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Gene information
General information
TC009486 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 414807..417805
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009486
    iBeetle screen iB_01561

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009486 RA
      MLFKSVIFS CFLALVYGD CSNKAPAWS NTYTVKGIL 
      YIPYAEIEE PFYAWYDGQ SKRSRIDYY GDMVKTYQL 
      STEGPYGSS LKIAPVTTE TETNSRTCL QVNGTEENT 
      IEPQSILPD LTGFECTGE ETISGVLTE KWALSQTIG 
      QKVNKYAMW LYYKDDPDN TGVKIAVPV KYEMRGFNS 
      LLGSHYDHY YLDYDSFSS DPIPDDIFK FDSNMKCSA 
      FPGPGDKHI YTFNPMAEF IRPEKSGHV DFEFGKFTR 
      KHGKNYQNK TETLMRKDI FRQNVRFIH SMNRQNRGF 
      TLTVNHLAD KTPTELKAL RGRTYSGGY NGGAPFPYE 
      NINKEDLPD QWDWRLLGA VTPVKDQSV CGSCWSFGT 
      VGTVEGALF LHNGGRLFR LSQQALVDC SWGYGNNGC 
      DGGEDFRAY QWMLKHGGI PTEEAYGPY LGQDGYCHA 
      DKVQKVAKI TGYVNVTTN DENALRLAL FKHGPISVA 
      IDASQRTFS FYSNGVYYE PKCGNKIDE LDHAVLAVG 
      YGTINGENY WLVKNSWSN YWGNDGYIL MSSKDNNCG 
      VMTTPTYVT M
    CDS sequence
    • >TC009486 RA
      atgttattt aaaagtgtg attttttcg tgttttttg 
      gcactagtc tatggggac tgttccaac aaagcgccg 
      gcttggagc aacacatac acggtcaag ggcattctt 
      tacatccct tatgctgaa attgaggaa cctttctac 
      gcctggtac gatggccaa tcgaaacgc tcccgtatt 
      gactactac ggcgatatg gtgaaaacc taccaactc 
      tccacagag gggccatac ggcagcagc cttaaaata 
      gcccctgtg acaacagag accgaaacc aactcccga 
      acctgtctc caagtcaac ggaactgaa gaaaacaca 
      attgagccc caatccatt ctccctgac ctcacaggt 
      tttgaatgt actggagag gagacaatt agcggagtc 
      ctaaccgaa aaatgggcc ctgtctcaa acaatcggc 
      caaaaagtc aacaaatac gccatgtgg ctgtattac 
      aaagacgac ccggacaac accggagta aaaatcgcc 
      gttccggtc aaatacgaa atgaggggc tttaattca 
      cttttgggg agtcactat gaccactac tacctcgat 
      tatgactcc ttcagctca gaccccatc ccggacgac 
      atcttcaaa tttgactca aacatgaaa tgtagtgcc 
      ttcccgggc cctggcgac aaacacatt tacactttt 
      aacccaatg gctgaattc atcagacct gaaaaaagt 
      ggccatgtt gatttcgaa tttggcaaa tttacgcga 
      aaacatggc aaaaattac caaaataaa accgaaact 
      ctcatgcgt aaagatatt ttccgccaa aacgtccga 
      tttatccat tcgatgaac agacaaaat agggggttc 
      accctgact gtgaatcac ttggctgat aaaacccca 
      accgaattg aaggcgttg cgagggcgc acttattcg 
      gggggctac aacggtggg gcccccttc ccgtatgaa 
      aacataaat aaggaggat ttgcccgat cagtgggac 
      tggaggctg ttgggggcc gtaacacct gttaaagat 
      caatcggtt tgtggttcg tgttggagt ttcggaact 
      gtgggtaca gtcgagggg gcacttttt ttgcacaat 
      gggggccga cttttccgg ttgtctcaa caagctcta 
      gttgattgt tcttggggg tatggaaat aacggttgt 
      gatgggggt gaggatttc agggcatat caatggatg 
      ctcaaacat ggtggtatc cccactgag gaggcctat 
      ggcccctat ttgggacaa gacggttac tgtcatgca 
      gataaggtg caaaaagtt gccaaaatc accggttat 
      gttaacgtt acaacaaat gatgaaaat gccttgagg 
      ttggctttg ttcaaacat gggcctatt agtgtggcc 
      attgatgct agtcaacgc actttcagt ttttactcc 
      aatggggtt tattacgaa cccaaatgt ggtaataaa 
      attgacgaa ttagatcat gccgttctg gcagttggg 
      tatggaacc ataaatggt gaaaattac tggttggtt 
      aaaaacagt tggtcgaat tattggggc aacgatggc 
      tacatccta atgtcgtcc aaagacaac aattgcgga 
      gtcatgaca actccaacc tacgtaaca atgtaa
    mRNA sequence
    • >TC009486 RA
      ataggtcgt gggggttgt ttttaaaag gaggcctct 
      tatcggcta tttgtttat ttggagatt tgacgccaa 
      atgagaatg ttatttaaa agtgtgatt ttttcgtgt 
      tttttggca ctagtctat ggggactgt tccaacaaa 
      gcgccggct tggagcaac acatacacg gtcaagggc 
      attctttac atcccttat gctgaaatt gaggaacct 
      ttctacgcc tggtacgat ggccaatcg aaacgctcc 
      cgtattgac tactacggc gatatggtg aaaacctac 
      caactctcc acagagggg ccatacggc agcagcctt 
      aaaatagcc cctgtgaca acagagacc gaaaccaac 
      tcccgaacc tgtctccaa gtcaacgga actgaagaa 
      aacacaatt gagccccaa tccattctc cctgacctc 
      acaggtttt gaatgtact ggagaggag acaattagc 
      ggagtccta accgaaaaa tgggccctg tctcaaaca 
      atcggccaa aaagtcaac aaatacgcc atgtggctg 
      tattacaaa gacgacccg gacaacacc ggagtaaaa 
      atcgccgtt ccggtcaaa tacgaaatg aggggcttt 
      aattcactt ttggggagt cactatgac cactactac 
      ctcgattat gactccttc agctcagac cccatcccg 
      gacgacatc ttcaaattt gactcaaac atgaaatgt 
      agtgccttc ccgggccct ggcgacaaa cacatttac 
      acttttaac ccaatggct gaattcatc agacctgaa 
      aaaagtggc catgttgat ttcgaattt ggcaaattt 
      acgcgaaaa catggcaaa aattaccaa aataaaacc 
      gaaactctc atgcgtaaa gatattttc cgccaaaac 
      gtccgattt atccattcg atgaacaga caaaatagg 
      gggttcacc ctgactgtg aatcacttg gctgataaa 
      accccaacc gaattgaag gcgttgcga gggcgcact 
      tattcgggg ggctacaac ggtggggcc cccttcccg 
      tatgaaaac ataaataag gaggatttg cccgatcag 
      tgggactgg aggctgttg ggggccgta acacctgtt 
      aaagatcaa tcggtttgt ggttcgtgt tggagtttc 
      ggaactgtg ggtacagtc gagggggca ctttttttg 
      cacaatggg ggccgactt ttccggttg tctcaacaa 
      gctctagtt gattgttct tgggggtat ggaaataac 
      ggttgtgat gggggtgag gatttcagg gcatatcaa 
      tggatgctc aaacatggt ggtatcccc actgaggag 
      gcctatggc ccctatttg ggacaagac ggttactgt 
      catgcagat aaggtgcaa aaagttgcc aaaatcacc 
      ggttatgtt aacgttaca acaaatgat gaaaatgcc 
      ttgaggttg gctttgttc aaacatggg cctattagt 
      gtggccatt gatgctagt caacgcact ttcagtttt 
      tactccaat ggggtttat tacgaaccc aaatgtggt 
      aataaaatt gacgaatta gatcatgcc gttctggca 
      gttgggtat ggaaccata aatggtgaa aattactgg 
      ttggttaaa aacagttgg tcgaattat tggggcaac 
      gatggctac atcctaatg tcgtccaaa gacaacaat 
      tgcggagtc atgacaact ccaacctac gtaacaatg 
      taataaaat tgtgactga gtcttattt ggtttaata 
      aataatggt tacttta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY