| General information | |||
| TC009546 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 2259837..2263165 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC009546 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01573 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC009546 RA
MTLNLTKEN RIYKKLFTL KPSQRDGLV LNIHHTDRN AQKIRFLSC CFSKNGQTL ALADYEGNI FVIDFTSTK FWNIPKFTN TCVMVKFSH FKEGEILVG AHCGAVYVV EAQTGFVCG QLRGHQSPV NCMTFGSNF LCLTGSTSE AILWDLNTN SKLQVLSLQ HSCALKYVC FIPVSSYIL TCFNDDVIQ IWQFGTFEM KTEIPPLVW FNHEVKNIT FTQDGKVMV IGCRSPHLA LFLLSNFKL FKFITLPEH IQSLKHIDF IPQPNDAGS NKILAILSG RGTVYFYDS HQNVLMSEL VANYEISKY DFSASYMAC VLSSGDVNI YDVTQYVVV PLKLKAVRA ESARKKVKL KRCLEKMGI VKEQIREIL EIEKLRMIL KECGEFPDS YRTKIWEHL LALPNNREL YNGIINHRM IVSFEDLCR KYPLENKRN LKNLKQLLD NLVTWCPFF LNVDYLPLF VFPFVKVFI NKPLTCFEA VCTIILNWC QFWFEYFPL PPINILAMI ENVLMVHDP ELLDFLTRK KVSSELYAW PILRTAFSE VLTAPSWKI FWDHVLTNE PSFLLCGVV AYNILQRTS ILSLKDDKQ FRCFYKTHH PIDVKKLLT KSYHILAKT GEAVHPRQY LNMFKKINL GEYPNFCDY PKTMIEMSD KEAEMKREM AGLKTDEVD LFRRRLISL ERLQNFEVE EEENKRILE MERAIRNQL LEEQTRVQE YYERVANLR EKFHQEDKI LASLRHQIA KEQQLKSRR KYLSSIMDD VRFRKGEKG YALTKTEDE LLFHYAELL KNKYKIEKL LNTDEVVTS TKLSVDQQQ EQLSNEINF VRSSEEPRK HLKMLNLTT GLEAMDALI EKVESELEK QKSGSDSDA DRRVVALQA ESKELALKI ANLIELLAN LRSEEVGG
- >TC009546 RA
atgactcta aacctaacc aaggaaaac cgcatttac aagaaactt ttcactttg aaaccctcc caaagagac ggcttagtg ctaaacatc caccacacc gacagaaac gcgcagaag atccgcttt ctcagttgc tgtttcagc aaaaatggt caaacactg gccttggcc gactacgag ggcaacatc tttgtgatc gacttcacg agcaccaaa ttctggaac atcccaaaa ttcacaaac acttgcgtt atggtcaag ttttcgcac ttcaaagag ggcgaaatt ttagtgggg gcacattgt ggcgctgtg tacgtggtt gaggcccaa actggcttc gtctgcggc cagttgagg ggccaccag agccctgtt aattgcatg acgtttggt agtaacttt ttgtgcctc acggggtcc acaagtgag gcgattttg tgggacttg aacaccaac agcaagctg caggtgctc agtttgcag cacagttgc gccctgaaa tatgtgtgc ttcattccc gtttcctcc tatattttg acatgtttc aacgacgac gtgatccag atctggcag tttggcacg tttgagatg aagactgaa ataccacct ctggtgtgg ttcaaccac gaagtgaaa aacatcacg ttcacacag gacggcaaa gtgatggtg attggttgc cgctcacca cacctcgcc ctcttccta ctctcgaac ttcaaacta tttaaattt ataacattg ccagaacac atccagtca ctgaaacac attgatttc atcccccag ccaaacgac gccggctcg aataaaatt ttagccatt ttatcaggc cgtggcact gtttatttc tacgacagc caccaaaat gttctaatg agcgaatta gttgcaaat tacgaaatt tcaaaatac gatttttcg gcgagttat atggcctgt gttttatcg tccggtgac gtcaatatc tacgatgtg acccaatac gtggttgtg ccactcaaa ctgaaggcc gttcgggca gaaagtgcg cgaaaaaaa gtcaaattg aagcgatgt ttggagaaa atgggcata gtcaaggag cagattagg gagattctc gagattgaa aaattgaga atgatattg aaagagtgt ggcgagttt cccgattca taccggacc aaaatttgg gaacacttg ttggcactt cccaataat agggagctc tacaatggg attatcaac caccgaatg atagtctca tttgaggat ttgtgccgc aaatacccg cttgaaaac aagcgaaac ctcaaaaac ctcaaacaa ctgttggat aatttggtc acgtggtgt cccttcttc ctcaacgtg gattatttg ccacttttt gtgttcccc tttgtcaag gtttttatt aacaagccg ttgacgtgt tttgaggcc gtttgcacg ataattttg aactggtgt caattctgg ttcgagtac ttccctctc cctccaatc aacatactt gcgatgatt gaaaacgtc ctaatggta cacgacccc gaacttttg gacttccta accagaaaa aaagtctct tccgaattg tacgcatgg ccgattttg cgaactgca ttttccgag gttttgaca gcgccatcc tggaaaatt ttctgggac catgtttta acaaacgaa ccttcgttt ctactgtgt ggagtcgtg gcttacaat attttacaa cgaacctcc attttgagt cttaaagac gataaacaa tttcggtgt ttttacaaa acgcatcat cccatagat gttaaaaaa ttgctaacg aaaagttac cacattttg gccaagacg ggcgaggcc gtgcaccca cggcaatat ttgaacatg ttcaagaaa attaatttg ggggaatac cccaatttt tgtgattac cccaaaacc atgatcgag atgtcggat aaagaggcc gagatgaag agagagatg gcggggttg aaaacagac gaggttgat ttgttcaga cgccgattg attagtttg gagcggttg caaaatttc gaagtggag gaagaagag aataagcga attttggaa atggaacgc gcgattcgt aatcaattg ttggaggag caaacccgt gtccaggag tactacgaa agggtggcc aacctgcgc gaaaaattc caccaagaa gacaaaatt ttggcctcc ttaagacac caaatagcg aaggaacag caattgaaa agccgccga aaataccta tcgtcgatt atggacgat gtcaggttc cgaaaaggc gagaaaggt tacgccttg accaagacc gaggacgaa ctcctcttc cactatgcc gaacttttg aaaaacaag tacaaaatc gaaaagtta ctaaacact gatgaagtt gtcacaagt acaaaacta tcagttgat caacaacag gaacaatta tcgaacgaa atcaatttt gtgcggagt agtgaagag ccccgcaaa catttgaag atgttaaat ctgacaacg gggcttgaa gccatggat gctctaatc gaaaaagtc gaaagtgag ctggaaaaa caaaagagc gggagtgac agtgatgcc gatcgacgc gttgtcgcc ctgcaagcg gaaagcaaa gagttggcg ttgaaaatt gccaattta atcgagcta cttgccaat ttaaggagt gaggaagtg ggcggatag
- >TC009546 RA
cttgaactc gattttaca aaatattga cacagatta gaggttatc ttgctttaa tctgtgacc atcgataaa gacaaacca acccttgaa acaaccaca accaccatg actctaaac ctaaccaag gaaaaccgc atttacaag aaacttttc actttgaaa ccctcccaa agagacggc ttagtgcta aacatccac cacaccgac agaaacgcg cagaagatc cgctttctc agttgctgt ttcagcaaa aatggtcaa acactggcc ttggccgac tacgagggc aacatcttt gtgatcgac ttcacgagc accaaattc tggaacatc ccaaaattc acaaacact tgcgttatg gtcaagttt tcgcacttc aaagagggc gaaatttta gtgggggca cattgtggc gctgtgtac gtggttgag gcccaaact ggcttcgtc tgcggccag ttgaggggc caccagagc cctgttaat tgcatgacg tttggtagt aactttttg tgcctcacg gggtccaca agtgaggcg attttgtgg gacttgaac accaacagc aagctgcag gtgctcagt ttgcagcac agttgcgcc ctgaaatat gtgtgcttc attcccgtt tcctcctat attttgaca tgtttcaac gacgacgtg atccagatc tggcagttt ggcacgttt gagatgaag actgaaata ccacctctg gtgtggttc aaccacgaa gtgaaaaac atcacgttc acacaggac ggcaaagtg atggtgatt ggttgccgc tcaccacac ctcgccctc ttcctactc tcgaacttc aaactattt aaatttata acattgcca gaacacatc cagtcactg aaacacatt gatttcatc ccccagcca aacgacgcc ggctcgaat aaaatttta gccatttta tcaggccgt ggcactgtt tatttctac gacagccac caaaatgtt ctaatgagc gaattagtt gcaaattac gaaatttca aaatacgat ttttcggcg agttatatg gcctgtgtt ttatcgtcc ggtgacgtc aatatctac gatgtgacc caatacgtg gttgtgcca ctcaaactg aaggccgtt cgggcagaa agtgcgcga aaaaaagtc aaattgaag cgatgtttg gagaaaatg ggcatagtc aaggagcag attagggag attctcgag attgaaaaa ttgagaatg atattgaaa gagtgtggc gagtttccc gattcatac cggaccaaa atttgggaa cacttgttg gcacttccc aataatagg gagctctac aatgggatt atcaaccac cgaatgata gtctcattt gaggatttg tgccgcaaa tacccgctt gaaaacaag cgaaacctc aaaaacctc aaacaactg ttggataat ttggtcacg tggtgtccc ttcttcctc aacgtggat tatttgcca ctttttgtg ttccccttt gtcaaggtt tttattaac aagccgttg acgtgtttt gaggccgtt tgcacgata attttgaac tggtgtcaa ttctggttc gagtacttc cctctccct ccaatcaac atacttgcg atgattgaa aacgtccta atggtacac gaccccgaa cttttggac ttcctaacc agaaaaaaa gtctcttcc gaattgtac gcatggccg attttgcga actgcattt tccgaggtt ttgacagcg ccatcctgg aaaattttc tgggaccat gttttaaca aacgaacct tcgtttcta ctgtgtgga gtcgtggct tacaatatt ttacaacga acctccatt ttgagtctt aaagacgat aaacaattt cggtgtttt tacaaaacg catcatccc atagatgtt aaaaaattg ctaacgaaa agttaccac attttggcc aagacgggc gaggccgtg cacccacgg caatatttg aacatgttc aagaaaatt aatttgggg gaatacccc aatttttgt gattacccc aaaaccatg atcgagatg tcggataaa gaggccgag atgaagaga gagatggcg gggttgaaa acagacgag gttgatttg ttcagacgc cgattgatt agtttggag cggttgcaa aatttcgaa gtggaggaa gaagagaat aagcgaatt ttggaaatg gaacgcgcg attcgtaat caattgttg gaggagcaa acccgtgtc caggagtac tacgaaagg gtggccaac ctgcgcgaa aaattccac caagaagac aaaattttg gcctcctta agacaccaa atagcgaag gaacagcaa ttgaaaagc cgccgaaaa tacctatcg tcgattatg gacgatgtc aggttccga aaaggcgag aaaggttac gccttgacc aagaccgag gacgaactc ctcttccac tatgccgaa cttttgaaa aacaagtac aaaatcgaa aagttacta aacactgat gaagttgtc acaagtaca aaactatca gttgatcaa caacaggaa caattatcg aacgaaatc aattttgtg cggagtagt gaagagccc cgcaaacat ttgaagatg ttaaatctg acaacgggg cttgaagcc atggatgct ctaatcgaa aaagtcgaa agtgagctg gaaaaacaa aagagcggg agtgacagt gatgccgat cgacgcgtt gtcgccctg caagcggaa agcaaagag ttggcgttg aaaattgcc aatttaatc gagctactt gccaattta aggagtgag gaagtgggc ggatagcac aatcaattt tattataag ttgtatcgt ttctattta tacgtttta tgatctcat atgcacctg tgatatgtt tttaaacgg caataaatt tacaaaccg atg
