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Gene information
General information
TC009546 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid47: 177937..181265
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009546
    iBeetle screen iB_01573
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009546 RA
      MTLNLTKEN RIYKKLFTL KPSQRDGLV LNIHHTDRN 
      AQKIRFLSC CFSKNGQTL ALADYEGNI FVIDFTSTK 
      FWNIPKFTN TCVMVKFSH FKEGEILVG AHCGAVYVV 
      EAQTGFVCG QLRGHQSPV NCMTFGSNF LCLTGSTSE 
      AILWDLNTN SKLQVLSLQ HSCALKYVC FIPVSSYIL 
      TCFNDDVIQ IWQFGTFEM KTEIPPLVW FNHEVKNIT 
      FTQDGKVMV IGCRSPHLA LFLLSNFKL FKFITLPEH 
      IQSLKHIDF IPQPNDAGS NKILAILSG RGTVYFYDS 
      HQNVLMSEL VANYEISKY DFSASYMAC VLSSGDVNI 
      YDVTQYVVV PLKLKAVRA ESARKKVKL KRCLEKMGI 
      VKEQIREIL EIEKLRMIL KECGEFPDS YRTKIWEHL 
      LALPNNREL YNGIINHRM IVSFEDLCR KYPLENKRN 
      LKNLKQLLD NLVTWCPFF LNVDYLPLF VFPFVKVFI 
      NKPLTCFEA VCTIILNWC QFWFEYFPL PPINILAMI 
      ENVLMVHDP ELLDFLTRK KVSSELYAW PILRTAFSE 
      VLTAPSWKI FWDHVLTNE PSFLLCGVV AYNILQRTS 
      ILSLKDDKQ FRCFYKTHH PIDVKKLLT KSYHILAKT 
      GEAVHPRQY LNMFKKINL GEYPNFCDY PKTMIEMSD 
      KEAEMKREM AGLKTDEVD LFRRRLISL ERLQNFEVE 
      EEENKRILE MERAIRNQL LEEQTRVQE YYERVANLR 
      EKFHQEDKI LASLRHQIA KEQQLKSRR KYLSSIMDD 
      VRFRKGEKG YALTKTEDE LLFHYAELL KNKYKIEKL 
      LNTDEVVTS TKLSVDQQQ EQLSNEINF VRSSEEPRK 
      HLKMLNLTT GLEAMDALI EKVESELEK QKSGSDSDA 
      DRRVVALQA ESKELALKI ANLIELLAN LRSEEVGG
    CDS sequence
    • >TC009546 RA
      atgactcta aacctaacc aaggaaaac cgcatttac 
      aagaaactt ttcactttg aaaccctcc caaagagac 
      ggcttagtg ctaaacatc caccacacc gacagaaac 
      gcgcagaag atccgcttt ctcagttgc tgtttcagc 
      aaaaatggt caaacactg gccttggcc gactacgag 
      ggcaacatc tttgtgatc gacttcacg agcaccaaa 
      ttctggaac atcccaaaa ttcacaaac acttgcgtt 
      atggtcaag ttttcgcac ttcaaagag ggcgaaatt 
      ttagtgggg gcacattgt ggcgctgtg tacgtggtt 
      gaggcccaa actggcttc gtctgcggc cagttgagg 
      ggccaccag agccctgtt aattgcatg acgtttggt 
      agtaacttt ttgtgcctc acggggtcc acaagtgag 
      gcgattttg tgggacttg aacaccaac agcaagctg 
      caggtgctc agtttgcag cacagttgc gccctgaaa 
      tatgtgtgc ttcattccc gtttcctcc tatattttg 
      acatgtttc aacgacgac gtgatccag atctggcag 
      tttggcacg tttgagatg aagactgaa ataccacct 
      ctggtgtgg ttcaaccac gaagtgaaa aacatcacg 
      ttcacacag gacggcaaa gtgatggtg attggttgc 
      cgctcacca cacctcgcc ctcttccta ctctcgaac 
      ttcaaacta tttaaattt ataacattg ccagaacac 
      atccagtca ctgaaacac attgatttc atcccccag 
      ccaaacgac gccggctcg aataaaatt ttagccatt 
      ttatcaggc cgtggcact gtttatttc tacgacagc 
      caccaaaat gttctaatg agcgaatta gttgcaaat 
      tacgaaatt tcaaaatac gatttttcg gcgagttat 
      atggcctgt gttttatcg tccggtgac gtcaatatc 
      tacgatgtg acccaatac gtggttgtg ccactcaaa 
      ctgaaggcc gttcgggca gaaagtgcg cgaaaaaaa 
      gtcaaattg aagcgatgt ttggagaaa atgggcata 
      gtcaaggag cagattagg gagattctc gagattgaa 
      aaattgaga atgatattg aaagagtgt ggcgagttt 
      cccgattca taccggacc aaaatttgg gaacacttg 
      ttggcactt cccaataat agggagctc tacaatggg 
      attatcaac caccgaatg atagtctca tttgaggat 
      ttgtgccgc aaatacccg cttgaaaac aagcgaaac 
      ctcaaaaac ctcaaacaa ctgttggat aatttggtc 
      acgtggtgt cccttcttc ctcaacgtg gattatttg 
      ccacttttt gtgttcccc tttgtcaag gtttttatt 
      aacaagccg ttgacgtgt tttgaggcc gtttgcacg 
      ataattttg aactggtgt caattctgg ttcgagtac 
      ttccctctc cctccaatc aacatactt gcgatgatt 
      gaaaacgtc ctaatggta cacgacccc gaacttttg 
      gacttccta accagaaaa aaagtctct tccgaattg 
      tacgcatgg ccgattttg cgaactgca ttttccgag 
      gttttgaca gcgccatcc tggaaaatt ttctgggac 
      catgtttta acaaacgaa ccttcgttt ctactgtgt 
      ggagtcgtg gcttacaat attttacaa cgaacctcc 
      attttgagt cttaaagac gataaacaa tttcggtgt 
      ttttacaaa acgcatcat cccatagat gttaaaaaa 
      ttgctaacg aaaagttac cacattttg gccaagacg 
      ggcgaggcc gtgcaccca cggcaatat ttgaacatg 
      ttcaagaaa attaatttg ggggaatac cccaatttt 
      tgtgattac cccaaaacc atgatcgag atgtcggat 
      aaagaggcc gagatgaag agagagatg gcggggttg 
      aaaacagac gaggttgat ttgttcaga cgccgattg 
      attagtttg gagcggttg caaaatttc gaagtggag 
      gaagaagag aataagcga attttggaa atggaacgc 
      gcgattcgt aatcaattg ttggaggag caaacccgt 
      gtccaggag tactacgaa agggtggcc aacctgcgc 
      gaaaaattc caccaagaa gacaaaatt ttggcctcc 
      ttaagacac caaatagcg aaggaacag caattgaaa 
      agccgccga aaataccta tcgtcgatt atggacgat 
      gtcaggttc cgaaaaggc gagaaaggt tacgccttg 
      accaagacc gaggacgaa ctcctcttc cactatgcc 
      gaacttttg aaaaacaag tacaaaatc gaaaagtta 
      ctaaacact gatgaagtt gtcacaagt acaaaacta 
      tcagttgat caacaacag gaacaatta tcgaacgaa 
      atcaatttt gtgcggagt agtgaagag ccccgcaaa 
      catttgaag atgttaaat ctgacaacg gggcttgaa 
      gccatggat gctctaatc gaaaaagtc gaaagtgag 
      ctggaaaaa caaaagagc gggagtgac agtgatgcc 
      gatcgacgc gttgtcgcc ctgcaagcg gaaagcaaa 
      gagttggcg ttgaaaatt gccaattta atcgagcta 
      cttgccaat ttaaggagt gaggaagtg ggcggatag
    mRNA sequence
    • >TC009546 RA
      cttgaactc gattttaca aaatattga cacagatta 
      gaggttatc ttgctttaa tctgtgacc atcgataaa 
      gacaaacca acccttgaa acaaccaca accaccatg 
      actctaaac ctaaccaag gaaaaccgc atttacaag 
      aaacttttc actttgaaa ccctcccaa agagacggc 
      ttagtgcta aacatccac cacaccgac agaaacgcg 
      cagaagatc cgctttctc agttgctgt ttcagcaaa 
      aatggtcaa acactggcc ttggccgac tacgagggc 
      aacatcttt gtgatcgac ttcacgagc accaaattc 
      tggaacatc ccaaaattc acaaacact tgcgttatg 
      gtcaagttt tcgcacttc aaagagggc gaaatttta 
      gtgggggca cattgtggc gctgtgtac gtggttgag 
      gcccaaact ggcttcgtc tgcggccag ttgaggggc 
      caccagagc cctgttaat tgcatgacg tttggtagt 
      aactttttg tgcctcacg gggtccaca agtgaggcg 
      attttgtgg gacttgaac accaacagc aagctgcag 
      gtgctcagt ttgcagcac agttgcgcc ctgaaatat 
      gtgtgcttc attcccgtt tcctcctat attttgaca 
      tgtttcaac gacgacgtg atccagatc tggcagttt 
      ggcacgttt gagatgaag actgaaata ccacctctg 
      gtgtggttc aaccacgaa gtgaaaaac atcacgttc 
      acacaggac ggcaaagtg atggtgatt ggttgccgc 
      tcaccacac ctcgccctc ttcctactc tcgaacttc 
      aaactattt aaatttata acattgcca gaacacatc 
      cagtcactg aaacacatt gatttcatc ccccagcca 
      aacgacgcc ggctcgaat aaaatttta gccatttta 
      tcaggccgt ggcactgtt tatttctac gacagccac 
      caaaatgtt ctaatgagc gaattagtt gcaaattac 
      gaaatttca aaatacgat ttttcggcg agttatatg 
      gcctgtgtt ttatcgtcc ggtgacgtc aatatctac 
      gatgtgacc caatacgtg gttgtgcca ctcaaactg 
      aaggccgtt cgggcagaa agtgcgcga aaaaaagtc 
      aaattgaag cgatgtttg gagaaaatg ggcatagtc 
      aaggagcag attagggag attctcgag attgaaaaa 
      ttgagaatg atattgaaa gagtgtggc gagtttccc 
      gattcatac cggaccaaa atttgggaa cacttgttg 
      gcacttccc aataatagg gagctctac aatgggatt 
      atcaaccac cgaatgata gtctcattt gaggatttg 
      tgccgcaaa tacccgctt gaaaacaag cgaaacctc 
      aaaaacctc aaacaactg ttggataat ttggtcacg 
      tggtgtccc ttcttcctc aacgtggat tatttgcca 
      ctttttgtg ttccccttt gtcaaggtt tttattaac 
      aagccgttg acgtgtttt gaggccgtt tgcacgata 
      attttgaac tggtgtcaa ttctggttc gagtacttc 
      cctctccct ccaatcaac atacttgcg atgattgaa 
      aacgtccta atggtacac gaccccgaa cttttggac 
      ttcctaacc agaaaaaaa gtctcttcc gaattgtac 
      gcatggccg attttgcga actgcattt tccgaggtt 
      ttgacagcg ccatcctgg aaaattttc tgggaccat 
      gttttaaca aacgaacct tcgtttcta ctgtgtgga 
      gtcgtggct tacaatatt ttacaacga acctccatt 
      ttgagtctt aaagacgat aaacaattt cggtgtttt 
      tacaaaacg catcatccc atagatgtt aaaaaattg 
      ctaacgaaa agttaccac attttggcc aagacgggc 
      gaggccgtg cacccacgg caatatttg aacatgttc 
      aagaaaatt aatttgggg gaatacccc aatttttgt 
      gattacccc aaaaccatg atcgagatg tcggataaa 
      gaggccgag atgaagaga gagatggcg gggttgaaa 
      acagacgag gttgatttg ttcagacgc cgattgatt 
      agtttggag cggttgcaa aatttcgaa gtggaggaa 
      gaagagaat aagcgaatt ttggaaatg gaacgcgcg 
      attcgtaat caattgttg gaggagcaa acccgtgtc 
      caggagtac tacgaaagg gtggccaac ctgcgcgaa 
      aaattccac caagaagac aaaattttg gcctcctta 
      agacaccaa atagcgaag gaacagcaa ttgaaaagc 
      cgccgaaaa tacctatcg tcgattatg gacgatgtc 
      aggttccga aaaggcgag aaaggttac gccttgacc 
      aagaccgag gacgaactc ctcttccac tatgccgaa 
      cttttgaaa aacaagtac aaaatcgaa aagttacta 
      aacactgat gaagttgtc acaagtaca aaactatca 
      gttgatcaa caacaggaa caattatcg aacgaaatc 
      aattttgtg cggagtagt gaagagccc cgcaaacat 
      ttgaagatg ttaaatctg acaacgggg cttgaagcc 
      atggatgct ctaatcgaa aaagtcgaa agtgagctg 
      gaaaaacaa aagagcggg agtgacagt gatgccgat 
      cgacgcgtt gtcgccctg caagcggaa agcaaagag 
      ttggcgttg aaaattgcc aatttaatc gagctactt 
      gccaattta aggagtgag gaagtgggc ggatagcac 
      aatcaattt tattataag ttgtatcgt ttctattta 
      tacgtttta tgatctcat atgcacctg tgatatgtt 
      tttaaacgg caataaatt tacaaaccg atg