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Gene information
General information
TC009546 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 2259837..2263165
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009546
    iBeetle screen iB_01573

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009546 RA
      MTLNLTKEN RIYKKLFTL KPSQRDGLV LNIHHTDRN 
AQKIRFLSC CFSKNGQTL ALADYEGNI FVIDFTSTK 
FWNIPKFTN TCVMVKFSH FKEGEILVG AHCGAVYVV 
EAQTGFVCG QLRGHQSPV NCMTFGSNF LCLTGSTSE 
AILWDLNTN SKLQVLSLQ HSCALKYVC FIPVSSYIL 
TCFNDDVIQ IWQFGTFEM KTEIPPLVW FNHEVKNIT 
FTQDGKVMV IGCRSPHLA LFLLSNFKL FKFITLPEH 
IQSLKHIDF IPQPNDAGS NKILAILSG RGTVYFYDS 
HQNVLMSEL VANYEISKY DFSASYMAC VLSSGDVNI 
YDVTQYVVV PLKLKAVRA ESARKKVKL KRCLEKMGI 
VKEQIREIL EIEKLRMIL KECGEFPDS YRTKIWEHL 
LALPNNREL YNGIINHRM IVSFEDLCR KYPLENKRN 
LKNLKQLLD NLVTWCPFF LNVDYLPLF VFPFVKVFI 
NKPLTCFEA VCTIILNWC QFWFEYFPL PPINILAMI 
ENVLMVHDP ELLDFLTRK KVSSELYAW PILRTAFSE 
VLTAPSWKI FWDHVLTNE PSFLLCGVV AYNILQRTS 
ILSLKDDKQ FRCFYKTHH PIDVKKLLT KSYHILAKT 
GEAVHPRQY LNMFKKINL GEYPNFCDY PKTMIEMSD 
KEAEMKREM AGLKTDEVD LFRRRLISL ERLQNFEVE 
EEENKRILE MERAIRNQL LEEQTRVQE YYERVANLR 
EKFHQEDKI LASLRHQIA KEQQLKSRR KYLSSIMDD 
VRFRKGEKG YALTKTEDE LLFHYAELL KNKYKIEKL 
LNTDEVVTS TKLSVDQQQ EQLSNEINF VRSSEEPRK 
HLKMLNLTT GLEAMDALI EKVESELEK QKSGSDSDA 
DRRVVALQA ESKELALKI ANLIELLAN LRSEEVGG
    CDS sequence
    • >TC009546 RA
      atgactcta aacctaacc aaggaaaac cgcatttac 
aagaaactt ttcactttg aaaccctcc caaagagac 
ggcttagtg ctaaacatc caccacacc gacagaaac 
gcgcagaag atccgcttt ctcagttgc tgtttcagc 
aaaaatggt caaacactg gccttggcc gactacgag 
ggcaacatc tttgtgatc gacttcacg agcaccaaa 
ttctggaac atcccaaaa ttcacaaac acttgcgtt 
atggtcaag ttttcgcac ttcaaagag ggcgaaatt 
ttagtgggg gcacattgt ggcgctgtg tacgtggtt 
gaggcccaa actggcttc gtctgcggc cagttgagg 
ggccaccag agccctgtt aattgcatg acgtttggt 
agtaacttt ttgtgcctc acggggtcc acaagtgag 
gcgattttg tgggacttg aacaccaac agcaagctg 
caggtgctc agtttgcag cacagttgc gccctgaaa 
tatgtgtgc ttcattccc gtttcctcc tatattttg 
acatgtttc aacgacgac gtgatccag atctggcag 
tttggcacg tttgagatg aagactgaa ataccacct 
ctggtgtgg ttcaaccac gaagtgaaa aacatcacg 
ttcacacag gacggcaaa gtgatggtg attggttgc 
cgctcacca cacctcgcc ctcttccta ctctcgaac 
ttcaaacta tttaaattt ataacattg ccagaacac 
atccagtca ctgaaacac attgatttc atcccccag 
ccaaacgac gccggctcg aataaaatt ttagccatt 
ttatcaggc cgtggcact gtttatttc tacgacagc 
caccaaaat gttctaatg agcgaatta gttgcaaat 
tacgaaatt tcaaaatac gatttttcg gcgagttat 
atggcctgt gttttatcg tccggtgac gtcaatatc 
tacgatgtg acccaatac gtggttgtg ccactcaaa 
ctgaaggcc gttcgggca gaaagtgcg cgaaaaaaa 
gtcaaattg aagcgatgt ttggagaaa atgggcata 
gtcaaggag cagattagg gagattctc gagattgaa 
aaattgaga atgatattg aaagagtgt ggcgagttt 
cccgattca taccggacc aaaatttgg gaacacttg 
ttggcactt cccaataat agggagctc tacaatggg 
attatcaac caccgaatg atagtctca tttgaggat 
ttgtgccgc aaatacccg cttgaaaac aagcgaaac 
ctcaaaaac ctcaaacaa ctgttggat aatttggtc 
acgtggtgt cccttcttc ctcaacgtg gattatttg 
ccacttttt gtgttcccc tttgtcaag gtttttatt 
aacaagccg ttgacgtgt tttgaggcc gtttgcacg 
ataattttg aactggtgt caattctgg ttcgagtac 
ttccctctc cctccaatc aacatactt gcgatgatt 
gaaaacgtc ctaatggta cacgacccc gaacttttg 
gacttccta accagaaaa aaagtctct tccgaattg 
tacgcatgg ccgattttg cgaactgca ttttccgag 
gttttgaca gcgccatcc tggaaaatt ttctgggac 
catgtttta acaaacgaa ccttcgttt ctactgtgt 
ggagtcgtg gcttacaat attttacaa cgaacctcc 
attttgagt cttaaagac gataaacaa tttcggtgt 
ttttacaaa acgcatcat cccatagat gttaaaaaa 
ttgctaacg aaaagttac cacattttg gccaagacg 
ggcgaggcc gtgcaccca cggcaatat ttgaacatg 
ttcaagaaa attaatttg ggggaatac cccaatttt 
tgtgattac cccaaaacc atgatcgag atgtcggat 
aaagaggcc gagatgaag agagagatg gcggggttg 
aaaacagac gaggttgat ttgttcaga cgccgattg 
attagtttg gagcggttg caaaatttc gaagtggag 
gaagaagag aataagcga attttggaa atggaacgc 
gcgattcgt aatcaattg ttggaggag caaacccgt 
gtccaggag tactacgaa agggtggcc aacctgcgc 
gaaaaattc caccaagaa gacaaaatt ttggcctcc 
ttaagacac caaatagcg aaggaacag caattgaaa 
agccgccga aaataccta tcgtcgatt atggacgat 
gtcaggttc cgaaaaggc gagaaaggt tacgccttg 
accaagacc gaggacgaa ctcctcttc cactatgcc 
gaacttttg aaaaacaag tacaaaatc gaaaagtta 
ctaaacact gatgaagtt gtcacaagt acaaaacta 
tcagttgat caacaacag gaacaatta tcgaacgaa 
atcaatttt gtgcggagt agtgaagag ccccgcaaa 
catttgaag atgttaaat ctgacaacg gggcttgaa 
gccatggat gctctaatc gaaaaagtc gaaagtgag 
ctggaaaaa caaaagagc gggagtgac agtgatgcc 
gatcgacgc gttgtcgcc ctgcaagcg gaaagcaaa 
gagttggcg ttgaaaatt gccaattta atcgagcta 
cttgccaat ttaaggagt gaggaagtg ggcggatag
    mRNA sequence
    • >TC009546 RA
      cttgaactc gattttaca aaatattga cacagatta 
gaggttatc ttgctttaa tctgtgacc atcgataaa 
gacaaacca acccttgaa acaaccaca accaccatg 
actctaaac ctaaccaag gaaaaccgc atttacaag 
aaacttttc actttgaaa ccctcccaa agagacggc 
ttagtgcta aacatccac cacaccgac agaaacgcg 
cagaagatc cgctttctc agttgctgt ttcagcaaa 
aatggtcaa acactggcc ttggccgac tacgagggc 
aacatcttt gtgatcgac ttcacgagc accaaattc 
tggaacatc ccaaaattc acaaacact tgcgttatg 
gtcaagttt tcgcacttc aaagagggc gaaatttta 
gtgggggca cattgtggc gctgtgtac gtggttgag 
gcccaaact ggcttcgtc tgcggccag ttgaggggc 
caccagagc cctgttaat tgcatgacg tttggtagt 
aactttttg tgcctcacg gggtccaca agtgaggcg 
attttgtgg gacttgaac accaacagc aagctgcag 
gtgctcagt ttgcagcac agttgcgcc ctgaaatat 
gtgtgcttc attcccgtt tcctcctat attttgaca 
tgtttcaac gacgacgtg atccagatc tggcagttt 
ggcacgttt gagatgaag actgaaata ccacctctg 
gtgtggttc aaccacgaa gtgaaaaac atcacgttc 
acacaggac ggcaaagtg atggtgatt ggttgccgc 
tcaccacac ctcgccctc ttcctactc tcgaacttc 
aaactattt aaatttata acattgcca gaacacatc 
cagtcactg aaacacatt gatttcatc ccccagcca 
aacgacgcc ggctcgaat aaaatttta gccatttta 
tcaggccgt ggcactgtt tatttctac gacagccac 
caaaatgtt ctaatgagc gaattagtt gcaaattac 
gaaatttca aaatacgat ttttcggcg agttatatg 
gcctgtgtt ttatcgtcc ggtgacgtc aatatctac 
gatgtgacc caatacgtg gttgtgcca ctcaaactg 
aaggccgtt cgggcagaa agtgcgcga aaaaaagtc 
aaattgaag cgatgtttg gagaaaatg ggcatagtc 
aaggagcag attagggag attctcgag attgaaaaa 
ttgagaatg atattgaaa gagtgtggc gagtttccc 
gattcatac cggaccaaa atttgggaa cacttgttg 
gcacttccc aataatagg gagctctac aatgggatt 
atcaaccac cgaatgata gtctcattt gaggatttg 
tgccgcaaa tacccgctt gaaaacaag cgaaacctc 
aaaaacctc aaacaactg ttggataat ttggtcacg 
tggtgtccc ttcttcctc aacgtggat tatttgcca 
ctttttgtg ttccccttt gtcaaggtt tttattaac 
aagccgttg acgtgtttt gaggccgtt tgcacgata 
attttgaac tggtgtcaa ttctggttc gagtacttc 
cctctccct ccaatcaac atacttgcg atgattgaa 
aacgtccta atggtacac gaccccgaa cttttggac 
ttcctaacc agaaaaaaa gtctcttcc gaattgtac 
gcatggccg attttgcga actgcattt tccgaggtt 
ttgacagcg ccatcctgg aaaattttc tgggaccat 
gttttaaca aacgaacct tcgtttcta ctgtgtgga 
gtcgtggct tacaatatt ttacaacga acctccatt 
ttgagtctt aaagacgat aaacaattt cggtgtttt 
tacaaaacg catcatccc atagatgtt aaaaaattg 
ctaacgaaa agttaccac attttggcc aagacgggc 
gaggccgtg cacccacgg caatatttg aacatgttc 
aagaaaatt aatttgggg gaatacccc aatttttgt 
gattacccc aaaaccatg atcgagatg tcggataaa 
gaggccgag atgaagaga gagatggcg gggttgaaa 
acagacgag gttgatttg ttcagacgc cgattgatt 
agtttggag cggttgcaa aatttcgaa gtggaggaa 
gaagagaat aagcgaatt ttggaaatg gaacgcgcg 
attcgtaat caattgttg gaggagcaa acccgtgtc 
caggagtac tacgaaagg gtggccaac ctgcgcgaa 
aaattccac caagaagac aaaattttg gcctcctta 
agacaccaa atagcgaag gaacagcaa ttgaaaagc 
cgccgaaaa tacctatcg tcgattatg gacgatgtc 
aggttccga aaaggcgag aaaggttac gccttgacc 
aagaccgag gacgaactc ctcttccac tatgccgaa 
cttttgaaa aacaagtac aaaatcgaa aagttacta 
aacactgat gaagttgtc acaagtaca aaactatca 
gttgatcaa caacaggaa caattatcg aacgaaatc 
aattttgtg cggagtagt gaagagccc cgcaaacat 
ttgaagatg ttaaatctg acaacgggg cttgaagcc 
atggatgct ctaatcgaa aaagtcgaa agtgagctg 
gaaaaacaa aagagcggg agtgacagt gatgccgat 
cgacgcgtt gtcgccctg caagcggaa agcaaagag 
ttggcgttg aaaattgcc aatttaatc gagctactt 
gccaattta aggagtgag gaagtgggc ggatagcac 
aatcaattt tattataag ttgtatcgt ttctattta 
tacgtttta tgatctcat atgcacctg tgatatgtt 
tttaaacgg caataaatt tacaaaccg atg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY