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Gene information
General information
TC009538 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid47: 120597..124301
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009538
    iBeetle screen iB_01571
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009538 RA
      MEVDLLRPG TVPIAPDTV LWFEFLLHH DLLLSHLQK 
      PVPDPGPTE LIAKFSDAI ADTLRNRLE TENGDVMLI 
      ESMHENVKQ PAKNIALKI LSLKVAAFI KWDLAQIRS 
      LPFKTQIHL LQVLLYFTN DKTTAEIPN IDLKPDESV 
      PSPYLFALV VYHRWLLNV TMHRITSNW QMRLGNTEI 
      SPAEENIIC SHENVRKTV EFLKSALSW DEVPSMLTF 
      DCFKLPTET NDLEFDWSS AQSITKEEF SAQLNYDLG 
      TFFFYKEDY NMARDHFAK CLSSYKAMK DNNGFVKFN 
      VSVLEVYVR ACSGDGSKG SLLEQLNTS VVNQFMGIT 
      SILQQDNIL REIPLSHRI TLELDIQGA LSSGTFTVA 
      RDLLNKIRA LNVVRCVLD QKPTYHCSL KSGDILIWA 
      VEATWKSLN DTDKHLVKK FILDLVLKN EIPDLLSCV 
      QANETLAKL FDKSDLSFI KTTESHDEI PESLYKTSW 
      TLYDCFKKR KPKLEIKQL EQDLIATYD HKEIRELLK 
      KISMNQLAT NVWNINAQW DLPIPIQSV IKSLPKGFL 
      HDFSFVMMA KAREQMLNK VWNSSLELL LILDKELGS 
      GNGNIGKLS RLISWEILL VQITQLLEE WPKSTIDKV 
      ALADACEAC LQTNNESVL PRTEITEQC VLCLLNLGR 
      WEFLINCDK RWPSFEITS AIALACQEL IKQKGNKKL 
      SKNLWEIVL PIFGHAPQQ SKRGNSGNS VFHDAQAQI 
      TNLRNNVMA IFGKLRDST VLAVVISML ARLHNILKD 
      ETSLEVQVD YVMLWPAVI SNANSYNMK IAHDVLSQM 
      VTQALQYYP TSIPWLRLL GDINFANGH YNVSLKYYL 
      KSLLIYNDY FNIPVRHDD HVFRRMIKC CIALGCNTQ 
      AAVLCQFLE EPDYVLAFR ILGDQKYCN DAVDAYYHC 
      IWDTNILEY LIHTHNKRG EYQRRKCAT QVIGSLEVN 
      SNNNEEIQR EASNLRKST FLRALCKQY VF
    CDS sequence
    • >TC009538 RA
      atggaggtt gatttgttg cgtccaggg acagtcccg 
      atagccccc gacactgtc ctctggttc gagtttttg 
      ctccaccat gacctccta ctgagccac ctccaaaaa 
      ccagtccca gacccggga cccacggaa ttaattgcc 
      aaattctcc gatgccatc gctgatacg ctacgaaat 
      cgactggag accgaaaac ggcgacgtt atgctcata 
      gaatcaatg cacgaaaat gtgaaacaa ccggcgaaa 
      aatatcgcc ctgaaaata ctatcgttg aaagttgca 
      gcgtttatt aaatgggac ctggcacaa atcaggtcg 
      ttgcccttc aagacccaa atccattta ttgcaagtc 
      ttactatat tttactaat gataaaaca acggccgaa 
      ataccaaat attgacctc aaaccggac gagtctgtc 
      ccatcgccc tatctcttc gcccttgtg gtgtaccat 
      aggtggtta cttaatgtc accatgcac aggattacc 
      tcaaactgg caaatgcgg ctaggcaac acggagata 
      tcaccagcg gaagagaac ataatttgc tcgcacgag 
      aatgtgcgg aaaacggtt gaattcctc aaaagtgcg 
      ctcagctgg gacgaagtc cccagtatg ctaactttc 
      gattgcttc aaactgcca actgagacc aacgatctt 
      gaattcgat tggtcctca gctcagagt atcacaaaa 
      gaggagttt agcgcccaa ttaaactac gacttgggg 
      acgtttttc ttctacaag gaggactac aacatggct 
      cgtgaccat tttgctaaa tgcttgagt agttacaaa 
      gccatgaaa gataataac ggttttgtt aaatttaat 
      gtaagtgtg ctggaggtg tatgtgagg gcatgtagc 
      ggcgatggt tcaaaaggc agtcttttg gaacagttg 
      aatacgtcc gtggtcaac cagttcatg gggattact 
      tcaatttta caacaggat aatattttg cgtgaaatc 
      cccctctca catcgaatt acgctcgag ttggacatt 
      cagggggct ttatcgagt ggcacgttc acagttgct 
      cgcgattta ttaaataaa atcagggcc ttgaacgtg 
      gtcaggtgt gttttggac caaaaaccc acctatcat 
      tgctcactt aaaagtggc gatatttta atttgggct 
      gtcgaagca acgtggaag tcactcaac gacactgat 
      aaacatttg gttaagaaa ttcatccta gacttggtc 
      ctaaaaaac gaaatccct gatcttttg tcgtgtgtt 
      caagccaat gaaacactt gcaaaatta tttgataag 
      agcgacctc agttttatt aaaaccacc gaaagtcac 
      gacgaaata cccgaatct ttgtacaag acaagttgg 
      acgctgtac gattgtttt aagaaacgt aaacccaaa 
      ttggaaatt aaacaactg gagcaagac ttaattgca 
      acttacgac cataaggaa attcgcgag ttgttgaaa 
      aaaatcagc atgaaccaa cttgcaact aacgtctgg 
      aatatcaac gcacagtgg gatttaccg attcccatc 
      cagtcagtg attaagtca ttgccgaaa ggtttcttg 
      catgatttc agtttcgtt atgatggcc aaagccagg 
      gaacaaatg ttgaataaa gtgtggaac tcatcgctt 
      gagttgtta ctaattttg gacaaagag ctcggttct 
      ggtaacggt aacattggc aaattatca cgtctgata 
      agctgggag atactacta gtgcaaata acccaactg 
      ttggaggag tggccaaaa agcaccatt gacaaggtg 
      gcccttgcc gatgcgtgt gaggcttgc ctccaaacc 
      aacaacgaa tcagtcttg ccacgcacc gaaataaca 
      gaacagtgc gtcctttgt ttgctaaat ctcggccgt 
      tgggagttt ttaattaat tgcgataaa aggtggccc 
      tcgtttgaa atcacctca gcgattgcg ttagcctgc 
      caggaattg atcaagcag aaagggaac aaaaaatta 
      tccaagaat ttgtgggag attgtcttg ccaattttt 
      ggccatgcc ccgcaacaa tcaaaacgg gggaattcc 
      ggaaattcg gtgtttcac gacgctcaa gcacagata 
      actaatttg cgaaataac gtaatggcg atttttggt 
      aaactgaga gactcgact gttttggct gtggttatt 
      tcgatgttg gcacgactg cataatatt ctgaaggat 
      gagaccagc ttggaggtg caggtggat tatgtgatg 
      ctttggccg gccgtgatc agcaacgcc aattcatac 
      aacatgaaa atagcccac gacgttctt tcccaaatg 
      gtaactcaa gccctccag tactatcca acaagcatt 
      ccttggttg aggctattg ggggacata aatttcgca 
      aacggtcat tacaacgta tctttgaaa tattatctc 
      aaatccctg ctaatttac aacgactat ttcaacatt 
      ccggtgcgt cacgacgac cacgtcttc cgccgcatg 
      attaagtgt tgcatagcc ttggggtgc aacacccag 
      gcggccgtt ctgtgccaa ttcctggaa gagcccgac 
      tatgtttta gcgttccgt atattgggg gaccagaaa 
      tattgcaat gacgccgtc gatgcgtat taccattgc 
      atttgggac accaatatt ctggaatat ctgatacac 
      acccacaac aaacggggg gagtaccag agacgcaaa 
      tgcgcaacg caggtgatt ggctcgttg gaggtgaat 
      tcgaacaac aacgaggag attcaaagg gaagcgagt 
      aatttacgg aaaagcacg tttctgagg gcgctatgc 
      aaacaatac gtgttttaa
    mRNA sequence
    • >TC009538 RA
      acctaaagt taaaatacc atggaggtt gatttgttg 
      cgtccaggg acagtcccg atagccccc gacactgtc 
      ctctggttc gagtttttg ctccaccat gacctccta 
      ctgagccac ctccaaaaa ccagtccca gacccggga 
      cccacggaa ttaattgcc aaattctcc gatgccatc 
      gctgatacg ctacgaaat cgactggag accgaaaac 
      ggcgacgtt atgctcata gaatcaatg cacgaaaat 
      gtgaaacaa ccggcgaaa aatatcgcc ctgaaaata 
      ctatcgttg aaagttgca gcgtttatt aaatgggac 
      ctggcacaa atcaggtcg ttgcccttc aagacccaa 
      atccattta ttgcaagtc ttactatat tttactaat 
      gataaaaca acggccgaa ataccaaat attgacctc 
      aaaccggac gagtctgtc ccatcgccc tatctcttc 
      gcccttgtg gtgtaccat aggtggtta cttaatgtc 
      accatgcac aggattacc tcaaactgg caaatgcgg 
      ctaggcaac acggagata tcaccagcg gaagagaac 
      ataatttgc tcgcacgag aatgtgcgg aaaacggtt 
      gaattcctc aaaagtgcg ctcagctgg gacgaagtc 
      cccagtatg ctaactttc gattgcttc aaactgcca 
      actgagacc aacgatctt gaattcgat tggtcctca 
      gctcagagt atcacaaaa gaggagttt agcgcccaa 
      ttaaactac gacttgggg acgtttttc ttctacaag 
      gaggactac aacatggct cgtgaccat tttgctaaa 
      tgcttgagt agttacaaa gccatgaaa gataataac 
      ggttttgtt aaatttaat gtaagtgtg ctggaggtg 
      tatgtgagg gcatgtagc ggcgatggt tcaaaaggc 
      agtcttttg gaacagttg aatacgtcc gtggtcaac 
      cagttcatg gggattact tcaatttta caacaggat 
      aatattttg cgtgaaatc cccctctca catcgaatt 
      acgctcgag ttggacatt cagggggct ttatcgagt 
      ggcacgttc acagttgct cgcgattta ttaaataaa 
      atcagggcc ttgaacgtg gtcaggtgt gttttggac 
      caaaaaccc acctatcat tgctcactt aaaagtggc 
      gatatttta atttgggct gtcgaagca acgtggaag 
      tcactcaac gacactgat aaacatttg gttaagaaa 
      ttcatccta gacttggtc ctaaaaaac gaaatccct 
      gatcttttg tcgtgtgtt caagccaat gaaacactt 
      gcaaaatta tttgataag agcgacctc agttttatt 
      aaaaccacc gaaagtcac gacgaaata cccgaatct 
      ttgtacaag acaagttgg acgctgtac gattgtttt 
      aagaaacgt aaacccaaa ttggaaatt aaacaactg 
      gagcaagac ttaattgca acttacgac cataaggaa 
      attcgcgag ttgttgaaa aaaatcagc atgaaccaa 
      cttgcaact aacgtctgg aatatcaac gcacagtgg 
      gatttaccg attcccatc cagtcagtg attaagtca 
      ttgccgaaa ggtttcttg catgatttc agtttcgtt 
      atgatggcc aaagccagg gaacaaatg ttgaataaa 
      gtgtggaac tcatcgctt gagttgtta ctaattttg 
      gacaaagag ctcggttct ggtaacggt aacattggc 
      aaattatca cgtctgata agctgggag atactacta 
      gtgcaaata acccaactg ttggaggag tggccaaaa 
      agcaccatt gacaaggtg gcccttgcc gatgcgtgt 
      gaggcttgc ctccaaacc aacaacgaa tcagtcttg 
      ccacgcacc gaaataaca gaacagtgc gtcctttgt 
      ttgctaaat ctcggccgt tgggagttt ttaattaat 
      tgcgataaa aggtggccc tcgtttgaa atcacctca 
      gcgattgcg ttagcctgc caggaattg atcaagcag 
      aaagggaac aaaaaatta tccaagaat ttgtgggag 
      attgtcttg ccaattttt ggccatgcc ccgcaacaa 
      tcaaaacgg gggaattcc ggaaattcg gtgtttcac 
      gacgctcaa gcacagata actaatttg cgaaataac 
      gtaatggcg atttttggt aaactgaga gactcgact 
      gttttggct gtggttatt tcgatgttg gcacgactg 
      cataatatt ctgaaggat gagaccagc ttggaggtg 
      caggtggat tatgtgatg ctttggccg gccgtgatc 
      agcaacgcc aattcatac aacatgaaa atagcccac 
      gacgttctt tcccaaatg gtaactcaa gccctccag 
      tactatcca acaagcatt ccttggttg aggctattg 
      ggggacata aatttcgca aacggtcat tacaacgta 
      tctttgaaa tattatctc aaatccctg ctaatttac 
      aacgactat ttcaacatt ccggtgcgt cacgacgac 
      cacgtcttc cgccgcatg attaagtgt tgcatagcc 
      ttggggtgc aacacccag gcggccgtt ctgtgccaa 
      ttcctggaa gagcccgac tatgtttta gcgttccgt 
      atattgggg gaccagaaa tattgcaat gacgccgtc 
      gatgcgtat taccattgc atttgggac accaatatt 
      ctggaatat ctgatacac acccacaac aaacggggg 
      gagtaccag agacgcaaa tgcgcaacg caggtgatt 
      ggctcgttg gaggtgaat tcgaacaac aacgaggag 
      attcaaagg gaagcgagt aatttacgg aaaagcacg 
      tttctgagg gcgctatgc aaacaatac gtgttttaa 
      cagttctat tatgtaata atagctaca attgtaaaa 
      a