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Gene information
General information
TC009472 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid46: 482534..484912
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009472
    iBeetle screen iB_01560
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt precocious - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection



    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection



    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection



    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009472 RA
      MGRRKPGFK KNHQFSNQK QGGQSNVDT RKAYNDIVK 
      ENEKFVKYY EVQNVCKKD EYDAFITSL KTDLPATFR 
      ITGSKGEAQ KMLEIVQGQ FIKDCLSQG DSEGKPPNI 
      FPLPWYPNK LAWQLELTR KDIRRCEAY YKLHNFLIS 
      ETEHGTISR QETVSMIPP LLLDVQPHH KVLDMCAAP 
      GSKTAQLLE MLHSNDDPI PSGYVIAND VDNKRCYML 
      VHQAKRLNS PCVAVINHD SAILPNLSA SLPDGSMEQ 
      VQFDRVLCD VPCSGDGTL RKNPDIWMK WTPANGLNL 
      HGIQSRILR RGAELLTVG GRLVYSTCS INPIENEAV 
      IHRLLSETD GALQLVDVS SSLPGLKYM PGLENWLVG 
      SRNLEFYSS YDEVDEKWQ TTIRPQMFP PKSEDKHLY 
      NLNRCMRIL PHHQNTGAF FVAVLEKLK PLNSKEKSF 
      KTNEIPETN SNNKRENDD LPQNQRKKR RKEGYREDP 
      FVFFKEEES VWNEIKSFY EISDDFDSK CLLTRCHVG 
      KKKNIYLTS NAVRDLVVQ NQNVIKFIN TGVKAFVRC 
      DNKNMKCAF RIANDGLES IYPYIGDGR KIDIPREDL 
      ITLLLNDNP EKSPPIASL SEIVQKQVE HLSKFYLIM 
      KK
    CDS sequence
    • >TC009472 RA
      atggggcgc cggaagccg ggttttaag aaaaaccac 
      cagttttcc aaccagaaa caagggggc caaagtaat 
      gtggacact cgcaaggct tacaacgac atagtaaaa 
      gaaaacgaa aagtttgtc aagtattac gaagtgcaa 
      aacgtgtgc aagaaagat gagtatgat gcgtttatc 
      actagttta aagacggac ttgccggcg actttccgc 
      atcactggc tcgaaggga gaggcccaa aaaatgctc 
      gaaatcgtc caaggccag tttattaag gattgctta 
      agtcagggt gatagtgag gggaagccc ccaaacata 
      ttccctctc ccttggtac cccaataaa ttagcctgg 
      cagttggaa ctgacacgt aaagacata agacgctgt 
      gaggcctat tacaaactc cacaatttc ctaatttcg 
      gaaacggaa catgggaca atttcacgc caggaaacc 
      gtcagtatg atacctccc ttattactc gacgtgcaa 
      ccccatcac aaagttcta gatatgtgt gccgccccc 
      ggctccaaa accgcccaa cttttggag atgttgcac 
      tccaatgat gacccgatt ccttccggt tatgttatc 
      gcaaatgac gttgataac aagcggtgt tatatgttg 
      gtgcaccaa gctaaaagg ctgaattcg ccgtgtgtg 
      gctgtaatc aaccatgat agcgcaatt ttacccaac 
      ctatcggcg tctctgcct gatgggtca atggaacaa 
      gtccagttt gatcgggtg ctgtgcgat gtaccatgt 
      tctggagat gggactttg cgcaaaaat ccagacatt 
      tggatgaag tggacgccc gcaaacggg ttgaatttg 
      cacggaatt cagtctaga attttgcga agaggggcc 
      gaattatta acagtaggc ggccgattg gtgtactca 
      acttgttca ataaaccca attgagaac gaagctgta 
      attcataga ttattatca gagactgat ggagcgctt 
      caactagtt gatgttagt agttcgtta cctggactt 
      aaatatatg ccaggtttg gagaattgg ttagttggg 
      agccggaac ttggaattt tattcatct tatgatgaa 
      gtggacgag aaatggcag acgacaatc aggccacaa 
      atgtttccg ccaaaatct gaagacaaa catttgtat 
      aatttaaac agatgcatg cgtatttta ccccaccac 
      caaaacacc ggtgctttc ttcgttgca gtgttagaa 
      aaactcaaa cctctcaac tcgaaagaa aaatcattt 
      aaaacgaac gaaatcccc gaaactaac agtaacaac 
      aaacgcgaa aatgacgat ttgccacaa aatcagcga 
      aaaaaacgc cggaaagaa ggttacaga gaggatccg 
      ttcgttttt ttcaaagaa gaggaatcg gtttggaac 
      gaaattaaa tcattttat gaaatttcc gacgatttc 
      gattcgaaa tgtctcttg actcgatgt catgtcggt 
      aaaaaaaag aacatttat ttgacgtcg aatgcagtc 
      cgcgacttg gttgtacaa aatcagaac gtaatcaag 
      tttattaat acgggtgtt aaggcgttt gttcgctgt 
      gacaataaa aacatgaaa tgtgccttc aggattgcc 
      aatgatggg cttgaaagt atttatccc tacattgga 
      gatgggaga aagatagac attccgaga gaagattta 
      attactttg cttttgaac gacaatcca gagaagtcg 
      cctccgatt gcttcattg tcagaaatt gtacagaag 
      caagttgaa catttaagt aagttttac ttgattatg 
      aaaaaataa
    mRNA sequence
    • >TC009472 RA
      taggtctgg tgaccacgt ggttgtttt ttcaggtta 
      gaaaaaact ggctgtaat ttgtaccac cacacgaat 
      attgtgcaa aatggggcg ccggaagcc gggttttaa 
      gaaaaacca ccagttttc caaccagaa acaaggggg 
      ccaaagtaa tgtggacac tcgcaaggc ttacaacga 
      catagtaaa agaaaacga aaagtttgt caagtatta 
      cgaagtgca aaacgtgtg caagaaaga tgagtatga 
      tgcgtttat cactagttt aaagacgga cttgccggc 
      gactttccg catcactgg ctcgaaggg agaggccca 
      aaaaatgct cgaaatcgt ccaaggcca gtttattaa 
      ggattgctt aagtcaggg tgatagtga ggggaagcc 
      cccaaacat attccctct cccttggta ccccaataa 
      attagcctg gcagttgga actgacacg taaagacat 
      aagacgctg tgaggccta ttacaaact ccacaattt 
      cctaatttc ggaaacgga acatgggac aatttcacg 
      ccaggaaac cgtcagtat gatacctcc cttattact 
      cgacgtgca accccatca caaagttct agatatgtg 
      tgccgcccc cggctccaa aaccgccca acttttgga 
      gatgttgca ctccaatga tgacccgat tccttccgg 
      ttatgttat cgcaaatga cgttgataa caagcggtg 
      ttatatgtt ggtgcacca agctaaaag gctgaattc 
      gccgtgtgt ggctgtaat caaccatga tagcgcaat 
      tttacccaa cctatcggc gtctctgcc tgatgggtc 
      aatggaaca agtccagtt tgatcgggt gctgtgcga 
      tgtaccatg ttctggaga tgggacttt gcgcaaaaa 
      tccagacat ttggatgaa gtggacgcc cgcaaacgg 
      gttgaattt gcacggaat tcagtctag aattttgcg 
      aagaggggc cgaattatt aacagtagg cggccgatt 
      ggtgtactc aacttgttc aataaaccc aattgagaa 
      cgaagctgt aattcatag attattatc agagactga 
      tggagcgct tcaactagt tgatgttag tagttcgtt 
      acctggact taaatatat gccaggttt ggagaattg 
      gttagttgg gagccggaa cttggaatt ttattcatc 
      ttatgatga agtggacga gaaatggca gacgacaat 
      caggccaca aatgtttcc gccaaaatc tgaagacaa 
      acatttgta taatttaaa cagatgcat gcgtatttt 
      accccacca ccaaaacac cggtgcttt cttcgttgc 
      agtgttaga aaaactcaa acctctcaa ctcgaaaga 
      aaaatcatt taaaacgaa cgaaatccc cgaaactaa 
      cagtaacaa caaacgcga aaatgacga tttgccaca 
      aaatcagcg aaaaaaacg ccggaaaga aggttacag 
      agaggatcc gttcgtttt tttcaaaga agaggaatc 
      ggtttggaa cgaaattaa atcatttta tgaaatttc 
      cgacgattt cgattcgaa atgtctctt gactcgatg 
      tcatgtcgg taaaaaaaa gaacattta tttgacgtc 
      gaatgcagt ccgcgactt ggttgtaca aaatcagaa 
      cgtaatcaa gtttattaa tacgggtgt taaggcgtt 
      tgttcgctg tgacaataa aaacatgaa atgtgcctt 
      caggattgc caatgatgg gcttgaaag tatttatcc 
      ctacattgg agatgggag aaagataga cattccgag 
      agaagattt aattacttt gcttttgaa cgacaatcc 
      agagaagtc gcctccgat tgcttcatt gtcagaaat 
      tgtacagaa gcaagttga acatttaag taagtttta 
      cttgattat gaaaaaata ataacgtca ataatgatc 
      ttggctacc t