatccac atgggctta agtttaact aagaaggaa 
      tatttagat aaattgcca ctcttgaag caaattcga 
      gccaatttg gcgactctc cgagaccag attttcgaa 
      cggattaag caaaagttg gagtttgtc gaaattaca 
      gccgaggaa actagcagc aatttaaac gctttgttg 
      ctcttgaga aaccagagc gccaccgat ctgctaaat 
      atttttatc gaacaccga aaaatagcg cttaatacg 
      gtcataaat acgtctcac gctagcgtg cggcttcag 
      ataagtgca atggtgcgg tgtctcatc accacaatt 
      catttacta cacgattgt ttcatgtgt tatggtaac 
      agtaaaaaa ggtttgatt tggcaacag cttgaaaac 
      atcgttgaa gatgagtca attccgacg ttatctaag 
      gttgatctg cctgttacg ccccttatt gcgtacatt 
      ccggacgta atcaagcaa tttcggccg aaatataag 
      agttttgtg ggcgagtcg gcccctgaa aatgttaaa 
      ttgggggaa tggttggcc agtacgaaa gaatcggtc 
      tgcaagggt ttagagagt tccttaaaa cttataact 
      agcgtaaaa gggttgcac attattcgg gaagaggcg 
      ttaaaaatt gaattgcca agcgattgg gagcggatt 
      tgttccgag tcaaatttg ccggagaat ttcaacgta 
      tggtattat ttcttccaa agtttaatc acgaaacgt 
      gccagtgaa ttgatttcg aaaaaagtt tcatgtatt 
      attaaagat ctagaaagc gaaattagg gaagttttg 
      agtggtaat ttagggtcg gagcatggt gagactgac 
      ttaaggtgg tacacctgg gtagaggac ccgactgac 
      gttagccgc attgaaaat aaacacacc ggtttgtca 
      atgaaaacc ttgggttac tcgcaaaac gtcgtaaat 
      ctttgcgag aaactggac ggcaaatat ttagaatta 
      ttgatagac gtgtctcag tacttgtac ggcaaagaa 
      tttactgat gacgtaatt atttatcca catttaaag 
      agtgatagg aaaaagttt gtcgataga gaagaacta 
      gagaatcac cttcgcctc gaaagtaca aaaaattca 
      acaagttta acgttattt ttgcacgat tttatcaaa 
      agtgagagt cacaaagtg gcaaagtcg ctgctttgt 
      gcccgattt ctgcaagca gtgacgaat ttatgtcca 
      cattttaac aaatgttgc acttttaat gcctcgacg 
      gaggactgg agccggatt tgtggccat ttcacaaag 
      tccagtgta cagttttgg gaaaattgg attgaaagt 
      tgtgtgcaa gaaaccgac aagcaggcg aggaaattg 
      ttcagcgac gtcagtgtt tgctcgatg atttctgtc 
      cttttgcgg tgggattcg atcgagatc caggagcaa 
      accgacgag aagatcttc aaatcgcag atcaaagtc 
      cccctcaag ccctccctg accctccac cgcctcctc 
      tccaaactg aacgacgac ctcagccac gtcctcccc 
      cacaccctc cccaagcag gtccacctc caattcatc 
      gaaaaaacc accgaaaca atcctcaac cactacaag 
      ctcgccctc gaccacaac caactcaac cagaaccag 
      tcccttcaa ctcctcttc gacgtgaaa ttcgtcacg 
      acgttcagt attcagcga gaaaattcg aaattagtt 
      gcgttgtcg caagaaatc tgtgataaa cttcgggcg 
      agaatcgac ccgtttgac ctcgacgtg ttctactcg 
      cacctgcag aacaacgtc aaaagggcc gttttgcag 
      tcgcaggcc attctgggg tgtcttttg cccagttcg 
      gcgcagttg gccaacttg ggggtgggc gagaagttt 
      aaagaggag aaggagccg tcggtgatg gctttgagc 
      gtcccttcg gtgtctttt ccgctgttt tgtacgttt 
      gcacaccag attccggca cagaaggct ccggttgtt 
      gacgtgcag gaggttaag gtttcttca ccgaagctg 
      acgaaaaca acaccaaaa aggtctcaa gaccccacg 
      gcagcaatt cgttcaggt gctgctgcg ttgtttggg 
      ggcttaaca accgactgg ttttcatag
    mRNA sequence
    • >TC009561 RA
      tatttggca agaagtttt taggttaga atggcacgt 
      atcagttac aatttactg gaattagac gtcgacaaa 
      ttgttcgaa gagcacaaa attgaagaa atcgttgaa 
      atagagaag ttacttgat gccgaaatt gaacggaag 
      cgggtggag ctccgctcc atggtcggc gaccgctat 
      aaagatgtc ctggccgcg tccgacgcc atcaaaaac 
      atgaaaaca atctcgcaa cagatcgta gataacatt 
      caaaacatc accgatatt tgcgaggat ttgatcgaa 
      agtaaggac gataaagcg gcaaaaaca ctaccagag 
      atagacacg aaatgtaac gaggagaga gttttaatt 
      gtacaagtc cggttagcg atttttatg aatgagcaa 
      atatggact gcactagat gggggcgat aatttaaca 
      gcggcacaa ttctattta ttggcacaa cacatccac 
      atgggctta agtttaact aagaaggaa tatttagat 
      aaattgcca ctcttgaag caaattcga gccaatttg 
      gcgactctc cgagaccag attttcgaa cggattaag 
      caaaagttg gagtttgtc gaaattaca gccgaggaa 
      actagcagc aatttaaac gctttgttg ctcttgaga 
      aaccagagc gccaccgat ctgctaaat atttttatc 
      gaacaccga aaaatagcg cttaatacg gtcataaat 
      acgtctcac gctagcgtg cggcttcag ataagtgca 
      atggtgcgg tgtctcatc accacaatt catttacta 
      cacgattgt ttcatgtgt tatggtaac agtaaaaaa 
      ggtttgatt tggcaacag cttgaaaac atcgttgaa 
      gatgagtca attccgacg ttatctaag gttgatctg 
      cctgttacg ccccttatt gcgtacatt ccggacgta 
      atcaagcaa tttcggccg aaatataag agttttgtg 
      ggcgagtcg gcccctgaa aatgttaaa ttgggggaa 
      tggttggcc agtacgaaa gaatcggtc tgcaagggt 
      ttagagagt tccttaaaa cttataact agcgtaaaa 
      gggttgcac attattcgg gaagaggcg ttaaaaatt 
      gaattgcca agcgattgg gagcggatt tgttccgag 
      tcaaatttg ccggagaat ttcaacgta tggtattat 
      ttcttccaa agtttaatc acgaaacgt gccagtgaa 
      ttgatttcg aaaaaagtt tcatgtatt attaaagat 
      ctagaaagc gaaattagg gaagttttg agtggtaat 
      ttagggtcg gagcatggt gagactgac ttaaggtgg 
      tacacctgg gtagaggac ccgactgac gttagccgc 
      attgaaaat aaacacacc ggtttgtca atgaaaacc 
      ttgggttac tcgcaaaac gtcgtaaat ctttgcgag 
      aaactggac ggcaaatat ttagaatta ttgatagac 
      gtgtctcag tacttgtac ggcaaagaa tttactgat 
      gacgtaatt atttatcca catttaaag agtgatagg 
      aaaaagttt gtcgataga gaagaacta gagaatcac 
      cttcgcctc gaaagtaca aaaaattca acaagttta 
      acgttattt ttgcacgat tttatcaaa agtgagagt 
      cacaaagtg gcaaagtcg ctgctttgt gcccgattt 
      ctgcaagca gtgacgaat ttatgtcca cattttaac 
      aaatgttgc acttttaat gcctcgacg gaggactgg 
      agccggatt tgtggccat ttcacaaag tccagtgta 
      cagttttgg gaaaattgg attgaaagt tgtgtgcaa 
      gaaaccgac aagcaggcg aggaaattg ttcagcgac 
      gtcagtgtt tgctcgatg atttctgtc cttttgcgg 
      tgggattcg atcgagatc caggagcaa accgacgag 
      aagatcttc aaatcgcag atcaaagtc cccctcaag 
      ccctccctg accctccac cgcctcctc tccaaactg 
      aacgacgac ctcagccac gtcctcccc cacaccctc 
      cccaagcag gtccacctc caattcatc gaaaaaacc 
      accgaaaca atcctcaac cactacaag ctcgccctc 
      gaccacaac caactcaac cagaaccag tcccttcaa 
      ctcctcttc gacgtgaaa ttcgtcacg acgttcagt 
      attcagcga gaaaattcg aaattagtt gcgttgtcg 
      caagaaatc tgtgataaa cttcgggcg agaatcgac 
      ccgtttgac ctcgacgtg ttctactcg cacctgcag 
      aacaacgtc aaaagggcc gttttgcag tcgcaggcc 
      attctgggg tgtcttttg cccagttcg gcgcagttg 
      gccaacttg ggggtgggc gagaagttt aaagaggag 
      aaggagccg tcggtgatg gctttgagc gtcccttcg 
      gtgtctttt ccgctgttt tgtacgttt gcacaccag 
      attccggca cagaaggct ccggttgtt gacgtgcag 
      gaggttaag gtttcttca ccgaagctg acgaaaaca 
      acaccaaaa aggtctcaa gaccccacg gcagcaatt 
      cgttcaggt gctgctgcg ttgtttggg ggcttaaca 
      accgactgg ttttcatag cagtgtact ctgatccac 
      aattatacg tttttatac ttgtaaata tgtactcat 
      aagccccaa aatggaaaa taaaatgtt taataaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY