Status
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Gene information
General information
TC009450 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1012160..1017444
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009450
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01556

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): more than 80%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    gena triplet gena triplet posterior not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    antenna sometimes not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    legs shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    tropharium size decreased - Number of animals showing the phenotype: 3

    mature egg not present - Number of animals showing the phenotype: 3
    vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 3
    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009450 RA
      MSDYSAVAP PPQQNFGPS SAFAAALQR AKQIAAKIN 
      PGSTNPADN RPKRPLEDG PEPDAKKGP STSGPPPQL 
      PPPGNRPPP SQGGMGGPP MGGGPQLNE DIKVPDKMV 
      GLIIGRGGE QITRLQSES GCKIQMAPD SQGMPDRVC 
      SLSGTKEAI NRAKELIMN IVHQRGRTE GLGGLDLGP 
      NMGHNNYPG MNNMNQGMG GGGGGGGGG GGGRNFVEI 
      MIPGPKVGL IIGKGGETI KQLQEKSGA KMVVIQDGP 
      NQEQEKPLR ISGDPSKVE YAKQLVYDL IAEKEMQNY 
      NRRGGGGRQ DDRQQYNDY GGGGGNEAE VLVPRQAVG 
      VVIGKGGDM IKKIQAETG ARVQFQQAR EEGPGERRC 
      YLSGTPKQV EQARQRIEE LIDSVHRRD GGDGPGQGR 
      GRGRGGDRY DSRGGSNRN GGGQDYGGW DDRRQQQAP 
      QEVTFVVPS SKCGVIIGR GGETIKQIN QQSGAHCEL 
      DRRSQNNQN SNEKTFIIR GDPDQIEAA KRIISDKVQ 
      MPLNFVPVG GPAMSNNMQ TAYPGMAPQ SYNPQNWGM 
      PAYQQQWNA PSQSADAPT QQNAMQVNP STGQPDYSL 
      QWAEYYRSL GMHREAEMI EQQAKSKGM SSSAPGQPQ 
      AGATAGSGP APAATPTGA ASNGGQPDY SAQWAEYYR 
      SIGKHKEAE AIEAQMKSK QQSQANVQS MQGGPASSQ 
      SGPVQAPAG GYQQQAYGA YQASSGYYG GQAQGGVPS 
      NVPQGGYGF PAYGYGAPA PAAPPNNSD N
    CDS sequence
    • >TC009450 RA
      atgagtgat tattcagcg gtagcgccg ccacctcaa 
      cagaatttt ggtccttcc tcggctttt gcggccgcc 
      ctccaaaga gcaaaacaa atcgctgct aaaattaac 
      ccaggatcc acaaacccg gccgataat cgaccgaaa 
      aggcctcta gaagacggg ccagaaccg gatgcgaag 
      aaaggtcca agcaccagt ggaccacct cctcaactg 
      cctccgcct ggaaacaga ccgccgccg agccagggt 
      ggcatgggt ggaccccca atgggtggc gggccgcag 
      cttaatgaa gacatcaaa gtgcctgat aaaatggtt 
      ggcttaata attggtcgt ggaggcgaa cagatcacg 
      agacttcag tcggaatca ggctgtaaa atccaaatg 
      gcacctgat tcacaggga atgccagac cgtgtatgt 
      tcgctatca ggcactaaa gaggccatc aatagagcc 
      aaggagctc attatgaat atcgttcat caaagaggt 
      cgcacggaa gggttgggt gggctcgat ctagggcca 
      aatatgggt cacaataat tacccaggg atgaacaac 
      atgaaccag gggatgggt ggtggtggc ggcggcggc 
      ggtggcggt ggcggcggc cgtaacttc gtcgaaatc 
      atgatacct ggacccaaa gtcggtctg ataatcggt 
      aaaggtggc gaaaccatc aaacaactg caggaaaaa 
      agcggcgcg aaaatggtt gtgattcaa gacggtcct 
      aatcaagaa caagaaaaa ccgttacgg atcagtggt 
      gacccttcc aaagtcgaa tacgccaaa caattagtc 
      tacgatttg attgccgag aaggagatg caaaattat 
      aatcgaaga ggtggaggc gggagacaa gacgatagg 
      caacagtac aacgattat ggagggggt ggtggtaat 
      gaggcggag gttttagtg ccgagacag gctgtgggg 
      gttgttatt ggaaaaggg ggcgatatg attaagaaa 
      attcaagcg gaaactggg gcgcgggtt cagttccag 
      caagccaga gaggagggg ccgggggag aggcggtgc 
      tacctctca ggcaccccc aaacaagtc gaacaggct 
      agacaacga atagaggaa cttatcgac agcgtccat 
      cgacgagac ggcggcgac ggtcccggc caaggccgt 
      ggcagagga agaggtggc gatcgttac gacagccga 
      ggtggaagt aacagaaac ggcggagga caagactac 
      ggcggttgg gacgacaga agacaacaa caagcacct 
      caagaagtc acattcgtt gttccaagt tcgaaatgt 
      ggagtaata atcgggaga ggtggcgaa acgatcaaa 
      cagatcaat caacagtcc ggtgcccac tgtgagtta 
      gaccgccga tcacaaaac aatcagaat tcaaacgaa 
      aaaacattt ataatccgc ggcgatcct gatcaaatc 
      gaagcggcc aaacgaata atcagcgat aaagtccaa 
      atgcctctt aacttcgta cctgttggc ggtcctgca 
      atgtcaaac aacatgcaa actgcttat cctggaatg 
      gcgcctcaa tcttacaac ccccaaaat tggggcatg 
      cctgcttac caacaacaa tggaacgcc ccctcgcaa 
      agtgccgac gcccccaca caacaaaac gccatgcag 
      gttaacccc tcaactgga cagcctgac tactcgcta 
      cagtgggca gagtattac aggtcctta ggaatgcac 
      agggaagcg gaaatgata gaacaacag gcaaaatcg 
      aagggaatg tcctcgtcg gcccccggc caaccccaa 
      gccggtgcg acggcaggt tccggtcca gcacctgca 
      gcaaccccc actggtgct gcatccaat ggtggccaa 
      ccagattac agtgcccag tgggcggag tattatcgt 
      agtatagga aaacataaa gaagctgaa gcgatagaa 
      gcacaaatg aaatcgaag caacagtcc caagccaac 
      gtgcaatcg atgcaagga ggcccagct tcgtcccag 
      tccggccct gtccaagcc ccagcaggt ggttaccag 
      cagcaagcc tatggtgca tatcaagct tcaagtggt 
      tactacggt ggacaggcg caaggtggg gtccccagc 
      aacgtcccc caagggggc tacggcttc ccggcgtac 
      ggttacgga gcacccgcc cctgcagcc ccccctaac 
      aactccgac aactaa
    mRNA sequence
    • >TC009450 RA
      cacgtttgt acgcgggcg actgggcga caccatttt 
      ctgttttct atgtggaca aaattttat taatggtga 
      taaaaaatg agtgattat tcagcggta gcgccgcca 
      cctcaacag aattttggt ccttcctcg gcttttgcg 
      gccgccctc caaagagca aaacaaatc gctgctaaa 
      attaaccca ggatccaca aacccggcc gataatcga 
      ccgaaaagg cctctagaa gacgggcca gaaccggat 
      gcgaagaaa ggtccaagc accagtgga ccacctcct 
      caactgcct ccgcctgga aacagaccg ccgccgagc 
      cagggtggc atgggtgga cccccaatg ggtggcggg 
      ccgcagctt aatgaagac atcaaagtg cctgataaa 
      atggttggc ttaataatt ggtcgtgga ggcgaacag 
      atcacgaga cttcagtcg gaatcaggc tgtaaaatc 
      caaatggca cctgattca cagggaatg ccagaccgt 
      gtatgttcg ctatcaggc actaaagag gccatcaat 
      agagccaag gagctcatt atgaatatc gttcatcaa 
      agaggtcgc acggaaggg ttgggtggg ctcgatcta 
      gggccaaat atgggtcac aataattac ccagggatg 
      aacaacatg aaccagggg atgggtggt ggtggcggc 
      ggcggcggt ggcggtggc ggcggccgt aacttcgtc 
      gaaatcatg atacctgga cccaaagtc ggtctgata 
      atcggtaaa ggtggcgaa accatcaaa caactgcag 
      gaaaaaagc ggcgcgaaa atggttgtg attcaagac 
      ggtcctaat caagaacaa gaaaaaccg ttacggatc 
      agtggtgac ccttccaaa gtcgaatac gccaaacaa 
      ttagtctac gatttgatt gccgagaag gagatgcaa 
      aattataat cgaagaggt ggaggcggg agacaagac 
      gataggcaa cagtacaac gattatgga gggggtggt 
      ggtaatgag gcggaggtt ttagtgccg agacaggct 
      gtgggggtt gttattgga aaagggggc gatatgatt 
      aagaaaatt caagcggaa actggggcg cgggttcag 
      ttccagcaa gccagagag gaggggccg ggggagagg 
      cggtgctac ctctcaggc acccccaaa caagtcgaa 
      caggctaga caacgaata gaggaactt atcgacagc 
      gtccatcga cgagacggc ggcgacggt cccggccaa 
      ggccgtggc agaggaaga ggtggcgat cgttacgac 
      agccgaggt ggaagtaac agaaacggc ggaggacaa 
      gactacggc ggttgggac gacagaaga caacaacaa 
      gcacctcaa gaagtcaca ttcgttgtt ccaagttcg 
      aaatgtgga gtaataatc gggagaggt ggcgaaacg 
      atcaaacag atcaatcaa cagtccggt gcccactgt 
      gagttagac cgccgatca caaaacaat cagaattca 
      aacgaaaaa acatttata atccgcggc gatcctgat 
      caaatcgaa gcggccaaa cgaataatc agcgataaa 
      gtccaaatg cctcttaac ttcgtacct gttggcggt 
      cctgcaatg tcaaacaac atgcaaact gcttatcct 
      ggaatggcg cctcaatct tacaacccc caaaattgg 
      ggcatgcct gcttaccaa caacaatgg aacgccccc 
      tcgcaaagt gccgacgcc cccacacaa caaaacgcc 
      atgcaggtt aacccctca actggacag cctgactac 
      tcgctacag tgggcagag tattacagg tccttagga 
      atgcacagg gaagcggaa atgatagaa caacaggca 
      aaatcgaag ggaatgtcc tcgtcggcc cccggccaa 
      ccccaagcc ggtgcgacg gcaggttcc ggtccagca 
      cctgcagca acccccact ggtgctgca tccaatggt 
      ggccaacca gattacagt gcccagtgg gcggagtat 
      tatcgtagt ataggaaaa cataaagaa gctgaagcg 
      atagaagca caaatgaaa tcgaagcaa cagtcccaa 
      gccaacgtg caatcgatg caaggaggc ccagcttcg 
      tcccagtcc ggccctgtc caagcccca gcaggtggt 
      taccagcag caagcctat ggtgcatat caagcttca 
      agtggttac tacggtgga caggcgcaa ggtggggtc 
      cccagcaac gtcccccaa gggggctac ggcttcccg 
      gcgtacggt tacggagca cccgcccct gcagccccc 
      cctaacaac tccgacaac taagactct acgtgtaag 
      taaaaattt tgcttgtat ataatacgg acgtgtcgc 
      ttcctta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY