Status
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Gene information
General information
TC009506 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 158723..171510
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009506
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01567

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009506 RA
      MRGMFSRFF IAAWILFGT VLNLQVTET NLRGLLTNL 
      EWLSLIGSS NSGSKFSIV YPHLTTSDD ALVKKLEIE 
      TPEKKFMFE LVPNPDLLS KNFLVFETF ANGSYPMHF 
      IDDDARCHY RGDKSALCL CNGIKGLLY FDNDSLYII 
      HPLPQRLHT NSSIQPHII VVRKHNNKS CHHYHNTTL 
      PEDIPKATK DSKAKKIKS LSRSKREVL PFGTPVFVE 
      TAVFVDRDL FEHMKTNFP VETERELIR FVLAMINAV 
      QLLYHDPSL GRPVNFVLK RLEILKEEA AGLVRPPDI 
      DRFLSNFCI WQRTKNPVG DREPQHWDH ALILTGLDL 
      YVRGKHGKI SNQVVGLAP VAGMCTTTS SCTVNEGRH 
      FESVYVVAH EIGHNLGMR HDGPLADND CDPAGYIMS 
      PTLGSGKIT WSACSRRYL EKFLETSQS RCLLDHGSS 
      AGQLDHSAE GALPGERFD ADQQCMLKY GRGSRHSSQ 
      QPLDDVCRD LHCERERYT WTSHPALEG TMCSHNMWC 
      KGGRCISRG YPVSAAYST VSQTPSRHV EQNSGTWSK 
      WKPYSDCAS GCLFGEEGR LRSGSTGIM ASSRICNNP 
      RGSENYCEG QSKRYQACN AQQCLNVPR LTIREFAEQ 
      ICTRATDVD KDLTGLGMQ KLSSDPEEA CLVWCQKRN 
      GGTKSRGWT FPDGTVCQT RRSLYGKSS YCINGRCEE 
      FVCDSFEQA TFAQMPELC PVERTDNEL LWRTGLRGR 
      SNAVVKWKS ASGCHFNCI SPGTGVRLV MSKGKHAKS 
      SIQLCQPDQ YGCGRIKSP FQHASMICN KYKDRVGRL 
      SGIGMQIPP AVEDPDRPC RVACQDENI SHRYYLVNG 
      EEGWFPFGT DCSRSGAEK KAYCISGKC LEFGSDKTP 
      LSESEFTLP LLSRSRRSL ELNSTRVMA KLNQRQLEQ 
      IIKELNRTL SGNVKSDYR FDIDLSNPI HVDMDMESV 
      QYHTKKQST DFLNYT
    CDS sequence
    • >TC009506 RA
      atgcggggc atgttttcg cggtttttt atagccgcg 
      tggatatta ttcggaact gtgctcaat cttcaggta 
      acggaaacc aatttaagg ggcttatta accaatctg 
      gagtggcta tcgctcata ggaagttca aattcagga 
      tcgaaattc tcgatagtc tatccacat ttaacaacc 
      agtgacgat gccttagtc aaaaaactt gaaattgaa 
      acacccgaa aagaaattt atgtttgaa ctagtccct 
      aatcctgat ctcttatcc aaaaatttt ctagttttt 
      gaaacgttt gcgaacgga tcgtatcca atgcatttt 
      atcgatgat gatgctaga tgtcactac aggggagac 
      aaatcagca ctttgttta tgcaacgga attaaggga 
      cttttatat ttcgacaat gattcgctg tatatcatc 
      cacccatta ccacaaaga ttgcacacc aactcatca 
      attcaacct catattatc gtcgttcgc aaacacaat 
      aacaaatcc tgccatcac taccacaac acgacactg 
      cctgaagat attccaaaa gcaaccaaa gattcaaag 
      gccaaaaaa ataaaaagt ctgagccgt tcaaaaagg 
      gaagtcctc ccttttggc acacctgtt ttcgtagaa 
      actgccgtt tttgtagat agagactta tttgaacac 
      atgaaaact aattttcct gttgaaact gaacgcgag 
      ttgattcgg tttgttctt gccatgata aatgccgtg 
      caattgctt taccacgac ccaagccta ggaagaccg 
      gtcaatttt gtgttaaaa agacttgaa attttgaaa 
      gaagaagct gcaggtttg gttagacca cctgatatt 
      gatcggttt ctatcgaat ttttgcata tggcaaaga 
      actaaaaat cctgtaggc gatcgggaa ccgcaacat 
      tgggatcat gctttgatt ttgacaggg ttggacttg 
      tacgttagg ggcaaacat ggaaaaata tcgaatcaa 
      gttgtgggt ctagcaccg gtggctgga atgtgtaca 
      acaactagt agttgtact gttaatgaa ggaaggcat 
      tttgaaagt gtttatgtt gttgcgcac gaaattggg 
      cacaattta gggatgagg cacgatggg cctctggct 
      gataacgac tgtgatcct gctggttat atcatgtcg 
      ccaacttta ggaagtggt aaaattaca tggtctgcg 
      tgtagtaga agatatttg gaaaaattt ttagagaca 
      tcacaatca cggtgtttg cttgatcat gggagttca 
      gcaggtcag cttgaccat agtgctgaa ggggctttg 
      cctggcgaa cgattcgat gctgaccag caatgtatg 
      ttaaaatac ggtcgtggc agccgccac tcttcccaa 
      caacctctc gacgacgtc tgtcgcgac ctacactgc 
      gaacgagaa cgctacact tggacatcc catcctgct 
      ttggaaggc accatgtgc agccacaac atgtggtgt 
      aagggcggc agatgcatt tcccgaggc tatcctgtc 
      agtgctgct tacagcacg gtctctcaa actccttca 
      cgtcatgtg gaacaaaat tcgggaact tggagtaaa 
      tggaaacca tacagtgat tgtgcttct ggatgtctt 
      tttggcgaa gaagggcgg ttacgttcc ggaagcact 
      ggaattatg gcttctagt agaatttgc aacaatcct 
      cgtggtagc gaaaattac tgtgaaggg caaagtaag 
      agataccag gcttgcaac gcccagcag tgccttaat 
      gtgccacgg ctgactata agagagttt gctgagcaa 
      atctgcaca agggctacg gacgtagat aaggatttg 
      accggttta ggaatgcag aaactcagt tcggatcct 
      gaagaggct tgtcttgtg tggtgtcaa aaacgcaac 
      ggaggcaca aaaagccgt ggatggact tttcctgac 
      ggaacagtt tgccaaact cgacgctct ctttacgga 
      aagtcaagt tattgtatc aatggacgt tgtgaagag 
      tttgtctgt gactctttc gaacaagca accttcgct 
      caaatgccc gagttatgt ccagtcgaa cgcaccgac 
      aatgaactc ctctggaga accggactt agaggaaga 
      tccaatgca gttgtcaaa tggaaaagt gcgagtggt 
      tgtcatttt aactgtatt tccccagga actggtgtc 
      agattggtg atgagtaaa ggcaaacat gctaaatct 
      agcattcaa ttgtgtcaa ccggatcaa tatggttgt 
      ggacggata aaatctccc tttcaacat gcttccatg 
      atttgcaac aaatataaa gacagggtt gggaggctg 
      tcggggatc ggcatgcaa attccgcca gcagttgag 
      gatccagat agaccttgc cgagtggcc tgtcaggac 
      gagaacata tcgcaccgc tattacttg gttaatgga 
      gaagaaggc tggtttcct ttcggcact gattgctct 
      aggagcggt gcagaaaag aaagcttac tgcattagc 
      ggcaagtgt ttggaattt ggttctgat aaaactccg 
      ctatctgag agcgaattt actcttcca ttactgtca 
      aggagtcgc agatctctc gagttaaac agcacaaga 
      gtgatggca aaattaaat caacggcaa ctggagcaa 
      atcattaaa gaactgaat agaacttta tcaggaaac 
      gtaaaaagt gattatcgg tttgacatc gacttaagt 
      aatccgatt catgttgat atggacatg gaatccgtg 
      caatatcat actaaaaag caatctact gatttttta 
      aattatact taa
    mRNA sequence
    • >TC009506 RA
      tgcttacaa aaccttata cctatcata attacacgc 
      tggtgagga tggtaataa ttatgacca aatataaaa 
      attgtatca gtattcaag ttcgcaact agacaaagt 
      gcatggttc gttgcccgt gaagtggaa atggtaata 
      tctcctatc aaagccttc tctctgcat tttgcagac 
      tagttcagt gttgcttgc gaacagctg atgagtacc 
      gtgccgaag aagtccatc aagagcgcg taaaacgcg 
      atttttttt tcttcaaca aactgaaat gtgcaatga 
      aataaagaa aatgtgcga atgtaaaga cgagattga 
      ctgtggagg tattgaaat ggcgtttag tgtataaat 
      gggttttaa agaaacgcc aattggtca tgcggggca 
      tgttttcgc ggtttttta tagccgcgt ggatattat 
      tcggaactg tgctcaatc ttcaggtaa cggaaacca 
      atttaaggg gcttattaa ccaatctgg agtggctat 
      cgctcatag gaagttcaa attcaggat cgaaattct 
      cgatagtct atccacatt taacaacca gtgacgatg 
      ccttagtca aaaaacttg aaattgaaa cacccgaaa 
      agaaattta tgtttgaac tagtcccta atcctgatc 
      tcttatcca aaaattttc tagtttttg aaacgtttg 
      cgaacggat cgtatccaa tgcatttta tcgatgatg 
      atgctagat gtcactaca ggggagaca aatcagcac 
      tttgtttat gcaacggaa ttaagggac ttttatatt 
      tcgacaatg attcgctgt atatcatcc acccattac 
      cacaaagat tgcacacca actcatcaa ttcaacctc 
      atattatcg tcgttcgca aacacaata acaaatcct 
      gccatcact accacaaca cgacactgc ctgaagata 
      ttccaaaag caaccaaag attcaaagg ccaaaaaaa 
      taaaaagtc tgagccgtt caaaaaggg aagtcctcc 
      cttttggca cacctgttt tcgtagaaa ctgccgttt 
      ttgtagata gagacttat ttgaacaca tgaaaacta 
      attttcctg ttgaaactg aacgcgagt tgattcggt 
      ttgttcttg ccatgataa atgccgtgc aattgcttt 
      accacgacc caagcctag gaagaccgg tcaattttg 
      tgttaaaaa gacttgaaa ttttgaaag aagaagctg 
      caggtttgg ttagaccac ctgatattg atcggtttc 
      tatcgaatt tttgcatat ggcaaagaa ctaaaaatc 
      ctgtaggcg atcgggaac cgcaacatt gggatcatg 
      ctttgattt tgacagggt tggacttgt acgttaggg 
      gcaaacatg gaaaaatat cgaatcaag ttgtgggtc 
      tagcaccgg tggctggaa tgtgtacaa caactagta 
      gttgtactg ttaatgaag gaaggcatt ttgaaagtg 
      tttatgttg ttgcgcacg aaattgggc acaatttag 
      ggatgaggc acgatgggc ctctggctg ataacgact 
      gtgatcctg ctggttata tcatgtcgc caactttag 
      gaagtggta aaattacat ggtctgcgt gtagtagaa 
      gatatttgg aaaaatttt tagagacat cacaatcac 
      ggtgtttgc ttgatcatg ggagttcag caggtcagc 
      ttgaccata gtgctgaag gggctttgc ctggcgaac 
      gattcgatg ctgaccagc aatgtatgt taaaatacg 
      gtcgtggca gccgccact cttcccaac aacctctcg 
      acgacgtct gtcgcgacc tacactgcg aacgagaac 
      gctacactt ggacatccc atcctgctt tggaaggca 
      ccatgtgca gccacaaca tgtggtgta agggcggca 
      gatgcattt cccgaggct atcctgtca gtgctgctt 
      acagcacgg tctctcaaa ctccttcac gtcatgtgg 
      aacaaaatt cgggaactt ggagtaaat ggaaaccat 
      acagtgatt gtgcttctg gatgtcttt ttggcgaag 
      aagggcggt tacgttccg gaagcactg gaattatgg 
      cttctagta gaatttgca acaatcctc gtggtagcg 
      aaaattact gtgaagggc aaagtaaga gataccagg 
      cttgcaacg cccagcagt gccttaatg tgccacggc 
      tgactataa gagagtttg ctgagcaaa tctgcacaa 
      gggctacgg acgtagata aggatttga ccggtttag 
      gaatgcaga aactcagtt cggatcctg aagaggctt 
      gtcttgtgt ggtgtcaaa aacgcaacg gaggcacaa 
      aaagccgtg gatggactt ttcctgacg gaacagttt 
      gccaaactc gacgctctc tttacggaa agtcaagtt 
      attgtatca atggacgtt gtgaagagt ttgtctgtg 
      actctttcg aacaagcaa ccttcgctc aaatgcccg 
      agttatgtc cagtcgaac gcaccgaca atgaactcc 
      tctggagaa ccggactta gaggaagat ccaatgcag 
      ttgtcaaat ggaaaagtg cgagtggtt gtcatttta 
      actgtattt ccccaggaa ctggtgtca gattggtga 
      tgagtaaag gcaaacatg ctaaatcta gcattcaat 
      tgtgtcaac cggatcaat atggttgtg gacggataa 
      aatctccct ttcaacatg cttccatga tttgcaaca 
      aatataaag acagggttg ggaggctgt cggggatcg 
      gcatgcaaa ttccgccag cagttgagg atccagata 
      gaccttgcc gagtggcct gtcaggacg agaacatat 
      cgcaccgct attacttgg ttaatggag aagaaggct 
      ggtttcctt tcggcactg attgctcta ggagcggtg 
      cagaaaaga aagcttact gcattagcg gcaagtgtt 
      tggaatttg gttctgata aaactccgc tatctgaga 
      gcgaattta ctcttccat tactgtcaa ggagtcgca 
      gatctctcg agttaaaca gcacaagag tgatggcaa 
      aattaaatc aacggcaac tggagcaaa tcattaaag 
      aactgaata gaactttat caggaaacg taaaaagtg 
      attatcggt ttgacatcg acttaagta atccgattc 
      atgttgata tggacatgg aatccgtgc aatatcata 
      ctaaaaagc aatctactg attttttaa attatactt 
      aaagcctta ggtagtaaa aaaatgtga ttattatga 
      tgttccatc ttagattat taattagat acatctatg 
      tgaaagaaa aaatatatc tttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY