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Gene information
General information
TC009503 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 190801..193940
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009503
    iBeetle screen iB_01566

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009503 RA
      MSGEASPDK MDISETSDT SSHEISNSS QVLRFECEQ 
      EKPDLDNAT SSIKSVSLN ATNSHPLMY TCTSPISPD 
      FPDLIPEKP KSKHGNYNT IKQETGGEE LEGCLHFNL 
      PPLNEYNEL PDFPESPSA LKKSLMYDL PLEVTTNTE 
      LSDTENDKS VYEAHLSSN LQNVCLTMN EKEGNLNFL 
      AHKKRSNKL RRVKVMETE ASEESDISS LDSISESLK 
      SEHVFTGGQ LEIGVEEQD NSSEPIEPL SAADERRHA 
      RHWQRMVLP GGEQRTIDM RVIEPYKRV LSHGGYLRA 
      GGHTAIVIF SACYLPDRS RVDYVYVMD NLFLYILWT 
      LERLVTDDY VLVYLHGGA TKLPAFSWL KKCYQMVGR 
      KLRKNLSHL YLVHPTLWI KTMLFMAKP FISSKFYRK 
      ISYVSSLKE LMVRVPLEA AAIPDKVKA YDSLHCVT
    CDS sequence
    • >TC009503 RA
      atgagcggt gaggcaagt cctgacaaa atggacatt 
      tcggaaaca tctgacacg tccagtcac gaaataagc 
      aattctagt caagttttg aggttcgaa tgcgaacaa 
      gaaaagccc gatttggat aacgcaaca tcttcaatc 
      aaaagcgtg tctttaaac gcaacaaat tcacatccg 
      ttgatgtac acatgcact tctcccatt tccccagac 
      ttccccgac ttgattccc gaaaaaccg aaaagcaaa 
      cacgggaat tataatacc atcaaacag gagacgggg 
      ggtgaagag ctggaaggg tgtttacac tttaatttg 
      cccccttta aacgagtac aatgaatta ccagatttt 
      cctgaaagt ccctcagct ttaaaaaaa tcgctaatg 
      tatgatttg cctttagag gttaccacc aatactgaa 
      ttgagtgat actgaaaat gataagtct gtttatgaa 
      gcacacctt agttctaat ttgcaaaac gtatgtctc 
      actatgaat gaaaaggaa ggtaacttg aattttttg 
      gctcacaaa aagcgcagc aataaactc cgccgcgtg 
      aaagtaatg gaaacggaa gcgagcgaa gagagcgat 
      atttcttca cttgacagc atttccgag agtctaaaa 
      tcggagcac gtctttacc ggaggacag ttggaaatt 
      ggtgtcgaa gagcaagac aattcttct gaacccatt 
      gagccttta agcgcagcc gatgaacga agacatgcg 
      cgtcattgg cagcggatg gtgctgcca ggaggcgaa 
      caacgaact atagacatg cgggtgatt gaaccatac 
      aaaagggtg ctttcccat ggagggtat cttcgagct 
      gggggacat acagcgatc gtcattttt tccgcttgt 
      tacctccct gatcgatcc agagttgac tatgtttac 
      gtcatggat aatctcttt ttatatata ctttggact 
      ctcgagcgg ttagtgacc gatgattat gtcttggtg 
      tatctacac ggtggtgca acgaaattg ccagcgttc 
      agttggctg aaaaaatgc taccaaatg gtagggcgc 
      aagttgcgt aaaaatttg agtcatttg tatcttgtg 
      catccaacg ctttggatc aagacgatg ctgttcatg 
      gcaaagccg tttatcagc tcaaagttc tatcgaaaa 
      ataagttac gtatcgtcg ctgaaggag ttgatggtg 
      cgtgttcct ctcgaagcg gctgcaatt cctgataaa 
      gtcaaagct tatgacagt ttacactgt gttacgtag
    mRNA sequence
    • >TC009503 RA
      tgcgatttt aaaaatggc ttaaatctt ggttgtatc 
      taaagtgga acaaacagg cattaatgt aatcaaaag 
      actgtcttc gaacaattt ggaatcaca aatgagcgg 
      tgaggcaag tcctgacaa aatggacat ttcggaaac 
      atctgacac gtccagtca cgaaataag caattctag 
      tcaagtttt gaggttcga atgcgaaca agaaaagcc 
      cgatttgga taacgcaac atcttcaat caaaagcgt 
      gtctttaaa cgcaacaaa ttcacatcc gttgatgta 
      cacatgcac ttctcccat ttccccaga cttccccga 
      cttgattcc cgaaaaacc gaaaagcaa acacgggaa 
      ttataatac catcaaaca ggagacggg gggtgaaga 
      gctggaagg gtgtttaca ctttaattt gcccccttt 
      aaacgagta caatgaatt accagattt tcctgaaag 
      tccctcagc tttaaaaaa atcgctaat gtatgattt 
      gcctttaga ggttaccac caatactga attgagtga 
      tactgaaaa tgataagtc tgtttatga agcacacct 
      tagttctaa tttgcaaaa cgtatgtct cactatgaa 
      tgaaaagga aggtaactt gaatttttt ggctcacaa 
      aaagcgcag caataaact ccgccgcgt gaaagtaat 
      ggaaacgga agcgagcga agagagcga tatttcttc 
      acttgacag catttccga gagtctaaa atcggagca 
      cgtctttac cggaggaca gttggaaat tggtgtcga 
      agagcaaga caattcttc tgaacccat tgagccttt 
      aagcgcagc cgatgaacg aagacatgc gcgtcattg 
      gcagcggat ggtgctgcc aggaggcga acaacgaac 
      tatagacat gcgggtgat tgaaccata caaaagggt 
      gctttccca tggagggta tcttcgagc tgggggaca 
      tacagcgat cgtcatttt ttccgcttg ttacctccc 
      tgatcgatc cagagttga ctatgttta cgtcatgga 
      taatctctt tttatatat actttggac tctcgagcg 
      gttagtgac cgatgatta tgtcttggt gtatctaca 
      cggtggtgc aacgaaatt gccagcgtt cagttggct 
      gaaaaaatg ctaccaaat ggtagggcg caagttgcg 
      taaaaattt gagtcattt gtatcttgt gcatccaac 
      gctttggat caagacgat gctgttcat ggcaaagcc 
      gtttatcag ctcaaagtt ctatcgaaa aataagtta 
      cgtatcgtc gctgaagga gttgatggt gcgtgttcc 
      tctcgaagc ggctgcaat tcctgataa agtcaaagc 
      ttatgacag tttacactg tgttacgta gtttaaaac 
      acaaccgtc atgccttgc ctatctaaa cattcctaa 
      agctttacc ttattgtac ttaaaccag gctttggtt 
      ttgatacat cgaattatt tatatattt atatcgttt 
      tgtgaattt gttatttta ttgattttc tttattttt 
      ttaaaagaa tgttgagaa gtggtttac gtgaagtaa 
      tgtaggtat gtcattagc aatgtgtct ctaggatgt 
      aataaagag ggtattgtt ggtaatttt ctctttttt 
      aaatgtttt aaataaact gtcatattc aagcgtcat 
      tcatatacg acagatcgg aaaatgtct tggaaagaa 
      aacagattt aaaaccaca tacaataaa aaaatagat 
      cgaaaaaaa tgaacatct tttaacgga gatgctgaa 
      gtctttaag cttgaaaga ggaaccttg tatgtgaaa 
      atttacgag gaagttgaa caaaaataa tataattta 
      cgagaaaat aaaggtttt tcaagtacg gtgtaagtt 
      attgttgaa aaaaaaata attagaaaa tttgttatt 
      ttcgtgctg cagaaaaat cttttctgt gaagtgacg 
      tcaaagata aaatttcca gaaagtgcg taataactt 
      ggttccttg gaatctaaa cggaagtga gtgacacga 
      cataagagt gaaaattag caatagtaa ataaacagt 
      aaattattt agcaaattt ttgtgagtt gaaaaaatc 
      gctttatgc gtaattttt ttttcattg ccaaataag 
      gataataaa aatgatttg caatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY