Status
Please wait ...
Gene information
General information
TC009557 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 2489406..2492117
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009557
    iBeetle screen iB_01577

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
     remarks: bad quality of preparation,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009557 RA
      MSRVFRLYG SFCATHPWE VIVAVVTLT TCLLTVDQK 
KEPPAQPCP GCFEEQFKA VDVIVMTVI RCLAILYSS 
HQFRNLQKL GSKYILGLA GLFIVFSSF VFTSTVLNF 
LQIDLVDLK DALFFFLLL IDLSKAATL AQFALTASS 
QEEISSKIA RGMSVLGPT ITLDTVVET LVIGVGTLS 
GVHRLEMLS YFACLSVLV NYVIFMTFY PACLSLMLE 
LARISNFYG EKQPIVSKM LLEENEKAN PAVQRVKLI 
MSIGLTAVH VHSRWFVKD ESTDSEIIT NTDHQNCAE 
DSVYGYLMK QFALSADHI VILILVVTL IIKFIFFEN 
DDHLKQAID DEPPHVLKR QKCITKAES INEEKEVQT 
NDEEIESLD ETNTRNLEE CLQIYRSDG ASKLSDKEV 
ILLIDTKHL PAYNLEKAV KNQERGVKI RRQLIQSLA 
KCESAFLNL PYKHYDYSK VMGACCENV IGYIPVPLG 
VAGPLVLDG KQYYIPMST TEGCLIASA NRGCRAVEH 
CGIKSRIVS DGMTRGPVV RFNSITKAS EVTLWLEKP 
ENFLKVKEN FDSTSRFAR LSRITSRVA GRYLFLRFV 
AETGDAMGM NMLSKGTEK ALFFVQRFF PEMEILSLS 
GNYCADKKP AAVNWVEGR GKSVVCEAI VSGDVVSSV 
LKSSTQVLV DLNTSKNMI GSAMAGSIG GFNAHAANI 
VTAIFIATG QDPAQNIAS SNCITIMEP WGDNGQDLY 
VSCTMPSIE IGTIGGGTI LPAQSACLD MLGVRGPNE 
ESPGANSKQ LARIVCATV LAGELSLMA ALATGQLVK 
SHLKYNRSY SEKDSQDCC KT
    CDS sequence
    • >TC009557 RA
      atgtcgcgc gtgttccgc ttgtacggc tcgttctgc 
gccacccac ccgtgggag gtgatcgtg gccgtggtg 
accctcacg acttgcctg ctcaccgtc gaccagaag 
aaggagccc ccggcgcag ccctgcccc ggatgcttc 
gaggagcag ttcaaggcg gtggacgtc atcgtcatg 
accgtgatc cggtgcttg gccatcctg tacagctcg 
caccaattc cgcaacttg caaaagctc ggctccaag 
tacatcctc ggcctcgcc ggcctcttc atcgtgttc 
tccagcttc gtgttcact tccactgtc ctgaatttc 
ctccaaatc gatctggtg gatctgaag gacgcgctg 
ttctttttc ctgttgttg attgacctg tccaaagcg 
gccactttg gcccagttc gcgctgact gcctccagt 
caggaggag ataagtagt aaaattgca aggggcatg 
tctgtcctg gggcccact attacgctg gatacggtc 
gtcgagact ctagtcatt ggggtgggg accctgtcg 
ggggtccac cgcttggag atgctttcg tattttgcg 
tgtctttcc gttttagtc aattatgtt attttcatg 
acgttctac cccgcgtgt ttgtcgcta atgttggag 
ttggcccga atctcgaat ttctacggg gaaaagcaa 
ccaattgtc tcgaaaatg ctgctcgaa gagaacgaa 
aaagcaaat ccagctgta caacgtgtt aaactcata 
atgtcaatc ggactcact gctgtgcac gtccacagt 
cgttggttc gtcaaagac gaaagcaca gacagcgaa 
attatcaca aacaccgac caccaaaat tgtgccgaa 
gactcagtt tacggttat ttgatgaaa cagttcgct 
ctgagtgcc gaccacatc gtaatccta atcctggtg 
gtgaccctg atcatcaaa ttcatattt ttcgaaaac 
gacgaccac ttgaaacaa gctatagac gatgagcca 
cctcacgtc ctcaaacgc caaaaatgc atcaccaaa 
gccgagtca ataaacgag gagaaggaa gttcaaacc 
aacgacgaa gaaatcgaa agtttggac gagacaaac 
acccgaaac ctcgaagag tgtctccag atttaccgc 
tcggacgga gcgtccaaa cttagcgat aaggaagtg 
atcctactc attgacacc aaacacttg ccggcctac 
aatttggaa aaagcggtc aagaatcaa gaacgcggg 
gttaaaatc cggcgccaa ctgatccaa agtttggcc 
aaatgcgag tcagcgttt ctcaacctc ccgtataaa 
cactatgac tatagtaaa gtcatgggt gcgtgttgc 
gagaacgtg attggatac attcccgta ccattgggc 
gtggctggg cctttggta cttgatggt aaacaatac 
tacatcccc atgtcgacc actgagggt tgcctgatt 
gccagcgcc aatcggggc tgtcgtgcg gtggaacat 
tgcggtatt aagagccga atcgtttcc gacggaatg 
actcgaggg cctgtcgtt cgttttaat tccataacg 
aaggcgtcc gaagtcacg ctctggttg gaaaaaccg 
gagaatttc ctcaaagtc aaggaaaat ttcgattcg 
acgagtcgt ttcgcgcgt ttgtcgcgg attacaagc 
cgggttgct ggtagatac ctgtttttg cggttcgtg 
gccgagacg ggtgatgcc atgggcatg aatatgctg 
tcgaaaggg acggaaaaa gcgctgttt ttcgtccag 
aggttcttc cccgagatg gagattctg agtttgagt 
gggaattac tgcgcggat aagaagcca gccgctgtg 
aattgggtc gaaggccgg gggaagtcc gtggtttgt 
gaagcgata gtttcgggt gatgtcgtt tcgtcagta 
ttaaagtcg tcgacgcag gtcttggtc gatttgaac 
acttccaag aatatgatc gggtcggcc atggccggg 
agtattggg gggtttaat gcccacgct gccaacatc 
gtgaccgcg attttcatc gcaactggc caagacccc 
gctcagaac atagccagt agtaactgt attaccata 
atggagccg tggggcgac aatgggcaa gatttgtac 
gtttcttgc accatgcca tccatcgaa atcggtacc 
attggcggt ggtaccatc cttccagcg cagtctgcg 
tgtttggat atgttgggt gtgcggggg cccaacgag 
gagtcccca ggagccaac tccaaacaa ctggcgagg 
attgtctgt gcgacagtt ctggcaggg gagttgtcg 
ctaatggcg gctttagcg acaggacag ttagttaaa 
agtcactta aagtacaat cgttcgtat tcagagaag 
gattctcag gattgttgt aaaacttag
    mRNA sequence
    • >TC009557 RA
      ctccaaaaa ataaaatgt tgtgttaag tgtgtaaaa 
tgcccccga acggcgatg tcgcgcgtg ttccgcttg 
tacggctcg ttctgcgcc acccacccg tgggaggtg 
atcgtggcc gtggtgacc ctcacgact tgcctgctc 
accgtcgac cagaagaag gagcccccg gcgcagccc 
tgccccgga tgcttcgag gagcagttc aaggcggtg 
gacgtcatc gtcatgacc gtgatccgg tgcttggcc 
atcctgtac agctcgcac caattccgc aacttgcaa 
aagctcggc tccaagtac atcctcggc ctcgccggc 
ctcttcatc gtgttctcc agcttcgtg ttcacttcc 
actgtcctg aatttcctc caaatcgat ctggtggat 
ctgaaggac gcgctgttc tttttcctg ttgttgatt 
gacctgtcc aaagcggcc actttggcc cagttcgcg 
ctgactgcc tccagtcag gaggagata agtagtaaa 
attgcaagg ggcatgtct gtcctgggg cccactatt 
acgctggat acggtcgtc gagactcta gtcattggg 
gtggggacc ctgtcgggg gtccaccgc ttggagatg 
ctttcgtat tttgcgtgt ctttccgtt ttagtcaat 
tatgttatt ttcatgacg ttctacccc gcgtgtttg 
tcgctaatg ttggagttg gcccgaatc tcgaatttc 
tacggggaa aagcaacca attgtctcg aaaatgctg 
ctcgaagag aacgaaaaa gcaaatcca gctgtacaa 
cgtgttaaa ctcataatg tcaatcgga ctcactgct 
gtgcacgtc cacagtcgt tggttcgtc aaagacgaa 
agcacagac agcgaaatt atcacaaac accgaccac 
caaaattgt gccgaagac tcagtttac ggttatttg 
atgaaacag ttcgctctg agtgccgac cacatcgta 
atcctaatc ctggtggtg accctgatc atcaaattc 
atatttttc gaaaacgac gaccacttg aaacaagct 
atagacgat gagccacct cacgtcctc aaacgccaa 
aaatgcatc accaaagcc gagtcaata aacgaggag 
aaggaagtt caaaccaac gacgaagaa atcgaaagt 
ttggacgag acaaacacc cgaaacctc gaagagtgt 
ctccagatt taccgctcg gacggagcg tccaaactt 
agcgataag gaagtgatc ctactcatt gacaccaaa 
cacttgccg gcctacaat ttggaaaaa gcggtcaag 
aatcaagaa cgcggggtt aaaatccgg cgccaactg 
atccaaagt ttggccaaa tgcgagtca gcgtttctc 
aacctcccg tataaacac tatgactat agtaaagtc 
atgggtgcg tgttgcgag aacgtgatt ggatacatt 
cccgtacca ttgggcgtg gctgggcct ttggtactt 
gatggtaaa caatactac atccccatg tcgaccact 
gagggttgc ctgattgcc agcgccaat cggggctgt 
cgtgcggtg gaacattgc ggtattaag agccgaatc 
gtttccgac ggaatgact cgagggcct gtcgttcgt 
tttaattcc ataacgaag gcgtccgaa gtcacgctc 
tggttggaa aaaccggag aatttcctc aaagtcaag 
gaaaatttc gattcgacg agtcgtttc gcgcgtttg 
tcgcggatt acaagccgg gttgctggt agatacctg 
tttttgcgg ttcgtggcc gagacgggt gatgccatg 
ggcatgaat atgctgtcg aaagggacg gaaaaagcg 
ctgtttttc gtccagagg ttcttcccc gagatggag 
attctgagt ttgagtggg aattactgc gcggataag 
aagccagcc gctgtgaat tgggtcgaa ggccggggg 
aagtccgtg gtttgtgaa gcgatagtt tcgggtgat 
gtcgtttcg tcagtatta aagtcgtcg acgcaggtc 
ttggtcgat ttgaacact tccaagaat atgatcggg 
tcggccatg gccgggagt attgggggg tttaatgcc 
cacgctgcc aacatcgtg accgcgatt ttcatcgca 
actggccaa gaccccgct cagaacata gccagtagt 
aactgtatt accataatg gagccgtgg ggcgacaat 
gggcaagat ttgtacgtt tcttgcacc atgccatcc 
atcgaaatc ggtaccatt ggcggtggt accatcctt 
ccagcgcag tctgcgtgt ttggatatg ttgggtgtg 
cgggggccc aacgaggag tccccagga gccaactcc 
aaacaactg gcgaggatt gtctgtgcg acagttctg 
gcaggggag ttgtcgcta atggcggct ttagcgaca 
ggacagtta gttaaaagt cacttaaag tacaatcgt 
tcgtattca gagaaggat tctcaggat tgttgtaaa 
acttagatt taataaata gtgaaaagt ata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY