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Gene information
General information
TC009491 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid46: 230697..234203
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009491
    iBeetle screen iB_01562
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 10 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009491 RA
      MLASSRDKI TVRIQNMKR SSDRDTPPR SKRSRSSMG 
      RYDDSSDER LTPERVRRR GSRNRSPSP RPRYVSPHR 
      DEYLRPPER AYPYKVLCV SALHPKASD EVIKDTLYR 
      EYKKYGDLS IRLSHDVDE RVAYVCFRN PEDARDAKH 
      GKPRIIIYD KVAIVEPVY EVPRSSDIY RSRPRSITP 
      EYDRHYYAR SPERLRVDR YDRAYGPPP GVPVHRDYR 
      REALPAPHE YLARPPIHH PAHLTPSHG YGPPRHTVP 
      RPPFEKTEN KKDKFPNYL HHIQPEDDP LATRTLFAG 
      NLEINITEE ELRRIFGRY GVVEDIDIK RPPPGTGNA 
      FAFVRFHTL DMAHRAKVE LSGQYIGKF QCKIGYGKA 
      TPTTRIWVG GLGPWTSVP QLEREFDRF GAIKKIDYV 
      KGDNQAYIL YDSIDAAQA AVKEMRGAP LGGPDRRLR 
      TDFADVTPG VVYRPKPPY PGHEDSLDY RRLDYDYDY 
      DDYATRGYR GRAAYPERR AGSRGPYRY PETTEDEWG 
      RSRPEYERR RSVDRSRSR SPRRSPDSD SDNGSRRAG 
      LLASSRTLP EVARKCTTV WQGALILKN SLFPAKFHL 
      IDGDTDIVE GLMKDEDGK HQLRITQRL RLDQPKLED 
      VQRRISGAS SHAIFLGLA GSTASVTND DSTVQTRPL 
      RNLVSYLKQ KEAAGVISL LNKETEATG VLYSFPPCA 
      FSTELLKRT CPNLSEEAI KEDHLVIVV VRGGTA
    CDS sequence
    • >TC009491 RA
      atgctcgcc tccagccga gacaaaata acagtccgc 
      atccaaaac atgaaacgc agcagcgac cgcgacacg 
      cccccacgc agcaaacgc tcccgttca agcatgggc 
      cggtacgac gacagctcc gacgaacgc ctaaccccc 
      gaacgcgtc cgccgtcgt ggaagccgc aatcgctcg 
      cccagcccc cgccccagg tatgtcagc ccgcatcgc 
      gatgaatat ctgcgcccc cctgaacgc gcctaccct 
      tacaaagtg ctctgcgtc agcgcccta caccccaaa 
      gccagcgat gaagtgatc aaagacacg ctctatcga 
      gagtacaaa aaatacggc gatctgagc attagacta 
      tcgcatgat gttgatgaa cgcgtagct tatgtgtgc 
      ttccggaat ccggaggat gcacgtgat gccaaacac 
      gggaaaccc cggattatt atttatgat aaagttgcg 
      attgttgaa cctgtgtat gaagtgccc aggtcaagt 
      gatatttat aggagtcgc cccaggtct attacacca 
      gagtatgat cggcattat tatgctagg tcacctgaa 
      cggttaagg gttgatcgg tacgatagg gcttacggg 
      cccccgcca ggggtccca gtacataga gattatcgc 
      agggaggct ttacctgct cctcatgag tatttggca 
      aggcctccg attcatcac ccggcacat ttaacccca 
      agtcatgga tacggcccc ccacgacat acggtccca 
      aggcccccc tttgaaaaa acagagaat aaaaaagac 
      aagtttccg aattatttg caccacatt caaccagaa 
      gatgacccg ttagcgact cgtacgtta tttgccggt 
      aatttagaa attaatatc acagaagaa gagttgcgt 
      cgcattttc ggtcgttac ggtgttgtt gaagatata 
      gatatcaag agaccacca cctggcaca ggcaatgcc 
      tttgctttc gttcggttt cacaccctt gatatggcc 
      caccgtgct aaagtcgaa ttatcaggt caatacatc 
      ggaaagttc cagtgtaaa attggttac gggaaagca 
      accccaact acacgtatc tgggttggg gggcttggc 
      ccttggact tcagtcccc caactagaa cgtgagttt 
      gatcgtttt ggggctatc aagaaaatc gattacgtc 
      aagggcgac aatcaagcc tatattctg tacgacagt 
      atcgatgca gcacaagcg gctgtgaag gaaatgaga 
      ggagcccca cttgggggg cctgaccgt cgcctcagg 
      actgacttt gcggacgtg acaccggga gtcgtctac 
      cgacctaaa cccccctat cctggacat gaagacagt 
      ctcgattac cgccgcctt gactacgat tacgactac 
      gacgactac gccacccgg gggtacagg ggtcgtgct 
      gcctacccc gaacgccgc gccggcagc cggggccct 
      taccgctac cccgagaca actgaagat gaatggggg 
      cgctcacgc cccgaatac gaacgccgc cgttccgtt 
      gaccgctcc aggtccagg tcaccgaga cgttcccct 
      gattcggat tcggataat gggtctcgt agagctggg 
      ttgctcgca tctagtcgg actctcccc gaagtggca 
      cggaagtgt acgacagtg tggcaaggt gctttgatt 
      ttgaagaat tcgctgttt ccggccaag tttcatttg 
      attgatggc gatacggat attgtggaa gggttgatg 
      aaggacgag gatggaaaa catcagctg aggattacg 
      caaaggttg aggttggat cagccaaag ttggaggat 
      gtgcaaagg agaatttcg ggggcgagt tcgcatgcg 
      attttcttg gggttggcg gggtcgacg gcgtcggtc 
      acgaatgat gatagtacg gtgcagacg aggccgttg 
      aggaatttg gtgtcgtat ttgaagcag aaggaagca 
      gctggggtt atttcgttg ttgaataag gagacggaa 
      gcgacaggg gttttgtat tcgtttccg ccgtgtgcg 
      ttttcgaca gagttgctt aaacgtacg tgtccgaat 
      ctgtcggag gaggcgatc aaggaggat catttggtt 
      attgttgtg gtacgtggt ggtactgct taa
    mRNA sequence
    • >TC009491 RA
      tttgccttg tcacatccg ccatattgt ccaccattc 
      gcttccatt tctgtgatg ccccaacgc agcactcat 
      ccacctcag taaatggtg ataaaactc attattgtg 
      cccctaagc gttagtgtt tatgctcgc ctccagccg 
      agacaaaat aacagtccg catccaaaa catgaaacg 
      cagcagcga ccgcgacac gcccccacg cagcaaacg 
      ctcccgttc aagcatggg ccggtacga cgacagctc 
      cgacgaacg cctaacccc cgaacgcgt ccgccgtcg 
      tggaagccg caatcgctc gcccagccc ccgccccag 
      gtatgtcag cccgcatcg cgatgaata tctgcgccc 
      ccctgaacg cgcctaccc ttacaaagt gctctgcgt 
      cagcgccct acaccccaa agccagcga tgaagtgat 
      caaagacac gctctatcg agagtacaa aaaatacgg 
      cgatctgag cattagact atcgcatga tgttgatga 
      acgcgtagc ttatgtgtg cttccggaa tccggagga 
      tgcacgtga tgccaaaca cgggaaacc ccggattat 
      tatttatga taaagttgc gattgttga acctgtgta 
      tgaagtgcc caggtcaag tgatattta taggagtcg 
      ccccaggtc tattacacc agagtatga tcggcatta 
      ttatgctag gtcacctga acggttaag ggttgatcg 
      gtacgatag ggcttacgg gcccccgcc aggggtccc 
      agtacatag agattatcg cagggaggc tttacctgc 
      tcctcatga gtatttggc aaggcctcc gattcatca 
      cccggcaca tttaacccc aagtcatgg atacggccc 
      cccacgaca tacggtccc aaggccccc ctttgaaaa 
      aacagagaa taaaaaaga caagtttcc gaattattt 
      gcaccacat tcaaccaga agatgaccc gttagcgac 
      tcgtacgtt atttgccgg taatttaga aattaatat 
      cacagaaga agagttgcg tcgcatttt cggtcgtta 
      cggtgttgt tgaagatat agatatcaa gagaccacc 
      acctggcac aggcaatgc ctttgcttt cgttcggtt 
      tcacaccct tgatatggc ccaccgtgc taaagtcga 
      attatcagg tcaatacat cggaaagtt ccagtgtaa 
      aattggtta cgggaaagc aaccccaac tacacgtat 
      ctgggttgg ggggcttgg cccttggac ttcagtccc 
      ccaactaga acgtgagtt tgatcgttt tggggctat 
      caagaaaat cgattacgt caagggcga caatcaagc 
      ctatattct gtacgacag tatcgatgc agcacaagc 
      ggctgtgaa ggaaatgag aggagcccc acttggggg 
      gcctgaccg tcgcctcag gactgactt tgcggacgt 
      gacaccggg agtcgtcta ccgacctaa accccccta 
      tcctggaca tgaagacag tctcgatta ccgccgcct 
      tgactacga ttacgacta cgacgacta cgccacccg 
      ggggtacag gggtcgtgc tgcctaccc cgaacgccg 
      cgccggcag ccggggccc ttaccgcta ccccgagac 
      aactgaaga tgaatgggg gcgctcacg ccccgaata 
      cgaacgccg ccgttccgt tgaccgctc caggtccag 
      gtcaccgag acgttcccc tgattcgga ttcggataa 
      tgggtctcg tagagctgg gttgctcgc atctagtcg 
      gactctccc cgaagtggc acggaagtg tacgacagt 
      gtggcaagg tgctttgat tttgaagaa ttcgctgtt 
      tccggccaa gtttcattt gattgatgg cgatacgga 
      tattgtgga agggttgat gaaggacga ggatggaaa 
      acatcagct gaggattac gcaaaggtt gaggttgga 
      tcagccaaa gttggagga tgtgcaaag gagaatttc 
      gggggcgag ttcgcatgc gattttctt ggggttggc 
      ggggtcgac ggcgtcggt cacgaatga tgatagtac 
      ggtgcagac gaggccgtt gaggaattt ggtgtcgta 
      tttgaagca gaaggaagc agctggggt tatttcgtt 
      gttgaataa ggagacgga agcgacagg ggttttgta 
      ttcgtttcc gccgtgtgc gttttcgac agagttgct 
      taaacgtac gtgtccgaa tctgtcgga ggaggcgat 
      caaggagga tcatttggt tattgttgt ggtacgtgg 
      tggtactgc ttaattttt atgactgta tagtcgttg 
      actatactg tattattat tttgttttt ttttactta 
      ttttatgag gtagagctc atttttttt acccccctc 
      caggtctac actggctaa ttttgttga cagtttaaa 
      caaattgta catttttca ggaaattgg tgatcaaca 
      acgatgttg attgtgttg atgatgatg atgatgctt 
      tatcattta agtgcttac tgaatttgt tttgtgaac 
      tttgaaaat attctgtaa cttgattta gtgctgaag 
      tgaatttag tttaagtgg ttgcagttc tttctcata 
      gtatctgtc atttgtatt agtggaaca ttctgtaag 
      gatttatct gttgttatg tgaaaggac tgtaaaagg 
      aaacaatta actacagtg ttgtagtgt tctgtgcaa 
      atagttact ctttataaa gattgtttc aaaataaaa 
      ccaagttaa tgattgtga tgtagtgtt aagacactt 
      atcagtggc taggaagta tggtcttct ataatcatt 
      tctcctgtc tggttataa aatgttcaa tagtgttgt 
      gcaaataga taagttctt tcagacgat tgtgttgtt 
      ctgttcttc aaatattcc ttgtgagtg tgaatgtgc 
      acaaagtcc aaatttcgc gcggagttt tcttttcgt 
      agtagtgga aaaacgtaa tttaatggc tgtgatttt 
      ctctaattg gatttctct cattttatt tggaattaa 
      acgagttca gtgattcga atttagtga gcaaaattt 
      ttcgtgttg ttgaacatt gtgagaaca ctaaatttt 
      tgtgaatag tgttttcaa catattttt aaacttgta 
      cacagtcat attcgttaa taaaaactc taagttgga 
      taaatcatt ctaccttca gcaaatagc caattggta 
      agttcctcc agctttact aagtaattt agcaaccca 
      taagcggga ctcataatt tcagtaaaa gaagttttt 
      ttttaattt aaactaact tataaagta acagtttac 
      aattttaag tacctagaa cagtctaca ccatattga 
      gggtagtat ttgtagtca gggtaagta tttctttaa 
      tgttaaaag attaaattg atttttcaa tagatattt 
      tgtatgcac ttcaattaa taaaatgat ggttaagaa