| General information | |||
| TC009557 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
scaffold_seqid47: 407506..410217 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC009557 | |
| General information | ||||
| iB_01577 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)remarks: bad quality of preparation,
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC009557 RA
MSRVFRLYG SFCATHPWE VIVAVVTLT TCLLTVDQK KEPPAQPCP GCFEEQFKA VDVIVMTVI RCLAILYSS HQFRNLQKL GSKYILGLA GLFIVFSSF VFTSTVLNF LQIDLVDLK DALFFFLLL IDLSKAATL AQFALTASS QEEISSKIA RGMSVLGPT ITLDTVVET LVIGVGTLS GVHRLEMLS YFACLSVLV NYVIFMTFY PACLSLMLE LARISNFYG EKQPIVSKM LLEENEKAN PAVQRVKLI MSIGLTAVH VHSRWFVKD ESTDSEIIT NTDHQNCAE DSVYGYLMK QFALSADHI VILILVVTL IIKFIFFEN DDHLKQAID DEPPHVLKR QKCITKAES INEEKEVQT NDEEIESLD ETNTRNLEE CLQIYRSDG ASKLSDKEV ILLIDTKHL PAYNLEKAV KNQERGVKI RRQLIQSLA KCESAFLNL PYKHYDYSK VMGACCENV IGYIPVPLG VAGPLVLDG KQYYIPMST TEGCLIASA NRGCRAVEH CGIKSRIVS DGMTRGPVV RFNSITKAS EVTLWLEKP ENFLKVKEN FDSTSRFAR LSRITSRVA GRYLFLRFV AETGDAMGM NMLSKGTEK ALFFVQRFF PEMEILSLS GNYCADKKP AAVNWVEGR GKSVVCEAI VSGDVVSSV LKSSTQVLV DLNTSKNMI GSAMAGSIG GFNAHAANI VTAIFIATG QDPAQNIAS SNCITIMEP WGDNGQDLY VSCTMPSIE IGTIGGGTI LPAQSACLD MLGVRGPNE ESPGANSKQ LARIVCATV LAGELSLMA ALATGQLVK SHLKYNRSY SEKDSQDCC KT
- >TC009557 RA
atgtcgcgc gtgttccgc ttgtacggc tcgttctgc gccacccac ccgtgggag gtgatcgtg gccgtggtg accctcacg acttgcctg ctcaccgtc gaccagaag aaggagccc ccggcgcag ccctgcccc ggatgcttc gaggagcag ttcaaggcg gtggacgtc atcgtcatg accgtgatc cggtgcttg gccatcctg tacagctcg caccaattc cgcaacttg caaaagctc ggctccaag tacatcctc ggcctcgcc ggcctcttc atcgtgttc tccagcttc gtgttcact tccactgtc ctgaatttc ctccaaatc gatctggtg gatctgaag gacgcgctg ttctttttc ctgttgttg attgacctg tccaaagcg gccactttg gcccagttc gcgctgact gcctccagt caggaggag ataagtagt aaaattgca aggggcatg tctgtcctg gggcccact attacgctg gatacggtc gtcgagact ctagtcatt ggggtgggg accctgtcg ggggtccac cgcttggag atgctttcg tattttgcg tgtctttcc gttttagtc aattatgtt attttcatg acgttctac cccgcgtgt ttgtcgcta atgttggag ttggcccga atctcgaat ttctacggg gaaaagcaa ccaattgtc tcgaaaatg ctgctcgaa gagaacgaa aaagcaaat ccagctgta caacgtgtt aaactcata atgtcaatc ggactcact gctgtgcac gtccacagt cgttggttc gtcaaagac gaaagcaca gacagcgaa attatcaca aacaccgac caccaaaat tgtgccgaa gactcagtt tacggttat ttgatgaaa cagttcgct ctgagtgcc gaccacatc gtaatccta atcctggtg gtgaccctg atcatcaaa ttcatattt ttcgaaaac gacgaccac ttgaaacaa gctatagac gatgagcca cctcacgtc ctcaaacgc caaaaatgc atcaccaaa gccgagtca ataaacgag gagaaggaa gttcaaacc aacgacgaa gaaatcgaa agtttggac gagacaaac acccgaaac ctcgaagag tgtctccag atttaccgc tcggacgga gcgtccaaa cttagcgat aaggaagtg atcctactc attgacacc aaacacttg ccggcctac aatttggaa aaagcggtc aagaatcaa gaacgcggg gttaaaatc cggcgccaa ctgatccaa agtttggcc aaatgcgag tcagcgttt ctcaacctc ccgtataaa cactatgac tatagtaaa gtcatgggt gcgtgttgc gagaacgtg attggatac attcccgta ccattgggc gtggctggg cctttggta cttgatggt aaacaatac tacatcccc atgtcgacc actgagggt tgcctgatt gccagcgcc aatcggggc tgtcgtgcg gtggaacat tgcggtatt aagagccga atcgtttcc gacggaatg actcgaggg cctgtcgtt cgttttaat tccataacg aaggcgtcc gaagtcacg ctctggttg gaaaaaccg gagaatttc ctcaaagtc aaggaaaat ttcgattcg acgagtcgt ttcgcgcgt ttgtcgcgg attacaagc cgggttgct ggtagatac ctgtttttg cggttcgtg gccgagacg ggtgatgcc atgggcatg aatatgctg tcgaaaggg acggaaaaa gcgctgttt ttcgtccag aggttcttc cccgagatg gagattctg agtttgagt gggaattac tgcgcggat aagaagcca gccgctgtg aattgggtc gaaggccgg gggaagtcc gtggtttgt gaagcgata gtttcgggt gatgtcgtt tcgtcagta ttaaagtcg tcgacgcag gtcttggtc gatttgaac acttccaag aatatgatc gggtcggcc atggccggg agtattggg gggtttaat gcccacgct gccaacatc gtgaccgcg attttcatc gcaactggc caagacccc gctcagaac atagccagt agtaactgt attaccata atggagccg tggggcgac aatgggcaa gatttgtac gtttcttgc accatgcca tccatcgaa atcggtacc attggcggt ggtaccatc cttccagcg cagtctgcg tgtttggat atgttgggt gtgcggggg cccaacgag gagtcccca ggagccaac tccaaacaa ctggcgagg attgtctgt gcgacagtt ctggcaggg gagttgtcg ctaatggcg gctttagcg acaggacag ttagttaaa agtcactta aagtacaat cgttcgtat tcagagaag gattctcag gattgttgt aaaacttag
- >TC009557 RA
ctccaaaaa ataaaatgt tgtgttaag tgtgtaaaa tgcccccga acggcgatg tcgcgcgtg ttccgcttg tacggctcg ttctgcgcc acccacccg tgggaggtg atcgtggcc gtggtgacc ctcacgact tgcctgctc accgtcgac cagaagaag gagcccccg gcgcagccc tgccccgga tgcttcgag gagcagttc aaggcggtg gacgtcatc gtcatgacc gtgatccgg tgcttggcc atcctgtac agctcgcac caattccgc aacttgcaa aagctcggc tccaagtac atcctcggc ctcgccggc ctcttcatc gtgttctcc agcttcgtg ttcacttcc actgtcctg aatttcctc caaatcgat ctggtggat ctgaaggac gcgctgttc tttttcctg ttgttgatt gacctgtcc aaagcggcc actttggcc cagttcgcg ctgactgcc tccagtcag gaggagata agtagtaaa attgcaagg ggcatgtct gtcctgggg cccactatt acgctggat acggtcgtc gagactcta gtcattggg gtggggacc ctgtcgggg gtccaccgc ttggagatg ctttcgtat tttgcgtgt ctttccgtt ttagtcaat tatgttatt ttcatgacg ttctacccc gcgtgtttg tcgctaatg ttggagttg gcccgaatc tcgaatttc tacggggaa aagcaacca attgtctcg aaaatgctg ctcgaagag aacgaaaaa gcaaatcca gctgtacaa cgtgttaaa ctcataatg tcaatcgga ctcactgct gtgcacgtc cacagtcgt tggttcgtc aaagacgaa agcacagac agcgaaatt atcacaaac accgaccac caaaattgt gccgaagac tcagtttac ggttatttg atgaaacag ttcgctctg agtgccgac cacatcgta atcctaatc ctggtggtg accctgatc atcaaattc atatttttc gaaaacgac gaccacttg aaacaagct atagacgat gagccacct cacgtcctc aaacgccaa aaatgcatc accaaagcc gagtcaata aacgaggag aaggaagtt caaaccaac gacgaagaa atcgaaagt ttggacgag acaaacacc cgaaacctc gaagagtgt ctccagatt taccgctcg gacggagcg tccaaactt agcgataag gaagtgatc ctactcatt gacaccaaa cacttgccg gcctacaat ttggaaaaa gcggtcaag aatcaagaa cgcggggtt aaaatccgg cgccaactg atccaaagt ttggccaaa tgcgagtca gcgtttctc aacctcccg tataaacac tatgactat agtaaagtc atgggtgcg tgttgcgag aacgtgatt ggatacatt cccgtacca ttgggcgtg gctgggcct ttggtactt gatggtaaa caatactac atccccatg tcgaccact gagggttgc ctgattgcc agcgccaat cggggctgt cgtgcggtg gaacattgc ggtattaag agccgaatc gtttccgac ggaatgact cgagggcct gtcgttcgt tttaattcc ataacgaag gcgtccgaa gtcacgctc tggttggaa aaaccggag aatttcctc aaagtcaag gaaaatttc gattcgacg agtcgtttc gcgcgtttg tcgcggatt acaagccgg gttgctggt agatacctg tttttgcgg ttcgtggcc gagacgggt gatgccatg ggcatgaat atgctgtcg aaagggacg gaaaaagcg ctgtttttc gtccagagg ttcttcccc gagatggag attctgagt ttgagtggg aattactgc gcggataag aagccagcc gctgtgaat tgggtcgaa ggccggggg aagtccgtg gtttgtgaa gcgatagtt tcgggtgat gtcgtttcg tcagtatta aagtcgtcg acgcaggtc ttggtcgat ttgaacact tccaagaat atgatcggg tcggccatg gccgggagt attgggggg tttaatgcc cacgctgcc aacatcgtg accgcgatt ttcatcgca actggccaa gaccccgct cagaacata gccagtagt aactgtatt accataatg gagccgtgg ggcgacaat gggcaagat ttgtacgtt tcttgcacc atgccatcc atcgaaatc ggtaccatt ggcggtggt accatcctt ccagcgcag tctgcgtgt ttggatatg ttgggtgtg cgggggccc aacgaggag tccccagga gccaactcc aaacaactg gcgaggatt gtctgtgcg acagttctg gcaggggag ttgtcgcta atggcggct ttagcgaca ggacagtta gttaaaagt cacttaaag tacaatcgt tcgtattca gagaaggat tctcaggat tgttgtaaa acttagatt taataaata gtgaaaagt ata