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Gene information
General information
TC009559 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 2496226..2498396
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009559
    iBeetle screen iB_01578

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009559 RA
      MGSFLAEVL SSVEKMELQ QVNTKCPEV AALVEACKA 
      EVVSHLENI YVQFTERPR KNKALAGKI TKLEEDINM 
      LRTNLEEIT RTELAKSEC DIQKHTHAL VRAKTEISL 
      VATLFYIHE ALMRIVDLQ KNRSFFACL EEFQAMEEV 
      FEGLDLEEN LAVLQEMQL EFKTERSAF HLTLGNLFK 
      DRVVLQAGG TRSVVKVNN DQQLEDVLC TLYCDNNSV 
      TVLDKVTKF LWDSVFVPI VDTTTEIKV GQEEGFSVL 
      EVITVDPNK KSSYVEVFA NIKAVLVFL SDNFDVSLT 
      EDLSTLQYI GRDLRDNLS ELIIKRCLQ DTIPSTKQG 
      LEDFRRVIE ATEDLEKSL FQAKIFAED TTSILSYAC 
      NIDVLFINK KCQEYLASA QELMKKDLH DLVEVGESQ 
      TSLDNTQGF PRCGVSRSV TTLLKLMES ILVESQTLS 
      QKCAGRLLY TVKNISVLY VTFVPEHHK KLLQTIPQQ 
      VALFYNNCM FLAYTLCLW GETHLTKLP SVLEVESSF 
      DREVSQLRA VASDKFASC VKSQIMQIV EIMKESGLG 
      LKQTLEQVD AATEKCLRQ CLRQQELLK TVWLKVLPY 
      EVYNRTLGE ILNSLCEFL INAVVKFED ISSDAAEQL 
      VELFKMVQT RGPKLFSDA KEVALFVTS WYKVSELAF 
      VLNANLLDI NDRWADGKG PLGLQFKAV ELKQLVRAL 
      FQNTERRAA LLERIHD
    CDS sequence
    • >TC009559 RA
      atgggttcg ttcctagcc gaggtgtta tcatctgtt 
      gaaaaaatg gaactgcag caagtaaac accaagtgc 
      cccgaagtc gccgccctt gtggaggcc tgcaaggcc 
      gaggtggtg tcgcacctg gagaacatc tacgtgcag 
      tttacggag cggcccagg aagaacaaa gcgctggca 
      ggtaagatc actaagctg gaagaggac ataaacatg 
      ctacgaacc aatctggag gaaatcaca cgaacggaa 
      ctagcgaaa tctgagtgc gacatccag aagcacact 
      cacgcgctg gtacgggcc aagaccgag atctcgctc 
      gtggccact ctgttctac atccacgag gcgcttatg 
      cgaatcgtg gacttgcag aaaaaccgg tcatttttc 
      gcctgcttg gaggagttc caggcgatg gaggaggtg 
      ttcgaaggc ttggacctg gaggagaat ctggcggtg 
      ctgcaggag atgcagttg gagtttaaa accgagcgc 
      tcggccttc cacctgact ttgggcaac ctgttcaag 
      gaccgcgtg gtgctccaa gccggaggc accagaagt 
      gtggtgaaa gtaaacaat gaccagcaa cttgaggac 
      gttctgtgc acgttgtat tgcgacaac aattcggtc 
      acggtgctg gacaaagtg accaagttt ttgtgggat 
      tctgttttc gtgcccatt gttgacaca acgactgag 
      attaaggtg ggacaagag gagggtttc agtgtcctg 
      gaggtgatt acagttgac cctaacaaa aaatcctcg 
      tacgttgag gttttcgcc aacataaag gcggtcttg 
      gtgttttta tcggacaat ttcgatgtt agcttaacc 
      gaggacttg tccacgtta cagtacatt gggcgcgac 
      ctccgtgat aatttatcg gaacttatc atcaaacgg 
      tgccttcaa gacaccatc ccgtcgacc aagcaaggc 
      ttggaagac ttcagacgg gtgattgag gcgaccgag 
      gacttggag aagtcgctt ttccaggcg aaaatcttc 
      gcagaagac accacgtcc atcctgagc tatgcctgc 
      aatattgac gttttgttc atcaataag aagtgccag 
      gagtatttg gcttcggcg caagaattg atgaagaaa 
      gacttgcac gatttggtg gaagtcggg gaaagccag 
      acttcgctt gacaacacg caggggttt ccccgatgt 
      ggcgtctcg cgcagtgtc accaccttg ctaaaactg 
      atggaaagc atcttggtt gaatcacaa actttgtcg 
      caaaagtgc gccgggcgc ctcctctac acggtgaaa 
      aatatcagt gttttgtat gttacgttc gttcccgaa 
      caccacaaa aagttgctt caaactatc ccccaacag 
      gtcgcgctt ttttacaac aattgtatg ttcctggcg 
      tacactttg tgcctttgg ggggagacc cacttgact 
      aaacttccc tcagtgctc gaggtcgag tcgagtttt 
      gacagggaa gtgtcgcag ctgcgggcc gttgcgtcc 
      gataagttc gccagttgt gtcaagtct caaattatg 
      caaattgtg gaaattatg aaagagtcg ggtttgggg 
      ttgaaacag actctcgaa caggtggac gcggccact 
      gaaaagtgt ctcagacag tgtttgagg cagcaagag 
      ctgctcaaa acggtctgg ttgaaggtt ttgccgtat 
      gaggtttat aataggaca ctgggcgag attttgaac 
      tcgttgtgt gagtttttg atcaatgct gtggtcaag 
      tttgaggac atttcctca gatgcggcc gagcagttg 
      gttgagctg tttaagatg gtgcaaact agagggccg 
      aagttgttt agtgacgcc aaagaagtg gcgctgttt 
      gtgacgagt tggtataaa gtgagcgag ttggcgttc 
      gttttgaat gcgaatttg ttggatatt aatgatcgg 
      tgggcggat gggaaaggg ccgctgggg ctgcagttt 
      aaggcggtg gagttgaaa cagttggtt agggcgctg 
      tttcagaat acggagagg agggcggcg ttgctcgaa 
      aggatccac gactga
    mRNA sequence
    • >TC009559 RA
      atcgtgccc ccataaaaa atgggttcg ttcctagcc 
      gaggtgtta tcatctgtt gaaaaaatg gaactgcag 
      caagtaaac accaagtgc cccgaagtc gccgccctt 
      gtggaggcc tgcaaggcc gaggtggtg tcgcacctg 
      gagaacatc tacgtgcag tttacggag cggcccagg 
      aagaacaaa gcgctggca ggtaagatc actaagctg 
      gaagaggac ataaacatg ctacgaacc aatctggag 
      gaaatcaca cgaacggaa ctagcgaaa tctgagtgc 
      gacatccag aagcacact cacgcgctg gtacgggcc 
      aagaccgag atctcgctc gtggccact ctgttctac 
      atccacgag gcgcttatg cgaatcgtg gacttgcag 
      aaaaaccgg tcatttttc gcctgcttg gaggagttc 
      caggcgatg gaggaggtg ttcgaaggc ttggacctg 
      gaggagaat ctggcggtg ctgcaggag atgcagttg 
      gagtttaaa accgagcgc tcggccttc cacctgact 
      ttgggcaac ctgttcaag gaccgcgtg gtgctccaa 
      gccggaggc accagaagt gtggtgaaa gtaaacaat 
      gaccagcaa cttgaggac gttctgtgc acgttgtat 
      tgcgacaac aattcggtc acggtgctg gacaaagtg 
      accaagttt ttgtgggat tctgttttc gtgcccatt 
      gttgacaca acgactgag attaaggtg ggacaagag 
      gagggtttc agtgtcctg gaggtgatt acagttgac 
      cctaacaaa aaatcctcg tacgttgag gttttcgcc 
      aacataaag gcggtcttg gtgttttta tcggacaat 
      ttcgatgtt agcttaacc gaggacttg tccacgtta 
      cagtacatt gggcgcgac ctccgtgat aatttatcg 
      gaacttatc atcaaacgg tgccttcaa gacaccatc 
      ccgtcgacc aagcaaggc ttggaagac ttcagacgg 
      gtgattgag gcgaccgag gacttggag aagtcgctt 
      ttccaggcg aaaatcttc gcagaagac accacgtcc 
      atcctgagc tatgcctgc aatattgac gttttgttc 
      atcaataag aagtgccag gagtatttg gcttcggcg 
      caagaattg atgaagaaa gacttgcac gatttggtg 
      gaagtcggg gaaagccag acttcgctt gacaacacg 
      caggggttt ccccgatgt ggcgtctcg cgcagtgtc 
      accaccttg ctaaaactg atggaaagc atcttggtt 
      gaatcacaa actttgtcg caaaagtgc gccgggcgc 
      ctcctctac acggtgaaa aatatcagt gttttgtat 
      gttacgttc gttcccgaa caccacaaa aagttgctt 
      caaactatc ccccaacag gtcgcgctt ttttacaac 
      aattgtatg ttcctggcg tacactttg tgcctttgg 
      ggggagacc cacttgact aaacttccc tcagtgctc 
      gaggtcgag tcgagtttt gacagggaa gtgtcgcag 
      ctgcgggcc gttgcgtcc gataagttc gccagttgt 
      gtcaagtct caaattatg caaattgtg gaaattatg 
      aaagagtcg ggtttgggg ttgaaacag actctcgaa 
      caggtggac gcggccact gaaaagtgt ctcagacag 
      tgtttgagg cagcaagag ctgctcaaa acggtctgg 
      ttgaaggtt ttgccgtat gaggtttat aataggaca 
      ctgggcgag attttgaac tcgttgtgt gagtttttg 
      atcaatgct gtggtcaag tttgaggac atttcctca 
      gatgcggcc gagcagttg gttgagctg tttaagatg 
      gtgcaaact agagggccg aagttgttt agtgacgcc 
      aaagaagtg gcgctgttt gtgacgagt tggtataaa 
      gtgagcgag ttggcgttc gttttgaat gcgaatttg 
      ttggatatt aatgatcgg tgggcggat gggaaaggg 
      ccgctgggg ctgcagttt aaggcggtg gagttgaaa 
      cagttggtt agggcgctg tttcagaat acggagagg 
      agggcggcg ttgctcgaa aggatccac gactgattc 
      ttacattta tttgtaatt gttgaattt aataaatta 
      attaactat tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY