General information | |||
TC009450 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 1012160..1017444 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC009450 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01556 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
gena triplet gena triplet posterior not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
antenna sometimes not present - Penetrance (percentage of animals/eggs on slide): less than 30%
head shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
legs shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
tropharium size decreased - Number of animals showing the phenotype: 3
mature egg not present - Number of animals showing the phenotype: 3
vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 3
previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 3
- >TC009450 RA
MSDYSAVAP PPQQNFGPS SAFAAALQR AKQIAAKIN PGSTNPADN RPKRPLEDG PEPDAKKGP STSGPPPQL PPPGNRPPP SQGGMGGPP MGGGPQLNE DIKVPDKMV GLIIGRGGE QITRLQSES GCKIQMAPD SQGMPDRVC SLSGTKEAI NRAKELIMN IVHQRGRTE GLGGLDLGP NMGHNNYPG MNNMNQGMG GGGGGGGGG GGGRNFVEI MIPGPKVGL IIGKGGETI KQLQEKSGA KMVVIQDGP NQEQEKPLR ISGDPSKVE YAKQLVYDL IAEKEMQNY NRRGGGGRQ DDRQQYNDY GGGGGNEAE VLVPRQAVG VVIGKGGDM IKKIQAETG ARVQFQQAR EEGPGERRC YLSGTPKQV EQARQRIEE LIDSVHRRD GGDGPGQGR GRGRGGDRY DSRGGSNRN GGGQDYGGW DDRRQQQAP QEVTFVVPS SKCGVIIGR GGETIKQIN QQSGAHCEL DRRSQNNQN SNEKTFIIR GDPDQIEAA KRIISDKVQ MPLNFVPVG GPAMSNNMQ TAYPGMAPQ SYNPQNWGM PAYQQQWNA PSQSADAPT QQNAMQVNP STGQPDYSL QWAEYYRSL GMHREAEMI EQQAKSKGM SSSAPGQPQ AGATAGSGP APAATPTGA ASNGGQPDY SAQWAEYYR SIGKHKEAE AIEAQMKSK QQSQANVQS MQGGPASSQ SGPVQAPAG GYQQQAYGA YQASSGYYG GQAQGGVPS NVPQGGYGF PAYGYGAPA PAAPPNNSD N
- >TC009450 RA
atgagtgat tattcagcg gtagcgccg ccacctcaa cagaatttt ggtccttcc tcggctttt gcggccgcc ctccaaaga gcaaaacaa atcgctgct aaaattaac ccaggatcc acaaacccg gccgataat cgaccgaaa aggcctcta gaagacggg ccagaaccg gatgcgaag aaaggtcca agcaccagt ggaccacct cctcaactg cctccgcct ggaaacaga ccgccgccg agccagggt ggcatgggt ggaccccca atgggtggc gggccgcag cttaatgaa gacatcaaa gtgcctgat aaaatggtt ggcttaata attggtcgt ggaggcgaa cagatcacg agacttcag tcggaatca ggctgtaaa atccaaatg gcacctgat tcacaggga atgccagac cgtgtatgt tcgctatca ggcactaaa gaggccatc aatagagcc aaggagctc attatgaat atcgttcat caaagaggt cgcacggaa gggttgggt gggctcgat ctagggcca aatatgggt cacaataat tacccaggg atgaacaac atgaaccag gggatgggt ggtggtggc ggcggcggc ggtggcggt ggcggcggc cgtaacttc gtcgaaatc atgatacct ggacccaaa gtcggtctg ataatcggt aaaggtggc gaaaccatc aaacaactg caggaaaaa agcggcgcg aaaatggtt gtgattcaa gacggtcct aatcaagaa caagaaaaa ccgttacgg atcagtggt gacccttcc aaagtcgaa tacgccaaa caattagtc tacgatttg attgccgag aaggagatg caaaattat aatcgaaga ggtggaggc gggagacaa gacgatagg caacagtac aacgattat ggagggggt ggtggtaat gaggcggag gttttagtg ccgagacag gctgtgggg gttgttatt ggaaaaggg ggcgatatg attaagaaa attcaagcg gaaactggg gcgcgggtt cagttccag caagccaga gaggagggg ccgggggag aggcggtgc tacctctca ggcaccccc aaacaagtc gaacaggct agacaacga atagaggaa cttatcgac agcgtccat cgacgagac ggcggcgac ggtcccggc caaggccgt ggcagagga agaggtggc gatcgttac gacagccga ggtggaagt aacagaaac ggcggagga caagactac ggcggttgg gacgacaga agacaacaa caagcacct caagaagtc acattcgtt gttccaagt tcgaaatgt ggagtaata atcgggaga ggtggcgaa acgatcaaa cagatcaat caacagtcc ggtgcccac tgtgagtta gaccgccga tcacaaaac aatcagaat tcaaacgaa aaaacattt ataatccgc ggcgatcct gatcaaatc gaagcggcc aaacgaata atcagcgat aaagtccaa atgcctctt aacttcgta cctgttggc ggtcctgca atgtcaaac aacatgcaa actgcttat cctggaatg gcgcctcaa tcttacaac ccccaaaat tggggcatg cctgcttac caacaacaa tggaacgcc ccctcgcaa agtgccgac gcccccaca caacaaaac gccatgcag gttaacccc tcaactgga cagcctgac tactcgcta cagtgggca gagtattac aggtcctta ggaatgcac agggaagcg gaaatgata gaacaacag gcaaaatcg aagggaatg tcctcgtcg gcccccggc caaccccaa gccggtgcg acggcaggt tccggtcca gcacctgca gcaaccccc actggtgct gcatccaat ggtggccaa ccagattac agtgcccag tgggcggag tattatcgt agtatagga aaacataaa gaagctgaa gcgatagaa gcacaaatg aaatcgaag caacagtcc caagccaac gtgcaatcg atgcaagga ggcccagct tcgtcccag tccggccct gtccaagcc ccagcaggt ggttaccag cagcaagcc tatggtgca tatcaagct tcaagtggt tactacggt ggacaggcg caaggtggg gtccccagc aacgtcccc caagggggc tacggcttc ccggcgtac ggttacgga gcacccgcc cctgcagcc ccccctaac aactccgac aactaa
- >TC009450 RA
cacgtttgt acgcgggcg actgggcga caccatttt ctgttttct atgtggaca aaattttat taatggtga taaaaaatg agtgattat tcagcggta gcgccgcca cctcaacag aattttggt ccttcctcg gcttttgcg gccgccctc caaagagca aaacaaatc gctgctaaa attaaccca ggatccaca aacccggcc gataatcga ccgaaaagg cctctagaa gacgggcca gaaccggat gcgaagaaa ggtccaagc accagtgga ccacctcct caactgcct ccgcctgga aacagaccg ccgccgagc cagggtggc atgggtgga cccccaatg ggtggcggg ccgcagctt aatgaagac atcaaagtg cctgataaa atggttggc ttaataatt ggtcgtgga ggcgaacag atcacgaga cttcagtcg gaatcaggc tgtaaaatc caaatggca cctgattca cagggaatg ccagaccgt gtatgttcg ctatcaggc actaaagag gccatcaat agagccaag gagctcatt atgaatatc gttcatcaa agaggtcgc acggaaggg ttgggtggg ctcgatcta gggccaaat atgggtcac aataattac ccagggatg aacaacatg aaccagggg atgggtggt ggtggcggc ggcggcggt ggcggtggc ggcggccgt aacttcgtc gaaatcatg atacctgga cccaaagtc ggtctgata atcggtaaa ggtggcgaa accatcaaa caactgcag gaaaaaagc ggcgcgaaa atggttgtg attcaagac ggtcctaat caagaacaa gaaaaaccg ttacggatc agtggtgac ccttccaaa gtcgaatac gccaaacaa ttagtctac gatttgatt gccgagaag gagatgcaa aattataat cgaagaggt ggaggcggg agacaagac gataggcaa cagtacaac gattatgga gggggtggt ggtaatgag gcggaggtt ttagtgccg agacaggct gtgggggtt gttattgga aaagggggc gatatgatt aagaaaatt caagcggaa actggggcg cgggttcag ttccagcaa gccagagag gaggggccg ggggagagg cggtgctac ctctcaggc acccccaaa caagtcgaa caggctaga caacgaata gaggaactt atcgacagc gtccatcga cgagacggc ggcgacggt cccggccaa ggccgtggc agaggaaga ggtggcgat cgttacgac agccgaggt ggaagtaac agaaacggc ggaggacaa gactacggc ggttgggac gacagaaga caacaacaa gcacctcaa gaagtcaca ttcgttgtt ccaagttcg aaatgtgga gtaataatc gggagaggt ggcgaaacg atcaaacag atcaatcaa cagtccggt gcccactgt gagttagac cgccgatca caaaacaat cagaattca aacgaaaaa acatttata atccgcggc gatcctgat caaatcgaa gcggccaaa cgaataatc agcgataaa gtccaaatg cctcttaac ttcgtacct gttggcggt cctgcaatg tcaaacaac atgcaaact gcttatcct ggaatggcg cctcaatct tacaacccc caaaattgg ggcatgcct gcttaccaa caacaatgg aacgccccc tcgcaaagt gccgacgcc cccacacaa caaaacgcc atgcaggtt aacccctca actggacag cctgactac tcgctacag tgggcagag tattacagg tccttagga atgcacagg gaagcggaa atgatagaa caacaggca aaatcgaag ggaatgtcc tcgtcggcc cccggccaa ccccaagcc ggtgcgacg gcaggttcc ggtccagca cctgcagca acccccact ggtgctgca tccaatggt ggccaacca gattacagt gcccagtgg gcggagtat tatcgtagt ataggaaaa cataaagaa gctgaagcg atagaagca caaatgaaa tcgaagcaa cagtcccaa gccaacgtg caatcgatg caaggaggc ccagcttcg tcccagtcc ggccctgtc caagcccca gcaggtggt taccagcag caagcctat ggtgcatat caagcttca agtggttac tacggtgga caggcgcaa ggtggggtc cccagcaac gtcccccaa gggggctac ggcttcccg gcgtacggt tacggagca cccgcccct gcagccccc cctaacaac tccgacaac taagactct acgtgtaag taaaaattt tgcttgtat ataatacgg acgtgtcgc ttcctta
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
