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Gene information
General information
TC009502 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid46: 73801..76879
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009502
    iBeetle screen iB_01565
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: >5 - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009502 RA
      MGIIITKFR KKKTTYEVL EKLEKEIQS IEEFRRSTE 
      QTQKKMVGR FIFISICVY CVTALILYF YFFPASAYE 
      RIIYIIPLI ILPMLLILI KRTLSWYYS RKIRKNQKK 
      LSTLKEEKK KILEEVMDK ETYKVAKTI LEKFAPEQV 
      IKRNPAFDN QSTPVKSGP LVTLTPTNQ SHSGLRYRG 
      QLSTAAPNR TLGSTMTPI SRQALVPLT TPSLLSRPS 
      QGGRPSIPS TPLPLPRAI LPRERTVLD KMVDYLVGD 
      GPSNRFALI CKNCAAHNG MAIKEEFEY LSFRCCYCL 
      TFNPSRKKR PIAPKLDNT LSIKDKRSA ISDSDSDSD 
      TAEPIITEP LTEHNVTES EKLSDYDVK NSDTESTAM 
      EVNTVTPSK EKSKNEEVE DDATETQD
    CDS sequence
    • >TC009502 RA
      atgggtatc attattacg aaatttcgg aaaaagaaa 
      accacgtat gaagtgctg gaaaagctc gaaaaggaa 
      atccaatca atagaagag tttcgcaga agcaccgaa 
      caaactcag aagaaaatg gttgggcgt ttcattttc 
      atctcaatt tgcgtgtat tgtgtgaca gctttaata 
      ttgtacttt tatttcttc ccagcgtcg gcctacgaa 
      agaattatt tacataata ccactaatt atactcccg 
      atgttacta attctaata aaacggact ttgtcatgg 
      tattacagc agaaagatt aggaaaaac caaaagaaa 
      ttgtccact ttgaaagaa gagaaaaag aaaattttg 
      gaggaagtt atggacaag gaaacttac aaagttgcc 
      aaaactatt ttagaaaaa ttcgcacct gaacaagtc 
      atcaagcga aatccggct tttgacaac caaagcacc 
      cctgtgaaa agcggacct ttggtcacc ctcacgcca 
      acaaatcag agtcactca ggtttacga tatcgaggt 
      cagttgtca acagccgcc cctaatcgt actttaggc 
      tctacaatg acccccata tcaagacaa gctctggtg 
      cctttaacc actccttca ctgctttca cgaccttct 
      caaggaggg cgcccttca atccctagc acccctttg 
      ccactgcca agggccatc ttgcctagg gaacgtaca 
      gttctggat aagatggtg gactacctc gttggagat 
      ggtccttct aacagattt gcactgatt tgtaagaac 
      tgtgctgct cataatggt atggctatt aaagaagag 
      ttcgagtat ttgtcgttt cgttgttgt tattgtttg 
      acgtttaac ccgtctcgt aaaaagcgt cccattgct 
      ccaaaatta gacaatacg ctgtcgatt aaagacaag 
      agatctgcc atatcagat tctgattca gattcggac 
      acagcggaa ccgattata accgaacca ctgactgaa 
      cataacgtc accgagtcg gaaaagctt tcagattat 
      gatgtaaaa aattcagac acggaatca acagcgatg 
      gaagtgaat acagttaca ccaagcaag gaaaaaagt 
      aaaaatgag gaagttgaa gatgatgct actgaaaca 
      caagactga
    mRNA sequence
    • >TC009502 RA
      actctccaa agtccggtc ctggacaca tttcaagaa 
      tcagtacaa atccgtgaa tatgacagc gaacaagcg 
      atagcaaaa attctagtt tttttaagc tacaaaatt 
      caataatca attcgggtt aagtcgaca ccgaaacct 
      ggctctcca tgctatcaa tgttataat tgatacaaa 
      ttatcgtat ttaattatt atttattta tttataagt 
      aattcacaa acatgggta tcattatta cgaaatttc 
      ggaaaaaga aaaccacgt atgaagtgc tggaaaagc 
      tcgaaaagg aaatccaat caatagaag agtttcgca 
      gaagcaccg aacaaactc agaagaaaa tggttgggc 
      gtttcattt tcatctcaa tttgcgtgt attgtgtga 
      cagctttaa tattgtact tttatttct tcccagcgt 
      cggcctacg aaagaatta tttacataa taccactaa 
      ttatactcc cgatgttac taattctaa taaaacgga 
      ctttgtcat ggtattaca gcagaaaga ttaggaaaa 
      accaaaaga aattgtcca ctttgaaag aagagaaaa 
      agaaaattt tggaggaag ttatggaca aggaaactt 
      acaaagttg ccaaaacta ttttagaaa aattcgcac 
      ctgaacaag tcatcaagc gaaatccgg cttttgaca 
      accaaagca cccctgtga aaagcggac ctttggtca 
      ccctcacgc caacaaatc agagtcact caggtttac 
      gatatcgag gtcagttgt caacagccg cccctaatc 
      gtactttag gctctacaa tgaccccca tatcaagac 
      aagctctgg tgcctttaa ccactcctt cactgcttt 
      cacgacctt ctcaaggag ggcgccctt caatcccta 
      gcacccctt tgccactgc caagggcca tcttgccta 
      gggaacgta cagttctgg ataagatgg tggactacc 
      tcgttggag atggtcctt ctaacagat ttgcactga 
      tttgtaaga actgtgctg ctcataatg gtatggcta 
      ttaaagaag agttcgagt atttgtcgt ttcgttgtt 
      gttattgtt tgacgttta acccgtctc gtaaaaagc 
      gtcccattg ctccaaaat tagacaata cgctgtcga 
      ttaaagaca agagatctg ccatatcag attctgatt 
      cagattcgg acacagcgg aaccgatta taaccgaac 
      cactgactg aacataacg tcaccgagt cggaaaagc 
      tttcagatt atgatgtaa aaaattcag acacggaat 
      caacagcga tggaagtga atacagtta caccaagca 
      aggaaaaaa gtaaaaatg aggaagttg aagatgatg 
      ctactgaaa cacaagact gacacatat tacaaacaa 
      aaattcttg tatgtacga taagtaaaa tcaagaaat 
      gtttaccca ttttttttg taatatatt tacgactta 
      tttattgct gtgtataca tatgtagaa aagagtggt 
      ttacgtgta aataatttt gatttttgt ggttattcg 
      atttttgac attctattc tgcacagaa tgagtaaca 
      gtggttttg gataatgtt ggactgttt tcgtttata 
      ataggtgcc tttctgcat tttataata aagcttgga 
      atttccaaa ccgttacag atacctgtt ttccaacct 
      tggacctat cacattttt actacattc ttgtatttt 
      tgtataata acgttgtgt gttaattaa attaaatta 
      ttactaata aatatttgc ttatttc