Status
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Gene information
General information
TC009495 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 281296..285664
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009495
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01564

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 1

    egg chamber mostly not present - Number of animals showing the phenotype: 1

    egg chamber potentiallydegenerated - Number of animals showing the phenotype: 2

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009495 RA
      MGDLRFDGR VVVVTGAGA GLGRAYALL FGSRGAKVV 
      VNDLGGGRH GDGKSSSAA DAVVEEIKR NGGTAVADY 
      NSVVDGEKV IKTALDNFG RVDVLINNA GILRDKSFA 
      RISDQDWDL VHAVHLKGS FKTTQAAFP IFKKQGYGR 
      IIMTSSNSG LYGNFGQAN YSAAKMGLV GLASTIAIE 
      GAKNNIHCN VIVPTAASR LTEDILPPD LYAELKPEL 
      IAPVVAYLC HESCTENGS IIESAAGWA GKCQIIRSN 
      GSLLRTKIT DGVSVENVR DKWQKVTSM SNAEHLDSI 
      QEATGALMT SLDSLRSGE VKDEEVDVY NFTQKDIIL 
      YALGVGASL KNPNELKFI YENDEDFSA LPTFYIMPA 
      MQAMFTSSK LESAIPGKT VSLAQILHG EQYIEFLGE 
      VPKEGKLFS KNSIVEVLD KGSGAAIVQ NIETFDEQG 
      NLLIRNQTV AFVVGAGNF GGSRTGTKA IPCQSKPPR 
      VPDLSLTDK TTIDQAALY RLSGDTNPL HIDPNMAVV 
      AGFKQPILH GLCTLGFSI RLLVRAYAG GDPSFVKAC 
      KARFMKPVI PGQTLRVDF WREGSRIHF ETSVVETNT 
      VVIGGAYVD LNSVKVMEP INNSVSAPA LESDAVFQF 
      IIDQVKADP NKAKSIGGV FLYKITKNG KEAKQWTMD 
      LKNAKVYEG APEGKPSTT LTIADEDFM LLAQGKLQP 
      QAAFMKGKL KITGNIMLA QKLAPILKA NAKL
    CDS sequence
    • >TC009495 RA
      atgggtgat ctaagattt gatgggaga gtggtcgtg 
      gtcacgggg gctggagct gggctaggc cgagcctat 
      gccctttta tttggctct cggggggcc aaagttgta 
      gtcaacgat ttagggggc ggtagacat ggtgatggt 
      aaatcatca agtgcagca gacgctgtg gttgaagaa 
      attaaaagg aatgggggc acagcagtt gccgattac 
      aattcggtt gttgatgga gaaaaggtc attaaaacg 
      gcgcttgat aatttcgga cgtgttgat gttttgatt 
      aacaatgcg gggattttg agagacaag agttttgcg 
      agaatatct gaccaggat tgggattta gttcatgcg 
      gtgcatttg aaaggcagt tttaagacc actcaagct 
      gcctttccc atttttaag aaacaaggg tatgggaga 
      attataatg acttcttca aattctgga ctttacggt 
      aattttggt caagcgaat tattccgct gctaaaatg 
      ggtcttgtt ggtttggcc agtactatt gcaattgaa 
      ggggcaaag aataatatt cattgcaat gtcattgtt 
      ccaacagct gcgagtaga ctgactgag gatattttg 
      ccacctgat ctctacgct gaactcaaa ccggagcta 
      attgcacct gttgtggcc tacttatgt cacgaaagt 
      tgcaccgaa aacggctct ataattgaa tcagcggct 
      ggttgggcc ggaaagtgc caaataatt cgttcaaat 
      ggtagtctt ttacgtacg aaaatcact gacggtgtt 
      tcggttgaa aacgttcgt gataagtgg caaaaagtc 
      acttcaatg tccaatgct gaacatttg gactcaatc 
      caagaagct actggtgct ttgatgacg tcacttgac 
      agtcttcgg tcaggtgaa gtcaaagac gaagaagtt 
      gacgtttat aactttacc cagaaagat atcatcttg 
      tatgcactt ggagtgggc gcgtctttg aaaaatcca 
      aacgaactg aaatttatc tacgaaaac gacgaagat 
      tttagtgcc ttaccgaca ttttacata atgccggca 
      atgcaagca atgttcact tcttccaaa cttgagtca 
      gcaattccc ggaaaaacg gtcagctta gctcagatt 
      ttacacggc gagcaatat atcgagttt ttgggagaa 
      gtccccaaa gaaggaaaa ttattttca aagaactca 
      atcgttgaa gttttggat aagggatcg ggagctgca 
      atcgttcaa aacattgaa acgtttgac gagcaagga 
      aatttacta atccgcaac caaactgtc gcttttgtt 
      gtgggtgct ggcaatttt ggtggttct agaactggt 
      actaaagca atcccttgc caaagcaag ccaccccga 
      gtacctgat ttgagtctc actgataaa actacaatt 
      gatcaagct gccctctat cggctttcg ggtgataca 
      aacccgctt catattgat ccgaatatg gcagtggtg 
      gctggattt aaacaaccg attttgcac ggtttatgc 
      actttggga ttttcaatc aggttgtta gtgagggct 
      tatgctggt ggtgatcca agttttgtt aaagcttgc 
      aaggccagg tttatgaaa ccggtgatt cccggacaa 
      acgctcaga gttgatttt tggagggaa ggcagtaga 
      attcatttc gagacaagt gttgtagag acaaatacg 
      gtggtgata ggaggagcg tacgttgat ttgaattcg 
      gtcaaagta atggaacca atcaacaat tccgtctcg 
      gctcctgcc ttggaaagt gatgcagtt ttccagttt 
      ataatcgac caagtgaaa gccgatccg aataaagcc 
      aagtcaatt ggaggagta tttttgtat aaaatcacc 
      aaaaatggc aaagaagcc aaacaatgg acaatggat 
      ttgaaaaac gctaaagtt tacgagggg gcgcctgag 
      ggtaaacca agtaccact ttgaccatt gccgatgaa 
      gattttatg cttctggca caagggaag ttgcaacca 
      caagccgct ttcatgaag ggaaaactc aaaattacc 
      ggaaatatc atgctggcg cagaaattg gctccaatt 
      ctcaaagcc aacgccaaa ctgtaa
    mRNA sequence
    • >TC009495 RA
      gttatgtaa tacagtgta ttttcttat ttttaattt 
      aaattacaa ttcgatgaa ttgactatt cctgattag 
      tacaataac taccacaat tctgtaaat ttacgaaaa 
      ttagctaat tagtgtaat tgccaagtg aaacgtcaa 
      aaagaaagt tgttcttct agaaaaaac acctcaagt 
      agtggagaa ggggcaata ataaacttt ttatctagt 
      ttatatttt cattaacgt tgtcaatac gcatgacaa 
      gacactttt caataattt gctcaaagt tcagtcgac 
      taaattagt gagcgttgt cgtgttgct aaaccataa 
      tttcgccaa aaaatgggt gatctaaga tttgatggg 
      agagtggtc gtggtcacg ggggctgga gctgggcta 
      ggccgagcc tatgccctt ttatttggc tctcggggg 
      gccaaagtt gtagtcaac gatttaggg ggcggtaga 
      catggtgat ggtaaatca tcaagtgca gcagacgct 
      gtggttgaa gaaattaaa aggaatggg ggcacagca 
      gttgccgat tacaattcg gttgttgat ggagaaaag 
      gtcattaaa acggcgctt gataatttc ggacgtgtt 
      gatgttttg attaacaat gcggggatt ttgagagac 
      aagagtttt gcgagaata tctgaccag gattgggat 
      ttagttcat gcggtgcat ttgaaaggc agttttaag 
      accactcaa gctgccttt cccattttt aagaaacaa 
      gggtatggg agaattata atgacttct tcaaattct 
      ggactttac ggtaatttt ggtcaagcg aattattcc 
      gctgctaaa atgggtctt gttggtttg gccagtact 
      attgcaatt gaaggggca aagaataat attcattgc 
      aatgtcatt gttccaaca gctgcgagt agactgact 
      gaggatatt ttgccacct gatctctac gctgaactc 
      aaaccggag ctaattgca cctgttgtg gcctactta 
      tgtcacgaa agttgcacc gaaaacggc tctataatt 
      gaatcagcg gctggttgg gccggaaag tgccaaata 
      attcgttca aatggtagt cttttacgt acgaaaatc 
      actgacggt gtttcggtt gaaaacgtt cgtgataag 
      tggcaaaaa gtcacttca atgtccaat gctgaacat 
      ttggactca atccaagaa gctactggt gctttgatg 
      acgtcactt gacagtctt cggtcaggt gaagtcaaa 
      gacgaagaa gttgacgtt tataacttt acccagaaa 
      gatatcatc ttgtatgca cttggagtg ggcgcgtct 
      ttgaaaaat ccaaacgaa ctgaaattt atctacgaa 
      aacgacgaa gattttagt gccttaccg acattttac 
      ataatgccg gcaatgcaa gcaatgttc acttcttcc 
      aaacttgag tcagcaatt cccggaaaa acggtcagc 
      ttagctcag attttacac ggcgagcaa tatatcgag 
      tttttggga gaagtcccc aaagaagga aaattattt 
      tcaaagaac tcaatcgtt gaagttttg gataaggga 
      tcgggagct gcaatcgtt caaaacatt gaaacgttt 
      gacgagcaa ggaaattta ctaatccgc aaccaaact 
      gtcgctttt gttgtgggt gctggcaat tttggtggt 
      tctagaact ggtactaaa gcaatccct tgccaaagc 
      aagccaccc cgagtacct gatttgagt ctcactgat 
      aaaactaca attgatcaa gctgccctc tatcggctt 
      tcgggtgat acaaacccg cttcatatt gatccgaat 
      atggcagtg gtggctgga tttaaacaa ccgattttg 
      cacggttta tgcactttg ggattttca atcaggttg 
      ttagtgagg gcttatgct ggtggtgat ccaagtttt 
      gttaaagct tgcaaggcc aggtttatg aaaccggtg 
      attcccgga caaacgctc agagttgat ttttggagg 
      gaaggcagt agaattcat ttcgagaca agtgttgta 
      gagacaaat acggtggtg ataggagga gcgtacgtt 
      gatttgaat tcggtcaaa gtaatggaa ccaatcaac 
      aattccgtc tcggctcct gccttggaa agtgatgca 
      gttttccag tttataatc gaccaagtg aaagccgat 
      ccgaataaa gccaagtca attggagga gtatttttg 
      tataaaatc accaaaaat ggcaaagaa gccaaacaa 
      tggacaatg gatttgaaa aacgctaaa gtttacgag 
      ggggcgcct gagggtaaa ccaagtacc actttgacc 
      attgccgat gaagatttt atgcttctg gcacaaggg 
      aagttgcaa ccacaagcc gctttcatg aagggaaaa 
      ctcaaaatt accggaaat atcatgctg gcgcagaaa 
      ttggctcca attctcaaa gccaacgcc aaactgtaa 
      tttggtttt gtgtacgag gcacattcc gttttttgt 
      gtcactggt ttatatttt ttatgtatg gtgaattgt 
      gtataatta ttttaagtt tcaaaagtt ttgtattct 
      gttattgtg gacttgttt aaatatatt tctttacca 
      aacgagctt tttgtagat tttttcaag ccaaattgg 
      ttaagcaag aatgaagtc tatcaagta gttggagaa 
      ataaaatta acaacgttg ttaagagtt gttgctata 
      gcaataagt ctaaccaat aaaactaca gttaaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY