Status
Please wait ...
Gene information
General information
TC009494 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid46: 165519..168633
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009494
    iBeetle screen iB_01563
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009494 RA
      MVQNDSSNS GDEGSPPVA APNPAKGIP LGKYRLVGW 
      GVDTTGRRL IDEICQIAA YSPSSQFSQ YIMPFSDLN 
      PSYRRKHSI RVVNTGRYR MLKDMRSNK FVKTKSDVA 
      ALTDFLEWL EKVQGDDAH DGIILIYHE IRKASPGML 
      LEVLRRYNL LERFAKIVK GFANGFNIA QAKCAKTTK 
      SFSLRVMAK VLLNREDED FSSAVDRAR VSYEIAAHL 
      AQGERQDLD EKKPTGTEP EIINFVCPF VNPISAEED 
      EIAQFKVLL ERQNTFRPV FGALLRASR PERQHATHL 
      RRLLAENNI NYDKLKQAY EDGAKDGLD KVLKSEVAN 
      AKETELSEL LDILDCFFD PEKKAVQPK PRYYQNNNN 
      RKPRGTRKS FNKDRNSES TSSPNASSD NATEKTGTE 
      SEKEKTVEV Q
    CDS sequence
    • >TC009494 RA
      atggtccag aacgacagt tcgaattca ggcgacgag 
      ggctcgccc ccagtagcc gcccccaac ccggccaag 
      ggcattcct ttggggaag taccgcctc gtgggttgg 
      ggggtggac accaccgga cgccgcctc atcgacgaa 
      atctgccaa atcgccgcc tacagccct tcaagtcaa 
      ttttcccaa tacatcatg cccttctca gatttaaac 
      ccttcgtac cgtcgcaaa cacagcatc cgtgtagtc 
      aacacgggt cgctatcgc atgctcaaa gacatgcga 
      tcgaataaa ttcgtcaag accaagagc gacgtggcg 
      gcgcttacc gacttcctc gaatggctg gagaaggtg 
      caaggggat gacgcccac gacggcatc atcctcatc 
      taccacgag atacgcaaa gcatcacca ggcatgctc 
      ctggaggtc ctacgcagg tacaacctc ctggagcgt 
      ttcgctaaa attgtcaaa ggctttgcg aacggtttt 
      aacattgct caagcaaaa tgcgccaaa accaccaaa 
      tcgttcagt ctgagagtc atggctaag gtgctcctc 
      aaccgcgaa gatgaagat ttttccagt gctgttgac 
      cgagcgagg gtttcgtat gaaattgcg gcgcattta 
      gcgcaaggc gaacgccag gatttggac gagaagaaa 
      cccacagga accgaaccc gaaattatt aattttgtt 
      tgcccgttt gttaatcct atttcagca gaagaggat 
      gaaattgca cagtttaag gtgttgcta gaaaggcaa 
      aacactttc cgccctgtt tttggtgcc ttattgaga 
      gcgagtcgc cccgaaaga cagcatgca acgcatttg 
      agacgactt ttggccgaa aataatatt aattatgat 
      aaattgaag caggcgtat gaggatggt gcaaaggat 
      ggattggat aaggtgcta aaaagtgag gtagcgaac 
      gccaaagag actgaactg agcgagctg ttggacatt 
      ttggattgt tttttcgat ccggagaaa aaggctgtg 
      cagcccaag ccacggtat tatcaaaac aacaacaat 
      aggaaacct cgaggtaca agaaaatcg ttcaacaaa 
      gataggaat tcagaatcg acgagtagt ccaaacgcc 
      tcgtctgat aatgcgact gagaaaact ggaaccgaa 
      tctgaaaaa gagaagact gtagaagtg caataa
    mRNA sequence
    • >TC009494 RA
      ctacacacg tgcaaaatg cattttcca caaaatcac 
      agctaatgt tactagtgt tctcacacc tatattttc 
      tagtctcaa actttccca acacctcaa ccctttacc 
      caagaacaa acttgtgaa ccagcatgg tccagaacg 
      acagttcga attcaggcg acgagggct cgcccccag 
      tagccgccc ccaacccgg ccaagggca ttcctttgg 
      ggaagtacc gcctcgtgg gttgggggg tggacacca 
      ccggacgcc gcctcatcg acgaaatct gccaaatcg 
      ccgcctaca gcccttcaa gtcaatttt cccaataca 
      tcatgccct tctcagatt taaaccctt cgtaccgtc 
      gcaaacaca gcatccgtg tagtcaaca cgggtcgct 
      atcgcatgc tcaaagaca tgcgatcga ataaattcg 
      tcaagacca agagcgacg tggcggcgc ttaccgact 
      tcctcgaat ggctggaga aggtgcaag gggatgacg 
      cccacgacg gcatcatcc tcatctacc acgagatac 
      gcaaagcat caccaggca tgctcctgg aggtcctac 
      gcaggtaca acctcctgg agcgtttcg ctaaaattg 
      tcaaaggct ttgcgaacg gttttaaca ttgctcaag 
      caaaatgcg ccaaaacca ccaaatcgt tcagtctga 
      gagtcatgg ctaaggtgc tcctcaacc gcgaagatg 
      aagattttt ccagtgctg ttgaccgag cgagggttt 
      cgtatgaaa ttgcggcgc atttagcgc aaggcgaac 
      gccaggatt tggacgaga agaaaccca caggaaccg 
      aacccgaaa ttattaatt ttgtttgcc cgtttgtta 
      atcctattt cagcagaag aggatgaaa ttgcacagt 
      ttaaggtgt tgctagaaa ggcaaaaca ctttccgcc 
      ctgtttttg gtgccttat tgagagcga gtcgccccg 
      aaagacagc atgcaacgc atttgagac gacttttgg 
      ccgaaaata atattaatt atgataaat tgaagcagg 
      cgtatgagg atggtgcaa aggatggat tggataagg 
      tgctaaaaa gtgaggtag cgaacgcca aagagactg 
      aactgagcg agctgttgg acattttgg attgttttt 
      tcgatccgg agaaaaagg ctgtgcagc ccaagccac 
      ggtattatc aaaacaaca acaatagga aacctcgag 
      gtacaagaa aatcgttca acaaagata ggaattcag 
      aatcgacga gtagtccaa acgcctcgt ctgataatg 
      cgactgaga aaactggaa ccgaatctg aaaaagaga 
      agactgtag aagtgcaat aattgtgcc tttgacatt 
      tttcattga tttttttta caacacttc tttcgttgc 
      tttattaat cagttaaga attttttta ttttatttg 
      taattatta atgcgtaat ttaacgttt ttttttaac 
      gaaaggtgt gttactata aggtgttaa tattttcgg 
      agtttattt gtggataaa atctttaag tttatagga 
      gtcatgttg atcgtgata atgagataa tatattgca 
      attattgta tattaagtt tgataatgg gtgattttt 
      atgtggcaa atttatttt atatttgtt acatacctg 
      ttatttatt aaaaaatat aactattaa ccgactttg 
      tttactttt ttgaccaat ttacctaga caacataaa 
      aatagt