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Gene information
General information
TC009557 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 2489406..2492117
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009557
    iBeetle screen iB_01577

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    remarks: bad quality of preparation,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009557 RA
      MSRVFRLYG SFCATHPWE VIVAVVTLT TCLLTVDQK 
      KEPPAQPCP GCFEEQFKA VDVIVMTVI RCLAILYSS 
      HQFRNLQKL GSKYILGLA GLFIVFSSF VFTSTVLNF 
      LQIDLVDLK DALFFFLLL IDLSKAATL AQFALTASS 
      QEEISSKIA RGMSVLGPT ITLDTVVET LVIGVGTLS 
      GVHRLEMLS YFACLSVLV NYVIFMTFY PACLSLMLE 
      LARISNFYG EKQPIVSKM LLEENEKAN PAVQRVKLI 
      MSIGLTAVH VHSRWFVKD ESTDSEIIT NTDHQNCAE 
      DSVYGYLMK QFALSADHI VILILVVTL IIKFIFFEN 
      DDHLKQAID DEPPHVLKR QKCITKAES INEEKEVQT 
      NDEEIESLD ETNTRNLEE CLQIYRSDG ASKLSDKEV 
      ILLIDTKHL PAYNLEKAV KNQERGVKI RRQLIQSLA 
      KCESAFLNL PYKHYDYSK VMGACCENV IGYIPVPLG 
      VAGPLVLDG KQYYIPMST TEGCLIASA NRGCRAVEH 
      CGIKSRIVS DGMTRGPVV RFNSITKAS EVTLWLEKP 
      ENFLKVKEN FDSTSRFAR LSRITSRVA GRYLFLRFV 
      AETGDAMGM NMLSKGTEK ALFFVQRFF PEMEILSLS 
      GNYCADKKP AAVNWVEGR GKSVVCEAI VSGDVVSSV 
      LKSSTQVLV DLNTSKNMI GSAMAGSIG GFNAHAANI 
      VTAIFIATG QDPAQNIAS SNCITIMEP WGDNGQDLY 
      VSCTMPSIE IGTIGGGTI LPAQSACLD MLGVRGPNE 
      ESPGANSKQ LARIVCATV LAGELSLMA ALATGQLVK 
      SHLKYNRSY SEKDSQDCC KT
    CDS sequence
    • >TC009557 RA
      atgtcgcgc gtgttccgc ttgtacggc tcgttctgc 
      gccacccac ccgtgggag gtgatcgtg gccgtggtg 
      accctcacg acttgcctg ctcaccgtc gaccagaag 
      aaggagccc ccggcgcag ccctgcccc ggatgcttc 
      gaggagcag ttcaaggcg gtggacgtc atcgtcatg 
      accgtgatc cggtgcttg gccatcctg tacagctcg 
      caccaattc cgcaacttg caaaagctc ggctccaag 
      tacatcctc ggcctcgcc ggcctcttc atcgtgttc 
      tccagcttc gtgttcact tccactgtc ctgaatttc 
      ctccaaatc gatctggtg gatctgaag gacgcgctg 
      ttctttttc ctgttgttg attgacctg tccaaagcg 
      gccactttg gcccagttc gcgctgact gcctccagt 
      caggaggag ataagtagt aaaattgca aggggcatg 
      tctgtcctg gggcccact attacgctg gatacggtc 
      gtcgagact ctagtcatt ggggtgggg accctgtcg 
      ggggtccac cgcttggag atgctttcg tattttgcg 
      tgtctttcc gttttagtc aattatgtt attttcatg 
      acgttctac cccgcgtgt ttgtcgcta atgttggag 
      ttggcccga atctcgaat ttctacggg gaaaagcaa 
      ccaattgtc tcgaaaatg ctgctcgaa gagaacgaa 
      aaagcaaat ccagctgta caacgtgtt aaactcata 
      atgtcaatc ggactcact gctgtgcac gtccacagt 
      cgttggttc gtcaaagac gaaagcaca gacagcgaa 
      attatcaca aacaccgac caccaaaat tgtgccgaa 
      gactcagtt tacggttat ttgatgaaa cagttcgct 
      ctgagtgcc gaccacatc gtaatccta atcctggtg 
      gtgaccctg atcatcaaa ttcatattt ttcgaaaac 
      gacgaccac ttgaaacaa gctatagac gatgagcca 
      cctcacgtc ctcaaacgc caaaaatgc atcaccaaa 
      gccgagtca ataaacgag gagaaggaa gttcaaacc 
      aacgacgaa gaaatcgaa agtttggac gagacaaac 
      acccgaaac ctcgaagag tgtctccag atttaccgc 
      tcggacgga gcgtccaaa cttagcgat aaggaagtg 
      atcctactc attgacacc aaacacttg ccggcctac 
      aatttggaa aaagcggtc aagaatcaa gaacgcggg 
      gttaaaatc cggcgccaa ctgatccaa agtttggcc 
      aaatgcgag tcagcgttt ctcaacctc ccgtataaa 
      cactatgac tatagtaaa gtcatgggt gcgtgttgc 
      gagaacgtg attggatac attcccgta ccattgggc 
      gtggctggg cctttggta cttgatggt aaacaatac 
      tacatcccc atgtcgacc actgagggt tgcctgatt 
      gccagcgcc aatcggggc tgtcgtgcg gtggaacat 
      tgcggtatt aagagccga atcgtttcc gacggaatg 
      actcgaggg cctgtcgtt cgttttaat tccataacg 
      aaggcgtcc gaagtcacg ctctggttg gaaaaaccg 
      gagaatttc ctcaaagtc aaggaaaat ttcgattcg 
      acgagtcgt ttcgcgcgt ttgtcgcgg attacaagc 
      cgggttgct ggtagatac ctgtttttg cggttcgtg 
      gccgagacg ggtgatgcc atgggcatg aatatgctg 
      tcgaaaggg acggaaaaa gcgctgttt ttcgtccag 
      aggttcttc cccgagatg gagattctg agtttgagt 
      gggaattac tgcgcggat aagaagcca gccgctgtg 
      aattgggtc gaaggccgg gggaagtcc gtggtttgt 
      gaagcgata gtttcgggt gatgtcgtt tcgtcagta 
      ttaaagtcg tcgacgcag gtcttggtc gatttgaac 
      acttccaag aatatgatc gggtcggcc atggccggg 
      agtattggg gggtttaat gcccacgct gccaacatc 
      gtgaccgcg attttcatc gcaactggc caagacccc 
      gctcagaac atagccagt agtaactgt attaccata 
      atggagccg tggggcgac aatgggcaa gatttgtac 
      gtttcttgc accatgcca tccatcgaa atcggtacc 
      attggcggt ggtaccatc cttccagcg cagtctgcg 
      tgtttggat atgttgggt gtgcggggg cccaacgag 
      gagtcccca ggagccaac tccaaacaa ctggcgagg 
      attgtctgt gcgacagtt ctggcaggg gagttgtcg 
      ctaatggcg gctttagcg acaggacag ttagttaaa 
      agtcactta aagtacaat cgttcgtat tcagagaag 
      gattctcag gattgttgt aaaacttag
    mRNA sequence
    • >TC009557 RA
      ctccaaaaa ataaaatgt tgtgttaag tgtgtaaaa 
      tgcccccga acggcgatg tcgcgcgtg ttccgcttg 
      tacggctcg ttctgcgcc acccacccg tgggaggtg 
      atcgtggcc gtggtgacc ctcacgact tgcctgctc 
      accgtcgac cagaagaag gagcccccg gcgcagccc 
      tgccccgga tgcttcgag gagcagttc aaggcggtg 
      gacgtcatc gtcatgacc gtgatccgg tgcttggcc 
      atcctgtac agctcgcac caattccgc aacttgcaa 
      aagctcggc tccaagtac atcctcggc ctcgccggc 
      ctcttcatc gtgttctcc agcttcgtg ttcacttcc 
      actgtcctg aatttcctc caaatcgat ctggtggat 
      ctgaaggac gcgctgttc tttttcctg ttgttgatt 
      gacctgtcc aaagcggcc actttggcc cagttcgcg 
      ctgactgcc tccagtcag gaggagata agtagtaaa 
      attgcaagg ggcatgtct gtcctgggg cccactatt 
      acgctggat acggtcgtc gagactcta gtcattggg 
      gtggggacc ctgtcgggg gtccaccgc ttggagatg 
      ctttcgtat tttgcgtgt ctttccgtt ttagtcaat 
      tatgttatt ttcatgacg ttctacccc gcgtgtttg 
      tcgctaatg ttggagttg gcccgaatc tcgaatttc 
      tacggggaa aagcaacca attgtctcg aaaatgctg 
      ctcgaagag aacgaaaaa gcaaatcca gctgtacaa 
      cgtgttaaa ctcataatg tcaatcgga ctcactgct 
      gtgcacgtc cacagtcgt tggttcgtc aaagacgaa 
      agcacagac agcgaaatt atcacaaac accgaccac 
      caaaattgt gccgaagac tcagtttac ggttatttg 
      atgaaacag ttcgctctg agtgccgac cacatcgta 
      atcctaatc ctggtggtg accctgatc atcaaattc 
      atatttttc gaaaacgac gaccacttg aaacaagct 
      atagacgat gagccacct cacgtcctc aaacgccaa 
      aaatgcatc accaaagcc gagtcaata aacgaggag 
      aaggaagtt caaaccaac gacgaagaa atcgaaagt 
      ttggacgag acaaacacc cgaaacctc gaagagtgt 
      ctccagatt taccgctcg gacggagcg tccaaactt 
      agcgataag gaagtgatc ctactcatt gacaccaaa 
      cacttgccg gcctacaat ttggaaaaa gcggtcaag 
      aatcaagaa cgcggggtt aaaatccgg cgccaactg 
      atccaaagt ttggccaaa tgcgagtca gcgtttctc 
      aacctcccg tataaacac tatgactat agtaaagtc 
      atgggtgcg tgttgcgag aacgtgatt ggatacatt 
      cccgtacca ttgggcgtg gctgggcct ttggtactt 
      gatggtaaa caatactac atccccatg tcgaccact 
      gagggttgc ctgattgcc agcgccaat cggggctgt 
      cgtgcggtg gaacattgc ggtattaag agccgaatc 
      gtttccgac ggaatgact cgagggcct gtcgttcgt 
      tttaattcc ataacgaag gcgtccgaa gtcacgctc 
      tggttggaa aaaccggag aatttcctc aaagtcaag 
      gaaaatttc gattcgacg agtcgtttc gcgcgtttg 
      tcgcggatt acaagccgg gttgctggt agatacctg 
      tttttgcgg ttcgtggcc gagacgggt gatgccatg 
      ggcatgaat atgctgtcg aaagggacg gaaaaagcg 
      ctgtttttc gtccagagg ttcttcccc gagatggag 
      attctgagt ttgagtggg aattactgc gcggataag 
      aagccagcc gctgtgaat tgggtcgaa ggccggggg 
      aagtccgtg gtttgtgaa gcgatagtt tcgggtgat 
      gtcgtttcg tcagtatta aagtcgtcg acgcaggtc 
      ttggtcgat ttgaacact tccaagaat atgatcggg 
      tcggccatg gccgggagt attgggggg tttaatgcc 
      cacgctgcc aacatcgtg accgcgatt ttcatcgca 
      actggccaa gaccccgct cagaacata gccagtagt 
      aactgtatt accataatg gagccgtgg ggcgacaat 
      gggcaagat ttgtacgtt tcttgcacc atgccatcc 
      atcgaaatc ggtaccatt ggcggtggt accatcctt 
      ccagcgcag tctgcgtgt ttggatatg ttgggtgtg 
      cgggggccc aacgaggag tccccagga gccaactcc 
      aaacaactg gcgaggatt gtctgtgcg acagttctg 
      gcaggggag ttgtcgcta atggcggct ttagcgaca 
      ggacagtta gttaaaagt cacttaaag tacaatcgt 
      tcgtattca gagaaggat tctcaggat tgttgtaaa 
      acttagatt taataaata gtgaaaagt ata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY